50 results about "Whitmania pigra" patented technology

The invention discloses a DNA (deoxyribonucleic acid) barcoding based molecular identification method and CO I sequence of a leech (whitmania pigra). Polymerase chain reaction (PCR) is carried out by using the method to amplify a CO I gene of the leech, a PCR product is sent to a sequencing company to be sequenced after being verified by agarose gel, the sequencing results are subjected to manual proofreading and sequence splicing and are compared with public sequences, and the tissue to be tested can be judged to be the source of whitmania pigra if the homology of the sequencing results and a gene sequence SEQ ID NO.1 is above 99%. The whitmania pigra variety is quickly and accurately identified by adopting the reliable DNA molecular identification technology, thus improving the accuracy and the reliability and ensuring the safety of the leech as a medicine.

The invention provides a high-efficiency substrate for artificially hatching Whitmania pigra Whitman spawns and aims to provide a substrate for hatching Whitmania pigra Whitman with shorter incubation time, higher hatching rate and more concentrated seedling emergence compared with the conventional hatching technique. By using the substrate with the best moisture holding capacity, the hatching time can be shortened by 7 days compared with the conventional hatching time, the hatching rate is increased by 22.7 percent, and the seedling emergence is concentrated. The substrate not only is beneficial to factory management for hatching the Whitmania pigra Whitman, but also prolongs the feeding time of Whitmania pigra Whitman seedlings, the adaptive capacity of the Whitmania pigra Whitman to the environment and the survival rate are improved through ingestion, growth and energy storage of the Whitmania pigra Whitman seedlings as soon as possible, the per mu yield of the Whitmania pigra Whitman is increased indirectly, and the economic benefit of culturing the Whitmania pigra Whitman is increased. By using the substrate, the per mu yield of the Whitmania pigra Whitman can be increased by about 15kg indirectly; and based on 600 RMB/kg of the current market price, the economic benefit of the Whitmania pigra Whitman per mu is increased by 6,000 to 9,000 RMB.

The invention relates to a traditional Chinese medicine preparation for treating cerebral thrombosis. The traditional Chinese medicine preparation is prepared from the following active pharmaceutical ingredients in parts by weight: 10-20 of radix angelicae sinensis, 25-35 of leguminosae, 10-20 of salvia miltiorrhiza, 10-20 of uncaria macrophylla wall., 10-15 of whitmania pigra whitman, 10-15 of rhizoma gastrodiae, 10-15 of radix paeoniae rubra, 10-20 of radix cyathulae, 10-20 of pericarpium citri reticulatae, 10-20 of radix ophiopogonis and 10-20 of fructus crataegi. Preferably, the traditional Chinese medicine preparation is prepared from the following active pharmaceutical ingredients by weight: 15 grams of the radix angelicae sinensis, 30 grams of the leguminosae, 15 grams of the salvia miltiorrhiza, 15 grams of the uncaria macrophylla wall., 12 grams of the whitmania pigra whitman, 12 grams of the rhizoma gastrodiae, 12 grams of the radix paeoniae rubra, 15 grams of the radix cyathulae, 15 grams of the pericarpium citri reticulatae, 15 grams of the radix ophiopogonis and 15 grams of the fructus crataegi. When treatment is carried out, the active pharmaceutical ingredients are taken and added with appropriate of water to be decocted for about 40 minutes at slow fire, and dredges of the decoction are filtered to obtain decoction. The decoction is orally taken, one dose is taken every day, and 15 days are a treatment course. The traditional Chinese medicine preparation is rich in formula medicine resources, is low in price and has an obvious curative effect.

The invention provides a method for imitative-ecological breeding of Whitmania pigra through paddy field net cages. The method comprises the following steps of 1, selection and preparation of paddy fields, 2, design and arrangement of the net cages, 3, arrangement of water inlets and water outlets, 4, planting of water plants in breeding ditches, 5, foodstuff feeding, 6, putting of Whitmania pigra fingerlings and 7, breeding management of the Whitmania pigra. According to the method, the Whitmania pigra is intensively bred with high density through the net cages, aquatic plants are used for purifying water and providing habitats for the Whitmania pigra, and the problem that the survival rate and catching rate of the Whitmania pigra in current breeding process are low is solved; and the cost for digging breeding ponds can be saved, the ecological environment is not damaged, management is convenient, the utilization value of the paddy field is played to a great extent, and the steady yield and high yield of the Whitmania pigra are ensured.

The invention discloses a preparation method for a whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity. The method comprises the following main steps: (1) degreasing whitmania pigra Whitman tissue; (2) performing enzymolysis on whitmania pigra Whitman protein; (3) separating whitmania pigra Whitman polypeptide; (4) performing chelation reaction on whitmania pigra Whitman polypeptide subjected to enzymolysis and zinc sulfate heptahydrate; and (5) performing alcohol precipitation separating and washing on the chelation reaction product. The prepared whitmania pigra Whitman polypeptide zinc chelate possesses relatively high yield and chelation rate, and oxford-cup-method bacteriostasis detection test proves that the whitmania pigra Whitman polypeptide zinc chelate possesses obvious antibiosis biological activity on escherichia coli and bacillus subtilis, and possesses great application prospect in the field of food and medicines.

The invention relates to the technical field of aquaculture, and discloses an overwintering device for whitmania pigra culture. The device includes a breeding box, a box cover is hinged to an openingin the upper end of the breeding box, heating lamps are symmetrically mounted on the box cover, a light brightness regulator electrically connected to the heating lamps is mounted at the upper end ofthe box cover, fixing plates are symmetrically and fixedly connected to the opposite inner box walls of the breeding box, positioning rods are fixedly connected to the upper ends of the fixing plates,a net cage is arranged between the two fixing plates, side plates are fixedly connected to the two sides of the net cage, positioning holes are formed in the side plates, the positioning rods are sleeved with the side plates through the positioning holes, and abutting plates are symmetrically and fixedly connected to the inner cage walls of the net cage. According to the overwintering device forwhitmania pigra culture, whitmania pigra in the breeding box can be conveniently taken out, the interior of the breeding box is easy to clean, the culture environment in the breeding box is better, and culture of whitmania pigra is facilitated.

The invention relates to the field of whitmania pigra hatching, in particular to a hatching and opening integrated technology for whitmania pigra. The method includes the following steps that 1, a hatching room is constructed; 2, a hatching box is placed; 3, whitmania pigra egg capsules are selected; 4, pomacea canaliculata egg masses are put for hatching for 4-7 days; 5, the whitmania pigra egg capsules are put for continuous hatching. The hatching and opening integrated technology for whitmania pigra realizes soilless hatching; compared with a traditional hatching technology, the larva emerging time of the whitmania pigra is more concentrated, collecting and transferring are also more convenient, management is convenient, and the survival rate is higher; meanwhile, pomacea canaliculata is hatched in the hatching box in advance, opening is directly performed after hatching and larva emerging of the whitmania pigra, culture techniques are reduced, and labor is saved.

The invention discloses a method for controlling pomacea canaliculata by utilizing medicinal whitmania pigra. The method includes that: the medicinal whitmania pigra is used as an object; a mixing culture and killing experiment of the pomacea canaliculata and the medicinal whitmania pigra, a weight gain experiment of the medicinal whitmania pigra, and a difference contrast experiment of predation of the medicinal whitmania pigra on male and female individuals of the pomacea canaliculata are performed based on life habits that the medicinal whitmania pigra feeds on sap sucked from mollusks such as viviparid, clam, and corbicula fluminea;the difference contrast experiment of predation of the medicinal whitmania pigra on the male and female individuals of the pomacea canaliculata shows that: when the predation number difference of the medicinal whitmania pigra fluctuates between 0 and 0.4, the predation of the medicinal whitmania pigra on the pomacea canaliculata has no gender difference; when the number and the density of the pomacea canaliculata are large, the number ratio of the the pomacea canaliculata to the medicinal whitmania pigra is more than 3 : 1, the pomacea canaliculata eats dead bodies of the medicinal whitmania pigra, but in this environment, the accumulation predation number of the medicinal whitmania pigra on the pomacea canaliculata reaches maximum, and it verifies that the certain control effect of the medicinal whitmania pigra on the pomacea canaliculata can be achieved.

The invention relates to a traditional Chinese medicine composition for treating gout and a preparation method thereof. The Traditional Chinese medicine composition comprises the following material components: coptis chinensis, cortex phellodendri, mung bean, rhizoma smilacis glabrae, lithospermum erythrorhizon, purple perilla, semen coicis, radix paeoniae rubra, reynoutria japonica, taraxacum mongolicum, dioscorea bulbifera, tulipa edulis bak, chaenomeles sinensis, radix cyathulae, ramulus mori, whitmania pigra whitman, rhizoma sinomeni cauliset, dioscorea tokoro, mirabilite and sodium sulfate. The raw materials are matched with one another according to weight, and finally form the traditional Chinese medicine composition with the efficacies of clearing away heat and promoting dieresis, removing heat from the blood, activating the channels, relieving internal fever and removing food retention, and the composition has good curative effect on gout, and ensures that the total effective rate reaches 80 percent. The traditional Chinese medicine composition is scientific in compatibility of medicines, obvious in curative effect, and rapid to take effect, and does not have toxic and sideeffect. The preparation method is simple, practicable, mild in conditions, good in stability and easy to promote.

The invention discloses a rice field crayfish and whitmania pigra symbiotic breeding method. The method comprises the following steps: step 1, conducting planting and breeding time sequence arrangement on rice, crayfish and whitmania pigra; step 2, performing transformation of rice field engineering: digging matts-shaped ditches in the field; step 3, establishing a grass-rice mussel-spirillum ricefield composite ecological system to provide natural baits for crayfishes and whitmania pigra; step 4, performing crayfish culture and catching; step 5, coupling rice planting management and whitmania pigra culture; step 6, performing whitmania pigra fry stocking preparation and fry putting; and step 7, performing self-making of a whitmania pigra compound feed, and supplementing artificial feed to a self-made cunninghamia lanceolata bait casting device. According to the method, through continuous experimental exploration, the success of breeding the crayfish whitmania pigra in the rice fieldis achieved, although the yield per unit of the whitmania pigra is not high, the crayfish whitmania pigra can be bred in the contiguous rice field, and batch production of the crayfish and the whitmania pigra is achieved.

The invention provides a natural additive for improving the anti-heat stress ability of whitmania pigra whitman.The invention aims to provide a breeding method for remarkbly enhancing the immunity ofthe whitmania pigra whitman, improving the anti-heat stress ability of the whitmania pigra whitman, promoting the growth of the whitmania pigra whitman, and increasing the economic benefit on the basis of existing whitmania pigra whitman breeding.Compared with an existing indoor high-density breeding technology for the whitmania pigra whitman, through the adoption of the natural additive, the survival rate of the whitmania pigra whitman is increased by about 40% maximally under heat stress, and the yield of the whitmania pigra whitman is increased by about 65% maximally at the same time.The economic benefit of whitmania pigra whitman breeding is increased without expanding the breeding area of the whitmania pigra whitman.

The invention provides a preparation method of a hirudin crude extract. The technical scheme is as follows: by using Whitmania pigra as the raw material, selecting, cleaning, refrigerating, crushing, precipitating, centrifugating and ultrafiltering to prepare the polypeptide compound product hirudin. The hirudin has the anticoagulation action, can be used for improving the treatment effect on neoplastic or thrombotic diseases in clinical veterinary medicine, and is especially suitable for neoplastic or thrombotic diseases of dogs, cats and other animals. The refrigerating crushing and ultrafiltration techniques are combined to extract the hirudin crude product, thereby ensuring the product quality on the premise of controlling the temperature. The method is suitable for the demands of mass production, can easily implement automatic production, and enhances the production efficiency. The prepared hirudin product lays a foundation for further purification and clinical application. The product can be used for neoplastic or thrombotic diseases of cats, dogs and other pets, effectively improves the state of illness, and provides a novel biological product for the pet market.

The invention discloses an egg breeding method for whitmania pigra and belongs to the technical field of aquatic product breeding. The egg breeding method for the whitmania pigra comprises the following steps of preparation of a breeding place, selection of breeding whitmania pigra, disinfection of the breeding whitmania pigra, mating and fertilization and egg cocoon incubation. The breeding place facilitates incubation and growth of the whitmania pigra, the breeding success rate of the whitmania pigra can be remarkably increased, an incubator can provide a reliable growth environment for the whitmania pigra and improve the breeding efficiency of the whitmania pigra, a modified composite film is laid in a cement breeding pond and used for adsorbing Cu and Cd heavy metal in soil, the safety of the soil is guaranteed, the incubation survival rate of egg cocoons is made to reach 92%, the quality of young whitmania pigra is improved, the breeding risk is reduced, and great economic benefits are achieved for healthy development of the artificial breeding industry of the whitmania pigra.

The invention discloses a whitmania pigra bait which comprises the following components by weight percent: 60%-80% of clam meat, 1%-3% of fish-dissoluble grease, 1%-3% of maltodextrin, 0.2%-0.5% of agar powder, 1%-4% of skim milk powder, 0.5%-2% of cassava starch, 0.04%-1% of lactic acid bacteria, 0.02%-1% of glycine, 0.04%-1% of phenylalanine, 0.1%-1% of compound premix and 15%-25% of water, wherein the compound premix comprises 3.93%-17.65% of compound microelements, 5.55%-14.515% of multi-vitamins, 48%-80% of growth factor and the balance of zeolite powder; the compound microelements are two or more of blue copperas, zinc sulphate monohydrate, ferrous sulfate monohydrate, manganous sulfate monohydrate, potassium iodide, sodium selenite and cobalt chloride; the multi-vitamins are two or more of vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, pantothenic acid, folic acid, biotin and 50% choline chloride; and the growth factor is one or two of corn gluten meal and DL-methionine.

The invention discloses a wide-body golden leech device suitable for multi-layer three-dimensional industrial cultivation, which includes a breeding box, and the inside of the breeding box is provided with a sewage collecting ditch and a breeding tank, and the sewage collecting ditch is located on one side of the breeding tank Below, the inner bottom of the culture tank is fixed with three attached partitions, and the three attached partitions are arranged at equal intervals along the horizontal direction, and the inner bottom of the sewage collection ditch is provided with a sewage outlet, and the inside of the sewage outlet is fixed with a sewage discharge port. The sewage discharge pipe is located on one side of the middle wall attached to the partition, and the sewage discharge pipe includes a pipe wall section with holes and a solid pipe wall section, and the pipe wall section with holes is located on one side of the solid pipe wall section. The present invention provides The pluggable water level control sewage pipe composed of a perforated pipe wall section and a solid pipe wall section can discharge different sizes of sewage in the breeding tank according to different water levels by changing the insertion end of the sewage pipe. Purify water quality without affecting the growth of cultured animals.

The invention discloses special feed for breeding whitmania pigra and a preparation method of the special feed. The special feed is prepared from the following raw materials in parts by mass: 15 to 25 parts of fresh field snail meat, 30 to 40 parts of fresh pomacea canaliculata meat, 5 to 10 parts of fresh scallop pallium, 3 to 5 parts of agar powder and 30 to 40 parts of distilled water. The preparation method comprises the following steps: firstly, adding the agar powder into the boiling distilled water, stirring to enable the agar powder to be fully dissolved, and then adding the fresh field snail meat, the fresh pomacea canaliculata meat and the fresh scallop pallium, and enabling the temperature of a mixture not to be lower than 50 DEG C; secondly, sufficiently crushing a mixture by a high-speed homogenizer, and then pouring the crushed material into a mold with a proper size and cooling at room temperature, thus obtaining the special feed for the whitmania pigra. The special feed for the whitmania pigra, disclosed by the invention, has the advantages that a solid form of the feed can be kept in water for 8 to 10 hours, and 50 to 60 percent of live freshwater snail can be replaced in the breeding process of the whitmania pigra.

The invention discloses an ecological method for planting rice and culturing leeches, and belongs to the field of ecological culture of aquatic products. According to the method, rice planting basins are distributed in a culturing water body for whitmania pigra, the rice is planted in the basins, and submerged plants are planted around the culturing water body; the rice and the submerged plants continuously absorb organic matter and residual baits in the water body, inhibit the growth of the weeds and mosses in the culturing water body, and provide attachments for whitmania pigra; meanwhile, the rice shields sunlight to reduce water temperature, so that the leaches spend the high-temperature period conveniently and safely. According to the ecological method for planting the rice and culturing the leeches, by utilizing the mutualism relationships among the submerged plants, the rice and the leeches, the rice and the submerged plants are utilized to improve the growth environment of the leeches, the growth of whitmania pigra is promoted, the ecological and healthy culture of the rice and the leeches is achieved, and the economic and ecological benefits of culture are increased.

The invention relates to a preparation method of a whitmania pigra whitman extract sustained-release pellet. The method comprises steps of preparing a drug-loaded pellet from a whitmania pigra whitman extract, and sequentially covering a swelling coating, a controlled-release coating and an enteric coating outside the drug-loaded pellet. The sustained-release pellet disclosed by the invention is a sustained-release preparation that pH control and time control are combined; and by virtue of the sustained-release preparation, the controlled release of the contained whitmania pigra whitman extract is delayed to the end of small intestine and to the part of colon, so that the effective components of the whitmania pigra whitman are protected from being damaged by various proteases in digestive tract and the bioavailability of medicines is enhanced.

The invention relates to a whitmania pigra breed reservation method, especially to a winter low-temperature breed reservation method of whitmania pigra. Influence of outside environment on overwintering of whitmania pigra is changed to solve problems in habitat and thermal insulation, an overwintering container is novel in design, and thus, the overwintering survival rate of whitmania pigra is improved greatly, and the emaciation rate is reduced. Overwintering technology of whitmania pigra is explored deeply and created so as to solve the problem of increasing shortened and degenerated seed sources in the scaled whitmania pigra culturing process, the cost is reduced for farmers, profit is provided, and the present situation of shortage of whitmania pigra can be changed.

The invention discloses a whitmania-pigra-whitman egg-cocoon floating incubating bed and an application method thereof. The incubating bed comprises a bed body and a cocoon containing box; the bed body comprises a bed edge, a bottom supporting frame and a top cover; the bed edge is cuboid, the length of the bed edge is 50 cm to 100 cm, the width of the bed edge is 30 cm to 60 cm, and the height of the bed edge is 5 cm to 15 cm; the bottom supporting frame is fixed at the bottom of the bed edge; the height of the bottom supporting frame is 1/3 height of the bed edge; the top cover is fixed at the top of the bed edge; the cocoon containing box is cuboid, and comprises a frame and a bottom face, and the bottom face is a net. When the whitmania-pigra-whitman egg-cocoon floating incubating bed is used, egg cocoons are put into the cocoon containing box, then the cocoon containing box is put into the bed body, and the whole bed body is put into a breeding pond of whitmania pigra whitman. The incubating bed is simple in structure and convenient to use, and the stable incubating environment is provided for the whitmania pigra whitman; the survival rate of the whitmania pigra whitman is increased.

The invention belongs to the field of polypeptides, and relates to an application of a whitmania pigra anticoagulant factor XIa polypeptide and a mutant thereof. A Kunitz type polypeptide sequence is screened from a salivary gland transcriptome database of whitmania Pigra, construction expression, screening and amino acid replacement are performed on the Kunitz type polypeptide sequence to obtain a series of polypeptides, and the polypeptides serve as prodrugs or are used for preventing and treating brain injury (such as paralysis and stroke) and other diseases. The polypeptide has the characteristics of safety, effectiveness and the like and is suitable for research and development of antithrombotic drugs.

The invention discloses a whitmania pigra inhabiting and feeding ground. Using the inhabiting and feeding ground disclosed in the invention, food organisms for the whitmania pigra such as snails are put in a groove body device; the whitmania pigra feeds on the snails in the device; the snail shells are gathered in the groove body device; after feeding on the snails, the whitmania pigra is adsorbedto an inhabiting plate to take a rest; due to shielding of a herringbone sunshade above a top longitudinal brace, the whitmania pigra is not disturbed by the sunshine and can be inhabited nearby quietly, the adsorbing and inhabiting space of the whitmania pigra is increased, and the whitmania pigra does not need to swim in long distance and does not need to search for a habitat again; the snail shells in the groove device body are cleaned regularly, fresh and live snails are fed to the whitmania pigra, the water quality in a water area is kept good to meet the requirements of the whitmania pigra for the water quality, the quantity of the whitmania pigra per unit area is increased, the quality is good, and the risk of disease infection is reduced; and the design is simple, the use is convenient, the effects are good, and popularization value exists.

The invention discloses a net cage breeding method for whitmania pigra. The breeding method includes the steps of preparing a net cage facility, installing net cages, preparing a cocoon producing bed, breeding management and collecting and hatching whitmania pigra cocoons. The net cage is reasonable in structural design, good breed is selected, the design method of the breeding process is reasonable, operation is easy and practical, cost is low, the whitmania pigra breeding effect is improved substantially, and economic benefits are substantially increased.

The invention provides a three-dimensional planting and culturing method for alisma orientalis, whitmania pigra and hyriopsis cumingii which not only can improve the yield and survival rate of whitmania pigra, but also can solve the problem of low uniformity of hyriopsis cumingii, can also increase the yield of alisma orientalis and pearls, and can improve the space utilization rate and economic benefit of a pond. According to the three-dimensional planting and culturing method for the alisma orientalis, the whitmania pigra and the hyriopsis cumingii, in September of the year, three traditional Chinese medicinal materials including the alisma orientalis and the pearls can be harvested while the whitmania pigra is harvested, the space utilization rate of a pond is effectively increased, compared with a whitmania pigra single feeding mode, the three-dimensional planting and culturing method increases the yield and survival rate of the whitmania pigra, can increase the yield of the alisma orientalis and the pearls, can solve the problem of low uniformity of the hyriopsis cumingii and improve the space utilization rate and economic benefits of the pond. The three-dimensional planting and culturing method is suitable for popularization and application in the aquatic traditional Chinese medicine cultivation field.