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Egg breeding method for whitmania pigra

A wide-bodied golden leech and egg cocoon technology, which is applied in the field of aquatic breeding, can solve the problems of leech survival threat, vulnerability to climate conditions, time-consuming and labor-intensive problems, etc.

Active Publication Date: 2021-10-08
ANHUI GUANCHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional method of raising seedlings has many disadvantages, for example: cocoon production and incubation platforms built on farmland soil are difficult to control in terms of moisture retention, air permeability, softness, and insect pests; the workload is large, time-consuming and laborious; it is easily affected by climate conditions. impact, the emergence rate is low, and the seed production stability is poor; more importantly, there are bacteria, microorganisms, pesticide residues and heavy metal residues in the farmland soil, which will threaten the survival of the species of leech, so it is necessary to develop a wide-body golden leech Advanced egg breeding methods to ensure the safety of eggs and the quality of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The modified composite membrane is made by the following steps:

[0030] Step S1, the 60.5mgFe 3 o 4Disperse in 40mL of ethanol solution with a mass fraction of 95%, add 0.3mL of ammonia water with a mass fraction of 28%, disperse ultrasonically for 20min, and then place it in a water bath at 45°C and stir for 1h to obtain a mixed solution a. Dissolve 1.5mL of butyl titanate Dissolve in 20mL of absolute ethanol to obtain mixed solution b; drop mixed solution b into mixed solution a, mix and react for 24 hours to obtain solid particles through magnetic separation, and wash the solid particles twice with deionized water and absolute ethanol respectively, get Fe 3 o 4 @TiO 2 Microspheres;

[0031] Step S2, the 60.2mgFe 3 o 4 @TiO 2 Put the microspheres into the reaction kettle, add 40mL of 1mol / L sodium hydroxide solution, raise the temperature to 140°C for 2h, centrifuge to obtain the precipitate, wash the precipitate twice with deionized water and absolute ethano...

Embodiment 2

[0037] The modified composite membrane is made by the following steps:

[0038] Step S1, the 61.5mgFe 3 o 4 Disperse in 43mL of ethanol solution with a mass fraction of 95%, add 0.35mL of ammonia water with a mass fraction of 28%, ultrasonically disperse for 20min, and then place it in a water bath at 45°C and stir for 1h to obtain a mixed solution a. Dissolve 1.7mL of butyl titanate Dissolve in 23mL of absolute ethanol to obtain mixed solution b; drop mixed solution b into mixed solution a, mix and react for 25 hours to obtain solid particles through magnetic separation, and wash the solid particles twice with deionized water and absolute ethanol respectively, get Fe 3 o 4 @TiO 2 Microspheres;

[0039] Step S2, the 60.8mgFe 3 o 4 @TiO 2 Put the microspheres into the reaction kettle, add 41mL of 1mol / L sodium hydroxide solution, raise the temperature to 140°C for 2h, centrifuge to obtain the precipitate, wash the precipitate twice with deionized water and absolute etha...

Embodiment 3

[0045] The modified composite membrane is made by the following steps:

[0046] Step S1, the 62.5mgFe 3 o 4 Disperse in 45mL of ethanol solution with a mass fraction of 95%, add 0.4mL of ammonia water with a mass fraction of 28%, disperse ultrasonically for 25min, then place it in a water bath at 45°C and stir for 2h to obtain a mixed solution a, dissolve 2mL of butyl titanate In 25mL of absolute ethanol, the mixed solution b was obtained; the mixed solution b was dropped into the mixed solution a, and the solid particles were obtained by magnetic separation after the mixed reaction for 26 hours, and the solid particles were washed 3 times with deionized water and absolute ethanol to obtain Fe 3 o 4 @TiO 2 Microspheres;

[0047] Step S2, the 61.5mgFe 3 o 4 @TiO 2 Put the microspheres into the reaction kettle, add 42mL of 1mol / L sodium hydroxide solution, raise the temperature to 140°C for 3h, centrifuge to obtain the precipitate, wash the precipitate with deionized wat...

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Abstract

The invention discloses an egg breeding method for whitmania pigra and belongs to the technical field of aquatic product breeding. The egg breeding method for the whitmania pigra comprises the following steps of preparation of a breeding place, selection of breeding whitmania pigra, disinfection of the breeding whitmania pigra, mating and fertilization and egg cocoon incubation. The breeding place facilitates incubation and growth of the whitmania pigra, the breeding success rate of the whitmania pigra can be remarkably increased, an incubator can provide a reliable growth environment for the whitmania pigra and improve the breeding efficiency of the whitmania pigra, a modified composite film is laid in a cement breeding pond and used for adsorbing Cu and Cd heavy metal in soil, the safety of the soil is guaranteed, the incubation survival rate of egg cocoons is made to reach 92%, the quality of young whitmania pigra is improved, the breeding risk is reduced, and great economic benefits are achieved for healthy development of the artificial breeding industry of the whitmania pigra.

Description

technical field [0001] The invention belongs to the technical field of aquatic breeding and relates to a method for raising eggs of wide-bodied golden leeches. Background technique [0002] The wide-bodied golden leeches, belonging to the phylum Annelids, the class Verrucota, the orders Anospermia, the genus Chrysophyceae, and the genus Chrysalis, commonly known as leeches, are one of the three major breeding species of medicinal leeches in my country, and their dried products are traditional Chinese medicinal materials with special effects in my country. . There is a huge demand for medicinal leeches in the medicinal material market. Due to environmental pollution and artificial overfishing, wild resources are almost exhausted, and the production of wild leeches can no longer meet market demand. The work of artificial breeding of leeches broad-bodied can be roughly divided into: the introduction of leeches, the breeding of egg cocoons, the collection and incubation of egg c...

Claims

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Application Information

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IPC IPC(8): A01K61/40A01K61/17A01K63/00A01K63/04
CPCA01K61/40A01K63/003A01K63/006A01K61/17A01K63/04Y02A40/81
Inventor 李中华张翠丽惠晴晴李龙宇
Owner ANHUI GUANCHAN BIOTECH
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