79 results about "Spirillum" patented technology

An Azospirillum brasilense DraT-engineering strain containing multi-copied nifA gene is disclosed, with preserving number of CGMCC No.0876. This bacterial manure containing engineering strain of Azospirillum brasilense DraT- with multi-copied nifA is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

The invention relates to glucose-rich spirulina polysaccharide and an application thereof in a plant green pesticide and an anti-tumor drug. High-sugar spirulina obtained through nutritional regulation is used as a raw material and is subjected to hot water extraction, protein removal with a flocculant, concentration, precipitation and freeze drying to obtain the water-soluble high-sugar spirulina polysaccharide having the molecular weight of 10-200 kDa; the spirulina polysaccharide comprises the monosaccharides mainly comprising rhamnose, fucose, arabinose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, and is characterized in that the glucose content is more than 50% in the monosaccharide composition. The spirulina polysaccharide can obviously induce plant disease resistance, has obvious anti-tumor effect on tumor, and can be used as the biological pesticide and the anti-tumor drug.

A kit for quickly determining infection of polyloric spirillum consists of kit body, application reagent prepared from distilled water and 1% of urase solution, plastic reaction plate with round scrobicula on its surface and Huatemen test paper prepared by soaking said paper completely in 1% of urea solution and in phenol red indicator then drying it in the air.

The invention discloses a WXZ-14 CGMCC No. 1799 strain of Herbaspirillum huttiense with aerobic denitrifying property and application to dispose and denitrify waste water, which is characterized by the following: displaying strong tolerant degree for the dissolved oxygen; reducing nitrate and nitrite at obvious speed under 10-98% saturated oxygen dissolving condition; inoculating the strain in the active sludge to do enriching culture; realizing the goal of aerobic denitrification.

The invention provides three Th cell epitope peptide and code DNA of pylorus spirillum urease B subunit, also providing a bacterin of B cell epitope which contains the triepitope peptide and an additional urease B subunit. Among them, the epitope peptide is the polypeptide which has one of the amino acid residue sequences as follows: 1) hasing amino acid sequence of sequence 2, sequence3 and sequence 7 in sequence table; 2) replacing, deleting or appending the amino acid sequence of sequence 2, sequence3 and sequence 7 in sequence table by one or several amino acid residue to form derivanting polypeptide. The coded DNA is one of the following sequences: 1) hasing the ribonucleotide sequence of sequence 8, sequence 9 and sequence sequence 10 table. 2) Hasing the ribonucleotide sequence of same coded product with sequence 8, sequence 9 and sequence10 in sequence table. The epitope vaccine which contains the polypeptide of this invention has the protein vaccine of amino acid sequence in sequence 12 of sequence table. The vaccine or medication which is made by using the polypeptide of the invention as active component can clean out the infection caused by pylorus spirillum and has wide application prospect in medical realm.

The treatment of spirulina by a process which includes placing spirulina that has not been previously heat-sterilized, lactic acid bacteria and sugar in water, then culturing the lactic acid bacteria provides treated spirulina in which the distinctive taste and odor of spirulina are minimized, in which active ingredients such as phycocyanin remain intact, and which contains a reduced level of bacteria other than lactic acid bacteria.

The invention discloses a maca dunaliella salina compound preparation. The maca dunaliella salina compound preparation comprises dunaliella salina and spirulina and is prepared from the following materials: 3-10 percent of dunaliella salina, 40-45 percent of spirulina and 45-55 percent of maca. The maca dunaliella salina compound preparation has the effects of building body and tonifying Yang, curing organs, maintaining beauty and keeping young and improving the digestive system, achieves a two-way blood-pressure regulating function, as well as a two-way blood sugar regulating and balancing function, can regulate body metabolism and resist aging, tumor and fatigue and enhance the immunity, and has remarkable effects on cardio-cerebrovascular diseases, chronic diseases, senile dementia, climacteric syndrome and the like. When drunk frequently, the maca dunaliella salina compound preparation increases sexual intercourse, keep youth for a long time and prolongs the lifetime, has the advantages of relaxing tendons and activating collaterals, activating blood circulation to dissipate blood stasis, dispelling wind to eliminate dampness and strengthening bones and tendons and can remarkably improve memory.

The invention involves a kind to be good lowly, using the sea water cultivation effect bears the salty rapana thomasiana microbial inoculum using the microbial inoculum raise expense the application raise technology. Bears the salty red spirillum the selective breeding for to concentrate, the plate separation, the strain function selective breeding from the sea water, bears the salty red spirillum function strain. Using raise technology including culture medium optimization and application raise craft condition improvement. The culture medium optimization is from carbon source, nitrogen source, ingredient and so on inorganic salt and growth factor carries on the optimization, obtains reduces 47% culture medium ingredient the application formula. Uses the application formula postpositioned using the microbial inoculum raise, the vaccination under the white plastic warm awning the sunlight raise. The invention bears the salty rapana thomasiana strain to be possible to grow under the 0.1~~4%NaCl density, in the fresh water, the sea water cultivation may receive the good application effect. It greatly reduced the photosynthesis bacilliculture cost and the equipment using the raise technology invests. Bears the salty rapana thomasiana microbial inoculum the application raise to issue each milliliter hundred million cells in the above condition the use density to request.

This invention discloses the construction of a fused poloric spirillum adhesion gene engineered bacterial vaccine characterizing that the adhesion HpaA is used to construct an adjuvant fused engineered vaccine of poloric spirillum adhesion and colibacillus heat-labile enterotoxin B sub unit or cholear toxin B sub unit intramolecular to get large quantity of necessary vaccine proteins by high density fermentation, occlusion body extraction, renaturation and purification to be proprared to peroral, injection and spray agents.

A method and composition for stimulating the proliferation and differentiation of stem cells is used to self-repair injury in mammals. A supplement is administered having an effective dose of substances selected from blueberry, carnosine, catechin, green tea extract, VitaBlue, Vitamin D3, Spirulina, AFA or effective combinations and derivatives of these. For example, a supplement comprising wild blueberry, green tea extract, carnosine, vitamin D3, and a Spirulina and / or an AFA-Omega (EtOH) exhibited a synergistic and unexpected association with substantially increased proliferation of bone marrow stem cells and CD34+. A therapeutic amount of a substance that is associated with a substantial increase in stem cells may be used to prevent and repair damages tissues of the brain, due to stroke, the heart, due to coronary artery disease or a heart attack.

A bacteria agitation leaching method for a uranium ore belongs to the technical field of biological metallurgy. The method is conducted according to the following steps: (1) inoculating a mixed bacteria solution containing ferrous oxide micro-spirillum, eosino-ferrous oxide thiobacillus and eosino-thiobacillus thiooxidans into a culture medium to prepare a culture solution; (2) regulating the pH value, introducing air and stirring to culture so as to prepare a leaching bacteria solution; (3) adding water into uranium ore powder to prepare an ore pulp, regulating the pH value with sulfuric acid, then adding the ore pulp into the leaching bacteria solution to prepare a leaching mixture solution; (4) regulating the pH value of the leaching mixture solution by using the sulfuric acid, introducing air and stirring to leach. According to the method, the leaching rate of the uranium is remarkably improved, the acid consumption is reduced, and by improving dynamics in the mineral leaching process, the leaching period is shortened, and the leaching rate is increased. The method provided by the invention has the advantages of low production cost, low investment, simple equipment, environment friendliness and the like.

Compositions and methods are provided for novel guide RNA / Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.

Disclosed is a compound silkworm chrysalis polypeptide multifunctional food, which comprises (by weight ratio) the following raw materials, silkworm chrysalis polypeptides 15-35%, soybean polypeptide 30-60%, spirulina 10-25%, phosphatidy lcholine 5-10%, and starch 0-5%. The food has rather high nutritive value and biological activity.

The invention discloses a spirulina with active spirulina peptide and spirulina prolease and preparing method and application, which is characterized by the following: adjusting human blood fat; adopting bacillus subtilis to ferment spirulina; making the active spirulina peptide at 15-40% when the water content is under 7%; possessing 1000-40000U / g spirulina prolease.

A disclosed organic biological fertilizer for pomegranate trees is prepared from the following raw materials in parts by weight: 20-40 parts of rice bran, 10-15 parts of vinasse, 15-25 parts of peat, 15-25 parts of soybean cake powder, 15-20 parts of turf, 15-20 parts of corn straw, 10-20 parts of chicken manure, 10-15 parts of wheat bran, 5-7 parts of ferrous sulphate, 3-6 parts of ammonium sulfate, 6-8 parts of magnesium sulfate, 3-9 parts of borax, 4-7 parts of potassium fulvate, 2-6 parts of marine spirillum powder, 1-4 parts of bacillus megaterium, 2-5 parts of sporotrichum thermophile, 3-6 parts of microzyme, 4-7 parts of cellulase and 5-8 parts of lactic acid bacteria powder. The provided organic biological fertilizer for pomegranate trees is capable of improving output and sugar content of pomegranate without pollution.

The invention discloses a bacterial leaching copper method of complex chalcopyrite flotation tailings, comprising seven steps, namely granulation, bacterial culture, acidity adjustment, bacterial leaching, extraction, back-extraction and electro deposition. The method uses the mixed bacterial of thiobacillus ferrous oxide and micro spirillum ferrous oxide to carry out leaching on mill tailings taken as raw materials that can not be effectively and economically treated by other processes, and metal copper in the raw materials is recovered. Therefore, not only the situation of the shortage of copper resource is alleviated and favorable economic benefits are created, more important, the problem that copper tailings sand occupies a great amount of fertile land and pollutes environment is solved, and win-win benefits of economy and social environment are realized.

The invention provides a novel spirulina polypeptide P1 with bacteriostatic activity. An amino acid sequence of the spirulina polypeptide P1 is shown as Seq ID No.1. The spirulina polypeptide P1 with bacteriostatic activity is extracted from spirulina for the first time. Experiments demonstrate that the spirulina polypeptide P1 can effectively suppress gram-positive bacteria and gram-negative bacteria, has no toxic or side effect, can be used as a broad-spectrum antibacterial agent or a bacteria preservative, and has wide application prospects.

Compositions and methods are provided for novel guide RNA / Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.

The treatment of spirulina by a process which includes placing spirulina that has not been previously heat-sterilized, lactic acid bacteria and sugar in water, then culturing the lactic acid bacteria provides treated spirulina in which the distinctive taste and odor of spirulina are minimized, in which active ingredients such as phycocyanin remain intact, and which contains a reduced level of bacteria other than lactic acid bacteria.

The invention aims to provide a microbiological degradation method for nitrobenzene industrial wastewater. According to experimental selection, composite bacteria liquid of Pseudomonas, Stenotrophomonas maltophilia, Acinetobacter baumannii, Aquaspirillum, Acidovorax avenae, Alcaligenes faecalis and Bacillus is provided. Domestication culture and three-stage amplification culture are carried out on the composite bacteria liquid so as to obtain a high efficiency composite microbial inoculum suitable for industrial production. When used to treat nitrobenzene wastewater, the microbial inoculum provided in the invention has the advantages of strong biological activity, long survival time, high capability in resisting impact load of influent quality and thorough treatment.

This invention disclose a purification technology in the preparation of reconstitution pylorus spirillum heat shock protein A gene engineering vaccines applying pressure, filtration, Ni ions affinity chromatography and purification, concentration lysis, glue filtration chromatography and renaturation to get pure reconstitution pylorus spirillus heat shock protein A.

A microbial agent for promoting plant growth is prepared from brown azotobacterium, nitrogen-immobilizing spirillum, Bacillus megaterium and streptomycete through three-class culture of each strain and proportionally mixing. It can promote the absorption of plant to mineral elements in soil and suppress the root pathogens.

The invention relates to a spirulina polysaccharide eye drop, in particular to an eye buffer solution containing spirulina polysaccharide with effective active concentration. According to the invention, the spirulina polysaccharide is prepared into an eye drop for the first time and applied to prevention and treatment of bacterial keratitis. The eye drop disclosed by the invention has a simple and easy-to-implement preparation method, can obtain a remarkable effect in prevention and treatment of the bacterial keratitis, and has a wide application and development prospect.

The invention discloses a bacteria; the bacteria is prepared from, by weight, 32-56 parts of sarcina, 15-34 parts of spirillum, 20-35 parts of Brevibacillus laterosporus, 23-62 parts of Acinetobacter lwoffii, and 36-68 parts of Chryseobacterium indologenes. When the bacteria is applied to treat the VOCs waste gas, the treatment effect to VOCs is good, and the degrading efficiency is high.

The invention discloses a strategy and an application technology, wherein endogenous spirillum in a tea tree is used to promote selenium conversion and enrichment of the tea tree, so the content of organic selenium in tea leaves can be increased. Biological characteristics that the endogenous spirillum in the tea tree has reciprocal symbiosis with the tea tree and the selenium reducing capacity is high are used, and the endogenous spirillum in the tea tree is led into tea seedlings for fixed plantation, so that physiological poison effects of selenate absorbed by roots on the tea tree can be reduced; and selenium metabolism products are provided to the tea tree for direct use, so that enrichment of the organic selenium in the tea leaves can be promoted. By the technology, the selenium content in the tea leaves can be increase by two to three times in the tea tree which is planted in low-selenium soil or high-selenium soil or a tea garden with application of selenium fertilizer. According to the invention, technical processes designed according to field land tea tree cultivation can be operated easily; repeated inoculation and special management are not needed; and selenium enrichment effects are significant in the tea leaves.

The invention provides a spirulina nutrient solution, which is prepared by the following steps of mixing spirulina powder and water into a spirulina solution, adding the papain into the spirulina solution, carrying out enzymolysis at a temperature of 60 DEG C to 68 DEG C, and carrying out high-speed centrifugal filtration to obtain a clear and transparent spirulina nutrient solution. The spirulina nutrient solution can greatly enhance the direct utilization ratio of the spirulina protein, and can ensure that the spirulina nutrients including amino acid, vitamin B1, vitamin B2, Beta-carotene, blue-green algae, calcium, Fe, Zn, Mg and K are completely dissolved in the solution. Compared with solid spirulina tablets, capsules and powder, the spirulina nutrient solution is more easy to absorb and utilize by human bodies, so the spirulina nutrient solution can meet the nutrition requirements of the aged, infants, patients and most people and has better and faster positive effects on human body health care function. The spirulina nutrient solution of the invention can be prepared into various kinds of beverages or drinks, which is convenient for people of different ages and different physical conditions to drink.

The invention relates to compound sea cucumber intestine polypeptide powder and a preparation method thereof. The compound sea cucumber intestine polypeptide powder is prepared from, 30-40 parts of sea cucumber intestine polypeptide, 20-25 parts of spirulina powder, 10-15 parts of Chinese wolfberry fruit extract, 10-15 parts of ginseng extract, 5-10 parts of yam extract, 5-12 parts of red jujube extract and 12-18 parts of baker's yeast powder through processing. The preparation method of the compound sea cucumber intestine polypeptide powder comprises the following steps that 1, sea cucumber intestines are processed; 2, the sea cucumber intestines are homogenized; 3, primary enzymolysis is performed; 4, secondary enzymolysis is performed; 5, ultrafiltration is performed; 6, nanofiltration is performed; 7, concentrated drying is performed. The compound sea cucumber intestine polypeptide powder prepared by adopting the preparation method is rich in nutritional ingredient, easy to absorb and utilize, high in absorption and utilization rate, low in impurity and heavy metal content and high in nutritional value, can supplement a calcium element and can also complement a variety of nutrition elements from sea cucumber intestines and spirulina.

The invention discloses a biological environmentally friendly pesticide comprising the following components by weight: 1-2 parts of hirsutella thompsonii fisher powder, 4-6 parts of ocean spirillum powder, 3-4 parts of abamectin, 4-9 parts of matrine, 1-3 parts of a synergistic agent, 2-8 parts of perillaseed, 3-7 parts of hematoxylon, 1-3 parts of perilla leaf, 3-7 parts of almond, 4-8 parts of cortex eucommiae, 6-7 parts of holly root, 1-10 parts of a stabilizer, 2-8 parts of frankincense, 3-7 parts of herba eupatorii, 4-6 parts of rhizoma cibotii, 5-12 parts of herba lycopi, 11-15 parts of alisma and 100-150 parts of water. The biological environmentally friendly green pesticide is a new pesticide which is high-efficiency, purposeful, environmentally-friendly, free of secondary toxicity, residue and pollution, can keep ecological balance, is an important guarantee for realization of ecological agriculture, green ecological agriculture and green enterprise, and has broad prospects.