909 results about "Bacillus <bacterium>" patented technology

Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

A novel clostridia bacterial species (Clostridium coskatii ATCC No. PTA-10522, “PS02”) is provided. Under anaerobic conditions C. coskatii can convert CO and / or H2 and / or CO2 to ethanol or acetate. Thus, this novel bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

The invention discloses a microbiogenic cement and concrete and a production method thereof, which mainly mixes the Bacillus pasteurii solution for producing urease and an admixture of containing urea and mineral calcium salt and obtains the microbiogenic cement after reacting in a certain condition; adds admixture such as sand etc. into the mixture of mixing the bacterial solution and the mixture and reacts to generate the microbiogenic concrete. The microbiogenic cement or concrete of the invention can be used on protection and restoration of masonry ancient architectures and repair of cracks on concrete building or treatment of liquescent sandy soil. The invention provides a permanent method of safety, effectiveness and without environmental hazard for repair and strengthening technology of large quantity of civil architecture and structure; the invention has the advantages of the good effects, the good weatherability, the good permeability of gas and the good permeability of water and being especially suitable for treatment of the sandy soil; the invention is safe and environmental-friendly, and belongs to the environmental-friendly restoring material.

The invention discloses a method for repairing a crack of a cement-based material. According to the method, carbonic anhydrase microorganisms are adopted for generating carbonic anhydrase used for catching CO2, CO2 is promoted to be converted into CO3<2->, and the deposition of calcium carbonate within a surface area of the crack is accelerated; the method has the advantage that the repair speed is faster than that of other methods for repairing the crack through the microorganisms. The method comprises the following steps of: inoculating bacillus mucilaginosus to a culture medium; culturing; preparing bacillus mucilaginosus concentrated bacteria liquid; immobilizing bacteria on carriers; uniformly burying calcium sources into the cement-based material during forming the cement-based material; concentrating the carriers with the bacteria immobilized into a range of 5mm under a surface area of a test part; manufacturing the crack after a part to be tested is cured; transferring into a thermostatic waterbath; continuously charging air for maintaining and repairing. The repair test result shows that the osmotic coefficient is greatly reduced after repairing for 5 days, and the crack is repaired completely after continuously repairing for 30 days.

The present invention provides a bacterium belonging to the genus Bifidobacterium, by which DNA coding for a protein having an antitumor activity or DNA coding for a protein having the activity of converting a precursor of an antitumor substance into the antitumor substance is delivered to tumor tissues specifically under anaerobic conditions thereby expressing the protein encoded by the DNA, as well as a pharmaceutical composition comprising said anaerobic bacterium.

This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010 / mL, and that of Sphingobacterium multivorum is 107-1012 / mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.

The invention provides a plug-flow type bioleaching treatment process for sludge treatment and equipment thereof. The equipment comprises a sludge regulating reservoir, an activating tank, a plug-flow type bioleaching reactor, an advection type sludge concentrated tank, a heavy metal recovery pond and other facilities, an aeration device, a heating device, a dehydration device, a stirring device and other devices. The process comprises the following steps that: a sludge system is acidified by a composite bacterium consisting of a thiobacillus and an acid resistant heterotrophic bacterium under conditions of aerobism and existence of sulfur powder and other composite nutriments, heavy metal in the sludge is massively dissolved in liquid phase, the sludge is regulated at the same time so as to facilitate sedimentation and dehydration; the sludge stays in the reactor for 2 to 4 days; discharged treated sludge enters the concentrated tank to be subjected to gravity thickening; 20 to 50 percent of concentrated bioleaching sludge flows back to the reactor, and the rest sludge is subjected to chamber filtration and dehydration without adding any flocculating agent until the water ratio is below 60 percent; and the heavy metal in the water is recycled by an alkali precipitation method. The method can remove massive heavy metals of the sludge, has over 99 percent of sludge pathogen kill ratio, and ensures that the treated sludge does not have malodor and is in khaki color, thereby realizing the aims of innoxiousness and minimization of the sludge.

A process for converting a substrate such as carbon monoxide to useful chemicals has been developed. The process involves providing a substrate comprising CO to a bioreactor which contains a culture of one or more micro-organisms and anaerobically fermenting the substrate to produce 2,3-butanediol (BDO). The BDO is next converted to one or more of butane, butadiene and / or methyl ethyl ketone which in turn can be converted to other compounds. The source of the CO can be an industrial process such as the ferrous metal products manufacturing. The microorganism can be Clostridium autoethanogenum, Clostridium ljundahlii or Clostridium ragsdalei.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention discloses a microorganism compound microbial inoculum, the strains of which comprise bacillus, soil actinomycetes, microzyme, mildew and photosynthetic bacteria. By using the microbial inoculum for straw fermentation, a result indicates that the microbial inoculum can make straws quickly decay and transfer to organic fertilizer required for the growth of plants, thereby the content of soil humus is increased, and the content of microorganism and trace elements in soil is improved. The physicochemical property of soil is improved, and the capability of water and fertility preserving of soil is increased. The soil permeability can also be improved so as to inhibit the happening of crop pests, and the output and quality of products can be improved. The microbial inoculum has the advantages of convenient use, low cost and obvious effect.

The invention discloses a composite soil conditioner for treating heavy metal Cd pollution, and preparation and use methods thereof. The composite soil conditioner comprises the composition and content: 20-30% of clay minerals, 10-20% of peat, 10-20% of biochar, 25-35% of slaked lime, 5-15% of an oxide of iron, and 5-10% of a microbial agent. The preparation of the composite soil conditioner comprises the following steps: adding the clay minerals, the peat, the biochar, the slaked lime and the oxide of iron in proportion, then fully stirring until the raw materials are evenly mixed; adding the microbial agent with the amount of 5-10%, to prepare an inorganic and organic composite conditioner, wherein the microbial agent contains at least one of iron-reducing bacteria, photosynthetic bacteria and bacillus. The composite soil conditioner prepared by the method is directly added to crop field soil polluted by heavy metals in proportion, and then ploughed fallowing is performed. Compared with the prior art, the product has the advantages of ingenious and reasonable formula design, excellent in-situ passivation effect, simple preparation method, low production cost, simple application method and the like.

The invention discloses a method for synchronously removing nitrogen and phosphorus in sewage containing nitrogen and phosphorus, belonging to the field of sewage treatment. The method comprises the steps of (1) screening thiobacillus denitrificans: screening thiobacillus denitrificans from anaerobic activated sludge and taking as target strain, wherein thiobacillus denitrificans utilizes sulphur source in ferrous sulfide to conduct autotrophic denitrification; (2) preparing fillers of a reactor: directly adding ferrous sulfide with the particle size smaller than 25mm in a reaction container; (3) adding sewage: feeding sewage to be treated into the reaction container, wherein the sewage to be treated is the sewage containing nitrogen and phosphorus with the pH value of 5.0-9.0; (4) conducting the autotrophic denitrification process: conducting the mixed reaction for 2 to 6d at the temperature of 10 DEG C-40 DEG C under the anaerobic condition in the reaction container, reducing NO2<-> and NO3<-> in water to be N2 by thiobacillus denitrificans while simultaneously oxidizing ferrous sulfide by thiobacillus denitrificans, and simultaneously, precipitating phosphorus in water by utilizing iron ions (being oxidation products); and (5) separating solid and liquid. According to the method, the technology for removing nitrogen by utilizing thiobacillus denitrificans and the technology for removing phosphorus by using iron ions are fused naturally, the reaction cost is low, and the processing effect is good.

The invention provides a method for producing an organic fertilizer from municipal dry branch / fallen leaf waste and application thereof. The method comprises the following steps: collecting branches, fallen leaves and other municipal greening wastes, and mixing Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Trichoderma reesei to obtain a composite microbial inoculant; adding bran coat, chaff powder or peanut hull meal, the composite microbial inoculant, urea and potassium dihydrogen phosphate, thoroughly mixing, piling into a 1.5-2m-high cone, and controlling the water content at 60-70%; carrying out hide lifting on the pile in the composting process, and fermenting for 20-30 days; and carrying out aerobic and anaerobic fermentation on the dry branch / fallen leaf waste by using the microbial inoculant to obtain the organic fertilizer. The preparation technique is simple; and the prepared organic fertilizer can improve the soil and reduce the environmental pollution caused by the waste.

The present invention is directed to methods for delaying a plant development process comprising exposing a plant or plant part to one or more bacteria or enzymes. In specific embodiments, the one or more bacteria are selected from the group consisting of Rhodococcus spp., Pseudomonas chloroaphis, Brevibacterium ketoglutamicum, and a mixture comprising any combination of these bacteria. Apparatuses for delaying a plant development process comprising a catalyst that comprises one or more of the above bacteria.

The invention discloses a halophilic bacterial agent and a preparation method thereof as well as a biological treatment system fixed with the bacterial agent and application thereof. In the invention, strains are screened from sewage, sludge and soil to be prepared into a bacterial agent, wherein the bacterial agent comprises four strains including Providenciasp. CJ-4, Oceanobcillussp. CJ-10, Brevibacterium epidermidis CJ-12 and Halomonassp. CJ-13. The invention also provides the biological treatment system fixed with the bacterial agent and a method for treating waste water by using the same. The bacterial agent disclosed by the invention has stronger degradation capability on phenolic wastewater, can adapt to high-salt environments and has very good degradation effects under an environment with the salt content of 2-25% and the phenol concentration of 2500 mg / l; and compared with the traditional active sludge method, the bacterial agent has obvious advantages in the performance of degrading phenolic compounds, and the degradation effect on the phenolic compounds in water can reach more than 99.9%.

A crystalline form of crystalline (R)-3-(4-(2-(2-methyltetrazol-5-yl)-pyridin-5-yl)-3-fluorophenyl)-5-hydroxymethyl oxazolidin-2-one dihydrogen phosphate, methods of making the crystalline form and pharmaceutical compositions comprising the crystalline form are useful antibiotics. Further, the derivatives of the present invention may exert potent antibacterial activity versus various human and animal pathogens, including Gram-positive bacteria such as Staphylococi, Enterococci and Streptococi, anaerobic microorganisms such as Bacteroides and Clostridia, and acid-resistant microorganisms such as Mycobacterium tuberculosis and Mycobacterium avium. Accordingly, the compositions comprising the crystalline form may be used in antibiotics.

The invention belongs to the technical fields of environmental engineering and microbial engineering, and in particular relates to a neosphingobacterium strain that degrades polycyclic aromatic hydrocarbons at low temperature and its application in groundwater bioremediation of petroleum hydrocarbon polluted sites. The strain is Novosphingobium sp., which was deposited in the "General Microbiology Center of China Committee for the Collection of Microorganisms" on October 12, 2009, with the preservation number CGMCC No.3326. The bacterial strain of the present invention is isolated from a certain polluted site in Jilin Oilfield, and obtained through low-temperature enhanced natural enrichment and screening. It can degrade polycyclic aromatic hydrocarbon pollutants under low temperature conditions, and is especially suitable for bioremediation of groundwater in petroleum hydrocarbon-contaminated sites.

The invention discloses an efficient microbial soil remediation agent, belonging to a propagation culture technique of microbes. The efficient microbial soil remediation agent is prepared by mixing the following components in percentage by volume: 10-25% of photosynthetic bacteria zymogen solution, 25-35% of Bacillus subtilis zymogen solution, 20-35% of microzyme zymogen solution and 20-40% of actinomycetes zymogen solution. The four strains are respectively subjected two secondary amplification culture under the conditions of proper temperature, ventilation, culture medium, pH value and culture time; and the culture process is carried out in a fermentation tank without foreign microbes. By using the modern biotechnology, single microbe strains are subjected to high-concentration pure culture and then compounded to produce the efficient microbial soil remediation agent.

The invention belongs to the technical field of plant protection, and relates to a tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3. The bio-control strain is a bacillus subtilis. A method for preparing a microbial agent by using the strain Trb3 comprises the steps that: the strain Trb3 is subjected to oscillation culturing in an LB culture solution for 36-40h under a speed of 180rpm under temperature of 28 DEG C; the obtained material is centrifuged for 10min under a speed of 6000rpm; the material is diluted by using sterile water, such that the microbial agent is prepared, wherein a viable bacteria total concentration in the microbial agent finished product is 1*10<9>-1*10<10>u / ml. The Trb3 strain microbial agent is applied for root dipping before tobacco transplanting or for root irrigating during tobacco transplanting, and for root irrigating after tobacco transplanting and right before disease periods of tobacco black shank and tobacco bacterial wilt. In a greenhouse, a controlling effect of the Trb3 strain microbial agent against tobacco black shank reaches 72.3%, and a controlling effect of the Trb3 strain microbial agent against tobacco bacterial wilt reaches 65.7%. When the Trb3 strain microbial agent is applied in field, a controlling effect of the Trb3 strain microbial agent against black shank and tobacco bacterial wilt is 61.04%.

The invention relates to the technical field of soil conditioners and particularly relates to a soil heavy metal biological fixing agent and an application method thereof. The formula of the soil heavy metal biological fixing agent is that every one kilogram of an organic fertilizer is added with 2-10 g (109 CFU / g) of B. natto solid leavening agent, 2-10 g (109 CFU / g) of B.mucilaginosus solid leavening agent, 2-6 g (109 CFU / g) of B.megaterium solid leavening agent and 4-10 g (1010 CFU / g) of Trichoderma koningii solid leavening agent, wherein the main ingredients of the biological organic fertilizer contain more than 40% of organic matter, more than 20 million / g of effective viable count, and more than 5% of total nutrient. The soil heavy metal biological fixing agent is low in cost, has few environmental risks, remarkably reduces the absorption of the heavy metals of the crops, improves the soil fertility, effectively prevents and cures the soil-borne diseases and increases the economic benefit of the crops.

A biological product in the form of liquid or wettable powder for preventing and eliminating the bacterial soft rot of konjak is prepared from the Bacillus amyloliquefaciens C3 (CGMCC No.1553) through liquid fermenting to obtain the cultured substance, adding wetting agent, adhesive and additive, and preparing liquid or wettable powder.

The present invention provides a microbial fermented composite antibiotic-free feed containing natural antibacterial components. The feed comprises a fermented product based on the united segmented fermentation of aerobic-anaerobic composite strains, composite natural antibacterial components, a non-plant source protein / polypeptide compound nutrient supplement agent, and an additive comprising atleast a soybean protein amino acid / polypeptide compound chelate. The composite strains comprises at least lactic acid bacteria, bacillus subtilis, yeast selected from candida utilis or saccharomyces cerevisiae, and enterococcus faecalis. The fermented product based on the united segmented fermentation is prepared by carrying out secondary fermentation on fermentation raw materials through the aerobic-anaerobic composite strains. The combination of the microbial fermented feed and the natural antibacterial components is beneficial to the balance of intestinal flora of animals, and can prevent the dysbacteriosis of digestive tracts, improve the immunity functions of animal organisms, reduce or replace the use of feed antibiotics, and significantly improve the digestion and absorption efficiency of the feed and the meat quality of animals.

The invention belongs to the technical field of the microorganism, and in particular to a treatment method of soil contaminated by radioactive nuclide uranium or cesium. The invention adopts a bar screen, a double-plate screen and a spiral screen to screen the soil contaminated by uranium or cesium. The soil particles with the particle size more than 0.25mm are clean soil particles, the soil particles with the particle size less than 0.25mm are soil contaminated by uranium or cesium; and the soil particles with the particle size less than 0.25mm are mixed with active acidithiobacillus ferrooxidans for treatment, and the settlement is clean soil after filtration, the filtrate is transferred and mixed with active sulfate reducing bacteria for treatment, then the uranium or cesium is precipitated and can be recovered through filtering separation, and the water can also be recovered. The invention has the advantages of simple operation and low cost, and realizes the rapid and efficient removal of the uranium or cesium in the slightly contaminated soil.

The invention discloses a method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application, which belong to the technical field of microbial agents. In the invention, an initial strain is clostridium butyricunm JSIM-MCB20040312 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.1647; the butyric acid bacteria powder is prepared by the steps of activation of butyric acid bacteria seeds, microencapsulated embedding of the butyric acid bacteria seeds, viable counting and preparation of butyric acid bacteria powder, wherein the concentration of the obtained bacteria powder is not less than 1.0*1011 / g. The viable organism propagation capacity of the butyric acid bacteria subjected to microencapsulated embedding is enhanced; a strict anaerobic environment created by liquid sealing of nitrogen gas or carbon diode and other inert gases or paraffin is avoided; a high-concentration bacteroid can be obtained without introducing air into a general fermentation tank for culturing; the production cost is low and the requirement for fed microbial additives on viable count can be met; the butyric acid bacteria powder also has the characteristics of heat resistance, acid resistance and favorable entericsolubility and is suitably used as animal intestine probiotics; and intestinal diseases of livestock and poultry farming animals such as chicken, pigs and the like are effectively prevented and the growth of the bred animals is promoted.

The invention relates to a straw decomposing agent produced from raw materials of bacteria and fungi, and a production method thereof. Bacillus subtilis, saccharomyces cerevisiae, Aspergillus oryzae and aspergillus niger are assembled as composite bacteria and are individually cultured through steps of preparation of medium, control of an inoculation amount, a fermentation temperature and a fermentation time, and adjusting of a PH value parameter, concentration and drying. The bacteria are blended and proportioned to prepare into a decomposing agent capable of returning crop straws into field on the spot. The decomposing agent has advantages of strong pertinence, good decomposing effect and stable quality and performance.

The invention relates to a Bacillus amyloliquefaciens Y10 and application thereof. The Bacillus amyloliquefaciens Y10 is collected by China General Microbiological Culture Collection Center in China Committee for Culture Collection of Microorganisms on October 18th, 2013, and the collection number is CGMCC No.8362. The Bacillus amyloliquefaciens Y10 provided by the invention is separated from soil; the optimal growth temperature is 25-35 DEG C, and the pH value is 6.8-7.5; and the Bacillus amyloliquefaciens Y10 has the capacity for producing amylases, proteases and lipases. When being used for anaerobic fermentation of kitchen waste, the Bacillus amyloliquefaciens Y10 enhances the marsh gas production rate by 15-40%. The Bacillus amyloliquefaciens Y10 can effectively enhance the anaerobic fermentation hydrolysis and marsh gas production efficiency of waste or wastewater containing proteins, starch, fat and the like, and has important meanings for promoting conversion of organic waste into harmless substances, resources and energy sources.

The invention discloses biological, organic and inorganic compound fertilizer. The biological, organic and inorganic compound fertilizer comprises, by weight, 20-30 parts of lignin, 10-20 parts of organic fertilizer, 15-25 parts of nitrogen fertilizer, 30-40 parts of phosphorus fertilizer, 5-15 parts of potassium fertilizer, 0.1-0.2 part of EM (effective microorganisms) fungus powder, and 0.1-0.2 part of bacillus subtilis powder. Therefore, microenvironment of soil can be improved evidently, activity of beneficial microorganisms in soil is facilitated, and releasing of phosphorus in soil is facilitated.