881 results about "Bacillus <bacterium>" patented technology

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention discloses a microbial compound fertilizer for improving soil pollution, which is prepared by mixing a matrix fertilizer, alginic acid, cellulase, modified bentonite, biochar, a microbial agent, nano zero-valent iron and monopotassium phosphate according to a mass ratio of (30-40): (6-10): (5-8): (20-25): (15-20): (3-5): (4-6): (4-8), the microbial agent comprises pseudomonas, bacillus and silicate bacteria; heavy metal ions in soil can be effectively neutralized through the nanoscale zero-valent iron and the phosphate, pollutants in the soil can be effectively degraded through the microbial flora, meanwhile, nutrients needed by plant growth are provided, the modified bentonite is added, the soil structure can be changed, meanwhile, phosphorus and potassium fertilizers are provided for the soil, and the soil quality is improved. By adding alginic acid and cellulase, the activity of microorganisms is effectively enhanced, the fertility conversion efficiency of the microorganisms is improved, and soil improvement and remediation are achieved.

The invention belongs to the technical field of soil fertilizers, and particularly relates to a soil conditioner and a preparation method thereof. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 30 to 40 parts of modified charcoal, 20 to 30 parts of microbial complexing agent, 15 to 25 parts of humic acid and 1 to 5 parts of biological enzyme; according to the microbial complexing agent, dopamine grafted and modified nano silicon dioxide and hydroxyapatite form a composite shell, pseudomonas aeruginosa and bacillus subtilis are wrapped, and the microbial complexing agent has the functions of protecting microorganisms, adsorbing heavy metals and slowly releasing phosphorus elements; the modified biochar is compounded with the attapulgite through acid-base treatment to form a porous structure with physical inlaying and chemical functional groups synergistic, so that the adsorption and fixation capability on heavy metals and organic pollutants is remarkably improved; the biological enzyme accelerates organic phosphorus conversion, and enzymatic hydrolysate provides a phosphorus source for microorganisms, promotes microorganism breeding, effectively increases the content of available phosphorus in soil, and enhances the stability of a remediation system.

The invention belongs to the technical field of microorganisms, and discloses a low-temperature-resistant aerobic denitrifying bacterium and application thereof. The low-temperature-resistant aerobic denitrifying bacterium is Enterobacter ludwigii CW-01, and the preservation number of the low-temperature-resistant aerobic denitrifying bacterium is CGMCC (China General Microbiological Culture Collection Center) No.27126. The low-temperature-resistant aerobic denitrifying bacterium can be used for preparing the The enterobacter lewis CW-01 strain disclosed by the invention can efficiently realize aerobic denitrification degradation of ammonia nitrogen, nitrate and nitrite within the temperature range of 4-35 DEG C, and is especially suitable for wastewater treatment in which the ammonia nitrogen, nitrate and nitrite are difficult to remove due to low temperature; the strain also has good salt resistance, and provides a good microbial material for biological nitrogen removal treatment of low-temperature, high-salt and high-ammonia nitrogen polluted water. The nitrogen is efficiently removed, meanwhile, the use amount of a carbon source is remarkably reduced, the enterprise cost is greatly reduced, and the method has a good application prospect in the field of sewage treatment.

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention discloses a method for reducing saline-alkali water into modern seawater suitable for culturing various seafoods by using microorganisms, a special fish fertilizer for the method and application of a preparation method of the special fish fertilizer, and the method comprises the following specific steps: 1, pretreating the saline-alkali water: pumping saline-alkali with the salinity of 15-30% o into a culture pond, compressing, mixing and precipitating until the pH value is 7.3-7.5; 2, performing microbial culture: performing multi-stage enrichment on halobacterium violaceum and selecting dominant colonies; the method comprises the following steps: mixing underground saline-alkali water with the salinity of 10-20% o, saline-alkali water of a salt lake with the salinity of 180-200% o and high saline-alkali soil containing part of organic matters, cooking and sealing at the temperature of 200-210 DEG C, inoculating selected mixed halobacterium purple, pumping air, culturing with sunlight, and propagating for later use, wherein the pH value is 8.3-8.5; 3, reduction reaction: carrying out reduction reaction in a static water environment, when the water body is red brown or dark brown, inoculating a heterotrophic photosynthetic bacteria liquid and an autotrophic photosynthetic bacteria liquid with concentration, inoculating yeast powder, carrying out common reaction, then inoculating a special aquaculture fish fertilizer preparation, carrying out oxygenation reaction, and adjusting the pH value to 8.5-8.8; real seafood can be cultured in saline-alkali water in deserts and gobi deserts, zero pollution and zero emission can be achieved, the cost of development, implementation and popularization is low, and the cost of 10,000 cubic meters of water is only 675 yuan.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a strain of saline-alkali-resistant phosphorus-solubilizing compost bacillus subtilis C007, which can enhance the survival and colonization of beneficial microorganisms in saline-alkali soil farmland soil and plant root systems, also has the functions of solubilizing phosphorus and secreting auxin (IAA), and plays a role in promoting the growth of plants. The compound microbial fertilizer can improve the soil environment and the agricultural ecosystem on one hand, can promote the growth of plants, and can achieve the purposes of reducing the application of chemical fertilizers and improving the soil fertility of saline-alkali land farmlands to a certain extent.

The invention provides a composite microbial agent for decomposing insoluble phosphorus in soil and application thereof, and relates to the technical field of microorganisms, the active ingredients of the composite microbial agent comprise Achromobacter xylosoxidans, Burkholderia cepacia and Burkholderia parafungi, the Achromobacter xylosoxidans, the Burkholderia cepacia and the Burkholderia parafungi all have dissolving capacity for Ca3 (PO4) 2, FePO4, AlPO4, lecithin and calcium phytate, and the composite microbial agent can be used for decomposing insoluble phosphorus in soil. And the five insoluble phosphorus sources can be synergistically dissolved. The compound microbial agent is subjected to shake culture, the phosphate-solubilizing activity can reach 53.32 mg.L <-1 >, the phosphate-solubilizing function is stable, meanwhile, the compound microbial agent has the characteristics of degrading lignin and generating siderophore and indoleacetic acid, and the compound microbial agent has remarkable phosphate-solubilizing and growth-promoting effects when being applied to the vicinity of plant rhizosphere soil; the technical problem that in the prior art, the variety of phosphorus dissolving sources of phosphate solubilizing bacteria is single or the stability of the phosphate solubilizing function is poor is solved.

The invention discloses a method for promoting bacillus soil microorganisms to actively absorb oxygen to degrade long-chain alkanes, which comprises the following steps: preparing a catalyst: uniformly mixing oil-containing soil, a carbon source, citric acid, ferric salt, hydrogen peroxide and distilled water, filtering, concentrating clear liquid to obtain a concentrated crystal substance, centrifugally cleaning the crystal substance, and drying to obtain the catalyst; the method comprises the following steps: mixing a catalyst with oil-containing soil, hydrogen peroxide and distilled water, and oscillating on a shaking table for Fenton pre-oxidation; after pre-oxidation, intermittent oscillation oxygenation is carried out, bioremediation is carried out on the petroleum-contaminated soil at normal temperature, and the three petroleum bacteria in the soil are activated. Through pre-oxidation treatment, consumption of soluble organic carbon by microorganisms in soil is stimulated, the active oxygen uptake process of the microorganisms is accelerated, and the carbon metabolism process is accelerated. Finally, the bacillus-like microorganisms capable of degrading the petroleum are activated, so that the aim of degrading the petroleum hydrocarbon in the soil for a long time is fulfilled.

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

Methods are provided for enhancing the health, growth and / or sturdiness of turf grasses by applying a combination of microbes and / or their growth by-products to the turf grass roots and / or to soil. Specifically, in one embodiment, the subject invention utilizes Trichoderma harzianum and a strain of Bacillus amyloliquefaciens. The methods can further be used to improve maintenance and upkeep of natural turf grass fields used in, for example, athletics and recreation, as well as to increase the functionality and safety of these natural turf grass fields.

The invention relates to the technical field of new strains, and provides a new strain which is screened from Xinjiang saline-alkali soil, is classified and named as sphingomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.32220. Research finds that the strain can remarkably reduce the soil conductivity, the exchangeable sodium content, the alkalization degree and the total salt content, so that the soil quality is improved. The organic matter content and the acid phosphatase activity of the soil are remarkably improved, and the quality of the saline-alkali soil can be efficiently improved. Meanwhile, the invention provides a compound bacterial fertilizer for effectively improving saline-alkali soil, the compound bacterial fertilizer comprises bacillus subtilis, Exiguobacterium acetylbacter, Bacillus velezensis, Pseudomonas fluorescens, Rhodococcus capsulatus and a new strain Sphingomonas sp., and research finds that compared with a single bacterial solution, the compound bacterial solution has a more prominent remediation effect on saline-alkali soil.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention discloses a heavy metal pollution remediation material combining minerals and bacteria and a preparation and remediation method thereof. The heavy metal pollution remediation material comprises the following components in parts by mass: 50 parts of clay minerals, 30 parts of phosphate minerals, 10 parts of bacteria and the balance of organic fertilizer as a matrix, wherein the clay mineral is attapulgite, the phosphate mineral is apatite, and the bacteria are one of enterobacter. By combining the characteristics of minerals and bacteria, the remediation material has strong adsorption capacity and can effectively remove heavy metal ions in soil. Meanwhile, the enterobacter converts heavy metal ions into insoluble precipitates through a bioremediation mechanism of the enterobacter, such as adsorption, reduction, chelation and the like, so that subsequent treatment is facilitated. The metabolic activity of the enterobacter in the soil can promote the growth and activity of soil microorganisms, increase the content of beneficial elements in the soil, improve the soil structure and improve the soil quality.

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

A preparation method of a rice straw biochar loaded with Bacillus cereus, comprising: (1) washing, drying, grinding and sieving rice straws; (2) performing anaerobic pyrolysis treatment on the rice straws treated in the step (1) at 300-700° C. to obtain a biochar; (3) treating the biochar obtained in the step (2) with hydrochloric acid, then washing until a washing solution is neutral, drying, grinding, sieving and sterilizing to obtain a sterilized biochar; and (4) performing mixed culture on the sterilized biochar and a solution of Bacillus cereus subjected to activation culture, and then centrifuging after the end of culture to remove supernatant, so as to obtain a rice straw biochar loaded with Bacillus cereus having a carbon immobilizing capability.

The invention belongs to the technical field of probiotic fermentation, and discloses a preparation method and application of a bifidus yeast fermentation product, and the preparation method of the bifidus yeast fermentation product comprises the following steps: S1, preparing a medicinal phellinus igniarius extract A; s2, preparing a compound extract B of rhizoma anemarrhenae, radix puerariae, sophora flower buds and soapberry; s3, preparing a bifidobacterium fermentation product, namely inoculating the bifidobacterium seed solution into a fermentation culture medium, and culturing and fermenting under an anaerobic condition to obtain a primary fermentation liquid C; and adding A, B and a bifidobacterium seed solution into C, carrying out secondary fermentation, and carrying out post-treatment to obtain the bifidobacterium fermentation product. The obtained bifidus yeast fermentation product can reduce skin oxidative stress and inhibit inflammatory factors IL-6 and 5 alpha-reductase, is beneficial to skin absorption when externally applied, has a good transdermal effect on skin and an excellent oil control effect, can promote water-oil balance of skin, and achieves the technical effects of controlling oil, removing acnes and improving pores.

The invention relates to the field of environment, phosphorite resource utilization and agricultural biotechnology, in particular to a phosphate ore-containing phosphate-solubilizing bacterial agent, a preparation method thereof, a biological bacterial fertilizer and application, the phosphate ore-containing phosphate-solubilizing bacterial agent comprises phosphate-solubilizing bacteria, hydroxyapatite and a culture medium, and the phosphate-solubilizing bacteria are enterobacter hormaechei HS-6. The hydroxyapatite is used for preparing the phosphate ore-containing phosphate-solubilizing bacterial agent, so that the phosphate ore resource can be greatly promoted to be fully utilized, and the dissolution of insoluble phosphorus in the phosphate ore is realized. According to the phosphate-containing ore phosphate-solubilizing bacterial agent, the enterobacter hormaechei HS-6 is combined with the hydroxyapatite, so that the phosphate-solubilizing capacity of the enterobacter hormaechei HS-6 can be utilized, and the phosphorus resource of the hydroxyapatite can be fully utilized. The phosphate-containing ore phosphate-solubilizing bacterial agent has good prospects in application to preparation of biological bacterial fertilizer, application to agricultural soil improvement, application to phosphate ore resource recovery and application to promotion of wheat growth.

The invention discloses a psychrobacter haeli PN-Ta3 derived from turbot intestinal tracts and application of the psychrobacter haeli PN-Ta3, and belongs to the technical field of microorganisms. The psychrophilus haeli PN-Ta3 is separated from the intestinal tract of a healthy cultured turbot, and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, China, the preservation date is January 25, 2024, and the preservation number is CCTCC NO: M2024201. The psychrobacter nivalis PN-Ta3 disclosed by the invention has the beneficial effects that the psychrobacter nivalis PN-Ta3 has no pathogenicity to healthy turbots and has good colonization performance, and after the psychrobacter nivalis PN-Ta3 is prepared into freeze-dried bacterial powder and is mixed with and added to feed the turbots, not only can the growth and intestinal development of the cultured turbots be promoted, but also the intestinal microbial structure is optimized; the composition can also delay the morbidity and death of turbots caused by infection of aeromonas salmonicida BHAS-1.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses an extreme thermophilic charcoal complex microbial inoculant and application thereof in aerobic composting of pig manure, and belongs to the technical field of microorganisms. The invention firstly discloses a strain of thermophilic thermophilic bacteria N1209, and secondly discloses an extreme thermophilic biochar complex microbial inoculant, which comprises geobacillus thermoglucosidase BGSC 95A1, bacillus licheniformis ZR-1, the thermophilic thermophilic thermophilic bacteria N1209 and geobacillus stearothermophilus L5. The extremely thermophilic biochar complex microbial inoculant has the characteristic of high temperature resistance, compared with a single microbial inoculant, produced amylase, protease and lipase are higher in activity and show synergistic advantages, decomposition of pig manure can be remarkably promoted, the extremely thermophilic biochar complex microbial inoculant has advantages in the aspects of nitrogen element retention and CH4 and N2O emission reduction in the pig manure composting process, and the extremely thermophilic biochar complex microbial inoculant can be applied to the field of pig manure composting. And a new method is provided for treatment of livestock breeding wastes.

This invention discloses a method for remediating groundwater containing organic pollutants, comprising the following steps: 1) Extracting groundwater containing organic pollutants using multiple extraction pumps, adding ferrous salts at a solid-liquid ratio of 1:50-200, mixing thoroughly, and adjusting the pH of the system to 6-8 to obtain intermediate treatment solution one; 2) Adding a remediation agent to the obtained intermediate treatment solution one, mixing and stirring for 3-6 hours to obtain intermediate treatment solution two; 3) Passing the obtained intermediate treatment solution two sequentially into an anaerobic reactor and an aerobic tank for treatment, and discharging after reaching the required standards; the packing material of the anaerobic reactor contains microorganisms, including at least two or more of denitrifying bacteria, Bacillus coagulans, Bacillus subtilis, Bacillus clonorchis, Bacillus indusiata, and Serratia marcescens. The remediation method provided by this invention has high remediation efficiency, thorough removal of organic matter, and is economically feasible, with an organic matter removal rate of over 98%.

The invention relates to a domestication cultivation method and a high-density fermentation process of bifidobacterium longum, and the domestication cultivation method comprises the following steps: step 1, single colony separation: bifidobacterium longum is subjected to single colony separation, is cultured under an anaerobic condition until single colonies grow out, and is placed in an aerobic environment for several days; step 2, primary culture: during placement, selecting a plurality of grown single colonies every day, inoculating the single colonies into a dominant culture solution A for anaerobic culture to obtain a bacterial solution A, observing a bacterial turbid phenomenon or a bacterial precipitate generation condition in the bacterial solution, selecting the single colonies selected in the last day and the bacterial solution with the bacterial turbid phenomenon or the bacterial precipitate generation condition as industrial primary strains, and carrying out primary culture on the industrial primary strains; and preserving the bacterial liquid of the obtained industrial primary strain. After being domesticated and cultivated, the strain can be subjected to high-density fermentation under the aerobic condition by utilizing a pure plant nitrogen source, the fermentation condition is easy to control, the operation is simple, and the strain is suitable for industrial fermentation production.

The invention belongs to the technical field of wastewater treatment, and particularly relates to an arsenic wastewater treatment agent, a preparation method, application and an arsenic wastewater treatment method.The arsenic wastewater treatment agent comprises a carrier and a bacterial suspension adsorbed and fixed to the carrier; the carrier is iron modified biochar; the bacterial strain in the bacterial suspension is bacillus subtilis W7, and the bacterial strain in the bacterial suspension is bacillus subtilis W7. The bacterium-carbon composite material prepared through freeze drying can be compatible with multifunctional additives (such as iron oxide), can quickly activate the metabolic activity of strains through rehydration, and is suitable for groundwater remediation or industrial prefabricated remediation agent production with large temperature difference and high storage requirements.

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial composite flora with nitrogen removal capacity as well as a construction method and application of the microbial composite flora. The microbial composite flora with the nitrogen removal capability comprises mixed leissella luricola, leissella luricola, bacillus mucilaginosus, selaginella hydrocarbonoclada and rosinia aggresticus A S419 (Roseium aggresticus A S419), and the microbial composite flora with the nitrogen removal capability is prepared from mixed leissella luricola. The ratio of the viable counts of the mixed leptosticia parviflora to the viable counts of the leptosticia parviflora to the viable counts of the bacillus mucilaginosus to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the Research shows that the constructed microbial composite flora with the nitrogen removal capacity is a flora capable of efficiently removing nitrate nitrogen and nitrite nitrogen in high-salinity water in mariculture and the like, and a simple and effective operation method is provided for nitrogen removal of high-salinity wastewater.

The invention is applicable to the technical field of soil remediation, and particularly relates to a method for enhancing phytoremediation of heavy metal cadmium contaminated soil, which comprises the following steps: planting hyperaccumulators in to-be-remediated heavy metal cadmium contaminated soil, and regularly applying bacterial liquid to the soil at the roots of the hyperaccumulators in the growth cycle of the hyperaccumulators. According to the method, inoculation of the thiobacillus ferrooxidans in the soil has no influence on the soil environment, growth of hyperaccumulators and growth, yield and quality of crops, the price is low, and large-area popularization and application can be achieved in practical application.