476 results about "Bacillus <bacterium>" patented technology

The invention belongs to the technical field of soil fertilizers, and particularly relates to a soil conditioner and a preparation method thereof. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 30 to 40 parts of modified charcoal, 20 to 30 parts of microbial complexing agent, 15 to 25 parts of humic acid and 1 to 5 parts of biological enzyme; according to the microbial complexing agent, dopamine grafted and modified nano silicon dioxide and hydroxyapatite form a composite shell, pseudomonas aeruginosa and bacillus subtilis are wrapped, and the microbial complexing agent has the functions of protecting microorganisms, adsorbing heavy metals and slowly releasing phosphorus elements; the modified biochar is compounded with the attapulgite through acid-base treatment to form a porous structure with physical inlaying and chemical functional groups synergistic, so that the adsorption and fixation capability on heavy metals and organic pollutants is remarkably improved; the biological enzyme accelerates organic phosphorus conversion, and enzymatic hydrolysate provides a phosphorus source for microorganisms, promotes microorganism breeding, effectively increases the content of available phosphorus in soil, and enhances the stability of a remediation system.

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention relates to the technical field of compound microbial fertilizers, in particular to a biochar-based compound microbial fertilizer for improving saline-alkali soil. The organic fertilizer comprises the following components in parts by weight: 30-50 parts of straws, 10-15 parts of compound microbial capsules, 20-40 parts of compost and 5-10 parts of humic acid. A multi-strain mixture composed of azotobacter chroococcum, pseudomonas and bacillus mucilaginosus is added, and comprehensive improvement of the saline-alkali soil is achieved; the azotobacter chroococcum can convert nitrogen in the air into nitrogen which can be utilized by plants, so that the dependence on chemical fertilizers is reduced; pseudomonas has efficient phosphate solubilizing capacity, and bacillus mucilaginosus can strongly solubilize potassium, so that the soil fertility is comprehensively improved. Composite microorganisms and polylactic acid are subjected to an interfacial polymerization reaction to prepare microcapsules, dopamine hydrochloride is utilized to form a coating on the surfaces of the microcapsules in a self-assembly mode, physical protection can be provided for the microorganisms, the survival rate of the microorganisms in a severe environment is increased, and the acting time of the microorganisms in soil is prolonged.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The invention provides a microbial agent capable of degrading lignin and application of the microbial agent in regulation and control of a soil organic carbon library, and relates to the technical field of microorganisms, the microbial agent contains at least two of serratia marcescens, ochrobactrum anthropi, pseudomonas asparagi or burkholderia terricola, and the microbial agent contains at least two of the serratia marcescens, the ochrobactrum anthropi, the pseudomonas asparagi and the burkholderia terricola. The serratia marcescens a26, the ochrobactrum anthropi b1, the pseudomonas asparagi b6 or the burkholderia terricola b21 have good lignin degradation capability, after compounding, the lignin degradation capability of the strain is further improved, and the strain can be used for degrading moso bamboo litters with high lignin content and promoting accumulation of a soil organic carbon library. The technical problem that a microbial agent with a function of efficiently degrading lignin is lacked in the prior art is solved.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.

The invention relates to the field of agricultural planting, in particular to a post-ginseng land soil improvement method based on compound bacteria fermentation degradation of allelochemicals. The invention provides a main technological process for improving soil through a fermentation method. The main technological process comprises the steps of land consolidation, ridge forming and pond building, material preparing and feeding and soil-material mixing, zymophyte inoculation and bacterium-material combination, film mulching anaerobic and fermentation monitoring, fermentation completion and post-treatment. The invention also provides a complex microbial inoculant composition capable of achieving the improvement effect, and the complex microbial inoculant composition comprises the following components in parts by weight: 5 parts of straw returning agent, 3 parts of organic fertilizer leavening agent and 1 part of bacillus subtilis agent. According to the method, the improved soil is subjected to long-time anaerobic fermentation in the water stain micro-ecological environment, the organic matter content of the soil can be greatly increased by adding a large number of organic matter materials, the carbon / nitrogen ratio of the soil is increased, the bulk density of the soil is reduced, fertility is comprehensive, sufficient and balanced, and the method is suitable for continuous cropping planting and healthy growth of ginseng.

The invention relates to a specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof, and belongs to the technical field of microbiological detection. Every 1000 mL of the specific culture medium contains 5-25 g of a nitrogen source, 5-10 g of a carbon source, 0.02-16 g of an inhibitor, 0.01-0.02 g of a color indicator, 0.1-13 g of a conditioning agent and the balance of a solvent. The inhibitor comprises Tween 80, a tea tree oil extract, cholate, sodium laurate and hydroxyproline. The specific culture medium provided by the invention is a propionibacterium acnes selective culture medium which is free of antibiotics, high in selectivity, sensitive in color development and low in cost. By optimizing the components of the culture medium and adding the selective inhibitor and the color indicator, the growth of other facultative anaerobic bacteria such as lactic acid bacteria can be effectively inhibited, specific culture and color development detection of propionibacterium acnes are carried out, and the accuracy and reliability of detection are improved.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

The invention relates to the technical field of soil improvement and microorganisms, and discloses a preparation process of a porous activated carbon soil conditioner loaded with microbial flora, which comprises the following steps: firstly, performing high-temperature activation and acid-base modification on a biomass raw material to prepare a porous activated carbon carrier, and dipping in a nutrient solution to preset a slow-release nutrient substrate; secondly, adopting a step-by-step sequential loading process, firstly loading the bacillus subtilis subjected to stress resistance domestication on a carrier through electrostatic adsorption, and performing activation colonization culture to form a stable pioneer biological membrane; then, on the basis of the biological membrane, phosphate solubilizing bacteria, potassium solubilizing bacteria and antagonistic bacteria are loaded in sequence; finally, the loaded composite carrier is subjected to microcapsule embedding and low-temperature drying treatment, and the porous activated carbon soil conditioner is obtained. According to the modifier prepared by the invention, through ordered loading and multiple protection, the problem of strain competition is solved, the high survival rate of flora is ensured, and the improvement effect is stable and long-acting.

The invention discloses a carbon-based electrode-microorganism self-assembled compound, a preparation method thereof and application of the carbon-based electrode-microorganism self-assembled compound in strengthening anaerobic fermentation of agricultural wastes. The compound is formed by self-assembly of direct interspecific electron transfer (DITE) functional microorganisms (anode reduction geobacter and methanobacterium flexural) on the surface of a high-conductivity carbon-based electrode under the induction of a micro electric field, the relative abundance of the anode reduction geobacter is 65-82%, and the relative abundance of the methanobacterium flexural is 18-35%. During preparation, the carbon-based electrode is cleaned by acetone, ethanol and water, then the carbon-based electrode, a reference electrode and a counter electrode form a three-electrode system, and the three-electrode system is induced for 3-5 days in a micro electric field of-0.2-0.2 V to form a compound. 1-5 g / L of the compound is added into a straw-excrement mixed substrate (25-40 g VS / L) subjected to alkali pretreatment, anaerobic fermentation is conducted for 50 d after biogas slurry is inoculated, the pH can be stably maintained at 7.2-8.0, the accumulated biogas yield reaches up to 450-630 mL / g VS, and the volume fraction of methane is 68-82%. The process is simple, the compound is high and stable in activity, and efficient resource utilization of agricultural waste can be achieved.

The present disclosure relates to microbiome compositions for improving muscle strength using a heat-treated fermentative culture complex of KM2 Phytobacterium plantarum. The composition according to the present disclosure increases the synthesis of myoprotein and exhibits an effect of inhibiting the degradation of myoprotein. Animal tests show effects of improving muscle strength and increasing leg muscle mass by increasing grip strength, travel distance and speed, and results of improving popliteal muscle strength and increasing muscle mass even in clinical tests. Furthermore, it has been found that beneficial bacteria are increased by an increase in the diversity of the gut microbial flora, which varies as muscle decreases, and an improvement in the microbial flora composition, while harmful bacteria are reduced. Therefore, the composition can be used as a composition for preventing, alleviating or treating muscle diseases and a composition for improving muscle strength or increasing muscle mass.

The invention provides a compound microbial agent and application thereof in regulating aquaculture water quality, and the compound microbial agent is prepared by the following steps: firstly, embedding a bacterial liquid composed of alcaligenes faecalis, pseudomonas, ochrobactrum and sphingosine box bacteria through self-assembly by adopting gallic acyl quinic acid, and then modifying lignin by using ferrocene for composite loading. And immersing into a bacterial liquid consisting of photosynthetic bacteria, bacillus and nitrifying bacteria, and drying. According to the invention, ferrocene modified lignin and galloylquinic acid construct a multi-layer load structure to fix microorganisms in a layered manner, so that the microorganisms can stably survive in a complex water body environment, and meanwhile, a plurality of florae act synergistically to degrade organic matters, nitrogen, phosphorus, antibiotics and the like in aquaculture water, so that the degradation efficiency of water pollutants can be remarkably improved; effective adjustment of aquaculture water quality is facilitated, and good data support and theoretical basis are provided for water body improvement in the field of aquatic products.

The invention relates to the technical field of agricultural biological prevention and control, and discloses a biological prevention and control complex microbial inoculant and application thereof.The biological prevention and control complex microbial inoculant is prepared by the steps that natural bio-based compounds vanillin and vanillic acid serve as main raw materials, and a biodegradable trialdehyde monomer is designed and synthesized; a biodegradable trialdehyde monomer and p-phenylenediamine are used as organic building units to assemble and form an ordered porous crystal structure, and the biodegradable COF material is obtained; the preparation method comprises the following steps: loading a basic complex microbial inoculant consisting of bacillus thuringiensis, bacillus subtilis and streptomyces willerigensis on a biodegradable COF material by adopting an impregnation adsorption method, and coating the surface of the biodegradable COF material with tetraethylenepentamine and tannic acid through copolymerization to form a degradable polytannic acid layer, so as to prepare the slow-release complex microbial inoculant, and the effective active components of the slow-release complex microbial inoculant can be slowly released; the lasting period and the utilization rate of the composite microbial agent can be remarkably improved, and the composite microbial agent shows an obvious inhibition effect on pathogenic bacteria such as fusarium oxysporum and botrytis cinerea and can be applied to prevention and treatment of plant diseases.

The present invention relates to means and methods comprising a bacterial heme peroxidase and / or a functional fragment thereof for medical and non-medical use in preventing and / or controlling a pathogen on food, plants, or in or on the human or animal body. The present invention thus relates to novel medical and non-medical uses of a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants, or on the human or animal body. Furthermore, the present invention relates to corresponding methods comprising a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants or on or in the human or animal body comprising applying a sufficient amount of the bacterial heme peroxidase and / or a functional fragment thereof on said food, plants, or on mucosae, skin, in particular epidermal skin like scalp or facial skin and / or teeth to reduce the number of cells of said pathogen. In addition, the invention relates to specific heme peroxidases that have been identified to have desired properties. The invention also relates to fragments of or small heme peroxidases that have particularly advantageous properties. Moreover, the present invention further relates to corresponding compositions comprising a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants, or in or on the human or animal body. Kits comprising components used in the methods and compositions of the invention are also provided herein. Furthermore, provided is the use of a bacterial heme peroxidase and / or of a functional fragment thereof for the preparation of the compositions of the present invention. The present invention finally also relates to a method for producing a bacterial heme peroxidase protein and / or a functional fragment thereof in an engineered Bacillus subtilis strain. The inventive use of the bacterial heme peroxidase of the present invention and / or a functional fragment thereof for preventing and / or controlling a pathogen is, without being limiting, particularly useful in ecologically compatible commercial plant / crop protection campaigns as well as in daily personal care products such as toothpaste for example.

The invention discloses a fertilizer for degrading phosphorus element residues in soil and improving the utilization rate of the fertilizer and a preparation method of the fertilizer. The invention belongs to the technical field of fertilizers, and through dipping treatment and high-temperature calcination of rice husk charcoal and a monopotassium phosphate solution, the surface of the prepared modified charcoal is rich in active functional groups, and the modified charcoal can adsorb and activate insoluble phosphorus in soil, improve the soil structure and enhance the permeability and water retention capacity of the soil at the same time; meanwhile, the phosphate-solubilizing bacillus, the pseudomonas and the arbuscular mycorrhizal fungi are compounded for use, so that the release efficiency of fixed-state phosphorus in the soil can be remarkably improved, and absorption and utilization of phosphorus by crops are promoted; citric acid, malic acid and tartaric acid are compounded for use and can form a stable chelate with metal ions in soil, the fixing effect of the metal ions on phosphorus is reduced, and the effectiveness of phosphorus is further improved; through reasonable trace element proportioning, the fertilizer can meet the requirements of crops on micronutrient elements in the growth process and promote healthy growth of the crops.

The invention discloses a synthetic microbial agent for treating uranium-contaminated soil and a preparation method of the synthetic microbial agent. The synthetic microbial agent is prepared from the following bacteria: P.adami, P.megatherium, bacillus subtilis, Artobacter volluticus, symbiotic fungi, Aspergillus niger, sulfate reducing bacteria and Shewanella. The symbiotic fungi are mucor lastein, mucor unimingguminum, mucor circinelloides, mucor dense, rhizopus arrhizus and phylum phthochytrium parasitic fungi. The prepared synthetic microbial agent and synthetic microbial embedded balls are applied to uranium-contaminated soil remediation, the key enzyme activity can be effectively improved, the soil metabolism function is enhanced, meanwhile, exchangeable uranium with high ecological risk is converted into stable and low-harm residual uranium, the mobility and bioavailability of uranium in soil are effectively weakened, and the uranium-contaminated soil remediation effect is improved. Therefore, immobilized remediation of uranium is realized, and a good soil uranium fixing effect is achieved.

There is provided a composition that comprises a propionic acid bacterium or a composition that comprises an oligosaccharide. The composition that comprises a propionic acid bacterium or the composition that comprises an oligosaccharide can suppress an increase of body weight of a subject by having the subject ingest the composition.

The invention discloses a method for carrying out soil treatment by using ardealite and a microbial agent, and aims to solve the problem that ardealite as an industrial by-product is difficult to recycle. According to the method, 5% of a microbial agent (one of trichoderma harzianum, arbuscular mycorrhizal fungi or bacillus laterosporus) and 5%-15% of biochar are applied to phosphogypsum in a combined mode, the nutrient content of a phosphogypsum matrix is remarkably increased, and meanwhile specific pollutants (such as soluble phosphorus, fluorine and heavy metal) are stabilized and solidified. Experimental results show that the technology can effectively regulate and control the pH value of the phosphogypsum matrix, obviously increase the diversity of effective microbial communities and improve the micro-ecological environment of the phosphogypsum matrix. The ardealite combined with the microbial agent and the biochar can be used as a plant growth matrix or a soil substitute material for ecological restoration of mines, has the characteristics of low cost and high efficiency, and provides a feasible way for harmlessness and soil utilization of the ardealite.

The present invention belongs to the field of microbial treatment technology, and specifically relates to a strain of Enterobacter hollandii and its application, which has the ability to efficiently dissolve Fe from iron tailings. 2+ / 3+ , Ca 2+ Mg 2+ The functional strain is used to treat coastal saline soil. The dissolved free metal ions can accurately target the Na + , by virtue of ion exchange, Na + Desorption and discharge of cultivated soil from saline soil bodies can effectively improve the high salinity and single composition problems of coastal saline soil, and provide advanced and reliable technical means for the ecological restoration and agricultural utilization of coastal saline soil.

The application discloses a high-salt-tolerant protease-producing Arthrobacter protophormiae and application thereof, and belongs to the technical field of environmental microorganisms.The application provides the Arthrobacter protophormiae A1 which is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M 20241041.The Arthrobacter protophormiae provided by the application can tolerate 8.0% salinity, and can effectively secrete protease under high-salt stress.The bacteria can provide effective strain resources for the anaerobic biological treatment of reinforced kitchen waste and other high-salt high-protein waste water, thereby significantly reducing the treatment cost, and have important application value.

The invention provides a novel ecological feed capable of preventing multiple grass-bream diseases, the novel ecological feed comprises a feed matrix and an ecological feed leavening agent, and the ecological feed leavening agent comprises probiotics with surface expression antigens, the antigen is any one or more of a grass carp reovirus antigen, a flavobacterium johnsonii antigen, an aeromonas veronii antigen and a clonorchis sinensis antigen. The novel ecological feed with a disease-resistant function in a parabramis pekinensis breeding mode is prepared for the first time, the resistance of the parabramis pekinensis to various pathogens can be enhanced, and the survival rate of the parabramis pekinensis in the disease attack peak period can be increased by 50% or above after immunization. An oral immunization mode is adopted and can be completed in the feeding process, and the immunization and breeding cost is greatly saved.

The invention discloses thermoanaerobacter sp SA-1, and the preservation number of the thermoanaerobacter sp SA-1 is CGMCC (China General Microbiological Culture Collection Center) No.28132. The invention further discloses a preparation method of the thermoanaerobacter sp SA-1. The thermophilic anaerobacter SA-1 has the beneficial effects that (1) the thermophilic anaerobacter SA-1 provided by the invention has the growth temperature of 60-85 DEG C, can quickly grow and breed under the anaerobic condition of medium-high-temperature oil reservoirs, metabolizes heavy components of heavy oil in a hydrocarbon-philic manner, generates a biological surfactant, reduces the oil-water interfacial tension to 28-35mN / m, improves the fluidity of the heavy oil, improves the recovery efficiency, and reduces the production cost; the recovery ratio is increased by more than 25% through a physical simulation experiment; (2) the fermentation liquor of the thermoanaerobacter SA-1 is applied to microorganism single well huff and puff, the average single well oil increasing amount is more than 1000t, and the validity period is more than 300d; and (3) the fermentation liquor of the thermoanaerobacter SA-1 is applied to microbial well group oil displacement, and the average daily oil increase of a well group is more than 10t.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.