303 results about "Bacillus <bacterium>" patented technology

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.

The invention discloses a synthetic microbial agent for treating uranium-contaminated soil and a preparation method of the synthetic microbial agent. The synthetic microbial agent is prepared from the following bacteria: P.adami, P.megatherium, bacillus subtilis, Artobacter volluticus, symbiotic fungi, Aspergillus niger, sulfate reducing bacteria and Shewanella. The symbiotic fungi are mucor lastein, mucor unimingguminum, mucor circinelloides, mucor dense, rhizopus arrhizus and phylum phthochytrium parasitic fungi. The prepared synthetic microbial agent and synthetic microbial embedded balls are applied to uranium-contaminated soil remediation, the key enzyme activity can be effectively improved, the soil metabolism function is enhanced, meanwhile, exchangeable uranium with high ecological risk is converted into stable and low-harm residual uranium, the mobility and bioavailability of uranium in soil are effectively weakened, and the uranium-contaminated soil remediation effect is improved. Therefore, immobilized remediation of uranium is realized, and a good soil uranium fixing effect is achieved.

The invention provides a novel ecological feed capable of preventing multiple grass-bream diseases, the novel ecological feed comprises a feed matrix and an ecological feed leavening agent, and the ecological feed leavening agent comprises probiotics with surface expression antigens, the antigen is any one or more of a grass carp reovirus antigen, a flavobacterium johnsonii antigen, an aeromonas veronii antigen and a clonorchis sinensis antigen. The novel ecological feed with a disease-resistant function in a parabramis pekinensis breeding mode is prepared for the first time, the resistance of the parabramis pekinensis to various pathogens can be enhanced, and the survival rate of the parabramis pekinensis in the disease attack peak period can be increased by 50% or above after immunization. An oral immunization mode is adopted and can be completed in the feeding process, and the immunization and breeding cost is greatly saved.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

This invention discloses a halophilic fast-growing bacillus strain that degrades chlorinated persistent organic pollutants and its applications. The strain is *Celeribacter baekdonensis* TBC005, with accession number GDMCC No:65236. The *Celeribacter baekdonensis* TBC005 of this invention exhibits excellent degradation ability against chlorinated persistent organic pollutants. After 14 days of anaerobic culture, it shows significant degradation of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP reached 21.5%, 16.7%, and 16.9%, respectively; after 35 days of cultivation, the degradation rates of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP can reach 50.7%, 59.2%, and 61.3%, respectively, and they can be applied to the environmental pollution control and bioremediation of chlorinated persistent organic pollutants.

The present application relates to the field of microbial technology, and specifically discloses a strain of Brachybacterium paraconglomeratum 2-1 and its application in industrial wastewater treatment; the Brachybacterium paraconglomeratum 2-1 is preserved in the China General Microbiological Culture Collection Center on May 29, 2025, with a preservation number of CGMCC No.34719 and a classification name of Brachybacterium paraconglomeratum; the strain 2-1 can tolerate the high toxicity environment of residual ammonia water in a coking plant, has a good effect in the microbial treatment of industrial wastewater containing phenol, can grow by taking phenol as the only carbon source, realizes the removal of phenol in wastewater under aerobic conditions, and has great application potential in the biological treatment of industrial wastewater.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The invention discloses a method for improving the processing quality of tobacco leaves by using a facultative anaerobic bacterium compound preparation, which comprises the following steps: preparing seed solutions of bacillus altitudinis ZY02-1, bacillus subtilis SMXP-58 and klebsiella variicola HNYJ-3, preparing a tobacco extracting solution, compounding the seed solutions according to the ratio of 2: 1: 2, centrifuging, resuspending and culturing by using the tobacco extracting solution to obtain a compound bacterial agent, and preparing the compound bacterial agent by using the facultative anaerobic bacterium compound preparation. And finally, spraying the complex microbial inoculant according to the inoculum size of 2-5% until the primarily-cured tobacco leaves are dampened until the moisture content is 14-15%, sealing, stacking and fermenting at 20-30 DEG C for 3-5 days, and then re-drying. By means of the method, decomposition of polyphenol and terpene aroma precursors of the tobacco leaves can be accelerated, the content of aroma substances such as megastigmatrienone is remarkably increased, aroma components such as oxidized isophorone are added, the aroma quality and the aroma amount of cigarettes are improved, offensive odor is reduced, and the aftertaste and smoking comfort are improved.

The present application relates to the technical field of fermented feed, and particularly relates to a compound feed based on aerobic-anaerobic combined fermentation, which comprises the following components in percentage by weight: fermented bacterial solution: 2-4%; Chinese herbal medicine additive: 0.5-1%; fermented base material: the balance; the preparation method of the compound feed comprises the following steps: S1, uniformly mixing the fermented bacterial solution and the fermented base material to obtain fermented material; S2, alternately performing aerobic-anaerobic combined fermentation according to the temperature of the fermented material to obtain pre-fermented material; S3, adding the Chinese herbal medicine additive, and alternately performing aerobic-anaerobic combined fermentation according to the temperature of the pre-fermented material to obtain the compound feed; the present application first promotes the proliferation of bacillus in a low-oxygen environment, decomposes macromolecular fibers and proteins, and accumulates metabolic products such as lactic acid, and then increases the ventilation frequency after heating to accelerate the release of active ingredients of the Chinese herbal medicine and improve the immunity of livestock and poultry.

One bacterial strain, designated HC1M1C21T, was isolated from human faeces and analyzed using phylogenetic, morphologic and biochemical approaches. Phylogenetic analyses based on 16S rRNA sequences and a set of 92 bacterial core genes indicated that this strain belongs to the Lachnospiraceae family and clusters near Pilosibacter fragilis (96.9% 16S rRNA sequence identity). HC1M1C21T has a DNA G+C content of 48.7 mol%. This strain is anaerobic, Gram- stain-positive, non-motile and non-spore-forming. HC1M1C21T cells appear as single rods or chained rods with tapered ends. Optimal growth was observed at 37°C, at pH between 5.5 and 6.5 and at salinity below 10 g / L. HC1M1C21T is a potent butyrate producer. The invention pertains to a new bacterial strain that produces butyrate and several independent factors with anti-inflammatory properties.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a composite solid phosphorus-solubilizing bacterial agent for repairing cadmium-polluted soil and improving soil quality, wherein the composite solid phosphorus-solubilizing bacterial agent comprises phosphorus-solubilizing bacteria, bone charcoal, culture medium and organic matrix; the phosphorus-solubilizing bacteria are Pantoea agglomerans CT-3 and Enterobacter hormaechei HS-6; the composite solid phosphorus-solubilizing bacterial agent is applied to Cd-polluted soil, and the biological availability of Cd in the soil is reduced by Pantoea agglomerans CT-3, Enterobacter hormaechei HS-6 and micron-nanometer multi-stage bone charcoal, and meanwhile, the soil quality is improved. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of effective soil phosphorus is increased by 98.19%; on the other hand, the application can fix soil heavy metal Cd, so that the content of effective Cd in the soil is reduced by about 52.50%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in repairing farmland Cd pollution, improving soil quality and environmental protection.

The invention belongs to the technical field of soil conditioners and fertilizers, and particularly relates to a bio-organic fertilizer for improving salt and alkali as well as a preparation method and application of the bio-organic fertilizer. The method comprises the following preparation steps: (1) carrying out pyrolysis treatment on dry agricultural and forestry wastes or livestock and poultry manure under anaerobic and 400-700 DEG C conditions, and crushing to obtain a biochar material; and (2) mixing and grinding the obtained biochar material, soybean meal and water, and then inoculating a bacillus subtilis activation solution and an actinomycetes activation solution for fermentation treatment to obtain the saline-alkaline improved bio-organic fertilizer. According to the bio-organic fertilizer, the biochar prepared from agricultural and forestry waste or animal dung is used as a main raw material, EPS generated through in-situ loading microbial fermentation metabolism can synergistically improve the improvement effect of the obtained organic fertilizer on saline-alkali soil and the passivation effect on heavy metal, absorption of crops on the heavy metal is remarkably reduced, and the fertilizer efficiency is improved.

The invention provides a soil remediation agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The soil remediation agent disclosed by the invention is prepared from 15 to 20 parts of a microbial agent, 20 to 25 parts of humic acid, 15 to 20 parts of fulvic acid, 5 to 10 parts of a soybean meal extract, 1 to 1.5 parts of a seaweed extract and 20 to 25 parts of a plant extract, wherein the microbial agent comprises an inner core composed of straw biochar and a compound bacteria solution, and a shell formed by methoxypolyethylene glycol modified chitosan, sodium caseinate and sodium tripolyphosphate; the composite bacterial liquid comprises a bacillus thuringiensis bacterial liquid and a beauveria bassiana bacterial liquid in a volume ratio of 10: (5-8). The soil remediation agent can improve the soil structure, relieve acidification and hardening, effectively resist diseases for a long time, fundamentally improve the physicochemical properties and micro-ecological environment of the ginger continuous cropping soil, and realize comprehensive remediation.

The application provides a kind of biological fertilizer prepared based on blue-green algae mud, and belongs to the technical field of biological fertilizer preparation.The biological fertilizer according to the application comprises, by weight fraction, 20-40 parts of blue-green algae mud fermentation liquor, 30-40 parts of straw, 18-36 parts of animal manure, 5-8 parts of urea, 6-12 parts of ammonium chloride and 0.2-1 part of yeast, wherein the blue-green algae mud fermentation liquor is prepared by mixing blue-green algae mud and heat condensate, then adding enzyme preparation, mixed Bacillus and inorganic salt mixture, and anaerobic fermentation culture.The application not only realizes the resource treatment of blue-green algae mud and reduces environmental pollution, but also the prepared biological fertilizer contains nutrients such as nitrogen, phosphorus and potassium required for plant growth, and the crops applying the biological fertilizer prepared by the application can not only improve the survival rate, but also further improve the yield per mu, improve the soil quality, and the soil porosity reaches 60-65%, without caking and residue.

The disclosure relates to methods for decreasing feed conversion ratios in fowl through administering isolated microorganisms to the fowl. In particular aspects, the disclosure provides methods of decreasing feed conversion ratios in fowl through administration of a Bacillus sp. to the fowl. The disclosure further relates to isolated microorganisms and compositions comprising the same.

The application provides a kind of high biocompatibility biological silver nanoparticles, its preparation method, compound microcapsule and application, the biological silver nanoparticles are based on the fermentation broth or fermentation filtrate of biocontrol bacillus B268, the metabolite of B268 is combined on the surface of particle, the antagonistic activity to plant pathogenic bacteria is enhanced, and show high biocompatibility to biocontrol bacteria, biocontrol fungi, plant, model nematode, can be directly embedded with bacteria liquid, the microcapsule prepared fuses the compatible interaction relationship between bacteria, bacterial metabolite, silver nanoparticles, shows synergistic antibacterial effect, and the bacteriostatic activity is higher than that of microcapsule embedded by bacteria liquid or silver nanoparticles alone, and under low temperature preservation condition, microcapsule can keep the stability of bacteria, silver nanoparticles and its synergistic antibacterial activity, provides preparation raw material and preparation method for developing nano biological pesticide.

The invention belongs to the technical field of soil improvement, particularly relates to a compound microorganism saline-alkali soil conditioner as well as a preparation method and application thereof, and aims at solving the problems that the existing soil conditioner mostly adopts a single strain or a simple combination, is difficult to colonize in a harsh saline-alkali environment, has a single function, cannot systematically improve the physical and chemical properties of soil, is slow in organic material decomposition and is difficult to promote. In order to solve the problems that in the prior art, the soil conditioner is not durable in effect due to the fact that the soil conditioner is prone to failure due to saline-alkali stress, the following scheme is provided: the soil conditioner comprises the following raw materials in parts by weight: 5-10 parts of bacillus; by using bacillus, streptomyces, saccharomycetes, actinomycetes, nitrogen-fixing bacteria, phosphate bacteria, potassium bacteria, pyroligneous liquor, seaweed extract, amino acid chelate, potassium fulvate, citric acid and monopotassium phosphate, the content of organic matters in soil can be effectively increased, the aggregate structure of the soil is improved, a more lasting soil improvement effect is achieved, and the soil conditioner has a good application prospect. And the use frequency and cost of the modifier are reduced.

The invention relates to a cyanide degrading bacterium, a culture application thereof and a preparation containing the cyanide degrading bacterium, the cyanide degrading bacterium is bacillus cereus 2H-2, the bacterium is preserved in the China General Microbiological Culture Collection Center on March 18, 2024, and the preservation number is CGMCC No.30063. The cyanide degrading bacterium has the advantages that the cyanide degrading bacterium can be used for degrading cyanide, and the cyanide degrading bacterium can be used for degrading cyanide; the strain provided by the invention can stably degrade cyanide in a water body with heavy metals, has good settleability and is easy to separate and obtain.

The application discloses a soil enterobacter capable of efficiently degrading polypropylene and application thereof. The strain is FJNUIM-S2, which is classified and named as soil enterobacter (Enterobacter agglomerans) Enterobacter soli ), is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is preserved on October 17, 2024, with a preservation number of CGMCC No. 32241 and a preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Xiliujiaobei, Chaoyang District, Beijing. The strain FJNUIM-S2 of the application can grow with polypropylene as the only carbon source, has good polypropylene degradation characteristics, provides a microbial resource for bioremediation of waste polypropylene in the environment, and has a wide application prospect.

The application belongs to the technical field of bioengineering and one-carbon resource utilization, and particularly relates to a strain and application of the strain in acetic acid and acetate production. Acetobacterium woodii The strain is Acetobacterium woodii (Acetobacterium woodii) SL70, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC No: 67680 and a preservation date of January 15, 2026. The strain can realize efficient conversion of H2 / CO2 in synthesis gas, and the CO2 utilization rate reaches 1.043 g / L / h. The fermentation process is carried out under anaerobic conditions and no pollutants are discharged, so that resource utilization of industrial tail gas and carbon emission reduction can be realized, and a new technical path for one-carbon resource conversion is provided.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

The invention relates to the technical field of microbial preparations for agricultural environment restoration, and provides a microbial agent for reducing pesticide residues in soil, which is characterized by comprising the following components in parts by weight: 30-60 parts of ochrobactrum having viable count of more than or equal to 5 * 10 < 8 > cfu / g; the spore number is greater than or equal to 2 * 10 < 8 > cfu / g. The final residues of imidacloprid and difenoconazole in the compounded microbial agent are reduced to about 0.20 mg / kg, balanced and efficient removal is realized, and the possible unbalance situation of'degrading one pesticide and remaining one pesticide 'in the traditional method is avoided.

The invention provides a biochemical composite soil heavy metal pollution fixing and repairing material and a preparation method thereof.The preparation method comprises the steps that biochar is subjected to acid treatment, and activated biochar is obtained; uniformly mixing calcium salt, ferric salt, aluminum salt and deionized water to obtain a mixed salt solution, adding urea to obtain a precursor solution, dipping the activated charcoal in the precursor solution, and then performing hydrothermal reaction to obtain loaded charcoal; mixing and grinding the oyster shell powder and dipotassium phosphate, and calcining at high temperature to obtain activated oyster shell powder; mixing the bacillus subtilis liquid, the activated oyster shell powder and trehalose, oscillating, centrifugally separating, and freeze-drying to obtain the biological composite material. Uniformly mixing the loaded biochar, the biological composite material and a carboxymethyl cellulose solution, granulating, and drying wet granules to obtain the biochemical compound type soil heavy metal pollution fixing and repairing material. According to the prepared fixing and repairing material, the fixing efficiency and adaptability to various heavy metals in soil are improved.

The invention relates to an efficient sewage purification material based on a complex microbial inoculant and a preparation method of the efficient sewage purification material, and belongs to the technical field of sewage treatment. Alkali bacillus with outstanding stress tolerance constructs a stable and adaptive micro-ecological environment for heterotrophic nitrification compound bacteria and denitrification compound bacteria, ammonia nitrogen can be efficiently converted into nitrate nitrogen and then reduced into nitrogen, the nitrogen removal effect is greatly improved, meanwhile, functional bacteria degrade organic pollutants through metabolism, and the nitrogen removal effect is improved. An adsorption and degradation synergistic mechanism is formed by the microbial inoculum, a porous structure of the modified biochar and rich oxygen-containing functional groups, the biochar firstly efficiently adsorbs heavy metals and refractory organics in sewage, the concentration of pollutants is concentrated to construct an efficient reaction microenvironment, degradation of the organics by the microbial inoculum can effectively avoid adsorption saturation of the biochar, and the microbial inoculum and the biochar supplement each other, so that the biochar can be degraded more efficiently. And finally, synchronous purification of nitrogen and organic matters is achieved, and the method is suitable for removal of ammonia nitrogen in high-salt sewage.

The invention discloses saline-alkali-resistant water-soluble pesticide fertilizer powder and a preparation method thereof. The powder is prepared from a modified humic acid polyaspartic acid composite carrier, a water-soluble pesticide active ingredient included by hydroxypropyl-beta-cyclodextrin, chelated medium trace elements, saline-alkali-resistant functional bacterial powder, a pH buffer aid and water-soluble inorganic nutrients. The composite carrier is prepared by cross-linking the composite carrier with calcium polyaspartate after being subjected to alkaline hydrolysis and oxidation of weathered coal, the functional bacterial powder is prepared by compounding salt-tolerant bacillus and pseudomonas in a specific ratio, and the pH buffer aid and the medium trace elements are all precise compounding systems. The preparation method comprises the steps of modified humic acid solution preparation, composite carrier synthesis, material mixing, crushing and sieving. All the components have a synergistic effect, and the saline-alkali soil conditioner has the functions of saline-alkali resistance, nutrient supply and pest control, and is good in water solubility, high in nutrient utilization rate, stable in pesticide effect, simple and controllable in preparation process, suitable for saline-alkali soil crop planting and high in practicability.

This invention belongs to the field of cosmetic technology, and particularly relates to a fermented milk containing Artemisia annua with oil-controlling, acne-reducing, and anti-inflammatory effects, its preparation method, and application. The preparation method includes the following steps: S1, inoculating Tremella fuciformis seed liquid into Artemisia annua culture medium for aerobic fermentation to obtain Tremella fuciformis fermentation broth; S2, separating the Tremella fuciformis fermentation broth into bacterial cells to obtain Tremella fuciformis fermentation product filtrate; S3, inoculating Bifidobacterium longum seed liquid into the Tremella fuciformis fermentation product filtrate for anaerobic fermentation to obtain Bifidobacterium longum fermentation broth; S4, separating the Bifidobacterium longum fermentation broth into bacterial cells to obtain Bifidobacterium longum fermentation product filtrate and its bacterial cells; S5, disrupting the bacterial cells to obtain their lysate, then adding the lysate to the Bifidobacterium longum fermentation product filtrate, mixing, and obtaining Artemisia annua fermented milk. The fermented milk has significant multiple effects, including soothing sensitivity, acne reduction, oil control, anti-inflammation, antibacterial properties, and moisturizing.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.