43 results about "Photosynthetic bacteria" patented technology

The invention relates to the technical field of agricultural microorganisms, and discloses a complex microbial inoculant for improving soil micro-ecology and promoting plant growth and application thereof.The complex microbial inoculant is prepared from 30-40 parts of bacillus subtilis, 20-40 parts of pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of trichoderma and 5-10 parts of penicillium; wherein the bacillus subtilis is bacillus subtilis L11 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.31274, the preservation date is July 11, 2024, and the bacillus subtilis is classified and named as bacillus subtilis. The complex microbial inoculant for improving the soil micro-ecology and promoting the plant growth is adopted, a multi-bacterium synergistic community is constructed, the complementary metabolism advantage of different strains at the rhizosphere is utilized, the nutrient conversion and stress resistance characteristics are improved, and therefore the soil micro-ecology is improved, and the plant root development is promoted.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

The application belongs to the technical field of microbial sewage treatment, and particularly relates to an iron-carbon quantum dot / electrically active non-oxygen-producing photosynthetic bacteria / carrier hybrid cell factory and application thereof in sewage resourceization or carbon emission reduction; the iron-carbon quantum dot in the hybrid cell factory provided by the application promotes the non-oxygen-producing purple photosynthetic bacteria to utilize more light energy, and generates photo-generated electrons as an extracellular efficient electron donor source, and the photosensitive active substances in the extracellular polymeric substance of the non-oxygen-producing purple photosynthetic bacteria can promote the formation of a continuous cycle of trivalent iron and divalent iron, realize mutual cooperation, promote sewage resourceization and carbon emission reduction, and synthesize high-value-added products; and in addition to facilitating the recovery of biomass, the transparent conductive glass electrode as the carrier also ensures that the non-oxygen-producing purple photosynthetic bacteria stably and efficiently utilize light energy; therefore, the hybrid cell factory provided by the application is a high-efficiency microbial sewage treatment technology, and solves the technical problem of lack of high-efficiency microbial sewage treatment technology in the prior art.

The invention discloses a bacteria-algae dual-purpose culture device, and belongs to the technical field of bacteria and algae culture, the bacteria-algae dual-purpose culture device comprises a reactor and a material loading tank, the reactor is provided with a breathing tube, a jacket, a feeding tube, a first stirring mechanism and a material receiving tube, the breathing tube is provided with a breathing pneumatic valve, the feeding tube is connected with the material loading tank through a material loading tube, the material loading tank is provided with a second stirring mechanism, and the material receiving tube is connected with the second stirring mechanism. An outer ring cleaning mechanism, an inner ring cleaning mechanism, multiple sets of lighting assemblies and a smooth system arranged outside the lighting assemblies are arranged in the reactor, the jacket and the multiple sets of lighting assemblies are connected with a cooling system, and the outer ring cleaning mechanism and the inner ring cleaning mechanism are connected with a feeding pipe through an outer ring cleaning pipe and an inner ring cleaning pipe correspondingly. The smooth system is connected with a compressed air pipe, and the feeding pipe is further connected with a tap water pipe and a pure water pipe. The device can be used for culturing photosynthetic bacteria and algae at the same time; the device has the advantages of high culture efficiency, high utilization rate of algae culture land and the like.

The application discloses a process for removing pollutants in factory breeding tail water of penaeus vannamei, and belongs to the technical field of breeding tail water treatment. The process comprises the following steps: (1) filtering breeding wastewater to remove solid matters; (2) adding composite bacteria to the filtered breeding wastewater for aeration treatment to obtain aeration wastewater, wherein the composite bacteria comprise, by weight, 1-2 parts of rhodococcus, 2-3 parts of bacillus subtilis, 3-4 parts of halococcus and 0.8-1.5 parts of photosynthetic bacteria; (3) adding a flocculating agent to the aeration wastewater, treating the aeration wastewater at 45-50 DEG C for 0.5-1 h, and filtering to obtain a first filtrate; (4) adding modified activated carbon to the first filtrate, treating the first filtrate at 45-50 DEG C for 1-2 h, filtering to obtain a second filtrate; and (5) flowing and mixing the second filtrate through a macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. The application realizes efficient removal of pollutants in the breeding tail water of penaeus vannamei through the combination of physical, chemical and biological treatments.

The present application belongs to the field of environmental remediation technology, and particularly relates to a method for ecological remediation of heavily polluted water body with bottom mud. The method for ecological remediation of heavily polluted water body with bottom mud comprises the following steps: S1: putting heavy metal remediation agent into the water body; S2: putting slow-release type bottom mud remediation agent into the water body; S3: sequentially putting algae-controlling microorganism, photosynthetic bacteria and EM bacteria, and plankton into the water body; S4: planting aquatic plants according to water depth; planting emergent plants in the area with water depth less than 0.5 m, and planting submerged plants in the area with water depth greater than or equal to 1.5 m; and S5: when the coverage rate of aquatic plants in the water body is greater than or equal to 80%, putting aquatic animals into the water body. In the present application, slow-release type bottom mud remediation agent and heavy metal remediation agent are used to reduce the pollution of bottom mud, organic matter, etc., the transparency of the water body is then improved after reduction, aquatic plants are then planted, and finally aquatic animals are put into the water body, so as to construct a water ecological system and achieve the purpose of ecological remediation of the water body.

The invention relates to the technical field of fertilizers, and discloses a biological protein principle-based fertilizer for preventing and treating citrus liberobacter asiaticum, which is prepared from the following raw materials in parts by weight: 40 to 70 parts of fish protein peptide fermentation products, 5 to 15 parts of EM flora active liquid, 1 to 5 parts of chelated trace element solution and 10 to 20 parts of algal polysaccharide-polyglutamic acid compound. The fish protein peptide is fermented for 180 days or longer through EM bacteria. The EM flora active liquid contains lactic acid bacteria, photosynthetic bacteria, actinomycetes and saccharomycetes. The trace elements are at least two of boron, zinc, iron, manganese and molybdenum and are chelated by polyglutamic acid or sugar alcohol. The small peptide, the bacterial metabolite and the chelate element are in slow release synergy, so that rhizosphere microecology can be rebuilt, defensive enzymes can be induced, and the phenomena of huanglong, yellowing and red nose can be relieved.

The invention discloses preparation and application of a plant element insecticide extracted based on a microbial extraction technology, and relates to the technical field of insecticide preparation, the preparation steps are as follows: S1, preparing raw materials including 10-900 parts of a basic microbial agent, 10-700 parts of nutrient substances, 10-500 parts of plant raw materials, 10-400 parts of a functional microbial agent and 10-300 parts of an enzyme preparation; the basic fungicide and the nutrient substances respectively comprise activated EM symbiotic bacteria, molasses, yeast, flour photosynthetic bacteria, diammonium phosphate, mineral source potassium fulvate, lactic acid bacteria and amino acid stock solution. According to the method, after the soil fertility is improved through microorganisms, the soil is activated, and the microbial insecticide containing plant elements is applied, the microorganisms are directly added to the soil, various animal and plant residues and mineral substances in the soil can be more effectively decomposed through supplementation of the soil microorganisms, soil humus is rapidly formed, and the structure and physical and chemical properties of the soil are improved; pest control is obvious; the microbial insecticide containing the plant elements contains a large number of beneficial microorganisms.

The invention discloses a photovoltaic pond river crab culture method, which comprises the following steps: a bacteria-algae compound conditioning agent is put into a target photovoltaic pond according to a preset period and a preset putting dosage, the bacteria-algae compound conditioning agent comprises an effective microbial flora microbial agent and a chlorella complex which are mixed according to a preset proportion, the pH value of the effective microbial flora microbial agent is 3.0-4.0, the pH value of the chlorella complex is 3.0-4.0, and the pH value of the chlorella complex is 3.0-4.0. The content of lactic acid bacteria in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of saccharomycetes in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of photosynthetic bacteria in the effective microbial agent is 1.0 * 10 to 2.0 * 10 < 3 > CFU / mL, the content of actinomycetes in the effective microbial agent is 1.0 * 10 < 3 > to 3.0 * 10 < 3 > CFU / mL, and the concentration of chlorella in the chlorella complex is 1.0 * 10 cells / mL; and monitoring and dynamically adjusting the water quality in the target photovoltaic pond, and adjusting the putting frequency and the putting dosage of the bacteria-algae compound regulator within a preset range according to a monitoring result so as to maintain or restore the ecological balance of the water body and the river crabs. According to the technical scheme, the problem of micro-ecological unbalance of photovoltaic shading is solved, oxygen is supplied by algae, diseases are inhibited by bacteria, and carbon sequestration by bacteria and algae is realized, so that the breeding survival rate is increased, and the growth of river crabs is promoted.

The invention provides a composted straw composite polysaccharide microbial agent as well as a preparation method and application thereof, and belongs to the technical field of microbial agents. The composted straw composite polysaccharide microbial inoculum is prepared from the following raw materials in parts by weight: 3-5 parts of soybean oligosaccharides, 1-1.5 parts of yeast zinc, 2-3 parts of photosynthetic bacteria flora, 2-3 parts of lactic acid bacteria flora, 5-6 parts of EM bacteria, 70-72 parts of animal amino acids and 10-15 parts of trehalose. The polysaccharide is added to assist microbial fermentation, so that the microbial fermentation speed is remarkably increased, the time required for straw decomposition is shortened, the soil fertility is improved, the crop quality is improved, and diseases are reduced.

The invention discloses a microorganism for treating moss and foul smell in park landscape lake water and a culture process thereof, the microorganism comprises biphase photosynthetic bacteria, decomposing bacteria and a culture medium, the decomposing bacteria comprise bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium, the weight percentage of the decomposing bacteria is 1-5%, and the weight percentage of the decomposing bacteria is 1-5%. The microbial inoculant comprises the following components in percentage by weight: 20-30% of biphasic photosynthetic bacteria, 15-25% of bifidobacterium, 10-20% of mesophilic actinomycetes, 10-20% of nitrosobacteria and 15-25% of bacillus megatherium. The biphase photosynthetic bacteria and the composite decomposition bacteria are scientifically proportioned to form a microbial system with complementary functions, the biphase photosynthetic bacteria can directionally inhibit photosynthesis of moss, destroy cell structures of moss and realize targeted killing, and the decomposition bacteria synergistically decompose organic matters such as underwater residual feed, excrement, animal and plant bodies and the like, so that the biological activity of moss is improved. Meanwhile, nitrosobacteria can convert algal toxins into harmless substances, the synergistic effect overcomes the defects that an existing single microbial preparation is single in function and incomplete in treatment, and integrated treatment of killing moss, eliminating stink and improving water quality is achieved.

The present application relates to the field of agricultural microorganism technology, and discloses a composite microbial agent for improving soil micro-ecology and promoting plant growth and application thereof.The composite microbial agent comprises 30-40 parts of Bacillus subtilis, 20-40 parts of Pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of Trichoderma and 5-10 parts of Penicillium; wherein the Bacillus subtilis is Bacillus subtilis L11, which is preserved in the China General Microbiological Culture Collection Center on July 11, 2024, with the preservation number of CGMCC No.31274 and the classification name of Bacillus subtilis The present application adopts the composite microbial agent for improving soil micro-ecology and promoting plant growth, constructs a multi-bacterial synergistic community, utilizes the complementary metabolic advantages of different strains in the rhizosphere, improves nutrient transformation and stress resistance characteristics, and thus improves soil micro-ecology and promotes plant root development.

The invention discloses a process for removing pollutants in industrial aquaculture tail water of penaeus vannamei boone, and belongs to the technical field of aquaculture tail water treatment. Comprising the following steps: (1) filtering breeding wastewater to remove solids; (2) compound bacteria are added into the filtered breeding wastewater for aeration treatment, aerated wastewater is obtained, and the compound bacteria comprise rhodococcus ruber, bacillus subtilis, brevibacterium halotolerans and photosynthetic bacteria according to the weight ratio of (1-2): (2-3): (3-4): (0.8-1.5); (3) adding a flocculating agent into the aerated wastewater, treating at 45-50 DEG C for 0.5-1 hour, and filtering to obtain a first filtrate; (3) adding modified activated carbon into the first filtrate, treating at 45-50 DEG C for 1-2 hours, and filtering to obtain a second filtrate; and (4) treating the second filtrate through mixed macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. By combining physical, chemical and biological treatment, pollutants in the penaeus vannamei culture tail water are efficiently removed.

The invention relates to a high-quality rice and crab planting and breeding method which comprises the following steps: transforming a rice field, building a rice and crab symbiotic living environment, and arranging water feeding and draining and escape-preventing facilities; cleaning the rice field, and keeping the water level stable and the water quality standard; aquatic plant cultivation is beneficial to inhabiting, hiding, growing and shelling of the crab species, so that the survival rate of the crab species is increased; planting rice, selecting a variety suitable for local planting, weeding before planting, using a pre-seedling transplanting method, properly increasing the transplanting density around ridges and two sides of surrounding ditches, sufficiently applying base fertilizer, and reducing topdressing; juvenile crabs are stocked, juvenile crabs are temporarily bred in circular ditches, and the juvenile crabs are mainly prepared from compound feed and preferably prepared from fresh and alive bait; performing ecological regulation and control on the rice and crab fields: regularly putting photosynthetic bacteria into the rice and crab fields; disease control: biocontrol bacteria and immune polysaccharides are added into the feed, so that the resistance of the river crabs is improved; daily management and scientific crab catching and rice harvesting are conducted, river crabs start to be caught in late September, rice is harvested after catching is completed, and by optimizing the planting and breeding process, the method has the advantages of being high in yield, large in size and low in disease rate.

The invention belongs to the technical field of ecological restoration, and particularly relates to a mine regreening method which is used for carrying out regreening treatment on a to-be-regreening area of a mine, and the mine regreening method comprises the following steps: preparing a microbial bacterium solution which contains sulfate reducing bacteria, photosynthetic bacteria and nitrogen-fixing bacteria; carrying out amplification culture on the microbial liquid until the concentration is higher than the required concentration; the to-be-greened area comprises a flat area and a slope area, and spraying pipes are arranged in the flat area and the slope area respectively; spraying the microbial bacterial liquid subjected to amplification culture to the flat area and the slope area in stages by using a spraying pipe; a first plant capable of extending and growing from top to bottom in the inclination direction of the slope area is planted at the top of the slope area, a second plant capable of extending and growing from bottom to top in the inclination direction of the slope area is planted at the mountain foot of the mine, and a third plant used for blocking gravel falling from the slope area is planted in the flat area. By the adoption of the mine greening method, greening of the mine preparation damaged area can be effectively completed.

The application discloses a feed for feeding bullfrog, which comprises photosynthetic bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protein hydrolytic enzyme; the sugar component comprises fructose and glucose; the bone source component is bovine bone powder; and the auxiliary nutritional component is at least one selected from vitamin premix, mineral premix and amino acid premix. The formula is scientific and reasonable, and the feed is comprehensive and balanced in nutrition. The biological bacteria, sugar, bone source and various auxiliary nutritional components synergistically act to meet the requirements of bullfrog at various growth stages. The added biological bacteria and enzymes can improve the digestion and utilization rate, reduce the nutrition waste and breeding cost. The antioxidant and the mildew-proof agent guarantee the good storage performance of the feed, prolong the shelf life and ensure the edible safety. The raw materials and production are strictly controlled, the biological safety is enhanced, and the disease risk is reduced.

The invention discloses an airlift fermentation tank with a tail gas CO2 recovery and recharge structure, and relates to the technical field of airlift fermentation tanks, the airlift fermentation tank comprises a tank body, a dehydration mechanism, a condenser, a CO2 trapper and a defoaming mechanism, the tank body is connected with the dehydration mechanism, the condenser, the CO2 trapper and the defoaming mechanism through pipelines, and the dehydration mechanism, the condenser, the CO2 trapper and the defoaming mechanism are connected through pipelines. A guide cylinder is arranged in the tank body, the dewatering mechanism comprises an outer cylinder, an annular groove is formed in the upper portion of the inner wall of the outer cylinder, an annular body is rotatably installed in the annular groove in an embedded mode, the lower inner edge of the annular body is connected with a centrifugal cylinder through a guide cover, and the lower end of the centrifugal cylinder rotatably penetrates through the bottom of the inner side of the outer cylinder. According to the airlift fermentation tank with the tail gas CO2 recycling and recharging structure, CO2 in tail gas is reintroduced into the tank body to be in contact with thalli through a CO2 recycling system, so that the problem of loss of CO2 in tail gas of a traditional airlift fermentation tank is effectively solved, meanwhile, a sufficient carbon source is provided for photosynthetic bacteria, and the fermentation efficiency and the resource utilization rate are remarkably improved.

The invention discloses a microorganism for treating cyanobacteria and stink in park landscape lake water and a culture process thereof, the microorganism comprises a microbial agent, a compound enzyme, tartaric acid and free fatty acid, and the microbial agent is composed of biphasic photosynthetic bacteria, bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium. The microbial agent is compounded with the compound enzyme, the tartaric acid and the free fatty acid, five strains in the microbial agent are clear in division of labor, the biphasic photosynthetic bacteria decompose organic matters released by cyanobacteria residues and reduce ammonia nitrogen, the bifidobacterium adjusts the balance of water flora and inhibits reproduction of malodorous bacteria, and the mesophilic actinomycetes secretes antibacterial substances to prevent recurrence of the cyanobacteria. Nitrosobacteria convert an odor source into harmless substances, bacillus megaterium further secretes extracellular enzyme to accelerate degradation of residues, and microorganisms and bioactive substances have a synergistic effect, so that the removal period of blue-green algae is shortened, and the odor removal rate is increased and is far superior to that of a single microbial preparation.

The invention discloses a high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers and lakes and a culture method, the high-standard microbial flora comprises the following biological bacteria: photosynthetic bacteria, pseudogreen photosynthetic bacteria, bacillus amyloliquefaciens and proteolytic enzyme, and the photosynthetic bacteria and the pseudogreen photosynthetic bacteria have a synergistic effect to enhance the capability of decomposing organic matters in a water body; the bacillus amyloliquefaciens is responsible for decomposing starch substances in the water body, and the proteolytic enzyme is used for hydrolyzing proteins in the water body. According to the invention, multiple strains are reasonably combined and the proportion of the strains is optimized, so that pollutants are efficiently and synergistically removed; components of the culture medium are accurately matched, and proper nutrition is provided for microorganisms; culture conditions are accurately controlled in stages, and growth requirements of different microorganisms are met; culture parameters are monitored in real time and dynamically adjusted to ensure stable and efficient treatment of microorganisms; the collected concentrated microorganisms are convenient to store and transport and keep activity; the practical treatment effect is enhanced through adaptive domestication before application.

The invention discloses a submerged slow-release type aquaculture water quality improvement composite bacterial tablet and a preparation method thereof, and relates to the technical field of aquaculture. The microencapsulated tablet comprises a microencapsulated tablet body, the microencapsulated tablet body is composed of a core material and a microcapsule sustained-release layer wrapping the core material, and the gram weight of a single agent of the microencapsulated tablet body is larger than or equal to 2 g; the core material comprises a complex microbial inoculant and a nutrient medium. Through the high specific gravity design that the gram weight of a single agent is greater than or equal to 2g, and the bentonite component in the microcapsule slow-release layer, the preparation can sink to the bottom after being thrown and directly reach a water bottom pollution core area, and the limitation that a liquid or powder preparation cannot form an effective concentration at a seriously polluted part is broken through; the bacillus subtilis, the bacillus licheniformis, the photosynthetic bacteria and the lactic acid bacteria are compounded and proportioned according to the ratio of 2: 2: 1: 1, the current situation that a traditional bacterial tablet is single in strain is changed, the strains do not antagonize each other and can achieve a synergistic effect, and the adaptability to the complex water quality problem is greatly improved.

The invention discloses a faeces water quality decomposing agent and a culture process thereof. The faeces water quality decomposing agent is prepared from the following components in parts by weight: 15 to 30 parts of photosynthetic bacteria, 10 to 20 parts of pseudogreen photosynthetic bacteria, 3.75 to 7.5 parts of bacillus amyloliquefaciens, 5 to 10 parts of proteolytic enzyme, 2 to 4 parts of humic acid, 0.5 to 1 part of sodium alginate and 1 to 2 parts of monopotassium phosphate. By optimizing the strain combination and auxiliary component proportion, an efficient and synergistic microbial ecological system is formed, various organic matters in the excrement can be quickly decomposed, and the organic fertilizer is suitable for wide organic waste treatment of human, livestock and poultry excrement and the like; a pure biological technology is adopted, the method is environment-friendly and pollution-free, and decomposed products can be used as high-quality organic fertilizers to realize resource circulation; the culture process is scientific and reasonable, parameters of each step are clear, links from strain activation to enzyme preparation and the like are all optimized, and the quality and stability of the decomposer are improved; the raw materials are wide in source and low in price, the culture process is simple, and the treatment cost is greatly reduced.

The invention provides a preparation method of a biochar-based slow-release organic fertilizer based on ceriporia lacerata degradation synergy, which comprises the following steps: S1, preparing biochar: treating forestry wastes to obtain waste powder, and pyrolyzing the waste powder to obtain the biochar; s2, bacterial strain graded loading: sequentially adding the biochar into a ceriporia lacerata bacterial solution, a photosynthetic bacteria bacterial solution and a phosphate solubilizing bacteria bacterial solution to obtain biochar BC-L-P-S; s3, composting fermentation: performing composting fermentation on biochar BC-L-P-S, humic acid and the forestry waste powder prepared in the S1, and drying and crushing after fermentation to obtain fertilizer powder; s4, granulation and pH response layer loading: mixing the fertilizer powder, bentonite, diatomite and xanthan gum to prepare organic fertilizer particles; putting the organic fertilizer particles into a fluidized bed coating machine, spraying the pH response layer solution, and drying to obtain the organic fertilizer. According to the organic fertilizer, the utilization rate of the organic fertilizer is increased, and great potential is shown in the aspects of promoting crop growth, improving soil quality, reducing environmental pollution and the like.

The invention discloses a deodorant and a preparation method thereof. The deodorant comprises the following components in parts by weight: 10%-20% of photosynthetic bacteria, 5%-15% of streptococcus lactis, 5%-15% of bacillus amyloliquefaciens, 10%-20% of proteolytic enzyme, 15%-25% of compound enzyme, 10%-20% of tartaric acid and 5%-15% of free fatty acid. By adopting the synergistic effect of various microorganisms, enzymes and chemical components, the deodorant is efficient in deodorization and can quickly remove various stink; environment-friendly and safe components are selected, pollution to the environment is avoided, harmful residues are avoided, and the water purifying agent can be safely used for various water bodies and does not harm aquatic organisms and human health; the water quality improvement function is achieved, organic pollutants can be decomposed, nutrients are balanced, and the water quality is improved; the formula is high in adaptability, has a good effect on different types of odor sources, and is wide in application range; the preparation process is scientific and reasonable, operation steps and parameters of all links are clear, stable and reliable quality is guaranteed, and large-scale production and popularization are facilitated.

The invention discloses an application of a film-embedded conjugated oligomeric electrolyte in promoting electron transfer of non-photosynthetic bacteria and enhancing carbon sequestration capability. The structural formula of the film-embedded conjugated oligomeric electrolyte is shown as a formula I in the specification. According to the invention, the conjugated oligomeric electrolyte which has photophysical activity and can be embedded into bacterial cell walls is combined with the non-photosynthetic microorganisms, and photoinduced electron or excited state energy transfer generated by the material under illumination is utilized to promote the electron transfer process in non-photosynthetic microorganism cells, so that the reducing power supply of the non-photosynthetic microorganisms is enhanced; the CO2 fixation metabolic pathway is activated or enhanced, and finally efficient and green bioconversion of CO2 into valuable chemicals is realized.

The invention discloses a method for producing PHA (polyhydroxyalkanoate) by fermenting photosynthetic bacteria cultured by using a mixed carbon source. The method comprises the following steps: inoculating phototrophic bacteria into a photobioreactor filled with an inorganic salt culture medium taking CO2 as a unique carbon source, and introducing CO2 under illumination to carry out photoautotrophic culture, so that the phototrophic bacteria proliferate to a stable growth period; inducing the phototrophic bacteria to secrete extracellular sugar through salt stress until the concentration of the extracellular sugar reaches a preset threshold value; inoculating heterotrophic bacteria into the culture medium, co-culturing the heterotrophic bacteria and phototrophic bacteria, culturing by taking CO2 as a unique carbon source, supplementing extracellular sugar, and continuously culturing until PHA accumulation reaches a peak value; finally, thalli are collected, and intracellular PHA is extracted. According to the method, phototrophic bacterium growth and sugar production and heterotrophic bacterium PHA accumulation are separated and coupled in time and function, extracellular sugar supplement is started in the later stage of co-culture, CO2 and an organic carbon source are synergistically utilized, the method can stably operate in 5L and 20L photobioreactors, the advantage of carbon emission reduction in the early stage is kept, and the yield and the production intensity are improved through organic carbon supplement.

This invention relates to the field of crop yield enhancement technology, specifically to a drought-resistant fertilizer-bacterial compound foliar fertilizer for wheat and its preparation method. It is prepared from the following raw materials in parts by weight: 54-60 parts of basic foliar fertilizer solution, 5 parts of plant-derived carbon-nitrogen solution, 5 parts of potassium humate solution, 20 parts of photosynthetic bacteria agent, 0.05 parts of polyether-modified trisiloxane, 1 part of polyethylene glycol 2000, 0.3 parts of xanthan gum, and 8.65-14.65 parts of deionized water containing 0.001% 28-homobrassinolide. The basic foliar fertilizer solution includes 12-15 parts of urea, 9-12 parts of potassium dihydrogen phosphate, 0.4 parts of trace elements, and 32.6 parts of deionized water. This invention solves the problems of existing wheat foliar fertilizers having limited function, failing to simultaneously achieve multiple effects such as nutrient supplementation, improved drought resistance, increased photosynthetic efficiency, and stress-resistant yield enhancement, as well as the problems of insufficient spreadability and low absorption and utilization rates due to unscientific adjuvants.

The application discloses a method for producing PHA by fermenting photosynthetic bacteria with CO2. The method comprises inoculating phototrophic bacteria into a photobioreactor containing an inorganic salt medium with CO2 as the sole carbon source, and performing photosynthetic autotrophic culture under illumination and CO2 aeration to proliferate the phototrophic bacteria to the stable growth phase. Then, the phototrophic bacteria are induced to secrete extracellular sugar through salt stress, and the culture is performed until the extracellular sugar concentration reaches a predetermined threshold. Then, heterotrophic bacteria are inoculated into the culture to form a co-culture system with the phototrophic bacteria, and the culture is continuously performed under illumination and CO2 aeration, so that the heterotrophic bacteria synthesize and accumulate PHA by using the extracellular sugar. Finally, the bacterial cells are collected, and intracellular PHA is extracted. The method separates and couples the growth and sugar production of the phototrophic bacteria and the PHA accumulation of the heterotrophic bacteria in time and function, realizes the biosynthesis of PHA with CO2 as the sole carbon source, solves the metabolic contradiction that a single microorganism cannot simultaneously and efficiently fix carbon and synthesize PHA, and can stably operate in a 5L and 20L photobioreactor.

The invention discloses a water decomposing agent and a culture method. The decomposing agent is prepared from the following components in percentage by mass: 30-50% of a functional microorganism composition, 45-65% of a composite carrier material and 3-8% of an auxiliary additive, wherein the functional microorganism combination comprises the following components in percentage by mass: 35%-45% of nitrifying bacteria, 25%-35% of nitrosobacteria, 15%-25% of biphase photosynthetic bacteria and 15%-25% of rhodococcus faecalis; the composite carrier material comprises the following components in percentage by mass: 50%-60% of porous ceramsite, 20%-30% of activated carbon, 10%-20% of diatomite and 1%-5% of a trace element additive. According to the compound microorganism water quality decomposing agent, through multi-strain cooperation, carrier optimization and process innovation, the comprehensive advantages of efficient degradation of pollutants, wide environmental adaptability, long-acting stability, low-cost operation and the like are achieved, and the compound microorganism water quality decomposing agent can be widely applied to the fields of sewage treatment, aquaculture, landscape water body remediation and the like and has remarkable market competitiveness and social benefits.

The application discloses a kind of compound microbial inoculant culture device and method, and culture device includes culture tank and adjusting mechanism.The top of culture tank is equipped with feeding port and air vent, and the bottom is equipped with discharge port;Adjusting mechanism includes adjusting plate, clamping plate, rectangular frame and moving block, clamping plate is equipped with adjusting plate slot, rectangular frame slot and moving block slot in sequence, adjusting plate is located in adjusting plate slot, rectangular frame is located in rectangular frame slot, and moving block is located in moving block slot;The bottom of adjusting plate is inclined plane, and the outer wall of roller is tightly attached to inclined plane and the top of moving block simultaneously, the left wall of rectangular frame is connected to clamping plate by first spring, the right wall of rectangular frame is equipped with air vent conversion plate, the top of moving block is equipped with horizontal area and inclined area, the bottom of moving block is equipped with push rod, and push rod is connected to sealing plate after penetrating the bottom of clamping plate, and the other end of sealing plate is located at discharge port.It can be simultaneously mixed and symbiotic culture for lactic acid bacteria, photosynthetic bacteria, yeast and nitrogen-fixing bacteria, etc., and a large number of proliferation and keep strain activity.