381 results about "Bacillus aryabhattai" patented technology

The invention discloses a hametz used for fermenting tobacco and applications thereof. The hametz is characterized by comprising three strains of Bacillus subtilis, lactobacillus and microzyme, the viable count ratio of which is 10 to 50: 2 to 30: 2 to 30. The hametz can ferment waste material of the tobacco or the flavor used for producing natural cigarettes by using the tobacco and can be used for tobacco fermentation. The flavor used for the natural cigarettes prepared by the hametz has a velvet fragrance and can remarkably increase the fragrance, reduce the unpurified gas and reduce the thrill as well when the flavor is used in the cigarettes, thus leading the smoke gas to be lingering and smooth. The hametz used for fermenting tobacco leaves can remarkably shorten the alcoholizing time and improve the quality of the tobacco leaves.

Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

The invention discloses a complex probiotics for environmental remediation, a preparation method and application thereof. The complex probiotics is prepared from the following strains in weight proportion: 1 to 5 portions of photosynthetic bacteria, 1 to 3 portions of lactic acid bacteria, 0.5 to 1.5 portions of bacillus, 1.0 to 1.4 portions of actinomycete, 0.6 to 1.0 portion of microzyme, and 1 to 5 portions of filamentous fungus, wherein the contents of active bacteria in the strains are all between1,800 and 2,600 million per milliliter. The mechanism of action of the complex probiotics comprises that: the probiotics is used as a leading raw material and combined with other useful microbes to generate an oxidation resisting material, and the oxidation resisting material decomposes and oxidizes organic matters by oxidization, reduction, fermentation and the like, so that the harmful and poisonous materials are turned into unharmful and nontoxic materials, and the idea of changing wastes into valuables is realized. The complex probiotics is widely applied in environmental protection field of deodorizing in a washroom, reutilizing domestic garbage, treating odorous lakes and stagnant water, purifying tap water and a swimming pool and the like, and has low cost.

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

The invention belongs to the field of microbial resources, and particularly discloses a strain of Bacillus aryabhattai J5 and application thereof in promoting growth of corn. The Bacillus aryabhattai J5 is collected in CGMCC (China General Microbiological Culture Collection Center) on October 15th, 2015 and is filed with the number of CGMCC NO.11508. The Bacillus aryabhattai J5 has functions of producing auxin, siderophore, ammonia and protease, dissolving phosphorus, resisting to salt, realizing antagonism, and the like. As the strain is capable of growing normally in a culture medium with salt content equal to 11%, the strain has good potential in eliminating soil salinization. As content of siderophore produced by the strain is up to 53.7%, the strain has effective disease-prevention property. By the strain, corn seed germination rate can be increased by 12.5%, and accordingly the strain has good growth promoting effect. By application of microbial inoculum prepared from the strain, corn yield is increased by 37.1%, and hundred-grain weight is increased by 35%. It is possible to develop the strain into microbial inoculum, and accordingly the strain has wide application prospect in terms of improving soil nutrient, eliminating salinization, promoting growth of plants, preventing diseases and the like.

The invention relates to a bio-organic fertilizer and a method for preparing the bio-organic fertilizer which prevents and treatments soil insects. The bio-organic fertilizer is added with active bacteria and other nutrition bacterial genus, wherein the active bacteria and other nutrition bacterial genuses can prevent and treat soil insects. The bio-organic fertilizer comprises biological agents and organic fertilizers, wherein the microbial agents comprise bacterial colonies of Japanese beetle bacilli, withered grass bacilli, enormous bacilli, jelly-formed bacilli, brown ball nitrogen-fixing bacteria and bacillus licheniformis. The Japanese beetle bacilli are the main type of bacterium which prevents and treats soil insects and other types of bacteria are mainly used for fixing nitrogen, decomposing phosphorus, decomposing potassium, and preventing and treating soil-borne diseases. The organic fertilizer comprises livestock and poultry manure and edible mushroom dregs. The livestock and poultry manure and the edible mushroom dregs are mixed, ferment and are thoroughly decomposed, the microbial agents are added and the bio-organic fertilizer is prepared. The bio-organic fertilizer and the method for preparing the bio-organic fertilizer which prevents and treatments soil insects can effectively kill larvas of soil insects. Bacterial colonies of microorganisms grow by fixing nitrogen, decomposing phosphorus and decomposing potassium, the structure of soil is improved, the organic content of soil is improved, the quality of crops is improved and the crop yield is improved.

The invention relates to a compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate and a preparation method thereof. The use amount of calcium nitrate is reduced, and the time for treating the black and odorous rivers is shortened. The compound microbial preparation is prepared from 40-80 parts of thiobacillus denitrificans and thiocapsa roseoppersicina mixed powder, 10-60 parts of composite bacillus subtilis powder and 10-60 parts of yeast and lactic acid bacterium mixed powder. By means of the preparation, sulfide in black and odorous bottom sludge, organic matter and strains suitable for growing in the black and odorous bottom sludge are screened and degraded in a targeted mode; by means of thiobacillus denitrificans with efficient hydrogen sulfide degrading and denitrification capacity, sulfur-oxidizing bacteria of thiocapsa roseoppersicina with efficient hydrogen sulfide and ammoniacal nitrogen degrading capacity, bacilli rich in protease, amylase, cellulose and other enzyme systems, yeast and lactic acid bacteria, a small amount of calcium nitrate and the compound microbial preparation are thrown into the black and odorous rivers so that water quality of the black and odorous rivers can be quickly improved, the black and odorous bottom sludge can be removed quickly, and the thickness and the organic matter content of the bottom sludge can be quickly reduced.

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

The present invention relates to a composite microbial agent, a preparation method thereof and an application in the preprocessing of kitchen garbage. The active ingredients of the composite microbial agent include 15 percent to 30 percent of bacillus stearothermophilus, 10 percent to 25 percent of bacillus licheniformis, 10 percent to 25 percent of zymomonas, 10 percent to 25 percent of agrobacterium, 15 percent to 30 percent of saccharomyces cerevisiae and 10 percent to 25 percent of candida, which are blended together. The composite microbial agent is prepared according to the following method: (1) the strains, which are preserved under the temperature of 4 DEG C, are respectively inoculated in ordinary broth media, the inoculation amount is 0.1 percent (v/v), and under the temperature between 28 DEG C and 37 DEG C, activating culture lasts for 8 to 18 hours; (2) the strains are then respectively transinoculated into ordinary broth media to be cultured for 8 to 18 hours under the temperature between 28 DEG C and 34 DEG C, the inoculation amount is between 0.1 percent (v/v) and 0.5 percent (v/v), and after culturing, microbial liquids are blended according to the proportion. The composite microbial agent can be used to preprocess kitchen garbage, inhibit the reproduction of pathogenic microorganisms in the kitchen garbage and reduce the deterioration and stink of the kitchen garbage, so that the kitchen garbage can be conveniently processed later, so the application prospect of the composite microbial agent is wide.

The invention discloses a microbial decomposition agent for animal wastes and straws and a preparation method thereof. The microbial decomposition agent comprises the following strains in parts by weight: bacillus, saccharomycetes, Aspergillus niger, Aspergillus oryzae, trichoderma sp, lactic acid bacteria, photosynthetic bacteria and actinomycetes. The decomposition agent has diversified functions, can be simultaneously used for composting straws and/or animal wastes, contains multiple functional bacteria and is high in microbe activity, the selected strains achieve a synergistic effect, do not generate antagonism, and the decomposition agent has the advantages of fast temperature rise and high temperature of fermented materials, complete decomposition, short decomposition time and the like. The preparation method of the microbial decomposition agent disclosed by the invention has the advantages of simplicity, feasibility, simple operation and low production cost.

The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The invention relates to a bacillus simplex YM8 CGMCC No.5242, and a culture method and application thereof. The culture method for the bacillus simplex YM8 comprises the following steps: inoculating the strain into a seed culture medium; cultivating in a shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm until the strain concentration reaches 10<8> to 10<9>/mL; inoculating 5 mL of bacterial liquid into a culture medium; and cultivating in the shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm. The bacillus simplex YM8 can be applied to environmental management on the oil field, and has a good effect of degrading crude oil, and higher research, application and market values. The culture method is simple and easy to operate, quick in growth and propagation and practicable in expanding production.

The invention provides bacillus aryabhattai and an application thereof in preventing and treating tobacco black shank. The bacillus aryabhattai provided by the invention is named as bacillus aryabhattai J-6, and has been collected in a collection unit; the name of the collection unit is China Center for Type Culture Collection, CCTCC for short; the collection date is October 17, 2013; the collection number is CCTCC NO: M2013475. The bacillus aryabhattai J-6 is applied to preventing and treating the tobacco black shank. The bacillus aryabhattai provided by the invention is safe to human, livestock and crops, and friendly to the environment, and also has an obvious effect preventing and treating the tobacco black shank.

The invention discloses a bacillus licheniformis and application of the bacillus licheniformis. The bacillus licheniformis provided by the invention is concretely bacillus licheniformis C30-2 with the preservation number being CGMCC (China General Microbiological Center Culture Collection Center) No.5350 in CGMCC. Experiments prove that the bacillus licheniformis C30-2 CGMCC No.5350 provided by the invention has strong stress resistance and strong sundry fungus resistance capability. The bacillus licheniformis C30-2 CGMCC No.5350 is emulsified for preparing the bacillus licheniformis preparation, the bacillus licheniformis preparation can be used as additives used for preparing animal feed and can replace antibiotics in animal daily ration for regulating the microecological balance in the animal intestinal tracts, so the effect of enhancing the nonspecific immunity function for realizing the effect of preventing the diseases is realized, and meanwhile, nutrition factors can also be provided for promoting the digestion absorption of nutrition substances, reducing the diarrhea, promoting the animal growth and improving the animal feed conversion rate.

The invention provides a salt-resistance Bacillusmethylotrophicus strain LJ and an application thereof. The salt-resistance Bacillusmethylotrophicus strain LJ is classified to be named as Bacillusmethylotrophicus, and the collection number of the salt-resistance Bacillusmethylotrophicus strain LJ is CGMCCNO.7570. By performing salt tolerance analysis on the salt-resistance Bacillusmethylotrophicus strain LJ, performing a pathogenicity test on crops, and performing a test on the inhibition effect of the strain on pathogenic bacteria, results show that the salt tolerance degree of the strain is 25-30%; the strain can not cause pathogenicity to the crops, has relatively ideal inhibition effects on bacterial diseases, viral diseases and fungous diseases of tobaccos, can be compounded with a great number of elements, is long in retention period and high in effective bacterium survival rate, can be applied to saline-alkali soils, and has the effects of improving the soil, resisting the diseases, increasing the yield and the like. The salt-resistance Bacillusmethylotrophicus strain LJ disclosed by the invention ensures that a microbial preparation has the positive effects of preventing the diseases and promoting the growth of plants in special environments; the microbial inoculum has the effects of salt-resistant growth-promoting bacteria and disease-prevention growth-promoting bacteria, and is suitable for improvement of salt marsh, alkali soil and acid soil.

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The invention provides a holothuria scabra artificial seeding breeding method. The method comprises the steps such as parent holothurian cultivating, induced spawning, spawn washing and hatching, larva cultivating, seedling collecting and childish holothurian cultivating, wherein spirulina powder, marine yeast, aquatic yeast and bacillus serve as bait, not only are bait sources convenient, but also the investment of single cell culture facilities is saved, meanwhile, manpower needed by single cell algae bait culture is saved, the holothurian seedling cost can be greatly saved, and the popularization of the holothuria scabra seedling breeding technology is facilitated. The substitutive bait is adopted to replace living single cell algae, invested funds of seeding facilities can be saved by about 25 percents, the human input of seeding can be saved by about 30 percents, and the ratio of the seeding input to the seeding output 1:3. Induced spawning is performed on the two days before or after the first day and the fifteenth day of a lunar month, the good effect of two times of spawn sampling in one month is achieved, the induced spawning success rate is 83.3 percents, and the technical problem that the induced spawning difficulty of holothuria scabra parent holothurian is high is effectively solved.

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

The invention provides lysinibacillus fusiformis, the collection number of which is CGMCC (China General Microbiological Culture Collection Center) NO.8835. The invention further provides a microbial agent which contains thalli and a culture medium, wherein the thalli comprises the lysinibacillus fusiformis the collection number of which is CGMCC NO.8835. The invention further provides applications of the lysinibacillus fusiformis and the microbial agent in preparing a microbial fertilizer. Through the technical scheme, the fertilizer efficiency of a phosphorous fertilizer can be more effectively increased.

The invention discloses bacillus subtilis for accumulating N-acetylneuraminic acid to recombine and an application thereof, and belongs to the field of genetic engineering. According to the bacillus subtilis, bacillus subtilis 168-delta-nagP delta-nagP delta-gamP delta-gamA delta-nagA delta-nagB delta-1dh delta-pta:: lox72 are taken as expression hosts, through the over-expression of glucosamine acetylase coding geneS GNA 1 which are derived from saccharomyces cerevisiae S288C, N-acetyl-glucosamine epimerase (AGE) which is derived from anabaena sp. CH1 and N-N-acetylneuraminic acid synthase (NeuB) which is derived from escherichia coli K1, genetically engineered bacteria of the bacillus subtilis for accumulating N-acetylneuraminic acid are obtained, the yield of N-acetylneuraminic acid reaches 190mg/L, and bacillus subtilis further lays a foundation for the process that the bacillus subtilis is transformed by metabolic engineering to produce the N-acetylneuraminic acid.

The invention discloses a household harmful microorganism elimination and deodorization solution and a preparation method thereof. The household harmful microorganism elimination and deodorization solution comprises the following components in part: 13 to 21 parts of photosynthetic bacteria, 11 to 16 parts of bacillus, 23 to 31 parts of lactic acid bacteria, 17 to 24 parts of yeast, 11 to 16 parts of nitrifying bacteria and 10 to 15 parts of actinomycetes. Meanwhile, the invention also discloses the preparation method of the household harmful microorganism elimination and deodorization solution. According to the household harmful microorganism elimination and deodorization solution disclosed by the invention, various group bacteria which are beneficial to win-win cooperation of floras and have synergism are formed by self expanding culture and mixed expanding culture; by the expanding culture acid production, the pH is reduced to 3.6 to 4.8; and various harmful microorganism elimination and deodorization extension derivatives are also generated. Therefore, various harmful microorganisms in the environments of living, working and office places of people, restaurants for business activities, hotels, stations, public toilets, household garbage cans (pools) and the like can be inhibited so as to be eliminated and the generation problem of odor is solved from the source of generating obnoxious gas (hydrogen sulfide, ammonia and the like). The household harmful microorganism elimination and deodorization solution has high harmful microorganism elimination and deodorization efficiency, high speed and strong adaptability. Meanwhile, toxic and harmful substances in the environments can be decomposed and converted into nontoxic and harmless substances.

The invention relates to a cucumber growing medium using pig manures and biogas residues as fertilizer sources, and a preparation method of the cucumber growing medium. The cucumber growing medium comprises biogas residues, vermiculite, turf and perlite in the weight part ratio of (2.5-3.5):3:(2.5-3.5):1. The biogas residues are from the biogas residues obtained after full fermentation in a large biogas digester, dried after solid-liquid separation and then crushed with a crusher; and Bacillus subtilis metabolites are absorbed on the turf. The defect that the traditional growing medium is predominant in turf is overcome, the usage of turf is reduced, the cost of the medium is reduced, and the ecological environment is protected; the pig manures and biogas residues are effectively utilized, the industry chain of biogas engineering is extended, and the environmental pollution caused by the pig manures and biogas residues is reduced; and meanwhile, the disease incidence rate of seedlings is effectively reduced, and the resistance against diseases of plants at the late growth stage is enhanced.

The invention provides a microecological preparation with bacillus subtilis fermenting astragalus membranaceus and an application thereof, wherein the microecological preparation is prepared through fermentation by means of a bacillus subtilis strain FM537 on a fermentation substrate containing astragalus membranaceus water extract liquid. The fermentation substrate comprises 15 g/L of tryptone, 15 g/L of glucose, 50 g/L of beef extract, 0.5 g/L of ammonium sulfate, 1 g/L of sodium chloride and 0.5 g/L of sodium dihydrogen phosphate. The fermentation substrate is prepared with distilled water, wherein pH is regulated to 7.2, and then according to volume ratio, 45-50% of the astragalus membranaceus water extract liquid is added, and finally high-pressure sterilization is carried out to produce the fermentation substrate. Through ultrasonic wave for assisting water extraction of the astragalus membranaceus, the extraction time is greatly reduced. Through selection of suitable fermentation temperature and fermentation time, synergistic effect of the astragalus membranaceus and the bacillus subtilis is improved. The microecological preparation is used as a livestock and poultry feed additive, and can improve animal production performance, digestion rate and body immunity.