212 results about "Surfactin" patented technology

The invention relates to an extracellular antibacterial lipopeptide of plant endophytic Bacillus subtilis Jaasedl and a separation and purification method. The distribution range of the molecular weight of the extracellular antibacterial lipopeptide is mainly between 1,000Da and 2,200Da. The extracellular antibacterial lipopeptide contains an Iturin homologue, a Fengycin homologue and a Surfactin-like Compound homologue. The extracellular antibacterial lipopeptide is a group of uncommon antibacterial lipopeptide mixture produced by a single bacterial strain. The separation and purification method of the extracellular antibacterial lipopeptide comprises the following steps of: after salting out to obtain the crude extract of the antibacterial lipopeptide by using ammonium sulfate from the fermentation liquor of the endophytic Bacillus subtilis Jaasedl, performing Sephedex G-25 molecular sieve chromatography, Cellulose DEAE-52 anion exchange chromatography and FPLC300SB-C18 column chromatography successively, wherein 34 to 37 min of collecting peak has bacteriostatic activity; detecting by Tricine-SDS-PAGE after concentrating; and achieving electrophoretically pure at only one strip to obtain pure extracellular antibacterial lipopeptide. The antibacterial lipopeptide has extremely high research and application value for the development of broad-spectrum antifungal medicaments, and has wide application prospect for natural quality protection of crops and pesticide residue reduction.

A process for producing Surfactin, comprising culturing a microorganism of the genus Bacillus in a liquid culture medium containing flour of beans such as soybean or an extract thereof as a nitrogen source and accumulating Surfactin in the culture broth, and a microorganism of the genus Bacillus which have an activity to produce a crude Surfactin in a concentration of from 8 to 50 g / L on culturing for 20 to 90 hours.

Surfactin is a biosurfactant produced by Bacillus subtilis and is a natural cycloaliphatic peptide having a ring structure made of 7 amino acids. The surfactin has various functions including anti-aging, anti-wrinkle, increasing skin penetration of cosmetic products (skin penetration agent), foaming agent, and emulsifier.

The invention relates to a method for increasing the yield of antibacterial peptide of bacillus subtilis through the overexpression of yerP gene, and belongs to the field of biotechnology. The method comprises the following steps of: constructing a gene overexpression vector pHCMC-YerP by adopting bacillus subtilis ATCC9943 as initial bacterial strain and escherichia coli cloning vector pHCMC04 as a framework, delivering the gene overexpression vector pHCMC-YerP into bacillus subtilis ATCC9943 through electrotransformation to achieve the overexpression of yerP gene, thereby constructing a high-yield antibacterial-peptide bacterial strain FMB45. Verified by high-performance liquid chromatography, the surfactin and fengycin producing capability of the improved bacterial strain is greatly improved.

The invention relates to a method increasing yield of Bacillus subtilis antimicrobial peptide by overexpression comA gene, belonging to the field of biotechnology. In the method, Bacillus subtilis ATCC9943 is used as an initial strain, and integrated vector pDK is used as a frame to construct a gene overexpression vector pDK-ComA; and by utilizing the homologous recombination principle, the comA gene is integrated into Bacillus subtilis ATCC9943 genome through a double exchange process, thereby constructing a high-yield antimicrobial peptide strain FMB27. The high efficiency liquid chromatography analysis proves that the surfactin / fengycin yield of the improved strain is greatly increased.