95 results about "Surfactin" patented technology

The invention discloses bacillus subtilis with high yield of surfactin, a related enzyme mutant and a preparation method of the bacillus subtilis, the bacillus subtilis is obtained by a gene modification method and can produce the surfactin with high yield, and the highest yield can reach 9.4 g / L; the invention also provides key enzyme mutants participating in surfactin synthesis in the bacillus subtilis, and the key enzyme mutants comprise a biotin carboxylase IdeHA mutant, a biotin carboxylase accBC mutant, an enoyl reductase FabI mutant, a malonyl transferase FabD mutant and a dihydrolipoyl dehydrogenase IPdU mutant. The key enzyme mutants comprise a biotin carboxylase IdeHA mutant, a biotin carboxylase accBC mutant, an enoyl reductase FabI mutant, a malonyl transferase FabD mutant and a dihydrolipoyl dehydrogenase IPdU mutant. The bacillus subtilis for high-yield surfactin and the preparation method thereof disclosed by the invention are beneficial to realizing industrial production and commercial application of the surfactin.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

The invention discloses gene-simplified bacillus subtilis for producing surfactin and application of the gene-simplified bacillus subtilis, and belongs to the technical field of gene engineering. According to the method disclosed by the invention, the variety of non-essential genes is determined by analyzing and identifying the bad performance influencing the fermentation production of the surfactin by the bacillus subtilis. Therefore, on the basis of the bacillus subtilis 168, a phosphopantetheinyl transferase (sfp) gene is firstly integrated, so that the surfactin production capacity of host bacteria is recovered; then, genes (clusters) in five aspects of a biological membrane synthesis pathway, a metabolic by-product pathway, spore generation, a protease system and flagellum generation are simplified. After the strain EX10 is simplified, the fermentation performance is optimized, and the fermentation yield reaches up to 11.2 g / L, which is 2.1 times higher than that of the original strain. Therefore, the genome-simplified strain has a good application prospect as a chassis cell for industrially and efficiently producing surfactin.

The invention discloses bacillus subtilis with high yield of specific surfactin and a preparation method and application thereof, the bacillus subtilis is obtained by a gene modification method and can produce high yield of C15 component surfactin, and the highest yield can reach 72.5%; the invention also provides an enzyme mutant participating in the production of the surfactin from the bacillus subtilis, wherein the enzyme mutant comprises carboxyl transferase accAD, biotin carboxylase IdeHA, biotin carboxylase accBC and fatty acid peroxygenase cypC; in addition, the invention also provides a preparation method and application of the bacillus subtilis. The bacillus subtilis for high-yield production of specific surfactin and the preparation method thereof provided by the invention are beneficial to increase of the yield of surfactin, especially industrial production and commercial application of C15 component surfactin.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The invention provides an application of a bacillus subtilis strain A45 in preventing and treating plant diseases or an application of the bacillus subtilis strain A45 in preparing a microbial agent for preventing and treating the plant diseases and the prepared microbial agent. The bacillus subtilis A45 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33289. According to the present invention, the mechanism of the liquid bacterial agent containing the bacillus subtilis A45 to efficiently inhibit the growth of the cucumber target leaf spot Corynespora cassicola is that the bacterial strain is encoded to synthesize the famogen, the purehydrazide acid, the subtilosin A and the surfactin synthesis gene cluster, the surfactin synthesis gene cluster is different from the synthesis gene of any known organism, and the highest sequence similarity is 82%. The invention further provides the application of the liquid bacterial agent containing the bacillus subtilis A45 to the preparation of the cucumber target leaf spot Corynespora cassicola, and the application of the liquid bacterial agent containing the bacillus subtilis A45 to the preparation of the cucumber target leaf spot Corynespora cassicola. Experiments show that the liquid fungicide containing the bacillus subtilis A45, provided by the invention, can be used for efficiently inhibiting the growth of the cucumber target leaf pathogen corynespora cassicola, and the prevention and treatment effect can be up to 80% when the liquid fungicide is used in a field.

The invention belongs to the technical field of biological extracts and cosmetics, and discloses a leukocyte extract composition as well as a preparation method and application thereof. The leukocyte extract composition comprises the following components in percentage by mass: 5-20% of a leukocyte extract; 3-10% of sodium surfactin; 10-20% of a humectant; and 50-82% of water. The preparation method of the leukocyte extract composition comprises the following preparation steps: adding the sodium surfactin into hot water at 50-80 DEG C for dissolving, then adding the humectant, homogenizing, stirring and uniformly mixing, cooling to room temperature, then adding the leukocyte extract, stirring and uniformly dissolving to obtain the leukocyte extract composition. According to the composition, a specific cyclic lipopeptide compound, namely, sodium surfactin, is adopted as a synergistic stable synergist of the leukocyte extract, so that the activity stability of the leukocyte extract can be remarkably improved, and better and more stable anti-aging, repairing and whitening effects can be achieved under the condition of smaller addition amount.

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

The invention provides a permeation promoting composition of anti-wrinkle and firming active substances and an anti-wrinkle and firming cosmetic. The permeation-promoting composition of the anti-wrinkle and firming active substances comprises the anti-wrinkle and firming active substances, wherein the anti-wrinkle and firming active substances comprise one or more of arginine / lysine polypeptide, tripeptide-1 copper and a sturgeon roe extract; the penetration promoting composition is prepared from 1, 2-pentanediol, inositol and sodium surfactin. According to the invention, the permeation of anti-wrinkle and firming active substances can be promoted, the addition amount of the anti-wrinkle and firming active substances in cosmetics can be reduced, and the production cost can be reduced. And the obtained anti-wrinkle and firming cosmetic can better inhibit the expression of MMP-9, promote the expression of type I collagen and reduce the wrinkle area.

The invention discloses a universal sample type nucleic acid releasing agent and application thereof. The nucleic acid releasing agent is prepared from the following components in molar concentration: 20 to 100 mM of Tris-HCl, 30 to 500 mM of sodium chloride, 30 to 200 mM of ammonium sulfate, 1 to 6 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 8 mM of a reducing agent, 0.1 to 0.2 percent of sodium dodecyl sarcosinate, 0.1 to 3 mM of Surfactin, 5 to 30 percent of Chelex-100 and 5 to 15 percent of trehalose. According to the method, various samples can be mildly split, impurities such as impure proteins and lipids can be effectively removed, the complexity of nucleic acid samples is reduced, and the method is seamlessly jointed with subsequent PCR (Polymerase Chain Reaction); the kit has double effects of sample preservation and splitting decomposition, can strongly inhibit various nuclease, and can ensure the long-term storage stability of samples.

A composition for caring for and / or making up keratin materials, comprising: (i) at least one surfactant selected from surfactins, their derivatives and mixtures thereof; (ii) at least one alkyl polyglucoside; and (iii) at least 12 wt.% of at least one fatty compound, relative to the total weight of the composition is provided. A non-therapeutic method for caring for and / or making up keratin materials is also provided.

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

This invention discloses a core-shell microneedle with both antibacterial and exudate-absorbing properties, and its preparation method, belonging to the field of microneedle drug delivery systems and acne treatment technology. The microneedle comprises: a core layer containing an exudate-absorbing component for absorbing exudate; and a shell layer covering the core layer, containing a microenvironment-responsive drug delivery unit and antibacterial nanoparticles loaded within the drug delivery unit. The drug delivery unit responds to the microenvironment of the acne lesion by releasing the antibacterial nanoparticles. This invention combines baicalein and surfactant into nanoparticles, loaded into a ROS-responsive shell layer formed by TSPBA and polyvinyl alcohol. Sodium polyglutamate is introduced into the core layer as an exudate-absorbing component. The core-shell microneedle is prepared by a two-step centrifugation method. This invention solves the problems of poor drug permeability, uncontrollable release, and exudate affecting treatment efficacy in traditional acne treatments. It achieves targeted drug release and exudate adsorption synergistic effects on acne lesions, improves drug bioavailability, reduces systemic side effects, and is suitable for the treatment of acne, especially inflammatory and purulent acne.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an agricultural bacteriostatic agent for preventing and treating Pseudomonas syringae and a preparation method of the agricultural bacteriostatic agent. The invention finds that surfactin produced by fermentation of bacillus subtilis has an inhibition effect on agricultural pathogenic bacteria pseudomonas syringae. The invention discloses an IC50 value of surfactin for inhibiting pseudomonas syringae as well as a formula and a preparation method of an agricultural bacteriostatic agent containing the surfactin. The invention provides a new way for preventing and treating pseudomonas syringae plant diseases.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

SKINCARE AND / OR MAKE-UP COMPOSITION FOR KERATINOUS MATERIALS The present invention relates to a skincare and / or makeup composition for keratinous materials, comprising: (i) at least one surfactant selected from surfactins, their derivatives, and mixtures thereof; (ii) at least 2% by weight of at least one wax with a melting point of at least 60°C, relative to the total weight of the composition; and (iii) at least one paste-like compound. The present invention also relates to a non-therapeutic method for skincare and / or makeup of keratinous materials. Figure for the abstract: none

The invention discloses a method for promoting fermentation of bacillus subtilis to produce surfactin by utilizing magnetoelectric coupling. The method comprises the following steps: adding magnesium ions into a fermentation system of bacillus subtilis, and carrying out continuous static magnetic field treatment and electrostatic field treatment on the fermentation system to obtain surfactin. According to the method disclosed by the invention, the content of the surfactin in the fermentation liquor is effectively increased through the synergistic effect of magnetoelectric coupling treatment and magnesium ions, a clean, effective and non-transgenic method is provided for increasing the yield of the surfactin, and the economic value of the application of the surfactin in food chemical industry, biological medicine or agriculture is increased.

An emulsion and a preparation method therefor. The emulsion comprises an emulsifier, glycerol, oil-phase active matter, pentaerythrityl tetraethylhexanoate, and water; the emulsifier is lipopeptide; the weight percentages of the lipopeptide and the glycerol are not less than 3%; the weight ratio of the oil-phase active matter to the pentaerythrityl tetraethylhexanoate is 1:(3-13); the ratio of the sum of the weight of the lipopeptide and the glycerol to the sum of the weight of the oil-phase active matter and the pentaerythrityl tetraethylhexanoate is 1:(3-10); and the ratio of the sum of the weight of the lipopeptide, the glycerol, the oil-phase active matter, and the pentaerythrityl tetraethylhexanoate to the weight of the water is 1:(1-4). In the emulsion, sodium surfactin is used in combination with the glycerol and the pentaerythrityl tetraethylhexanoate, so that the diffusion of the oil-phase active matter at an emulsion interface can be effectively inhibited, the oxidative degradation of the oil-phase active matter is reduced, and the effective action time of the oil-phase active matter in the emulsion is greatly prolonged.

The self-emulsifying composition, intended for administration onto the skin, contains a biosurfactant, a cosurfactant and an oil phase. The weight ratio of the bio surfactant, the cosurfactant and the oil phase is 0.01-96.99% by weight:0.01-96.99% by weight:3-70% by weight, respectively, where the bio surfactant is surfactin or its salt, and the cosurfactant is 2-(2-ethoxyethoxy)ethanol or cocamidopropyl betaine.

A method of producing an aqueous solution of zein suitable for forming edible coatings includes combining zein with an alcohol-free, non-corrosive amphipathic carrier solvent (e.g., [1,3]-propanediol), and adding a surfactant (e.g., surfactin) thereto, forming a Phase I solution. The surfactant may be non-ionic or anionic as determined by the type of zein being used. The Phase I solution is combined with a Phase II aqueous solution including primarily water without alcohol to provide an aqueous coating solution of zein. A plasticizer (e.g., polyethylene glycol) or fatty acid (e.g., lauric acid) may be added to the Phase II solution to enhance the zein coating. The resulting coating solution may then be applied on food, and dried, to form an edible coating.

To provide a cleansing cosmetic having low irritation, which is usable for sensitive skin.SOLUTION: The present invention relates to a cleansing cosmetic that includes (A) at least one component selected from surfactin and salts thereof: 0.3 mass% to 3 mass%, and (B) a surfactant other than component (A) and an anionic surfactant: 1 mass% to 6 mass% (wherein contents of components (A) to (B) are values based on a total mass of the cleansing cosmetic), the cleansing cosmetic being an O / D-type emulsion composition.SELECTED DRAWING: None

The invention discloses a composition containing 4-butylresorcinol as well as a preparation method and application of the composition, and belongs to the technical field of biological fermentation and cosmetic raw materials. The composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate, butanediol, isoprene glycol and water, wherein the composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate; wherein the bacillus subtilis lipopeptide sodium is prepared by fermenting bacillus subtilis H01 and has excellent surface activity, emulsifying property and antibacterial activity. The composition realizes efficient whitening by synergistically inhibiting skin tyrosinase activity and reducing melanin content, is good in component compatibility and high in stability, and can be widely applied to preparation of whitening and skin care products.

The invention provides a composition, a preparation method and application. Specifically, the carvacrol and the bacillus subtilis surfactin are combined, so that not only can the bacillus subtilis surfactin be used as an emulsifier of the carvacrol to increase the dispersion degree of the carvacrol in water, but also the carvacrol and the bacillus subtilis surfactin can achieve a synergistic effect to improve the inhibition effect on pathogenic bacteria. In addition, the composition provided by the embodiment of the invention can be gradually degraded under natural conditions, and is free of residues, free of chemical pollution, low in cost, high in biocompatibility and wide in application prospect.