61 results about "Surfactin" patented technology

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention relates to microbial colorant compositions and associated processes to produce the same. Microbial colorant compositions are produced by combining microbially derived pigment molecules and microbial biosurfactants. The pigment molecules are obtained either directly from a fermentation broth or by extracting a precursor and transforming it into a pigment using chemical or enzymatic processes. A separately produced biosurfactant, such as a rhamnolipid or surfactin, is added to the obtained pigment molecule to create a pre-dispersed formulation. The biosurfactant may be added at various steps of This approach improves pigment dispersion, solubility, and substrate compatibility, enabling stable aqueous formulations without synthetic additives. The resulting compositions are suitable for wide range of applications including in textiles, food, cosmetics, and polymer materials. Processes for producing and combining the pigment and biosurfactant are also disclosed, including variations in fermentation, extraction, and formulation stages.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The invention belongs to the technical field of biological extracts and cosmetics, and discloses a leukocyte extract composition as well as a preparation method and application thereof. The leukocyte extract composition comprises the following components in percentage by mass: 5-20% of a leukocyte extract; 3-10% of sodium surfactin; 10-20% of a humectant; and 50-82% of water. The preparation method of the leukocyte extract composition comprises the following preparation steps: adding the sodium surfactin into hot water at 50-80 DEG C for dissolving, then adding the humectant, homogenizing, stirring and uniformly mixing, cooling to room temperature, then adding the leukocyte extract, stirring and uniformly dissolving to obtain the leukocyte extract composition. According to the composition, a specific cyclic lipopeptide compound, namely, sodium surfactin, is adopted as a synergistic stable synergist of the leukocyte extract, so that the activity stability of the leukocyte extract can be remarkably improved, and better and more stable anti-aging, repairing and whitening effects can be achieved under the condition of smaller addition amount.

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

The application provides a nanoemulsion and a preparation method and application thereof. The total amount of raw materials for preparing the nanoemulsion is 100%, and the raw materials include the following components: 1-40% of a composite surfactant, 1-25% of an oil phase, 1-30% of a co-surfactant, and the balance of a water phase; wherein the mass of the composite surfactant is 100%, and the composite surfactant includes the following components: 5-25% of a biological surfactant, 35-65% of a fatty alcohol polyoxyethylene ether surfactant, 1-20% of dodecyl benzene sulfonic acid and / or sodium salt thereof, 1-20% of oleic acid, and 1-5% of citric acid; the biological surfactant includes one or more than two combinations of sophorolipid, rhamnolipid, trehalose lipid, surfactin, alkyl polyglycoside, phosphatidylcholine, amino acid lipid, saponin, phospholipid, or derivatives thereof; and the average particle size of the emulsion droplets in the nanoemulsion is 1.5-5 nm. The nanoemulsion has excellent recovery performance.

The invention discloses a universal sample type nucleic acid releasing agent and application thereof. The nucleic acid releasing agent is prepared from the following components in molar concentration: 20 to 100 mM of Tris-HCl, 30 to 500 mM of sodium chloride, 30 to 200 mM of ammonium sulfate, 1 to 6 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 8 mM of a reducing agent, 0.1 to 0.2 percent of sodium dodecyl sarcosinate, 0.1 to 3 mM of Surfactin, 5 to 30 percent of Chelex-100 and 5 to 15 percent of trehalose. According to the method, various samples can be mildly split, impurities such as impure proteins and lipids can be effectively removed, the complexity of nucleic acid samples is reduced, and the method is seamlessly jointed with subsequent PCR (Polymerase Chain Reaction); the kit has double effects of sample preservation and splitting decomposition, can strongly inhibit various nuclease, and can ensure the long-term storage stability of samples.

A composition for caring for and / or making up keratin materials, comprising: (i) at least one surfactant selected from surfactins, their derivatives and mixtures thereof; (ii) at least one alkyl polyglucoside; and (iii) at least 12 wt.% of at least one fatty compound, relative to the total weight of the composition is provided. A non-therapeutic method for caring for and / or making up keratin materials is also provided.

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

This invention discloses a core-shell microneedle with both antibacterial and exudate-absorbing properties, and its preparation method, belonging to the field of microneedle drug delivery systems and acne treatment technology. The microneedle comprises: a core layer containing an exudate-absorbing component for absorbing exudate; and a shell layer covering the core layer, containing a microenvironment-responsive drug delivery unit and antibacterial nanoparticles loaded within the drug delivery unit. The drug delivery unit responds to the microenvironment of the acne lesion by releasing the antibacterial nanoparticles. This invention combines baicalein and surfactant into nanoparticles, loaded into a ROS-responsive shell layer formed by TSPBA and polyvinyl alcohol. Sodium polyglutamate is introduced into the core layer as an exudate-absorbing component. The core-shell microneedle is prepared by a two-step centrifugation method. This invention solves the problems of poor drug permeability, uncontrollable release, and exudate affecting treatment efficacy in traditional acne treatments. It achieves targeted drug release and exudate adsorption synergistic effects on acne lesions, improves drug bioavailability, reduces systemic side effects, and is suitable for the treatment of acne, especially inflammatory and purulent acne.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an agricultural bacteriostatic agent for preventing and treating Pseudomonas syringae and a preparation method of the agricultural bacteriostatic agent. The invention finds that surfactin produced by fermentation of bacillus subtilis has an inhibition effect on agricultural pathogenic bacteria pseudomonas syringae. The invention discloses an IC50 value of surfactin for inhibiting pseudomonas syringae as well as a formula and a preparation method of an agricultural bacteriostatic agent containing the surfactin. The invention provides a new way for preventing and treating pseudomonas syringae plant diseases.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

SKINCARE AND / OR MAKE-UP COMPOSITION FOR KERATINOUS MATERIALS The present invention relates to a skincare and / or makeup composition for keratinous materials, comprising: (i) at least one surfactant selected from surfactins, their derivatives, and mixtures thereof; (ii) at least 2% by weight of at least one wax with a melting point of at least 60°C, relative to the total weight of the composition; and (iii) at least one paste-like compound. The present invention also relates to a non-therapeutic method for skincare and / or makeup of keratinous materials. Figure for the abstract: none

A method of producing an aqueous solution of zein suitable for forming edible coatings includes combining zein with an alcohol-free, non-corrosive amphipathic carrier solvent (e.g., [1,3]-propanediol), and adding a surfactant (e.g., surfactin) thereto, forming a Phase I solution. The surfactant may be non-ionic or anionic as determined by the type of zein being used. The Phase I solution is combined with a Phase II aqueous solution including primarily water without alcohol to provide an aqueous coating solution of zein. A plasticizer (e.g., polyethylene glycol) or fatty acid (e.g., lauric acid) may be added to the Phase II solution to enhance the zein coating. The resulting coating solution may then be applied on food, and dried, to form an edible coating.

To provide a cleansing cosmetic having low irritation, which is usable for sensitive skin.SOLUTION: The present invention relates to a cleansing cosmetic that includes (A) at least one component selected from surfactin and salts thereof: 0.3 mass% to 3 mass%, and (B) a surfactant other than component (A) and an anionic surfactant: 1 mass% to 6 mass% (wherein contents of components (A) to (B) are values based on a total mass of the cleansing cosmetic), the cleansing cosmetic being an O / D-type emulsion composition.SELECTED DRAWING: None

The invention discloses a composition containing 4-butylresorcinol as well as a preparation method and application of the composition, and belongs to the technical field of biological fermentation and cosmetic raw materials. The composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate, butanediol, isoprene glycol and water, wherein the composition is prepared from sodium surfactin, sodium stearoyl glutamate, 4-butylresorcinol, polyglycerol-4 oleate; wherein the bacillus subtilis lipopeptide sodium is prepared by fermenting bacillus subtilis H01 and has excellent surface activity, emulsifying property and antibacterial activity. The composition realizes efficient whitening by synergistically inhibiting skin tyrosinase activity and reducing melanin content, is good in component compatibility and high in stability, and can be widely applied to preparation of whitening and skin care products.

The invention provides a composition, a preparation method and application. Specifically, the carvacrol and the bacillus subtilis surfactin are combined, so that not only can the bacillus subtilis surfactin be used as an emulsifier of the carvacrol to increase the dispersion degree of the carvacrol in water, but also the carvacrol and the bacillus subtilis surfactin can achieve a synergistic effect to improve the inhibition effect on pathogenic bacteria. In addition, the composition provided by the embodiment of the invention can be gradually degraded under natural conditions, and is free of residues, free of chemical pollution, low in cost, high in biocompatibility and wide in application prospect.

The present application relates to the field of genetic engineering and bioengineering, and particularly relates to a genetically engineered surfactin-producing bacterium and application thereof.The genome of the genetically engineered bacterium comprises the following: an expression frame of T7 RNA polymerase; an expression frame of a surfactin synthesis gene cluster srfAABCD; and an expression frame of a long-chain fatty acid-coenzyme A ligase gene lcfA; the expression frame of the surfactin synthesis gene cluster srfAABCD and the expression frame of the long-chain fatty acid-coenzyme A ligase gene lcfA further comprise a T7 promoter.The present application realizes efficient synthesis of surfactin by Bacillus subtilis, and the final shake flask yield reaches more than 19.3 g / L, which has great industrial application potential.

The invention relates to the field of prevention and treatment of gas hydrates in deepwater oil and gas gathering and transportation pipelines, in particular to a bacillus subtilis lipopeptide sodium hydrate polymerization inhibitor and application thereof. The bacillus subtilis lipopeptide sodium has a result general formula as shown in a formula I, and R1 in the formula I is a C12-C15 saturated fatty acid chain. The polymerization inhibitor can enable generated hydrate particles to form hydrate slurry, and the hydrate slurry flows in a pipeline along with fluid and does not aggregate into large blocks or deposit on a pipe wall. The method is suitable for gas-water two-phase and oil-gas-water three-phase coexistence systems, the effective concentration is as low as 0.01 wt% (based on the mass of system water), and the method has the characteristics of wide applicability, low cost and environmental friendliness.

This invention provides Bacillus subtilis ( Bacillus subtle The application of strain A45 in the control of plant diseases, its application in the preparation of microbial agents for the control of plant diseases, and the prepared microbial agents. Bacillus subtilis A45 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33289. The liquid microbial agent containing Bacillus subtilis A45 provided by this invention effectively inhibits the cucumber target spot pathogen, *Clostridium multiflorum* (…). Corynespora cassicola The growth mechanism of this strain lies in its encoding a gene cluster for the synthesis of phytosalicylic acid, purchemin, subtilosin A, and surfactant. Among these, the surfactant synthesis gene cluster is unique to this strain, exhibiting a maximum sequence similarity of 82%. Experiments show that the liquid inoculant containing Bacillus subtilis A45 provided by this invention can effectively inhibit the growth of *Clostridium multiflorum*, the pathogen causing target spot disease in cucumbers. Corynespora cashew nut The growth of ) can be controlled by field application with a prevention and control effect of up to 80%.

The invention discloses a TE enzyme mutant with improved activity and application thereof, and belongs to the technical field of biology. Schrodinger molecular docking is combined with fixed-point saturation mutation to establish a mutant library, Rosetta is used for screening the mutant library, searching a target point and carrying out single-point mutation and combined mutation, the activity of thioesterase is improved, the synthesis efficiency of Surfactin is further improved, and a production strain with better fermentation performance in industrial production is obtained. Through verification, mutations comprise A113W, A113G, K122H, K192D and a combination of the A113W, the A113G, the K122H and the K192D, fermentation verification shows that the yield of the Surfactin is increased by 41.2% to the maximum compared with that of a wild type and reaches 9.6 g / L, thioesterase with higher activity and a production strain with better fermentation performance in industrial production are obtained, and powerful support is provided for efficient production of the Surfactin.

To provide a cleansing cosmetic that exhibits excellent post-cleansing film feel, protective feel, and moist feel.SOLUTION: The present invention relates to a cleansing cosmetic that includes (A) at least one component selected from surfactin and salts thereof: 0.3 mass% to 10 mass%, (B) a surfactant other than component (A): 1 mass% to 10 mass%, and (C) an oil: 20 mass% to 80 mass% (wherein contents of components (A) to (C) are values based on a total mass of the cleansing cosmetic), a mass ratio ((C) / (B)) of component (C) to component (B) being 3.0 to 75, the cleansing cosmetic being an O / D-type emulsion composition.SELECTED DRAWING: None

The invention relates to the field of oral disease prevention and treatment, in particular to oral ulcer gargle containing sodium surfactin, which comprises polyvinylpyrrolidone, carboxymethyl cellulose sodium, glycerol, N-acetamido glucose, sorbitol, saccharin sodium, lactic acid, sodium surfactin and purified water. The sodium bacillus subtilis lipopeptide is combined with pathogenic microorganisms and potential cancerous cells through electrostatic attraction, precise killing is achieved, PVP and CMC-Na form a physical protection layer on the ulcer surface to isolate irritants and relieve pain, sodium bacillus subtilis lipopeptide and lactic acid have a synergistic effect, cancerogenic substances such as arecoline in the oral cavity can be wrapped or degraded, the cancerous risk is reduced, and the cancerous effect is improved. And the components are physiologically inert, have good biocompatibility and do not irritate oral mucosa.

The invention provides a fermentation production method rich in C15 Surfactin. The fermentation production method comprises the following steps: inoculating bacillus subtilis into a seed culture medium for culturing; inoculating the cultured seed solution into a fermentation culture medium containing amino acid for fermentation; wherein L-leucine, L-glutamic acid or L-isoleucine is adopted as the amino acid. According to the method, the L-leucine, the L-glutamic acid or the L-isoleucine is added to induce the bacillus subtilis to efficiently synthesize the C15-surfactin, so that the total yield of the surfactin is increased to 30 g / L, the proportion of the C15-surfactin is increased to 68%, and the method is remarkably superior to a traditional fermentation process. In addition, the fermentation product shows better bacteriostatic activity and biological safety, and shows better application prospects.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus subtilis KG25 and application thereof. The bacillus subtilis KG25 is preserved in the China Center for Type Culture Collection on September 1, 2025, the classification name of the bacillus subtilis KG25 is bacillus subtilis KG25, and the preservation number of the bacillus subtilis KG25 is CCTCC (China Center for Type Culture Collection) NO: M 20251927. The bacillus subtilis KG25 provided by the invention can be used for synthesizing surfactin with relatively high content, has a function of inhibiting enzymatic browning, and has a very remarkable fresh-keeping function on fresh-cut fruits and vegetables. The composite preservative containing the bacillus subtilis KG25 fermentation liquor provided by the invention is used for soaking fresh-cut fruits and vegetables, so that the normal-temperature storage period can be prolonged to 7 days, and the composite preservative has a wide application prospect in the field of fruit and vegetable preservation.

The application belongs to the technical field of fresh-keeping materials, and particularly relates to a Surfactin composite film, a preparation method thereof and application of the Surfactin composite film in fruit and vegetable fresh-keeping, the preparation method comprising the following steps: preparing polyvinyl alcohol and chitosan into solutions respectively, mixing the two solutions, adding glycerol and citric acid, heating and stirring to make the system uniform, and obtaining a mixed solution; adding Surfactin and mixing uniformly, then casting and drying to obtain the Surfactin composite film. The composite film has good antibacterial performance and ultraviolet blocking performance, can effectively delay water loss of fresh-cut apples and inhibit quality deterioration, and has important application value and prospect in fruit and vegetable fresh-keeping packaging.

The invention belongs to the technical field of fertilizers, and particularly relates to a Surfactin-containing organic water-soluble fertilizer for improving saline-alkali stress resistance of wheat seeds, the organic water-soluble fertilizer comprises organic matters, macronutrient elements, micronutrient elements, functional components and Surfactin; and the content of Surfactin in the fertilizer is 1000 to 3000 mg / kg. The Surfactin-containing organic water-soluble fertilizer has the advantages of no toxicity, no pollution, simple preparation process, convenience in use and good stability, provides a theoretical basis for the research on the promotion of the Surfactin-containing organic water-soluble fertilizer on seed germination under salt stress, and has a wide market application value in the development and planting of saline-alkali soil crops.