34 results about "Drug permeability" patented technology

The invention relates to the field of nano materials, in particular to a chitosan and recombinant collagen composite nano material as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing chitosan with an acid solution to obtain a chitosan acid solution; mixing the recombinant collagen with a buffer solution to obtain a recombinant collagen buffer solution; dropwise adding the chitosan acid solution into the recombinant collagen buffer solution, so that the chitosan particles and the recombinant collagen particles are self-assembled into composite nanoparticles; the particle size of the composite nanoparticles is smaller than or equal to 300 nm. The chitosan acid solution and the recombinant collagen buffer solution form a stable nanoparticle structure through the self-assembly effect of positive and negative charges, and the problem of toxicity introduced by traditional chemical crosslinking is avoided. The particle size of the composite nanoparticles is less than or equal to 300nm, so that the transdermal efficiency of the collagen is improved; due to the structural characteristics of the nanoparticles, the permeability and the local bioavailability of the medicine are remarkably improved.

The invention belongs to the technical field of pharmaceutical preparations, and relates to a compound I patch composition, a compound I patch and a preparation method thereof. The patch composition comprises the following components in parts by weight: 0.5-12 parts of a pharmaceutical active component, 10-30 parts of a penetration enhancer, 15-56 parts of a crystal inhibitor and 35-57 parts of an acrylic acid pressure-sensitive adhesive, the patch composition also comprises solvent dimethyl sulfoxide and absolute ethyl alcohol, and the residual quantity of the dimethyl sulfoxide is not less than 2.0%; wherein the active pharmaceutical ingredient is a compound I, and the structural formula of the compound I is shown in the specification. According to the patch composition containing the compound I, the medicine stability is high, medicine crystallization and impurity generation are effectively avoided or reduced, and the patch containing the compound I has higher stability and safety; the patch composition containing the compound I has more appropriate drug permeability, so that the patch containing the compound I has higher drug effectiveness.

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine spray for treating rhinitis and a preparation method thereof.The traditional Chinese medicine spray is prepared from flos magnoliae liliflorae, fructus xanthii, herba ephedrae, fructus forsythiae, flos lonicerae, radix angelicae, herba asari, menthol, herba centipedae and herba menthae. Refining to obtain a fluid extract; and mixing the fluid extract with a penetration enhancer consisting of aminoketone and menthol, a sodium hyaluronate mucous membrane repairing agent, a pH regulator, an isoosmotic adjusting agent and a preservative to prepare spray liquid medicine, and finally performing sterile filtration and filling. Through efficient extraction and synergism of composite auxiliary materials, the permeability and bioavailability of the medicine are remarkably improved, and the symptoms such as nasal obstruction and running nose can be quickly relieved; meanwhile, the anti-inflammatory function and the function of actively repairing the damaged nasal mucosa are achieved, and the recurrence rate is reduced from the source; the pH and osmotic pressure of the preparation are precisely adjusted, the use is comfortable, and the quality is stable and controllable.

To provide a manufacturing method of rubber gloves having a good balance of other functions (e.g. electric conductivity, flexibility, strength, etc.) in addition to chemical deterioration resistance and chemical permeation resistance and to provide a composition for manufacturing rubber products and the rubber gloves.SOLUTION: A manufacturing method of rubber gloves includes the steps of: mixing rubber materials and carbon nanotubes to produce a rubber compound; dissolving the rubber compound with an organic solvent to produce a rubber solution; immersing a glove mold in the rubber solution to form a rubber composition; and vulcanizing the rubber composition.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of biomedicine, and discloses a soluble microneedle for treating hyperplastic scars as well as a preparation method and application of the soluble microneedle. The preparation method comprises the following steps: modifying hyaluronic acid to obtain sulfonated hyaluronic acid, blending the sulfonated hyaluronic acid with a natural penetration enhancer d-borneol with anti-inflammatory activity, carrying out rotary evaporation and freeze-drying to obtain a sulfonated hyaluronic acid-d-borneol solid dispersion, and loading the solid dispersion into a microneedle to obtain the soluble microneedle with the hyperplastic scar treatment function. The preparation method is simple and easy to operate, and large-scale production can be realized. According to the preparation method, the sulfonated hyaluronic acid and the d-borneol are effectively combined, the bioavailability and biocompatibility of the d-borneol are improved, and the d-borneol is loaded into the microneedle, so that the anti-inflammatory and fibroblast migration inhibition capability of the solid dispersion is maximized, and meanwhile, the anti-inflammatory and fibroblast migration inhibition capability of the solid dispersion can be further improved under the dual permeation promotion of the microneedle and the d-borneol. And the aim of deeply delivering the medicine is fulfilled by penetrating dense scar tissues. The problems that in a traditional scar treatment method, medicine permeability is poor, and the treatment effect is limited are solved, a safe and efficient novel transdermal drug delivery system is provided for treating hyperplastic scars, and wide clinical application prospects and market value are achieved.

The invention discloses ointment for relieving swelling and ostealgia and promoting tissue regeneration and a preparation method thereof, and relates to the field of traditional Chinese medicine, and the ointment is prepared from the following raw materials: catechu, cape jasmine, trogopterus dung, kadsura root-bark, madder, dragon's blood, rheum officinale, frankincense and myrrh. According to the swelling-eliminating ostealgia granulation-promoting ointment and the preparation method thereof, by scientifically compounding a plurality of traditional Chinese medicinal materials capable of promoting blood circulation to remove blood stasis and promoting granulation to relieve pain and combining with an accurate grinding process and an optimized proportion of a honey matrix, the medicine permeability and bioavailability of the ointment are remarkably improved; the traditional Chinese medicine composition has multiple effects of relieving swelling and pain, promoting blood circulation to remove blood stasis, relaxing tendons and activating collaterals, and promoting tissue regeneration and strengthening muscles and bones, and particularly has a more remarkable curative effect on edema pain caused by rheumatism, rheumatoid, gout and various osteoarthropathy and diseases such as early bone fracture, bone fracture and fracture.

A self-powered intelligent adhesive bandage capable of detecting temperature in real time and dosing according to needs is applied to detection and treatment of skin wound infection and can detect the temperature of a wound and surrounding skin in real time and conduct dosing according to needs. According to the intelligent adhesive bandage, the piezoelectric nanometer generator is combined, mechanical energy generated in the daily activity process of a wearer can be converted into electric energy, and the electric energy is used for driving the whole intelligent adhesive bandage to operate. The two temperature measuring units are used for detecting the temperature of the wound and the surrounding skin at the same time, the temperature difference value serves as a relative index for judging whether the wound is infected or not, and the influence of environmental factors on detection is reduced. A three-electrode structure is adopted, on-demand drug delivery is achieved through iontophoresis, and high drug permeability, dosage controllability and drug delivery uniformity are all considered. After the intelligent adhesive bandage is used for treating a mouse with wound infection for three days, the wound recovery rate of the mouse reaches 90.3%, and the realization of temperature detection and on-demand administration is proved. The intelligent adhesive bandage has a good application prospect in detection and treatment of wound infection.

The invention provides a microsphere composition for a contraceptive microneedle and the contraceptive microneedle containing the composition. The microsphere composition for the contraceptive microneedle comprises one selected from the group consisting of sustained-release estrogen microspheres, sustained-release progesterone microspheres and mixtures thereof, and the sustained-release estrogen microspheres comprise 400-600 parts by weight of biodegradable polymer per 100 parts by weight of estrogen, every 100 parts by weight of progesterone and hydroxypropyl-beta-cyclodextrin (HP [beta] CD) in the sustained-release progesterone microspheres comprise 700-900 parts by weight of a biodegradable polymer, and the molar ratio of the progesterone to the hydroxypropyl-beta-cyclodextrin is (1: 2)-(1: 3). The microsphere composition for a contraceptive microneedle according to the present invention can stably encapsulate a drug, exhibits a sustained-release drug effect for at least one week, and has a particle size (Dv90) suitable for a microneedle. According to the contraceptive microneedle or the contraceptive microneedle patch, administration is convenient, the contraceptive microneedle or the contraceptive microneedle patch is used once a week, the transdermal medicine permeability is good, the short attachment time is allowed, and the medicine is slowly released for at least one week.

This invention discloses a core-shell microneedle with both antibacterial and exudate-absorbing properties, and its preparation method, belonging to the field of microneedle drug delivery systems and acne treatment technology. The microneedle comprises: a core layer containing an exudate-absorbing component for absorbing exudate; and a shell layer covering the core layer, containing a microenvironment-responsive drug delivery unit and antibacterial nanoparticles loaded within the drug delivery unit. The drug delivery unit responds to the microenvironment of the acne lesion by releasing the antibacterial nanoparticles. This invention combines baicalein and surfactant into nanoparticles, loaded into a ROS-responsive shell layer formed by TSPBA and polyvinyl alcohol. Sodium polyglutamate is introduced into the core layer as an exudate-absorbing component. The core-shell microneedle is prepared by a two-step centrifugation method. This invention solves the problems of poor drug permeability, uncontrollable release, and exudate affecting treatment efficacy in traditional acne treatments. It achieves targeted drug release and exudate adsorption synergistic effects on acne lesions, improves drug bioavailability, reduces systemic side effects, and is suitable for the treatment of acne, especially inflammatory and purulent acne.

The invention discloses a gel plaster for treating chronic soft tissue injury and a preparation method thereof, and relates to the technical field of traditional Chinese medicine preparations, the gel plaster comprises a backing material, a covering film, a traditional Chinese medicine extract and a matrix; the traditional Chinese medicine extract is prepared from the following raw medicinal materials: radix angelicae pubescentis, ligusticum wallichii, common vladimiria root, fennel, cinnamon, frankincense, myrrh, radix aconiti preparata, prepared kusnezoff monkshood root, dragon's blood and borneol; the matrix comprises the following components: a humectant, a thickening agent, a framework material, a cross-linking agent, a pH regulator and an adhesive. As a drug delivery carrier, the nano-emulsion gel paste disclosed by the invention greatly improves the drug permeability, increases the absorption capacity, provides an efficient and safe novel transdermal drug delivery scheme for treating chronic soft tissue injury, and solves the problem of low viscosity in a skin application process.

To provide implantable devices that can deliver drugs to different various drugs by an effective release rate.SOLUTION: Implantable drug delivery devices include a housing having a closed drug reservoir lumen bounded by a first wall structure and a hydrophilic second wall structure, and a drug contained in the drug reservoir lumen, wherein the first wall structure is impermeable to the drug and the second wall structure is permeable to the drug. Methods of providing controlled release of drug to a patient include deploying a drug delivery device in the patient releasing a drug from the drug reservoir lumen via diffusion through the second wall structure.SELECTED DRAWING: Figure 8A

The invention relates to the technical field of drug carriers, in particular to a paste matrix, a preparation method thereof, a paste drug carrier and application. The preparation method of the paste matrix comprises the following step: carrying out thermal polymerization reaction on a high-molecular compound containing an epoxy group, a nucleophilic reagent and a phenolic acid compound in a deep eutectic solvent to obtain the paste matrix. The pasty matrix and the pasty drug carrier are high in adhesion in a wet environment, good in biocompatibility, good in drug permeability and relatively simple in preparation process.

The invention belongs to the technical field of drug synthesis, and provides an imatinib derivative and a preparation method and application thereof, the imatinib derivative provided by the invention has a new structure, and can reduce tumor interstitial fluid pressure, enhance drug permeability and improve drug curative effect. The specific preparation method comprises the following steps: mixing 4-mercaptobenzoic acid, N, N-dimethylformamide, N-hydroxysuccinimide and an N, N '-dicyclohexylcarbodiimide solution to obtain an activation system; and mixing the active system with imatinib amine for reaction to obtain the imatinib derivative. According to the preparation method, the synthesis reaction process is simple and easy to control, and the price is low.

The present application relates to a transdermal patch containing colchicine and a preparation method and use thereof. The transdermal patch comprises a matrix layer, wherein the matrix layer comprises an active ingredient colchicine, a hot melt pressure sensitive adhesive and optionally other pharmaceutically acceptable adjuvants, wherein the content of the colchicine is 0.1% to 5%, the content of the hot melt pressure sensitive adhesive is 1.9% to 99.9%, and the content of the other pharmaceutically acceptable adjuvants is 0% to 98% based on the total weight of the matrix layer. The transdermal patch containing colchicine of the present application has no local irritation during and after application, no gastrointestinal side effects, high patient compliance, strong drug permeability, good therapeutic effect, and can be used for preventing and / or treating gout and / or pericarditis.

The invention discloses application of lipoic acid trisulfide, and belongs to the technical field of medicines. The invention discloses application of lipoic acid trisulfide or salt, isomer and derivative thereof as a drug penetration enhancer or / and an efflux inhibitor. The invention also discloses a medicinal preparation which comprises a medicine penetration enhancer or / and an efflux inhibitor, and the medicine penetration enhancer or / and the efflux inhibitor is lipoic acid trisulfide or salt and isomer thereof. The lipoic acid trisulfide is scientific in design and ingenious in conception, it is found for the first time that the lipoic acid trisulfide or the salt, isomer and derivative thereof not only can improve the drug permeability, but also shows an excellent drug excretion inhibition effect, and has a dual regulation function. As a drug penetration enhancer or / and an excretion inhibitor, the compound can significantly improve the bioavailability of drugs.

The present invention relates to a method using low intensity pulsed ultrasound (LIPUS) in order to improve drug delivery efficiency in a hypoxic tumor microenvironment (TME) of cholangiocarcinoma (CCA), and more specifically, to a method for confirming the drug permeability and cancer cell apoptosis of cholangiocarcinoma through the irradiation of low intensity pulsed ultrasound (LIPUS) under a tumor microenvironment, and a combination regimen, of gemcitabine and cisplatin with low-intensity pulsed ultrasound, for inhibiting cholangiocarcinoma growth. To this end, provided is the method for confirming the drug permeability and cancer cell apoptosis of cholangiocarcinoma through the irradiation of low intensity pulsed ultrasound (LIPUS) under a tumor microenvironment, the method comprising: a step (S100) for administering a drug and a fluorescent material to cholangiocarcinoma (CCA) under a tumor microenvironment; a step (S120) for allowing a predetermined time to elapse; a step (S140) for irradiating the cholangiocarcinoma (CCA) low intensity pulsed ultrasound (LIPUS); a step (S160) for acquiring a 3D image by performing tissue-clearing on the cholangiocarcinoma (CCA); and a step (S180) for confirming the in vivo drug permeability of a drug and a fluorescent material from the 3D image.

The invention discloses a traditional Chinese medicine external application composition prescription and a combined patch for treating lumbago, and the traditional Chinese medicine external application composition prescription comprises the following raw materials by weight: 1-5 parts of garden balsam stem, 0.5-2.5 parts of lycopodium clavatum, 0.5-2.5 parts of Chinese violet, 0.2-2 parts of clematis root, 0.2-2 parts of frankincense, 0.2-2 parts of myrrh, 0.2-2 parts of achyranthes root, 0.2-2 parts of ligusticum wallichii, 0.2-2 parts of cassia twig, 0.2-2 parts of ground beetle, 0.2-2 parts of notopterygium root, and 0.2-2 parts of radix angelicae pubescentis. The traditional Chinese medicine composition is prepared from 0.2-2 parts of radix angelicae sinensis, 0.2-1 part of flos carthami, 0.2-2 parts of radix aconiti preparata, 0.2-2 parts of radix aconiti kusnezoffii preparata, 0.2-2 parts of radix salviae miltiorrhizae, 0.2-2 parts of cortex eucommiae and 0.2-2 parts of radix glycyrrhizae. The medicinal composition provided by the invention is more comprehensive than other similar components for treating lumbago, has the main effects of dispelling wind and eliminating dampness, and promoting blood circulation to remove meridian obstruction, also has the effects of tonifying liver and kidney, and is suitable for arthralgia, soreness and weakness of waist and knees and other symptoms caused by wind-cold damp evil and stagnation of qi and blood stasis. Medicine permeability is improved and accelerated by means of the heating function, blood circulation is promoted, blood stasis is removed, channel warming and cold dispelling are facilitated, meanwhile, traditional Chinese medicine safety is guaranteed to a certain extent through traditional Chinese medicine sterilizing liquid, and allergy is reduced.

The application discloses a hot compress magnetotherapy glove with drug penetration assistance, and belongs to the technical field of arthritis treatment gloves.The hot compress magnetotherapy glove comprises a main body, a hot compress magnetotherapy mechanism is arranged in the main body, the hot compress magnetotherapy mechanism comprises a bracelet controller, one end of the bracelet controller is connected with a peristaltic pump through a wire, one side of the peristaltic pump is connected with a medicine bag through a catheter, both sides of the peristaltic pump are connected with medicine guide tubes, the medicine guide tubes are attached to the inner wall of a glove cavity, and one end of the glove cavity is connected with a finger cavity.The hot compress magnetotherapy glove generates ultrasonic waves of a specific frequency through a regulated micro ultrasonic transducer; the mechanical effect of the ultrasonic waves makes the cell gap of the cutin layer of the skin larger, the thermal effect promotes blood circulation of the skin, the cavitation effect forms micro cavities on the surface of the skin, and the three auxiliary combinations of the neodymium-iron-boron permanent magnet and the heating layer enhance the drug permeability, so that the drug molecules diffuse and absorb to the joint tissue, and the use of the patient is facilitated.

The invention discloses a preparation method of a hyaluronic acid modified lactoferrin nano transmission system, which is specifically implemented according to the following steps: step 1, weighing lactoferrin, dissolving the lactoferrin in water, and stirring on a magnetic stirrer; step 2, weighing paclitaxel, dissolving paclitaxel in an absolute ethyl alcohol solution, slowly adding into the LF solution, magnetically stirring, carrying out ultrasonic emulsification, and then filtering and freeze-drying; step 3, preparing HA-LF (at) PTX; and step 4, carrying out freeze-drying on the prepared HA-LF (at) PTX to obtain HA-LF (at) PTX powder. The invention also discloses application of the lactoferrin nano delivery system prepared by the method in treatment of bladder cancer. According to the invention, the problems of insufficient targeting and poor drug permeability in the existing bladder perfusion therapy are solved.

Microphysiological platforms are configured for measuring kinetic rates relating to drug absorption. These kinetic rates include GI region-specific kinetic rates of drug absorption. The kinetic rates include bioavailability of the drug over time in various tissues. The kinetic rates include drug permeability in various tissues. The kinetic rates include measurements of microbial transformations that occur. The kinetic rates include measured effects of gastric emptying and transit times. The kinetic rates include measurements of fasting and fed states for various tissues.

The invention relates to the technical field of biomedicine, and aims to solve the problems of poor drug permeability, drug resistance, drug toxicity, high surgical risk, infection recurrence, donor tissue shortage and the like of prevention and treatment means of fungal keratitis. The invention provides application of a metabolic marker in preparation of a product for diagnosing, preventing or treating fungal keratitis and a screening method of the metabolic marker. The metabolic marker is at least one of adenosine, adenosine monophosphate and uric acid. In fungal cornea, adenosine and adenosine monophosphate are reduced, and uric acid is increased. According to the invention, three differential metabolites are screened based on the combination of non-targeted metabonomics and targeted mass spectrometry detection, and the provided purine metabolic pathway regulation and the level of the three metabolites can be used for diagnosis of fungal keratitis patients and research and development of new therapeutic targets.

The present invention relates to a gradient microfluidic chip for drug screening, a drug screening method using same, and a drug screening method for preventing or treating Fabry disease by using same. More specifically, the present invention relates to a gradient microfluidic chip having a structure capable of culturing and differentiating organoids at four constant drug concentration gradients together with continuous medium flow. When the gradient microfluidic chip for drug screening according to the present invention is used, the physiological function of organoids is improved by inducing the vascularization and differentiation of the organoids, thereby enabling reproduction of an organ at a human body level better than existing organoids, nutrients and various concentrations of a drug are smoothly supplied, and drug permeability is increased. Thus, a concentration-dependent therapeutic effect of a drug can be confirmed with high drug sensitivity, and therefore the gradient microfluidic chip enables rapid and effective drug screening.

The invention belongs to the technical field of biological medicines, and particularly relates to a spermidine functionalized Janus hydrogel microneedle system as well as a preparation method and application thereof. The Janus hydrogel microneedle system is composed of a mucous membrane adhesion layer (HTSO hydrogel) and an anti-adhesion layer (GSC hydrogel), the mucous membrane adhesion layer is formed by dynamic Schiff base crosslinking of hyaluronic acid-spermidine conjugate and oxidized dextran loaded with lidocaine, and the anti-adhesion layer is composite hydrogel composed of carboxymethyl chitosan, gelatin and silk fibroin. The Janus hydrogel microneedle system provided by the invention can directly deliver a drug to mucous membrane tissues, so that the permeability of the drug is improved, the compliance of a patient is improved through painless local drug delivery, and the Janus hydrogel microneedle system shows lasting adhesion in an oral environment, effectively inhibits ferroptosis activity and active oxygen, remodels a local immune microenvironment and has a good application prospect. In-vivo angiogenesis and epithelium regeneration are promoted.

The utility model provides an alkaline soil improvement and remediation device, belongs to the technical field of soil improvement, and solves the technical problems that the existing medicine can only be simply discharged to the ground, so that the medicine permeability is poor, and the treatment effect is influenced. An alkaline soil improvement and remediation device comprises a remediation box, a pair of driving wheels are arranged at the bottom of the remediation box, a pair of servo motors are fixed in the bottom of the remediation box, an output shaft of each servo motor is coaxially and fixedly connected with the corresponding driving wheel, a liquid mixing cavity is formed in the remediation box, and a driving disc is rotationally installed in the liquid mixing cavity; a stirring rod is fixed to the disc face of the driving disc, a pair of transmission wheels is arranged at the bottom of the repairing box, and a transmission assembly is arranged in the repairing box. According to the device, the soil can be overturned and loosened in the improvement and remediation process, so that the granular structure of the soil is recovered, and the permeation effect of the soil on drugs and water and the air permeability of the soil are enhanced.

Provided is a drug delivery device with which it is possible to efficiently improve drug permeability and which can be easily arranged on a curved surface of epithelial tissue. This drug delivery device has a base material sheet extending in a first direction, a drug-containing part containing a drug, and a plurality of electrodes extending in a direction that intersects the first direction and is parallel to the base material sheet, the plurality of electrodes being arranged at intervals in the first direction.

The invention discloses targeted nano-liposomes (SPL NPs) capable of releasing nitric oxide (NO) through ultrasonic triggering and application of the targeted nano-liposomes (SPL NPs) combined with a PD-L1 antibody in pancreatic cancer treatment. The nano-liposome is formed by self-assembly of DSPE-PEG2000-SNO (which is used as a NO prodrug), pyropheophorbide acid (PPAL (which is used as a sound-sensitive agent) and DSPE-PEG2000-LFC131 (which is used as a pancreatic cancer targeting ligand), and has good stability and targeting property. Under an ultrasonic irradiation condition, the nano system can generate active oxygen at a tumor part through the sonodynamic effect of PPAL, and can induce obvious immunogenic cell death. In addition, quick release of NO of an NO prodrug DSPE-PEG2000-SNO at a tumor site can be induced, and peroxynitrite with higher activity is generated through combined action of active oxygen and NO, so that an efficient tumor cell killing effect is realized. Meanwhile, the released NO can effectively release a pancreatic cancer compact matrix, improve the tumor microenvironment and enhance the permeability of drugs and the infiltration capacity of immune cells. The invention also discloses an application of the nano-liposome system in pancreatic cancer treatment.

The present invention relates to a nano-drug delivery system that targets cancer cells in the hypoxic region of tumor microenvironment and a method for preparing same. The novel nano-drug delivery system according to the present invention can capture an extracellular matrix-degrading enzyme and encapsulate an anticancer drug by binding SO3-PEG-amine as a bifunctional ligand to manganese dioxide nanoparticles, thereby significantly improving drug permeability. In addition, through such high drug permeability, cancer cells in the hypoxic region of tumor microenvironment can be effectively killed.

To provide a drug delivery device (1000) having a drug-permeable component and a method for producing and using the same.SOLUTION: A drug delivery device includes a housing (1002) having a first wall structure (1012) and a second wall structure (1014) that are adjacent to each other and together form a tube (1010) defining a drug reservoir lumen (1004). The second wall structure or both the first wall structure and the second wall structure is or are permeable to water, and the first wall structure is impermeable to a drug while the second wall structure is permeable to the drug, such that the drug is releasable in vivo by diffusion through the second wall structure.SELECTED DRAWING: Figure 10A

The invention belongs to the field of biological tissue engineering, and discloses a culture method for promoting testicular tissue structural maturation in vitro and application. The method comprises the following steps: cutting a testicular tissue into blocky fragments with the volume of 0.5-1 mm; a gas-liquid interface culture mode is adopted, the lower portion of a pre-balanced 1.5% sepharose gel cubic support is soaked in a basic culture medium, the upper portion of the pre-balanced 1.5% sepharose gel cubic support makes contact with air, and then tissue blocks are placed on the support; the method comprises the following steps: adding a WNT5A pathway agonist Foxy-5 with the final concentration of 200-400 [mu] M into a basic culture medium containing alpha MEM, 10% KSR and 1% P / S, and continuously culturing for 11 days under the conditions of 34 DEG C and 5% COs. According to the method, a WNT5A signal channel is continuously activated by using Foxy-5 through gas-liquid interface culture, so that the polarity of a supporting cell can be effectively maintained, and the integrity of a blood-testis barrier (BTB) and the morphology regularization of a seminiferous tubule are promoted. According to the invention, a standardized and repeatable testicular tissue in-vitro maturation platform is established, and the method can be used for research in fields related to reproductive toxicity evaluation, drug permeability test and spermatogenesis microenvironment.