47 results about "Donor tissue" patented technology

A method and apparatus are disclosed for a high-throughput, large-scale molecular profiling of tissue specimens by retrieving a donor tissue specimen from an array of donor specimens, placing a sample of the donor specimen in an assigned location in a recipient array, providing substantial copies of the array, performing a different biological analysis of each copy, and storing the results of the analysis. The results may be compared to determine if there are correlations or discrepancies between the results of different biological analyses at each assigned location, and also compared to clinical information about the human patient from which the tissue was obtained. The results of similar analyses on corresponding sections of the array can be used as quality control devices, for example by subjecting the arrays to a single simultaneous investigative procedure. Uniform interpretation of the arrays can be obtained, and compared to interpretations of different observers.

A biomedical material for transplant to a subject is provided according to embodiments of the present invention which includes an isolated donor tissue enzyme-treated to reduce the amount of proteoglycans in the donor tissue compared to untreated tissue. Isolated cells are optionally added to the enzyme-treated donor tissue, including leukocytes, particularly monocytes; macrophages; platelets; cells derived from an intervertebral disc such as chondrocyte-like nucleus pulposus cells; fibrocytes; fibroblasts; mesenchymal stem cells; mesenchymal precursor cells; chondrocytes; or a combination of any of these. The isolated donor tissue is articular cartilage or an intervertebral disc tissue such as nucleus pulposus tissue and/or annulus fibrosis tissue enzyme-treated to remove proteoglycans normally present in these tissues. A biomedical material of the present invention is administered to a subject to treat a disorder or injury, such as a disorder or injury to connective tissue.

A tissue processing system includes a series of blades arranged in parallel to form a tissue processor. The blades may be adjusted to create a spaced between each blade in the range of 250-1000 microns. A slicer is included to remove a donor tissue to be processed by the processor. The processor is rotatable 90 degrees so as to create uniform micrografts of tissue for transplanting to a recipient site. An extractor is included to remove tissue particles from the processor after they have been cut into an appropriate size. A cutting block may be provided to ensure uniform thickness of cuts of the donor tissue.

A method of accurately fabricating a non-human donor corneal tissue for implantation into a recipient human cornea, the method comprising the steps of: removing corneal tissue from a donor with a femtosecond laser; placing the corneal tissue in a fixative solution for a selected time interval to cross-link the collagen fibrils in the tissue and prevent swelling of the corneal tissue; and shaping the tissue to provide a conical inlay of a selected shape and thickness having one or more radial extensions. The method is such that the corneal inlay may be attached to the peripheral corneal and/or the sclera. The method is such that the corneal inlay may be stored for a period of up to two years prior to attachment.

Disclosed is a method for expressing hepatitis C virus in an in vivo, animal model. Viable, hepatitis C virus-infected, human hepatocytes are transplanted into a liver parenchyma of a scid/scid mouse host. The scid/scid mouse host is then maintained in a viable state, for up to five days or greater, whereby viable, morphologically intact human hepatocytes persist in the donor tissue and hepatitis C virus is replicated in the persisting human hepatocytes.

A tissue engineering particle tissue and preparing process is characterized in that the tissue engineering particle tissue which is formed by cells to be compounded on micro-carriers comprises larger particle tissues which are formed by mutual connection of a plurality of particle tissues. The invention uses the tissue engineering technology to culture highly active tissue engineering particle tissues to implant in the body for repairing defected tissues and organs. The invention has a good cell growth state and higher cell activity, and can clinically solve the difficulties of less donor tissues and large repairing area, and the micro-carriers can finally be degraded, after the body is cured, no influences can be caused to the body. In the clinical treatment, the invention can be used as a transplant for repairing large area tissue defection and as filling material for repairing body depressed deformation, and can be transplanted to wound surfaces. The invention can also be used by injection, and is extensive in application range and convenient in utilization.

The inventors have discovered that hematologic disorders, e.g., both neoplastic (hematologic cancers) and non-neoplastic conditions, can be treated by the induction of mixed chimerism using myeloreductive, but not myeloablative, conditioning. Methods of the invention reduce GVHD, especially GVHD associated with mismatched allogeneic or xenogeneic donor tissue, yet provide, for example, significant graft-versus-leukemia (GVL) effect and the like.

A device transfers donor endothelial cells to the cornea of a recipient without injuring the cells or the cornea during the transfer process. The device transfers living endothelial cells from a donor to a recipient with minimal or no trauma. The device includes a handle, an irrigation tube lengthwise within the handle, a button upon the handle concentric with the irrigation tube, a tapered nose opposite the button, a forward tube extending outwardly from the nose, and a platform joined to the irrigation tube. The forward tube has an elliptical cross section and two beveled features. The platform has two wings that curl gently around donor tissue upon retracting the platform into the forward tube. The handle has a groove for a stem of a knob that surgeon presses and pulls to move the platform.

Provided is a method of treating an immune system disorder not involving T cell proliferation, comprising administering to the animal an immunotoxin comprising a mutant diphtheria toxin moiety linked to an antibody moiety which routes by the anti-CD3 pathway, or derivatives thereof under conditions such that the disorder is treated. Thus, the present method can treat graft-versus-host disease. Also provided is a method of inhibiting a rejection response by inducing immune tolerance in a recipient to a foreign mammalian donor tissue or cells, comprising the steps of: a) exposing the recipient to an immunotoxin so as to reduce the recipients's peripheral blood T-cell lymphocyte population by at least 80%, wherein the immunotoxin is anti-CD3 antibody linked to a diphtheria protein toxin, wherein the protein has a binding site mutation; and b) transplanting the donor cells into the recipient, whereby a rejection response by the recipient to the donor organ cell is inhibited, and the host is tolerized to the donor cell.

Decellularized matrix microspheres comprising a polymeric material and a donor tissue are provided. Also disclosed are structures containing a plurality of decellularized matrix microspheres incorporating a first polymer and a donor tissue; and a second polymer, wherein the decellularized matrix microspheres and the second polymer are in the form of a filament. Methods of treating a tissue injury employing the matrix microspheres and structures described as well as their methods of manufacture are also provided.

A system and method of resecting corneal tissue for transplantation is disclosed. In each of the recipient cornea and the donor cornea, an annular incision is made at a predetermined incision depth. A first sidecut incision is made in each cornea, running from the outer periphery of the annular incision to one of the anterior corneal surface or the posterior corneal surface. The first sidecut incision forms an acute angle with the annular incision. A second sidecut incision is also made in each cornea, running from the inner periphery of the annular incision to the other of the anterior corneal surface or the posterior corneal surface. The second sidecut incision forms an acute angle with the annular incision. The combination of the incisions in each cornea resects corneal tissue from the recipient cornea and donor tissue from the donor cornea. The donor tissue is grafted into the recipient cornea.

This present invention covers novel means, devices and instruments for production of a tissue array block that is further sectioned into duplicates of tissue arrays. An integral microscope is incorporated into the instrument for viewing and examining a stained reference slide and selecting donor tissue core region(s) from the reference slide. The reference slide is held in a reference slide station that is operatively linked and indexed with a station or platform holding a source donor tissue block, which is further indexed and precisely positioned with reference to the donor needle punch for punching the donor tissue core(s). A recipient block indexed to the donor block punch is placed under the donor punch station and donor tissue cores are delivered into pre-existing hole(s) by a stylet to construct the tissue array block. The instrument includes a donor punch station, optionally a second recipient punch station, with each operable independently or removable. The present invention also provides pre-loading needles with donor tissue cores for constructing tissue array blocks in pre-gridded and pre-punched recipient block. The tissue arrays produced from the tissue array blocks made are useful for testing such freshly-made and/or archival tissue specimens in both scientific and clinical research and applications.

A protector assembly for handling, storing and transporting donor tissue in a transfer pipette includes a distal cap and a proximal plug that are respectively engageable with opposite ends of the pipette. Structurally, the pipette includes a distally tapered fluid chamber which is in fluid communication with an extension tube. The distal cap is dimensioned for friction engagement with the fluid chamber of the pipette and it is formed with a plurality of vents to establish fluid communication through the vents with the fluid chamber. On the other hand, the proximal plug is dimensioned for a friction engagement with the extension tube to prevent leakage of solution from the fluid chamber of the pipette when the proximal plug is engaged with the pipette. In combination, an engagement of the protector assembly with a pipette holding a donor tissue will protect and preserve the donor tissue during handling and transport.

The invention relates to methods for promoting maturation of islet cells from pre-weaned mammals for the purpose of optimizing the islets for their use as donor tissue for xenotransplantation. The method of the invention removes the pancreas from donor animals and reduces the pancreas tissue to fragments that are greater than the size of an intact islet while retaining islets in their whole, insulin-producing condition. The method of the invention also serially cultures the digested tissue in novel maturation media that enhance the glucose responsiveness of the cultured islets, and selects islets that are sufficiently glucose-responsive for use in transplantation procedures.