48 results about "Tissue Processor" patented technology

A method of managing resources of a histological tissue processor, the tissue processor comprising at least one retort (12, 14) selectively connected for fluid communication to at least one of a plurality of reagent resources (26) by a valve mechanism (40), the method comprising the step of: nominating resources according to one of: group, where a group nomination corresponds to a resource's function; type, where a type nomination corresponds to one or more attributes of a resource within a group; station, where a station nomination corresponds to a point of supply of a resource.

A tissue processor for processing tissue samples for histological analysis. The processor comprises two retorts, wax baths, reagent containers, a pump and valve. The vale distributes the reagent from one container to either retort. Separate reagent lines connect the wax baths to the retorts. A method of infiltrating a sample containing a reagent such as a dehydrating reagent like an alcohol, where the infiltrating material is heated to a temperature at or above the boiling point of the reagent, to boil off the reagent when the tissue sample is contacted by the infiltrating material. The pressure in the retort may be reduced to lower the boiling point of the reagent.

An automatic tissue processor for processing tissue samples for histological analysis. The tissue processor in one configuration includes a retort chamber, a wax storage chamber holding multiple wax containers, a reagent storage chamber holding multiple reagent containers, and a fluid transporting system including a rotary valve operated by a Maltese Cross gear, for selectively connecting the retort chamber with a particular wax or reagent container to supply wax or reagents to the retort chamber. Differential heating of the wax chamber is accommodated by multiple heating elements, with indirect heating of the rotary valve and the fluid transporting system. A computerized central control system for automatic monitoring and control of such tissue processor is advantageously arranged to execute a reagent management program, which enables the central control system to automatically manage reagent usage and replacement.

A tissue processing system includes a series of blades arranged in parallel to form a tissue processor. The blades may be adjusted to create a spaced between each blade in the range of 250-1000 microns. A slicer is included to remove a donor tissue to be processed by the processor. The processor is rotatable 90 degrees so as to create uniform micrografts of tissue for transplanting to a recipient site. An extractor is included to remove tissue particles from the processor after they have been cut into an appropriate size. A cutting block may be provided to ensure uniform thickness of cuts of the donor tissue.

A tissue processor (1) is described, having a retort (2) for processing histological samples with different reagents, having multiple reagent reservoir vessels (3), and having a conduit system and a control device (5) for delivering reagents out of the reagent reservoir vessels (3) into the retort (2). A suction conduit (11) projects into each reagent reservoir vessel (3) through the vessel opening (7) for reagent withdrawal. A closure system (12) having an externally tapered and internally hollow closure plug (13) is provided for closing off the vessel opening (7). The suction conduit (11) is guided through a cavity (14) of the closure plug (13) for reagent withdrawal.

A rapid method of microwave-assisted formalin fixation of tissue that is described as: (a) a two-step process; (b) applicable to large (>5 mm thick) and small (<5 mm thick) tissues; (c) exposure of fresh tissue (i.e. a surgical biopsy) in a formalin solution to continuous microwave irradiation; (d) formalin circulated and cooled to maintain a constant temperature; (e) microwave-assisted formalin fixation performed at a temperature between 4° C. and 40° C.; (f) a temperature probe used to monitor fixative temperature in the cavity (g) after processing via microwave-assisted formalin fixation, the tissue can be processed by microwave methods or in an automatic tissue processor into paraffin for diagnostic evaluation. The two step process consists of a first low power microwave run followed by a second high power microwave run. An alternate embodiment of the present invention employing a one step MW oven process using other selected fixatives (glyoxal, glyoxal based and other aldehyde fixatives, alcohol, acetone, etc.)

A method for operating a tissue processor and a respective tissue processor for performing this method are described for the processing tissue samples. The tissue processor comprises at least one retort for receiving the tissue samples and at least one container for receiving a process medium. The process medium is transferred at least one of from the container into the retort and from the retort into the container. A value is automatically measured in the course of transferring the process medium, the value representing a characteristic property of the process medium. The process medium is identified based on the value.

A tissue processor for processing tissue samples for histological analysis. The processor comprises two retorts, wax baths, reagent containers, a pump and valve. The vale distributes the reagent from one container to either retort. Separate reagent lines connect the wax baths to the retorts. A method of infiltrating a sample containing a reagent such as a dehydrating reagent like an alcohol, where the infiltrating material is heated to a temperature at or above the boiling point of the reagent, to boil off the reagent when the tissue sample is contacted by the infiltrating material. The pressure in the retort may be reduced to lower the boiling point of the reagent.

A system includes an irreversible electroporation (IRE) pulse generator, a switching assembly, and a processor. The IRE pulse generator is configured to generate IRE pluses. The switching assembly is configured to deliver the IRE pulses to multiple electrodes that are disposed on an expandable distal end of a catheter that is placed in contact with tissue in an organ, for applying the IRE pulses to the tissue. The processor is configured to (a) receive one or more prespecified orientations along which electric fields in the tissue are to be generated by the IRE pulses, (b) select one or more pairs of the electrodes that would apply the IRE pulses at the prespecified orientations, and (c) connect the IRE pulse generator, using the switching assembly, to the selected pairs of the electrodes.

A tissue processing system includes a tissue processor for processing tissue using reagent fluids, a fluid container and a coupler providing bi-direction fluid communication between the tissue processor and the fluid container. The coupler includes first and second cylindrical rings separated by a wall and a fluid conduit disposed within the first and second cylindrical rings that pass through the wall, thereby providing fluid communication from the fluid container to the tissue processor. Fluid is returned from the tissue processor to the fluid container via at least one fluid return aperture in the wall that separates the first and second cylindrical rings.

The invention relates to a buffer system comprising maleic acid and maleic acid dipotassium salt, for use with formalin-containing fixative solutions. The buffer system is soluble / miscible in dehydration solutions including alcohols, ketones, and monomethyl ethers, thereby preventing formation of precipitates in fluid lines and valve systems of automated tissue processors.

Apparatus and methods for integrating tissue processors and embedding systems. An apparatus, of one aspect, includes a robot. The robot has a work envelope that encompasses a location having a tissue holder and an input of an embedding system. The tissue holder has at least one processed tissue. The robot is configured to transfer the tissue holder from the location to the input of the embedding system. A method, of one aspect, may include moving a robot to a location having a tissue holder. The tissue holder may have at least one processed tissue. The robot may engage with the tissue holder at the location. The robot may move the tissue holder from the location to an input to an embedding system. The robot may disengage from the tissue holder at the input to the embedding system. Other methods, apparatus, and systems are also disclosed.

A multispectral medical imaging device includes illumination devices arranged to illuminate target tissue. The illumination devices emit light of different near-infrared wavelength bands. The device further includes an objective lens, a near-infrared image sensor positioned to capture image frames reflected from the target tissue, and a visible-light image sensor positioned to capture image frames reflected from the target tissue. A processor is configured to modulate near-infrared light output of the plurality of illumination devices to illuminate the target tissue. The processor is further configured to determine reflectance intensities from the image frames captured by the near-infrared image sensor and to generate a dynamic tissue oxygen saturation map of the target tissue using the reflectance intensities. The device further includes an output device connected to the processor for displaying the dynamic tissue oxygen saturation map.

The invention provides a method for isolating and cultivating mouse cells, which includes the following steps: (1) sterilizing tissue processing equipment; (2) selecting male BALB / c mice aged 1 to 2 months, killing them by breaking their necks, and using surgical scissors. Separate the synovial layer and fibrous layer of the joint capsule, then remove the synovial layer tissue, put the tissue into pre-cooled sterile PBS solution containing double antibody and wash it several times to remove blood stains and fatty tissue; (3) Cut the tissue into pieces, and use Digest with preheated type II collagenase and trypsin respectively; (4) Stop digestion, centrifuge, and discard the supernatant; (5) Inoculate into the coated culture flask by manual adhesion method; (6) After incubation for 1 to 2 hours, Cultivate in a 37°C, 5% CO2 environment; (7) Cell morphology observation; (8) Cell subculture; (9) Cell identification. The method for isolating and culturing mouse synovial cells provided by the invention is simple to operate, has a large number of cells obtained, and has a high survival rate. It is an ideal primary method for isolating and cultivating mouse synovial cells and provides reliable results for experiments. Cell resources.

A sensor module (38, 40, 42) for a tissue processor (20) has a thermal sensor (66) for measuring a temperature of a container (30, 32, 34) of the tissue processor (20), and further has a position sensor for detecting a working position of the container (30, 32, 34).

The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.

A tissue processor (1) is described, having a retort (2) for processing histological samples with different reagents, having multiple reagent reservoir vessels (3), and having a conduit system and a control device (5) for delivering reagents out of the reagent reservoir vessels (3) into the retort (2). A suction conduit (11) projects into each reagent reservoir vessel (3) through the vessel opening (7) for reagent withdrawal. A closure system (12) having an externally tapered and internally hollow closure plug (13) is provided for closing off the vessel opening (7). The suction conduit (11) is guided through a cavity (14) of the closure plug (13) for reagent withdrawal.

A method of managing resources of a histological tissue processor, the tissue processor comprising at least one retort (12, 14) selectively connected for fluid communication to at least one of a plurality of reagent resources (26) by a valve mechanism (40), the method comprising the step of: nominating resources according to one of: group, where a group nomination corresponds to a resource's function; type, where a type nomination corresponds to one or more attributes of a resource within a group; station, where a station nomination corresponds to a point of supply of a resource.

The invention provides a human primary colon cancer cell separation and culture method. The method comprises the following steps of (1) cutting a fresh colon cancer tissue under an aseptic condition,sealing at 4 DEG C and then carrying back to a lab; (2) disinfecting a tissue processing device; (3) using a pair of operating scissors for separating the colon cancer tissue, placing the tissue intoa precooled sterile PBS liquid containing double antibodies, and washing multiple times to remove blood vessels, fat and necrotic tissues; (4) cutting up the tissues, and using preheated pancreatin and IV type collagenase for digesting respectively; (5) terminating digestion, centrifuging, and removing a supernatant; (6) using trypan-blue for dyeing a cell filter liquor to detect cell activity; (7) after carrying out cell counting, inoculating into a coated culture flask; (8) culturing at 37 DEG C in an environment with 5 percent of CO2; (9) purifying the cells; (10) subculturing the tissues;(11) observing cell morphology; (12) determining cell survival rate; (13) carrying out immunological identification. The invention provides the human primary colon cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human primary colon cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.

A tissue processor used for processing tissue sample for histopathology analysis, comprising two retorts, a paraffin tank, a reactant vessel, a bump and a valve from a vessel to any retort to distribute the reactant; a divided reactant tube is connected to the paraffin tank to the retort; a method of permeating a sample comprises a reactant like alcohol; wherein the temperature of the permeation material is heated to equal to or higher than the boiling point of the reactant, so that the reactant can be removed through boiling when using the permeation material in contact with the tissue sample, which can reduce the pressure in the retort so as to reduce the boiling point of the reactant.

The invention provides a method for isolating and culturing rat Schwann cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting 2-3 week old male SD rats, killing them by neck dislocation, and using ophthalmic scissors Cut off the hair on the legs of rats, cut off the sciatic nerve, put the tissue into pre-cooled sterile PBS solution containing double antibodies and wash several times to remove blood stains and adventitia; (3) Mince the tissue, and use preheated type II collagen (4) Terminate the digestion, centrifuge, discard the supernatant, add culture medium and resuspend; (5) Inoculate the tissue block into a 6-well culture plate for culture by adherent method; (6) Incubate for 1-2 hours, Placed in a 37°C, 5% CO2 environment for culture; (7) Cell subculture and purification; (8) Cell morphology observation and identification. The method for separating and culturing rat Schwann cells provided by the present invention is simple and easy to operate, stable and efficient, and the obtained Schwann cells have high vigor, which can be used to establish model cells for in vitro experiments and meet the requirements of rat Schwann cell experiments .

The invention provides a human primary gastric cancer cell separation and culture method. The method comprises the following steps of (1) disinfecting a tissue processing device; (2) using a pair of operating scissors for separating a gastric cancer tissue, placing the tissue into a precooled sterile PBS liquid containing double antibodies, and washing for multiple times to remove bloodstains andnecrotic tissues; (3) cutting up the tissues, and using preheated IV type collagenase and trypsin for digesting respectively; (4) terminating digestion, centrifuging, and removing a supernatant; (5) after carrying out cell counting, inoculating into a coated culture flask; (6) culturing at 37 DEG C in an environment with 5 percent of CO2; (7) purifying the cells; (8) subculturing the tissues; (9)observing cell morphology; (10) determining cell survival rate; (11) carrying out immunological identification. The invention provides the human primary gastric cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human gastric cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.

A method of operating a tissue processor for processing tissue samples is provided. The tissue processor includes at least one retort for receiving tissue samples, at least one container for storing areagent, and at least one sensor arranged for fluid communication with one or both of the at least one container and the at least one retort for measuring a measured purity level of a reagent. The method includes the steps of conducting reagent from the at least one container or the at least one retort to the at least one sensor, automatically measuring, by means of the at least one sensor, a measured purity level of the reagent, checking whether the measured purity level meets a predetermined purity level of the reagent associated with the at least one container, and automatically determining, based on a result of checking, whether the reagent is suitable for processing tissue samples in the tissue processor. A tissue processor for processing tissue samples is also provided. A containeris also provided for storing tissue samples for processing in a tissue processor.

A tissue processor for treating tissue samples comprises a process chamber (10) in which the tissue samples can be treated with at least one liquid. The process chamber (10) is formed as a vessel of a magnetic stirrer. In the process chamber, a stirring body (16) for stirring the liquid is arranged, wherein the stirring body (16) comprises at least one magnet (40a, 40b) with the aid of which the stirring body (16) can be set in rotation by a drive unit (20) of the magnetic stirrer, and at least one vane (28a to 28f) by which the liquid to be stirred is stirred when the stirring body (16) rotates. According to a first aspect of the invention, the stirring body (16) has at least three vanes (28a to 28f), according to a second aspect of the invention, the stirring body (16) has at least one further magnet (40b). The north pole (46) of the one magnet (40a) and the south pole (50) of the further magnet (40b) face the drive unit (20).

The invention provides a single cavity tissue histology tissue-processor. A system and a method for processing tissue specimens, comprising a cavity for processing said tissue specimens, and a fluid transfer system to transfer at least one of a plurality of fluids from a plurality of storage containers to the inside and out of said cavity. The cavity is connected to at least one of a microwave generating device for applying microwave radiation to an inside of the cavity, and a thermal energy generating device for generating a positive thermal energy gradient inside the cavity.

The embodiment of the invention discloses a device, a method and a tissue treatment machine for prompting the state of a solvent in a solvent bottle in a tissue processor. The device comprises a detector, a controller and a prompter. The detector is used for detecting the state of the solvent. The controller is used for generating a control signal according to the state. The prompter is used for generating a signal for prompting the state of the solvent according to the control signal. According to the embodiment of the invention, an operator can be visually reminded of the state of the solvent through the signal for prompting the state of the solvent, so that the operator can accurately replace the solvent in time, and solvent replacement errors are avoided.