44 results about "Double antibody" patented technology

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention discloses an efficient purification process of a bispecific antibody and a special chromatography system thereof. The efficient purification process of the bispecific antibody comprises the following steps: pretreatment: performing 0.22 mu m microfiltration on a cell culture supernatant to remove cell debris, and performing deep filtration to reduce turbidity to be less than or equal to 1NTU so as to reduce subsequent chromatography medium pollution; carrying out improved Protein A affinity chromatography, namely carrying out affinity chromatography by adopting an agarose medium modified by a recombinant Protein A ligand, taking 20mM Tris-HCl as a loading buffer solution, and taking the pH value of the loading buffer solution as 7.4, taking 100mM citric acid as an elution buffer solution, and taking the pH value of the elution buffer solution as 3.2-3.5; performing mixed mode chromatography: adopting a composite mode medium containing a benzamidine functional group, taking 50mM sodium phosphate as a buffer solution, adjusting the pH value to 6.0-6.5, adjusting the NaCl concentration to 0.1-0.3 M, and specifically removing a double-antibody polymer and a mismatched isomer by utilizing a hydrophobic effect and an ion exchange synergistic effect; and performing nanoscale filtration and terminal treatment: removing viruses by adopting a virus filtration membrane with the aperture of 30nm, and performing 0.22 mu m sterile filtration to obtain a final product. According to the process, the purification efficiency, the yield and the product purity are remarkably improved, and the process is suitable for efficient purification of the bispecific antibody.

The invention provides a double-antibody directionally-coupled FET (Field Effect Transistor) biosensor and a preparation method thereof, and belongs to the technical field of biosensors. The FET biosensor adopts a reference electrode to provide grid voltage, a buffer solution is a conductive electrolyte, the FET biosensor comprises a field effect transistor, a capture antibody and a detection antibody, the field effect transistor is composed of a source electrode, a drain electrode and a channel between the source electrode and the drain electrode, the bottom layer of the channel is an indium oxide thin film layer, the indium oxide thin film layer is modified with amino, and the detection antibody is a detection antibody. An amino-modified channel is obtained; carrying out passivation treatment on the source electrode and the drain electrode by adopting a mercaptan passivator; and cross-linking and fixing the capture antibody and the detection antibody on the channel modified with the amino group through a cross-linking fixing agent to obtain the FET biosensor. The two antibodies are combined according to different epitopes of antigens to form a sandwich structure, and double recognition of the antibodies on target antigens is utilized to enhance antigen capture efficiency and signal enrichment, so that a double-site recognition mode is formed, and low-concentration antigen detection is realized.

The invention relates to the field of biology, and discloses a double-antibody connection probe for detecting lactylated PGK1, a preparation method and application, the double-antibody connection probe comprises an antibody A, an antibody B and complementary oligonucleotides respectively coupled with the antibody A and the antibody B; the antibody A is a polypeptide which is subjected to K139-lactic acid modification and is used for recognizing a K139la site of the PGK1; the antibody B is a polypeptide which is used for identifying more than or equal to 50 amino acids away from the K139 site in the PGK1 and has the length of 12-15 amino acids; the double-antibody connection probe obtained by the invention realizes the ultra-sensitive detection of the targeted protein.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

The invention discloses an efficient mouse small intestine epithelial cell isolated culture method. The method comprises the steps of fetal mouse small intestine tissue obtaining, flushing digestion, sediment obtaining through centrifugal separation, density gradient centrifugal purification, differential adhesion method purification, inoculated culture and culture medium replacement for further culture. During culture, a first culture medium and a second culture medium based on William's E basic culture media are sequentially used, and the first culture medium comprises fetal calf serum, EGF, L-glutamine, mannitol, methylprednisone, bovine insulin and double antibodies; the second culture medium is prepared from fetal calf serum, vitamin C, L-glutamine, resveratrol, sodium pyruvate, bovine insulin and double antibodies. The rapid adherent proliferation and long-term stable culture of the mouse small intestine epithelial cells on the 2D surface are realized through mutual cooperation of the two culture media, so that the separation and in-vitro culture method of the mouse small intestine epithelial cells, which is simple, easy to operate, stable and efficient, is established, and convenience is provided for metabolic research of various in-vitro drugs and inflammatory factors.

The invention belongs to the technical field of microbial viruses, and particularly relates to a culture method for improving the virus titer of duck flavivirus, which comprises the following steps: step 1, preparing a culture solution, accurately weighing RPMI1640 dry powder, dissolving the RPMI1640 dry powder in three-distilled water, then adding a proper amount of fetal calf serum and double antibodies, filtering and sterilizing, then sub-packaging into sterile culture bottles, and preparing the culture solution for later use; storing at a proper temperature for later use; 2, preparing cells, selecting suitable cells, inoculating the cells into a culture flask, and carrying out single-layer culture by using an RPMI1640 culture solution to ensure that the cells are in a good growth state before the cells are inoculated with viruses, The method has the beneficial effects that by selecting a suitable host cell line, the cells are ensured to be in a healthy state, the performance reduction caused by high algebraic culture is avoided, and the cell quality is improved. In addition, the cell culture medium suitable for virus production is selected, and the culture medium with high transfection efficiency and cell maintaining capacity after transfection can remarkably improve the virus titer.

The application provides a kind of bispecific antibody and its preparation and application, the bispecific antibody includes two chains: from N end to C end in order: VH1 (or VL1), L1, VH2, CH1, the first polypeptide chain of Fc, and from N end to C end in order: VL1 (or VH1), L2, VL2, CL, second polypeptide chain.It is realized tumor selective accumulation by being located in the N end of double antibody structure of the binding domain targeted to tumor associated antigen (TAA), and its shield is located in the inside immune checkpoint antibody fragment to the binding activity of immune checkpoint to reduce systemic activity.

The present application provides an amniotic stromal cell culture system, a cell culture method and an application, which belong to the field of tissue repair technology. The amniotic stromal cell culture system includes DMEM / F12 culture medium, fetal bovine serum, double antibody and uridine. Fetal bovine serum can provide the necessary nutrients for the growth of amniotic stromal cells, hormones to maintain cell exponential growth, provide binding proteins, etc., and promote the proliferation of amniotic stromal cells. Uridine has anti-inflammatory and antioxidant effects, can reduce the damage of reactive oxygen species to cells, and also promotes cell proliferation and activity by activating signal pathways such as PI3K / Akt, so that the cultured cells can accelerate wound healing and are beneficial to tissue repair. The amniotic stromal cells cultured in this amniotic stromal cell culture system can significantly improve the repair effect of intravenously infused amniotic stromal cells on intestinal damage, and can be used to prepare drugs for treating intestinal damage.

The invention provides a preservation and culture method suitable for in-vitro tissue of a cell line, which comprises the following steps: firstly, cleaning the in-vitro tissue by using PBS (Phosphate Buffer Solution) containing double antibodies, scraping foreign matters on the surface, and then respectively cleaning twice by using 75% alcohol and PBS containing double antibodies to obtain a pretreated sample; then, the pretreated sample is loaded into a sterile sample bag and is vacuumized, and the sterile sample bag is placed in a precooling biological safety transport box, so that long-time and long-distance transport is realized, and cell activity is maintained; and finally, carrying out somatic cell separation and culture on the pretreated sample to obtain primary cells. According to the method, through combination of vacuum treatment and preservation at 4 DEG C, oxidative damage and autolysis of tissues are effectively delayed, and the risks that genetic resources of precious somatic cells are polluted and line establishment fails under difficult conditions such as remote areas are remarkably reduced; through verification, the line can still be successfully established after the tissue is preserved for 14 days, and cells can be stably passaged for 20 generations in vitro in cooperation with an optimized culture medium.

The invention provides a fully humanized antibody or a single-chain antibody or a fragment thereof targeting BAFF-R, the fully humanized antibody or the single-chain antibody or the fragment thereof is combined with BAFF-R with high affinity and high specificity, and compared with a heterologous antibody, the fully humanized antibody or the single-chain antibody or the fragment thereof has lower immunogenicity and has good application potential in development of antibody drugs (including monoclonal antibodies, double antibodies, antibody coupling drugs (ADC) and the like) and cell therapy drugs (including CAR-T, CAR-NK and the like).

The present application belongs to the technical field of tumor detection and cell separation, and particularly relates to a kit for separating and recovering after immunocapture of circulating tumor cells, a preparation method and application. The kit comprises an immunocapture module, a dissociation and recovery module, a cell protection module and elution and supplement factor solutions. The immunocapture module adopts magnetic microspheres modified by double antibody fusion proteins, can recognize multiple markers such as EpCAM and Vimentin, and realizes efficient capture of heterogeneous CTCs. The dissociation and recovery module uses a low-temperature buffer system and a cleavable cross-linking agent to release cells gently. The cell protection module maintains the integrity and activity of the cell membrane during the separation process. The method of the present application can ensure the capture efficiency while obtaining high-activity CTCs, and is suitable for single-cell sequencing, drug sensitivity analysis, organoid culture and patient-derived cell line construction and other preclinical research.

The invention belongs to the technical field of agricultural product detection, and particularly relates to a primer group for detecting 14 transformants of corn and application of the primer group. According to the invention, a primer group of which the nucleotide sequences are shown as SEQ ID NO. 1-SEQ ID NO. 75 is provided, and the primer group can realize specific detection on 14 corn transformants such as 4114, MIR162, DBN9501, MON88017, GA21, MON89034, NK603, double antibody 12-5, 591 22, BT11, ND207, DBN9936, TC1507 and MIR604, so that the specificity is good, and the sensitivity is high. Furthermore, the invention also provides a kit and a method for detecting the corn transformant, an LAMP + OSD fluorescent probe detection system is constructed by using the upper primer and an OSD probe with a nucleotide sequence as shown in SEQ ID NO.76-SEQ ID NO.103, so that the specific transgenic transformant in corn and products thereof can be accurately, specifically and reliably detected and identified, and the corn transformant can be used for detecting the corn transformant. The method is especially used for detecting gene edited corn. Therefore, the technical scheme of the invention has the advantages of low detection cost, quick and accurate result and strong practicability.

This invention, entitled "Method, Hybridoma Cells and Their Derivatives, and Applications for Detecting β-1,4-N-acetylgalactosamine transferase (GalNAcT)," belongs to the field of ELISA detection technology. The technical problem to be solved is to improve the limit of detection. The key technical solution is the use of a double-antibody immunosandwich assay, wherein the double antibodies in the double-antibody immunosandwich assay include antibody 1 and / or antibody 2; antibody 1 is secreted by a hybridoma cell line with accession number GDMCC No. 66735, and antibody 2 is secreted by a hybridoma cell line with accession number GDMCC No. 66736.

The invention relates to the technical field of biological detection, and discloses a saliva exosome detection kit for non-invasive auxiliary screening of Alzheimer's disease and a preparation method of the saliva exosome detection kit. The specific exosome enrichment system composed of the anti-L1CAM / CD63 double antibody coated capture plate and the anti-MUC1 / KRT19Fab fragment blocking agent is constructed for the first time, about 85% of oral cavity source exosomes can be effectively removed, and the capture purity of neuron source exosomes is remarkably improved; the special saliva preserving fluid containing DTT and a specific enzyme inhibitor is developed, the technical bottlenecks that saliva RNA is easy to degrade and a sample is uneven in viscosity are overcome, and the room temperature stability is prolonged to 8 hours; an RT-qPCR detection system taking saliva exosome miRNA-135a as a core and taking internal reference miRNA-16-5p standardization is established, and clinical sample verification shows that the kit can effectively distinguish the Alzheimer's disease patient from a healthy control, shows excellent inter-group distinguishing ability, and provides a stable, efficient, reliable and complete technical scheme for solving the problem of AD non-invasive early screening.

The invention provides a double-antibody modified vesicle STING agonist as well as a preparation method and application thereof, and relates to the technical field of polymer nano-drugs. The double-antibody modified vesicle STING agonist comprises a vesicle, an STING agonist and a double-antibody, the vesicle is formed by cross-linking and assembling a functionalized amphiphilic block polymer and an amphiphilic block polymer, an inner cavity of the vesicle is electronegative, the STING agonist is loaded in the inner cavity of the vesicle through electrostatic interaction, and the double-antibody modified vesicle STING agonist is loaded in the inner cavity of the vesicle. The double antibody comprises an anti-HER2 monoclonal antibody and an anti-PD1 monoclonal antibody which are grafted on the surface of the vesicle. The double-antibody modified vesicle STING agonist has good biological safety and can enhance the killing effect of T cells on tumor cells, so that the tumor cells are killed.

The invention discloses a GV-stage granulosa cell extraction method, which is characterized in that the number of granulosa cells is diluted to 4-6 * 10 < 5 > / L in the extraction process, the extracted cells can be utilized to the maximum extent, PSB is used for flushing in the extraction process, and high-glucose DMEM containing 0.8-1.2% of double antibodies and 8-12% of FBS is used, so that adherent culture of the granulosa cells is neater and cleaner, and the GV-stage granulosa cells are obtained. And adherence and growth of GV-stage granular cells are facilitated.

The present application relates to a kind of double antibody drug conjugate targeted to PD-L1 and VEGF and its preparation method and application.The general formula of the double antibody drug conjugate is Ab-(L-D) x;In general formula Ab, it is the double antibody targeted to PD-L1 and VEGF, includes PD-L1 antigen binding portion and VEGF antigen binding portion;In general formula L-D, it is connection unit-drug compound, L is connection unit, D is drug molecule, x is selected from the integer in 1~8.The double antibody drug conjugate includes PD-L1 antigen binding portion, VEGF antigen binding portion and drug molecule, and the synergistic efficacy between anti-angiogenesis, PD-L1 immune target and antibody conjugate is realized by reasonable structure design.

The invention belongs to the field of biological medicine, and particularly relates to a bispecific binding protein structure of a T cell connector, nucleic acid, a carrier, a cell and a preparation method and application of the bispecific binding protein structure. The invention provides a bispecific binding protein of a T cell connector, the bispecific binding protein comprises a TCE double antibody structure of an anti-CD3 antibody sequence, a Fab / Fab-Fab Fc structure or a Fab / Fab-scFv Fc structure is used to construct a bispecific antibody of a 2 + 1 valence tumor-associated antigen and a CD3 protein, and the bispecific binding protein has good tumor cell killing activity after being combined with different TAAs.

The present application relates to anti-SMPDL3A monoclonal antibodies Sm-1 and Sm-4, and a diagnostic kit for early colorectal cancer prepared therefrom. The present application successfully prepared and screened a plurality of high-affinity anti-SMPDL3A monoclonal antibodies, which laid a material foundation for subsequent establishment of a high-sensitivity immunodetection method. Through systematic double antibody pairing screening, the present application found that Sm-1 and Sm-4 can recognize different epitopes of SMPDL3A, and the spatial distance between them is far and they do not interfere with each other, so as to form a stable "sandwich" sandwich structure; and the remaining antibody combinations have poor pairing effect due to adjacent epitopes or spatial steric hindrance. The preferred combination significantly improves the signal-to-noise ratio and stability of the double antibody sandwich ELISA. The detection kit provided by the present application can be directly applied to serum samples, is simple to operate, and has controllable cost, thereby providing a new serological marker and effective technical means for early non-invasive diagnosis of colorectal cancer, and being suitable for large-scale population screening, and having a good clinical application prospect.

The invention discloses a composition for constructing an IOPN organ model, a model construction method and application. The composition for constructing the IOPN organoid model comprises an organoid model culture medium, and the organoid model culture medium comprises a penicillin-streptomycin double antibody solution, GlutaMAX, a serum-free supplement, Rspo-1, a Wnt activator, a growth factor, an HEPES buffer solution, N-acetyl-L-cysteine, a gastrin hormone, nicotinamide, forskolin, an ALK inhibitor, a ROCK inhibitor and the balance of a basic culture medium. The IOPN type organ model cultured by the composition for constructing the IOPN type organ model can maintain the original characteristics of tumors, provides a reliable biological model for pathological research and drug research and development of pancreatic tumors, and is convenient to operate and low in cost.

The present disclosure provides methods for treating cancer in a patient comprising administering a combination therapy comprising a double antibody drug conjugate. In some embodiments, the combination therapy comprises: (i) a therapeutically effective amount of a Trop-2 antibody-drug conjugate (Trop-2 ADC); (ii) a therapeutically effective amount of a Nectin-4 antibody-drug conjugate (Nectin-4 ADC); and optionally, (iii) a therapeutically effective amount of an anti-PD (L)-1 antibody. In some embodiments, the cancer is urothelial cancer.

The application provides a method for genetic reactivation of individual Procyprinus palpebrosus, comprising the following steps: S1, taking the gonadal tissue of Procyprinus palpebrosus, washing, cutting, adding L-15 culture medium containing double antibodies and DPBS buffer solution to centrifuge the gonadal tissue; S2, water-bathing the gonadal tissue to obtain recovered gonadal tissue; S3, digesting the recovered gonadal tissue, filtering, centrifuging and purifying to obtain germ stem cells; S4, injecting morpholino to obtain rare gobi-ocypris rarus with deleted primordial germ cells; S5, transplanting the germ stem cells into the body of the rare gobi-ocypris rarus juvenile to culture, and obtaining the gametes of Procyprinus palpebrosus; and S6, fertilizing the generated gametes of Procyprinus palpebrosus to obtain the individual Procyprinus palpebrosus. The application successfully obtains the sperm of Procyprinus palpebrosus by using the cryopreserved gonadal tissue and the technology of parthenogenesis, and provides a feasible way for the individual genetic reactivation of rare and endangered fish species.

The invention discloses a method for determining the binding activity of epidermal growth factors in sodium hyaluronate composite gel. The method comprises the following steps: S1, weighing HE and carrying out enzymolysis to obtain a pre-diluent of a test sample; s2, weighing a human epidermal growth factor activity determination standard substance and redissolving to obtain a linear standard substance solution; s3, diluting the pre-diluent to enable the pre-diluent to be within a detection linear range to obtain a test solution; s4, incubating the standard solution and the test solution; s5, adding a color developing solution after incubation, incubating in a dark place, and adding a stop solution after color development; s6, putting into a microplate reader, measuring absorbance, and recording a result; and S7, drawing a standard curve and analyzing the sample concentration according to the result, and calculating the percentage of the sample concentration in the theoretical value. According to the invention, sodium hyaluronate enzyme is added, so that sodium hyaluronate in the gel can be subjected to enzymolysis, the constraint of the sodium hyaluronate hydrogel with a net structure on EGF in a test sample is relieved, the influence of a double-antibody one-step sandwich method ELISA test on the detection of the EGF content in HE is reduced, and the accuracy of a detection result is improved.

The invention provides an anti-CD3 monoclonal antibody, a bispecific antibody and application of the anti-CD3 monoclonal antibody and the bispecific antibody, and particularly provides an antibody or an antigen binding fragment which comprises at least one of the following CDRs: a heavy chain variable region CDR in SEQ ID NO: 1-3 or a conservatively modified form thereof; sEQ ID NO: 4-6 or a light chain variable region CDR in a conservative modification form thereof. The antibody or the antigen binding fragment has different affinity with CD3 protein at different pH values, has weak binding capacity in animal tissues or peripheral blood with normal pH values, has low peripheral drug concentration, reduces off-target toxicity risk, has strong affinity with CD3 at tumor tissues or around the tumor tissues, is beneficial to distribution to local tumor, increases local drug concentration, and reduces the risk of off-target toxicity of the antibody or the antigen binding fragment in the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues or around the tumor tissues. The double antibodies prepared from the polypeptide can promote the combination of T cells and tumor cells, are high in safety and specificity, and can effectively treat tumors, autoimmune diseases, inflammatory diseases and / or transplant rejection.

The invention relates to a colloidal gold kit for diagnosis of early pregnancy of cattle and application of an antibody pair of the colloidal gold kit. The antibody pair consists of a first monoclonal antibody and a second monoclonal antibody, wherein the amino acid sequences of CDR1, CDR2 and CDR3 on a light chain variable region of the first monoclonal antibody are respectively shown as SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7, and the amino acid sequences of CDR1, CDR2 and CDR3 on a heavy chain variable region of the first monoclonal antibody are respectively shown as SEQ ID NO: 12, SEQ ID NO: 13 and SEQ ID NO: 14; amino acid sequences of CDR1, CDR2 and CDR3 on a light chain variable region of the second monoclonal antibody are respectively shown as SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21, and amino acid sequences of CDR1, CDR2 and CDR3 on a heavy chain variable region of the second monoclonal antibody are respectively shown as SEQ ID NO: 26, SEQ ID NO: 27 and SEQ ID NO: 28. The antibody pair with high specificity and high affinity is provided, the pairing problem of traditional double-antibody detection is solved, the detection sensitivity is remarkably improved, and early pregnancy diagnosis can be carried out 24 days after hybridization.

The invention discloses a functional exosome as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The exosome highly expresses miR-124-3p and miR-10a-5p, and the miR-124-3p can inhibit degradation of an extracellular matrix and promote remodeling of the extracellular matrix by inhibiting signal molecules such as ROCK1 and the like; and the latter can inhibit a proinflammatory factor receptor (IL6R) in a targeted manner so as to inhibit local inflammation. According to the dual synergistic effect of matrix remodeling and anti-inflammatory regulation, accurate intervention is carried out on core pathological links (matrix degradation and inflammation) of intervertebral disc degeneration, and the treatment effect which is difficult to achieve by a single therapy is achieved. According to the exosome, Syntenin-1 and IL-1RA are taken as dual molecular targets for screening functional exosome subgroups, and the specific exosome subgroups with dual functional potentials of'tissue repair 'and'immune regulation' are accurately screened from a complex exosome group derived from fibroblasts through a double-antibody immunoaffinity method.

The present invention relates to the field of cancer immunotherapy. In particular, the present application relates, in some aspects, to binding molecules, such as complexes capable of specifically binding a major histocompatibility complex (MHC) molecule and an AFP peptide. The present invention relates to T cell receptors (TCRs), including antigen binding domains thereof, that bind to or recognize such peptide epitopes. The present application further relates to engineered cells comprising such binding molecules, such as TCR double antibody proteins or TCRs, and the like, and their use in cancer immunotherapy.

The invention relates to the technical field of biology, in particular to a bifunctional antibody for promoting wound healing reaction and a pharmaceutical composition thereof, at least comprising: a first protein functional region targeting CD4, the first protein functional region being an anti-CD4 antibody or an antigen binding fragment thereof; a second protein functional region, the second protein functional region targeting TGF [beta] 1 / [beta] 2 (especially not targeting TGF [beta] 3), the second protein functional region being an anti-TGF [beta] 1 / [beta] 2 (especially not anti-TGF [beta] 3) antibody or an antigen binding fragment thereof; the double antibody can be safely combined with CD4, but the function of the CD4 is not influenced; by combining with CD4, the double antibody can specifically inhibit a TGF-beta1 signal of CD4 + T cells, so that the inhibition effect of TGF-beta1 on the CD4 + T cells is relieved, and the wound healing reaction mediated by the TGF-beta1 is released; meanwhile, the double antibodies can inhibit TGF-beta2 so as to inhibit the fibrosis promotion capability of the TGF-beta2, and have no inhibition capability on TGF-beta3, so that the wound healing promotion capability of the double antibodies is reserved, and the double antibodies can be used for reducing the scar area after wound healing.