169 results about "Glutathione S-transferase" patented technology

The present invention provides new and improved assay for detection of genomic methylated CpG islands. This new method is termed the methylated-CpG island recovery assay (MIRA). In accordance with one embodiment, MIRA comprises the steps of: (a) incubating genomic DNA fragments with a methylated CpG island binding protein in the presence of a binding partner for the binding protein to produce bound DNA containing methylated CpG islands, (b) isolating the bound DNA, and (c) detecting CpG island methylation by gene-specific amplification reactions. In accordance with a preferred embodiment, MIRA comprises the steps of: (a) incubating sonicated genomic DNA with a matrix containing a fusion protein of glutathione S-transferase (GST) and MBD2b (GST-MBD2b) in the presence of MBD3L1 to produce bound DNA containing methylated CpG islands, (b) eluting bound DNA from the matrix, and (c) detecting CpG island methylation by gene-specific amplification reactions.

A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3′-phosphoadenosine 5′-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.1-protein, AT1 G53885-protein, AT2G35300-protein, AT3G04620-protein, AT4G01870-protein, AT5G42380-protein, AT5G47440-protein, CDS5394-protein, CDS5401_TRUNCATED-protein, cold response protein, cullin, Cytochrome P450, delta-8 sphingolipid desaturase, galactinol synthase, glutathione-S-transferase, GTPase, haspin-related protein, heat shock protein, heat shock transcription factor, histone H2B, jasmonate-zim-domain protein, mitochondrial asparaginyl-tRNA synthetase, Oligosaccharyltransferase, OS02G44730-protein, Oxygen-evolving enhancer protein, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase family protein, plastid lipid-associated protein, Polypyrimidine tract binding protein, PRLI-interacting factor, protein kinase, protein kinase family protein, rubisco subunit binding-protein beta subunit, serine acetyltransferase, serine hydroxymethyltransferase, small heat shock protein, S-ribosylhomocysteinase, sugar transporter, Thioredoxin H-type, ubiquitin-conjugating enzyme, ubiquitin-protein ligase, universal stress protein family protein, and Vacuolar protein.

The invention discloses a fluorescent probe for detecting the content of glutathione (GSH) in blood, and a synthesis method and an application thereof. The synthesis steps of the probe are simple, and the light stability is good. In the detection process, the fluorescent probe firstly as a substrate is combined with glutathione S-transferase (GST), and undergoes a reaction with another specific substrate GSH of the GST to produce fluorescence, the fluorescence is enhanced by about 35 times, and the maximum emission wavelength is at the near infrared region (beyond 700 nm). Compared with conventional GSH fluorescent probes, the probe can specifically detect the content of the GSH in a complex system, and has the advantages of wide dynamic response window, high efficiency and the like. The probe realizes the specific and high-efficiency identification on the GSH in the complex environment, and has extremely important application value in the biological and medical fields.

The invention belongs to the technical field of biological monitoring environment, and specifically relates to a shellfish monitoring method for ocean oil spill pollution. The shellfish monitoring method comprises the steps of utilizing typical bivalve mollusk such as short-necked clam and chlamys farreri in China to determine the shellfish visceral mass autioxidant enzyme activities including superoxide dismutase, glutathione glutathione S-transferase, peroxidase, glutathione peroxidase and catalase activity, integrating the enzyme activities so as to form an integration biomarker responding index (IBR), establishing a dose-effect relationship between the IBNNR and petroleum concentration, adopting a one-dimensional variance components method (ANOVA) to carry out notable difference analysis, determining the forcing degree of petroleum pollution on organisms, and finally judging pollution level of the ocean oil spill.

Polyacrylamide-based methods of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics are described. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of glutathione S-transferase-green fluorescent protein (GST-GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng / mm2) was detectable by direct measurement of green fluorescent protein fluorescence and this lower detection limit was reduced to 0.080 ng / mm2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src which is encoded by the Rous Sarcoma virus. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

The invention discloses a rice cadmium-tolerant gene OsGSTU37 and application thereof.Sequences of the rice cadmium-tolerant gene OsGSTU37 are shown as SEQ ID NO:1 in sequence tables, and amino acid sequences of glutathione S-transferase (GSTs) of an encoding protein of the rice cadmium-tolerant gene OsGSTU37 are shown as SEQ ID NO:2 in the sequence tables.As proved by ectopic expression of yeast, the rice cadmium-tolerant gene OsGSTU37 has cadmium toxicity tolerant characteristics.The rice cadmium-tolerant gene OsGSTU37 and the application have the advantages that knockout lines and over-expression lines of the rice cadmium-tolerant gene OsGSTU37 are constructed, as discovered in seed germination comparative experiments on the knockout lines, the over-expression lines and wild type rice, the knockout lines of the rice cadmium-tolerant gene OsGSTU37 are poor under cadmium stress, but the over-expression lines of the rice cadmium-tolerant gene OsGSTU37 and the wild type rice can germinate, and germination and growth of the over-expression lines of the rice cadmium-tolerant gene OsGSTU37are superior to germination and growth of the wild type rice; important effects of the rice cadmium-tolerant gene OsGSTU37 in cadmium-tolerant mechanisms can be equivalently described; over-expression vectors of the rice cadmium-tolerant gene OsGSTU37 are constructed, and plants are transformed by the aid of the over-expression vectors, so that cadmium-tolerant plant varieties can be obtained, the cadmium stress resistance of the plants can be improved, and theoretical bases can be provided for breeding novel cadmium-tolerant crop varieties.

Peptides having general and specific binding affinities for the Src homology region 3 (SH3) domains of proteins are disclosed in the present invention. In particular, SH3 binding peptides have been isolated from phage-displayed random peptide libraries which had been screened for isolates that bind to bacterial fusion proteins having an SH3 domain and glutathione S-transferase (GST). Preferred peptides are disclosed which comprise a core 7-mer sequence (preferably, a consensus motif) and two or more, preferably at least six, additional amino acid residues flanking the core sequence, for a total length of 9, preferably at least 13, amino acid residues and no more than about 45 amino acid residues. Such peptides manifest preferential binding affinities for certain SH3 domains. The preferred peptides exhibit specific binding affinities for the Src-family of proteins. In vitro and in vivo results are presented which demonstrate the biochemical activity of such peptides.

The invention discloses high throughput screening of important schistosoma japonicum antigens and application of the antigens in schistosomiasis diagnosis. Specifically, the invention provides important schistosoma antigens and application thereof in schistosomiasis diagnosis and vaccine preparation. Based on a high throughput functional protein screening technology of glutathione S transferase (GST) fusion expression, 24 positive antigens capable of being identified by the serum of a schistosomiasis patient are acquired, wherein 8 antigens represent strong positive reaction. Schistosomiasis serologic detection shows that the positive antigens for detecting schistosoma have high sensitivity and specificity. The invention also provides a corresponding method and a kit for diagnosing and monitoring the schistosomiasis.

The present invention relates to methods for the diagnosis of subjects that have or are at risk of having Alzheimer's disease (AD). In particular the present invention identifies individuals who have or are at risk of having AD through measurement of the levels of Afamin in combination with at least one other biomarker such as Alpha-1-antichymotrypsin, Alpha-2- macroglobulin, ApoB 100, Complement C5, Serine threonine protein kinase TBK1 or Complement C3 in a fluid sample taken from a subject. Furthermore, genotype (ApoIipoprotein E or glutathione S-transferase Omega) may also be taken into consideration and used within classification algorithms to determine the probability of a subject having or being at risk of having AD.