91results about How to "Specific recognition ability" patented technology

The invention discloses T lymphocyte modified by double specific chimeric antigen receptors and a preparation method and application thereof. The surface of the T lymphocyte can express a specific chimeric antigen receptor EGFRvIII-CD3zeta and a specific chimeric antigen receptor PD-L1-4-1BB. An amino acid sequence of the specific chimeric antigen receptor EGFRvIII-CD3zeta is shown in SEQ ID NO.1; an amino acid sequence of the specific chimeric antigen receptor PD-L1-4-1BB is shown in SEQ ID NO.2. The T lymphocyte modified by the double specific chimeric antigen receptors can be used for specifically identifying and killing and simultaneously expressing the tumor cells of EGFRvIII-CD3zeta and PD-L1 antigens, and the specificity is very strong.

The invention belongs to the field of biological analysis and detection and relates to fluorescent probes for labelling and / or detecting lipid droplets in cells as well as preparation and applicationsof the fluorescent probes. The probes are synthesized with 4-bromo-1,8-naphthalic anhydride as an initial material and take 1,8-naphthalimide as a fluorophore, a C12 alkyl long chain as a specific lipid droplet positioning group and an N-containing group on the 4th site of a naphthalene ring as a fluorescence enhanced and stabilized group. The probes are simple to prepare and have high yield. Compared with existing Nile red and boron dipyrromethene lipid droplet fluorescent probes, the probes have high light stability and large stokes shift and are more suitable for fluorescence imaging. Theprobes have very important application value in cell labelling fluorescent imaging and biomedical fields.

The invention discloses a ratio-type variant receptor mercury ion fluorescent probe and its preparation method and use. The ratio-type variant receptor mercury ion fluorescent probe has the advantages of simple synthesis processes, good light stability, the largest emission wavelength of 550nm and good chemical stability under neutral conditions. Through amide functional group tautomerism, the receptor has a deformation capability of combination of an imidic acid isomer and mercury ions and combination of an amide isomer structure and other metal ions so that the receptor has mercury ion specific combination selectivity. The probe with the mercury ion produces fluorescence spectrum red shift to 633nm and the fluorescence spectrum is enhanced by 9 times. After bonding to other metal ions especially such as congener zinc ion and cadmium ions, the probe has no obvious fluorescence change and has mercury ion specific fluorescence signal selectivity. The probe realizes mercury ion specific ratio identification, can be used in detection of mercury ions in cells and has a wide application prospect in detection of mercury ions in other organisms and environments.

The invention discloses a fluorescent probe for detecting the content of glutathione (GSH) in blood, and a synthesis method and an application thereof. The synthesis steps of the probe are simple, and the light stability is good. In the detection process, the fluorescent probe firstly as a substrate is combined with glutathione S-transferase (GST), and undergoes a reaction with another specific substrate GSH of the GST to produce fluorescence, the fluorescence is enhanced by about 35 times, and the maximum emission wavelength is at the near infrared region (beyond 700 nm). Compared with conventional GSH fluorescent probes, the probe can specifically detect the content of the GSH in a complex system, and has the advantages of wide dynamic response window, high efficiency and the like. The probe realizes the specific and high-efficiency identification on the GSH in the complex environment, and has extremely important application value in the biological and medical fields.

The invention discloses a preparation method of a loofah sponge surface lead ion imprinted absorbing material and the use of the loofah sponge surface lead ion imprinted absorbing material in metal ion absorption and belongs to natural high polymer material modification. In the method, the loofah sponge natural high polymer material is used as a support, and the surface of the material is modified with a lead ion imprinted polymer. The main technical characteristics of the method include: adding acylated loofah sponge, lead-dithizone complex, 4-vinylpyridine, ethylene dimethacrylate and azodiisobutyronitrile into a chloroform medium, removing oxygen by argon in a certain proportion; introducing argon to expel oxygen; reacting in a water bath at a constant temperature of 50 to 70 DEG C, filtering and washing; and performing Soxhlet extraction by using 0.10mol / L solution of HNO3 for 12 hours, removing template lead ions, washing, drying and obtaining the material. The material has specific lead ion identification capacity, high selectivity, high absorption speed and high desorption capacity, is widely available and biodegradable, can be prepared by a simple process and has regeneration capacity and the advantages of environmental friendliness and the like.

The invention discloses antibody-modified fluorescent nanoparticles and application thereof in targeted imaging of cancer cells. The fluorescent nanoparticles are prepared via the steps of dissolving an amphiphilic polymer and a fluorescent compound shown by formula I in a water-soluble organic solvent, and adding to water under ultrasonic action; blowing nitrogen to liquid surface to remove the organic solvent by volatilizing so as to form nanoparticles to which the fluorescent compound is supported; adding an antibody, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimide into nanoparticle aqueous solution, and reacting to form amido bonds so as to prepare the fluorescent nanoparticles capable of targeted imaging. The traditional fluorescence imaging reagent has the defects such as quenching induced by aggregation, poor light stability and high cytotoxicity, while the fluorescent nanoparticles described herein have the advantages such as better detection results, high luminous efficiency, specific recognizing capacity, low cytotoxicity, good biocompatibility, and high light stability.

The invention discloses a preparation method of glucan gel surface Sudan red molecularly-imprinted adsorption material, and the application of the glucan gel surface Sudan red molecularly-imprinted adsorption material in selective adsorption and separation of Sudan red molecules in food analysis. The method takes glucan gel as a supporter, and Sudan red molecularly-imprinted polymer is modified on the surface of the supporter. The preparation method is mainly and technically characterized by comprising the steps of: adding Sudan red, ethylene glycol dimethacrylate, azodiisobutyronitrile and sulfydryl glucan gel according to a certain proportion; in acetonitrile medium, removing oxygen by inert gas, carrying out reaction at the constant temperature, filtering and washing; carrying out Soxhlet extraction for 8-12h by 0.4-0.8mol / L of hydrochloric acid-ethanol solution to remove Sudan red template molecules; and finally, washing and drying to obtain the glucan gel surface Sudan red molecularly-imprinted adsorption material. The material has specific recognition capability on the Sudan red molecules, is high in selectivity, rapid in adsorption speed, good in desorption performance, biodegradable and simple in technology, has regeneration capacity and has the advantages of being environment-friendly and the like.

The present invention provides a cadmium ion fluorescence probe, a preparation method and applications thereof. According to the present invention, the probe has specific binding selectivity and fluorescence response to cadmium ions; 4-amno-7-nirobenzoxadiazole is used as a fluorophore and amide DPA is used as a receptor in the probe, and the acceptor can have the variation ability through the tautomerism of the amide functional group, wherein the isomer of the imidic acid binds the cadmium ions, and the structure of the amide isomer binds other metal ions so as to provide the specific binding selectivity to the cadmium ions; and the unique identification on the cadmium ions by the probe is achieved, the obtained probe can be used for detecting the cadmium ions in cells, and can further be used for the cadmium ion detection in other organisms and environments due to the wide pH value working range.

The invention discloses an anti-CA125 carbohydrate antigen nano-antibody. The nano-antibody has three unique complementarity determining regions CDR1, CDR2 and CDR3. The invention also discloses an application of the nano-antibody in the preparation of tumor treatment drug and tumor diagnosis reagents. The anti-CA125 nano-body has highly specific recognizing and binding ability to the CA125, has aremarkable ADCC effect on ovarian cancer cells, and can accurately image tumors in mouse bodies.

The invention relates to a galangin molecular imprinting polymer, belongs to the technical field of new materials, and mainly solves the defects that a galangin separation method is poor in separation efficiency, low in recovery rate and high in cost. The technical scheme of the invention is that the galangin molecular imprinting polymer is prepared by the following steps: (1), dissolving a template molecule galangin and a functional monomer acrylamide in a pore forming agent, and fully mixing uniformly; then adding a cross-linking agent and an initiator, mixing uniformly, introducing N2 for deoxidation, sealing, and carrying out water bath polymerization at the temperature of 55-65 DEG C for 20-28 h to obtain a polymer; (2), grinding the polymer obtained in the step (1) into a powder and sieving, successively eluting with a methanol solution having acetic acid with the volume ratio of 10% and methanol, removing the template molecule galangin and residual acetic acid, carrying out vacuum drying to reach a constant weight, and thus obtaining the galangin molecular imprinting polymer. The galangin molecular imprinting polymer has the advantages of high selectivity, high affinity and high efficiency.

The invention discloses a preparation method of a dumbbell-shaped molecular imprinting stirring rod. The method comprises the following steps of: dipping on the dumbbell-shaped stirring rod in alkali and acid in turn for carrying out activation and silanization reaction; further dipping the dumbbell-shaped stirring rod into a trimethylolpropane tricrylic acid ester solution to carry out grafting reaction; putting a template molecule vardenafil and a functional monomer into a pore-foaming agent for prepolymerization, subsequently adding a cross-linking agent and an initiator and putting into the grafted dumbbell-shaped stirring rod to polymerize; repeating the polymerization reaction of the dumbbell-shaped stirring rod, further aging, and finally washing the template molecules so as to obtain the dumbbell-shaped molecular imprinting stirring rod. The stirring rod prepared by using the preparation method is combined with a chromatograph and can be applied to rapid separation and enrichment of nafil compounds in complex matrixes such as food, medicines and health-care products, so that accurate detection on a sample, with high selectivity and high sensitivity, is achieved.

The invention provides a chimeric antigen receptor (CAR) and a CAR encoding gene. The CAR comprises an extracellular structural domain capable of binding antigens, a transmembrane structural domain and intracellular immune costimulatory molecules, wherein the extracellular structural domain comprises a D2 structural domain of a Slit2 protein. In addition the invention also provides a chimeric antigen receptor expression cell, and the CAR encoding gene is introduced into the cell, so that the CAR is expressed on the surface of the cell. The CAR or the CAR expression cell provided by the invention can be used as cell medicines in the treatment of tumor diseases. The CAR provided by the invention can be used to modify cells, especially T cells, and modified T cells can specifically recognize and kill or wound tumors and have more efficient tumor killing activity.

The invention relates to a nanometer antibody for resisting salmonella enteritidis and application thereof. The antibody has the function of being combined with the salmonella enteritidis. The invention also discloses the nanometer antibody and a gene sequence for coding the nanometer antibody, an expression carrier and a host cell of the nanometer antibody, and a method for producing the nanometer antibody. The nanometer antibody for resisting the salmonella enteritidis has the advantages that the size is small; the expression efficiency is high; the dissolution performance is good; the stability is high, and the like. The invention also provides the application of the nanometer antibody for resisting the salmonella enteritidis.

The invention discloses a anti-procalcitonin high-affinity nano antibody. The nano antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing the variable region coding sequence of the nano antibody, and a host cell containing the expression vector. The invention further provides a fusion protein of the nano antibody variable region and alkaline phosphatase, the application of the nano antibody in preparation of a procalcitonin detection kit, a method for carrying out procalcitonin immunodetection by using thenano antibody, and a corresponding detection kit. The anti-procalcitonin nano antibody provided by the invention has specific recognition and binding capacity on procalcitonin. The affinity of the nano antibody can reach 3.773 E to 9. The nano antibody has a unique antigenic determinant recognition site, and can acquire an excellent detection effect in procalcitonin detection, and especially in adouble-antibody sandwich method.

The invention discloses an anti-AFP nanometer antibody. The nanometer antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention also provides an expression carrier which contains a nanometer antibody variable region coding sequence, a host cell containing the expression carrier, and application of the nanometer antibody in preparation of tumor preparation medicines and tumor diagnosis reagents. The nanometer antibody provided by the invention has a specific recognizing and binding capacity for AFP, the affinity of the nanometer antibody can be 3.082E-09, the nanometer antibody has a unique antigenic determinant recognition site and has an obvious ADCC effect for cancer cells, and an excellent detection effect can be obtained in AFP serum detection, especially in a double-antibody sandwich method.

The present invention discloses a sunset yellow molecular imprinting detection electrochemical sensor preparation method. In the method, magnetic graphene is used as a carrier, the surface is modified with 1-butyl-3-methyl imidazole hydrobromide ionic liquid and nano Au, and a sunset yellow molecular imprinting polymer is prepared by addition of a sunset yellow template molecule, a functional monomer and a crosslinking agent on the basis of the polymer. The glassy carbon electrode surface is modified with the sunset yellow molecular imprinting polymer for construction of a sunset yellow molecular imprinting detection electrochemical sensor. The electrochemical sensor constructed on the basis of the imprinted material has high sunset yellow detection selectivity and sensitivity, and can achieve rapid and sensitive detection of a real sample.

The invention discloses an anti-HE4 nano antibody, the nano antibody has three unique complementary determining regions CDR1, CDR2 and CDR3, the invention also provides an expression vector containing a encoding sequence of a variable region of the nano antibody, a host cell containing the expression vector, and an application of the nano antibody in detection of HE4 content in serum. The nano antibody provided by the invention has specific recognition and binding ability to HE4, the affinity of the nano antibody can reach 1.009 E-09, and the nano antibody has a unique antigenic determinant recognition site and can obtain an excellent detection effect in HE4 serum detection by using a double-antibody sandwich method.

The invention discloses an anti-HE4 nanobody, the nanobody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention also provides an expression vector containing a coding sequence of a variable region of the nano-antibody, a host cell containing the expression vector and application of the nano antibody in detecting HE4 levels in serum. The nanobody provided by the invention has specific recognition and binding capacities to HE4, the affinity of the nanobody can reach 2.773E-09; and the nanobody has a unique antigenic determinant recognition site, so that an excellent detection effect in HE4 serum detection can be obtained, especially in a double-antibody sandwich method.

The invention discloses an anti-HE4 nanobody, the nanobody has three unique complementary determining regions CDR1, CDR2 and CDR3, the invention also provides an expression vector containing a coding sequence of a variable region of the nanobody, a host cell containing the expression vector, and application of the nanobody in detection of HE4 levels in serum. The nanobody provided by the invention has specific recognition and binding capacities to HE4, and the affinity of the nanobody can reach 7.020 E-10; and the nanobody has a unique antigenic determinant recognition site, so that an excellent detection effect in HE4 serum detection can be obtained, especially in a double-antibody sandwich method.

The invention discloses an anti-procalcitonin nano antibody. The anti-procalcitonin nano antibody has three unique complementary determining regions CDR1, CDR2, and CDR3. The invention also provides an expression vector containing the variable region coding sequence of the nano antibody, host cells containing the expression vector, a fusion protein of the nano antibody and alkaline phosphatase, and an application of the nano antibody in preparation of a procalcitonin detection kit. The anti-procalcitonin nano antibody provided by the invention has specific recognition and binding capacity on procalcitonin, the affinity with antigen can reach 7.429 E-9, and an excellent detection effect is obtained in procalcitonin detection, especially in application of a double-antibody sandwich method.

The present invention discloses a preparation method of a porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the preparation method, waste ceramic is used as the supporting body of an adsorbent, and perfluorooctane sulfonic acid is used as a template molecule; 58-65% by mass of N,N-dimethylformamide, 15-20% by mass of succinimide, 1.0-3.0% by mass of perfluorooctane sulfonic acid, 12-18% by mass of aminated porous ceramic powder, and 1.0-4.0% by mass of ammonium persulfate are added to a reactor, a stirring reaction is performed for 6 h at a temperature of 75+ / -2 DEG C in the absence of oxygen, the obtained product is subjected to stirring washing for 12 h with the mixed solution of ethanol and sodium hydroxide, the template molecule is removed, and drying is performed to obtain the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the present invention, the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent can specifically recognize perfluorooctane sulfonic acid, and has advantages of high selectivity, good mechanical property, good chemical stability, fast adsorption, easy elution, and regenerative capacity.

The invention discloses a single domain antibody for resisting CEACAM-5. The single domain antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing a variable region coding sequence of the single domain antibody, a host cell containing the expression vector, fusion protein of a variable region of the single domain antibody and human alkaline phosphatase, application of the single domain antibody in preparing a CEACAM-5 detection kit, a method for CEACAM-5 immunological detection by applying the single domainantibody as well as a corresponding detection kit. The single domain antibody for resisting the CEACAM-5, provided by the invention, has specific recognizing and binding capability for the CEACAM-5;the affinity of the single domain antibody can reach 4.51E-09; the single domain antibody has unique antigenic determinant recognition site and can obtain an excellent detection result in CEACAM-5 immunological detection, in particular to a double antibody sandwich method.

The invention provides a temperature-pH responsive molecularly-imprinted fiber membrane and a preparation method thereof, belonging to the field of polymer functional materials. The preparation method of the temperature-pH responsive molecularly-imprinted fiber membrane comprises the following steps: preparing a nanofiber membrane from chitosan by using an electrostatic spinning technology, and then grafting a temperature responsive polymer poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)-co-methacrylic acid on the surface of the nanofiber membrane through a free radical polymerization reaction. A prepared material has specific recognition capacity on dyes or heavy metal ions, the maximum adsorption capacity of the material on gentian violet dyes reaches 212.0 mg / g, adsorption equilibrium time is 7 min, and an desorption rate reaches 97%. The morphology of the fiber membrane is kept in a solution with a pH value of 6.0-14.0; the fiber membrane is dissolved in a solution with a pH value of 1.0-6.0; the molecular chain of a polymer brush is hydrophilic and is stretched when the temperature is lower than 32.5 DEG C; and the molecular chain is curled up and separated out from water when the temperature is higher than 32.5 DEG C. After adsorption is finished, pollutants can be easily and rapidly separated out due to temperature and pH responsiveness, so effective removal and enrichment of the pollutants are realized. The fiber membrane has a good application prospect in the aspects of environmental protection and water quality detection and analysis.

The present invention discloses an anti-anthrax PA antigen monoclonal antibody having a humanize form, a chimeric form and a murine form, and applications of the monoclonal antibody in anthrax treatment drug preparation. The anti-anthrax PA antigen monoclonal antibody has characteristics of specific antigen binding, excellent toxin neutralization activity and good animal protection function.

The invention provides a 17 beta-estradiol molecular imprinting titanium dioxide (TiO2) nano tube and a preparation method thereof. The TiO2 of molecular imprinting can perform selective adsorption and photocatalytic degradation on the 17 beta-estradiol in a water body. The molecular imprinting nano tube is formed by a TiO2 nano tube with holes at two ends and a molecular imprinting polymer, and the mass ratio of the TiO2 nano tube to the molecular imprinting polymer is 63 to 37. The molecular imprinting polymer is prepared by methacrylic acid, trimethylolpropane trimethyl acrylic ester and azo dicyano pentanoic acid according to the molar ratio of 124:123:1. Carbonyl function functional groups are imprinted on the molecular imprinting polymer. The diameter of the molecular imprinting TiO2 nano tube is 90nm, the length is 600nm, the porosity is 0.54cm3 / g, and the specific surface area is 300m2 / g. The molar ratio of functional monomer methyl acrylic to template molecule estradiol is 124 to 26. The invention further provides the preparation method and application of the 17 beta-estradiol molecular imprinting TiO2 nano tube. The 17 beta-estradiol molecular imprinting TiO2 nano tube is good in estradiol adsorption, has the degradation rate as high as 98%, and can play a positive role on improving sewage reuse water quality.

The invention discloses a monoclonal antibody resistant to different structural domains of a SasA (staphylococcus aureus surface protein A) antigen as well as an application of the monoclonal antibody in preparation of a drug for resisting staphylococcus aureus infection. The specific antibody has good protection performance in a mouse model when in combined utilization.

The invention provides a cadmium-ion fluorescence probe based on a pyrene exciplex, and a preparation method and application thereof. The probe has a structure as described in the specification. The preparation method comprises the following steps: dissolving 1-aminopyrene in CH2Cl2, then adding DMAP and slowly adding chloroacetyl chloride drop by drop under an ice-bath condition; allowing the temperature of a mixture obtained in the previous step to slowly rise to room temperature, removing the solvent through rotary evaporation after completion of a reaction, and loading a reaction product into a silica gel column; adding acetonitrile into obtained 1-(2-chloroacetyl)pyrene, then adding KI and DIPEA and slowly adding DPA drop by drop; and carrying out a reflux reaction, carrying out spin-drying to remove the solvent after completion of the reaction and treating a product of the reflux reaction with the silica gel column. The probe provided by the invention can emit fluorescent response signals specific to cadmium ions and is applicable to detection of cadmium ions in environment.

The invention discloses a squaric acid dye and application thereof in manufacturing a colorimetric fluorescent dual-response copper ion probe. The preparation method comprises the following steps: (1)mixing a phenylamine derivative with squaric acid to obtain a mixture, dissolving the mixture into a proper solvent, connecting to an upper water distributor, and performing decompressing inner ebullition and separating water for several hours under protection of N2; (2) cooling a reaction mixture obtained in step (1) to the room temperature, and removing the solvent under reduced pressure to obtain a crude product; and (3) performing chromatographic purification on the crude product through a silicagel column, thereby obtaining a near-infrared squaric acid dye product. The squaric acid dye is good in stability and is excellent in optical performance; a nitrogen atom and an oxygen atom on a side chain have relatively good bonding force with copper ions, and only copper ion in various added metal ions in the system causes fluorescence quenching of the dye, and the color of the solution is changed to colorless from blue color, and therefore, the dye can be taken as a colorimetric / fluorescent probe for copper ion detection, can be used for detecting copper ion in a water-containing medium, and shows relatively good detection sensitivity.

The invention discloses a preparation method of a molecular imprinting adsorption material for kaempferide on the surface of cotton bast. The preparation method is characterized by comprising the following steps: modifying the surface of the cotton bast through gamma-trimethoxysilyl propyl methacrylate, thus obtaining surface-modified cotton bast; adding 50 to 60 percent of ethyl alcohol, 8 to 14 percent of ethylene glycol dimethacrylate, 4 to 8 percent of 4-vinyl pyridine, 3.0 to 8.0 percent of the kaempferide, 15 to 22 percent of the surface-modified cotton bast and 1.0 to 3.0 percent of azodiisobutyronitrile into a reactor, stirring and dissolving the components, feeding an inert anaerobic atmosphere, performing stirring reaction, and removing template modules with a methyl alcohol and acetic acid mixing solution, thus obtaining the molecular imprinting adsorption material for the kaempferide on the surface of the cotton bast. The adsorption material has specific identification capacity for the kaempferide, is relatively high in selectivity, high in mechanical property, extremely high in chemical stability, high in adsorption speed, easy to elute, biodegradable and simple in process, and has the advantages of regeneration capacity, environment friendliness and the like.

The invention discloses a method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling, relating to the scientific and technical fields of material science, self-assembling, fluorescence analysis and detection and the like. According to the method, hydrophobic fluorescent monomers and hydrophilic monomers are copolymerized and are modified by virtue of monomers containing double bonds so as to generate a photosensitive fluorescent amphophilic polymer; the molecular imprinting fluorescent nanoparticles are prepared through macromolecule self-assembling and photo-crosslinking, and the specific recognition and detection of a molecular imprinting material to template molecules in a water phase are realized; and meanwhile, the material is endowed with a relatively large specific surface area by virtue of a nano-structure, so that the material has more active binding sites and is relatively high in mass transfer rate and bindingkinetics. By combining high sensitivity of fluorescence detection, the selectivity of a molecular imprinting technique and macromolecule self-assembling, the obtained molecular imprinting fluorescentnanoparticles can be applied to high-selectivity and high-sensitivity rapid detection of trace micromolecules or biomacromolecules in an actual sample and particularly have relatively large utilization potentials in the fields of food safety detection, environmental and water-pollution detection, clinical analysis and the like.