90results about How to "Specific recognition ability" patented technology

The invention discloses T lymphocyte modified by double specific chimeric antigen receptors and a preparation method and application thereof. The surface of the T lymphocyte can express a specific chimeric antigen receptor EGFRvIII-CD3zeta and a specific chimeric antigen receptor PD-L1-4-1BB. An amino acid sequence of the specific chimeric antigen receptor EGFRvIII-CD3zeta is shown in SEQ ID NO.1; an amino acid sequence of the specific chimeric antigen receptor PD-L1-4-1BB is shown in SEQ ID NO.2. The T lymphocyte modified by the double specific chimeric antigen receptors can be used for specifically identifying and killing and simultaneously expressing the tumor cells of EGFRvIII-CD3zeta and PD-L1 antigens, and the specificity is very strong.

The invention discloses antibody-modified fluorescent nanoparticles and application thereof in targeted imaging of cancer cells. The fluorescent nanoparticles are prepared via the steps of dissolving an amphiphilic polymer and a fluorescent compound shown by formula I in a water-soluble organic solvent, and adding to water under ultrasonic action; blowing nitrogen to liquid surface to remove the organic solvent by volatilizing so as to form nanoparticles to which the fluorescent compound is supported; adding an antibody, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimide into nanoparticle aqueous solution, and reacting to form amido bonds so as to prepare the fluorescent nanoparticles capable of targeted imaging. The traditional fluorescence imaging reagent has the defects such as quenching induced by aggregation, poor light stability and high cytotoxicity, while the fluorescent nanoparticles described herein have the advantages such as better detection results, high luminous efficiency, specific recognizing capacity, low cytotoxicity, good biocompatibility, and high light stability.

The invention relates to a galangin molecular imprinting polymer, belongs to the technical field of new materials, and mainly solves the defects that a galangin separation method is poor in separation efficiency, low in recovery rate and high in cost. The technical scheme of the invention is that the galangin molecular imprinting polymer is prepared by the following steps: (1), dissolving a template molecule galangin and a functional monomer acrylamide in a pore forming agent, and fully mixing uniformly; then adding a cross-linking agent and an initiator, mixing uniformly, introducing N2 for deoxidation, sealing, and carrying out water bath polymerization at the temperature of 55-65 DEG C for 20-28 h to obtain a polymer; (2), grinding the polymer obtained in the step (1) into a powder and sieving, successively eluting with a methanol solution having acetic acid with the volume ratio of 10% and methanol, removing the template molecule galangin and residual acetic acid, carrying out vacuum drying to reach a constant weight, and thus obtaining the galangin molecular imprinting polymer. The galangin molecular imprinting polymer has the advantages of high selectivity, high affinity and high efficiency.

The invention discloses a anti-procalcitonin high-affinity nano antibody. The nano antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing the variable region coding sequence of the nano antibody, and a host cell containing the expression vector. The invention further provides a fusion protein of the nano antibody variable region and alkaline phosphatase, the application of the nano antibody in preparation of a procalcitonin detection kit, a method for carrying out procalcitonin immunodetection by using thenano antibody, and a corresponding detection kit. The anti-procalcitonin nano antibody provided by the invention has specific recognition and binding capacity on procalcitonin. The affinity of the nano antibody can reach 3.773 E to 9. The nano antibody has a unique antigenic determinant recognition site, and can acquire an excellent detection effect in procalcitonin detection, and especially in adouble-antibody sandwich method.

The invention discloses an anti-AFP nanometer antibody. The nanometer antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention also provides an expression carrier which contains a nanometer antibody variable region coding sequence, a host cell containing the expression carrier, and application of the nanometer antibody in preparation of tumor preparation medicines and tumor diagnosis reagents. The nanometer antibody provided by the invention has a specific recognizing and binding capacity for AFP, the affinity of the nanometer antibody can be 3.082E-09, the nanometer antibody has a unique antigenic determinant recognition site and has an obvious ADCC effect for cancer cells, and an excellent detection effect can be obtained in AFP serum detection, especially in a double-antibody sandwich method.

The present invention discloses a sunset yellow molecular imprinting detection electrochemical sensor preparation method. In the method, magnetic graphene is used as a carrier, the surface is modified with 1-butyl-3-methyl imidazole hydrobromide ionic liquid and nano Au, and a sunset yellow molecular imprinting polymer is prepared by addition of a sunset yellow template molecule, a functional monomer and a crosslinking agent on the basis of the polymer. The glassy carbon electrode surface is modified with the sunset yellow molecular imprinting polymer for construction of a sunset yellow molecular imprinting detection electrochemical sensor. The electrochemical sensor constructed on the basis of the imprinted material has high sunset yellow detection selectivity and sensitivity, and can achieve rapid and sensitive detection of a real sample.

The invention discloses an anti-HE4 nano antibody, the nano antibody has three unique complementary determining regions CDR1, CDR2 and CDR3, the invention also provides an expression vector containing a encoding sequence of a variable region of the nano antibody, a host cell containing the expression vector, and an application of the nano antibody in detection of HE4 content in serum. The nano antibody provided by the invention has specific recognition and binding ability to HE4, the affinity of the nano antibody can reach 1.009 E-09, and the nano antibody has a unique antigenic determinant recognition site and can obtain an excellent detection effect in HE4 serum detection by using a double-antibody sandwich method.

The invention discloses an anti-HE4 nanobody, the nanobody has three unique complementary determining regions CDR1, CDR2 and CDR3, the invention also provides an expression vector containing a coding sequence of a variable region of the nanobody, a host cell containing the expression vector, and application of the nanobody in detection of HE4 levels in serum. The nanobody provided by the invention has specific recognition and binding capacities to HE4, and the affinity of the nanobody can reach 7.020 E-10; and the nanobody has a unique antigenic determinant recognition site, so that an excellent detection effect in HE4 serum detection can be obtained, especially in a double-antibody sandwich method.

The invention discloses an anti-procalcitonin nano antibody. The anti-procalcitonin nano antibody has three unique complementary determining regions CDR1, CDR2, and CDR3. The invention also provides an expression vector containing the variable region coding sequence of the nano antibody, host cells containing the expression vector, a fusion protein of the nano antibody and alkaline phosphatase, and an application of the nano antibody in preparation of a procalcitonin detection kit. The anti-procalcitonin nano antibody provided by the invention has specific recognition and binding capacity on procalcitonin, the affinity with antigen can reach 7.429 E-9, and an excellent detection effect is obtained in procalcitonin detection, especially in application of a double-antibody sandwich method.

The present invention discloses a preparation method of a porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the preparation method, waste ceramic is used as the supporting body of an adsorbent, and perfluorooctane sulfonic acid is used as a template molecule; 58-65% by mass of N,N-dimethylformamide, 15-20% by mass of succinimide, 1.0-3.0% by mass of perfluorooctane sulfonic acid, 12-18% by mass of aminated porous ceramic powder, and 1.0-4.0% by mass of ammonium persulfate are added to a reactor, a stirring reaction is performed for 6 h at a temperature of 75+/-2 DEG C in the absence of oxygen, the obtained product is subjected to stirring washing for 12 h with the mixed solution of ethanol and sodium hydroxide, the template molecule is removed, and drying is performed to obtain the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the present invention, the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent can specifically recognize perfluorooctane sulfonic acid, and has advantages of high selectivity, good mechanical property, good chemical stability, fast adsorption, easy elution, and regenerative capacity.

The invention discloses a single domain antibody for resisting CEACAM-5. The single domain antibody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing a variable region coding sequence of the single domain antibody, a host cell containing the expression vector, fusion protein of a variable region of the single domain antibody and human alkaline phosphatase, application of the single domain antibody in preparing a CEACAM-5 detection kit, a method for CEACAM-5 immunological detection by applying the single domainantibody as well as a corresponding detection kit. The single domain antibody for resisting the CEACAM-5, provided by the invention, has specific recognizing and binding capability for the CEACAM-5;the affinity of the single domain antibody can reach 4.51E-09; the single domain antibody has unique antigenic determinant recognition site and can obtain an excellent detection result in CEACAM-5 immunological detection, in particular to a double antibody sandwich method.

The invention provides a temperature-pH responsive molecularly-imprinted fiber membrane and a preparation method thereof, belonging to the field of polymer functional materials. The preparation method of the temperature-pH responsive molecularly-imprinted fiber membrane comprises the following steps: preparing a nanofiber membrane from chitosan by using an electrostatic spinning technology, and then grafting a temperature responsive polymer poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)-co-methacrylic acid on the surface of the nanofiber membrane through a free radical polymerization reaction. A prepared material has specific recognition capacity on dyes or heavy metal ions, the maximum adsorption capacity of the material on gentian violet dyes reaches 212.0 mg/g, adsorption equilibrium time is 7 min, and an desorption rate reaches 97%. The morphology of the fiber membrane is kept in a solution with a pH value of 6.0-14.0; the fiber membrane is dissolved in a solution with a pH value of 1.0-6.0; the molecular chain of a polymer brush is hydrophilic and is stretched when the temperature is lower than 32.5 DEG C; and the molecular chain is curled up and separated out from water when the temperature is higher than 32.5 DEG C. After adsorption is finished, pollutants can be easily and rapidly separated out due to temperature and pH responsiveness, so effective removal and enrichment of the pollutants are realized. The fiber membrane has a good application prospect in the aspects of environmental protection and water quality detection and analysis.

The present invention discloses an anti-anthrax PA antigen monoclonal antibody having a humanize form, a chimeric form and a murine form, and applications of the monoclonal antibody in anthrax treatment drug preparation. The anti-anthrax PA antigen monoclonal antibody has characteristics of specific antigen binding, excellent toxin neutralization activity and good animal protection function.

The invention provides a 17 beta-estradiol molecular imprinting titanium dioxide (TiO2) nano tube and a preparation method thereof. The TiO2 of molecular imprinting can perform selective adsorption and photocatalytic degradation on the 17 beta-estradiol in a water body. The molecular imprinting nano tube is formed by a TiO2 nano tube with holes at two ends and a molecular imprinting polymer, and the mass ratio of the TiO2 nano tube to the molecular imprinting polymer is 63 to 37. The molecular imprinting polymer is prepared by methacrylic acid, trimethylolpropane trimethyl acrylic ester and azo dicyano pentanoic acid according to the molar ratio of 124:123:1. Carbonyl function functional groups are imprinted on the molecular imprinting polymer. The diameter of the molecular imprinting TiO2 nano tube is 90nm, the length is 600nm, the porosity is 0.54cm3/g, and the specific surface area is 300m2/g. The molar ratio of functional monomer methyl acrylic to template molecule estradiol is 124 to 26. The invention further provides the preparation method and application of the 17 beta-estradiol molecular imprinting TiO2 nano tube. The 17 beta-estradiol molecular imprinting TiO2 nano tube is good in estradiol adsorption, has the degradation rate as high as 98%, and can play a positive role on improving sewage reuse water quality.

The invention discloses a monoclonal antibody resistant to different structural domains of a SasA (staphylococcus aureus surface protein A) antigen as well as an application of the monoclonal antibody in preparation of a drug for resisting staphylococcus aureus infection. The specific antibody has good protection performance in a mouse model when in combined utilization.

The invention provides a cadmium-ion fluorescence probe based on a pyrene exciplex, and a preparation method and application thereof. The probe has a structure as described in the specification. The preparation method comprises the following steps: dissolving 1-aminopyrene in CH2Cl2, then adding DMAP and slowly adding chloroacetyl chloride drop by drop under an ice-bath condition; allowing the temperature of a mixture obtained in the previous step to slowly rise to room temperature, removing the solvent through rotary evaporation after completion of a reaction, and loading a reaction product into a silica gel column; adding acetonitrile into obtained 1-(2-chloroacetyl)pyrene, then adding KI and DIPEA and slowly adding DPA drop by drop; and carrying out a reflux reaction, carrying out spin-drying to remove the solvent after completion of the reaction and treating a product of the reflux reaction with the silica gel column. The probe provided by the invention can emit fluorescent response signals specific to cadmium ions and is applicable to detection of cadmium ions in environment.

The invention discloses a squaric acid dye and application thereof in manufacturing a colorimetric fluorescent dual-response copper ion probe. The preparation method comprises the following steps: (1)mixing a phenylamine derivative with squaric acid to obtain a mixture, dissolving the mixture into a proper solvent, connecting to an upper water distributor, and performing decompressing inner ebullition and separating water for several hours under protection of N2; (2) cooling a reaction mixture obtained in step (1) to the room temperature, and removing the solvent under reduced pressure to obtain a crude product; and (3) performing chromatographic purification on the crude product through a silicagel column, thereby obtaining a near-infrared squaric acid dye product. The squaric acid dye is good in stability and is excellent in optical performance; a nitrogen atom and an oxygen atom on a side chain have relatively good bonding force with copper ions, and only copper ion in various added metal ions in the system causes fluorescence quenching of the dye, and the color of the solution is changed to colorless from blue color, and therefore, the dye can be taken as a colorimetric/fluorescent probe for copper ion detection, can be used for detecting copper ion in a water-containing medium, and shows relatively good detection sensitivity.

The invention discloses a method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling, relating to the scientific and technical fields of material science, self-assembling, fluorescence analysis and detection and the like. According to the method, hydrophobic fluorescent monomers and hydrophilic monomers are copolymerized and are modified by virtue of monomers containing double bonds so as to generate a photosensitive fluorescent amphophilic polymer; the molecular imprinting fluorescent nanoparticles are prepared through macromolecule self-assembling and photo-crosslinking, and the specific recognition and detection of a molecular imprinting material to template molecules in a water phase are realized; and meanwhile, the material is endowed with a relatively large specific surface area by virtue of a nano-structure, so that the material has more active binding sites and is relatively high in mass transfer rate and bindingkinetics. By combining high sensitivity of fluorescence detection, the selectivity of a molecular imprinting technique and macromolecule self-assembling, the obtained molecular imprinting fluorescentnanoparticles can be applied to high-selectivity and high-sensitivity rapid detection of trace micromolecules or biomacromolecules in an actual sample and particularly have relatively large utilization potentials in the fields of food safety detection, environmental and water-pollution detection, clinical analysis and the like.