638results about How to "Easy to elute" patented technology

A method of intra-articular drug delivery may include selecting an attachment zone in a synovial joint; affixing a drug release device in the attachment zone, the drug release device comprising a base affixable in the attachment zone, a sustained-release drug carrier, and a drug, the device positioned so that the device releases the drug into the synovial fluid of the synovial joint, and so that agitation of the synovial fluid facilitates elution of the drug from the drug release device.

A stent is provided with a series of short pieces or sections connected together by at least one polymer fiber or wire. The polymer fiber or wire can be biodegradable or durable. The fiber polymers may also contain beads, blobs and bulges in the fibers. The stent sections are designed to separate or articulate with time as the body lumen moves in response to biological and physiological events.

The current landscape for preparative chromatographic RNA purification uses reversed phase HPLC, but this technique presents many issues with process scale up and ion exchange for preparative purification has only been used for short RNAs. The invention provides preparative purification of RNA (e.g., mRNA) using ion (e.g., anion) exchange chromatography that allows for separation of longer RNAs up to 10,000 nucleotides in length via a scalable method. This method avoids problems with current techniques by using low pressure chromatography that is agreeable with existing equipment in cGMP commercial facilities, that uses aqueous-bases solutions as the mobile phase (rather than flammable of greater than 10 mg RNA / mL resin (e.g., using larger pore sorbents, >500 Angstroms, that display greater mRNA binding capacities), and that yields desired RNA salt forms for downstream formulation with no additional manipulation necessary (unlike ion pair reverse phase techniques).

A minicell display method has been developed which has significant advantages for screening peptide libraries for candidates that can bind and effectively modulate a particular biological process. The method, based on the small, anucleate minicell, has increased versatility in generating unique sequences to screen as well as increasing the size of the peptides to be screened. In vivo mutagenesis, at the level of protein synthesis, as well as DNA replication, increases diversification of the library to be screened and therefore substantially increases the number of potential peptides that can modulate a particular biological response or mechanism.

A method of intra-articular drug delivery may include selecting an attachment zone in a synovial joint; affixing a drug release device in the attachment zone, the drug release device comprising a base affixable in the attachment zone, a sustained-release drug carrier, and a drug, the device positioned so that the device releases the drug into the synovial fluid of the synovial joint, and so that agitation of the synovial fluid facilitates elution of the drug from the drug release device.

The invention provides a preparation method of an adsorption material with high selectivity on different heavy metal ions. The invention relates to a method of producing a reusable adsorption material with selectivity on such heavy metal ions as copper, chromium, lead, mercury, and the like, wherein inorganic minerals such as montmorillonite and silica are used as modified carriers, and the method comprises the following steps of: 1) pre-treating and thermally activating the carriers; 2) performing surface polymerizing on a template ion carrier; 3) performing crosslinking film covering technology on carrier surface imprinting molecules. The materials can be applied in treatment processes of mixed solutions, production water and wastewater for separating, removing and enriching specific heavy metal ions.

The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.

Embodiments of the invention provide devices, methods and systems for performing microfluidic chromatography. Particular embodiments provide microfluidic chromatography column devices which can perform chemical separation using small sample volumes and low pressure differentials across the column. One embodiment provides a microfluidic chromatography column device comprising a first, second and third capillary tube. A chromatographic packing is disposed in the second tube with a first and second support layer disposed on opposite ends of the second tube. The support layers are disposed in a substantially flat orientation within the tube. An external coupling joins the tubes such that the tubes are fluidically sealed. The device is configured to have a fluidic resistance such that a pressure differential across the column of less than about 10 psi produces a flow rate through the device of at least about 0.5 ml / min for a liquid solution.

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention discloses a method for preparing bisphenol A nanometer silicon gel carrier surface molecule print solid phase extraction filling. Firstly, silanizing agent containing double bonds is grafted on the surfaces of nanometer silicon dioxide beads; secondly, template molecules, functional monomers and crosslinking agents are added into toluene solution containing proper grafted nanometer silicon and catalytic amount evocating agent according to a certain proportion and stirred for polyreaction at temperature of 60 to 70 DEG C and for 15 to 24 hours to generate light-yellow powdery polymer; and finally, templates are eluted from the polymer, and the bisphenol A nanometer silicon gel carrier surface molecule print solid phase extraction filling which has high special selectivity to bisphenol A is obtained. Because the invention adopts the tetrabromobisphenol A and biphenyl diphenol which are analogues of target molecule BPA of as templates, the problem of template leakage is solved. The prepared bisphenol A nanometer silicon gel carrier surface molecule print solid phase extraction filling has high special selectivity to bisphenol A, can collect bisphenol A molecules in trace amount of water sample, and has high recovery ratio and good reproducibility.

The invention discloses an active carbon fiber and a preparation method thereof. The method comprises the following steps: obtaining a benzoxazine solution; obtaining a mixed solution of blending polymers; obtaining a composite nanofiber; and preparing the active carbon fiber. The preparation method is simple, the kinds and the application amounts of reaction reagents required by the method are less, and the cost is low. The active carbon fiber prepared through the method has the advantages of extremely-high specific surface area, good fiber diameter and aperture uniformity, excellent mechanical performances and excellent heat stability.

The invention relates to a magnetic bionic adsorbent and an application of the magnetic bionic adsorbent in treating acid wastewater containing uranium. A solvothermal method for preparing Fe3O4 by taking PSSMA (poly (4-styrene sulfonate-copolymer-maleic acid) sodium salt) as a surfactant is improved, grain sizes are uniform, and Fe3O4 magnetic nanoparticles can be synthesized on a large scale; biomolecule dopamine is polymerized in a trihydroxymethyl aminomethane buffer system and coats the surfaces of the Fe3O4 magnetic nanoparticles, and the novel magnetic bionic adsorbent with a core-shell structure is established. The magnetic bionic adsorbent has excellent hydrophilicity and larger specific surface area due to the the polydopamine structure of a shell layer, rich functional groups on the surface of the magnetic bionic adsorbent have high uranium adsorption capacity in a wider acidity range, the magnetic bionic adsorbent has higher uranium adsorption capacity and good selectivity, and uranium adsorbed on the surface of the adsorbent can be easily washed with a 0.1 mol / L (NH4)2CO3 solution and can be recycled conveniently.

The invention discloses a mesoporous molecular sieve SBA-15 composite nano surface imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: dispersing mesoporous molecular sieve SBA-15 powder into a methylbenzene solution in an ultrasonic mode, further adding methylacryloyl acyloxy propyl triethoxy silane so as to prepare a modified mesoporous molecular sieve SBA-15, further washing by using methylbenzene and methanol in sequence in the ultrasonic mode, centrifuging, and finally drying in vacuum for later use; adding template molecules and functional monomers into a pore-foaming agent, and dissolving in the ultrasonic mode to prepare a pre-assembled solution A; dispersing the modified mesoporous molecular sieve into the pore-foaming agent so as to obtain a solution B; mixing the solutions, further adding a cross-linking agent and an initiating agent, and raising the temperature to perform polymerization reaction; and after the reaction, centrifuging to remove the supernate, repeatedly washing a product by using a methanol-glacial acetic acid mixture solution in the ultrasonic mode, and further washing by using methanol to be neutral so as to obtain the mesoporous molecular sieve SBA-15 composite nano surface imprinted polymer. Due to adoption of the polymer, high-efficient separation and purification of in-vivo baicalin are achieved.

The invention relates to a method and apparatus for detecting elution of a sample from a sample substrate to incubation buffer contained in a sample well while the sample substrate is still within the well. The method comprises measuring light absorption of the contents of the sample well at a predefined wavelength or wavelength range, and determining, based on the absorption measurement, the degree of elution of the sample. According to the invention, a wavelength or wavelength range is used which is absorbed by at least one elutable component of the sample but transmitted by the sample substrate. The invention provides a reliable way of determining the degree of elution of blood samples in neonatal screening, for example.

A device, comprising an NO eluting polymer is provided. Said device is furthermore provided with microencapsulated liquid (101), such as water or water containing liquid in micro-capsules (101), which water or water containing liquid after breakage of said micro capsules, initiates elution of NO from said device. Furthermore, a manufacturing method for said device is disclosed.

An Ni-Fe alloy material suitable for forming a ferromagnetic Ni-Fe alloy thin film is provided. The magnetic thin film produces a small number of particles during sputtering, and excels in corrosion resistance and magnetic properties. A method of manufacturing an Ni-Fe alloy sputtering target used to make the thin film is also provided. In addition, an Ni-Fe alloy sputtering target for forming magnetic thin films is provided. The sputtering target is characterized in that it has: an oxygen content of 50 ppm or less; an S content of 10 ppm or less; a carbon content of 50 ppm or less, and a total content of metal impurities other than the alloy components of 50 ppm or less. Such an Ni-Fe alloy target can be produced by melting and alloying high-purity materials obtained by dissolving the raw materials in hydrochloric acid, and performing ion exchange, activated-charcoal treatment, and electrolytic refining.

The invention discloses a method and a device for detecting and selecting laser-induce enhanced Raman spectrum in a liquid. The method comprises the following steps of: focusing the inducing light on a suspending to-be-detected sample and forming a three-dimensional light well in liquid; enriching metal nanoparticles on the surface of the to-be-detected sample; and coinciding the focuses of exciting light and inducing light, thereby forming a laser-induce enhanced Raman signal. The realizing method disclosed by the invention is simple and convenient, is obvious in spectrum enhancing effect and is fit for quick detection for in-situ Raman spectrum of a biological sample under a liquid environment; the to-be-detected sample and the metal nanoparticles are hatched together; the method is especially fit for the field of quick detection for diseases, epidemic situation, food safety, and the like; the toxic damage to the biological sample caused by the metal nanoparticles is greatly reduced, so that the true physiological status of living cell can be extremely reflected; the optical tweezers formed according to the method provided by the invention can be used for sorting, extracting and eluting, can realize the function of identifying and sorting Raman spectrum of a single living cell, and is beneficial to the follow-up study on the biological sample.

The invention discloses a production method of a crosslinked chitosan microsphere heavy metal ion adsorbent. The method comprises the following steps of: (1) protecting amino on chitosan C2 with formaldehyde by taking chitosan as a material and taking liquid paraffin as an organic dispersion medium, and preparing a crosslinked chitosan microsphere with a reversed phase suspension crosslinking method; and (2) chemically grafting the crosslinked chitosan microspheres by taking the crosslinked chitosan microsphere as a parent body and taking ethylene diamine as a ligand to obtain the crosslinked chitosan microsphere heavy metal ion adsorbent. The crosslinked chitosan microsphere heavy metal ion adsorbent produced with the method has high selective adsorption on Cu<2+> ions.

Provided is a vascular stent used in percutaneous coronary intervention (PCI) which is specially designed for multiple drug loading and improved drug elution, including: a plurality of ring structures extending in the longitudinal direction of the vascular stent, including a plurality of struts disposed in a zigzag formation and connected to each other to form a cylindrical loop; and a plurality of link structures disposed between the ring structures and connecting the ring structures in the longitudinal direction of the vascular stent, wherein each of the struts in a ring structure is connected to adjacent struts in the same ring structure through one of a plurality of linking ends, a slot loaded with drugs is formed in the strut in the longitudinal direction of the strut, and a multi-layer structure comprising a plurality of layers of drugs is loaded in the slot. The vascular stent effectively inhibits restenosis by loading a large amount of a drug or various types of drugs in multiple layers in slots of struts in the vascular stent and controlling elution of the drugs, and can be easily installed in a serpentine coronary artery with excellent flexibility. In addition, the link structure of the vascular stent is disposed in a symmetric formation, and thus, side branch accessibility is excellent and an additional coronary intervention can be performed easily in a branched artery.

The invention discloses a method for recovering scandium in waste acidic water from titanium dioxide production, which is simple in washing liquid preparation and convenient to use. The method is characterized by comprising the following steps of extraction, washing, back extraction, acidic dissolution, oxalic acid precipitation and scorching. The method is simple in process and convenient to operate, dilute sulphuric acid and hydrogen peroxide are respectively added in a cascade washing process, so that the washing liquid is maintained at a certain temperature to facilitate titanium elution in the washing process, and the hydrogen peroxide is prevented from being decomposed massively at high temperature in an acidic medium, meanwhile, in several stages after feeding of hydrogen peroxide, an organic phase washed with hydrogen peroxide is further washed with pure sulphuric acid, and free hydrogen peroxide is washed for recycle so as to reduce the loss of hydrogen peroxide; and in addition, in the back extraction process, a back extraction solution is retained in a reaction kettle for repeated back extraction, so that the frequency of filter pressing is reduced, a process is simplified and the loss of Sc is reduced.

The blood pump according to the present invention comprises a casing having a blood inlet and a blood outlet, and an impeller for circulating blood by rotating inside the casing, wherein both the casing's and the impeller's surfaces in contact with blood are formed of a biocompatible metal, and onto the surfaces in contact with blood a coating film of a hemocompatible material comprising a phospholipid polymer is formed. According to the present invention, the blood pump effectively suppresses the development of thrombi without activating blood coagulation factors such as thrombocites (blood platelets) in the blood, thanks to a coating film of a hemocompatible material made of a phospholipid polymer being formed onto the surfaces in contact with blood of the casing and the impeller composing the main part of the blood pump.

The invention provides a preparation method of peony general glycoside, which comprises the following steps: soaking radix paeoniae alba or red paeonia in 2-10 times (weight) of hydrophilic solvent, wherein the hydrophilic solvent is water or a water solution containing less than 30% of methanol, ethanol, propanol and isopropanol; after soaking, percolating at low temperature of 5-40 DEG C; and concentrating the percolate, filtering to obtain the extracting solution, and passing the extracting solution through macroporous resin. The transfer rate of penoniflorin and peony Albiflorin is 85-90%, and the purity of the peony general glycoside extract is high.

A device and method for extracting micro-plastic in settlings are provided. The device includes a filter drum, a liquid storage tank, a motor, a pedestal, a screen sieve and a transmission shaft. The filter drum is mounted in the liquid storage tank. The upper port of the filter drum is provided with the screen sieve. The liquid storage tank and the motor are mounted on the pedestal. The bottom of the liquid storage tank is provided with a drainage hole communicated with the filter drum. The motor is connected to one end of the transmission shaft through a rotating shaft, and the other end of the transmission shaft passes through the drainage hole and is connected to the bottom of the filter drum. The device and the method are advantageous in that (1) dispersion effects of muddy settlings and elution effects of a sample are obviously improved; (2) the recovery rate of the sample is increased, (3) the extraction efficiency is greatly increased, (4) extraction time is shortened and (5) labor is saved.

The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.

The invention relates to a preparation method of a modified magnetic chitosan microsphere heavy metal ion adsorbent, and in particular relates to a modified magnetic chitosan material which has good selectivity and adsorptive property for heavy metal ions in food, and can be recycled, and a preparation method of the modified magnetic chitosan material. Specifically, the invention discloses the preparation method of the modified magnetic chitosan microsphere heavy metal ion adsorbent. The method comprises the steps of preparing magnetic chitosan microspheres by taking chitosan powder as a raw material, further preparing the magnetic chitosan microspheres into hydroxypropyl chlorine magnetic chitosan microspheres, and carrying out chemical grafting on the hydroxypropyl chlorine magnetic chitosan microspheres by taking the hydroxypropyl chlorine magnetic chitosan microspheres as parents and 2-aminothiazole as ligands to obtain the modified magnetic chitosan microsphere heavy metal ion adsorbent. The modified magnetic chitosan microsphere heavy metal ion adsorbent prepared by the method has preferential adsorption for Hg2+ ions.

The invention discloses a method for preparing a crosslinked chitosan microsphere heavy metal ion adsorbent. The method comprises the following steps of: 1) by taking chitosan as a material and liquid paraffin as an organic dispersing medium, protecting amino on chitosan C2 by using formaldehyde, and by taking epoxy chloropropane as a crosslinking agent, preparing a crosslinked chitosan microsphere by a reversed phase suspension crosslinking method; and 2) by taking the crosslinked chitosan microsphere as a matrix and 2-(chloromethyl)benzimidazole as a ligand, chemically grafting the crosslinked chitosan microsphere to obtain the crosslinked chitosan microsphere heavy metal ion adsorbent. The crosslinked chitosan microsphere heavy metal ion adsorbent prepared by the method has high adsorption speed, can selectively adsorb the Hg<2+> ion highly, can easily and completely desorb ions and can be repeatedly used.

The invention discloses a preparation method and an application technology of a dimethyl diallyl ammonium chloride modified towel gourd vegetable sponge adsorbent, and is characterized in that the preparation method comprises the steps: peeling and denucleating mature fruits of towel gourd, washing with water to remove soil and impurities, drying, crushing, and carrying out chemical treatment drying to obtain acylating-chlorination towel gourd vegetable sponge; adding, by the mass percentage, 56-71% of dimethyl diallyl ammonium chloride, 10-20% of the acylating-chlorination towel gourd vegetable sponge, 12-26% of water and 0.5-2.0% of ammonium persulfate into a reactor, wherein the sum of the percentages of all the components is 100%; under the nitrogen protection and at a constant temperature of 45-55 DEG C, stirring and carrying out a reflux reaction for 4-5 h; and after cooling, washing with deionized water, carrying out suction filtration until a filtrate is neutral, drying in a vacuum drying oven, and thus obtaining the dimethyl diallyl ammonium chloride modified towel gourd vegetable sponge adsorbent. The adsorbent has the advantages of quite high adsorption capacity on grease, excellent physicochemical and mechanical properties, strong regeneration capacity, capacity of being repeatedly used many times, low cost, greenness and environmental protection.

The invention discloses a preparation method of undecylene amido propyl betaine modified diatomite. The method is characterized in that after diatomite is treated with 2 mol / L HCL, pretreated diatomite is obtained; the pretreated diatomite is subjected to organic modification by using absolute ethyl alcohol and (3-chloropropyl)trimethoxysilane; then 45%-55% of deionized water and 8%-18% of undecylene amido propyl betaine are added to a reactor in percentage by mass, the mixture is stirred for dissolution, then 28%-38% of organic diatomite is added, the mixture is evenly mixed, 5%-8% of hydrogen peroxide with the mass percentage concentration of 30% is added, the sum of percentages of all components is 100%, the mixture is stirred at the constant temperature of 65 plus / minus 2 DEG C for a reflux reaction for 4-6 h, solid-liquid separation is performed after cooling, separated solids are washed with deionized water until the filtrate is neutral, filtration and drying are performed, and the undecylene amido propyl betaine modified diatomite is obtained. The adsorbent has very high adsorption capacity for oil, has excellent physical and mechanical properties and high regeneration capacity, and capable of being reused, low in cost and environment-friendly.

The invention discloses a preparation method and an application of polar group-modified pendent double bond post-crosslinked polydivinylbenzene resin. The preparation method comprises: firstly performing a polymerization reaction of divinylbenzene monomers, polar monomers, and a pore forming agent to obtain a polar group-modified pendent double bond post-crosslinked polydivinylbenzene resin precursor; performing further crosslinking through a Friedel-Crafts reaction to obtain the polar group-modified pendent double bond post-crosslinked polydivinylbenzene resin. The preparation method is simple, and low in cost, and the prepared post-crosslinked resin has a high specific surface area and a special pore structure; the post-crosslinked resin can be used for adsorbing and removing organic matter in waste water, has the effect of selective adsorption, and especially has obvious selective adsorption effect on Congo red and / or rhodamine B.