307results about How to "Small dead volume" patented technology

The present invention provides for a novel system of extracting and purifying nucleic acids (DNA, RNA, etc.) from cellular material like blood. Such a system of extraction and purification relies on novel monolithic microfluidic devices and methods of using these devices. Such devices comprise numerous components, monolithically-incorporated on an single chip, and further comprising novel nucleic acid binding materials. The present invention is also directed to method of preparing such novel nucleic binding materials.

A piston assembly (12) comprising a reciprocating sleeve (14) incorporating an integral internal piston surface (28), which sleeve is slidably mounted upon a cylinder head (18) so as to define a piston chamber (26) therewith, the piston chamber being sealed in the vicinity of the cylinder head (18) by a circumferential static seal (20) that acts to seal against the reciprocating sleeve (14). The static seal (20) may occupy a horizontal plane and may include sacrificial wear zones and be formed from a graphite-based material. The piston assembly may be an oversquare assembly for use in an oil-free environment for processing high temperature gases, for example, a hot gas engine or heat pump or heat engine such as may be used in an energy storage system.

Embodiments of the invention provide devices, methods and systems for performing microfluidic chromatography. Particular embodiments provide microfluidic chromatography column devices which can perform chemical separation using small sample volumes and low pressure differentials across the column. One embodiment provides a microfluidic chromatography column device comprising a first, second and third capillary tube. A chromatographic packing is disposed in the second tube with a first and second support layer disposed on opposite ends of the second tube. The support layers are disposed in a substantially flat orientation within the tube. An external coupling joins the tubes such that the tubes are fluidically sealed. The device is configured to have a fluidic resistance such that a pressure differential across the column of less than about 10 psi produces a flow rate through the device of at least about 0.5 ml / min for a liquid solution.

An analyte pre-concentrator for gas chromatography is disclosed generally comprising a tube having a restricted outlet and packed with an adsorbent, wherein the tube serves as the liner of a chromatographic injector, as an adsorbent trap coupled to a chromatographic column, and / or as an adsorbent trap coupled to a headspace sampler. Preferably, a heating device allows the tube to be heated. In a preferred embodiment, the analyte pre-concentrator further comprises a column isolating accessory so that a chromatographic column can be temporarily isolated from substances flowing through the tube.

The invention discloses a micro bio-chip for real-time detection of substances in body fluids, the micro bio-chip is assembled by a circulating microfluidic chip and a film biosensor, parts of the circulating microfluidic chip in turn are connected with each other to form a complete fluid pathway, and the film biosensor includes a working electrode, a counter electrode and a reference electrode; in a testing room of the circulating microfluidic chip, one or more film biosensors are embedded in the manner of series connection, or parallel connection / series connection and parallel connection, so that the surfaces of the embedded film biosensors are always contacted with the body fluid flowing by for real-time detection of the substances in the body fluids.

A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured to adjust a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

Shutoff valve apparatus comprising: a valve block 80 having a valve chest on which gas collects temporarily, an inlet flow path 34 which makes gas flow into a valve chest, an outlet flow path 40 into which gas is made to flow out of a valve chest, a valve seat 44 located in the inlet port of an inlet flow path, or the outlet port, a diaphragm valve element 38 located in the opposed position of the valve seat and closes the valve port by contacting with the valve seat, pressing means 82 for pressing diaphragm valve element, an actuator plate 84 disposed at the opposite side of the valve chest so that the diaphragm valve element may be opposed, an operation chamber 86 divided with the actuator plate, and an operation gas supply exhaust mechanism in which operation gas is made to supply or exhaust into the operation chamber.

The invention belongs to the field of oil gas and oil well development, discloses a system for sampling and monitoring components of shaft fluid of a gas field and particularly relates to a system for sampling and monitoring components of the shaft fluid of the gas field and a sampling and analysis method thereof, which are designed aiming at environmental characteristics of a H2S / CO2-containing gas field. A sampling process of a sampler of a gas well is safer, an experimental process is more accurate and quicker, and the application pressure range is wider and safer, and an experimental result of researching the change of high-content hydrogen sulfide gas components is more accurate; and a device is safe and reliable. Through the system and the method, the problems that fluid pressure cannot be obtained and fluid deposit in the shaft cannot be predicted because the measurement cannot be performed in the gas field containing high content of H2S / CO2 can be solved and theoretical basis is provided for a gas well capacity design, production parameter optimization and the formulation of technical measures.

The bottom of the cuvette comprises a curved raceway placed so as to guide the oscillating movement of a ball inserted into the cuvette. In addition, the cuvette comprises means of attachment, in two perpendicular directions, to adjacent unit cuvettes. The cuvettes can thus be stored as plates in a feed magazine of an analytical device. The analytical device comprises several stations placed around a rotary ring. Only where it is desired to determine the clotting time of the blood contained in the cuvette a ball is introduced into the latter, at a ball distribution post. The cuvette equipped in this way is then brought to a station where the test is carried out. The major advantage of the invention is the polyvalence of the cuvette and of the analytical device.

The invention provides a long rock-core holder comprising a holder cylinder (1) and a high-pressure-resistant rubber sleeve cylinder (2) sleeved in the holder cylinder (1). The high-pressure-resistant rubber sleeve cylinder (2) comprises a cylinder wall (21), an inner cavity (23) positioned in the cylinder wall (21), and cylinder ports positioned on two ends of the inner cavity (23). The inner cavity (23) is in a shape of a straight cylinder. The inner diameter at the cylinder ports on the two ends equals the inner cylinder at the middle of the inner cavity (23). With the holder provided by the invention, long rock core axial multi-oressure-measuring-point detection can be carried out. The method has the advantages of low end face effect, small dead volume, high static confining pressure, and low measurement error.

The invention relates to a high-pressure chamber for preferably atomizing or injecting metered amounts of fluid, such as pharmaceutical preparations. The components of the high-pressure chamber determining the strength thereof are made of a sintered metal body having plastic regions directly attached thereto. The plastic regions allow corrosion resistance and material compatibility of the interior of the high-pressure chamber and fluids placed under pressure therein. The shape of the sintered metal body provides the entire system with the rigidity required for high-pressure operation and with directly molded connection elements prescribing the type of connection to other components. The components of the high-pressure chamber determining the strength thereof are thereby deformed or crimped to each other, so that inexpensive and quick overall assembly processes are available.

A hollow fiber membrane type artificial lung comprising a hollow fiber bundle composed of multiple porous hollow fiber membranes (8) arranged in one direction; a heat exchange pipe bundle composed of multiple heat exchange pipes (9) arranged in one direction across the hollow fiber membranes and piled one upon another, the heat exchange pipe bundle disposed in parallel to the hollow fiber bundle; and potting materials (10) charged over regions including both end portions of the hollow fiber membranes and heat exchange pipes, which potting materials define a blood flow channel extending across the hollow fiber membranes and heat exchange pipes. Further, there is provided a housing comprising blood ports (3a,3b) facing both ends of the blood flow channel; gas headers (4a,4b) facing both ends of the hollow fiber membranes and providing gas ports (5a,5b); and heat exchange headers (6a,6b) facing both ends of the heat exchange pipe bundle and providing heat exchange liquid ports (7a,7b). Blood is caused to flow through the blood flow channel, and an oxygen-containing gas is passed through the cavity of the hollow fiber membranes to thereby carry out gas exchange. A heat exchange liquid is passed through the cavity of the heat exchange pipes to thereby carry out heat exchange. There can be provided a hollow fiber membrane type artificial lung having a heat exchange part annexed to a gas exchange part without any superfluous increase of charged blood quantity.

A pump arrangement includes a pump having a pump inlet and a pump outlet, and a safety valve arranged between the pump outlet and an outlet of the pump arrangement and having a valve seat and a valve lid. The valve seat, the pump outlet and the pump inlet are patterned in a first surface of a first integrated part of the pump arrangement, whereas the valve lid is formed in a second integrated part of the pump arrangement. An inlet of the pump arrangement and a fluid region fluidically connected thereto are formed in a third part of the pump arrangement. The second integrated part is arranged between the first integrated part and the third part of the pump arrangement such that a pressure in the fluid region has a closing effect on the safety valve, the pump inlet and the inlet of the pump arrangement being connected fluidically.

The bottom of the cuvette comprises a curved raceway placed so as to guide the oscillating movement of a ball inserted into the cuvette. In addition, the cuvette comprises means of attachment, in two perpendicular directions, to adjacent unit cuvettes. The cuvettes can thus be stored as plates in a feed magazine of an analytical device. The analytical device comprises several stations placed around a rotary ring. Only where it is desired to determine the clotting time of the blood contained in the cuvette a ball is introduced into the latter, at a ball distribution post. The cuvette equipped in this way is then brought to a station where the test is carried out. The major advantage of the invention is the polyvalence of the cuvette and of the analytical device.

The invention belongs to instrument technical field, in particular to a thermal expansion continuous micro-flow high-pressure gradient pump of high-effect liquid spectrometer, composed of a pressure-resistance heating pump, a micro-flow pump control system, a switch valve, a pressure sensor, and a liquid bottle connected in turn. The invention uses the theory of liquid thermal expansion, via pre / real-time calculation to control the heating process of heated liquid, to obtain micro-flow at 10-5 to 10-9L / min level via increasing temperature, as constant flow, linear gradient liquid flow and other types, and the output pressure can reach 10-35MPa. And the invention uses a valve switch system to realize automatic alternative operation of two pump groups, to confirm continuous output of micro flow, and realize at least binary flow equal / gradient watch, and uses dual-pressure balance point exchange technique, to confirm stable flow speed and stable pressure in pump switch.

The invention belongs to the technical field of pretreatment of an analytical chemistry experiment, and relates to a pressurization solid phase extraction device for sample pretreatment. The device comprises two parts including a pipetting assembly and an extraction and elution component, wherein the pipetting assembly is formed by a plurality of arranged pipetting channels; each pipetting channel comprises a sample cup, an injection pump, a first one-way valve, a second one-way valve, a movable connecting head, a first teflon tube and a second teflon tube; the extraction and elution component comprises column bolts, solid-phase extraction columns and a collector; the number of the column bolts corresponds to that of the movable connecting heads; all the column bolts correspond to the solid-phase extraction columns one by one, and can be respectively inserted into the solid-phase extraction columns; each column bolt is provided with a flow through hole, and medium can flow into the corresponding solid-phase extraction column through the flow through hole; the collector is arranged under the solid-phase extraction columns and is used for collecting the medium discharged by the solid-phase extraction columns. The pressurization solid-phase extraction device can provide stable and accurate flow speed, is capable of treating a great deal of samples once, can basically realize complete sample introduction, and can be used for treating the plurality of samples at the same time.

A patient interface assembly includes a housing that defines an inlet port and an outlet port. A jet pump receives pressurized gas flow from the inlet port and delivers the gas flow to the outlet port. A nebulizer is fluidly coupled to the outlet port and positioned to introduce medication into the gas flow and deliver medicated gas flow to a patient.

A complex fluid machine has an expansion-compressor device, a pump, and a motor generator, wherein the expansion-compressor device, the pump, and the motor generator are operatively connected and arranged in series, and a power transmitting device for disconnecting the pump from the motor generator, when the expansion-compressor device is driven by the motor generator so as to be operated as a compressor device.

The present invention relates to a method for industrial separation and purification of lactoferrin from beestings. It is characterized by that it uses beestings defatted serum as raw material, adopts enzyme method to remove casein and utilizes combination of membrane separation technique and industrial chromatographic technique to separate out lactoferrin whose purity is above 90% from beestings.

An analyte pre-concentrator for gas chromatography is disclosed generally comprising a tube having a restricted outlet and packed with an adsorbent, wherein the tube serves as the liner of a chromatographic injector, as an adsorbent trap coupled to a chromatographic column, and / or as an adsorbent trap coupled to a headspace sampler. Preferably, a heating device allows the tube to be heated. In a preferred embodiment, the analyte pre-concentrator further comprises a column isolating accessory so that a chromatographic column can be temporarily isolated from substances flowing through the tube.

A microfluidic system includes a plate-shaped microfluidic chip having a flow path, a liquid introduction tube for supplying liquid to the flow path, the liquid introduction tube having an end communicating with an end of the flow path and its other end that can be soaked in the liquid that is to be supplied to the flow path, and a liquid discharge head communicating with the other end of the flow path for discharging liquid that has passed through the flow path.

A spraying device such as an atomizer mounted to a painting robot of an installation for the serial spraying of work pieces contains both the color change valve arrangement and a metering pump located between the color changer and the spray head. The metering pump is preferably a valveless rotary piston pump, and for the color changer a suitably miniaturized arrangement and construction for the valve assemblies is preferred.

The invention belongs to the field of development of oil and gas wells, and relates to a wellhead sampling device, in particular to a wellhead sampler designed for the characteristic of gas field environment containing high content of H2S / CO2, and a sampling system thereof. The sampling device comprises a sampling valve, a sampling bottle and a pressure reducing valve, wherein two ends of the sampling bottle are in threaded connection with the sampling valve; the external connection of the sampling valve is external-thread clamping sleeve type connection; the pressure reducing valve is used for carrying out pressure reduction and sample injection on high-pressure gas in the sampling bottle; the pressure reducing valve comprises an outer conveying sample injection port which is connected with an outer detector; and the outer conveying sample injection port is a clamping sleeve type connection port so as to eliminate dead angles of pipelines. In the invention, the field sampling for analysis is achieved, the result of experimental analysis has good consistency, the device is safe and reliable, and the corrosion resistance to H2S / CO2 is strong. In the gas field sampler disclosed by the invention, the sampling process is safer, the experimental process is more precise and faster, the applicable pressure range is wider, and more safety is achieved.

A device for measuring the volume flow or other substance properties of a gas, whose direction of flow can reverse. The arrangement contains a specially designed Y-piece, which is used for branching into direction-dependent flow paths and can be used at the same time for the measurement. Such devices can be preferably used in the area of mechanical respiration.

The invention discloses an automatic sampling gun, relating a sampling device, aiming at providing an automatic sampling gun which has small volume and high degree of automation and is suitable for making up multi-channel sample devices. The antomatic sampling gun comprises a unit upper mounting rod and a unit lower mounting rod, wherein, the inner surfaces corresponding to the unit upper mounting rod and the unit lower mounting rod are respectively fixedly provided with an upper guide rail and a lower guide rail,the top of the unit upper mounting rod is provided with an electric motor, the output shaft of the electric motor is provided with a driving synchro wheel, the lower end of the unit upper mounting rod is provided with a driven synchro wheel,a synchro belt is installed onto the driving synchro wheel and the driven synchro wheel, the top of the unit lower mounting rod is fixedly connected with an upper connecting block, the upper connecting block is provided with an upper slider, the upper slider is arranged on the upper guide rail, above the driven synchro wheel is fixedly provided with a lower connecting block, the lower connecting block is provided with a lower slider, the lower slider is arranged on the lower guide rail, and the synchro belt is fixed with the upper connecting block.

The co-using interface of the method of atom fluorescence spectrum and generation of the hybride in capillary electrophoresis relates to an apparatus thereof. This interface provides the key solutios that the capillary electrophoresis needs stable electric circuit, and that the method of atom fluorescence spectrum needs to convert analysed agent into hydride. The interface includes cross passage 1, T passage with small inner diameter 5, T passage with large inner diameter 7 and gas-liquid separator 6, cross passage 1 connected in turn to T passage with small inner diameter 5, T passage with large inner diameter 7 and gas-liquid separator 6. The interface has ingenious and novel design, having such features as small dead volume, short connecting piping and simple and practical operation.

The invention relates to a large-volume leather bag type constant-pressure energy accumulator and application thereof. The large-volume leather bag type constant-pressure energy accumulator comprisesa shell and further comprises a leather bag, a variable area piston, a floating piston, a piston and a flange which are arranged in the shell. The floating piston sleeves a piston rod of the variablearea piston. The bottom end of the piston rod of the variable area piston is connected with the piston, and a through hole is formed in the center axes of the variable area piston and the piston, an inflation valve is connected between the through hole and the leather bag, the bottom of the through hole is connected with a cover plate, the piston is provided with a one-way valve I and a one-way valve II, and the flange is connected with the inner wall of the bottom of the shell. The novel large-volume leather bag type constant-pressure energy accumulator has the advantages that leakage is notlikely to happen, the service life is long, inertia is small, the response is sensitive, and the applicable volume range is wide; and the novel large-volume leather bag type constant-pressure energy accumulator can be widely applicable to various hydraulic systems.

A preparative circulating chromatography apparatus can be applied in chromatographic separation preparation of target components in a complex sample and is mainly advantaged in that: (1) with pre-treatment and concentration of an on-line sample, large-volume sampling on the premise of a high column efficiency can be achieved; (2) through position exchange between a separation column and a trapping column, circulating separation and purification of the target components are achieved and the preparative circulating chromatography apparatus is less in circulating dead volume and column efficiency loss; and (3) through concentration-elution of a finish product through the trapping column, a separation-concentration integrated process is achieved, wherein the three functions are achieved through adjustment of an adsorption performance of the trapping column and valve exchange. Compared with a conventional preparative chromatography method, the apparatus has significant advantages on separations of natural products, drugs and chemical products, especially on separation and preparation of micro-scale targeted components and unstable components in a mixture.

The invention relates to an ultrasonic atomization atmospheric-pressure glow-discharge ionization apparatus. The apparatus comprises an ultrasonic atomization apparatus, an atmospheric-pressure glow-discharge apparatus, a high-voltage power supply and a sample inlet interface, wherein the ultrasonic atomization apparatus comprises a sample inlet pipe, a sample container, a liquid inlet pump, an ultrasonic atomizer and a spraying nozzle; the sample inlet pipe is connected with the sample container and the liquid inlet pump; the liquid inlet pump is used for extracting a water sample of a liquid sample; the liquid outlet end of the liquid inlet pump is connected with the ultrasonic atomizer; and the atmospheric-pressure glow-discharge apparatus comprises a discharging needle, a high-voltage glow reaction chamber and a sample inlet conical opening. By virtue of ultrasonic atomization, the liquid sample, which is not suitable for volatilization, can be directly sampled, without requiring complex pretreatment, so that sensitive and rapid mass spectrometry can be realize; and therefore, the ultrasonic atomization atmospheric-pressure glow-discharge ionization apparatus is wide in application prospect in rapid mass spectrometry of food, pesticides, environment and the like.