40 results about "Stable isotope ratio" patented technology

The invention belongs to the technical field of environment monitoring, and particularly relates to a quantitative recognition method for contribution of autogenous source organic carbon of a surface water body. Based on the stable isotope ratio and the three-dimensional fluorescence spectrum, the water DOM source analysis method is constructed, the source and characteristics of DOM are comprehensively analyzed, and the characteristic information and contribution of autogenous source organic carbon are specifically recognized. The three-dimensional fluorescence spectrum has the advantages of being rapid in detection, small in sample use amount and the like, DOM sources can be rapidly and qualitatively identified, different DOM sources can be quantitatively calculated through stable isotope ratio analysis, and meanwhile the research area land utilization, hydrological water environment and water quality parameters are combined, so that the DOM sources can be rapidly and qualitatively identified. And the source and migration rule of the DOM in the water body can be determined more comprehensively. The quantitative identification method provided by the invention has the advantages of more comprehensive water DOM research, more complete identification system and more reliable traceability result.

The invention discloses a device and a detection method for online oxidation-enrichment-reduction determination of nanogram-level nitrogen isotopes in a solid, and belongs to the technical field of nitrogen isotope analysis. The device comprises an oxidation system, a nitrogen oxide enrichment system, a nitrogen reduction system, a mass spectrum interface system, a stable isotope ratio mass spectrometer and pipelines for connecting all parts of the device. The device provided by the invention greatly reduces the quantitation limit of the nitrogen isotope in the solid sample, can realize high-precision determination of the nanogram-level nitrogen isotope in the solid sample, and has the advantages of simplicity and convenience in operation, high accuracy and the like.

The invention provides a detection method of a monomer nitrogen-hydrogen-chlorine multidimensional stable isotope of chloroaniline and a monitoring method of chloroaniline, and relates to the technical field of isotope detection. According to the method, a gas phase-double oxidation tube-stable isotope ratio mass spectrum is adopted to detect and calculate a nitrogen stable isotope ratio in chloroaniline, and a gas phase-chromium filler cracking tube-stable isotope ratio mass spectrum is adopted to detect and calculate a hydrogen stable isotope ratio; the gas phase-multi-collector inductively coupled plasma mass spectrometry is adopted to detect and calculate the chlorine stable isotope ratio, so that the determination of the multi-element stable isotope ratio of nitrogen, hydrogen and chlorine in o-chloroaniline, m-chloroaniline and parachloroaniline is realized, the detection degree is more comprehensive, and the detection result is accurate, efficient and reliable. The range of each isotope signal value in the detection process is controlled, and high accuracy of the detection result can be ensured. The detection method provided by the invention provides a new method and means for deep research and monitoring of chloroaniline traceability, degradation mechanism and environmental homing.

The present invention provides a pretreatment method for determining the stable isotope ratio of non-exchangeable hydrogen in acetic acid, belonging to the field of stable isotope analysis of foods. The pretreatment method comprises the following steps: adding a reaction reagent to a vinegar sample extract to replace the carboxyl hydrogen in the acetic acid with the exchangeable hydrogen to achieve isotope exchange equilibrium. The present invention can be used to simply and rapidly determine the isotope ratio of non-exchangeable hydrogen in acetic acid in vinegar products and can be used in research and applications for detecting the authenticity or adulteration of vinegar products.

The invention provides a method for analyzing the oxygen stable isotope ratio of water in a highly alcoholic beverage, and relates to the technical field of oxygen isotope analysis. The analysis method comprises the following steps: selecting at least two water samples with known oxygen isotope ratios and different values as additive water; diluting the same high-alcohol beverage to be detected by adding water to obtain a diluted sample; measuring an oxygen isotope ratio; and according to the oxygen isotope ratio of the added water and the oxygen isotope ratio of the diluted sample, calculating the oxygen isotope ratio of the water in the highly alcoholic beverage to be measured. According to the analysis method, the concentration of ethanol is reduced through dilution, so that interference of isobaric elements is avoided; and physical separation is replaced by mass conservation equation calculation, and a complex distillation device is not needed. According to the method, pretreatment is remarkably simplified, cost and errors are reduced, and rapid and accurate analysis of the water oxygen isotope in the highly alcoholic beverage is achieved.

The application discloses a method and a system for identifying exogenous plant protein adulteration in pure dairy products, which comprises the following steps: simultaneously extracting milk protein and lactose from the pure dairy products to be tested; using an elemental analyzer-stable isotope ratio mass spectrometer combined system to determine the carbon stable isotope ratio of the milk protein and the lactose; calculating the carbon isotope characteristic difference; making a qualitative determination of adulteration based on the statistical threshold established for pure cow milk; and quantitatively calculating the adulteration proportion of the exogenous plant protein by using a linear regression model constructed in advance. The system comprises a sample pretreatment module, an isotope detection module, a data processing module, a determination module and a quantitative calculation module. The application uses isoelectric point precipitation combined with organic solvent extraction to extract milk protein, and uses ethanol low-temperature recrystallization to purify lactose, and introduces lactose as an endogenous isotope internal standard, which effectively offsets the system error, can quickly determine the adulteration behavior and accurately calculate the adulteration proportion of the exogenous plant protein.

The present invention provides a method for determining the isotope ratio of non-exchangeable hydrogen in ethanol in wine. The method comprises the following steps: 1) determining the δD value of water in the wine; 2) adding water with a lower δD value than the water in the wine to prepare a wine solution; 3) taking an ethanol standard and diluting the ethanol standard with water having the same or similar δD value as the wine solution to the same alcohol content as the wine solution; and 4) determining the δD ratio of stable hydrogen ethanol in the wine sample solution and the standard solution using gas chromatography-pyrolysis-stable isotope ratio mass spectrometry. 测 5) A standard curve is constructed based on the given value of non-exchangeable hydrogen in the ethanol standard and the measured hydrogen isotope values, and the isotope ratio of the non-exchangeable hydrogen in the ethanol in the wine sample is calibrated and obtained. This method can be used to simply and rapidly determine the isotope ratio of the non-exchangeable hydrogen in the ethanol in wine and can be used in research and applications for wine authenticity and adulteration detection.

The invention provides a medicine raw material homology judgment method and device, computer equipment and a medium, and belongs to the field of medicine identification, and the method comprises the following steps: obtaining a plurality of same sample medicines from different sources; determining the stable isotope abundance values of carbon, nitrogen, oxygen and hydrogen in the drug sample through a stable isotope ratio mass spectrometry and a chromium-assisted stable isotope ratio mass spectrometry; taking the stable isotope abundance value as a variable, and classifying sample drug sources by adopting a hierarchical clustering analysis and principal component analysis method to obtain a plurality of drug sources; obtaining stable isotope abundance values of to-be-classified target drugs, and respectively determining the stable isotope abundance values of the target drugs and Z values of the stable isotope abundance values of all classes based on a Z value analysis method; under the condition that the Z values of the stable isotope abundance values of all the elements are smaller than or equal to the preset threshold value, it is determined that the target drug and the sample drug of the corresponding category are homologous drugs, and therefore the accuracy of drug detection is improved.

The present application relates to a method for identifying different seasons of tea, and belongs to the technical field of tea quality detection. The existing tea season identification relies on sensory or single component analysis, which is highly subjective and lacks accuracy. The present application collects tea samples, detects the δ 15 N stable isotope ratio and the contents of EGC, EGCG, ECG and CAF organic components in the tea samples, and substitutes these data into the linear discriminant models for spring, summer and autumn respectively to calculate the values of Y 春季 , Y 夏季 and Y 秋季 . The season corresponding to the largest value is the season to which the tea sample belongs. This method uses multiple indexes in combination with linear discriminant models to objectively identify the season of tea, and can be used in tea quality control, market supervision and tea traceability, etc., providing a scientific basis for accurate judgment of the season of tea.

The invention discloses a tea tree variety cold resistance early identification method, device, equipment, medium and product, and relates to the field of tea tree variety stress resistance identification. The method comprises the following steps: acquiring tea samples of different cold-resistant tea tree varieties in four seasons; determining a stable isotope ratio in each tea sample; a partial least squares discriminant analysis method is adopted, the stable isotope ratio in each tea sample in four seasons of the year is used as an independent variable, the cold resistance of the tea tree variety corresponding to the tea sample is used as a dependent variable, and a cold resistance quantitative prediction model is established; obtaining tea samples of the to-be-detected tea tree variety in four seasons, and determining stable isotope ratios of the to-be-detected tea tree variety in the tea samples in all seasons; and inputting the stable isotope ratio of the to-be-tested tea tree variety in the tea samples all the year round into the cold resistance quantitative prediction model to obtain the cold resistance of the to-be-tested tea tree variety. Early identification of the cold resistance of the tea tree variety can be realized.

According to various aspects of the present disclosure, a method is provided for the traceability and source identification of cosmetic-grade ethanol. The method includes the step of measuring at least two stable isotope ratios.

The present invention proposes a method for determining the stable isotope ratio of non-exchangeable hydrogen sites of glycerol in edible oils and fats, belonging to the field of stable isotope analysis technology. The method comprises the following steps: a) preparing a preliminary glycerol sample; b) determining the stable hydrogen isotope ratio of glycerol in the preliminary glycerol sample using a gas chromatography-pyrolysis-stable isotope ratio mass spectrometer; c) selecting glycerol with a known stable isotope ratio of non-exchangeable hydrogen sites as a working standard substance, processing according to step a), and determining according to step b) to obtain the stable hydrogen isotope ratio of the glycerol working standard substance; and calculating the stable isotope ratio of the non-exchangeable hydrogen sites of glycerol in the edible oil to be tested by comparing the measurement result of the stable hydrogen isotope ratio of the glycerol working standard substance with the known stable isotope ratio of the non-exchangeable hydrogen sites of the glycerol working standard substance. The method is rapid and accurate, providing a new analytical method for adulteration detection and authenticity control of oils and fats.

This invention discloses a method for determining the origin of tea based on the stable isotope ratio of EGCG monomers, belonging to the field of tea origin identification technology. It aims to solve the technical problem of insufficient accuracy in existing tea origin identification methods. The method involves measuring the overall stable isotope ratio δ in the tea sample. 13 C 茶叶 δ 15 N 茶叶 δ 2 H 茶叶 δ 18 O 茶叶 and the δ¹² value of stable isotope ratios of EGCG monomers 13 C EGCG δ 2 H EGCG and δ 18 O EGCG The preparation method of EGCG monomer involves making tea powder from tea samples, extracting and purifying epigallocatechin gallate (EGCG) monomer from the tea powder, and then determining the stable isotope ratio of the EGCG monomer. Based on the above stable isotope ratio, the origin of the tea sample is identified. This invention is mainly used for the accurate identification of tea origin, providing technical support for tea traceability and quality control.

The present invention proposes a method for determining the stable oxygen isotope ratio of water in honey, which belongs to the technical field of stable isotope analysis of food. The method comprises the following steps: 1) adding an organic reagent to a honey sample to be tested, mixing and standing, taking the upper layer of liquid to obtain a solution to be tested; 2) taking the solution to be tested obtained in step 1) and placing it in a bottle, after sealing, replacing the air in the bottle with high-purity carbon dioxide gas, and then placing the bottle containing the solution to be tested and high-purity carbon dioxide under constant temperature conditions to react to obtain a reaction solution; 3) using a gas chromatography-stable isotope ratio mass spectrometry system, after determining the stable oxygen isotope ratio of carbon dioxide in the reaction solution, calibrating with a standard product, and obtaining the stable oxygen isotope ratio of water in the honey sample to be tested. The present invention can be used for simply and quickly determining the stable oxygen isotope ratio of water in honey, and can be used for research and application of honey authenticity or adulteration detection.

The invention relates to the technical field of detection, and discloses a method for tracing the origin of silkworm cocoons based on a stable isotope technology in combination with a machine learning algorithm, and the method comprises the steps: (1) collecting data; (2) data preprocessing; (3) model optimization; (4) model training; and (5) producing area tracing. According to the invention, origin traceability of silkworm cocoons from different origins is realized based on isotope analysis and a machine learning algorithm. Specifically, hydrogen, oxygen, carbon and nitrogen stable isotope ratio data are combined with KNN, SVM and RF algorithms to realize rapid and high-precision source tracing of silkworm cocoon producing areas. According to the invention, by combining the advantages of various machine learning algorithms, the accuracy and reliability of silkworm cocoon origin tracing are improved.

The invention discloses a method for identifying the producing area of saffron crocus by extracting saffron crocus aldehyde and measuring the stable isotope ratio of the saffron crocus aldehyde. The invention discloses a method for extracting crocetin from saffron crocus and measuring the stable isotope ratio of carbon to hydrogen to oxygen. The method comprises the following steps: S1, determining the stable isotope ratio of carbon to hydrogen to oxygen of a crocetin standard substance by using an elemental analysis-stable isotope mass spectrometer; s2, extracting crocus sativus aldehyde from the crocus sativus sample to obtain a crocus sativus aldehyde extracting solution; s3, determining the carbon, hydrogen and oxygen stable isotope ratio of crocetin in the crocetin extracting solution by using a gas chromatography-stable isotope mass spectrometer; and S4, calibrating the carbon, hydrogen and oxygen stable isotope ratio of crocetin in the crocetin extracting solution by adopting the carbon, hydrogen and oxygen stable isotope ratio of the crocetin standard substance. The standard substance is used for calibrating the stable isotope ratio of carbon, hydrogen and oxygen of the single compound crocetin, and the method can be used for identifying the producing area of crocetin.

The present invention discloses a method for tracing the spread of invasive alien plants using stable isotopes, which includes tracing the source of invasive alien plants and identifying the risk of invasive alien plant spread. The tracing the source of invasive alien plants includes S1, plant sample collection, S2, sample pretreatment, S3, stable isotope determination, S4, analysis of the spread source and spread path of invasive alien plants; the risk identification of invasive alien plants includes P1, construction of a spread network of invasive alien plants, and P2, identification of spread risk areas. The present invention measures the stable isotope ratios of plants from newly introduced areas and potential source areas, and combines a multi-dimensional analysis method to trace the source and spread path of invasive alien plants, with high tracing accuracy. Compared with traditional genetic analysis methods, this method has lower costs and higher experimental efficiency, and can significantly reduce tracing costs.

The invention belongs to the technical field of rice traceablility, and provides a traceablility model construction method and traceablility method for city-level, provincial-level and inter-provincial-level rice. According to the method for constructing the traceability model of the city-level rice, the stable isotope ratio information, the near infrared spectrum information, the mineral element information and the metabonomics information are fused in the middle level for the model of a small area, and the accuracy of the model is improved. When the provincial rice traceability model is constructed, the stable isotope ratio information and the near infrared spectrum information are fused in the middle stage, and the standardization processing method and the modeling mode are combined, so that the accuracy of the provincial rice traceability model is improved. When the traceability model of the inter-provincial rice is constructed, the stable isotope ratio information and the near infrared spectrum information are fused in the middle stage, and the accuracy of the traceability model of the inter-provincial rice is improved by combining a standardized processing method and a modeling mode.

The invention discloses an enrichment and purification device and method for testing stable isotopes of trace dissolved nitrogen in a water body, and relates to the field of stable isotope analysis and testing. Comprising a device for oxidizing nitrogen elements dissolved in water, a gas purification device, a nitrogen oxide freezing enrichment device, a micro nitrogen oxide reduction device, a nitrogen separation and purification device and a stable isotope ratio mass spectrometer which are arranged in sequence. According to the method, trace dissolved nitrogen in the water body is oxidized into nitrogen oxide at high temperature, the generated nitrogen oxide is subjected to liquid nitrogen freezing enrichment, enriched sample gas is reduced into nitrogen gas through a miniature reduction tube, and then the nitrogen gas is introduced into a stable isotope ratio mass spectrometer, so that the trace dissolved nitrogen isotope in the water body is tested.

The invention discloses a method for monitoring carbon leakage in a carbon dioxide mineralization process by using stable isotopic tracing. The method comprises the following steps: measuring a delta 13C value of a CO2 gas source used in the carbon dioxide mineralization process; a CO2 gas source is injected into the carbon dioxide mineralization process, and then the carbon dioxide mineralization process flow is started; collecting each key point sample in the carbon dioxide mineralization process; measuring and analyzing the delta 13C value of the sample at each key point; and calculating the carbon leakage rate according to the measured delta 13C value of the sample at each key point, and evaluating the mineralization effect. According to the method, CO2 migration and leakage risks are tracked in real time by injecting CO2 with a specific carbon stable isotope ratio (delta13C) and combining on-site laser spectrum analysis and multi-isotope detection, the monitoring precision and the quantification capacity are improved, the method is suitable for a carbon dioxide mineralization utilization project, and the environmental risk is reduced.

The present invention discloses a method for analyzing the sources of nitrogen pollution in mangroves based on the stable nitrogen isotope composition, which includes: culturing mangrove plants in the laboratory to ensure that the initial isotope values of each plant are consistent; placing cages in a series of mangroves with nitrogen concentration gradients and planting the mangrove plants into the cages; allowing the mangrove plants to grow in the cages for at least one month to ensure isotope integration of the mangrove plants; collecting mangrove plant samples from the cages multiple times, drying the mangrove plant samples, and grinding them into powders to make aluminum-tin capsules; using a stable isotope ratio mass spectrometer (IRMS) to analyze the δ<supgt;15< / supgt;N isotope ratio of the aluminum-tin capsules; drawing spatial variation diagrams, temporal variation diagrams, and hotspot diagrams based on the δ<supgt;15< / supgt;N isotope ratio; and determining the sources of nitrogen pollution according to the differences in the δ<supgt;15< / supgt;N isotope ratio of the mangrove plants. Through the method of the present invention, accurate judgment of the sources of nitrogen pollution in mangroves can be achieved, and it can also provide directions for environmental governance and environmental protection.

The invention discloses a pretreatment method of a nitrate nitrogen stable isotope sample, and belongs to the technical field of environmental science, agriculture and chemical analysis. The pretreatment method of the nitrate nitrogen stable isotope sample comprises the following steps: adding an excessive stannous chloride hydrochloric acid aqueous solution into a nitrate-containing water sample to reduce nitrate in the water sample into nitrite, and continuously adding excessive metal zinc to reduce the nitrite into N2O to obtain a nitrate nitrogen stable isotope sample to be detected. The pretreatment method of the nitrate nitrogen stable isotope sample provided by the invention is efficient, simple, convenient and accurate. And determining the nitrogen isotope ratio of the treated N2O by using a stable isotope ratio mass spectrometer which only responds to the N2O, and deducing the composition of the nitrogen isotope in the nitrate sample.

The present application belongs to the technical field of environmental monitoring, and particularly relates to a quantitative identification method for contribution of autochthonous organic carbon of surface water. The present application constructs a water DOM source analysis method based on stable isotope ratio and three-dimensional fluorescence spectrum, comprehensively analyzes the source and characteristics of DOM, and identifies the characteristic information and contribution of autochthonous organic carbon. The three-dimensional fluorescence spectrum in the present application has the advantages of rapid detection, small sample consumption, etc., and can quickly qualitatively identify the source of DOM. The stable isotope ratio analysis in the present application can quantitatively calculate the different sources of DOM, and combined with the land use, hydrology and water environment and water quality parameters of the research area, the source and migration rule of water DOM can be more comprehensively determined. The quantitative identification method provided by the present application is more comprehensive for water DOM research, the identification system is more complete, and the tracing result is more reliable.

The invention provides a method for detecting fossil carbon components in sewage organic matters based on wet chemical oxidation and carbon-13 isotope detection. The method comprises the following steps: 1, collecting a sewage sample, and adding phosphoric acid to adjust the pH value for pretreatment so as to inhibit microbial activity and remove soluble inorganic carbonate; step 2, taking enough persulfate solution as an oxidizing agent and transition metal salt solution as a catalyst, heating the sample in a water bath in a closed device with good air tightness to completely oxidize organic matters, and collecting generated CO2 gas; and 3, introducing the collected gas sample into a stable isotope ratio mass spectrometer, and determining the carbon-13 isotope abundance in the gas sample so as to calculate the proportion of fossil carbon in the sewage organic matters. The method can effectively determine the fossil carbon content in the sewage organic matters, is low in detection difficulty and cost, and is suitable for carbon emission reduction self-detection in the sewage treatment field.

The application provides a method and device for determining photosynthetic rate of plant leaves based on carbon 13 isotope labeling, and belongs to the technical field of biology. 13 The application first places the leaves to be measured into an assimilation chamber and labels them with CO2, and then measures the change in C content of specific parts of the leaves before and after labeling by means of an elemental analyzer and a stable isotope ratio mass spectrometer. 13 The photosynthetic rate of the leaves in unit area and unit time can be calculated. 13 The amount of C, i.e. the photosynthetic rate. The method and device can simultaneously determine the photosynthetic rates of multiple parts of the leaves, and greatly improve the determination efficiency.

The invention discloses a reaction furnace capable of observing the change of a reaction column for element isotope analysis, which is mainly used for visualization and quantification of an oxidation-reduction process in an element-stable isotope ratio mass spectrometer and comprises a reaction furnace body, a reaction tube and an observation strip, the reaction tube is arranged in the reaction furnace body, an opening is formed in the surface of the reaction tube, a through hole is formed in the surface of the reaction furnace body, the observation strip is arranged between the through hole and the opening, the observation strip covers the through hole and the opening in a sealing manner, and the observation strip is made of a transparent material. By adopting the arrangement, the oxidation reduction process can be observed and quantified at any time during use, and the visualization and quantification of the ash content volume and the reduced copper residual degree are realized.

The invention provides a method for determining the oxygen isotope ratio of water in high-alcohol-content Baijiu, and relates to the field of stable isotope ratio mass spectrometry detection. The determination method comprises the following steps: diluting to-be-determined high-degree white spirit by using water with a known oxygen isotope ratio to obtain a diluted target spirit sample; performing equilibrium exchange reaction on 16O of water in the target wine sample and 18O of the carbon dioxide standard gas to obtain a reaction wine sample; measuring the oxygen isotope ratio of water in the reaction wine sample; and performing conversion to obtain the oxygen isotope ratio. According to the method, the high-degree white spirit is diluted with water with the known oxygen isotope ratio, the ethanol concentration is reduced to avoid interference of isotopic, and the original value is inversely calculated by combining the mass conservation principle. According to the method, tedious off-line physical separation is not needed, automatic analysis can be carried out by directly utilizing the on-line mass spectrometer, the operation process is greatly simplified, and the detection efficiency is remarkably improved while it is guaranteed that the result is accurate and reliable.

The application belongs to the field of analysis and detection, and particularly relates to a method for detecting the content of directly absorbed exogenous glycine of microorganisms in soil, which comprises the following steps: firstly, mixing a double-labeled glycine solution with soil, adding sodium laureth sulfate and chloroform for fumigation, then extracting, deriving by hydrocarbonation reaction of ethyl bromoformate, and completing the extraction of glycine in microorganisms after stable layering by using sodium bicarbonate solution; and after the extraction is cracked by a stable isotope ratio mass spectrometer, exogenous glycine and original glycine in microorganisms are distinguished by characteristic ions to be determined. The method can effectively detect the content of directly absorbed exogenous glycine of microorganisms, is simple in operation, strong in specificity, has a good linear relationship, high sensitivity and accuracy, and effectively solves the problem that the content of directly absorbed exogenous glycine of microorganisms cannot be directly quantified. Thus, the direct utilization pathway of soil microorganisms to exogenous amino acid substances is quantitatively judged.

The present invention discloses a method for detecting the stable carbon isotope value of ethyl hexanoate, and belongs to the technical field of carbon isotope analysis. In a system where the stable carbon isotope values ​​of ethanol and hexanoic acid are known, when the three substances of ethanol, hexanoic acid and ethyl hexanoate are in a dynamic equilibrium state, a stable isotope ratio mass spectrometer is used to determine the stable carbon isotope value of ethyl hexanoate, and a multivariate linear regression analysis is performed on the stable carbon isotope values ​​of ethanol, hexanoic acid and ethyl hexanoate to obtain a calculation formula for the stable carbon isotope value of ethyl hexanoate based on the stable carbon isotope values ​​of ethanol and hexanoic acid. The method of the present invention is simple to operate, and the test δ 13 C value and calculation of δ 13 There is no obvious difference in C value, which has the advantages of high efficiency and accuracy. 13 C. Hexanoic acid delta 13 C, ethyl hexanoate δ 13 The research on C provides new ideas.

The application belongs to the field of analysis and detection, and particularly relates to a method for detecting the content of directly absorbed exogenous glycine of microorganisms in soil, which comprises the following steps: firstly, mixing a double-labeled glycine solution with soil, adding sodium laureth sulfate and chloroform for fumigation, then extracting, deriving by hydrocarbonation reaction of ethyl bromoformate, and completing the extraction of glycine in microorganisms after stable layering by using sodium bicarbonate solution; and after the extraction is cracked by a stable isotope ratio mass spectrometer, exogenous glycine and original glycine in microorganisms are distinguished by characteristic ions to be determined. The method can effectively detect the content of directly absorbed exogenous glycine of microorganisms, is simple in operation, strong in specificity, has a good linear relationship, high sensitivity and accuracy, and effectively solves the problem that the content of directly absorbed exogenous glycine of microorganisms cannot be directly quantified. Thus, the direct utilization pathway of soil microorganisms to exogenous amino acid substances is quantitatively judged.