Device and method for tracing and analyzing nitrate in water body based on stable isotope analysis technology

Abstract

The invention discloses a stable isotope analysis technology-based traceability analysis device for nitrate in a water body, and the device comprises a workbench which is rotatably provided with a rotating seat and is fixedly provided with a support, and the rotating seat is provided with a plurality of groups of through holes for installing sample containers in an annular array; the mounting plate is arranged above the rotating seat and is driven by telescopic equipment to move up and down, a copper-plated cadmium particle storage device, an azide acid solution storage device and a stable isotope ratio mass spectrometer are arranged on the mounting plate, and the discharge end of the copper-plated cadmium particle storage device and the discharge end of the azide acid solution storage device are communicated with a first quantity control part and a second quantity control part respectively. The sample container can correspond to the quantity control part I, the quantity control part II and a butt joint pipe in the stable isotope ratio mass spectrometer through rotation of the rotating seat, so that copper-plated cadmium particles and an azide acid solution are added, detection is carried out through the stable isotope ratio mass spectrometer, the mode is suitable for the condition that the number of water samples is large, and the detection accuracy is high. And the efficiency of traceability analysis work is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of nitrate source tracing and analysis, and specifically to a device and method for tracing and analyzing nitrates in water bodies based on stable isotope analysis technology. Background Art

[0002] Nitrates can easily lead to the acidification of the water ecosystem, stimulate the growth and proliferation of primary producers, cause eutrophication of the water ecosystem, and even reach a toxic level that harms the survival of aquatic animals. To reduce nitrate input and improve the water ecosystem status of polluted water bodies, it is crucial to accurately identify the sources of nitrate pollution in water bodies. Currently, stable isotope analysis technology has become an effective technical means for identifying the sources of nitrate pollution in water bodies.

[0003] Stable isotope analysis technology uses a stable isotope mass spectrometer to measure the nitrogen isotopes of nitrates. Usually, the water body needs to be pretreated before measurement. Common pretreatment methods include chemical methods and bacterial denitrification methods. Among them, the chemical method is more widely used. The chemical method uses copper-coated cadmium granules to convert nitrates into nitrites, and then uses hydrazoic acid to convert nitrites into N2O. Subsequently, N2O is detected by a stable isotope mass spectrometer. However, this method usually requires manual addition of copper-coated cadmium granules and hydrazoic acid into a test tube containing a water sample, and then manual shaking. When the number of water samples is large, this method seriously affects the efficiency of the entire water body source tracing and analysis work. At the same time, since copper-coated cadmium granules and hydrazoic acid are toxic to a certain extent, this method is also likely to pose health hazards and safety risks to operators. Therefore, we propose a device and method for tracing and analyzing nitrates in water bodies based on stable isotope analysis technology. Summary of the Invention

[0004] The purpose of the present invention is to provide a device and method for tracing and analyzing nitrates in water bodies based on stable isotope analysis technology to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions:

[0006] A device for tracing and analyzing nitrates in water bodies based on stable isotope analysis technology, comprising:

[0007] A workbench, on which a rotating seat is rotatably provided, and a bracket is fixedly provided. A plurality of groups of through holes for installing sample containers are arranged in a circular array on the rotating seat;

[0008] The mounting plate is arranged above the rotating seat and is driven to move up and down by a telescopic device. The telescopic device is arranged on the bracket. The mounting plate is provided with a copper-plated cadmium granule storage, an azoic acid solution storage, and a stable isotope ratio mass spectrometer. The discharging ends of the copper-plated cadmium granule storage and the azoic acid solution storage are respectively communicated with a first metering component and a second metering component for controlling the discharging amount. The discharging ends of the first metering component and the second metering component and the air inlet end of the stable isotope ratio mass spectrometer are all communicated with a docking pipe. The first metering component and the second metering component are driven to work by a driving component;

[0009] Every time the rotating seat rotates a preset angle, each docking pipe corresponds to a sample container on the rotating seat, so as to insert into the corresponding sample container when the mounting plate moves downward.

[0010] The further improvement lies in that the first metering component includes:

[0011] A housing, the upper and lower ends of which are respectively provided with a feed inlet and a discharge outlet communicated with the copper-plated cadmium granule storage and the docking pipe. A metering disk adapted thereto is rotatably arranged in the inner cavity thereof. The shaft part of the metering disk is connected with the driving component. A plurality of groups of particle grooves for accommodating a copper-plated cadmium granule are arranged on the circumferential outer wall of the metering disk.

[0012] The further improvement lies in that the second metering component includes:

[0013] A transparent cylinder, inside which a piston adapted thereto is movably arranged. Scale lines are arranged on the outer wall of the transparent cylinder. One end of the transparent cylinder is communicated with the discharging end of the azoic acid solution storage through a first one-way pipeline, and the other end is communicated with the docking pipe through a second one-way pipeline;

[0014] An adjusting screw rod is rotatably arranged in the transparent cylinder and movably penetrates through the piston. One end of the adjusting screw rod extends to the outer end of the transparent cylinder and is connected with a hand wheel. A limiting block for restricting the moving position of the piston is threadedly sleeved on one side of the outer wall of the adjusting screw rod and inside the transparent cylinder;

[0015] A magnetic pull rod, one end of which is connected with the piston, and the other end movably penetrates through the limiting block and the other end of the transparent cylinder and then extends to the outside of the transparent cylinder, and is driven to move by the driving component. A reset elastic component for driving the piston to reset is sleeved on the outer wall of the magnetic pull rod.

[0016] The further improvement lies in that the driving component includes:

[0017] A protective shell is arranged on the mounting plate and movably sleeved on one end of the shaft part of the metering disk;

[0018] A second rotating device is arranged on the protective shell. The output end of the second rotating device extends into the protective shell and is in transmission connection with one end of the shaft part of the metering disk through a bevel gear set;

[0019] The electromagnetic ring is fixedly sleeved on the outer wall of the protective shell and is used for electrifying and adsorbing the magnetic pull rod.

[0020] A further improvement lies in that the sample container includes:

[0021] A container body with a hollow top, a sealing cover is detachably provided at the top thereof, a through port for inserting a docking pipe is opened on the sealing cover, and a discharge port is opened on the side wall of the end of the docking pipe inserted into the through port;

[0022] A closing block is attached to the bottom of the sealing cover and is used for closing the through port. The closing block is connected to the sealing cover through an elastic connecting piece.

[0023] A further improvement lies in that an arc-shaped groove is opened at the bottom of the container body, a stirring member is rotatably provided in the container body, one end of the stirring member extends into the arc-shaped groove, a torsion spring is provided at the connection between the stirring member and the container body, a pulling rope is wound around one end of the stirring member, one end of the pulling rope is connected to a magnetic arc-shaped slider, the magnetic arc-shaped slider is slidably provided in the arc-shaped groove, and the magnetic arc-shaped slider is connected to one inner wall of the arc-shaped groove through a connecting elastic member;

[0024] A driven disk is rotatably provided at the bottom of the rotating seat, the driven disk is rotatably provided on the workbench, a transmission telescopic rod is connected to the top of the driven disk, one end of the transmission telescopic rod movably penetrates through the rotating seat and is connected to the driving member, and is driven to rotate by the driving member. A plurality of groups of magnetic blocks for adsorbing the magnetic arc-shaped slider are embedded in the top of the driven disk in an annular array. When the magnetic block corresponds to the magnetic arc-shaped slider, it adsorbs the magnetic arc-shaped slider, and drives the magnetic arc-shaped slider to move in the arc-shaped groove when the driven disk rotates. When the magnetic arc-shaped slider moves, it drives the stirring member to rotate through the pulling rope. When the magnetic block and the magnetic arc-shaped slider are separated, the stirring member rotates back to its original position under the action of the torsion spring.

[0025] A further improvement lies in that a rotating device one for driving the rotating seat to rotate is provided on the workbench.

[0026] A further improvement lies in that a counter is provided on the outer wall of the docking pipe connected to the metering member one for measuring the number of copper-plated cadmium particles discharged from the docking pipe. The counter and the stable isotope ratio mass spectrometer are both electrically connected to a display screen. The counter, the telescopic device and the driving member are all electrically connected to a controller. When the number of discharged copper-plated cadmium particles reaches a threshold value, the counter sends a signal to the controller, so that the controller controls the telescopic device to drive the mounting plate to move upward and makes the driving member turn off.

[0027] A further improvement lies in that a solenoid valve is further provided in the docking pipe.

[0028] A method for tracing and analyzing nitrates in water based on stable isotope analysis technology, using the above-mentioned analysis device, includes the following steps:

[0029] S1: Place a water sample in a sample container, and then put it into the through hole on the rotating seat.

[0030] S2: The rotating seat rotates. When the sample container corresponds to the first metering component, drive the mounting plate to descend through the telescopic device, so that the docking pipe on the first metering component enters the sample container. Through the first metering component, the copper-coated cadmium particles in the copper-coated cadmium particle storage are quantitatively introduced into the sample container to be mixed with the water sample, converting the nitrate in the water sample into nitrite. Then drive the mounting plate to rise through the telescopic device, and then rotate the rotating seat until the sample container corresponds to the second metering component. Drive the mounting plate to descend through the telescopic device, so that the docking pipe on the second metering component enters the sample container. Through the second metering component, the hydrazoic acid solution in the hydrazoic acid solution storage enters the sample container to be mixed with the water sample, converting the nitrite into NO. Then repeat the above operation, and detect the NO in the sample container by a stable isotope ratio mass spectrometer to obtain the nitrogen-oxygen isotope ratio data.

[0031] S3: Compare the nitrogen-oxygen isotope ratio data with the isotope fingerprint database of known pollution sources, and calculate the contribution ratio of each pollution source through the calculation ratio module to determine the source of water body nitrate pollution.

[0032] Compared with the prior art, the beneficial effects of the present invention are:

[0033] The present invention can make the sample container containing the water sample correspond to the docking pipes in the first metering component, the second metering component and the stable isotope ratio mass spectrometer respectively by rotating the rotating seat. Thus, the copper-coated cadmium particles and the hydrazoic acid solution can be quantitatively added into the sample container through the first metering component and the second metering component respectively, and then the nitrate in the sample container is converted into N2O and detected by the stable isotope ratio mass spectrometer. This method is applicable to the situation where the number of water samples is large, improving the efficiency of the whole water body tracing and analysis work. At the same time, the copper-coated cadmium particles and the hydrazoic acid solution are automatically quantitatively added, avoiding health hazards and safety hazards to the operators and reducing the waste of the copper-coated cadmium particles and the hydrazoic acid solution. And when the first metering component and the second metering component work, the driven disk is also rotated. When the driven disk rotates, the stirring component works through structures such as magnetic blocks, pull ropes, magnetic arc sliders and connecting elastic members, stirring the liquid in the sample container evenly, further improving the efficiency of the whole tracing and analysis work. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic structural diagram of the analysis device of the present invention;

[0035] Figure 2 For the present invention Figure 1 Another perspective structural diagram;

[0036] Figure 3Partial cross-sectional view of the metering part 1 and metering part 2 of the present invention;

[0037] Figure 4 Cross-sectional view of the sample container structure of the present invention;

[0038] Figure 5 Schematic diagram of the structure of the driven disk of the present invention;

[0039] Figure 6 Schematic diagram of the structure of the sample container from another perspective of the present invention.

[0040] In the figure: 1, workbench; 2, rotating seat; 3, rotating device 1; 4, sample container; 41, container body; 42, sealing cover; 43, arc groove; 44, stirring member; 45, pull rope; 46, magnetic arc slider; 47, connecting elastic member; 48, closing block; 49, elastic connecting member; 5, bracket; 6, mounting plate; 7, copper-cadmium granule storage; 8, hydrazoic acid solution storage; 9, stable isotope ratio mass spectrometer; 10, telescopic device; 11, metering part 1; 111, housing; 112, metering disk; 113, particle groove; 12, metering part 2; 121, transparent cylinder; 122, piston; 123, adjusting screw; 124, limit block; 125, magnetic pull rod; 126, reset elastic member; 13, rotating device 2; 14, protective shell; 15, electromagnetic ring; 16, docking pipe; 17, discharge port; 18, driven disk; 19, transmission telescopic rod; 20, magnetic block; 21, display screen; 22, counter. Specific embodiments

[0041] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0042] Embodiment 1

[0043] Please refer to the attached Figure 1 - attached Figure 2 , a device for tracing and analyzing nitrate in water based on stable isotope analysis technology, comprising:

[0044] A workbench 1 is provided with a rotating seat 2 for rotation and a bracket 5 for fixed installation. The rotating seat 2 is provided with a plurality of through holes for mounting a sample container 4 in a circular array. Furthermore, the workbench 1 is provided with a rotating device 3 for driving the rotating seat 2 to rotate. The rotating device 3 includes a motor and a gear set (two sets of meshing gears) for transmission connection between the motor output end and the rotating seat 2. The rotating seat 2 can be controlled to intermittently rotate at a preset angle by the rotating device 3 to attach Figure 1 As shown, there are four groups of through holes, and the rotating device 3 controls the rotating seat 2 to rotate 90 degrees each time;

[0045] The mounting plate 6 is arranged above the rotating seat 2 and is driven to move up and down by a telescopic device 10 (such as an electric telescopic rod). The telescopic device 10 is arranged on the bracket 5. The mounting plate 6 is provided with a copper-plated cadmium particle storage 7, a hydrazoic acid solution storage 8 and a stable isotope ratio mass spectrometer 9. The copper-plated cadmium particle storage 7 is used to store copper-plated cadmium particles, the hydrazoic acid solution storage 8 is used to store hydrazoic acid solution, and the stable isotope ratio mass spectrometer 9 belongs to conventional instruments in the field and is not described in detail herein; the discharge ends of the copper-plated cadmium particle storage 7 and the hydrazoic acid solution storage 8 are respectively connected with a control unit 1 11 and a control unit 2 12 for controlling the discharge amount, and the discharge ends of the control unit 1 11 and the control unit 2 12 and the air inlet end of the stable isotope ratio mass spectrometer 9 are all connected with a docking pipe 16 for docking with the sample container 4;

[0046] Furthermore, a solenoid valve is also provided in the butt joint pipe 16. When there is only one set of sample containers 4, the copper-plated cadmium particles can be discharged into the sample container 4 by independently controlling the solenoid valve in the corresponding butt joint pipe 16. When there are two or more sets of sample containers 4, the solenoid valves in all butt joint pipes 16 can be opened.

[0047] The control element 11 and the control element 2 12 are driven to work by the driving element;

[0048] Each time the rotating base 2 rotates by a preset angle, each butt joint 16 corresponds to a sample container 4 on the rotating base 2, so as to be inserted into the corresponding sample container 4 when the mounting plate 6 moves downward;

[0049] This method facilitates efficient nitrate source tracing and analysis in water bodies without the need for users to manually add copper-plated cadmium particles, add hydrazoic acid solution, and perform detection by stable isotope ratio mass spectrometer 9. It is easy to use and avoids health hazards and safety risks to operators caused by copper-plated cadmium particles and hydrazoic acid solution.

[0050] A method for tracing the source of nitrate in water based on stable isotope analysis technology, using the above-mentioned analysis device, includes the following steps:

[0051] S1: Place the water sample in the sample container 4, and then put it into the through hole on the rotating seat 2;

[0052] S2: The rotating seat 2 rotates. When the sample container 4 corresponds to the first metering component 11, the telescopic device 10 is used to drive the mounting plate 6 to descend, so that the docking pipe 16 on the first metering component 11 enters the sample container 4. Through the first metering component 11, the copper-coated cadmium grains in the copper-coated cadmium grain storage 7 are quantitatively introduced into the sample container 4 to be mixed with the water sample, converting the nitrate in the water sample into nitrite. Then, the telescopic device 10 is used to drive the mounting plate 6 to rise, and then the rotating seat 2 rotates until the sample container 4 corresponds to the second metering component 12. The telescopic device 10 is used to drive the mounting plate 6 to descend, so that the docking pipe 16 on the second metering component 12 enters the sample container 4. Through the second metering component 12, the hydrazoic acid solution in the hydrazoic acid solution storage 8 enters the sample container 4 to be mixed with the water sample, converting the nitrite into N2O. Then, repeat the above operation, and detect the N2O in the sample container 4 by the stable isotope ratio mass spectrometer 9 to obtain the nitrogen-oxygen isotope ratio data;

[0053] S3: Compare the nitrogen-oxygen isotope ratio data with the isotope fingerprint database of known pollution sources, and calculate the contribution ratio of each pollution source through the calculation ratio module to determine the source of water body nitrate pollution;

[0054] The above-mentioned isotope fingerprint database of known pollution sources refers to a database that records and stores the proportions or characteristics of stable isotopes in pollution sources, which belongs to the prior art and will not be elaborated here;

[0055] The calculation ratio module is a conventional module in the art. The calculation ratio module is a tool based on the isotope mixing model. By comparing the measured nitrogen-oxygen isotope ratio with the isotope fingerprint database of known pollution sources, combined with the mass balance equation and Bayesian probability method, it quantifies the contribution ratio of each pollution source (such as agricultural fertilizers, domestic sewage, industrial emissions, atmospheric deposition) to the water body nitrate pollution. This module usually adopts stable isotope analysis models (such as SIAR, MixSIAR) or IsoSource models. By inputting the measured isotope values, pollution source isotope characteristics (mean ± standard deviation), and isotope fractionation coefficients (optional), it uses Markov chain Monte Carlo (MCMC) simulation to generate the probability distribution of the contribution ratio of each pollution source, and outputs the contribution ratio and its confidence interval (such as agricultural fertilizers accounting for 40%, and the 95% confidence interval is 35 - 45%). Through this module, it can be upgraded from qualitatively identifying pollution sources to quantitatively analyzing the contribution ratio of each pollution source, providing a scientific basis and priority decision-making support for the treatment of water body nitrate pollution. Of course, the above-mentioned calculation ratio module is not limited to this one.

[0056] Example 2

[0057] Please refer to the attached Figure 3 , on the basis of Embodiment 1, the metering member 11 of this embodiment includes:

[0058] A housing 111, with a feed port and a discharge port 17 respectively provided at its upper and lower ends and communicating with the copper-plated cadmium particle storage 7 and the docking pipe 16. The vertical cross-section of the inner cavity of the housing 111 is circular, and a matching metering disk 112 is rotatably provided in its inner cavity. The shaft portion of the metering disk 112 is connected to a driving member, and a plurality of groups of particle grooves 113 for accommodating a copper-plated cadmium particle are provided on the circumferential outer wall of the metering disk 112;

[0059] When the docking pipe 16 of the metering member 11 corresponds to the sample container 4, the driving member is used to drive the metering disk 112 to rotate. The metering disk 112 picks up a copper-plated cadmium particle discharged from the discharge end of the copper-plated cadmium particle storage 7 through the particle groove 113 and the feed port. When the particle groove 113 corresponds to the docking pipe 16, the copper-plated cadmium particle located in the particle groove 113 enters the docking pipe 16 from the discharge port 17, and then enters the sample container 4 from the docking pipe 16 to be mixed with the water sample, converting nitrate in the water sample into nitrite.

[0060] Preferably, the metering member 12 of this embodiment includes:

[0061] A transparent cylinder 121, with a matching piston 122 movably provided inside it. Scale lines are provided on the outer wall of the transparent cylinder 121. One end of the transparent cylinder 121 is connected to the discharge end of the azoic acid solution storage 8 through a one-way pipeline one, and the other end is connected to the docking pipe 16 through a one-way pipeline two. Both the one-way pipeline one and the one-way pipeline two are pipelines with one-way valves;

[0062] An adjusting screw 123, rotatably provided inside the transparent cylinder 121 and movably passing through the piston 122. One end of the adjusting screw 123 extends to the outer end of the transparent cylinder 121 and is connected to a handwheel. A limiting block 124 for restricting the moving position of the piston 122 is threadedly sleeved on the outer wall of the adjusting screw 123 and on the side inside the transparent cylinder 121. Initially, observe the scale line and rotate the handwheel to drive the limiting block 124 to move inside the transparent cylinder 121 and adjust it to the required position. It should be noted that vent holes are provided on both the limiting block 124 and the outer wall of the end of the transparent cylinder 121 away from the adjusting screw 123, so that when the piston 122 moves, the outside air can enter and exit the transparent cylinder 121 to maintain the air pressure balance inside the transparent cylinder 121;

[0063] A magnetic pull rod 125, with one end connected to the piston 122 and the other end movably passing through the limiting block 124 and the other end of the transparent cylinder 121 and extending to the outside of the transparent cylinder 121, is driven to move by a driving member. A reset elastic member 126 for driving the piston 122 to reset is sleeved on the outer wall of the magnetic pull rod 125;

[0064] When the docking tube 16 of the control unit 12 corresponds to the sample container 4, the driving unit drives the magnetic pull rod 125 to move, and the magnetic pull rod 125 pulls the piston 122 to contact the limit block 124. When the piston 122 moves toward the limit block 124, the hydrazoic acid solution in the hydrazoic acid solution storage 8 is drawn into the transparent tube 121 through the one-way pipe 1. After the driving unit is closed, the magnetic pull rod 125 drives the piston 122 to reset under the action of the reset elastic member 126. The reset of the piston 122 compresses the hydrazoic acid solution in the transparent tube 121 to the docking tube 16 through the one-way pipe 2. Subsequently, the hydrazoic acid solution enters the sample container 4 through the docking tube 16 and mixes with the water sample to convert nitrite into N2O. The amount of hydrazoic acid solution entering the sample container 4 can be limited by adjusting the position of the limit block 124.

[0065] Preferably, the driving member of this embodiment includes:

[0066] The protective shell 14 is arranged on the mounting plate 6 and is movably sleeved on one end of the shaft of the control disk 112. A bearing is arranged at the connection between the protective shell 14 and the shaft of the control disk 112.

[0067] The second rotating device 13 is arranged on the protective shell 14. The second rotating device 13 includes a motor and a reducer. The output end of the second rotating device 13 extends into the protective shell 14 and is connected to one end of the shaft of the control disk 112 through a bevel gear set (two sets of combined bevel gears).

[0068] The electromagnetic ring 15 is fixedly sleeved on the outer wall of the protective shell 14 and is used for energizing and adsorbing the magnetic pull rod 125 (for example, made of metal material).

[0069] When the driving part is working, the rotating device 2 13 and the electromagnetic ring 15 are energized, and then the rotating device 2 13 drives the control disk 112 to rotate, and the electromagnetic ring 15 is energized to attract the magnetic pull rod 125, so that the magnetic pull rod 125 moves; when the driving part is closed, the rotating device 2 13 and the electromagnetic ring 15 are de-energized.

[0070] Example 3

[0071] Please see attached Figure 4 -Attached Figure 6 Based on Example 1, as a preference, the sample container 4 of this embodiment includes:

[0072] The top of the container body 41 is hollow, and a sealing cover 42 is detachably provided on the top. The sealing cover 42 and the container body 41 can be connected by threading or snapping. The sealing cover 42 is provided with a through hole for inserting the butt joint 16. The side wall of one end of the butt joint 16 inserted into the through hole is provided with a discharge port 17, and the bottom end of the butt joint 16 is closed.

[0073] The closing block 48 is attached to the bottom of the sealing cover 42 and is used to close the through port. The closing block 48 is connected to the sealing cover 42 through an elastic connecting member 49 (including a guide rod with one end connected to the bottom of the sealing cover 42 and movably passing through the closing block 48 and a spring sleeved on the outer wall of the guide rod. One end of the spring is connected to the bottom end of the guide rod and the other end is connected to the closing block 48).

[0074] When the docking pipe 16 is inserted downward into the through port, it squeezes the closing block 48 to move downward. As a result, a gap is generated between the closing block 48 and the sealing cover 42. At this time, the docking pipe 16 is communicated with the inner cavity of the container body 41 through the discharge port 17. When the docking pipe 16 moves upward, the closing block 48 is reset by the elastic connecting member 49 to close the through port, so as to prevent the liquid or gas in the container body 41 from escaping from the container body 41.

[0075] Preferably, an arc-shaped groove 43 is formed at the bottom of the container body 41 of this embodiment. A stirring member 44 (including a stirring rod and blades provided on the outer wall of the stirring rod) is rotatably provided in the container body 41. One end of the stirring member 44 extends into the arc-shaped groove 43. A torsion spring is provided at the connection between the stirring member 44 and the container body 41. A pull rope 45 is wound around one end of the stirring member 44. One end of the pull rope 45 is connected to a magnetic arc-shaped slider 46. The magnetic arc-shaped slider 46 (made of metal material, for example) is slidably provided in the arc-shaped groove 43. The magnetic arc-shaped slider 46 is connected to one side inner wall of the arc-shaped groove 43 through a connecting elastic member 47 (a spring, for example);

[0076] A driven disk 18 is rotatably provided at the bottom of the rotating seat 2 through a bearing. The driven disk 18 is rotatably provided on the workbench 1 through a bearing. A transmission telescopic rod 19 is connected to the top of the driven disk 18. The transmission telescopic rod 19 enables the mounting rod to move up and down while transmitting the rotational force of the driving member. The transmission telescopic rod 19 includes a sleeve rod and a movable rod inserted into one end of the sleeve rod. The movable rod and the sleeve rod can be slidably connected by means of a slider and a chute. One end of the transmission telescopic rod 19 movably passes through the rotating seat 2 and is connected to the driving member and is driven to rotate by the driving member. Specifically, the movable rod of the transmission telescopic rod 19 is connected to the output end of the rotating device II 13 of the driving member. A plurality of magnetic blocks 20 for adsorbing the magnetic arc-shaped slider 46 are embedded in the top of the driven disk 18 in an annular array. When the magnetic blocks 20 correspond to the magnetic arc-shaped slider 46, they adsorb the magnetic arc-shaped slider 46 and drive the magnetic arc-shaped slider 46 to move in the arc-shaped groove 43 when the driven disk 18 rotates. When the magnetic arc-shaped slider 46 moves, it pulls the stirring member 44 to rotate through the pull rope 45. When the magnetic blocks 20 and the magnetic arc-shaped slider 46 are separated, the stirring member 44 is reset to rotate under the action of the torsion spring;

[0077] When the driving part works, it also drives the driven disk 18 to rotate through the transmission telescopic rod 19. When the driven disk 18 rotates, the magnetic block 20 cooperates with the magnetic arc-shaped slider 46, so that the magnetic arc-shaped slider 46 moves and pulls the stirring part 44 to rotate through the pull rope 45. The rotation of the stirring part 44 makes the water body inside mix with the copper-plated cadmium particles or the hydrazoic acid solution, improving the reaction efficiency and uniformity.

[0078] Embodiment 4

[0079] Please refer to the attached Figure 4 - attached Figure 6 On the basis of Embodiment 1, a counter 22 is provided on the outer wall of the docking pipe 16 connected to the metering part 11 in this embodiment, which is used to measure the number of copper-plated cadmium particles discharged from the docking pipe 16. The counter 22 can be, for example, a pipeline particulate matter detector, etc., and of course it is not limited to this one. Both the counter 22 and the stable isotope ratio mass spectrometer 9 are electrically connected to the display screen 21. The display screen 21 can be arranged on the workbench 1. The counting value of the counter 22 and the nitrogen-oxygen isotope ratio data detected by the stable isotope ratio mass spectrometer 9 are displayed through the display screen 21. Both the counter 22 and the telescopic device 10 are electrically connected to the controller. When the number of discharged copper-plated cadmium particles reaches the threshold value, the counter 22 sends a signal to the controller, so that the controller controls the telescopic device 10 to drive the mounting plate 6 to move upward and turn off the driving part;

[0080] For example, set the counting threshold to 5. When the number of discharged copper-plated cadmium particles reaches 5, the counter 22 enables the controller to control the telescopic device 10 and the driving part. Then, the mounting plate 6 resets upward, so that the docking pipe 16 is separated from the sample container 4, and at the same time, no more copper-plated cadmium particles are discharged or N2O flows through the docking pipe 16.

[0081] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for tracing and analyzing nitrate in water based on stable isotope analysis technology, characterized in that, Including: A workbench (1) is rotatably provided with a rotating seat (2) thereon, and a bracket (5) is fixedly provided thereon. A plurality of groups of through holes for installing sample containers (4) are formed on the rotating seat (2) in an annular array. A mounting plate (6) is arranged above the rotating seat (2) and is driven to move up and down by a telescopic device (10). The telescopic device (10) is arranged on the bracket (5). A copper-coated cadmium granule storage (7), an azoic acid solution storage (8) and a stable isotope ratio mass spectrometer (9) are arranged on the mounting plate (6). The discharge ends of the copper-coated cadmium granule storage (7) and the azoic acid solution storage (8) are respectively communicated with a first metering member (11) and a second metering member (12) for controlling the discharge amount. The discharge ends of the first metering member (11), the second metering member (12) and the intake end of the stable isotope ratio mass spectrometer (9) are all communicated with a docking pipe (16). The first metering member (11) and the second metering member (12) are driven to work by a driving member. When the rotating seat (2) rotates a preset angle each time, each docking pipe (16) corresponds to a sample container (4) on the rotating seat (2) respectively, so as to insert into the corresponding sample container (4) when the mounting plate (6) moves downward.

2. The parsing device according to claim 1, characterized in that: The first metering member (11) includes: A housing (111) is respectively provided with a feed port and a discharge port (17) communicated with the copper-coated cadmium granule storage (7) and the docking pipe (16) at its upper and lower ends. A metering disk (112) adapted thereto is rotatably arranged in its inner cavity. The shaft part of the metering disk (112) is connected with the driving member. A plurality of groups of particle grooves (113) for accommodating a copper-coated cadmium granule are formed on the circumferential outer wall of the metering disk (112).

3. The parsing device according to claim 2, wherein: The second metering member (12) includes: A transparent cylinder (121) is internally provided with a piston (122) adapted thereto. Scale lines are arranged on the outer wall of the transparent cylinder (121). One end of the transparent cylinder (121) is communicated with the discharge end of the azoic acid solution storage (8) through a one-way pipeline one, and the other end is communicated with the docking pipe (16) through a one-way pipeline two. An adjusting screw rod (123) is rotatably arranged in the transparent cylinder (121) and movably penetrates through the piston (122). One end of the adjusting screw rod (123) extends to the outer end of the transparent cylinder (121) and is connected with a hand wheel. A limiting block (124) for limiting the moving position of the piston (122) is threadedly sleeved on the outer wall of the adjusting screw rod (123) and on one side inside the transparent cylinder (121). A magnetic pull rod (125) has one end connected with the piston (122), and the other end movably penetrates through the limiting block (124) and the other end of the transparent cylinder (121) and then extends to the outside of the transparent cylinder (121), and is driven to move by the driving member. A reset elastic member (126) for driving the piston (122) to reset is sleeved on the outer wall of the magnetic pull rod (125).

4. The parsing device according to claim 3, wherein: The driving member includes: A protective shell (14) is arranged on the mounting plate (6) and movably sleeved on one end of the shaft part of the metering disk (112). The second rotating device (13) is provided on the protective housing (14), and the output end of the second rotating device (13) extends into the protective housing (14) and is drivingly connected to one end of the shaft portion of the metering disc (112) through a bevel gear set; The electromagnetic ring (15) is fixedly sleeved on the outer wall of the protective housing (14) and is used for electromagnetically adsorbing the magnetic pull rod (125).

5. The parsing device according to claim 1, wherein: The sample container (4) includes: A container body (41) with a hollow top, and a sealing cover (42) is detachably provided at the top thereof. A through hole for inserting the docking pipe (16) is provided on the sealing cover (42), and a discharge port (17) is provided on the side wall of one end of the docking pipe (16) inserted into the through hole; A closing block (48) is attached to the bottom of the sealing cover (42) for closing the through hole, and the closing block (48) is connected to the sealing cover (42) through an elastic connecting member (49).

6. The parsing device according to claim 5, wherein: An arc-shaped groove (43) is provided at the bottom of the container body (41). A stirring member (44) is rotatably provided in the container body (41). One end of the stirring member (44) extends into the arc-shaped groove (43). A torsion spring is provided at the connection between the stirring member (44) and the container body (41). A pull rope (45) is wound around one end of the stirring member (44). One end of the pull rope (45) is connected to a magnetic arc-shaped slider (46). The magnetic arc-shaped slider (46) is slidably provided in the arc-shaped groove (43), and the magnetic arc-shaped slider (46) is connected to one inner wall of the arc-shaped groove (43) through a connecting elastic member (47); A driven disc (18) is rotatably provided at the bottom of the rotating seat (2). The driven disc (18) is rotatably provided on the workbench (1). A transmission telescopic rod (19) is connected to the top of the driven disc (18). One end of the transmission telescopic rod (19) movably penetrates through the rotating seat (2) and is connected to a driving member, and is driven to rotate by the driving member. A plurality of groups of magnetic blocks (20) for adsorbing the magnetic arc-shaped slider (46) are embedded in the top of the driven disc (18) in an annular array. When the magnetic block (20) corresponds to the magnetic arc-shaped slider (46), it adsorbs the magnetic arc-shaped slider (46), and drives the magnetic arc-shaped slider (46) to move in the arc-shaped groove (43) when the driven disc (18) rotates. When the magnetic arc-shaped slider (46) moves, it pulls the stirring member (44) to rotate through the pull rope (45). When the magnetic block (20) and the magnetic arc-shaped slider (46) are separated, the stirring member (44) rotates back to its original position under the action of the torsion spring.

7. The parsing device according to claim 1, wherein: A first rotating device (3) for driving the rotating seat (2) to rotate is provided on the workbench (1).

8. The parsing device according to claim 1, wherein: The outer wall of the docking pipe (16) connected to the metering member one (11) is provided with a counter (22) for measuring the number of copper-coated cadmium particles discharged from the docking pipe (16). Both the counter (22) and the stable isotope ratio mass spectrometer (9) are electrically connected to a display screen (21). The counter (22), the telescopic device (10) and the driving member are all electrically connected to a controller. When the number of discharged copper-coated cadmium particles reaches a threshold value, the counter (22) sends a signal to the controller, causing the controller to control the telescopic device (10) to drive the mounting plate (6) to move upward and turn off the driving member.

9. The parsing device according to claim 1, wherein: An electromagnetic valve is further provided in the docking pipe (16).

10. A method for tracing and analyzing nitrate in water based on stable isotope analysis technology, using the analysis device according to any one of claims 1-9, characterized in that: It includes the following steps: S1: Place a water sample in the sample container (4), and then put it into the through hole on the rotating seat (2); S2: The rotating seat (2) rotates. When the sample container (4) corresponds to the metering member one (11), the telescopic device (10) is used to drive the mounting plate (6) to descend, so that the docking pipe (16) on the metering member one (11) enters the sample container (4). Through the metering member one (11), the copper-coated cadmium particles in the copper-coated cadmium particle storage (7) are quantitatively introduced into the sample container (4) to be mixed with the water sample, converting the nitrate in the water sample into nitrite. Then, the telescopic device (10) is used to drive the mounting plate (6) to rise, and then the rotating seat (2) rotates until the sample container (4) corresponds to the metering member two (12). The telescopic device (10) is used to drive the mounting plate (6) to descend, so that the docking pipe (16) on the metering member two (12) enters the sample container (4). Through the metering member two (12), the azoic acid solution in the azoic acid solution storage (8) enters the sample container (4) to be mixed with the water sample, converting the nitrite into N2O. Then, repeat the above operation, and the N2O in the sample container (4) is detected by the stable isotope ratio mass spectrometer (9) to obtain the nitrogen-oxygen isotope ratio data; S3: Compare the nitrogen-oxygen isotope ratio data with the isotope fingerprint database of known pollution sources, and calculate the contribution ratio of each pollution source through the calculation ratio module to determine the source of water nitrate pollution.

Images