459 results about "Tea tree" patented technology

The invention provides an unmanned aerial vehicle tea tree disease detection method based on morphological perception, and belongs to the technical field of agricultural information and computer vision. The method comprises the steps of firstly collecting tea garden images and constructing a data set; then, a target detection network embedded with a differentiable morphological sensor module is constructed, and the differentiable morphological sensor module extracts multi-scale shape features by using differentiable morphological operation; an adversarial learning mechanism is introduced in training, and the distinguishing ability of the model on disease and health areas is enhanced through a discriminator; after the training is completed, mining a difficult case sample based on the cosine distance between the morphological characteristics and the disease prototype vector, and carrying out supplementary training; and finally, integrating a plurality of models with optimal performance, and generating a final detection result through weighted reasoning. The method effectively strengthens the perception capability of the model for the subtle morphological characteristics of the diseases, solves the problems of low disease detection precision and insufficient difficult sample learning in a complex tea garden background, and remarkably improves the detection accuracy and robustness.

The invention belongs to the technical field of carbon sink evaluation, and discloses a typical weather-oriented tea garden carbon sink dynamic profit and loss evaluation method and system. Historical meteorological information and historical carbon sink monitoring information of the tea garden are collected, and a typical weather type library of the tea garden is constructed; determining a typical weather type corresponding to the current day of the tea garden, and calculating a carbon sink fixed amount and a carbon sink output amount of the tea garden; generating a correction factor for correction according to the management activity information of the tea garden to obtain a net carbon sink value; generating a total score based on comprehensive analysis of the net carbon sink value, and evaluating the carbon sink profit and loss condition of the tea garden; the carbon sink meteorological influence parameters before and after the monitoring time point are collected for comprehensive analysis to calculate the carbon sink fixed quantity and the carbon sink output quantity, calculation deviation caused by parameter fluctuation at a single time point can be avoided, and therefore the method better fits the continuous physiological process of photosynthesis / respiration of the tea trees, and the detection accuracy is improved. And the calculation result of the carbon sink fixed quantity / output quantity is closer to the actual condition.

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention provides an external application medicine for incontinent dermatitis and a preparation method thereof. The composition is prepared from active ingredients such as a radix sophorae flavescentis extract, calamine, vitamin E, ceramide, a herba portulacae extract, glycyrrhizic acid, chitosan and colloidal oat powder according to a specific proportion. Moisturizing matrixes such as sodium hyaluronate and glycerol and functional components such as tea tree essential oil are supplemented. And through a preparation process of matching high-speed shearing with ultrasonic dispersion, uniform dispersion and stability of each component are ensured. Experimental results show that the composition shows a remarkable synergistic effect in the aspects of anti-inflammation, antibiosis, skin barrier repair and the like, the comprehensive relieving and repairing effect of the composition is superior to that of a conventional drug control, and all the components and a special preparation process are necessary for achieving the optimal effect. The product has no irritation to human skin, is good in safety, can be prepared into various dosage forms such as cream, hydrogel or spray, and is suitable for nursing and repairing sensitive skin.

The invention discloses a preparation method of CsPHT1; the invention discloses modification of selenium element absorbed by tea and application of the gene 3 to feed, and relates to the field of agricultural gene engineering. CsPHT1 (CsPHT1); the gene 3 is GenBank: OP219455.1, gene mutation of D144K and T150V is carried out on the gene 3, and the amino acid sequence after mutation is shown as SEQ ID No: 01; tea tree CsPHT1; 3, the cultivation of D144K and T150V gene mutation seedlings is improved by the gene. The new variety cultivated by the invention can improve the selenium content and the oxidation resistance of the selenized tea polysaccharide without being influenced by the change of pH 5-7; the selenium-supplementing feed is obtained by mixing the high-selenium tea leaves with the feed, and the requirements of the current high-end feed market are met.

The invention relates to the technical field of medical dressings, in particular to a bacteriostatic and anti-inflammatory wound waterproof spray based on natural plant extracts and a preparation method. The wound waterproof spray is prepared from the following raw materials in parts by weight: 0.1-5 parts of tea tree essential oil, 0.5-10 parts of a honeysuckle flower extract, 5-20 parts of medical-grade polyurethane, 10-30 parts of ethanol, 0.1-3 parts of a rosemary extract and the balance of medical deionized water, wherein the total amount is 100 parts. A sodium alginate-chitosan composite wall material is adopted to carry out microencapsulation encapsulation on a core active substance, so that the stability of the tea tree essential oil and the honeysuckle flower extract can be remarkably improved, the high activity can be kept in the storage period, drugs can be slowly released after the tea tree essential oil and the honeysuckle flower extract are in contact with wounds, long-acting bacteriostasis and inflammation diminishing are achieved, and the wound healing effect is improved. The protection period and the treatment effect of single-time spraying are prolonged, meanwhile, the extremely mild characteristic is ensured, and potential stimulation and damage to wound tissue are avoided.

The invention provides plant-based sterilizing and acarus-killing shower gel for pets and a preparation method of the plant-based sterilizing and acarus-killing shower gel, and relates to the technical field of pet supplies. The plant-based sterilizing and acarus-killing shower gel for pets is prepared from the following components in percentage by mass: 3 to 8 weight percent of chinaberry seed extract, 1 to 3 weight percent of tea tree essential oil, 2 to 5 weight percent of thyme extract, 2 to 6 weight percent of wormwood extract, 8 to 15 weight percent of saponin plant extract, 2 to 4 weight percent of beta-cyclodextrin inclusion compound, 0.5 to 1.5 weight percent of sodium hyaluronate, 15 to 20 weight percent of amino acid surfactant, 5 to 10 weight percent of alkyl glycoside and the balance of water. The facial cleanser is prepared from, by weight, 3%-8% of cocamidopropyl betaine, 0.2%-0.8% of citric acid, 0.5%-2% of plant polysaccharide, 0.1%-0.5% of vitamin E, 2%-5% of glycerinum and the balance deionized water. Through synergistic compatibility of ternary plant active ingredients, application of a beta-cyclodextrin microencapsulation technology, selection of an amino acid surfactant and accurate control of the pH value, on the premise that no chemical preservative is added, perfect combination of efficient sterilization and mite removal, mildness, low irritation, lasting protection and excellent stability is achieved, and the effect of killing acarus is achieved. The method has important practical value and wide market prospect.

The invention discloses a bacillus velezensis YP-5 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a preservation number, a fungicide, a method for improving the planting capacity of bacillus velezensis in tea tree leaves and specific application. The bacillus velezensis YP-5 is high in control effect and wide in control spectrum. In addition, under the technological conditions of the invention, the quantity of colonized bacteria on tea tree leaves is increased by 1.9 times compared with that of a blank control group; after 7 days of treatment, the fixed planting bacteria quantity is 3.42 times of that of a blank control group. Meanwhile, when the microbial agent is sprayed to the tea trees in an indoor environment, the biological control effect on the tea anthracnose can be remarkably enhanced, and the biological control effect is improved by 10.29% compared with that of original YP-5 bacterial liquid. The problem that in the prior art, microbial thalli are weak in adhesion and prone to loss, and consequently the biocontrol capacity of the microbial thalli is weakened is effectively solved, and the method is suitable for industrial application of agricultural microbial preparations.

The invention provides a formula fertilizer special for tea trees, and relates to the technical field of tea tree fertilizers. According to the precise fertilization method of the special formula fertilizer for the tea trees, the dosage proportion of the fertilizer used for root fertilization and leaf surface fertilization can be determined according to the growth condition of the tea trees, the method adopts a'root + crown 'combined mode, fertilization is divided into root fertilization and leaf surface fertilization, and the fertilization efficiency is greatly improved. The full-water-soluble formula fertilizer special for the tea trees is capable of providing land size on-site accurate formula adjustment in real time; therefore, the yield and the quality of the tea leaves are improved, and the production benefits of the tea leaves are improved.

The invention relates to the technical field of agricultural fertilizers. More specifically, the invention relates to an extraction method of tenebrio molitor antibacterial peptide and application of the tenebrio molitor antibacterial peptide in preparation of tea tree anthracnose resistant fertilizer. According to the preparation method, the colletotrichum camelliae liquid is taken as an induction factor, the ultrasonic induction technology is utilized to strengthen the absorption of tenebrio molitor to the induction factor, meanwhile, a supercritical / ethanol cosolvent extraction process and a bionic affinity chromatography purification process are combined, the efficient extraction of the antibacterial peptide is realized, and the obtained antibacterial peptide has a relatively good inhibition effect on the colletotrichum camelliae. A tea tree anthracnose resisting leaf fertilizer is further prepared through the tenebrio molitor antibacterial peptide, the leaf fertilizer has a good prevention and treatment effect on tea tree anthracnose caused by camellia colletotrichum spinosus, meanwhile, tea growth can be promoted, and the yield reduction caused by anthracnose and nutrition deficiency of tea tree crops can be effectively reduced.

The invention relates to the technical field of food packaging, in particular to a dual-antioxidant packaging film as well as a preparation method and application thereof. The dual antioxidant packaging film disclosed by the invention is prepared from the following components in parts by mass: 3.2 to 4 parts of starch, 0.8 to 1 part of glycerol, 0.8 to 1 part of polysaccharide, 0.02 to 0.16 part of berberine / anthocyanin self-assembled nanoparticles and 93.98 to 95.2 parts of water. The packaging film prepared by the invention is uniform in structure, has good mechanical properties and moisture and oxygen barrier properties, shows an intelligent slow release characteristic in a pH value change or oxidation environment, can realize controllable release of the tea tree essential oil, and significantly improves the antioxidant and antibacterial activity of the film. The material source is natural, renewable and biodegradable, and the environment-friendly fresh-keeping packaging material is suitable for fresh-keeping packaging of foods such as fruits, vegetables and meat products, and has good environment friendliness and industrial application prospects.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.

A novel autophagy activator is provided. An autophagy activator, a hyaluronic acid production promoter, and an antioxidant, each of which contains a lactic acid fermentation product obtained by fermenting a raw material derived from a tea tree with lactic acid bacteria.

The invention belongs to the crossing field of tea cultivation and photovoltaic agriculture, and particularly relates to a device and method for improving summer tea quality through photovoltaic panels, the device comprises supporting frames and auxiliary frames arranged between the two adjacent supporting frames, and photovoltaic panel assemblies are rotationally arranged in the supporting frames and the interiors of the supporting frames; a plurality of groups of supporting mechanisms are arranged below the supporting frame, a supporting frame is arranged between every two adjacent groups of supporting mechanisms, an angle adjusting mechanism used for driving and supporting is arranged in each supporting frame, the angle adjusting mechanisms are arranged on the lower surface of the photovoltaic panel assembly, a transmission connecting mechanism is arranged on one side of each angle adjusting mechanism, and the transmission connecting mechanism is arranged on the other side of each angle adjusting mechanism. The transmission connection mechanism is used for transmission connection of every two adjacent angle adjusting mechanisms. According to the device, the device is arranged at intervals or continuously, meanwhile, the effect of efficiently adjusting the angle is achieved, and by dynamically adjusting the angle and the light transmittance of the photovoltaic panel, light-suitable growth of tea trees is achieved.

The invention relates to the technical field of treatment of eczema-like skin symptoms, in particular to an essential oil composition for treating eczema-like skin symptoms and application thereof, and the essential oil composition comprises the following components in parts by weight: 10-20 parts of lavender essential oil, 5-10 parts of tea tree essential oil, 30-40 parts of olive oil, 5-10 parts of squalane, 0.5-1 part of vitamin E and 1-5 parts of plant extract. The lavender essential oil and the tea tree essential oil are compounded, and the olive oil, the squalane, the vitamin E and the plant extract are supplemented, so that symptoms such as skin erythema, papule and pruritus caused by eczema can be effectively relieved, and good anti-inflammatory, antibacterial, moisturizing and repairing effects are achieved. The composition has the advantages of simple components, simplified preparation process, low production cost and wide application range, and can better meet clinical requirements.

The invention relates to the technical field of tea fruit green tea processing, in particular to a tea fruit green tea processing method which comprises the following steps: S1, picking immature tea fruits from April to July, removing unqualified fruits such as damaged fruits and fruits damaged by worms, and then cleaning; s2, spreading the tea fruits, and performing indoor withering until the weight loss ratio of the tea fruits is 20-30%; step S3, carrying out fixation on the withered tea fruits, namely carrying out primary fixation (steaming) on the tea fruits, and then carrying out secondary fixation (baking) while the tea fruits are hot, so that the tea fruits are uniform in fixation, dark green in appearance and soft in hand pinching. According to the invention, a set of processing technical route of green tea adapting to the physical characteristics of immature tea fruits is constructed around the tea tree by-product which is neglected for a long time, high-value utilization of tea fruit resources and development of low-caffeine tea drinks are realized under the condition of not increasing equipment investment, and the method has remarkable practical value and popularization and application prospects.

This invention relates to the field of sightseeing tea garden landscape technology, specifically a structure for creating a sightseeing tea garden landscape. The structure includes tea trees, planting areas, partitions and guide plates, roads, auxiliary sightseeing and maintenance units, and partition walls. The planting areas are evenly and symmetrically distributed, roads are located between the planting areas, and partitions and guide plates are located at the edges of the planting areas. The tea trees are evenly distributed within the planting areas, and the partition walls are located around the perimeter of the planting areas. The auxiliary sightseeing and maintenance units are located on the planting areas. This invention, by setting auxiliary sightseeing and maintenance units in each planting area, utilizes the viewing section within these units to achieve automatic detection and irrigation of the tea trees, as well as to provide different colors of light, making the tea garden visually appealing. Furthermore, the viewing section can automatically move back and forth, causing the light to oscillate, greatly enhancing the visual appeal.

The present application relates to a kind of compositions containing potato extract and with extended satiety and its preparation method and application, and the composition includes the following weight parts of raw materials: plant extract 30-40 parts, protein component 10-20 parts, dietary fiber component 30-40 parts and auxiliary materials 5-10 parts;Wherein, the plant extract includes potato extract, white kidney bean extract, tea flower extract and lotus leaf extract, the protein component is at least one in pea protein, soybean protein and oat protein, the dietary fiber component is the combination of inulin, resistant dextrin, psyllium husk powder, apple fiber powder and citrus fiber powder.The composition of the present application has significant advantages in controlling body weight and managing satiety, and realizes the weight loss effect of "reducing heat absorption, extending satiety and promoting metabolism" in multiple dimensions, forms sustainable metabolic health support long-term value, and can be used for preparing weight loss body shaping functional food.

This utility model belongs to the field of tea tree cultivation technology, and particularly relates to a tea tree seedling cultivation device, including a support plate, a middle partition plate installed at the top of the support plate, a top frame plate installed at the top of the middle partition plate, and multiple sets of mounting slots evenly spaced on the top frame plate. Multiple sets of drive discs are rotatably connected to the middle partition plate, and each drive disc has a linkage gear set installed at its bottom. By installing a rotatable connecting bracket at the top of the top frame plate, and mounting multiple sets of supplementary lighting lamps on the connecting bracket, when the illumination angle of the supplementary lighting lamps needs to be adjusted, a third motor drives the drive gear to rotate, which in turn drives the third motor on the connecting bracket to rotate, thereby automatically adjusting the illumination angle of the supplementary lighting lamps. This solves the defect of fixed illumination angle in existing technologies. Through synchronously rotating mounting cylinders and automatic adjustment of the supplementary lighting lamp angle, this device effectively adapts to the light requirements of tea tree seedlings at different growth stages, improving photosynthetic efficiency.

The invention relates to a tea tree nitrogen nutrition diagnosis method based on unmanned aerial vehicle multi-source remote sensing. The method comprises the following steps: S1, setting a gradient nitrogen application experiment; s2, collecting tea tree shoot samples, and measuring biomass and nitrogen content data of overground parts; collecting and preprocessing tea tree canopy remote sensing data; s3, constructing a critical nitrogen dilution curve, and calculating to obtain a nitrogen nutrition index NNI; s4, constructing a multi-source fusion feature set; s5, performing feature optimization selection on the multi-source remote sensing fusion feature set; s6, taking the optimal subset in S5 as input, taking the NNI in S3 as output, training an NNI estimation model by utilizing a machine learning algorithm, and selecting and determining an optimal estimation model through model precision; s7, performing inversion and spatial mapping on the tea garden NNI, and performing nitrogen nutrition status diagnosis according to the NNI value; and S8, topdressing the nitrogen fertilizer in a flat cabinet for the diagnosed nitrogen-deficient area. The method can realize large-area, lossless and rapid diagnosis of the nitrogen condition of the tea garden, guide variable fertilization, save fertilizer and increase yield, and has remarkable environmental protection benefits.

This invention discloses a tea tree oil-thyme oil-chitosan composite film, its preparation method, and its application, belonging to the field of food preservation film technology. The preparation method of the tea tree oil-thyme oil-chitosan composite film includes the following steps: dissolving polyvinyl alcohol in water to obtain a polyvinyl alcohol solution; adding glacial acetic acid, chitosan, plasticizer, and emulsifier to the polyvinyl alcohol and mixing evenly; then adding tea tree oil and thyme oil, homogenizing and mixing, and degassing to obtain a film-forming liquid; and drying the film-forming liquid to form a film to obtain the tea tree oil-thyme oil-chitosan composite film. The tea tree oil-thyme oil-chitosan composite film prepared by this invention has excellent antibacterial effects and improves the preservation time of sweet cherries.

The application discloses a water and fertilizer integrated tea tree plug seedling device and a seedling method, and steps are as follows: 1, mother tree treatment; 2, preparation of the device: the cutting substrate formula is grass charcoal: loess: residue: perlite; 3, shoot selection: selecting vigorous and pest-free current-year semi-lignified branches from the mother plant in a mother garden; 4, shoot cutting: pruning the collected branches into one growth point and half a leaf; 5, cutting: using a rooting agent; 6, rooting period management: the nutrient solution is a seedling technology matched with the cutting device, the substrate is continuously transported to the tea cutting root, the root is strengthened, and the seedling is strengthened; 7, seedling cultivation: the tea rooting nutrient solution is used in cooperation with the seedling device. The application also relates to a device, and capillary tubes are connected with the plug and a water storage device respectively. The water and fertilizer integrated management in the tea cutting seedling process is realized, the labor cost can be saved, the survival rate of tea is improved, and the application has application value.

The application discloses a kind of biological base alcohol type beauty glue and preparation method thereof, it is related to adhesive technical field.The raw material composition of the beauty glue includes biological base polysiloxane, nano zinc oxide, biological base composite mildew inhibitor, hydrophobic fumed silica, titanium acid tetraisopropyl ester, methyl tributyl ketone oxime base silane, nano calcium carbonate, epoxy soybean oil, tea polyphenol;The biological base polysiloxane: raw material includes biological base 1,3-propylene glycol, hydroxyl end-capped polydimethylsiloxane, silane coupling agent;The biological base composite mildew inhibitor: raw material includes lecithin, epsilon-polylysine, tea tree oil, vitamin E.The preparation method of beauty glue includes preparing biological base polysiloxane, preparing biological base composite mildew inhibitor, basic filler dispersion, and preparing beauty glue finished product step.The biological base alcohol type beauty glue prepared in the application has good mildew-proof effect and excellent mechanical properties.

The application provides a tea tree waste recycling method, which processes tea tree waste into a film for tea tree planting, or processes tea tree waste into a bio-fertilizer for tea tree planting, and the tea tree waste includes tea tree branches, tea leaves, tea dregs and tea stems. The method provided by the application performs special primary processing on the tea tree waste, and does not introduce too many chemical components and pesticides. Meanwhile, the method processes from soil to film combination, and has a 1+1>2 effect.

This utility model discloses a device for suppressing weeds in tea gardens, including tea tree planting strips, shading strips, and a set of symmetrically arranged shading components. The shading strips are arranged between the tea tree planting strips, forming strip-shaped areas that are parallel to and alternate with the tea tree planting strips. The shading components include a support frame and a holding frame. The bottom of the support frame is fixed in the soil by anchor rods, and the top of the support frame is connected to the holding frame by hinges. The top surface of the holding frame is covered with a shading net. This utility model uses shading components to cover the weed growth area, thereby controlling light and blocking the photosynthesis of weeds to suppress weed growth. At the same time, the shading strips are used to further suppress weeds. The structural configuration of the shading strips suppresses the growth of weeds in the area below and adjacent to them by physically blocking light.

The invention discloses a private care wet tissue containing a bacteriostatic composition, the private care wet tissue comprises a composite non-woven fabric base material and an impregnation liquid, the impregnation liquid comprises the bacteriostatic composition, a soothing anti-inflammatory agent, a moisturizing repairing agent, an odor removal regulator, a cosolvent and a stabilizer; the preparation method comprises the following steps: adding purified water, a citric acid-sodium citrate buffer agent and EDTA (Ethylene Diamine Tetraacetic Acid) disodium into a vacuum stirring tank, then adding glycerol, micromolecular sodium hyaluronate and ceramide 3, and then adding L-lactic acid and a lactobacillus acidophilus fermentation product; sequentially adding nisin, cranberry extract, polyhexamethylene biguanide hydrochloride and beta-cyclodextrin inclusion tea tree essential oil, and finally adding dipotassium glycyrrhizinate, allantoin and citrus extract. Different from the prior art that private care wet tissues only have a single antibacterial function and neglect flora balance, peculiar smell is covered by depending on essence, the base material and the steeping liquor are designed at the same time, and the functions of bacteriostasis and antibiosis, gentle private care, odor removal and itching relieving, inflammation prevention and swelling prevention, flora balance maintenance and the like are achieved.

The invention belongs to the technical field of tea tree cultivation, and particularly relates to a tea tree cultivation method for improving summer tea leaf quality and tea leaves. The technical problem to be solved by the invention is that the quality of tea trees is reduced in summer. According to the technical scheme, the method comprises the steps that after spring tea production is finished, tea tree canopies are pruned; building a shading shed above the trimmed tea trees, and covering the trimmed tea trees with a shading net; a spectrum selective light film is laid above the sunshade net in the tea row direction, so that a stable shading light environment is provided for tea trees before summer tea is picked. The tea is prepared from one bud and two leaves of the tea tree cultivated by the method, the main purpose of the invention is to provide a new choice for the tea tree cultivation method for improving the quality of the summer tea and the tea, and a new technical path is provided for directional quality improvement and high-value utilization of the summer tea.

The invention discloses a method for detecting subcellular localization of a functional gene by using a tea tree leaf, a target gene is expressed in the tea tree leaf through an agrobacterium transformation method, the method has the advantages of short period, rapidness, high conversion rate, low cost, easiness in operation and the like, and the localization condition of the target gene in the tea tree leaf can be more intuitively and accurately observed; and important technical support is provided for tea tree gene function research. Compared with mode plants such as tobacco and arabidopsis thaliana, the novel subcellular localization experimental material has the advantages that the tea tree leaves are used for observing subcellular localization, and a more intuitive and more accurate mode is provided for gene function research in tea trees. In addition, the method is short in conversion period, fluorescence signals can be observed 36-48 hours after conversion, and the method is fast and efficient.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for auxiliary selection of a high-ester catechin tea tree and application thereof, and belongs to the technical field of molecular markers. The SNP molecular marker combination provided by the invention comprises three sites, namely an SNP1 (Single Nucleotide Polymorphism) located at Chr2: 197057556, an SNP2 located at Chr4: 36953566 and an SNP3 located at Chr11: 115194214; by detecting the genotypes of the SNP loci in a tea tree sample, a tea tree single plant with high ester catechin (CG, GCG and EGCG) content can be predicted and screened early, quickly and accurately, the breeding efficiency of a tea tree variety with high ester catechin content is remarkably improved, and the method has an excellent application prospect.

The invention belongs to the technical field of tea tree gene application, and particularly relates to application of a tea tree CsPHT gene in improving tea geometrid and colletotrichum gloeosporioides resistance of a tea tree. The invention discloses a tea tree CsPHT gene, the nucleotide sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.1, the tea tree CsPHT gene codes 434 amino acid residues, and the amino acid sequence of the tea tree CsPHT gene is as shown in SEQ ID NO.2. The CsPHT gene is proved to be capable of catalyzing biosynthesis of tea tree defense related compounds such as feruloyl putrescine, coumaroyl putrescine and cinnamoyl putrescine for the first time, and the conservation of the catalytic function of the CsPHT gene is proved through heterologous expression. An in-vivo instantaneous function verification result of a tea tree shows that the over-expression of the CsPHT gene can significantly improve the resistance of the tea tree to tea geometrid and tea anthracnose bacteria. The CsPHT gene is proved to be a key positive regulation factor for regulating the resistance of the tea tree to the tea geometrid and the tea anthracnose fungus. The invention provides important gene resources and molecular targets for cultivating new varieties of high-resistance and multi-resistance tea trees.