The invention relates to a natural green bio-organic pesticide composition and its application. The technology is characterized in that: A, natural camphor, rheum officinale, Chinese cassia tree, eucalyptus oil, dry ginger, common fennel fruit, capsicum and auxiliary materials are made into a natural biological pesticide; and B, rhizoma atractylodis, magnolia cortex (prepared with ginger), Poria cocos, raw Pinellia tuber, oil of patchouli,dried orange peel, dahurian angelica root, areca peel, extractum glycyrrhizae, perilla leaf oil and auxiliary materials are made into a natural biological pesticide. The pesticide provided by the invention can be directly applied to melon, fruit, leaf, vegetable legumes, tea, traditional Chinese medicines, flower, rape, cotton, tobacco and the like for the control of insect diseases. After applied with the biological pesticide, vegetables and fruits can be directly eaten without residual pesticide harm to human body.