443 results about "Biopesticide" patented technology

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.

The invention relates to a brand-new microbial natural metabolite, namely Fcl-8, which is derived from a fermentation solution of Xenorhabdius budapestensis XBD8, and is characterized in that the Fcl-8 is a new microbial natural metabolite, namely Fcl-8, which is derived from the fermentation solution of the Xenorhabdius budapestensis XBD8. The purified Fcl-8 is a snow white flocculent crystal and is very easy to dissolve in water, and the solid state of the purified Fcl-8 is as shown in Figure 1. The chemical structural formula of the Fcl-8 is as shown in a figure 2, the chemical formula is C57H102N14O12, the molecular weight is 1175.78744, and the typical secondary mass spectrum information of the Fcl-8 is as shown in a figure 3. The Fcl-8 is derived from a microbial natural metabolite, is novel in structure and high in biological activity, and has very high potential to be developed into a novel biopesticide.

The invention discloses a semi-solid-state complex no-tillage dry direct seeding method for crop seeds. The no-tillage dry direct seeding method comprises the following steps: S1, keeping a water layer of 1cm-5cm in a ditch, and paving straws obtained by harvesting preceding crops on a ditch surface; S2, applying a base fertilizer on the ditch surface paved with the straws and cleaning the ditch; S3, seeding the crop seeds on the ditch surface, and mixing the crop seeds with semi-solid-state biomass materials, a compound fertilizer and a biopesticide before seeding before seeding seeds, wherein the mixing mass ratio is that the crop seeds to the semi-solid-state biomass materials to the biopesticide is (1-10) to (70-800) to (1-3), and the moisture content of the semi-solid-state biomass materials is 40%-80%; and S4, after seeding the seeds, keeping the ditch surface at a dry state for 3-7 days. According to the semi-solid-state complex no-tillage dry direct seeding method, tillage and combustion of winter crop straws are not carried out, a whole land of field immersing water is not used for irrigation and the water immerses after the seeding, and the biological benefits are obvious; the working energy consumption is reduced, stubbles are not killed by pesticides, haze is reduced and the irrigation water is reduced.

The invention discloses a camellia oleifera forest planting ecology digital monitoring management system based on the Internet of Things, and relates to the technical field of planting ecology supervision. Dynamic ecological grid data is combined with an Internet of Things sensor and unmanned aerial vehicle patrol data, a blank area is filled through a spatial interpolation technology, and a real-time camellia oleifera forest ecological monitoring system is formed; dynamically updating a disease and pest risk distribution map through a disease and pest diffusion model and unmanned aerial vehicle patrol data, and identifying a high-risk area in combination with diffusion path simulation; the unmanned aerial vehicle is linked to perform quantitative spraying of biopesticide, prevention and control operation is performed only in a high-risk area, the pesticide usage amount is reduced, and soil microbial communities and an ecological system are protected; through nutrient diffusion and permeation simulation, the influence of a fertilization scheme on soil nutrient loss is evaluated, the fertilization amount and fertilization time are optimized, nutrient waste and water pollution risks are reduced, the irrigation amount and strategy are dynamically adjusted, the water resource utilization efficiency is optimized, and excessive irrigation or drought pressure is avoided.

The invention discloses a preparation method and application of a sophocarpidine nano-pesticide, dendritic mesoporous silica nanoparticles are ultrasonically dispersed in a methanol solution of sophocarpidine, the mixture is stirred for 45-50 h in a dark place at 10-30 DEG C for a reaction, after the reaction, precipitates are centrifugally collected, washed and freeze-dried, and the sophocarpidine nano-biopesticide is obtained. The sophocarpidine nano biopesticide is prepared by taking dendritic mesoporous silica nanoparticles as a carrier, and dendritic mesoporous silica is of a dendritic mesoporous structure and has the characteristics of larger porosity, uniform particle size, high drug loading rate, slow control effect and the like. The dendritic mesoporous silica has the advantages that the dendritic mesoporous silica can be used for preventing and treating trunk-boring, basal and root-eating pests, the matrine as a biological pesticide can avoid the problems of photolysis and the like after being loaded into the dendritic mesoporous silica, the prevention and treatment effect is improved, the drug resistance of the pests is reduced, the goals of reduction and synergism are achieved, and a new thought is provided for sustainable prevention and treatment.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a microbial agent as well as a preparation method and application thereof. The microbial agent comprises an artificial strain, namely a bacillus velezensis P43-DegQ strain, which is obtained by carrying out genetic engineering oriented modification on bacillus velezensis XY40-1 serving as an engineering bacterium. The method comprises the following steps: replacing an original promoter of a DegQ gene in bacillus velezensis XY40-1 with a P43 promoter, connecting the DegQ gene in the XY40-1 with a PBE-P43 vector to construct a P43-DegQ recombinant expression vector, and introducing the P43-DegQ recombinant expression vector into the bacillus velezensis to obtain a bacillus velezensis P43-DegQ strain. According to the invention, the yield of the bacillus velezensis abinogen is improved through genetic engineering oriented modification, so that the microbial control effect of a microbial agent containing the strain is improved. A resonance biopesticide suspending agent and a resonance microbial seed coating agent prepared from the microbial agent are subjected to resonance treatment, the effects of plant epidemic disease prevention and control and seed germination are greatly improved compared with the prior art, and the microbial agent has a very strong application prospect.

The invention discloses Pseudomonas aeruginosa KS1-2 and application thereof, the strain is separated from a soil sample in a rice field and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.33996. The invention further discloses a method for preparing the Pseudomonas aeruginosa KS1-2. The Pseudomonas aeruginosa KS1-2 has the advantages that the Pseudomonas aeruginosa KS1-2 can be used for preparing the Pseudomonas aeruginosa. The pseudomonas aeruginosa KS1-2 has a remarkable inhibition effect on a plurality of rice pathogenic bacteria; the growth of various plant pathogenic fungi can be effectively inhibited, and the broad-spectrum antibacterial property is achieved; the strain and the microbial inoculum can be prepared into a biocontrol agent for preventing and treating plant pathogens, can reduce plant diseases, are green and environment-friendly, have good application potential, and lay a foundation for research and development of multifunctional biocontrol microbial inoculum and biopesticide.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses an agricultural synergist containing astragalus polysaccharide and application of the agricultural synergist, and belongs to the technical field of killing agents for biopesticides. The synergist comprises astragalus polysaccharide, a polysorbate / lecithin compound emulsifier, a carboxymethyl chitosan / siloxane penetrant and a modified accelerant synthesized by palladium catalysis. Polysaccharide is structurally modified through a blue light LED induced tetra (triphenylphosphine) palladium catalytic reaction, and the epidermal penetration efficiency of the biopesticide on tetranychus urticae is improved by 3.2 times in combination with the synergistic permeation effect of a siloxane derivative. The mite killing rate of the suspending agent prepared according to a preferable formula reaches 92.5% within 24 hours, and the lasting period is prolonged to 14 days. The system overcomes the defects of slow effectiveness and poor stability of the traditional biopesticide, by means of a targeted combination mechanism of natural components, the environment friendliness is kept while the original pesticide dosage is reduced by 50%, and the system is particularly suitable for the pest control requirement of organic agriculture.

The invention belongs to the technical field of phytochemistry, and particularly relates to a benzo [e] pyrrolo [1, 2-a] aza # imgabs0 # compound as well as a preparation method and application thereof. The preparation method comprises the following steps: fermenting cigar stem barks, drying the fermented cigar stem barks in the sun, crushing and sieving to obtain a material a; adding a first solvent into the material a for reflux extraction to obtain an extracting solution, concentrating the extracting solution, adding a second solvent for extraction, combining the extracted extraction phases, and performing vacuum concentration to obtain an extract b; carrying out silica gel column chromatography on the extract b, eluting with chloroform-acetone eluent, monitoring by TLC (Thin Layer Chromatography), and collecting the eluent; and purifying the eluent by using a sephadex column, and separating and purifying by using high performance liquid chromatography to obtain the benzo [e] pyrrolo [1, 2-a] aza # imgabs 1 # compound with tobacco black shank resisting activity. The compound is novel in structure, has relatively good black shank resistance activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The invention provides a bactrocera minax forked head transcription factor FOXO as well as a coding gene and application thereof, and belongs to the technical field of biological prevention and control of crop pests. A nucleic acid molecule of the citrus fruit fly forked head transcription factor FOXO is cloned, dsRNA for interfering the nucleic acid molecule of the citrus fruit fly coded forked head transcription factor FOXO is synthesized, and the dsRNA is introduced into the citrus fruit fly by using an injection method and a feeding method to carry out RNAi on the citrus fruit fly forked head transcription factor gene FOXO. After the dsRNA is injected or fed to the bactrocera minax, the survival rate, the growth rate, the weight and the overall expressive force of the bactrocera minax are remarkably reduced, and it is indicated that the fork head transcription factor FOXO of the bactrocera minax plays an important role in the growth and development process of insects. The invention provides a new method for molecular regulation and control of pests, and also provides a theoretical basis for deeply understanding the growth and development mechanism of insects and creating a new biopesticide preparation.

The invention relates to the technical field of biopesticides, in particular to a compound preparation for preventing and treating meloidogyne incognita as well as a preparation method and application of the compound preparation. The compound preparation for preventing and treating meloidogyne pinnatifida comprises the following raw materials in percentage by mass: 0.1-5% of azadirachtin or compound azadirachtin, 30-60% of castor seed meal, 35-65% of activated carbon and the balance of an adhesive. The active carbon, the azadirachtin and the castor seed meal are compounded, and the azadirachtin realizes efficient contact killing by inhibiting nematode respiratory chains and neural signal transmission and has no drug resistance; the active carbon is used as a slow-release carrier to prolong the pesticide effect (lasting for more than 90 days), and can adsorb soil toxins to improve the microenvironment; the castor seed meal can provide organic matters to promote the proliferation of soil probiotics and improve the soil structure on one hand, and is rich in ricin on the other hand, so that the hatching of nematode eggs can be effectively inhibited; the three components are combined to realize synergistic interaction through three mechanisms of slow release, killing and improvement.

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention discloses a rice endophytic Wille kamplaromyces cancerasus GLARF-10, a biological preparation thereof and application of the rice endophytic Wille kamplaromyces cancerasus GLARF-10 in preventing and treating rice blast, and belongs to the technical field of biopesticides. According to the present invention, the Wille kamplaromyces cerevisiae GLARF-10 is preserved in Guangdong Microbial Culture Collection Center on December 31, 2024, and the preservation number of the Wille kamplaromyces cerevisiae GLARF-10 is GDMCC No: 65695. The fermentation liquor or the supernate of the deliciosus velava GLARF-10 is used for preparing a corresponding biological agent; the cyanobacteria velava GLARF-10 and the biological preparation of the cyanobacteria velava GLARF-10 are applied to prevention and treatment of rice blast. The bacillus subtilis is mainly used for inhibiting normal growth of magnaporthe oryzae and has an excellent potential biocontrol effect of preventing and treating the magnaporthe oryzae, fermentation liquor or supernate of the bacillus subtilis is sprayed to rice tissues by controlling the biomass of the bacillus subtilis to be used for preventing and treating the magnaporthe oryzae, and the influence on the ecological environment is small; the strain is low in culture condition requirement and has good development and application prospects.

The present invention relates to a biopesticidal composition comprising combination of elemental sulphur and at least one biochemical pesticide selected from thymol and eugenol or their derivatives. The invention particularly relates to a biopesticidal composition comprising elemental sulphur in the range of 1% w / w to 90% w / w of the total composition; at least one biochemical pesticide in the range of 0.1% w / w to 30% w / w of the total composition wherein the biochemical pesticide is selected from thymol and eugenol or their derivatives; and at least one agriculturally acceptable excipient in the range of 5% w / w to 98% w / w of the total composition. The composition has particles in the size range of 0.1 micron to 50 microns. The present invention also relates to a method for protecting the plant against pests or diseases by treatment of plant, plant propagation material, locus, parts thereof or the surrounding soil with a biopesticidal composition comprising of an effective amount of elemental sulphur; an effective amount of at least one biochemical pesticide selected from thymol and eugenol or their derivatives and at least one agriculturally acceptable excipient.

The invention discloses a mollusk attractant as well as a preparation method and application thereof in snail prevention and control, and belongs to the technical field of biopesticides, temperature-responsive microcrystalline cellulose particles are used as a main component of a coating premix, and an attractant base material containing active components, essence, amino acid, molasses and flour is coated, so that the mollusk attractant is obtained. The attractant has temperature responsiveness and can meet the field temperature of snails in spring and autumn high-incidence periods in agricultural production, the essence and the molasses are main components with an inducing function, snails can be better trapped, and the snails are not prone to loss due to the influence of the complex environment in a farmland; the temperature-responsive microcrystalline cellulose particles disclosed by the invention have amphipathy, the hydrophilicity can realize rapid spreading of the attractant in a farmland, and the hydrophobicity can realize that the attractant is not easily washed by water flow and rainwater in the farmland, so that the service life of the attractant is prolonged.

The invention discloses an Inquilinus strain and application thereof, the Inquilinus strain is # imgabs0 # and is preserved in the China Center for Type Culture Collection (CCTCC) on January 6, 2025, and the preservation number of the Inquilinus strain is CCTCC NO: M 2025044. The Inquilinus strain is subjected to activation and seed culture to obtain the microbial agent. The Inquilinus strain and the microbial agent can be used as biopesticides, can improve the prodenia litura resistance of cotton, and can be used for preventing and controlling tomato late blight and virus diseases, tomato brown wrinkled fruit virus diseases and tomato chlorosis viruses. The method has the advantages of low production cost, good prevention and control effect, high efficiency, no toxicity, environmental protection and the like.

The invention discloses bacillus velezensis, a microbial inoculum and application of the bacillus velezensis in prevention and treatment of soybean gray speck disease. The bacillus velezensis YD21 has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.3334 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The bacillus velezensis YD21 provided by the invention is from seeds of wild soybeans LYD120068, can be used for effectively antagonizing 16 plant pathogenic fungi such as soybean gray leaf spot germs, corn black bundle germs, apple core germs, corn gray leaf spot germs and the like, and has an obvious prevention and treatment effect on the soybean gray leaf spot disease, so that the bacillus velezensis YD21 can be used for preventing and treating the soybean gray leaf spot disease, and the bacillus velezensis YD21 can be used for preventing and treating the soybean gray leaf spot disease. The method has a wide application prospect in the development of biological pesticides and biological organic fertilizers.

The invention discloses bacillus safensis and application thereof in prevention and control of pine wood nematode diseases, and belongs to the technical field of biocontrol of plant diseases. The invention discloses bacillus safensis GDWS1 which is separated from resistant masson pine tissues and has a relatively good inhibition effect on pine wood nematode diseases, and the preservation number of the bacillus safensis GDWS1 is GDMCC No: 66264. The strain GDWS1 has high lethal activity on pine wood nematodes, the correction death rate of the strain GDWS1 on the pine wood nematodes reaches 90% or above, and active substances capable of poisoning and killing the pine wood nematodes and good in stability can be generated; the requirements on culture conditions are low, and the ecological environment is friendly and pollution-free. A greenhouse control experiment is carried out by using pinus massoniana seedlings, the strain GDWS1 can effectively reduce the disease index of the pinus massoniana seedlings inoculated with pine wood nematodes, the control effect reaches 45.88%, and the strain has a good application prospect in prevention and control of pine wood nematode diseases, provides a new biocontrol bacterium type for biological prevention and control of pine wood nematode diseases, and has the potential of being developed into biopesticides.

The application discloses a production method of a high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and belongs to the technical field of biological pesticide production. 8 The virus yield of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus is 6*10 The virus loss rate is reduced by 1.4% by adding 0.1% detergent before centrifugal purification of the virus, and the high yield of the virus is further ensured.The insecticidal effect of the high-efficiency strain produced by the method is increased by more than 64.1% than that of an ordinary strain, and the high-efficiency property is continuously maintained.The method has a very beneficial effect on the production of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and provides a solid guarantee for popularization and application of the high-efficiency strain.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The utility model provides biopesticide production equipment with an anti-precipitation function, which relates to the technical field of biopesticides and comprises a tank body, a first rotating shaft, a second rotating shaft and a third rotating shaft are rotatably connected in the tank body, and a first gear and a second gear are coaxially and fixedly connected to the peripheries of the first rotating shaft and the second rotating shaft respectively. According to the utility model, on one hand, the plurality of belt pulleys are arranged outside the tank body, and the belt pulleys are connected with the belt, so that the crushing cutters, the crushing rollers and the stirring rods can be linked, the linkage among all parts is increased, and a plurality of structures are driven by one motor; secondary crushing of the raw materials is achieved, the solubility and the release speed of active ingredients are improved, the medicine effect is enhanced, the working efficiency is greatly improved, and the user experience is improved.