298 results about "Biopesticide" patented technology

The invention belongs to the technical field of biological control, and particularly relates to a pseudomonas flax DY-31 strain, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the pseudomonas lini (Pseudomonas lini) DY-31 is CGMCC (China General Microbiological Culture Collection Center) No.34088. The pseudomonas lini (Pseudomonas lini) DY-31 is The pseudomonas flax DY-31 or the biocontrol inoculant thereof is applied to prevention and treatment of plant soil-borne diseases, specifically including peanut black rot, peanut southern blight, peanut sclerotinia rot, peanut leaf rot sclerotinia rot, peanut basal rot and wheat stem basal rot. The pseudomonas flax DY-31 provided by the invention is an important strain resource, can be used for creating a biopesticide preparation, and is used for green prevention and treatment of soil-borne diseases of peanuts and wheat. In addition, the DY-31 strain also has various growth promoting functions, and has important development and application values in the aspects of agricultural biological control and plant growth promoting.

The invention relates to a brand-new microbial natural metabolite, namely Fcl-8, which is derived from a fermentation solution of Xenorhabdius budapestensis XBD8, and is characterized in that the Fcl-8 is a new microbial natural metabolite, namely Fcl-8, which is derived from the fermentation solution of the Xenorhabdius budapestensis XBD8. The purified Fcl-8 is a snow white flocculent crystal and is very easy to dissolve in water, and the solid state of the purified Fcl-8 is as shown in Figure 1. The chemical structural formula of the Fcl-8 is as shown in a figure 2, the chemical formula is C57H102N14O12, the molecular weight is 1175.78744, and the typical secondary mass spectrum information of the Fcl-8 is as shown in a figure 3. The Fcl-8 is derived from a microbial natural metabolite, is novel in structure and high in biological activity, and has very high potential to be developed into a novel biopesticide.

The invention discloses a semi-solid-state complex no-tillage dry direct seeding method for crop seeds. The no-tillage dry direct seeding method comprises the following steps: S1, keeping a water layer of 1cm-5cm in a ditch, and paving straws obtained by harvesting preceding crops on a ditch surface; S2, applying a base fertilizer on the ditch surface paved with the straws and cleaning the ditch; S3, seeding the crop seeds on the ditch surface, and mixing the crop seeds with semi-solid-state biomass materials, a compound fertilizer and a biopesticide before seeding before seeding seeds, wherein the mixing mass ratio is that the crop seeds to the semi-solid-state biomass materials to the biopesticide is (1-10) to (70-800) to (1-3), and the moisture content of the semi-solid-state biomass materials is 40%-80%; and S4, after seeding the seeds, keeping the ditch surface at a dry state for 3-7 days. According to the semi-solid-state complex no-tillage dry direct seeding method, tillage and combustion of winter crop straws are not carried out, a whole land of field immersing water is not used for irrigation and the water immerses after the seeding, and the biological benefits are obvious; the working energy consumption is reduced, stubbles are not killed by pesticides, haze is reduced and the irrigation water is reduced.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention belongs to the technical field of prevention and treatment of rice blast, and particularly relates to a polypeptide MP1 for inhibiting rice blast bacteria and application thereof. The invention discloses a polypeptide MP1 for inhibiting magnaporthe oryzae. The amino acid sequence of the polypeptide MP1 is as shown in SEQ ID NO. 1. The polypeptide MP1 has an obvious inhibition effect on the magnaporthe oryzae appressorium, and the EC50 formed by the MP1 polypeptide on the appressorium is 134.60 mu M. Experiments show that the polypeptide can effectively interfere the normal differentiation and development of the appressorium in the key pathogenic stage of magnaporthe oryzae, so that the polypeptide has a strong prevention and control potential in the early stage of pathogenic bacterium infection. The polypeptide MP1 provided by the invention can significantly reduce the pathogenicity of magnaporthe oryzae to barley and rice, and provides an experimental basis for developing a novel biopesticide for preventing and treating the magnaporthe oryzae.

The invention discloses a mollusk attractant as well as a preparation method and application thereof in snail prevention and control, and belongs to the technical field of biopesticides, temperature-responsive microcrystalline cellulose particles are used as a main component of a coating premix, and an attractant base material containing active components, essence, amino acid, molasses and flour is coated, so that the mollusk attractant is obtained. The attractant has temperature responsiveness and can meet the field temperature of snails in spring and autumn high-incidence periods in agricultural production, the essence and the molasses are main components with an inducing function, snails can be better trapped, and the snails are not prone to loss due to the influence of the complex environment in a farmland; the temperature-responsive microcrystalline cellulose particles disclosed by the invention have amphipathy, the hydrophilicity can realize rapid spreading of the attractant in a farmland, and the hydrophobicity can realize that the attractant is not easily washed by water flow and rainwater in the farmland, so that the service life of the attractant is prolonged.

The application discloses a production method of a high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and belongs to the technical field of biological pesticide production. 8 The virus yield of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus is 6*10 The virus loss rate is reduced by 1.4% by adding 0.1% detergent before centrifugal purification of the virus, and the high yield of the virus is further ensured.The insecticidal effect of the high-efficiency strain produced by the method is increased by more than 64.1% than that of an ordinary strain, and the high-efficiency property is continuously maintained.The method has a very beneficial effect on the production of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and provides a solid guarantee for popularization and application of the high-efficiency strain.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The utility model relates to the technical field of pesticide mixing, and discloses a biopesticide mixing device which comprises a feeding mechanism and a mixing mechanism, the feeding mechanism comprises a left fixing plate and a right fixing plate, a mounting frame is fixedly mounted between the tops of the two fixing plates, anti-roll rods are fixedly mounted between the tops of the fixing plates and the mounting frame, and the anti-roll rods are fixedly connected with the mixing mechanism. Two moving wheels are fixedly mounted at the bottom of each fixing plate, the mixing mechanism comprises a mixing tank arranged below the mounting frame, a stirring motor is fixedly mounted in the middle of the top of the mixing tank, a stirring rod is fixedly mounted on an output shaft at the bottom of the stirring motor, and the stirring rod is arranged in the mixing tank. And four stirring fan blades are fixedly mounted on the surface of the outer wall of the stirring rod. According to the biopesticide mixing device, different types of biopesticide raw materials can be conveniently stored and taken at any time through the four fixing frames fixed in the middle of the top of the mounting frame and the storage tanks connected with the fixing frames in an inserted mode.

The application discloses a low-temperature storage system for biological pesticides, which comprises a storage box body, a switch door arranged at the lower end of the storage box body, a storage mechanism arranged in the storage box body and used for supporting a pesticide bottle tray, and a transfer mechanism arranged at the left side of the storage mechanism and used for transferring the pesticide bottle to the supporting mechanism; when the pesticide bottles are stored, the switch door is opened, the transfer mechanism takes the tray filled with the pesticide bottles and places the tray into the storage mechanism, automatic placement of the pesticide bottles is realized, labor intensity is reduced, manual intervention is reduced, and bacterial pollution is prevented.

The invention relates to the technical field of biopesticides, in particular to toxin bait and a preparation method thereof, and the toxin bait comprises the following components in parts by weight: 30-35 parts of core-shell structure microspheres; 45 to 60 parts of a matrix; 5-8 parts of an adhesive; 3-5 parts of a water-retaining agent; and 1-2 parts of a synergist. According to the toxin bait, through collaborative design of multi-level functional components, the problem of palatability fluctuation caused by dependence on a single plant material in the prior art is remarkably solved, the core-shell structure microspheres serve as a core functional unit, trehalose-gelatin composite gel is used for protecting toxin activity, an outer acrylic resin coating provides an environment barrier, and the toxin bait has the advantages of being high in palatability and high in palatability. A dual protection mechanism is formed, and the long-acting toxicity is improved.

The utility model relates to the technical fields of filling device, and particularly relates to a biological pesticide fermentation liquor filling device, which avoids the frequent start-stop condition of the conveying belt, simultaneously improves the close effect between the connecting pipe and the packaging bottle, and reduces the leakage during the filling of the biological pesticide fermentation liquor, and comprises a base and a conveying belt, the conveying belt is installed at the top end of the base, further comprises a discharging device, a limiting device, a conveying pipe, a connecting pipe and a sealing ring, the top end of the conveying pipe is communicated with the discharging device, the discharging device is used for controlling the discharge of the biological pesticide fermentation liquor, the upper portion of the connecting pipe extends into the conveying pipe, the sealing ring is installed on the outer side wall of the connecting pipe, the sealing ring is slidingly installed in the conveying pipe, the limiting device is arranged on the conveying pipe and the connecting pipe, and the limiting device is used for limiting the filling bottle.

The invention relates to the technical field of pesticides, and particularly discloses a biopesticide containing kasugamycin and application of the biopesticide containing kasugamycin. The preparation method comprises the following steps: obtaining a seed solution, fermenting, acidifying, adsorbing and separating, concentrating and crystallizing, and grinding kasugamycin, an auxiliary agent and water in a mass ratio of (3-4): (1-1.5): (4.5-6) to obtain the biological pesticide containing kasugamycin. The biopesticide containing kasugamycin provided by the invention is environment-friendly and safe, accurate in target and not easy to generate drug resistance, and can meet green agricultural requirements.

The invention discloses a polyketone compound japondiene A as well as a preparation method and application thereof, and belongs to the technical field of microbial metabolites. According to the present invention, the Aspergillus japonicus TE-739D is found, the polyketone compound japondiene A is successfully separated, extracted and prepared from the metabolic fermentation product of the Aspergillus japonicus TE-739D, and through the SciFindern database retrieval and compared with the currently reported compound, the polyketone compound japondiene A is the new structure compound reported for the first time, and can be used for preparing the new structure compound. Functional studies find that the polyketone compound japondiene A has insecticidal activity on spodoptera frugiperda, and technical support is provided for further development of biopesticides.

The environment-friendly biopesticide suspending agent preparation device comprises a stirring barrel, a motor is fixedly connected to the inner bottom face of the stirring barrel, a supporting rod is fixedly connected to the output end of the motor, a mounting block is fixedly connected to the upper surface of the supporting rod, and an electric telescopic rod is fixedly connected to the upper surface of the mounting block; the outer surface of the stirring barrel is fixedly connected with a water tank, the upper surface of the water tank is fixedly connected with a water pump, the output end of the water pump is fixedly connected with a conveying pipe, and the end, away from the water pump, of the conveying pipe is fixedly connected with a rotating spray head; through cooperation of the motor, the supporting rod, the electric telescopic rod, the fixing rod and the adjustable stirring blades, the position of the upper-layer stirring blades can be adjusted according to the height of materials, the optimal stirring depth can be found, it is ensured that materials with different densities and viscosities are fully mixed, and the uniformity of a suspending agent is enhanced.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The utility model discloses a biopesticide feeding device which comprises a movable bottom plate and a material mixing barrel, the material mixing barrel is fixedly installed at the top of the movable bottom plate, a feeding hopper is arranged on the front side of the material mixing barrel, the bottom of the feeding hopper inclines downwards, and a dustproof door is rotationally connected to the front side of the feeding hopper; wherein a dustproof assembly is arranged at the top of the mixing barrel, and a stirring assembly is arranged in an inner cavity of the mixing barrel; the spiral conveyor is rotatably connected to the bottom of the material mixing barrel, a discharging pipe is arranged at the bottom of the material mixing barrel, an electromagnetic valve is installed on the surface of the discharging pipe, and the discharging pipe is communicated with a feeding port of the spiral conveyor through a flexible pipe. According to the utility model, raw materials are mixed through the stirring assembly in the mixing cylinder, and the feeding height of the screw conveyor is adjusted by rotating the screw conveyor, so that the purpose of feeding at different heights is achieved.

The invention discloses p-toluenesulfonic acid 8-tert-butyl diphenyl silyloxy-7-methyl octyl ester as well as a synthesis method and application thereof, and relates to the technical field of biopesticides. The preparation method comprises the following steps: taking 8-bromocaprylic acid as an initial raw material, and firstly reacting with t-BuOK and 18-crown ether-6; then reacting with pivaloyl chloride to generate acid anhydride, and then carrying out acylation reaction with 4-benzyl oxazolidine-2-ketone; then carrying out diastereoselective methylation; then, NaBH4 reduction is carried out; carrying out hydroxyl protection; then carrying out hydroboration oxidation reaction; and then carrying out TsCl sulfonylation, so as to synthesize the p-toluenesulfonic acid-8-tert-butyl diphenyl silyloxy 7-methyl octyl ester. The optical purity of the (S)-p-toluenesulfonic acid 8-tert-butyl diphenyl silyloxy-7-methyl octyl ester synthesized in the invention reaches 98%, and the optical purity of the (R)-p-toluenesulfonic acid 8-tert-butyl diphenyl silyloxy-7-methyl octyl ester synthesized in the invention also reaches 98%.

The application provides a kind of high biocompatibility biological silver nanoparticles, its preparation method, compound microcapsule and application, the biological silver nanoparticles are based on the fermentation broth or fermentation filtrate of biocontrol bacillus B268, the metabolite of B268 is combined on the surface of particle, the antagonistic activity to plant pathogenic bacteria is enhanced, and show high biocompatibility to biocontrol bacteria, biocontrol fungi, plant, model nematode, can be directly embedded with bacteria liquid, the microcapsule prepared fuses the compatible interaction relationship between bacteria, bacterial metabolite, silver nanoparticles, shows synergistic antibacterial effect, and the bacteriostatic activity is higher than that of microcapsule embedded by bacteria liquid or silver nanoparticles alone, and under low temperature preservation condition, microcapsule can keep the stability of bacteria, silver nanoparticles and its synergistic antibacterial activity, provides preparation raw material and preparation method for developing nano biological pesticide.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention discloses a pomacea canaliculata egg wrapping type inactivator and a preparation method thereof, and belongs to the technical field of biopesticides, the pomacea canaliculata egg wrapping type inactivator comprises a component A, a component B and a component C which are physically isolated and independently packaged, the component A is biopolymer matrix liquid and comprises a film-forming framework polymer; the film-forming skeleton polymer is alginate, a composite polymer, weak acid, an endogenous slow-release calcium source and water, and the composite polymer is chitosan; the component B is a gel activating solution and comprises a cross-linking activating agent, water and a modifying agent; the modifier is one of sodium citrate, cellulose nanocrystal or nanoclay; when the component A and the component B are in contact on the surface of an ampullaria gigas egg mass, the component A and the component B are subjected to an in-situ gel reaction to generate a layer of physical barrier gel film; the defects that an existing dehydrating agent scheme is not resistant to rain wash and poor in persistence are overcome, and the core component is derived from biomass and is environmentally friendly.

The invention belongs to the technical field of microbial biopesticides, and particularly relates to a high-efficiency, low-toxicity and environment-friendly microbial pesticide, which is characterized in that effective components of the high-efficiency, low-toxicity and environment-friendly microbial pesticide are formed by compounding microbial active components and compounding factors; wherein the microbial active component is P.megatherium; the microbial active component is P.megatherium; the compound factor is physcion or berberine. The bacterium content of the microbial active component, namely, the preristeria megatherium, is 5.0 * 10 < 10 > cfu / g, and the preristeria megatherium has a relatively good inhibition effect on the growth of fungal pathogenic bacteria mycelia and has the potential of being developed into a microbial bactericide; when the compound is compounded with the existing biopesticide physcion or berberine, the compounded composition has a synergistic effect on the growth of fungal pathogenic bacteria hyphae of crops, and compared with the single use of the microbial active component, the compound composition can improve the prevention and treatment effect on diseases, is beneficial to reducing the application amount of the pesticide, and has a good application prospect. And the method is of great significance to development of compound microbial biopesticides.

The invention discloses a Trichoderma cererum and application thereof, the name of the Trichoderma cererum TR1186A is Trichoderma cererum TR1186A, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.21427. The Trichoderma cererum is named as Trichoderma cererum TR1186A. The Trichoderma cererum is named as Trichoderma cererum TR1186A. The trichoderma cereus provided by the invention is an effective biocontrol strain and can antagonize a tobacco black shank strain, and test results of a plate confrontation test, an indoor pharmacodynamic test, an indoor safety test, a field pharmacodynamic test and the like prove that the strain can be developed into a biological pesticide which is environment-friendly, high in safety and good in control effect; particularly, the bacillus subtilis has a good application prospect in biological prevention and control of tobacco black shank.

This invention belongs to the field of biopesticide technology, specifically relating to a biopesticide for controlling citrus thrips. The biopesticide, used to control citrus thrips, comprises an active ingredient derived from a compound of *Bretschneidera sinensis* powder and methyl salicylate, or a compound of *Bretschneidera sinensis* and ethyl spinosad; wherein the effective bacterial count of the *Bretschneidera sinensis* powder is 2.5 × 10⁻⁶. 8 Spores / g. This invention discovered that *Cladosporium flavum* is effective in killing citrus thrips and can be used as a microbial agent for the control of citrus thrips. Furthermore, this invention combines *Cladosporium flavum* with other existing bioactive ingredients and found that when combined with methyl salicylate or spinosad, it has a synergistic effect on killing citrus thrips. Compared with single-ingredient formulations, it can improve the control effect on citrus thrips, possessing the advantages of being green, safe, and highly efficient, and can provide support for the research and development of biological pesticides for the control of citrus thrips.