26 results about "Citrinin" patented technology

The invention provides a monascus strain ZX-99 capable of expressing multiple beneficial active substances and application of the monascus strain ZX-99, and belongs to the technical field of microorganisms. The preservation number of the monascus strain ZX-99 provided by the invention is CGMCC No.40962, the strain does not produce citrinin (the detection limit is 25 mg / kg), and compared with other monascus cheese and commercially available mould cheese, the prepared cheese sample has the advantages that the content of citrinin in the cheese sample is greatly reduced, and the content of citrinin in the cheese sample is greatly reduced. Substances such as oxymatrine, bufalin, azadirachtin, gamma-aminobutyric acid, hydroxybenzylamine, maleic acid, maltotriose, maltotetraose and diacetyl are enriched in the monascus cheese ZX-99, and the monascus cheese ZX-99 is expected to be used for developing biological functional dairy products or compounding flavor strains.

This invention belongs to the field of microbial fermentation technology and provides a fermentation method for red yeast rice with low citrinin content. Adding gastrodin to the rice fermentation culture medium can effectively reduce the citrinin content in red yeast rice without reducing pigment yield. The method is not only simple to operate and safe and reliable, but also has excellent and stable effects, and is expected to be used in industrial production.

The invention discloses a method for producing burdock functional red yeast rice through solid state fermentation. The monascus ruber NCPS2020003 (the preservation number is CCTCC NO: M 20242830) is provided for the first time, the monascus ruber NCPS2020003 is high in Monacolin K yield, no toxin substance citrinin is generated in the fermentation process, and the monascus ruber NCPS2020003 is stable in performance, high in growth speed and high in MK production capacity through fermentation. On the basis, burdock and purple sweet potato powder are used as main matrixes, the burdock functional red yeast rice is prepared through bag type fermentation, space is saved, the burdock functional red yeast rice is scattered once only when high-temperature fermentation is finished, then stirring and water supplementing are not needed in the whole low-temperature fermentation process, the fermentation time of the burdock functional red yeast rice prepared through the technology ranges from 15 days to 30 days, the Monacolin K content in the product reaches 0.5% or above, and the content of the monacolin K in the product reaches 0.5% or above. And the content of open-loop Monacolin K reaches 75% or above.

The invention relates to the technical field of biology, and particularly discloses construction and application of monascus overexpression GAD genetically engineered bacterium, and the construction of the monascus overexpression GAD genetically engineered bacterium comprises the following steps: constructing an expression vector pNeo-GAD recombinant plasmid, transforming the recombinant plasmid into agrobacterium, transforming monascus by an agrobacterium-mediated method, and carrying out PCR identification on monascus transformants. The application method comprises the following steps: culturing to obtain a seed solution of the monascus overexpression GAD genetically engineered bacterium, inoculating the seed solution into a fermentation culture medium, and carrying out fermentation culture to obtain a fermentation product of the monascus overexpression GAD genetically engineered bacterium; a fermentation product of the obtained monascus overexpression GAD genetic engineering bacterium contains high-yield monacolin K and high-yield gamma-aminobutyric acid, and meanwhile citrinin is not detected in the fermentation product of the monascus overexpression GAD genetic engineering bacterium.

This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for improving heart failure and its preparation method. The traditional Chinese medicine composition for improving heart failure comprises the following raw materials in parts by weight: astragaloside A 4-7 parts, arctiin 8-12 parts, citrinin 3-6 parts, and perilla lactone 2-4 parts. Experimental results show that taking the traditional Chinese medicine composition for improving heart failure prepared according to this invention has the effects of tonifying heart qi, warming yang and tonifying kidney, promoting blood circulation and detoxifying, and reducing swelling and promoting diuresis, thereby effectively improving cardiac function, inhibiting excessive activation of the RAAS system, reducing myocardial damage and myocardial fibrosis, and significantly alleviating heart failure symptoms. Furthermore, the traditional Chinese medicine composition provided by this invention has precise formulation, few toxic side effects, high safety, and broad application prospects.

The application provides a method for solid-state fermentation of poria cocos by purple monascus CSU-M183 and enzymes, and the method is characterized in that: the method is a biological transformation method, and the traditional Chinese medicine poria cocos is used as raw material, and the poria cocos is subjected to bidirectional solid-state fermentation by enzyme pretreatment and purple monascus CSU-M183 to improve the yield of Monacolin K, water-soluble polysaccharides and triterpenoid compounds, and no citrinin is generated. The method improves the efficient utilization of poria cocos resources, increases the added value of the poria cocos, provides a new processing technology guidance for poria cocos products, and provides a method for saving resources, reducing cost and high safety for high-value comprehensive utilization of the traditional Chinese medicine poria cocos.

The invention relates to the field of biological fermentation, and discloses red monascus for highly producing monacolin K and monascus pigment and application of the red monascus. The red monascus purpureus is named as SHM1108, the microorganism classification name of the red monascus purpureus is Monascus purpureus, the preservation number of the red monascus purpureus is CGMCC (China General Microbiological Culture Collection Center) NO.41565, the preservation date of the red monascus purpureus is September 24, 2024, and the preservation place of the red monascus purpureus is China General Microbiological Culture Collection Center. The red monascus not only can produce ring-opening monacolin K and monascus pigment at the same time, but also does not produce citrinin, and the monascus obtained through fermentation can be applied to the fields of food, health food, medicine and the like.

This invention proposes an analytical method for evaluating the quality of red yeast rice based on HPLC fingerprinting and multi-component quantitative analysis, belonging to the field of quality control technology for red yeast rice and related products. The method includes: taking a red yeast rice sample, ultrasonically extracting it with 50% methanol, centrifuging, and filtering to prepare a test solution; performing detection using high-performance liquid chromatography; constructing a red yeast rice fingerprint chromatogram based on the detection results, and simultaneously determining the content of six active ingredients: ankaflavin, citrinin, lactone-type mevastatin, lactone-type lovastatin, acid mevastatin, and acid lovastatin; and combining chemical pattern recognition methods to perform discriminant analysis on red yeast rice samples from different sources to evaluate the quality of the red yeast rice. This invention achieves an organic combination of simultaneous quantitative analysis of multiple components in red yeast rice and overall quality evaluation based on fingerprint chromatograms. Methodological validation shows that this method has good precision, repeatability, and stability, and is accurate and reliable, suitable for the quality control of red yeast rice raw materials and health foods and pharmaceuticals containing red yeast rice.

The invention provides monascus purpureus, a high-yield lovastatin and low-citrinin culture medium and a method, and belongs to the technical field of microbial fermentation. The method comprises the following steps: performing streak inoculation on Monascus purpureus HBSDYX-026 to a seed culture medium, and culturing to obtain a seed solution; and inoculating the seed solution to a high-yield lovastatin culture medium for fermentation. According to the invention, by optimizing the components of the culture medium, adjusting the variety and proportion of key nutrient substances, namely a carbon source and a nitrogen source, and screening and adding traditional Chinese medicines and an accelerant, a more suitable environment is provided for the growth of monascus and the synthesis of lovastatin; a monascus strain which is stable in heredity, vigorous in growth, high in lovastatin yield and low in citrinin yield is obtained, a culture medium which can efficiently synthesize lovastatin and is low in citrinin is successfully constructed, and the yield of lovastatin in red yeast rice is remarkably increased.

The invention discloses a method suitable for purification, enrichment and determination of citrinin in food, and belongs to the technical field of chemical analysis. The method suitable for purifying and enriching citrinin in food comprises the following steps: preparing a sample to be detected into an extracting solution to be purified; the extracting solution passes through a solid-phase extraction column with MAX serving as an upper-layer filler and GCB serving as a lower-layer filler, elution is conducted, and a citrinin-enriched solution to be detected is obtained. And further determining the content of citrinin in the to-be-detected liquid to obtain the content of citrinin in the food. The method provided by the invention is suitable for purification and enrichment of citrinin in various foods, can effectively control the influence of co-extraction impurities, and can obtain purification and recovery capacities comparable to those of an immunoaffinity column. The citrinin content of the enriched solution is further measured, and the citrinin content in various foods can be obtained. The detection method provided by the invention is simple and convenient to operate, low in cost, high in sensitivity and good in accuracy.

This invention relates to a method for preparing health-promoting red yeast rice wine, comprising: (1) preparing a liquid fermentation broth of *Pseudomonas ulmoides*; (2) preparing a starter culture, wherein the starter culture is obtained by inoculating *Rhizopus* into a starter culture made from glutinous rice, soybeans, banana peel, vitamin B2, vitamin C, and sterile water; (3) steaming and cooling glutinous rice and adding *Pseudomonas ulmoides* fermentation broth, *Monascus purpureus* expanded culture broth, decanoic acid, and *Lactobacillus plantarum* expanded culture broth, and saccharifying and fermenting to obtain a first fermented product; (4) adding starter culture to the first fermented product, stirring evenly, and continuing saccharification to obtain a second fermented product; (5) pumping the second fermented product into a fermentation tank for alcoholic fermentation; and (6) after the fermentation in step (4) is completed, clarifying, filtering, sterilizing, and bottling the finished product. By brewing rice wine using this method, vitamins and various amino acids from *Pseudomonas ulmoides* can be extracted into the rice wine, and various specific amino acids can reduce the yield of citrinin in red yeast rice under certain fermentation conditions, resulting in high-quality red yeast rice wine.

The invention provides application of tilianin in preparation of a medicine for preventing and treating femoral head necrosis, relates to the technical field of new application of medicines, and is technically characterized by providing application of tilianin in preparation of a medicine for treating steroid-induced femoral head necrosis. A rat steroid-induced femoral head necrosis model induced by LPS and large-dose glucocorticoid is adopted, and the treatment effect of tilianin on rat steroid-induced femoral head necrosis is observed. Carrying out three-dimensional analysis on the femoral head after Micro-CT scanning, and applying Hamp; the E staining is used for carrying out histopathologic analysis on femoral head tissues, and the treatment effect of tilianin on steroid-induced femoral head necrosis of rats is proved. In addition, SD rat BMSC is extracted, and the promoting effect of tilianin on osteogenic differentiation is further analyzed in vitro. Animal and cell experiments prove that tilianin can relieve the inhibition effect of glucocorticoid on femoral head bone tissues, and tilianin can effectively promote bone formation and improve osteogenesis activity of osteoblasts, so that the degree of femoral head necrosis is relieved, and a new way is provided for medicine prevention and treatment of steroid-induced femoral head necrosis.

The invention belongs to the field of biological detection kits, and particularly discloses a chemiluminescence immunoassay kit for detecting mycotoxin. The kit is based on a homogeneous immunoassay principle, and can quantitatively detect aflatoxin B1, vomitoxin, zearalenone, ochratoxin A and citrinin in a sample. The core of the invention is to provide an integrated detection system which comprises special reagent cards for the five toxins and a unified sample pretreatment module (an extracting solution and a diluent). Each reagent card is internally pre-coated with freeze-dried photosensitive microspheres and luminous microspheres coated with specific antibodies or antigens, and automatic identification and traceability of detection information are realized through two-dimensional codes. Through an optimized reagent formula and a preparation process, multi-index, high-throughput, rapid and accurate detection is realized, the problems of tedious operation, low efficiency, incapability of multi-joint detection and the like in the prior art are solved, and the method is suitable for rapid screening and monitoring of grain and oil food safety.

The invention relates to a modern solid-state fermentation high-activity polysaccharide monascus beautiful millettia root and a preparation method thereof, and belongs to the technical field of microbial solid-state fermentation. The product is prepared from Monascus purpureus Yang-Y3000 with the preservation number of GDMCC No: 64057 through solid state fermentation of the beautiful millettia roots, and the product is prepared from the beautiful millettia roots through the solid state fermentation of the Monascus purpureus Yang-Y3000 with the preservation number of GDMCC No: 64057. The preparation method comprises the following steps: cleaning and slicing beautiful millettia roots, pretreating the beautiful millettia roots with acidified water, mixing the pretreated beautiful millettia roots with rice flour and water according to a specific ratio, sterilizing, inoculating liquid seeds of the strains, fermenting at 30-35 DEG C for 100-144 hours, and finally drying at low temperature. According to the method, the high-efficiency biotransformation capability of a specific strain in an acidic solid environment is utilized, so that the content of crude polysaccharide and crude protein of the product is remarkably increased compared with that of raw materials, meanwhile, the content of total flavonoids is greatly reduced, the product does not contain harmful mycotoxins of monacolin K and citrinin, and the remodeling of the raw material component spectrum of the beautiful millettia root is successfully realized.

The invention is applicable to the technical field of microbial fermentation, and provides highland barley monascus and a preparation method thereof.According to the method, monascus magnetic microcapsules, bacillus coagulans pH trigger capsules and lactobacillus plantarum sustained-release microspheres are constructed respectively, and a plurality of microecological nichs which are clear in structure and physically separated are formed in a fermentation system; therefore, independent regulation and control on different microorganism growth microenvironments are realized; a space-time cascade fermentation process of'main fermentation space-enzymolysis space 'is adopted, so that the pre-transformation process of a substrate and the metabolic process of microorganisms are accurately butted and efficiently coordinated in time and space. The method effectively solves the problems of weak synergistic effect among different strains, low substrate conversion efficiency and high residual quantity of toxic metabolin citrinin in traditional solid state fermentation. According to the process, the content of the target active component Monacolin K can be increased by 110% or above, meanwhile, citrinin residues are strictly controlled to be 0.05 mg / kg or below, and the method has wide application prospects.

The invention relates to the field of food additive pigment extraction, in particular to a preparation method of monascus red pigment with low citrinin. According to the method for performing liquid submerged fermentation by adopting a monascus strain, vegetable oil is added into a culture medium, production of citrinin in the fermentation process is controlled in a mode of feeding hydrogen peroxide in the fermentation process, and monascus red pigment is extracted; the monascus purpureus is subjected to liquid culture, acid precipitation, filtration, washing and filtration, a filter cake is collected, and the filter cake is extracted and refined with food-grade ethanol. The content of citrinin in the monascus red pigment obtained by using the method is extremely low, the quality and safety of a monascus product are greatly improved, and the method has relatively high industrial application value and is beneficial to popularization and application of monascus red.

This invention discloses a Monascus purpureus strain that produces high levels of Monascus pigment and low levels of citrinin, and its applications. The Monascus purpureus strain producing high levels of Monascus pigment and low levels of citrinin is classified as *Monascus purpureus* FAAS-M34 (…). Monascus purpureus The purple Monascus strain FAAS-M34 has been deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2022959 and a deposit date of June 24, 2022. This strain exhibits a strong ability to produce natural red yeast rice pigment and low production of citrinin, making it suitable for preparing high-quality red yeast rice. The resulting red yeast rice product has a high color value, and the citrinin content does not exceed relevant national regulations, with a high product yield. Therefore, the purple Monascus strain FAAS-M34 can be safely used in the food processing industry. Compared with existing strains of the same type, it can produce higher-quality red yeast rice products and has high industrial application value.

The invention relates to a method for regulating and controlling production of citrinin from penicillium citrinum by exogenous signal molecules and application of the method in mildew prevention of food and agricultural products. Penicillium citrinum fermentation liquor is analyzed through metabonomics, and key signal molecules of farnesol, tyrosol, linoleic acid, cytidine, tryptophan and noradrenaline for regulating the growth of the penicillium citrinum are screened out. Based on the fact that the signal molecules can be used for regulating and controlling the growth retardation period, the maximum growth rate and the citrinin yield of penicillium citrinum, the action target of each signal molecule is clarified, meanwhile, verification is carried out in a Liupao tea system, the method utilizes a microbial quorum sensing mechanism, precise mildew prevention is achieved through targeted regulation and control of toxin-producing genes, and the application prospect is wide. The method has the characteristics of high efficiency and safety, provides a new environment-friendly method for food safety regulation and control of tea, feed and other related fermented products, and also provides a new view angle and method for research of microbial metabolism regulation and control.

The invention provides a monascus fermented corn strain screening method. The monascus fermented corn strain screening method comprises the following steps: step 1, strain activation; step two, preparing a spore suspension; 3, preparing a seed solution; and 4, fermenting, culturing and screening. The invention provides a monascus fermented corn strain screening method which comprises the following steps: collecting monascus in a main monascus producing area in China, carrying out monascus separation and purification, and identifying monascus by adopting three methods of monascus morphological identification, ITS molecular biological identification and 18S molecular biological identification; the separated strain is subjected to solid-state and liquid-state fermentation process exploration, and determination of fermentation product pigment, citrinin, lovastatin and the like is carried out, so that high-quality monascus can be screened out, and a strain resource is provided for production of corn monascus.

The application discloses a method for synchronous detection of multiple mycotoxins. After crushing the sample to be detected, the sample is pretreated by using a hierarchical QuEChERS extraction method and / or a solid-phase extraction method according to the characteristics of the matrix to prepare a sample extract. A mixed control standard working solution containing aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, citrinin, ochratoxin A and zearalenone is prepared, and the seven mycotoxins are detected by using ultra-high performance liquid chromatography-tandem mass spectrometry. Qualitative and quantitative analysis is realized by alternately scanning positive and negative ions and multi-reaction monitoring. The method can be adapted to the need for mycotoxin control of traditional Chinese medicine decoction pieces, does not need an immunoaffinity column, has high pretreatment efficiency, low detection cost and high throughput, can realize synchronous and accurate detection of multiple toxins, and meets the demand for rapid screening of large batches of traditional Chinese medicine decoction pieces.

The present application relates to the field of microbial fermentation, and discloses a solid-state fermentation method for high-yield production of Monascus yellow pigment by Monascus purpureus. By regulating key process parameters such as fermentation temperature, gelatinization amount, fermentation time, inoculation amount, loading amount of solid medium for fermentation, and types of carbon source matrix, the present application realizes efficient directional biosynthesis and enrichment of Monascin (MS) and Ankaflavin (AK). The method discards the traditional vague color value index, establishes a quality standard with MS and AK specific content as the core, and promotes the product from a food coloring agent to a medicinal raw material. The target components are enriched by solid-state fermentation technology, and the extraction and purification steps are omitted, thereby greatly reducing the cost. The finished product is Monascus powder, which is consistent with the traditional application scene. The final product has the high MS / AK content, low MK content, and no citrinin, and meets the high-end medicinal raw material standard.

This invention discloses a Monascus strain that produces high levels of Monascus pigment and its applications. The Monascus strain producing high levels of Monascus pigment is classified as Monascus purpureus FAAS-M3 (…). Monk purple The purple Monascus FAAS-M3 strain provided in this invention has been deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2022958 and deposit date of June 24, 2022. This strain exhibits a strong ability to produce natural Monascus pigments and also produces high levels of citrinin. Red yeast rice products produced using this strain have high color value and citrinin content, resulting in a high product yield. Therefore, the purple Monascus FAAS-M3 strain provided in this invention has high research value.

The invention relates to the technical field of food safety, in particular to a method for removing citrinin, which comprises the following steps: puffing reduced protein by using a microwave puffing method, and inducing the reduced protein to form a stable three-dimensional porous structure; the method comprises the following steps: dissolving protein in pure water to prepare protein liquid with a certain concentration, transferring the protein liquid into a pressure-resistant reaction kettle, adding a proper amount of a catalyst, introducing hydrogen, and reacting; the prepared protein porous material is used for effectively adsorbing citrinin molecules. The method is simple in process and convenient to operate, the content of citrinin in food can be remarkably reduced, food safety is guaranteed, and human health is protected.

Provided are a monascus strain capable of producing monascin and / or ankaflavin and the use thereof. Particularly, provided are a new monascus strain assigned with the accession number CGMCC NO. 41335 and the use thereof in the production of high-content monascin and / or ankaflavin and related products thereof. The strain is Monascus purpureus ZYXQ-2206, can produce potent and non-toxic lipid-lowering ingredients monascin and / or ankaflavin with a high yield, and does not produce harmful and toxic ingredients lovastatin (LOV) and citrinin (CIT). Since excessive intake of LOV can cause serious side effects such as liver and kidney injury and rhabdomyolysis, and excessive intake of CIT can cause toxic responses such as renal tubular dilation and degeneration and necrosis of epithelial cells, the monascus products produced by using the strain can effectively avoid these safety risks, and have extremely high application prospects and market potentials. By screening and optimizing a fermentation process, the monascus strain having high productivity, no toxicity and high functionality is provided, which has significant application value in the fields of development of lipid-lowering functional foods and dietary supplements.

The invention belongs to the field of biological detection kits, and discloses a method for detecting mycotoxin through laser-excited chemiluminescence. The core of the method is that a laser beam with the wavelength of 680 + / -10nm and the power density of 100-500mW / cm < 2 > is adopted to excite the photosensitive microspheres in a detection system in a pulse or continuous scanning mode, and chemiluminescence signals generated after the detection are detected, so that the detection sensitivity of the photosensitive microspheres is improved. The rapid, parallel and quantitative detection of five mycotoxins, namely aflatoxin B1, vomitoxin, zearalenone, ochratoxin A and citrinin, in a single sample is realized. The system includes a specific laser source, a sample compartment, a photodetector, and a processor. The matched kit provides a unified sample pretreatment solution and independent freeze-drying reagent cards of various toxins. The excitation efficiency and the signal-to-noise ratio are improved by optimizing laser parameters, the problems that in the prior art, sensitivity is insufficient, operation is tedious and flux is low are solved in combination with the mode of one liquid and multi-detection parallel, and the method is suitable for large-scale rapid screening of grains and products of the grains.

The application provides a microbial source antioxidant pigment feed additive for aquaculture and a preparation method thereof, and comprises the following steps: generating a mutant strain Talaromyces albobiverticillius CY110 by subjecting Talaromyces albobiverticillius CY-G to low-temperature plasma physical mutagenesis, and then producing wine red pigment by utilizing the mutant strain Talaromyces albobiverticillius CY110 to carry out semi-solid state fermentation, the pigment product obtained by the application has high yield of red pigment, orange pigment and yellow pigment, low content of citrinin, stable properties, and strong antioxidant capacity, and can be used for microbial source antioxidant pigment feed additives for aquaculture, and has a wide application prospect. The preparation method of the application is simple in operation, low in equipment requirement, and high in production efficiency, and is suitable for large-scale production.