Dimer citrinin compounds and preparation and use thereof
A kind of compound and application technology, applied in the field of preparing cell proliferation inhibitor
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Embodiment 1
[0013] Fermentative production and separation and purification of compound I and II of embodiment 1
[0014] 1 Fermentation production
[0015] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take an appropriate amount of Penicillium citrinum HGY1-5 (preservation number: CCTCC M 208170), inoculate it on the PDA solid slant medium, and cultivate it at 28 degrees Celsius 4 days in the box.
[0016] Take an appropriate amount of Penicillium citrinum HGY1-5 (Penicillium citrinum) cultured on the slant for 4 days, and inoculate it into 200mL culture medium [medium composition (g / L): maltose 20.0, mannitol 20.0, monosodium glutamate 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, yeast extract 3.0, pH natural] in a 500mL Erlenmeyer flask, cultured on a shaker at 28°C and 160 rpm for 15 days to obtain mycelium and fermentation broth.
[0017] 2 Obtaining the extract
[0018] Separate the mycelium and fermentation broth with a c...
Embodiment 2
[0033] The test of embodiment 2 in vitro antitumor activity
[0034] Cell Proliferation Inhibition Activity Test and Cell Apoptosis Induction Activity Test
[0035] 1 Experimental samples and experimental methods
[0036] Preparation of test sample solution The test samples are pure compounds I and II isolated and refined in Example 1 above. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.
[0037] Cell lines and subculture of cells use cancer cell lines such as human lung cancer A549 cells, human liver cancer BEL-7402 cells, human leukemia HL60 cells and human leukemia MOLT-4 cells. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.
[0038] Cell Proliferation Inhibitory Activity Test Method
[0039]Human lung cancer A549 cells and human liver cancer BEL-7402 cells in the logarithmic growth phase wer...
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