699results about How to "Good biological properties" patented technology

The present invention provides novel peptidomimetic macrocycles and methods for their preparation and use, as well as amino acid analogs and macrocycle-forming linkers, and kits useful in their production.

Anti-CD19 B4 antibodies with modified variable regions are disclosed. The modified anti-CD19 variable region polypeptides have alterations to one or more framework regions or complementarity determining regions of the heavy chain variable region or light chain variable region, thereby to reduce a T-cell response.

The invention is directed to a fusion protein which includes a first portion including an immunoglobulin (Ig) chain and a second portion including interleukin-7 (IL-7).

The invention provides a chimeric T cell receptor (TCR) comprising a variable region of a human TCR and a constant region comprising at least an extracellular domain of a constant region of a non-human TCR, as well as functional variants thereof. The invention also provides polypeptides and proteins related to the inventive TCRs, as well as nucleic acids encoding the TCRs, polypeptides, or proteins, recombinant expression vectors, and host cells. Further provided are pharmaceutical compositions related to the inventive TCRs and methods of preventing or treating a disease, e.g., an infectious disease, cancer, in a host, methods of detecting a diseased cell in a host, and methods of improving the biological activity of a TCR.

Modified interleukin-7 (IL-7) polypeptides are disclosed. The modified IL-7 polypeptides have alterations to one or more potential T-cell epitopes, thereby to reduce a T-cell response.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium / micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention provides human extracellular signaling molecules (EXCS) and polynucleotides which identify and encode EXCS. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with expression of EXCS.

The invention relates to a pig feed additive capable of replacing antibiotics, and a preparation method and an application thereof. Components and weight part ratio thereof in a formula are as follows: 1-5 parts of medicated leaven, 1-5 parts of angelica sinensis, 1-5 parts of radix astragali, 1-5 parts of hawthorn, 1-5 parts of radix codonopsis, 10-20 parts of citric acid, 10-20 parts of lactic acid, 20-30 parts of coated phosphoric acid, 5-10 parts of a micro-ecological preparation, 5-10 parts of yeast cell wall polysaccharide, 1-5 parts of a composite plant essential oil and 5-10 parts of a carrier. The pig feed additive carries out combination collocation of traditional Chinese herbs and functional polysaccharide, the micro-ecological preparation, acidifying agents, plant essential oil and the like, so that synergic effect of the traditional Chinese herbs and other components are maximized; the pig feed additive can replace the antibiotics completely; problems of drug resistance, drug residues, overcommitment and the like of the antibiotics which trouble animal husbandry for a long time is reduced; and at the same time, the pig feed additive plays effects of improving culture environment, reducing feed safety hidden dangers, increasing culture benefits and the like.

Disclosed are Fc-interferon-beta (Fc-IFN-β) fusion proteins and nucleic acid molecules encoding them. The Fc-IFN-β fusion proteins include variants of the interferon-beta (IFN-β) protein that are altered to achieve enhanced biological activity, prolonged circulating half-life and greater solubility. Also disclosed are methods of producing the fusion proteins and methods of using the fusion proteins and / or nucleic acid molecules for treating diseases and conditions alleviated by the administration of interferon-beta.

Disclosed are nucleic acid sequences, for example, DNA or RNA sequences, which encode an immunoglobulin Fc-Interferon-alpha fusion protein. The nucleic acid sequences can be inserted into a suitable expression vector and expressed in mammalian cells. Also disclosed is a family of immunoglobulin Fc-Interferon-alpha fusion proteins that can be produced by expression of such nucleic acid sequences. Also disclosed are methods of using such nucleic acid sequences and / or fusion proteins for treating conditions, for example, hepatitis, which are alleviated by the administration of interferon-alpha.

Peptidomimetic macrocycles and methods for their preparation and use, as well as amino acid analogs and macrocycle-forming linkers, and kits useful in their production, are provided.

The present invention discloses the method of compounding aquogel into porous tissue engineering rack. Under sol state, aquogel is first led into porous rack, and the outer condition is altered to convert the sol into gel to obtain porous rack with compounded aquosol. According to requirement, the water sol may have cell growth factor for cell growth and differentiation may be added. The rack the present invention produces possesses the excellent mechanical performance of porous rack and the excellent biocompatibility of aquosol rack simultaneously. The present invention has wide application range, and is suitable for various porous rack with different structures and aquosols and various kinds of cell. The rack may be used widely in tissue engineering field.

The invention relates to a bracket material used for an osseous tissue project, and a preparation method thereof. Firstly, the bracket of a type I collagen is extracted from a small fresh pigskin by a mature extracting technique. Then, the bracket is further expanded in a Tris cushion liquid, the pH of which is equal to 8.8 to obtain a natural porous collagen bracket by the treatments of freezing and drying. The bracket is respectively and repeatedly mineralized in a CaCl2 liquid and in (NH4)2HPO4 liquid or mineralized in simulated body fluid for a long period to lead the weakly crystallized HA to be uniformly settled into the collagen bracket; and then a pigskin collagen-hydroxyapatite ossein is obtained by the treatments of freezing and drying to replace the natural bracket material. The invention not only maintains the natural bracket structure of the collagen in an organism, but also has the advantages of low material cost, simple devices, short period and easy operation. The obtained compound bracket material used for the pigskin collagen-hydroxyapatite osseous tissue project has the characteristics of high intensity, large toughness, non-antigenicity, higher biological activity as well as degradation and releasing control.

The invention discloses a small-caliber silk fibroin tubular material and a preparation method thereof and belongs to the field of biomedical materials. The tubular material consists of three layers of materials which are firmly jointed, wherein an outer layer is a tubular electrostatic spinning silk net; a middle layer is a netlike pipe formed by weaving bright silk prepared from degummed silk; and an inner layer is a silk porous material. After the processes of soaking, drying, curing and the like in silk solution, the three layers of the material are firmly jointed, and the inner layer is treated by using anti-coagulation medicaments such as heparin and the like so that the material has coagulation resistance; and the tubular material obtained by using a forming die consisting of a cylindrical intermediate (1), a hollow cylinder (2) and a round base (3) has the inner diameter of between 1 and 8 millimeters, the thickness of between 0.2 and 2 millimeters, high mechanical property, biocompatibility, coagulation resistance and capability of repairing tissues such as blood vessels, nerves and the like.

The invention relates to a natural active drug-polysaccharide targeted compound with antitumor activity and targeting property. A natural active drug is introduced onto a polysaccharide framework by chemical grafting, and physically mixed with a ligand distearoylphosphatidyl ethanolamine-polyethyleneglycol-anisoyl amine with targeted transmission capacity to form a natural active drug-polysaccharide targeted compound, and the natural active drug-polysaccharide targeted compound can be self-assembled in water to form a nano micelle. The method is characterized in that the natural active drug-polysaccharide targeted compound has targeted transmission capacity, enhances the tumor transfer efficiency of the preparation, reduces the untoward reaction and enhances the tolerance of the patient; the hydrophobic inner core formed by the hydrophobic group can physically wrap the antineoplastic drug, thereby obviously improving the water solubility of the antineoplastic drug; and the antineoplastic drug which is physically wrapped by the chemically coupled ursolic acid can implement combined treatment of tumors and lower the toxic or side effect. The preparation method is simple and easy to operate, has the advantage of higher yield, and can easily implement industrialization.

The invention provides a serum-free cell culture fluid suitable for enriching and culturing tumour stem cells. DMEM / f12 serves as the basic culture fluid of the serum-free cell culture fluid and transferrin, insulin and other substances are also added as the components of the serum-free cell culture fluid. The serum-free cell culture fluid provided by the invention can efficiently enrich tumour stem cells from malignant tumour cell strains and tumour tissues. In addition, the serum-free cell culture fluid can promote stable growth of the tumour stem cells and maintain fine cell activity and physiological properties of the tumour stem cells, thus being very suitable for research fields related to tumour cells and tumour stem cells.

Disclosed are a macroscopic-microcosmic-nanometer hierarchical mechanical compatible bone restoration and preparation thereof. The bone restoration comprises a macroscopic pore metal structural body, a microscopic pore structural body and nanofibers. The appearance of the macroscopic pore metal structural body is consistent with an anatomic form of defect part bone tissues, the size of internal macroscopic pores is 300-1500 micrometers, porosity ranges from 50% to 90%, microscopic roughness Ra of pore walls is 5-30 micrometers, all the macroscopic pores are communicated with one another completely, mechanical property of the macroscopic pore metal structural body is compatible with that of the defect part bone tissues, compressive strength is 60-300MPa, and elasticity modulus is 0.5-30GPa. The microscopic pore structural body is positioned in the macroscopic pore metal structural body, internal microscopic pores are uniform in structure and are communicated with one another completely, the pore size is 50-250 micrometers, and pore walls of the microscopic pores consist of the nanofibers which are made of biodegradable polymer materials. The bone restoration perfectly integrates the anatomic form, the mechanical property and biological property and can be applied to treatment of large segmental bone defects of bearing parts clinically.

The instant invention relates to modulated lysine variant species compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and / or protein purification methods, for producing such modulated lysine variant species compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

The invention provides human receptors and membrane-associated proteins (REMAP) and polynucleotides which identify and encode REMAP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of REMAP.

The invention discloses a multienzyme organic fertilizer, wherein the fertilizer can increase soil organic matter contents and varieties of bioactivators, and improve physical, chemical and biological characteristics for soil, and improve soil fertility, and alleviate soil hardened state, and reduce pesticide residue in the soil; on the other hand, the fertilizer can provide progressive, continuous and complete nutrients for plant growth, and improve inner environment of crops at the same time such that the crops have abilities of full digestion and nutrient absorption. The invention can not only improve the yields of the crops, but also can improve qualities of agricultural products, and enhance healthcare functions for agricultural products. The fertilizer is suitable to be used by field crops and greenhouse vegetables, land vegetables, fruits, flowers, commercial crops and so on. The multienzyme organic fertilizer is composed of the following raw materials in percentages by weight: 10-15% of laccase, 5-10% of neutral protease, 10-15% of phytase, 5-10% of cellulose, 1-5% of xylanase, 1-5% of pectinase, 1-5% of amylase and 55-60% of organic fertilizer powder.

The invention provides high-resilience antibacterial medical polyvinyl chloride (PVC) plastic and a preparation method thereof. The high-resilience antibacterial medical PVC plastic comprises, by weight, 100 parts of ultra-high molecular weight PVC resin, 40-80 parts of a plasticizer, 10-60 parts of an elastomer, 5-20 parts of an auxiliary plasticizer and 0.5-5 parts of a heat stabilizer. The invention relates to the high-resilience antibacterial medical PVC plastic which has good physical properties, chemical performances and biological properties, satisfies GB15593-1995 blood (liquid) transfusion device flexible PVC plastic related requirements and is medical PVC plastic with excellent resilience and antibacterial and bacteriostatic effects, and the preparation method thereof. The PVC plastic has the characteristics of excellent antibacterial property, bacteriostasis and resilience, shortens an ethylene oxide disinfection period and improves disinfection efficiency.

The present invention relates to the identification of new proteins comprising fibroblast growth factor 21 (FGF21) and other metabolic regulators, including variants thereof, known to improve metabolic profiles in subjects to whom they are administered. Also disclosed are methods for treating FGF21-associated disorders, GLP-1-associated disorders, and Exendin-4-associated disorders, including metabolic conditions.

A thermo-responsive hydrogel, including a biocompatible monomer and / or polymer having an amino acid side chain. The hydrogel is thermo-responsive at a physiological temperature, and can include, incorporate, or encapsulate a treatment agent, such as a drug composition, a biomolecule, and / or a nanoparticle. The hydrogel is useful in delivering the treatment agent. The hydrogel is in a first physicochemical state for administration to a mammal. The hydrogel is thermo-responsive at a physiological temperature of the mammal, and changes to a second physicochemical state that is more solid than the first physicochemical state. In the second physicochemical state the thermo-responsive hydrogel releases the treatment agent.

The invention provides a collagen matrix freezing gel used for biomedical materials and a preparation method thereof. The collagen matrix freezing gel is the collagen extracted from skins or tendons of healthy domestic animals using the enzyme method with the molecular weight of 280 to 320kDa and a well maintained triple helical structure; the collagen reacts for 1 to 7 days in the condition of low temperature of 0 to minus 50 DEG C in a die after through cross linked and modified reaction with hydroformylation polysaccharide, then extrudes the die and is defrosted, thus forming the collagen matrix freezing gel. The preparation of the collagen matrix freezing gel of the invention in particular relates to the extraction of the collagen, the preparation of the hydroformylation polysaccharide and the synthesis of hydroformylation polysaccharide-collagen matrix freezing gel. The hydroformylation polysaccharide-collagen matrix freezing gel prepared by the invention improves the mechanical property, thermal stability and anti-enzyme degradation, etc., of pure collagen gels, and the freezing gel has the advantages of porosity, plasticity, hydrophilic property and non-toxicity, and can be used as biomedical materials such as bio-scaffold, cell cultivation, drug controlled release and biological dressings.