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Bracket material for bone tissue engineer and preparation method thereof

A technology of bone tissue engineering and scaffold materials, applied in medical science, prostheses, etc., can solve the problems of expensive scaffolds, destruction of the natural structure between collagen bundles, high antigenicity, etc., and achieve good mechanical properties and biocompatibility, The preparation process is green and environmentally friendly, and the cost is low

Inactive Publication Date: 2009-04-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scaffold obtained by this method is not only expensive, but also destroys the natural structure between collagen bundles, making it difficult to meet the mechanical and biological performance requirements of bone substitute materials
At the same time, in the process of dissolving collagen by various means, its "terminal peptide" is likely to be destroyed, thus causing collagen to have high antigenicity to tissues and affecting the growth of osteoblasts

Method used

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  • Bracket material for bone tissue engineer and preparation method thereof
  • Bracket material for bone tissue engineer and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The extracted pigskin collagen with a purity of up to 96% was cut into a block frame of length: width: height = 1 cm × 1 cm × 0.2 cm with a stainless steel blade. Soak the stent in Tris buffer solution with pH=8.8 (the preparation method is to dissolve 121.1g Tris base in 800ml water at 25°C, add concentrated HCL to adjust the pH value to 8.8, and continue to add water to 1 liter), and place it at 37°C Make it fully swell in a shaker for 24 hours, take it out and rinse it with deionized water for 3 times, 20 minutes each time. Take out the bulk collagen, wipe off the surface moisture with filter paper, put it into a liquid nitrogen bucket to freeze for 2 hours, freeze-dry at -80°C for 8 hours, and obtain fully expanded pure collagen scaffold A. Prepare 0.25M CaCl separately 2 Solution B and 0.25M (NH 4 ) 2 HPO 4Solution C 1L each. Soak A in 25ml of B solution, and shake in a shaker at 37°C at a speed of 120 rpm for 2 hours, take it out and wash it with deionized wa...

Embodiment 2

[0030] The extracted pigskin collagen with a purity of up to 96% was cut into a block frame of length: width: height = 1 cm × 1 cm × 0.2 cm with a stainless steel blade. Soak the scaffold in Tris buffer solution with pH=8.8, put it in a shaker at 37°C to fully swell for 24 hours, take it out and wash it with deionized water for 3 times, 20 minutes each time. Take out the bulk collagen, wipe off the surface moisture with filter paper, put it into a liquid nitrogen bucket for 2 hours, and then freeze it at -80°C for 8 hours to obtain a fully expanded pure collagen scaffold. At 37°C, prepare a standard simulated body fluid (in which: Na + 142.0,K + 5.0, Mg 2+ 1.5, Ca 2+ 2.5, Cl - 148.8, HCO 3 - 4.2, HPO 4 3- 1.0, SO 4 2- 0.5(mM)) 1L, adjust the pH=7.4 with Tris buffer solution of pH=8.8 and 0.1M HCl, immerse the collagen scaffold in the simulated body fluid, and shake at a speed of 120 rpm in a shaker at 37°C After 6 days (the mineralization solution was changed every ...

Embodiment 3

[0033] The extracted pigskin collagen with a purity of up to 96% was cut into a block frame of length: width: height = 1 cm × 1 cm × 0.2 cm with a stainless steel blade. Soak the scaffold in Tris buffer solution with pH=8.8, put it in a shaker at 37°C to fully swell for 24 hours, take it out and wash it with deionized water for 3 times, 20 minutes each time. Take out the bulk collagen, wipe off the surface moisture with filter paper, put it into a liquid nitrogen bucket and freeze it for 2 hours, and freeze-dry it at -80°C for 80 hours to obtain fully expanded pure collagen scaffold A. Prepare 9mM CaCl separately 2 Solution B and 4.2mM (NH 4 ) 2 HPO 4 For solution C, mix B and C at a volume ratio of 1:1. At 37°C, adjust the pH to 7.4 with Tris buffer at pH 8.8 and 0.1M HCL. Soak A in the mixed solution of B and C, and shake it in a shaker at 37°C at a speed of 120 rpm for 6 days (change the mineralization solution every two days), take it out and wash it with deionized wa...

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Abstract

The invention relates to a bracket material used for an osseous tissue project, and a preparation method thereof. Firstly, the bracket of a type I collagen is extracted from a small fresh pigskin by a mature extracting technique. Then, the bracket is further expanded in a Tris cushion liquid, the pH of which is equal to 8.8 to obtain a natural porous collagen bracket by the treatments of freezing and drying. The bracket is respectively and repeatedly mineralized in a CaCl2 liquid and in (NH4)2HPO4 liquid or mineralized in simulated body fluid for a long period to lead the weakly crystallized HA to be uniformly settled into the collagen bracket; and then a pigskin collagen-hydroxyapatite ossein is obtained by the treatments of freezing and drying to replace the natural bracket material. The invention not only maintains the natural bracket structure of the collagen in an organism, but also has the advantages of low material cost, simple devices, short period and easy operation. The obtained compound bracket material used for the pigskin collagen-hydroxyapatite osseous tissue project has the characteristics of high intensity, large toughness, non-antigenicity, higher biological activity as well as degradation and releasing control.

Description

technical field [0001] The invention relates to a tissue engineering scaffold material and a preparation method thereof, in particular to a pigskin collagen-hydroxyapatite natural scaffold material for bone tissue engineering and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] The defects of bone or cartilage tissue caused by traffic accidents, construction accidents and other trauma reasons often occur, which brings a lot of inconvenience to the daily life of patients and their families. Millions of people currently suffer from bone defects. It is reported that there are more than 450,000 bone graft operations and about 250,000 knee joint operations per year in the United States alone (C.T. Laurencin et al., Clin. Orthop. Relat. Res. 447 (2006) 221-236. E.B. Hunziker et al., Osteoarthr . Cartil. 10 (2002) 432-463.). At present, bone grafting is often used for repair in clinical work. However, these materials for b...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/12
Inventor 刘宏赵洪石王冠聪任娜刘铎王继扬陶绪堂蒋民华
Owner SHANDONG UNIV
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