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Collagen base freezing gel suitable for biological medical material and preparation thereof

A biomedical material, cryogel technology, applied in the direction of medical science, non-active ingredients of polymer compounds, absorbent pads, etc., can solve the problem that the mechanical properties of collagen gel, thermal stability and resistance to enzymatic degradation cannot meet the material performance requirements, The modified operation method is not easy to control, and produces inflammatory reactions, etc., to achieve the effect of good biodegradability, excellent biological characteristics, and low immunogenicity

Active Publication Date: 2008-08-06
北京益而康生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a collagen-based cryogel suitable for biomedical materials and its preparation method, to solve the existing problems in the preparation of collagen gel in the prior art, the modification operation method is not easy to control, and the prepared collagen gel Glue is easily hydrolyzed in the body to produce metabolically toxic free small molecule aldehydes, which can inhibit cell growth, produce inflammatory reactions, and even calcify in the body, or the mechanical properties, thermal stability, and enzymatic degradation resistance of collagen gels cannot meet the requirements of materials. The question of performance requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Fresh pigskin is cut into small pieces after removing hair, grease and other impurities on the skin surface, degreased with acetone for about 24 hours, dried and crushed into crumbs. Weigh 10 parts of skin scraps, put them into a reaction kettle with a stirring device and a thermometer, add 0.5mol / L acetic acid solution 80 times the weight of skin scraps, and then add pepsin with 2% of the weight of skin scraps, and heat at about 20°C After stirring for about 72 hours, the enzymolysis solution was obtained by centrifugation. The enzymatic hydrolysis solution is subjected to (NH4)2SO4 salting-out treatment at about 5°C, followed by dialysis and freeze-drying to prepare a dry collagen product.

[0028] Weigh 10 parts of raw starch, add it to a reactor equipped with a stirring device and a thermometer, then add 20 times the volume of distilled water, 10 parts of sodium periodate, adjust the pH value to 3, and stir the reaction in a water bath at about 35°C After about 4 h...

Embodiment 2

[0031] Fresh beef Achilles tendon is cut into small pieces after removing surface oil and impurities, degreased with acetone for about 12 hours, dried and crushed into scraps. Take by weighing 10 parts by weight of Achilles tendon scraps, add in the reaction kettle with stirring device and thermometer, add 0.5mol / L acetic acid solution of 80 times of scraps weight, then add pepsin of scraps weight 2%, in After stirring the enzymolysis at about 20°C for about 48 hours, centrifuge to obtain the enzymolysis solution. The enzymatic hydrolysis solution is subjected to NaCl salting-out treatment at about 5° C., and then dialysis and freeze-drying to prepare a dry collagen product.

[0032] Weigh 10 parts of chondroitin sulfate, add it to a reactor equipped with a stirring device and a thermometer, then add 20 times the volume of distilled water, 15 parts of sodium periodate, adjust the pH value to 1.5, and stir in a water bath at about 35°C React for about 3 hours. After the reacti...

Embodiment 3

[0035] Fresh beef Achilles tendon with surface grease and impurities removed, cut into small pieces, soaked in acetone for about 12 hours to degrease, dried and crushed into scraps. Weigh 10 parts of crumbs, add them to a reaction kettle with a stirring device and a thermometer, add 0.5mol / L acetic acid solution that is 80 times the weight of crumbs, and then add pepsin at 2% of the weight of crumbs. After stirring the enzymolysis for about 48 hours, centrifuge to get the enzymolysis solution. The enzymolysis solution is subjected to NaCl salting-out treatment at about 5°C, and then dialysis and freeze-drying to prepare dry collagen products.

[0036] Weigh 10 parts of hyaluronic acid, add it to a reactor equipped with a stirring device and a thermometer, then add 20 times the volume of distilled water and 20 parts of sodium periodate, adjust the pH value to 1.2, and stir in a water bath at about 35°C React for about 2.5 hours. After the reaction, add acetone 5 times the volu...

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Abstract

The invention provides a collagen matrix freezing gel used for biomedical materials and a preparation method thereof. The collagen matrix freezing gel is the collagen extracted from skins or tendons of healthy domestic animals using the enzyme method with the molecular weight of 280 to 320kDa and a well maintained triple helical structure; the collagen reacts for 1 to 7 days in the condition of low temperature of 0 to minus 50 DEG C in a die after through cross linked and modified reaction with hydroformylation polysaccharide, then extrudes the die and is defrosted, thus forming the collagen matrix freezing gel. The preparation of the collagen matrix freezing gel of the invention in particular relates to the extraction of the collagen, the preparation of the hydroformylation polysaccharide and the synthesis of hydroformylation polysaccharide-collagen matrix freezing gel. The hydroformylation polysaccharide-collagen matrix freezing gel prepared by the invention improves the mechanical property, thermal stability and anti-enzyme degradation, etc., of pure collagen gels, and the freezing gel has the advantages of porosity, plasticity, hydrophilic property and non-toxicity, and can be used as biomedical materials such as bio-scaffold, cell cultivation, drug controlled release and biological dressings.

Description

technical field [0001] The present invention relates to the technical field of collagen cryogel (cryogel), more specifically, relates to the technical field of preparing cryogel from collagen extracted from livestock animal skin or tendon, which is suitable for biomedical materials. Background technique [0002] Hydrogels are widely used in biomedical fields such as biological scaffolds, cell culture, drug controlled release, and biological dressings. Among them, hydrogels used in tissue engineering are mainly divided into two categories: synthetic polymer hydrogels and natural polymer water gels. gel. Collagen, as an important natural polymer gel raw material, can be used to form gels directly or as a matrix for composite gels. It has been widely used in the field of biomedical materials, such as using collagen-based gels as biological scaffolds to cultivate fibers Primary cells, epithelial cells, etc., or as wound dressings, etc. [0003] It is known that collagen is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L15/32A61K47/42
Inventor 林炜穆畅道刘芳张广照
Owner 北京益而康生物工程有限公司
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