32 results about "Biological dressing" patented technology

The invention relates to the field of biological dressings, and discloses an artificial dermis dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: adding water into a container, and freezing until the water is frozen; then adding a collagen A solution, freezing and standing; continuing to add collagen B liquid, freezing and standing; continuing to add collagen A liquid, freezing and then freeze-drying; and cross-linking in a glutaraldehyde solution, and cleaning to obtain the artificial dermis dressing. The dressing provided by the invention can provide a good stent effect and promote cell migration and proliferation, and the healing rate is close to 100% after wound making. In the wound healing process, the dressing can inhibit collagen deposition, relieve inflammatory response, inhibit abnormal hyperplasia of blood vessels, inhibit excessive proliferation of fibroblasts and excessive formation of granulation tissues, and effectively prevent scar formation. The dressing can also effectively inhibit abnormal vascular hyperplasia in a wound area, and fibrosis and scar hyperplasia caused by excessive vascular hyperplasia are prevented.

The invention relates to the technical field of biology, and particularly discloses polypeptide, recombinant XIX type humanized collagen and application of the recombinant XIX type humanized collagen. The amino acid sequence of the polypeptide comprises n repetitive units; the repetitive unit comprises any one of the following amino acid sequences or variant sequences thereof: (1) an amino acid sequence as shown in any one of SEQ ID NO.1-2; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence as shown in any one of SEQ ID NO.1-2; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in any one of SEQ ID NO.1-2; wherein n is an integer greater than or equal to 1. The polypeptide provided by the invention has a collagen triple helix structure, has good cell adhesion activity, can carry out functions of human collagen, is free of immunological rejection and anaphylactic reaction, and can be used in various fields of cartilage repair, biological dressing, tissue filling and the like.

The invention relates to the technical field of biological materials, in particular to an easy-to-produce recombinant humanized collagen purification process which comprises the following steps: performing centrifugal separation on a fermentation broth of a pichia pastoris expression strain to obtain a fermentation supernatant, and performing ultrafiltration concentration to obtain a fermentation supernatant concentrated solution; adding an ammonium sulfate solution into the fermentation supernatant concentrated solution, stirring, carrying out centrifugal separation, and collecting a precipitate I; dissolving the precipitate I with purified water, stirring and dissolving, repeating the step S2 again, and collecting a precipitate II; and dissolving the precipitate II with purified water, and then carrying out ultrafiltration, liquid change and concentration to obtain a purified recombinant humanized collagen solution. The recombinant humanized collagen purification process provided by the invention is simple in pretreatment process, easy to produce, relatively low in cost, small in protein loss and relatively high in protein recovery rate and purity; the prepared recombinant humanized collagen can be used as a raw material in the industries of medical beauty masks or biological dressings and the like, and can also be used for a subsequent refined chromatography process.

Provided is a biosynthesis-based preparation method for a structural material of the human body. A polypeptide of the present application contains the structure of a C-terminal region of (repeating unit)n or (repeating unit)n-; the repeating unit contains an amino acid sequence as shown in SEQ ID NO. 1. Recombinant type V humanized collagen prepared in the present application has high activity in promoting cell adhesion, and does not cause an immune response when applied to the human body; moreover, the preparation method is novel, allows for large-scale production of the recombinant type V humanized collagen, and is widely used in the preparation of structural materials of the human body. The application field includes preparation of high-end medical devices, such as biological dressings, human body biomimetic materials, plastic-beauty materials, organoid culture, cardiovascular stents, coatings, tissue injection filling, ophthalmic materials, gynecological biomaterials, nerve repair and regeneration, liver tissue and vessel repair and regeneration, and 3D printing of artificial organ biomaterials.

The invention belongs to the technical field of medical biological dressings, and provides a composite dressing with oxygen release and insulin delivery functions and a preparation method thereof.The composite dressing comprises a supporting base layer, a function slow release layer and a protective film layer which are sequentially compounded from bottom to top; the supporting base layer is a medical porous polyurethane film modified by plasma; the functional sustained release layer is composed of hyaluronic acid-chitosan composite gel, oxygen release microspheres and insulin liposome; the protective film layer is a polyethylene terephthalate film of which the surface is provided with a sawtooth edge easy to tear; through an integrated three-layer patch structure, by combining the synergistic effect of PLGA microsphere controlled-release oxygen, liposome low-molecular chitosan insulin delivery and gel matrix, the skin injury microenvironment is effectively improved, inflammation is inhibited, and the healing time is shortened.

The invention discloses a medical biological dressing suppository matrix and a preparation method thereof, and relates to the technical field of suppositories, the medical biological dressing suppository matrix is prepared by taking gelatin, glycerol, polyethylene glycol derivatives and chitosan quaternary ammonium salt as raw materials, the biocompatibility of the suppository matrix is good, and stimulation and anaphylactic reaction cannot be generated on a medication cavity; and after the suppository matrix is dissolved in the cavity, a film with good adhesion can be formed on the mucous membrane of the cavity, so that the released medicine is fully contacted with the focus for a long time, the curative effect and bioavailability of the medicine are improved, and the suppository is suitable for preparing suppositories for treating gynecological diseases, anorectal diseases and prostate diseases.

This invention discloses a bio-dress containing multiple anti-aging factors and its preparation method. The bio-dress, by weight percentage, comprises 1-10% collagen solution, 2%-15% styraxine, 0.5%-8% ergothioneine, 1%-12% ectoine, and auxiliary ingredients. This bio-dress integrates multiple anti-aging factors, and through the synergistic effect of these factors, it can effectively improve skin aging problems. It is suitable for medical aesthetics and daily skincare, possessing good anti-aging effects and skin safety.

This invention discloses a medical eye dressing and its preparation method, belonging to the field of medical biological dressing material preparation technology. The method includes the following steps: mixing and stirring graphene oxide-sodium hyaluronate oxide, graphene oxide-trehalose oxide, glycerin, emulsifier, moisturizer, chelating agent, calcium chloride, and deionized water to form a dilute viscous matrix material; filling the dilute viscous matrix material into an aluminum-plastic film bag lined with non-woven fabric and sealing it to obtain the medical eye dressing. This invention directly fills the medical eye dressing into an aluminum-plastic film bag lined with non-woven fabric after mixing and stirring graphene oxide-sodium hyaluronate oxide, graphene oxide-trehalose oxide, glycerin, carbomer, Tween-80, polyglutamic acid, disodium edetate, calcium chloride, and deionized water, thereby improving the breathability of the eye dressing and reducing skin irritation.

The invention relates to a preparation method of a bacterial cellulose composite nano-silver biological dressing, and belongs to the technical field of biological material preparation. Enzymatic hydrolysate of bean pulp, alfalfa leaching liquor and tryptone are used as fermentation culture media, acetobacter xylinum seed liquid is added, the bacterial cellulose membrane is obtained, and the content of the bacterial cellulose membrane composite nano-silver biological dressing is limited to 50-99% of water. Comprising chitosan, gamma-polyglutamic acid, beta-glucan, refined water and silver ions. The bacterial cellulose membrane composite nano-silver biological dressing has the effects that the bacterial cellulose membrane composite nano-silver biological dressing is high in mechanical strength, high in water-retaining property, good in biocompatibility, good in exudate absorption capacity and non-toxic, and as the nano-silver biological dressing for wounds, the cellulose composite nano-silver biological dressing can provide a moist environment, is beneficial to rapid cleaning of the wounds, and has a good application prospect. Meanwhile, the antibacterial activity of chitosan, gamma-polyglutamic acid, beta-glucan, silver ions and the like on bacteria is more beneficial to wound healing.

The invention discloses composite emulsion gel loaded with alpha-linolenic acid as well as a preparation method and application of the composite emulsion gel, and relates to the field of compositions of high-molecular compounds. The preparation method comprises the following steps: firstly, adding alpha-linolenic acid into a soybean protein isolate-tannic acid composite solution, performing high-speed shearing to prepare an emulsion, then adding carrageenan to form emulsion gel, and soaking the emulsion gel into a salt solution to obtain the alpha-linolenic acid loaded composite emulsion gel. Experiments prove that the salt solution can change the structure of the emulsion gel, plays a positive role in improving the loading rate, thermal stability, mechanical strength, oxidation resistance and the like of the gel on an active substance alpha-linolenic acid, and is suitable for making food with high elasticity and harder texture; in addition, the emulsion gel can well protect active substances in the stomach environment, so that the emulsion gel has wide application prospects in the fields of food industry, biological medicine and the like, and high-quality development of the protein-based food and biological dressing industry is further promoted.

The invention discloses a medical eye protection dressing and a preparation method thereof, and belongs to the technical field of medical biological dressing material preparation. Comprising the following steps: mixing and stirring graphene oxide-oxidized sodium hyaluronate, graphene oxide-oxidized trehalose, glycerol, an emulsifier, a humectant, a chelating agent, calcium chloride and deionized water to form a thin mucus matrix material; and filling the thin mucus matrix material into an aluminum-plastic film bag with non-woven fabric, and sealing to obtain the medical eye protection dressing. Graphene oxide-oxidized sodium hyaluronate, graphene oxide-oxidized trehalose, glycerin, carbomer, Tween-80, polyglutamic acid, edetate disodium, calcium chloride and deionized water are mixed and stirred and then directly filled into an aluminum-plastic film bag with non-woven fabric to obtain the medical eye protection dressing, so that the air permeability of the eye protection dressing is improved; the irritation to the skin is relatively low.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

This invention provides a method and application for preparing biological dressings using a combination of enzymes. The preparation method includes static water thawing; dehairing and degreasing; cutting and segmenting; fine trimming and perforation; iodine immersion treatment; purified water immersion treatment; trypsin and neutral protease solution immersion treatment; purified water immersion treatment; antibacterial coating treatment; packaging; and irradiation. This invention significantly improves the antigen removal rate through the combined use of two enzymes. The combined use of these two enzymes enables comprehensive and efficient degradation of different types of heterogeneous proteins, forming a complementary enzymatic process. The combined use of two enzymes can greatly increase the antigen removal rate, significantly improve the biocompatibility of the biological dressing, reduce the risk of immune rejection in clinical applications, and the prepared biological dressing has good biocompatibility and suitable clinical applicability.

The present invention describes a method for obtaining freeze-dried tilapia skin, comprising the steps of cleaning by scraping, washing with physiological saline solution and trimming the edges of the skin; incubation with biocompatible detergent in a sterile recipient and rinsing, incubation with bactericidal agent in sterile recipient and rinsing, incubating with detoxifying solution and rinsing, incubation with antibiotics, rinsing and freezing, cold freeze-drying, vacuum sealing and sterilization, and more specifically the present invention includes the use of tilapia skin to prepare a remedy for treating lesions in humans and animals. The present invention relates to the fields of pharmacy, medicine, dentistry, veterinary medicine, chemistry, biotechnology and tissue engineering.

The utility model relates to the technical field of biological application, and discloses a high-breathability biological application which comprises a non-woven fabric layer, a biological dressing layer, a first binder layer located on the side face of one side of the non-woven fabric layer and located on the peripheral side of the biological dressing layer, and a first release film covering the biological dressing layer. The first release film comprises a first film surface and a second film surface; the first film surface is positioned on one side of the biological dressing layer; the side edge of one side of the first film surface is adhered to the first adhesive layer; the second film surface is positioned on one side, far away from the first film surface, of the biological dressing layer, and the side edge of one side of the second film surface is adhered to the first adhesive layer; an extension surface covering the outer side of the first membrane surface is integrally arranged on the side, away from the first binder layer, of the second membrane surface, elastic bands used for being pressed on the side edges of the non-woven fabric layer are arranged at the two ends of the side face, away from the biological dressing layer, of the non-woven fabric layer, one end of each elastic band is connected with the first membrane surface, and the other end of each elastic band is connected with the second membrane surface; a second binder layer is arranged on the extension surface, and a second release film covers the second binder layer.

The invention provides a biological dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: thawing in still water; unhairing and degreasing; cutting and segmenting; fine trimming and punching; performing iodophor soaking treatment; soaking in purified water; soaking in a mixed solution of protease and alkali metal hydroxide; soaking in purified water; antibacterial agent brushing treatment is carried out; and packaging and irradiating. The product obtained by the invention can be used for purifying, cleaning and protecting wound surfaces, promoting granulation tissue growth, covering granulation wound surfaces, chronic ulcers and bedsores, shortening the healing time and improving the healing effect.

The present application relates to the technical field of biological medicine, in particular to a stem cell film biological dressing for treating skin injury and a preparation method thereof. The present application provides a stem cell film biological dressing, which comprises a composite stem cell film, a hydrogel nutrition layer and an adhesive dressing, wherein the composite stem cell film is formed on one side surface of the hydrogel nutrition layer, the adhesive dressing is formed on the other side of the hydrogel nutrition layer, and the composite stem cell film comprises a plurality of single-layer stem cell films stacked. The stem cell film biological dressing can promote the growth of wound epidermal cells, shorten the wound healing time and shorten the course of disease.

The invention relates to a biological dressing containing glycosylglycerol and a preparation method and application thereof. The biological dressing comprises a base material layer and a functional coating loaded on the base material layer, wherein the functional coating comprises glycosylglycerol (GG), a biocompatible high polymer material and a pH responsive dye; the pH responsive dye can generate macroscopic color change when the pH is increased due to wound infection; the functional coating further comprises mussel mucin and / or epidermal growth factor (EGF), and the functional coating and GG form a sequential controlled release system. The preparation method comprises the step of forming the functional coating through a visible light-initiated'one-step 'cross-linking load or a coaxial electrostatic spinning technology. The dressing disclosed by the invention not only has excellent moisturizing and healing promoting performance, but also can realize early intelligent early warning (pH response) of wound infection and on-demand release (enzyme response) of drugs, and can be used for synergistically resisting bacteria and inflammation, remarkably accelerating the healing process of chronic wound difficult to heal and reducing scar formation.

1. Name of the product of this design: Packaging box (medical polysaccharide biological dressing). 2. Application of this design product: for pharmaceutical packaging. 3. The key design point of this appearance design product lies in the overall shape of the product. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention provides recombinant humanized XVI type collagen and application thereof, and belongs to the technical field of biosynthesis. The recombinant humanized XVI type collagen has excellent cell adhesiveness, biocompatibility and a function of promoting cell viability, and can effectively promote tissue repair and enhance skin barrier. The protein can be used for preparing biological dressings, tissue engineering materials, cosmetic fillers, organoid culture scaffolds, cosmetics and the like, and has wide application prospects in the fields of skin repair, medical cosmetology and tissue engineering.

The invention discloses a preparation method and healing promotion application of a recombinant collagen-chitosan composite biological dressing, and relates to the technical field of biological medicines.The preparation method comprises the steps of modified recombinant collagen preparation, solution blending, step crosslinking, molding and function intensifying. The trehalose and the glycosylglycerol are combined, so that the dressing can keep activity for a long time at a relatively high temperature, meanwhile, the porous three-dimensional bracket structure formed by the gradient freeze-drying process can be combined with the chitosan and the modified collagen, so that the seepage absorption amount is improved, and meanwhile, the absorption capacity of the dressing is improved. The mesoporous structure can be matched with metal ions generated by loading of the nano-silver-mesoporous silicon dioxide suspension to adsorb inflammatory factors in seepage and reduce the risk of secondary infection, and meanwhile, when the magnetic stirrer is used for mixing and stirring materials, the materials can be mixed and stirred by mounting the self-opening and closing stirring device, so that the material mixing efficiency is improved. Therefore, self-coverage mixing anti-splashing and stirring self-power cooperation can be satisfied.

The invention provides recombinant humanized XXVII type collagen and application thereof, and belongs to the technical field of biosynthesis. The recombinant humanized XXVII type collagen has strong cell adhesion and good biocompatibility, can be used for preparing biological dressings, tissue engineering materials, cosmetic fillers, organoid culture scaffolds, cosmetics and the like, and has wide application prospects in the aspects of promoting cell adherence and tissue repair.

The invention discloses a preparation method and application of an extracellular matrix based on human skin organoid. The method comprises the following steps: taking a full-layer human skin tissue, performing enzyme digestion to obtain a single-cell suspension, and inducing in a three-dimensional culture system to form a skin organ with an epidermal layer and corium layer structure; then applying a synergistic effect of physical stimulation and physiological stimulation to the organoid, and efficiently inducing ECM secretion; and finally, carrying out decellularization treatment and ultracentrifugation purification to obtain the high-purity ECM material. The ECM retains a natural three-dimensional network structure, does not contain animal-derived components, and is low in immunogenicity. The prepared ECM has good effects in the aspects of promoting cell adhesion, accelerating wound healing and activating skin cell functions, and can be applied to preparation of biological dressings or anti-aging cosmetics for repairing chronic wounds. The method has the advantages of being short in preparation period, high in structural integrity, good in biological safety, capable of achieving large-scale production and the like.

The invention discloses a modified antibacterial composite biological dressing as well as a preparation method and application thereof, and belongs to the technical field of biological dressings. The biological dressing is formed by compounding a modified antibacterial layer, release paper and an isolating layer, the release paper is adhered to one side of the modified antibacterial layer, and the isolating layer is adhered to the other side of the modified antibacterial layer; the modified antibacterial layer is prepared from chitosan quaternary ammonium salt, sodium alginate, gelatin, a modified double-enzyme carrier and an acetic acid aqueous solution as raw materials; the modified double-enzyme carrier is prepared by aminating SiO2 microspheres and co-immobilizing the aminated SiO2 microspheres with glucose oxidase and horse radish peroxidase. The biological dressing prepared by the invention has good air permeability and antibacterial property, is free of cytotoxicity, and can be applied to postoperative wound care.

The present invention proposes a cell-compounded sheep acellular dermis biological dressing and a preparation method thereof, specifically relating to the technical field of medical biological dressing materials. The cell-compounded sheep acellular dermis biological dressing of the present invention is prepared from sheepskin through depilation, splitting, disinfection, decellularization, freeze-drying, sterilization and co-culturing with cells. It retains the collagen fiber scaffold of the skin and has no cytotoxicity. It can provide a clinical dermis substitute that can carry cells and promote wound healing.

The invention relates to the technical field of biological dressings, in particular to a drug delivery microneedle dressing capable of monitoring pH and a preparation method of the drug delivery microneedle dressing. The preparation method provided by the invention comprises the following steps: adding a photo-thermal material into a mixed solution of hyaluronic acid and polyvinylpyrrolidone to prepare a preformed hydrogel solution, then injecting the preformed hydrogel solution into a polydimethylsiloxane mold, and carrying out centrifugal treatment to enable the hydrogel solution to fully fill a microneedle cavity; then adding the resin microspheres containing the pH indicator into a polyvinyl alcohol solution, and injecting the obtained mixed solution into a mold; and finally, drying the cured microneedle dressing at room temperature for 12 hours to obtain a finished product of the drug delivery microneedle dressing capable of monitoring the pH. The prepared microneedle dressing can monitor the wound healing condition, and meanwhile medicine delivery treatment is achieved.

The utility model discloses a bacterial cellulose biological application which is characterized in that firstly, when a first protective film and a second protective film protect a bacterial cellulose film, buckling pieces on the four sides of the first protective film are mounted in buckling grooves of connecting pieces on the four sides of the first protective film for connection; therefore, the first protective film and the second protective film are connected, the side face of the bacterial cellulose film is limited, and the effect of improving the protective effect is achieved. Meanwhile, in the process that the lifting structure on the first protective film is lifted upwards to drive the first protective film to deform, the buckling piece is separated from the buckling groove, so that the first protective film is convenient to take down, the second protective film is convenient to separate after being held due to the existence of the connecting piece, and the effect of separating the first protective film from the second protective film is achieved.

A method for biosynthesizing type VI collagen as a human structural material is provided. The polypeptide described herein comprises an amino acid sequence or a variant thereof selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20 or 21. The recombinant human type VI collagen herein has cell adhesion activity, is easy to separate and purify, and can be used as a biological dressing, a human bionic material, a plastic surgery material, and the like.

The present application relates to the technical field of biological medicine, in particular to a cell membrane biological dressing for treating skin injury and a preparation method thereof. The present application provides a cell membrane biological dressing, which comprises a fibroblast cell membrane, an epidermal cell membrane, a hydrogel nutrition layer and a breathable dressing. The fibroblast cells and the epidermal cells are prepared into cell membranes, and the cell membranes are combined with the hydrogel nutrition layer to form a novel biological dressing. The cell membranes can be directly applied to the burn skin wound to promote wound healing, and are good skin substitutes.

The invention relates to the technical field of medical supplies, in particular to a double-channel injector. According to the technical scheme, the syringe comprises a rod body, an outer needle cylinder, a rubber plug and an inner needle cylinder, the inner needle cylinder is arranged in the outer needle cylinder, an outer needle head is arranged at the lower end of the outer needle cylinder, an inner needle head inserted into the outer needle head is arranged at the lower end of the inner needle cylinder, the rubber plug is installed in the outer needle cylinder in a sliding mode, and the rod body is arranged on the upper portion of the inner wall of the rubber plug. A rod handle is arranged at the upper end of the rod body. The inner needle cylinder and the outer needle cylinder which are sleeved with each other are used for containing two biological materials at the same time, biological dressing containing two kinds of medicine can be obtained at a time for wound repair, and the functions of reducing the repeated treatment process of a patient and relieving pain of the patient are achieved.