18 results about "Biological dressing" patented technology

The invention relates to the technical field of biology, and particularly discloses polypeptide, recombinant XIX type humanized collagen and application of the recombinant XIX type humanized collagen. The amino acid sequence of the polypeptide comprises n repetitive units; the repetitive unit comprises any one of the following amino acid sequences or variant sequences thereof: (1) an amino acid sequence as shown in any one of SEQ ID NO.1-2; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence as shown in any one of SEQ ID NO.1-2; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in any one of SEQ ID NO.1-2; wherein n is an integer greater than or equal to 1. The polypeptide provided by the invention has a collagen triple helix structure, has good cell adhesion activity, can carry out functions of human collagen, is free of immunological rejection and anaphylactic reaction, and can be used in various fields of cartilage repair, biological dressing, tissue filling and the like.

The invention belongs to the technical field of medical biological dressings, and provides a composite dressing with oxygen release and insulin delivery functions and a preparation method thereof.The composite dressing comprises a supporting base layer, a function slow release layer and a protective film layer which are sequentially compounded from bottom to top; the supporting base layer is a medical porous polyurethane film modified by plasma; the functional sustained release layer is composed of hyaluronic acid-chitosan composite gel, oxygen release microspheres and insulin liposome; the protective film layer is a polyethylene terephthalate film of which the surface is provided with a sawtooth edge easy to tear; through an integrated three-layer patch structure, by combining the synergistic effect of PLGA microsphere controlled-release oxygen, liposome low-molecular chitosan insulin delivery and gel matrix, the skin injury microenvironment is effectively improved, inflammation is inhibited, and the healing time is shortened.

The invention discloses a medical biological dressing suppository matrix and a preparation method thereof, and relates to the technical field of suppositories, the medical biological dressing suppository matrix is prepared by taking gelatin, glycerol, polyethylene glycol derivatives and chitosan quaternary ammonium salt as raw materials, the biocompatibility of the suppository matrix is good, and stimulation and anaphylactic reaction cannot be generated on a medication cavity; and after the suppository matrix is dissolved in the cavity, a film with good adhesion can be formed on the mucous membrane of the cavity, so that the released medicine is fully contacted with the focus for a long time, the curative effect and bioavailability of the medicine are improved, and the suppository is suitable for preparing suppositories for treating gynecological diseases, anorectal diseases and prostate diseases.

This invention discloses a bio-dress containing multiple anti-aging factors and its preparation method. The bio-dress, by weight percentage, comprises 1-10% collagen solution, 2%-15% styraxine, 0.5%-8% ergothioneine, 1%-12% ectoine, and auxiliary ingredients. This bio-dress integrates multiple anti-aging factors, and through the synergistic effect of these factors, it can effectively improve skin aging problems. It is suitable for medical aesthetics and daily skincare, possessing good anti-aging effects and skin safety.

This invention discloses a medical eye dressing and its preparation method, belonging to the field of medical biological dressing material preparation technology. The method includes the following steps: mixing and stirring graphene oxide-sodium hyaluronate oxide, graphene oxide-trehalose oxide, glycerin, emulsifier, moisturizer, chelating agent, calcium chloride, and deionized water to form a dilute viscous matrix material; filling the dilute viscous matrix material into an aluminum-plastic film bag lined with non-woven fabric and sealing it to obtain the medical eye dressing. This invention directly fills the medical eye dressing into an aluminum-plastic film bag lined with non-woven fabric after mixing and stirring graphene oxide-sodium hyaluronate oxide, graphene oxide-trehalose oxide, glycerin, carbomer, Tween-80, polyglutamic acid, disodium edetate, calcium chloride, and deionized water, thereby improving the breathability of the eye dressing and reducing skin irritation.

The invention relates to a preparation method of a bacterial cellulose composite nano-silver biological dressing, and belongs to the technical field of biological material preparation. Enzymatic hydrolysate of bean pulp, alfalfa leaching liquor and tryptone are used as fermentation culture media, acetobacter xylinum seed liquid is added, the bacterial cellulose membrane is obtained, and the content of the bacterial cellulose membrane composite nano-silver biological dressing is limited to 50-99% of water. Comprising chitosan, gamma-polyglutamic acid, beta-glucan, refined water and silver ions. The bacterial cellulose membrane composite nano-silver biological dressing has the effects that the bacterial cellulose membrane composite nano-silver biological dressing is high in mechanical strength, high in water-retaining property, good in biocompatibility, good in exudate absorption capacity and non-toxic, and as the nano-silver biological dressing for wounds, the cellulose composite nano-silver biological dressing can provide a moist environment, is beneficial to rapid cleaning of the wounds, and has a good application prospect. Meanwhile, the antibacterial activity of chitosan, gamma-polyglutamic acid, beta-glucan, silver ions and the like on bacteria is more beneficial to wound healing.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

This invention provides a method and application for preparing biological dressings using a combination of enzymes. The preparation method includes static water thawing; dehairing and degreasing; cutting and segmenting; fine trimming and perforation; iodine immersion treatment; purified water immersion treatment; trypsin and neutral protease solution immersion treatment; purified water immersion treatment; antibacterial coating treatment; packaging; and irradiation. This invention significantly improves the antigen removal rate through the combined use of two enzymes. The combined use of these two enzymes enables comprehensive and efficient degradation of different types of heterogeneous proteins, forming a complementary enzymatic process. The combined use of two enzymes can greatly increase the antigen removal rate, significantly improve the biocompatibility of the biological dressing, reduce the risk of immune rejection in clinical applications, and the prepared biological dressing has good biocompatibility and suitable clinical applicability.

The present invention describes a method for obtaining freeze-dried tilapia skin, comprising the steps of cleaning by scraping, washing with physiological saline solution and trimming the edges of the skin; incubation with biocompatible detergent in a sterile recipient and rinsing, incubation with bactericidal agent in sterile recipient and rinsing, incubating with detoxifying solution and rinsing, incubation with antibiotics, rinsing and freezing, cold freeze-drying, vacuum sealing and sterilization, and more specifically the present invention includes the use of tilapia skin to prepare a remedy for treating lesions in humans and animals. The present invention relates to the fields of pharmacy, medicine, dentistry, veterinary medicine, chemistry, biotechnology and tissue engineering.

The invention provides a biological dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: thawing in still water; unhairing and degreasing; cutting and segmenting; fine trimming and punching; performing iodophor soaking treatment; soaking in purified water; soaking in a mixed solution of protease and alkali metal hydroxide; soaking in purified water; antibacterial agent brushing treatment is carried out; and packaging and irradiating. The product obtained by the invention can be used for purifying, cleaning and protecting wound surfaces, promoting granulation tissue growth, covering granulation wound surfaces, chronic ulcers and bedsores, shortening the healing time and improving the healing effect.

The present application relates to the technical field of biological medicine, in particular to a stem cell film biological dressing for treating skin injury and a preparation method thereof. The present application provides a stem cell film biological dressing, which comprises a composite stem cell film, a hydrogel nutrition layer and an adhesive dressing, wherein the composite stem cell film is formed on one side surface of the hydrogel nutrition layer, the adhesive dressing is formed on the other side of the hydrogel nutrition layer, and the composite stem cell film comprises a plurality of single-layer stem cell films stacked. The stem cell film biological dressing can promote the growth of wound epidermal cells, shorten the wound healing time and shorten the course of disease.

The invention relates to a biological dressing containing glycosylglycerol and a preparation method and application thereof. The biological dressing comprises a base material layer and a functional coating loaded on the base material layer, wherein the functional coating comprises glycosylglycerol (GG), a biocompatible high polymer material and a pH responsive dye; the pH responsive dye can generate macroscopic color change when the pH is increased due to wound infection; the functional coating further comprises mussel mucin and / or epidermal growth factor (EGF), and the functional coating and GG form a sequential controlled release system. The preparation method comprises the step of forming the functional coating through a visible light-initiated'one-step 'cross-linking load or a coaxial electrostatic spinning technology. The dressing disclosed by the invention not only has excellent moisturizing and healing promoting performance, but also can realize early intelligent early warning (pH response) of wound infection and on-demand release (enzyme response) of drugs, and can be used for synergistically resisting bacteria and inflammation, remarkably accelerating the healing process of chronic wound difficult to heal and reducing scar formation.

The invention provides recombinant humanized XVI type collagen and application thereof, and belongs to the technical field of biosynthesis. The recombinant humanized XVI type collagen has excellent cell adhesiveness, biocompatibility and a function of promoting cell viability, and can effectively promote tissue repair and enhance skin barrier. The protein can be used for preparing biological dressings, tissue engineering materials, cosmetic fillers, organoid culture scaffolds, cosmetics and the like, and has wide application prospects in the fields of skin repair, medical cosmetology and tissue engineering.

The invention provides recombinant humanized XXVII type collagen and application thereof, and belongs to the technical field of biosynthesis. The recombinant humanized XXVII type collagen has strong cell adhesion and good biocompatibility, can be used for preparing biological dressings, tissue engineering materials, cosmetic fillers, organoid culture scaffolds, cosmetics and the like, and has wide application prospects in the aspects of promoting cell adherence and tissue repair.

The invention discloses a preparation method and application of an extracellular matrix based on human skin organoid. The method comprises the following steps: taking a full-layer human skin tissue, performing enzyme digestion to obtain a single-cell suspension, and inducing in a three-dimensional culture system to form a skin organ with an epidermal layer and corium layer structure; then applying a synergistic effect of physical stimulation and physiological stimulation to the organoid, and efficiently inducing ECM secretion; and finally, carrying out decellularization treatment and ultracentrifugation purification to obtain the high-purity ECM material. The ECM retains a natural three-dimensional network structure, does not contain animal-derived components, and is low in immunogenicity. The prepared ECM has good effects in the aspects of promoting cell adhesion, accelerating wound healing and activating skin cell functions, and can be applied to preparation of biological dressings or anti-aging cosmetics for repairing chronic wounds. The method has the advantages of being short in preparation period, high in structural integrity, good in biological safety, capable of achieving large-scale production and the like.

The invention relates to the technical field of biological dressings, in particular to a drug delivery microneedle dressing capable of monitoring pH and a preparation method of the drug delivery microneedle dressing. The preparation method provided by the invention comprises the following steps: adding a photo-thermal material into a mixed solution of hyaluronic acid and polyvinylpyrrolidone to prepare a preformed hydrogel solution, then injecting the preformed hydrogel solution into a polydimethylsiloxane mold, and carrying out centrifugal treatment to enable the hydrogel solution to fully fill a microneedle cavity; then adding the resin microspheres containing the pH indicator into a polyvinyl alcohol solution, and injecting the obtained mixed solution into a mold; and finally, drying the cured microneedle dressing at room temperature for 12 hours to obtain a finished product of the drug delivery microneedle dressing capable of monitoring the pH. The prepared microneedle dressing can monitor the wound healing condition, and meanwhile medicine delivery treatment is achieved.

The present application relates to the technical field of biological medicine, in particular to a cell membrane biological dressing for treating skin injury and a preparation method thereof. The present application provides a cell membrane biological dressing, which comprises a fibroblast cell membrane, an epidermal cell membrane, a hydrogel nutrition layer and a breathable dressing. The fibroblast cells and the epidermal cells are prepared into cell membranes, and the cell membranes are combined with the hydrogel nutrition layer to form a novel biological dressing. The cell membranes can be directly applied to the burn skin wound to promote wound healing, and are good skin substitutes.

The present application relates to the technical field of biotechnology, and particularly discloses a polypeptide, a recombinant humanized collagen of type XIX and application thereof. The amino acid sequence of the polypeptide comprises n repeating units; the repeating unit comprises any one of the following amino acid sequences or a variant sequence thereof: (1) an amino acid sequence shown in any one of SEQ ID NO. 1-2; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence shown in any one of SEQ ID NO. 1-2; (3) a variant sequence obtained by mutating one or more amino acid residues in the amino acid sequence shown in any one of SEQ ID NO. 1-2; wherein n is an integer greater than or equal to 1. The polypeptide has a collagen triple helix structure, has good cell adhesion activity, can perform the function of human collagen, has no immune rejection and allergic reaction, and can be used in various fields such as cartilage repair, biological dressing and tissue filling.