82 results about "Nerve repair" patented technology

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

According to the invention, a bicontinuous phase conductive core-shell nanofiber membrane is applied to construction of a flexible neural electrode and a neural conduit, firstly, a bicontinuous phase conductive polymer system is constructed, and the system realizes a three-dimensional network in which an electric phase and a mechanical phase are respectively continuous, interpenetrating but independent; and the long-term technical problems of conductive component agglomeration, conductive path interruption and the like are effectively solved. And preparing the conductive nanofiber scaffold with a core-shell structure by taking a high-molecular polymer as a core layer material and a bicontinuous-phase conductive polymer system as a shell layer material through a coaxial electrostatic spinning method. The construction mode effectively establishes a stable, continuous and uniform electron conduction path, can realize effective nerve electrical stimulation and high-fidelity electrophysiological signal recording in vivo, and can be used as a nerve conduit to regulate and repair related bio-electricity signals in a nerve repair process, so as to accelerate regeneration and repair of nerves.

The present disclosure pertains to membranous tissue grafts comprising one or more pre-made attachment points. The one or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. In some embodiments, the membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, methods are provided for repairing a damaged or severed nerve by implanting the membranous tissue grafts on to the damaged or severed nerve.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The invention discloses an active composite gel sealing material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The active composite gel sealing material based on multiple crosslinking provided by the invention has excellent tissue adhesion and sealing performance; good in-vivo compatibility, biodegradability, low immunogenicity and high safety are achieved; the mechanical strength is high, the self-repairing capability is good, and the anti-hydrolysis stability is high; the adhesive property and the state of the material can be adjusted by adjusting the proportion of the components and adjusting the adhesive property and the state of the material, so that the requirements on different adhesive properties in practical application are met, irregular wounds are filled, and rapid gelation is performed at the body temperature so as to adapt to the form of a surgical site; the effects of stopping bleeding, resisting inflammation, promoting revascularization and repairing nerves are also achieved.

The application belongs to the field of medical biological materials, and relates to a preparation method of a hierarchical nanofiber composite film for nerve repair and application thereof. The method comprises the following steps: preparing multilayer nanofibers with different internal stresses through continuous multiple electrostatic spinning units, stacking the multilayer nanofibers with different internal stresses to form a composite nanofiber film with an internal stress gradient; chemically crosslinking the composite nanofiber film; performing adhesion molecule adhesion treatment on the upper surface of the composite nanofiber film; and preparing the hierarchical nanofiber composite film with an internal stress gradient and capable of self-deforming under the action of liquid to wrap nerves. The film provided by the application has excellent flexibility, biocompatibility and degradation performance, can efficiently and quickly adhere to the surface of damaged nerves, provides a good support microenvironment for nerve cell growth, promotes nerve regeneration and repair, and has a wide application prospect in the field of peripheral nerve injury repair.

The invention relates to the technical field of biomedical materials, in particular to a high-conductivity peripheral nerve socketed pipe and a preparation method thereof. The nerve socketed pipe is a hollow cylindrical socketed pipe made of a material containing poly (octylene-citrate-co-octanol) POCO, and a regeneration promoting material is loaded in the hollow cylindrical socketed pipe; the regeneration promoting material comprises fetal calf serum and a DMEM cell culture medium. The POCO has the advantages of conductive activity, nerve regeneration induction, free radical scavenging ability, oxidation resistance, deformation resistance and the like, and has good degradability and biocompatibility, so that the nerve socketed pipe is prepared from poly (octylene-citrate-co-octanol), and the growth of axons is accelerated through electrical stimulation. Furthermore, at least one conductive monomer of pyrrole, aniline, p-phenylenediamine, o-methoxyaniline, thiophene, 3-hexylthiophene, 3-thiopheneacetic acid and 3, 4-ethylenedioxythiophene is adopted for modifying the POCO hollow cylindrical socketed pipe through in-situ oxidative polymerization, and the performance of the POCO hollow cylindrical socketed pipe serving as the neural restoration material is further improved.

The invention relates to the technical field of curative effect prediction, in particular to a curative effect prediction method and system for treating spinal cord injury by stem cells, and the method comprises the following steps: obtaining a multi-parameter metabolism sequence and constructing an activation rhythm map, recognizing a neural restoration synchronization section, extracting a metabolism dominant path and evaluating electrical signal response, generating a para-position map layer and predicting a curative effect trend. According to the method, through introduction of cross-time-sequence metabolism and trend synchronous identification of electrophysiological indexes, dynamic mapping of stem cell activation rhythms is realized, neural axon morphological changes and cerebrospinal fluid metabolism parameters are linked, and distribution positions of key response points in a treatment cycle are determined; the method comprises the following steps: establishing a causal path based on a sequence of metabolic mutation and neural restoration events, extracting an evolution stage dominated by a metabolic driving effect, marking a matching trend of a curative effect response and an intervention path on a time axis, identifying the number and positions of trend convergence, withdrawal and interruption areas, and realizing collaborative prediction of a restoration progress and a curative effect trend.

The scheme provides a kind of engineered exosome double drug delivery system loaded with ergosterol and its preparation method and application, belongs to the field of nanomaterials and nanobiomedicine, based on the innovative ROS-responsive nanomaterial CHPG design, load the active ingredient ergosterol to play the role of treatment and repair, which realizes the blood-brain barrier penetration and precise drug release in lesion area through targeted functional modification;It is wrapped in the nasal mucosa-derived stem cell exosome to form a drug delivery system Exo@Erg-CHPG-M, which combines the natural biological characteristics of exosome and the precise targeting advantage of nanomicelle, significantly improves the drug delivery efficiency, in vitro and animal model verification show that, the system can improve the neurological impairment and brain tissue injury after ischemic stroke by synergistically regulating inflammation and neural repair pathways, the scheme provides a new technical scheme for the precise treatment of central nervous system diseases such as cerebral stroke.

The conduit carrier includes a carrier housing that includes first and second contact ends and a vacuum channel extending between them. The vacuum channel is configured to connect to a vacuum source via the first contact end. An engagement mechanism is connected to the second contact end. The engagement mechanism is configured to engage with the conduit in a releasable manner during nerve repair surgery. A separator is configured to extend from the second contact end. When the engagement mechanism engages with the conduit during nerve repair surgery, the vacuum channel is connected to a vacuum port in the conduit via the second contact end so that a vacuum source can draw a vacuum into the conduit, and the separator extends into the conduit through the vacuum port, creating a predetermined regeneration gap between the first and second nerve stumps inserted into the conduit.

The invention relates to a degradable nerve repair membrane and a preparation method thereof, and belongs to the technical field of nerve repair membranes, the degradable nerve repair membrane comprises a biological composite structure inner layer, a support outer layer and a bonding layer, and the biological composite structure inner layer and the support outer layer are bonded through the bonding layer. According to the invention, polypyrrole with excellent electrochemical activity, environmental stability, controllable conductivity and poor mechanical properties and collagen nanofibers with high biocompatibility and capable of forming a nano-scale fiber network are adopted to form a conductive-biological composite structure as an inner layer through electrostatic spinning, and the PLGA porous membrane is prepared through 3D printing. A PLGA porous membrane with parallel axial microchannels on the surface is used as an outer layer, so that the membrane body structure can effectively guide directional growth of axons while ensuring conductivity, accurate preparation can be realized according to needs, the degradation period is matched with the nerve regeneration rate, and the degradation time can be regulated and controlled through the proportion of a copolymer; the repairing effect is prevented from being influenced by too fast or too slow degradation.

The invention relates to application of graphene-(RADA-IKVAV)-pranoprofen, eye drops and a preparation method of the eye drops. The invention relates to graphene-(RADA-IKVAV)-pranoprofen, the graphene-(RADA-IKVAV)-pranoprofen comprises graphene-polyacrylic acid, self-assembled oligopeptide RADA-IKVAV and pranoprofen, the self-assembled oligopeptide RADA-IKVAV is connected with the graphene-polyacrylic acid through a chemical bond, and the pranoprofen is loaded on the surface of the graphene-polyacrylic acid. The eye drops provided by the invention comprise graphene-(RADA-IKVAV)-pranoprofen, so that the drug loading capacity of pranoprofen in the eye drops is increased, the bioavailability of drugs is improved, and the eye drops are used for inhibiting eye inflammation; the coupled oligopeptide RADA-IKVAV simulates an extracellular environment, provides a suitable microenvironment for eyes, promotes nerve repair and regeneration by influencing nerve growth factors, and promotes optic nerve repair.

This invention relates to the field of biomedical materials technology, specifically disclosing a hydrogel material for spinal cord nerve repair containing magnetic nanospheres and its preparation method. The hydrogel material provided by this invention is a photocurable hydrogel formulated using chitosan, dimethylaminoethyl methacrylate, methacryloyloxyethyltrimethylammonium chloride, and magnetic iron oxide nanospheres. Compared with traditional spinal cord nerve repair materials, the hydrogel material provided by this invention has good biocompatibility, is safe and non-toxic, and has a simple and easy preparation method. Photocuring is performed when the hydrogel is injected into the spinal cord injury site, while a magnetic field is simultaneously applied to the injury site. This causes the magnetic nanospheres to rotate according to the magnetic field, forming a series of magnetic channels. As the hydrogel gradually solidifies, the magnetic channels are permanently retained. During the spinal cord nerve repair process, the magnetic channels guide nerve growth, preventing incorrect nerve connections and ensuring the effectiveness of nerve repair.

The invention provides a sequential slow-release hydrogen sulfide nerve repair conduit and a preparation method thereof, and belongs to the technical field of biological materials. The sequential slow-release hydrogen sulfide nerve repair conduit comprises degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel and a pH response hydrogen sulfide donor, wherein the degradable polymer conduit is filled with the degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, and the pH response hydrogen sulfide donor is loaded on the hydrogel. In the application, the pH response donor can respond to an acidic microenvironment of degradation of polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, release hydrogen sulfide and relieve the acidic environment generated by degradation of the hydrogel at the same time, and form a dual supply mechanism with cysteine (endogenous hydrogen sulfide precursor) obtained by degradation of the hydrogel; nerve regeneration and angiogenesis are synergistically promoted, and oxidative stress is regulated; and the hydrogel is accelerated to degrade during excessive oxidative stress, so that hydrogen sulfide is released as required, and the core problems of insufficient blood supply, unbalanced acidic microenvironment and slow nerve regeneration are synchronously solved.

The present invention provides tissue grafts having pre-fabricated attachment points (mounting points). [Solution] One or more pre-fabricated attachment points include pre-fabricated markings and / or pre-fabricated suture holes. The membrane tissue graft may be in the shape of a tube. Alternatively, the membrane tissue graft may be in the shape of a rectangle and can be used in nerve repair by wrapping around a severed or damaged nerve. The membrane tissue graft is suitable for repairing severed nerves that have a short gap of less than 5 mm between the severed ends, or nerves that do not have a gap. Therefore, a damaged or severed nerve can be repaired by implanting a membrane tissue graft onto it.

The invention provides a traditional Chinese medicine compound composition with a nerve repairing effect, a cell culture medium, a traditional Chinese medicine-neural stem cell exosome compound and application, and belongs to the technical field of biological medicine. Based on the basic theory of traditional Chinese medicine and the combined theory of traditional Chinese medicine and western medicine, the traditional Chinese medicine compound composition is provided and comprises gastrodia elata exosomes, astragalus membranaceus exosomes and resveratrol, the traditional Chinese medicine compound composition is prepared into a cell culture medium, neural stem cells are cultured through the cell culture medium, and the neural stem cells are obtained. Therefore, the traditional Chinese medicine-neural stem cell exosome compound with an efficient nerve repairing function is prepared. The traditional Chinese medicine-neural stem cell exosome compound achieves the purpose of comprehensively repairing nerve injury by precisely targeting nerve cells, promoting neurogenesis, repairing the function of a neural circuit and reducing the inflammatory effect, and can be used for preparing related medical instruments and drugs for nerve repair.

The application provides a nano drug delivery system based on a Gap19 polypeptide. The system is prepared by mixing DSPE-PEG2k-Gap19 and ROS response material DSPE-TK-PEG5k-MPC to form nanoparticles, has a double-layer structure, and can enhance the stability and blood-spinal cord barrier penetration capacity of Gap19. The ROS response characteristics can realize damage microenvironment triggered drug release, simultaneously remove active oxygen and inhibit Cx43 hemichannel activity, and synergistically promote nerve repair. Experiments show that the system has good biocompatibility, targeted aggregation and functional recovery effect, and provides a new material and strategy for spinal cord injury treatment.

The invention relates to a spinal cord injury repair method based on four-dimensional multifunctional hydrogel, which comprises the following steps: firstly, encapsulating NT-3 in PLGA (poly (lactic-co-glycolic acid)) microspheres through a microsphere technology, and integrating HCT (Hydroxycycline Computed Tomography) into SilMA hydrogel, so as to construct four-dimensional multifunctional hydrogel 4DMSH with time specificity; the hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes eNSCs to differentiate into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved; the four-dimensional multifunctional hydrogel has time specificity and can create an anti-inflammatory and neuroactive microenvironment, so that effective spinal cord injury repair and nerve regeneration are realized; the hydrogel can be widely applied to SCI treatment, and a brand new strategy is provided for repairing nervous system injury.

The application discloses a carrier for engineering miR-124-3p The application discloses an application of an exosome hydrogel in preparation of a drug for treating traumatic brain injury, and belongs to the technical field of biological carrier drug delivery. miR-124- 3p The exosome vesicle structure can improve the stability of the miRNA in the body and the delivery efficiency of the miRNA into nerve cells, thereby enhancing the bioavailability. The engineering exosome is embedded in the QCC / OHM hydrogel system, so that the engineering exosome forms a stable delivery platform at the injury site, realizes sustained release and local enrichment of the exosome. The hydrogel material has good tissue adaptability, can enter irregular brain injury interstitial spaces through minimally invasive injection and form a scaffold structure, and provides a favorable microenvironment for nerve repair. The application can overcome the problems of poor stability of treatment molecules, short local retention time and difficulty in realizing multi-mechanism synergistic treatment, and provides a new technical scheme for treatment of traumatic brain injury.

The utility model discloses a nerve repair suture assist device which comprises a transparent plate, a mounting plate is fixedly connected to the side wall of the transparent plate, and a limiting block is connected to the side wall of the mounting plate in a sliding mode. The damping rod fixes the position of the first magnetic plate, the magnetism of the first magnetic plate is opposite to that of the second magnetic plate, and when the first magnetic plate is installed at the upper end of the second magnetic plate through the damping rod, the second magnetic plate moves downwards through the magnetic repulsion effect between the first magnetic plate and the second magnetic plate to extrude the spring; meanwhile, a sliding rod descends, the sliding rod is inserted into limiting holes in different positions to limit a moving column, then a limiting block is limited, the situation that the limiting block moves at will in the using process of the device is prevented, meanwhile, an observation mirror is installed through a metal shaping hose, and the position of the observation mirror is convenient to adjust.

The invention provides an injectable porous microsphere mesenchymal stem cell compound as well as a preparation method and application thereof, and relates to the field of biomedical materials and regenerative medicine. The injectable porous microsphere mesenchymal stem cell compound comprises a carrier and mesenchymal stem cells loaded on the carrier, the carrier is dMBG / PLGA porous microspheres, the dMBG / PLGA porous microspheres are prepared from dMBG and PLGA, the dMBG is dendritic mesoporous bioactive glass, and the PLGA is a polylactic acid-glycolic acid copolymer; the mesenchymal stem cells enter the three-dimensional through hole channels of the carrier and are planted in the three-dimensional through hole channels. According to the injectable porous microsphere mesenchymal stem cell compound, efficient planting and local retention of MSC can be achieved, high activity of cells after injection and cryopreservation resuscitation is guaranteed, and endogenous osteogenesis can be promoted without exogenous factors; meanwhile, the system has the functions of promoting angiogenesis, regulating immunity and repairing nerves, can fill irregular defects in a minimally invasive mode, can be applied in a large scale, is adaptive to various repairing scenes, and meets the clinical application requirements.

The invention provides a composite active dressing for neural restoration and a preparation method and application thereof, and relates to the technical field of medical materials. The composite active dressing comprises a transdermal inner layer and a small extracellular vesicle slow-release outer layer, the transdermal inner layer is of a membrane-shaped structure and comprises a composite active component and a hydrogel matrix material, and the composite active component is selected from one or more of an anti-inflammatory component, a microcirculation improving component, an antioxidant component and a neurotrophic component; the small extracellular vesicle sustained-release outer layer is of a three-dimensional network structure and comprises small extracellular vesicles and a hydrogel matrix material. According to the invention, stable loading and layered deep delivery and release of the small extracellular vesicles are realized, the inflammatory response and microcirculation state of an injured part are improved, proliferation migration and remyelination of Schwann cells can be promoted, and axon regeneration and effective repair of nerve injury can also be promoted.

The application discloses a waterproof and breathable type brain and spinal cord nerve injury wound protection dressing, which is sequentially stacked and arranged from inside to outside as a wound adhesion inner layer, a functional sustained-release middle layer and a waterproof and breathable outer layer; the three-layer synergistic dressing structure of the wound adhesion inner layer, the functional sustained-release middle layer and the waterproof and breathable outer layer is constructed, waterproof sealing and the dynamic adaptation of the air permeability performance of the brain and spinal cord nerve injury wound are realized, the target anti-scar polypeptide is covalently bonded on the inner layer, the effective inhibition of excessive scar hyperplasia and neuroma formation is realized, the matching regulation of the gradient degradation drug-loaded microsphere system and the air permeation pore size of the middle layer is realized, the accurate release of active factors in the whole cycle of nerve repair is realized, the long-acting waterproof and breathable protection in a dynamic scene is realized through the design of the outer layer of super-hydrophobic nanofiber membrane, the risk of postoperative complications is reduced, and the integration and synergy of the physical protection of the wound and the nerve function repair are realized.

The application belongs to the technical field of nerve grafts, and discloses a multilayer composite capacitive film, a preparation method and application of the multilayer composite capacitive film in nerve repair scaffolds. The scaffold is composed of three layers, namely, an A layer, a B layer and an A layer. The A layer is a sandwiched layer on both sides, and comprises a mixture of chitosan (or silk fibroin or a decellularized matrix) and a conductive polymer PEDOT:PSS and a crosslinking agent polyethylene glycol diglycidyl ether (PEGDE). The B layer is a middle hydrogel sandwiched layer, and comprises methacrylated gelatin (Gel-MA). The A layers on both sides are bonded through the B layer to form a viscoelastic hydrogel after ultraviolet light irradiation, and the composite capacitive scaffold in a multilayer structure with a through middle cavity is obtained after curing and air drying. The formed scaffold has good electrical conductivity, mechanical properties and biological safety, and can effectively induce the proliferation, migration and differentiation of RSC96 Schwann cells and nerve system cells PC12, thereby providing a good physical stable support and a favorable microenvironment for peripheral nerve injury repair.

The invention discloses hydrogel containing 7-amino acid phosphorylated peptide and application of the hydrogel in neural restoration. The hydrogel comprises acellular matrix hydrogel, 7-amino acid phosphorylated peptide, 7-amino acid phosphorylated peptide, 7-amino acid phosphorylated peptide and 7-amino acid phosphorylated peptide, and a 7-amino acid phosphorylated peptide supported in the acellular matrix hydrogel. The 2, 7-amino acid phosphorylated peptide can play a multi-dimensional regulation role in nerve injury microenvironment remodeling, realizes dynamic targeted regulation of peripheral nerve injury microenvironment, and promotes rapid regeneration and recovery of injured peripheral nerves.

The invention belongs to the technical field of nerve targeted therapy, and discloses a nerve targeted spinule drug penetration integrated therapy method for cervical and lumbar spondylosis, which comprises the following steps: determining a lesion segment through image or electrophysiology positioning, applying a soluble spinule patch with layered design in a target area, and rapidly releasing a surface drug to relieve pain. The inner-layer drug carrier is gradually released to play anti-inflammatory and nerve repairing roles; the surface of the carrier can modify a nerve targeting ligand to improve local concentration. The method can also be combined with a low-frequency or high-frequency electrical stimulation module to enhance drug permeation and neurological function recovery, and matched with a sensor to monitor skin impedance or temperature in real time so as to ensure safety. The invention has the advantages of rapid analgesia, long-acting maintenance and high targeting, avoids the defects of oral administration and injection treatment, and has clinical application values of minimally invasive, individualized and suitable for long-term management.

The invention discloses a preparation method of a bio-based neural restoration cannula material, and relates to the field of biological materials. The preparation method comprises the following steps: firstly, enabling chitosan, 3-carboxyphenylboronic acid and maleic anhydride to react with nanogold to prepare modified chitosan; acrylic acid, acrylic acid-N-hydroxysuccinimide, alpha-ketoglutaric acid, modified chitosan and glutaraldehyde are subjected to a reaction, hydrogel is prepared, insulin-like growth factor-1 is added, and the bio-based neural restoration cannula is prepared. The prepared bio-based nerve repair sleeve has excellent biocompatibility, antibacterial property and mechanical property, nerves can be effectively repaired without suture, and secondary damage to nerve broken ends is avoided.

A spinal cord injury repair method based on biphase fibroin and methacryloyl hydrogel comprises the following steps: firstly, encapsulating NT-3 and NSCs in PLGA microspheres through a microsphere technology, and integrating Ang-(1-7) into SilMA hydrogel, so as to construct the biphase SilMA multifunctional hydrogel (DPSH) with time-specific drug controlled release. The hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes NSCs to be differentiated into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved.

This invention provides a modular injectable dynamic hydrogel, its preparation method, and its applications, belonging to the field of medical preparation technology. By designing specific preparation schemes and combining different functional modules, this invention successfully obtained a series of injectable dynamic hydrogels with tunable performance. These hydrogels generally possess good injectability and self-healing capabilities, ensuring their convenience in minimally invasive surgery and structural integrity after implantation. Simultaneously, by adjusting the types and ratios of modules, the degradation rate, cell adhesion, and specific biological functions of the hydrogel can be precisely controlled, all exhibiting excellent biocompatibility and reactive oxygen species scavenging capabilities. This invention broadens the applicability of single hydrogel systems, enabling them to flexibly adapt to various differentiated medical scenarios, from drug controlled release and nerve repair to tissue regeneration, providing an efficient platform for the development of personalized and functional biomaterials.