The invention relates to a two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and a preparing method thereof. Firstly, a parallel porous polylactic acid filament membrane with the diameter ranging from 50 nm to 2000 nm is put into a mixed aqueous solution containing sodium alkyl sulfonate, polyglutamic acid, FeCl3 and pyrrole, then a sufficient reaction is carried out for 2-24 h with an in-situ polymerization method, the surface of the parallel filament membrane is evenly coated with polypyrrole nano-particles, washing and drying are carried out, and then conductive polypyrrole wrapped polylactic acid fiber filament membrane with the diameter ranging from 60 nm to 2500 nm is obtained; then, protein of the two nerve factors, namely, NGF and NT-3, is connected to the parallel conductive porous composite filament membrane in sequence with the carbodiimide technology, and the conducive composite filament membrane which can release nerve factors and promote nerve axon elongation and peripheral nerve repair under electrical field stimulation is obtained. The two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and the preparing method thereof have the advantages that implementation is easy, the nerve factor connection efficiency is high, the material conductivity is high, and the nerve repair time is short.