209 results about "Nerve repair" patented technology

The invention relates to a pathologically responsive nano-carrier as well as a preparation method and application thereof, belongs to the technical field of biological medicines, and aims to solve the problems that in the prior art, nano-particles are enriched in livers and kidneys in quantity, the risk of damaging organs and tissues exists, toxic and side effects on patients with spinal cord injury are large, the treatment effect is not obvious and the like. The carrier is a self-assembled nanoparticle which takes PLGA as a skeleton structure, is externally modified with hyaluronic acid and is connected with RVG29 through a diselenide bond. The carrier can load an active substance, such as curcumin, can be used for treating spinal cord injury, and can target spinal cord tissues through intravenous administration, penetrate a blood spinal cord barrier, target CD44 through HA at the early stage of injury, increase immune cell uptake and inhibit inflammatory infiltration and inflammatory cascade. In the late stage of injury, the nerve cell uptake is increased through RVG29 targeted acetylcholine receptor (nAchR), and nerve repair and regeneration are promoted.

Disclosed herein are non-woven graft materials for use in specialized surgical procedures involving nerve repair and regeneration. Some embodiments describe electrospun fiber products such as conduits, wraps, or grafts comprising a resorbable hybrid-scale matrix to facilitate nerve repair and regeneration.

The invention discloses an extracellular matrix-imitated anti-inflammatory nerve repair-promoting conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of high polymer materials. The preparation method of the conductive hydrogel comprises the following steps: preparing an MXene nanosheet dispersion liquid, and sequentially adding polyphenol and magnesium ions into the MXene nanosheet dispersion liquid to obtain a modified nanosheet solution; mixing the modified chitosan with polyvinyl alcohol to obtain a solution A; mixing and reacting the modified nanosheet solution with phenylboronic acid modified gelatin to obtain a solution B; mixing the solution A and the solution B for reaction, refrigerating at low temperature, and unfreezing to obtain the conductive hydrogel. The hydrogel prepared by the invention has excellent conductivity, and the obtained conductive hydrogel has the functions of resisting inflammation and promoting nerve repair. The bionics method not only improves the diversity and functionality of biological materials, but also provides new possibility for future tissue engineering and regenerative medicine.

The invention provides a fabric-reinforced electroactive nerve conduit and a preparation method and application thereof, and belongs to the field of biomedical materials.The fabric-reinforced electroactive nerve conduit comprises a nerve conduit inner layer and a nerve conduit outer layer, the nerve conduit inner layer is a woven skeleton formed by magnesium wires serving as parallel yarns and degummed silk through a three-dimensional weaving process, the pore diameter of the nerve conduit inner layer is 1.5-2 mm, and the thickness of the nerve conduit inner layer is 0.5-1 mm; the outer layer of the nerve conduit is a composite layer with a radial finger-shaped porous structure, which is formed by directionally freezing and casting a hydrogel cross-linked network formed by mixing potassium sodium niobate nanorods modified by polydopamine and a biopolymer material, and the thickness of the outer layer of the nerve conduit is 1-2mm. A three-dimensional weaving technology, a piezoelectric nano material and a biopolymer material are combined, a novel multifunctional nerve conduit is developed to repair nerve injury, a better solution is provided for nerve repair, and the fabric enhanced electric activity nerve conduit has important practical guiding significance in application of future clinical nerve injury repair.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention discloses a functional polypeptide for promoting repair of endometrial stem cells to clitoris sensitive nerves and application, and belongs to the technical field of stem cell repair, the functional polypeptide is used for modifying the endometrial stem cells and Schwann cells through the functional polypeptide, and the clitoris sensitive nerves are repaired in a local slow-release gel delivery mode. The functional polypeptide can activate Laminin expression and regulate and control ECM degradation balance, so that collagen deposition in scar tissues is reduced; in addition, the functional polypeptide can effectively inhibit HOXA1 expression, enhance the axon extension capacity and strengthen the axon microtubulin polymerization capacity of the nerve injury part, and the nerve repair efficiency and the function recovery quality are remarkably improved.

The invention discloses a pre-stretched hydrogel nerve conduit for promoting directional growth of nerves and a preparation method of the pre-stretched hydrogel nerve conduit, and belongs to the field of preparation of multifunctional hydrogel biomedical materials. The preparation method of the nerve conduit comprises the following preparation steps: preparing a polyvinyl alcohol solution, adding gelatin into the polyvinyl alcohol solution, stirring to enable the gelatin to be completely dissolved in the polyvinyl alcohol solution to prepare a mixed solution, putting the mixed solution into a mold, gelatinizing for a certain time, and performing circulating freezing-melting cross-linking to obtain the nerve conduit. And soaking in a sodium chloride / borax solution, and circularly stretching the hydrogel to obtain the pre-stretched hydrogel nerve conduit. The inside of the pre-stretched hydrogel nerve conduit has a three-dimensional network porous structure, and the inside of the pre-stretched hydrogel nerve conduit is of a porous structure, so that nutrient substance transfer and metabolite discharge in the nerve repair process are facilitated. The surface of the pre-stretched hydrogel nerve conduit is provided with a topological structure for promoting growth of nerve cells, and the nerve cells are guided to grow directionally in the repairing process. Besides, the pre-stretched hydrogel nerve conduit has excellent biocompatibility and adjustable mechanical properties, can be used for bridging operations after peripheral nerve injury, and is gradually degraded and absorbed by organisms in the nerve regeneration process. The nerve conduit provided by the invention shows wide application potential in the field of biomedicine, and can efficiently promote repair and regeneration of human tissues such as nerves and blood vessels.

The invention discloses a bipeptide modified bionic nano-vesicle as well as a preparation method and application thereof, and belongs to the field of biological medicines. The dipeptide modified bionic nano-vesicle comprises nano-particles formed by PLGA (poly (lactic-co-glycolic acid)), and the nano-particles are loaded with a medicine with a nerve protection or nerve repair effect; the surface of the nanoparticle is coated with a macrophage membrane for expressing RVG peptide and T7 peptide. The bipeptide modified bionic nano-vesicle simultaneously presents T7 peptide and RVG peptide through an engineered macrophage membrane, the T7 peptide is combined with a blood-brain barrier transferrin receptor through high affinity to realize efficient brain entry, astrocytes in the brain are specifically recognized by virtue of the RVG peptide, accurate recognition and delivery of target cells in a focus area are realized, and the bipeptide modified bionic nano-vesicle has a good application prospect. Meanwhile, the natural inflammation tropism and immune escape ability of a macrophage membrane are reserved, and the problems that a traditional drug delivery system is low in targeting precision, and cross-barrier distribution and intracerebral distribution are difficult to cooperate are solved.

The invention provides a self-assembled temperature-sensitive hydrogel for neural restoration and a preparation method thereof. The hydrogel comprises the following raw materials: poloxamer, an acellular matrix and a neural factor in a mass concentration ratio of (5-10): (10-20): (1-4), preferably 5: 15: 1. The preparation method comprises the following steps: respectively preparing a poloxamer solution and an acellular matrix solution, mixing and heating the poloxamer solution and the acellular matrix solution to form a double-network three-dimensional gel structure, adding the nerve factors, stirring or ultrasonically dispersing, and heating to form the bioactive hydrogel. The hydrogel can rapidly form a stable three-dimensional structure at the body temperature, a support is provided for nerve repair, cell adhesion and proliferation are supported, and the added nerve factors are beneficial to maintaining the activity of neurons. Meanwhile, the hydrogel is in a liquid state at a low temperature, is convenient to inject, is fixed at a body temperature, is adjustable in degradation rate, is non-toxic, is suitable for local nerve injury treatment, and improves the bioavailability and safety of a nerve repair material.

According to the invention, a bicontinuous phase conductive core-shell nanofiber membrane is applied to construction of a flexible neural electrode and a neural conduit, firstly, a bicontinuous phase conductive polymer system is constructed, and the system realizes a three-dimensional network in which an electric phase and a mechanical phase are respectively continuous, interpenetrating but independent; and the long-term technical problems of conductive component agglomeration, conductive path interruption and the like are effectively solved. And preparing the conductive nanofiber scaffold with a core-shell structure by taking a high-molecular polymer as a core layer material and a bicontinuous-phase conductive polymer system as a shell layer material through a coaxial electrostatic spinning method. The construction mode effectively establishes a stable, continuous and uniform electron conduction path, can realize effective nerve electrical stimulation and high-fidelity electrophysiological signal recording in vivo, and can be used as a nerve conduit to regulate and repair related bio-electricity signals in a nerve repair process, so as to accelerate regeneration and repair of nerves.

The present disclosure pertains to membranous tissue grafts comprising one or more pre-made attachment points. The one or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. In some embodiments, the membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, methods are provided for repairing a damaged or severed nerve by implanting the membranous tissue grafts on to the damaged or severed nerve.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The invention provides a piezoelectric dressing for promoting nerve repair of a diabetic wound and a preparation method and application of the piezoelectric dressing, and belongs to the technical field of piezoelectric dressings. The preparation method comprises the following steps: firstly, respectively preparing a polymer solution and a conductive polymer dispersion liquid, then mixing the polymer solution and the conductive polymer dispersion liquid, carrying out secondary stirring to obtain an electrostatic spinning solution, and then carrying out electrostatic spinning and post-treatment in sequence to obtain the piezoelectric dressing for promoting neural restoration of the diabetic wound. By optimizing the ratio of the raw materials and cooperating with an electrostatic spinning process, triple functions of high-strength piezoelectric response, peripheral nerve repair and macrophage polarization regulation are realized, and the separation limitation of the prior art on nerve repair and immune microenvironment dynamic regulation is broken through; and an integrated solution of electrical stimulation, nerve regeneration, immune regulation and wound healing is provided for the diabetic wound.

The invention discloses an active composite gel sealing material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The active composite gel sealing material based on multiple crosslinking provided by the invention has excellent tissue adhesion and sealing performance; good in-vivo compatibility, biodegradability, low immunogenicity and high safety are achieved; the mechanical strength is high, the self-repairing capability is good, and the anti-hydrolysis stability is high; the adhesive property and the state of the material can be adjusted by adjusting the proportion of the components and adjusting the adhesive property and the state of the material, so that the requirements on different adhesive properties in practical application are met, irregular wounds are filled, and rapid gelation is performed at the body temperature so as to adapt to the form of a surgical site; the effects of stopping bleeding, resisting inflammation, promoting revascularization and repairing nerves are also achieved.

Systems and methods for nerve repair can include a polymer configured to form an elongated conduit to receive an in vivo nerve therein and provide a mechanical stabilization to the in vivo nerve. The polymer can be configured to degrade upon being subjected to a dissolution solution to remove the mechanical stabilization from the in vivo nerve.

The application belongs to the field of medical biological materials, and relates to a preparation method of a hierarchical nanofiber composite film for nerve repair and application thereof. The method comprises the following steps: preparing multilayer nanofibers with different internal stresses through continuous multiple electrostatic spinning units, stacking the multilayer nanofibers with different internal stresses to form a composite nanofiber film with an internal stress gradient; chemically crosslinking the composite nanofiber film; performing adhesion molecule adhesion treatment on the upper surface of the composite nanofiber film; and preparing the hierarchical nanofiber composite film with an internal stress gradient and capable of self-deforming under the action of liquid to wrap nerves. The film provided by the application has excellent flexibility, biocompatibility and degradation performance, can efficiently and quickly adhere to the surface of damaged nerves, provides a good support microenvironment for nerve cell growth, promotes nerve regeneration and repair, and has a wide application prospect in the field of peripheral nerve injury repair.

Provided is a biosynthesis-based preparation method for a structural material of the human body. A polypeptide of the present application contains the structure of a C-terminal region of (repeating unit)n or (repeating unit)n-; the repeating unit contains an amino acid sequence as shown in SEQ ID NO. 1. Recombinant type V humanized collagen prepared in the present application has high activity in promoting cell adhesion, and does not cause an immune response when applied to the human body; moreover, the preparation method is novel, allows for large-scale production of the recombinant type V humanized collagen, and is widely used in the preparation of structural materials of the human body. The application field includes preparation of high-end medical devices, such as biological dressings, human body biomimetic materials, plastic-beauty materials, organoid culture, cardiovascular stents, coatings, tissue injection filling, ophthalmic materials, gynecological biomaterials, nerve repair and regeneration, liver tissue and vessel repair and regeneration, and 3D printing of artificial organ biomaterials.

The invention relates to the technical field of biomedical materials, in particular to a high-conductivity peripheral nerve socketed pipe and a preparation method thereof. The nerve socketed pipe is a hollow cylindrical socketed pipe made of a material containing poly (octylene-citrate-co-octanol) POCO, and a regeneration promoting material is loaded in the hollow cylindrical socketed pipe; the regeneration promoting material comprises fetal calf serum and a DMEM cell culture medium. The POCO has the advantages of conductive activity, nerve regeneration induction, free radical scavenging ability, oxidation resistance, deformation resistance and the like, and has good degradability and biocompatibility, so that the nerve socketed pipe is prepared from poly (octylene-citrate-co-octanol), and the growth of axons is accelerated through electrical stimulation. Furthermore, at least one conductive monomer of pyrrole, aniline, p-phenylenediamine, o-methoxyaniline, thiophene, 3-hexylthiophene, 3-thiopheneacetic acid and 3, 4-ethylenedioxythiophene is adopted for modifying the POCO hollow cylindrical socketed pipe through in-situ oxidative polymerization, and the performance of the POCO hollow cylindrical socketed pipe serving as the neural restoration material is further improved.

The invention relates to the technical field of curative effect prediction, in particular to a curative effect prediction method and system for treating spinal cord injury by stem cells, and the method comprises the following steps: obtaining a multi-parameter metabolism sequence and constructing an activation rhythm map, recognizing a neural restoration synchronization section, extracting a metabolism dominant path and evaluating electrical signal response, generating a para-position map layer and predicting a curative effect trend. According to the method, through introduction of cross-time-sequence metabolism and trend synchronous identification of electrophysiological indexes, dynamic mapping of stem cell activation rhythms is realized, neural axon morphological changes and cerebrospinal fluid metabolism parameters are linked, and distribution positions of key response points in a treatment cycle are determined; the method comprises the following steps: establishing a causal path based on a sequence of metabolic mutation and neural restoration events, extracting an evolution stage dominated by a metabolic driving effect, marking a matching trend of a curative effect response and an intervention path on a time axis, identifying the number and positions of trend convergence, withdrawal and interruption areas, and realizing collaborative prediction of a restoration progress and a curative effect trend.

The scheme provides a kind of engineered exosome double drug delivery system loaded with ergosterol and its preparation method and application, belongs to the field of nanomaterials and nanobiomedicine, based on the innovative ROS-responsive nanomaterial CHPG design, load the active ingredient ergosterol to play the role of treatment and repair, which realizes the blood-brain barrier penetration and precise drug release in lesion area through targeted functional modification;It is wrapped in the nasal mucosa-derived stem cell exosome to form a drug delivery system Exo@Erg-CHPG-M, which combines the natural biological characteristics of exosome and the precise targeting advantage of nanomicelle, significantly improves the drug delivery efficiency, in vitro and animal model verification show that, the system can improve the neurological impairment and brain tissue injury after ischemic stroke by synergistically regulating inflammation and neural repair pathways, the scheme provides a new technical scheme for the precise treatment of central nervous system diseases such as cerebral stroke.

The conduit carrier includes a carrier housing that includes first and second contact ends and a vacuum channel extending between them. The vacuum channel is configured to connect to a vacuum source via the first contact end. An engagement mechanism is connected to the second contact end. The engagement mechanism is configured to engage with the conduit in a releasable manner during nerve repair surgery. A separator is configured to extend from the second contact end. When the engagement mechanism engages with the conduit during nerve repair surgery, the vacuum channel is connected to a vacuum port in the conduit via the second contact end so that a vacuum source can draw a vacuum into the conduit, and the separator extends into the conduit through the vacuum port, creating a predetermined regeneration gap between the first and second nerve stumps inserted into the conduit.

The invention relates to the technical field of medical equipment, and discloses an adjustable nerve repair cannula and a preparation method, the adjustable nerve repair cannula is composed of a sheet fabric formed by interweaving warps and wefts, the tissue structure of the sheet fabric is a compact plain weave, the tissue structure is simple, and the weaving efficiency is higher; the linear density range of the warps and the wefts is 14tex-18tex, so that the light weight and the weaving density of the adjustable neural restoration cannula are kept, and the implantation burden of a patient is relieved on the basis of ensuring the restoration effect. Meanwhile, the microneedles are arranged at one end, in the width direction, of the flaky fabric, the flaky fabric can be directly wound and connected through the microneedles to form the tubular adjustable nerve repair cannula, the inner diameter range of the adjustable nerve repair cannula is changed by changing the connecting positions of the microneedles, and pressing-damaged nerves or broken-end nerves of different sizes can be directly wrapped conveniently; the application range of the adjustable neural restoration cannula is expanded, the manufacturing efficiency is improved, the process cost is reduced, and the growth of neurons is further facilitated.

The invention relates to a degradable nerve repair membrane and a preparation method thereof, and belongs to the technical field of nerve repair membranes, the degradable nerve repair membrane comprises a biological composite structure inner layer, a support outer layer and a bonding layer, and the biological composite structure inner layer and the support outer layer are bonded through the bonding layer. According to the invention, polypyrrole with excellent electrochemical activity, environmental stability, controllable conductivity and poor mechanical properties and collagen nanofibers with high biocompatibility and capable of forming a nano-scale fiber network are adopted to form a conductive-biological composite structure as an inner layer through electrostatic spinning, and the PLGA porous membrane is prepared through 3D printing. A PLGA porous membrane with parallel axial microchannels on the surface is used as an outer layer, so that the membrane body structure can effectively guide directional growth of axons while ensuring conductivity, accurate preparation can be realized according to needs, the degradation period is matched with the nerve regeneration rate, and the degradation time can be regulated and controlled through the proportion of a copolymer; the repairing effect is prevented from being influenced by too fast or too slow degradation.

The invention belongs to the technical field of biological materials, and particularly relates to a nerve repair scaffold and a preparation method thereof.The nerve repair scaffold is of a hollow double-shell fiber structure, the outer layer of the double-shell fiber structure is composed of PCL nanofiber filaments, the inner layer of the double-shell fiber structure comprises PCL-dECM fiber filaments, and the outer layer of the double-shell fiber structure is composed of PCL nanofiber filaments. The PCL-dECM cellosilk is a PCL cellosilk loaded with dECM, the PCL-dECM cellosilk of the inner layer is arranged in the axial direction of the double-shell fiber structure, and after the nerve repair stent is implanted, the nerve function recovery can be improved, and the biocompatibility is improved.

The invention relates to application of graphene-(RADA-IKVAV)-pranoprofen, eye drops and a preparation method of the eye drops. The invention relates to graphene-(RADA-IKVAV)-pranoprofen, the graphene-(RADA-IKVAV)-pranoprofen comprises graphene-polyacrylic acid, self-assembled oligopeptide RADA-IKVAV and pranoprofen, the self-assembled oligopeptide RADA-IKVAV is connected with the graphene-polyacrylic acid through a chemical bond, and the pranoprofen is loaded on the surface of the graphene-polyacrylic acid. The eye drops provided by the invention comprise graphene-(RADA-IKVAV)-pranoprofen, so that the drug loading capacity of pranoprofen in the eye drops is increased, the bioavailability of drugs is improved, and the eye drops are used for inhibiting eye inflammation; the coupled oligopeptide RADA-IKVAV simulates an extracellular environment, provides a suitable microenvironment for eyes, promotes nerve repair and regeneration by influencing nerve growth factors, and promotes optic nerve repair.

This invention relates to the field of biomedical materials technology, specifically disclosing a hydrogel material for spinal cord nerve repair containing magnetic nanospheres and its preparation method. The hydrogel material provided by this invention is a photocurable hydrogel formulated using chitosan, dimethylaminoethyl methacrylate, methacryloyloxyethyltrimethylammonium chloride, and magnetic iron oxide nanospheres. Compared with traditional spinal cord nerve repair materials, the hydrogel material provided by this invention has good biocompatibility, is safe and non-toxic, and has a simple and easy preparation method. Photocuring is performed when the hydrogel is injected into the spinal cord injury site, while a magnetic field is simultaneously applied to the injury site. This causes the magnetic nanospheres to rotate according to the magnetic field, forming a series of magnetic channels. As the hydrogel gradually solidifies, the magnetic channels are permanently retained. During the spinal cord nerve repair process, the magnetic channels guide nerve growth, preventing incorrect nerve connections and ensuring the effectiveness of nerve repair.

The invention provides a sequential slow-release hydrogen sulfide nerve repair conduit and a preparation method thereof, and belongs to the technical field of biological materials. The sequential slow-release hydrogen sulfide nerve repair conduit comprises degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel and a pH response hydrogen sulfide donor, wherein the degradable polymer conduit is filled with the degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, and the pH response hydrogen sulfide donor is loaded on the hydrogel. In the application, the pH response donor can respond to an acidic microenvironment of degradation of polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, release hydrogen sulfide and relieve the acidic environment generated by degradation of the hydrogel at the same time, and form a dual supply mechanism with cysteine (endogenous hydrogen sulfide precursor) obtained by degradation of the hydrogel; nerve regeneration and angiogenesis are synergistically promoted, and oxidative stress is regulated; and the hydrogel is accelerated to degrade during excessive oxidative stress, so that hydrogen sulfide is released as required, and the core problems of insufficient blood supply, unbalanced acidic microenvironment and slow nerve regeneration are synchronously solved.

The present invention discloses a walnut oligopeptide powder with the functions of promoting peripheral nerve regeneration and repairing peripheral nerve injuries and a preparation method thereof, belonging to the technical field of walnut oligopeptide production. The walnut oligopeptide powder uses walnut cake as a raw material, is enzymatically hydrolyzed by an enzyme preparation, and is prepared by fermentation with Kluyveromyces marxianus and Lactobacillus helveticus. The enzyme preparation is trypsin and ficin. Compared with the prior art, the yield of the walnut oligopeptide powder of the present invention is higher. Moreover, it has been proven by experiments that the walnut oligopeptide powder prepared by the present invention can effectively extend the length of peripheral motor nerves and has a good effect on nerve repair.

The invention discloses a method for promoting vascularization of an allogeneic nerve repair material, and belongs to the field of peripheral nerve repair materials. The vascularization-promoting allogeneic nerve repair material is based on an acellular allogeneic nerve, and an astragalus polysaccharide alginic acid sustained-release microcapsule is compounded on the surface of the acellular allogeneic nerve. The decellularized allogeneic nerve is degreased and decellularized in a physical mode, and antigen components are effectively removed; the astragalus polysaccharide is a natural extract from tonifying traditional Chinese medicine astragalus, and compared with an angiogenesis factor, the astragalus polysaccharide has the advantages of no tumorigenicity, stable activity, low price and the like. The vascularization-promoting allogeneic nerve repair material disclosed by the invention can solve the problem of insufficient cell and nutrition supply caused by slow vascularization after allogeneic nerve implantation, and promotes nerve repair.

The invention discloses a preparation method of an amniotic membrane loaded with procyanidine and mecobalamine, which comprises the following steps: soaking an amniotic membrane in a solution containing procyanidine, mecobalamine and N-hydroxysuccinimide, and reacting to obtain the amniotic membrane loaded with procyanidine and mecobalamine. The amniotic membrane is selectively loaded with procyanidine and mecobalamin, has the functions of resisting inflammation and bacteria and promoting corneal epithelium and nerve repair, and solves the problem that the repair promoting function of the treated amniotic membrane is reduced.