82 results about "Nerve axon" patented technology

A flat interface nerve electrode is provided along with a method for its use. The electrode provides a plurality of conductive elements embedded in a non-conductive cuff structure, which acts to gently and non-evasively redefine the geometry of a nerve through the application of a force so as to apply pressure to a nerve in a defined range, namely from 2 to 40 mmHG and more preferably from 15 to 30 mmHG and most preferably from 15 mmHG to 20 MMHG. This range is selected to minimize the reduction of blood flow within the tissue, which preferably is at least 70% of the initial value, more preferably 90% of the initial value. The cuff has an opening, which is elongated relative to the diameter of the nerve to which it is applied. Preferably, the cuff is constructed from an elastic bio-compatible material having top and bottom beam members configured to define a nerve opening. The cuff is open at one side and has a connection at the other side which results in a spring force being applied through the surfaces of the nerve opening to the subject nerve. During implantation the open sides of the cuff are closed so as to capture the nerve in the cuff. As the nerve is reshaped, specific nerve axons become more easily addressed through the epineurium by the embedded conductive elements.

The invention relates to polypyrrole biological conductive hydrogel, as well as a preparation method and application thereof. The preparation method comprises the following steps; preparing carboxymethylchitosan hydrogel from carboxymethyl chitosan by using a crosslinking agent under a certain condition, and performing freeze-drying treatment; performing electrochemical synthesis on a pyrrole monomer to obtain polypyrrole, adding a dopant into the polypyrrole to perform modification and performing ball-milling and crushing to enable the particle size to be matched with the pore diameter of pores of the carboxymethylchitosan hydrogel; blending nerve growth factors into water, adding modified polypyrrole particles, stirring and dispersing uniformly, putting the carboxymethylchitosan hydrogel to enable the solution to be completely absorbed, adding water, vibrating, and completely swelling and balancing to obtain the polypyrrole biological conductive hydrogel. When the polypyrrole biological conductive hydrogel is applied to neural restoration, sustained release of neurotrophic factors can be completed, nerve cells can be subjected to certain electrical stimulation through bioelectrical or external electrical stimulation, proliferation and differentiation of the nerve cells are promoted through double effects of electric stimulation and neurotrophic factor induction, and nerve axon growth is guided.

The invention relates to a conductive parallel fiber membrane which is capable of promoting rapid repair of peripheral nervous tissues and is connected to an extracellular matrix, wherein the thin film (the fiber membrane) can serve as a biofunctional nerve scaffold. The conductive parallel fiber membrane which is connected to the extracellular matrix and is of a core-shell structure is prepared by virtue of a three-step method, namely preparing parallel fibers through electrostatic spinning, covering conductive polypyrrole through electrochemical oxidation, and conducting human skin fibroblast culture and splitting cells by virtue of a triton x-100 / ammonia water mixed solution. According to the invention, the composite fiber membrane, by virtue of the parallel structure, can guide the growth of nerve fibers in a directional mode, by virtue of the extracellular matrix, adhesion of the nerve cells and axon thereof is improved, and by virtue of the conductive polymer (the conductive polypyrrole), the growth of the nerve axon is promoted through electric stimulation; and with a synergistic effect among the three means, the regeneration and repair of nerves are promoted. A preparation device adopted by the invention is simple and easy, simple to operate, wide in material source and low in cost; and the prepared nerve scaffold is good in biocompatibility and functionality, and the nerve scaffold is expected to be applied to clinical field in the future.

The invention relates to an implanting type degradable device for promoting nerve regeneration after ambient nerve implanting. The implanting type degradable device comprises a hollow cylindrical device which is formed by jointing two symmetric semi-cylindrical subassemblies, wherein the cylindrical device is internally divided into a plurality of independent small chambers for loading nerve regeneration promoting materials with different concentrations; the two ends of the cylindrical device are opened, the ambient nerve for implanting repairing material can pass through one end of the cylindrical device, and pass out through the other end of the cylindrical device; a clearance between the two semi-cylindrical subassemblies can be sealed to form a closed tubular structure through medical bioprotein; and the semi-cylindrical subassemblies are manufactured by degradable biocompatibility materials. The implanting type degradable device has the characteristics of simplicity, convenience, safety and high efficiency; the implanting type degradable device can be used for loading various nerve regeneration promoting materials, and driving the nerve regeneration promoting materials to move around the ambient nerve implants by forming a concentration gradient based on the remote end and the near end of the nerve, so that a microenvironment beneficial to the regeneration of the ambient nerve axon can be formed.

The invention discloses a method for preparing an artificial nerve conduit based on 3D printing and the artificial nerve conduit. The problem that clinical application is limited by autogenous nerve grafting is solved. A silk fibroin solution is prepared from fibroin; a silk fibroin microsphere suspension is prepared; growth factors are loaded in silk fibroin microspheres; silk biological ink containing nerve cells is prepared; the silk fibroin microspheres loaded with the growth factors and PLGA are used for preparing a composite; the silk biological ink is filled in a first printing nozzle of a 3D printer, and the composite is filled in a second printing nozzle; the second printing nozzle is controlled to print an outer tube of the artificial nerve conduit, then the first printing nozzle is controlled to print a plurality of inner tubes of the artificial nerve conduit, and finally cell culture differentiation is conducted to form the nerve conduit. The nerve conduit prepared through three-dimensional biological printing provides a base body for adhesion growth of nerve cells, provides guidance for nerve axon combination, can promote nerve regeneration and replaces autogenous nerve grafting.

The invention relates to a bionic nerve graft used for peripheral nervous recovery and a preparation method and application thereof. The bionic nerve graft comprises a chitosan film conduit and a fibrous protein hydrogel beam positioned in the chitosan film conduit, wherein the fibrous protein hydrogel beam is prepared from fibrous protein through an electrospinning technique. The bionic nerve graft prepared by the preparation method is formed by the filling of the chitosan film conduit and the fibrous protein hydrogel beam of a multi-stage orientation structure, wherein a nerve regrowth space is effectively supported by a chitosan film, the orientational regeneration of a nerve axon is effectively guided by the fibrous protein hydrogel beam of multi-stage orientation, the cell affinity and the mechanical property of whole materials are good, and in addition, degrading speed and the repairing speed of peripheral nervous tissues are matched.

Provided are a clinically applicable drug for radical cure of Alzheimer's disease (AD), and a drug for treating neurological diseases associated with axonal dysfunction other than AD, wherein the drug utilizes the mechanism of action of the AD radical cure therapy. The drugs are oral drugs, each comprising one or more compounds selected from diosgenin, a diosgenin derivative and a pharmaceutically acceptable salt thereof, the one or more compounds being suspended in an edible oil.

The invention provides a cylinder support for repairing spinal cord injury. The cylinder support comprises a collagen sponge cylinder support body. An epidermal growth factor receptor antibody cetuximab is crosslinked with the collagen sponge cylinder support body. Neural stem cells are loaded through the collagen sponge cylinder support body crosslinked with the epidermal growth factor receptor antibody cetuximab so as to promote transplanted neural stem cells to be differentiated into neurons, and the cylinder support which facilitates injured spinal nerve regeneration and functional reconstruction is constructed. The collagen sponge cylinder support body has ultrahigh porosity and provides sufficient space for cell growth. More importantly, controlled-release of the antibody cetuximab can relieve effect myelin protein in a microenvironment after spinal cord injury, promotes transplanted neural stem cells to be differentiated into neurons, restrains transplanted neural stem cells from being differentiated into astroglia cells, and also promotes growth of nerve axons so as to facilitate injured spinal nerve regeneration and functional reconstruction. In addition, the invention further provides an application method of the cylinder support for repairing spinal cord injury.

The invention relates to a two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and a preparing method thereof. Firstly, a parallel porous polylactic acid filament membrane with the diameter ranging from 50 nm to 2000 nm is put into a mixed aqueous solution containing sodium alkyl sulfonate, polyglutamic acid, FeCl3 and pyrrole, then a sufficient reaction is carried out for 2-24 h with an in-situ polymerization method, the surface of the parallel filament membrane is evenly coated with polypyrrole nano-particles, washing and drying are carried out, and then conductive polypyrrole wrapped polylactic acid fiber filament membrane with the diameter ranging from 60 nm to 2500 nm is obtained; then, protein of the two nerve factors, namely, NGF and NT-3, is connected to the parallel conductive porous composite filament membrane in sequence with the carbodiimide technology, and the conducive composite filament membrane which can release nerve factors and promote nerve axon elongation and peripheral nerve repair under electrical field stimulation is obtained. The two-nerve-factor NGF and NT-3 connected polypyrrole-polylactic acid parallel conductive porous composite filament membrane and the preparing method thereof have the advantages that implementation is easy, the nerve factor connection efficiency is high, the material conductivity is high, and the nerve repair time is short.

An object of the present invention is to provide a non-peptidic nerve axon and / or neurite outgrowth agent for allowing a nerve axon and a neurite to elongate. 3-(Aminocarbonyl)-1-[5-O-[[1-(6-amino-9H-purin-9-yl)-1-deoxy-β-D-ribofuranos-5-O-yl]phosphonyloxy(oxylato)phosphinyl]-β-L-ribofuranosyl]pyridinium that is an analogue of nicotinamide adenine dinucleotide (NAD) comprising β-L-ribose as the sugar component is used as a nerve axon and / or neurite outgrowth agent or composition, a cancer cell growth suppressing and / or apoptosis inducing agent, or a cancer cell growth suppressing and / or apoptosis inducing composition.

The invention provides a neural restoration sleeve and a preparing method and application thereof. The neural restoration sleeve is a double-layer fiber sleeve, the inner layer of the sleeve is an orientation polymer fiber layer, the outer layer of the sleeve is a non-orientation polymer fiber layer, bioresorbable polymers are adopted, and the nerve growth promoting factors are embedded in the inner layer. The neural restoration sleeve prepared through the preparing method can induce nerve cells to grow in the fiber orientation, meanwhile, the nerve growth promoting factors are embedded in the inner layer to promote growth of nerve axon and accelerate nerve tissue restoration, the non-woven non-orientation polymer fiber layer on the outer layer plays a role in supporting and endows the neural restoration sleeve with high strength and stability, and the polymers on the inner layer and the outer layer are the bioresorbable polymers, have high biocompatibility, will not cause chronic inflammation reaction and are high in safety; the preparing method is simple, and industrial production can be realized.

The invention relates to an aquagel material used to recover hurt nerve center. Wherein, it uses nerve center cross method to graft the layer adhesive protein (Laninin) with nerve grow activity to the molecule skeleton of transparent acid aquagel, to rpare the aquagel frame, which can accelerate the regeneration of nerve axon.

The invention discloses a nerve axon stretching grower which consists of a culturing and stretching control system and a mechanical device, wherein the culturing and stretching control system comprises a cell culture box, an upper computer, a controller and a stepping motor; the mechanical device comprises a coupler which is connected with the stepping motor, a ball screw linear sliding table, a stretching connecting block, a cell stretching grower, a device supporting rack and a chassis; the controller is connected with and drives the stepping motor to rotate so as to drive the ball screw linear sliding table at one end of the coupler to generate displacement, and the cell stretching grower is fixedly arranged on the device supporting rack and is used for indirectly stretching nerve axons by virtue of the stretching connecting block which is fixedly arranged on the linear sliding table. Different input commands can be set by virtue of the controller so as to regulate the growing direction and speed of the nerve axons. In addition, electrode contacts can be plated on a stretching film and a bottom film and can be connected with a multichannel neural signal recording and stimulating system for recording neural signals of the nerve axons and selectively stimulating different sites.

The invention relates to a PLGA three-dimensional nerve conduit and a preparation method thereof. The PLGA three-dimensional nerve conduit comprises a laminin coating and an orientation yarn core layer. The preparation method comprises the steps that a polylactide acid-glycolic acid copolymer (PLGA) is dissolved into a solvent, and the materials are mixed to be uniform to obtain a spinning solution; single-spray-head electrostatic spinning is conducted to obtain the PLGA three-dimensional nerve conduit containing the orientation yarn core layer, the PLGA three-dimensional nerve conduit is bonded with laminin through covalent bonds, and then the PLGA three-dimensional nerve conduit which is provided with the laminin coating and contains the orientation yarn core layer is obtained. The three-dimensional nerve conduit which is provided with the laminin coating and filled with orientation yarn has the good mechanical property, biological activity and degradation property; protein in the coating can promote nerve cell adhesion and nerve axon regeneration, and meanwhile, the yarn in the core layer can induce nerve cells to grow in a crawling mode in the yarn orientation direction, so that regeneration and reconstruction of three-dimensional nerve tissue are promoted, and important application can be achieved in peripheral nerve tissue repair and regeneration.

The invention discloses a heterologous decellularized nerve graft with a tissue adhesion preventing function and a preparation method thereof. The heterologous decellularized nerve graft capable of preventing tissue adhesion consists of a heterologous decellularized nerve graft and an anti-adhesion macromolecular layer attached to the surface of the heterologous decellularized nerve graft; the heterologous decellularized nerve graft is an extracellular matrix which is obtained through subjecting in-vitro peripheral nerves of a healthy mammal to decellularizing. The anti-adhesion heterologous decellularized nerve graft disclosed by the invention is wide in source, low in price, good in biocompatibility and safe in use and meanwhile is free of immunogenicity; a microstructure of the extracellular matrix of the peripheral nerves is completely reserved, and thus, a natural piping support and a nutrition environment are provided for regenerating nerve axons and restoring a nerve conduction function; the anti-adhesion macromolecular layer of the heterologous decellularized nerve graft capable of preventing tissue adhesion, disclosed by the invention, can be changed into gel coats which are attached to surfaces of the decellularized nerves after the heterologous decellularized nerve graft is grafted in vivo, so that the action of preventing heterologous decellularized nerves from being adhered to periphery tissue can be excellently exerted.

A neural interfacing device is disclosed. The neural interfacing device may include at least one microneedle electrode. The microneedle electrode may have one or more microneedles. The one or more microneedles may be shaped and positioned such that when the neural interfacing device is applied to a nerve, the one or more microneedles penetrate a nerve epineurium without any portion of the microneedle electrode penetrating any nerve axon beyond a depth of 500 micrometers.

The invention discloses a purpose of curcumin or a drug-loading system thereof in preparation of the drug for treating penis erection dysfunction. An experiment proves that the curcumin and a curcuminslow release drug film can up-regulate the expression of GAP-43 and MAP-2 to accelerate growth of the nerve axon and can up-regulate the expression of Oct-6 and Krox-20 to accelerate Schwann cells tosynthesize and secrete myelin sheath-associated protein so as to accelerate formation of the nerve axon myelin sheath. Meanwhile, repair and regeneration after a rat suffers from cavernous body nerveinjury, neural pathway continuity of the cavernous body is recovered, and expression of penis tissue nerve terminal nNOS mRNA and protein is increased. The penis erection function of the rat who suffers from the cavernous body nerve injury can be further improved, meanwhile, a penis tissue fibrosis degree is lowered, and rehabilitation of the penis after the cavernous body nerve injury is effectively accelerated. Therefore, the curcumin and the drug-loading system thereof perform a good application prospect in the preparation of the drug for treating the penis erection dysfunction and repairing the cavernous body nerve injury.

The invention belongs to the field of biomedical engineering, and relates to a tissue engineered artificial optic nerve conduit and a preparation method thereof. The tissue engineered artificial optic nerve conduit comprises a hollow conduit woven from polylactic-polyglycolic acid filaments (PLGA), polyglycolic acid (PGA) filaments and transgenic schwann cell suspension, wherein chitosan polymer is coated on the surface of the conduit; an external bracket of artificial optic nerve is constructed by the PLGA hollow conduit; and the PGA filaments are filled in an internal bracket in which the artificial optic nerve is constructed. The conduit can effectively promote regeneration of damaged optic nerve axon, and has the advantages of persistently providing nerve nutritional support, guiding the regenerated nerve axon and the like; the conduit has high tissue compatibility and safety; the specifications of the transfected gene and the conduit can be adjusted according to requirements; and the conduit and the method can be used for experimental research of optic nerve regeneration and expected to promote regeneration and restoration of damaged optic nerve in clinical use.

The invention relates to a composite stem cell bionic nerve conduit. The composite stem cell bionic nerve conduit comprises a braided hose side wall; a round hose is formed via coiling of the braided hose side wall; and gel and stem cells are arranged in the round hose formed via coiling of the braided hose side wall. The materials of the composite stem cell bionic nerve conduit are high-molecular degradable materials, are easily available, and possesses certain strength and excellent biocompatibility; the nerve conduit side wall formed via braiding possesses a lot of pores with the same directivity; the compactness of the nerve conduit side wall can be adjusted via different braiding methods, so that it is beneficial for nutrient delivery and exchanging; atmospheric pressure plasma jet processing is capable of increasing nerve conduit braided fiber surface area, and improving conduit hydrophilic performance and cellular affinity; the gel in the nerve conduit is semi-solidified, a support interface and a material exchange interface are formed by the semi-solidified gel, so that it is beneficial for uniform distribution of stem cell in the nerve conduit, and cell fixing and differentiation, and it is also beneficial for growth of regenerated nerve axon and formation of myelin sheath, and repairing of long nerve.

The invention relates to a preparation method and an application of a carboxymethyl chitosan composite nerve conduit which has high tensile strength and can be stitched and fixed. The preparation method comprises the following steps of: blending the crosslinking carboxymethyl chitosan solution and polyving akohol solution at different volume ratios; injecting the crosslinking fluid into a mould; performing repeated freeze thawing, and performing air drying and curing; washing with water for injection; removing the solvent; and performing air drying. According to the electrophysiological and histological observation results of a peripheral nerve defect animal model, the prepared nerve conduit can realize bridge connection of the nerve-defect broken ends, has high tensile strength and long in-vivo hold time, and can promote nerve regeneration; and the nerve axon can grow along the conduit. The preparation method provided by the invention has the characteristics of simple technology and low cost, can replace the traditional nerve sewing method, and has a relatively good application prospect in clinical treatment of peripheral nerve defect.

The invention relates to NGF (Nerve Growth Factor) injectable in-situ hydrogel as well as preparation and application thereof. The NGF injectable in-situ hydrogel is obtained by adding EDC(1-ethyl amide crosslinking agent-3-(3-dimethyl amino propyl) carbide imine) and NHS (N-hydroxysuccinimide) into an aqueous solution system of hyaluronic acid and chitosan hydrochloride, and adding an NGF after HA-NHS-active ester intermediate by the hyaluronic acid under the action of the EDC / NHS under an acidic condition. The hydrogel has a pH sensitive feature; the conversion process of solution-gel can be completed under physiological conditions of a human body; the NGF injectable in-situ hydrogel can be subjected to in-situ curing under the action of pH in an acceptor at a nerve damage part after the NGF injectable in-situ hydrogel is injected into nerve tissue; the traumatic property of a surgery is avoided. The NGF can be released slowly in vitro by utilizing the environmentally sensitive feature of the hydrogel; the problems of low activity, burst release and the like caused by the facts that a half-life period is short and the diffusion and the degradation are too quick of the NGF are effectively solved; high bioactivity of the NGF is kept; the extension and the myelination of a nerve axon are promoted; therefore, the purpose of repairing nerves is achieved.

The invention relates to antigen of neural recognition molecule contactin 6, and an amino acid sequence of the antigen is shown in SEQ ID No.1. The invention also discloses an antibody prepared by the above antigen of neural recognition molecule contactin 6. The invention further discloses an application of the antibody in preparation of drugs for treating spinal cord injury. The antibody of the invention can effectively block the homologous association of contactin 6 and promote nerve axon growth, and the application of the antibody used in spinal cord injury can promote nerve axon regeneration and functional improvement.

The invention provides in-situ injectable hydrogel for treating central nervous injury and a preparation method of the in-situ injectable hydrogel. The hydrogel is formed by performing click chemical coupling on X-Y through functional groups on arginine-glycine-aspartic acid modified multi-arm polyethylene glycol-X and multi-arm polyethylene glycol-Y, nano / micron particles and growth factors of slow-release immunoregulation and / or antioxidant drugs loaded on the hydrogel through chemical coupling reaction are coated in the hydrogel. The injection hydrogel disclosed by the invention can be used for precisely and slowly releasing the immunomodulatory and / or antioxidant drug and the growth factor locally at an injured part, so that the formation of a cystic cavity after spinal cord injury is prevented, secondary injury caused by neuroinflammation is relieved, residual spinal cord nervous tissues and axons are protected, and the formation of glial scar tissues is reduced; a penetrable extracellular matrix environment is provided for regeneration of body nerve axons, further recovery of electrophysiology and motor functions is promoted, and the method can be used for repairing injury of soft tissues such as spinal cords and the like.

The invention discloses a preparation method and application of a bionic silicified collagen material. The preparation method disclosed by the invention comprises the step of soaking ACE collagen in a pretreatment solution to obtain a bionic silicified collagen material, wherein the pretreatment solution is composed of choline chloride and an orthosilicic acid solution. The bionic silicified collagen material constructed by the invention has good physical and chemical properties, and can promote the growth of peripheral sensory nerve axons and activate a sensory nerve mTOR signal channel to secrete Semaphorins 3A.

An axon regeneration promoter which contains an RGM-like protein inhibitor as the active ingredient. Examples of the RGM-like protein inhibitor include anti-RGM-like protein antibodies, RGM-like protein inhibitors such as Y27632 and antisense nucleic acids and double-stranded DNAs capable of inhibiting the transcription or translation of RGM-like proteins. This axon regeneration promoter is efficacious in the regeneration of a central nerve axon and thus contributes to, for example, the treatment of a patient suffering from injury in the central nerve system such as the spinal cord by a traffic accident, cerebrovascular disorder, etc.

The invention provides a silk protein nerve conduit. The silk protein nerve conduit comprises a conduit part and filling sponge filled in the conduit part, wherein the conduit part comprises a conduitbase body and a silk protein film compounded on the surface of the conduit base body; the conduit base body is formed by weaving silk protein fibers; and the filling sponge is formed by silk proteinnanofiber gel with an orientation structure. Compared with the prior art, the silk protein nerve conduit has the advantages that the silk protein nanofiber sponge with the orientation structure is arranged in the conduit, and the orientation structure is consistent with the direction of nerve axon, so that an orientation induction signal can be provided, migration and proliferation of nerve cellsare promoted, regeneration and extension of nerve fibers can be guided, and the function of the nerve conduit is remarkably improved; and therefore, the silk protein nerve conduit is combined with thesilk protein orientation sponge, so that the problems of mechanical property and orientation induction are solved.

The invention provides a preparation method of a double-layer nerve conduit. Due to the design of a double-layer structure, the unification of mechanical property requirements and biological functionrequirements on the nerve conduit is achieved; acellular matrixes uniformly distributed on an inner layer can endow each fiber with biological functionality, and an oriented fiber structure can guideoriented rapid growth of nerve axons and oriented rapid migration of Schwann cells, so rapid and accurate repair of nerves is promoted; and a compact porous structure on the outer layer not only allows sufficient exchange of substances, but also can play a barrier role and prevent formation of scars. Since the double-layer nerve conduit has high mechanical properties, the double-layer nerve conduit can meet the requirement for the suturing property of a graft in the in-vivo transplantation process, and provides necessary support for nerve regeneration space.