23 results about "Nerve axon" patented technology

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The invention relates to the technical field of curative effect prediction, in particular to a curative effect prediction method and system for treating spinal cord injury by stem cells, and the method comprises the following steps: obtaining a multi-parameter metabolism sequence and constructing an activation rhythm map, recognizing a neural restoration synchronization section, extracting a metabolism dominant path and evaluating electrical signal response, generating a para-position map layer and predicting a curative effect trend. According to the method, through introduction of cross-time-sequence metabolism and trend synchronous identification of electrophysiological indexes, dynamic mapping of stem cell activation rhythms is realized, neural axon morphological changes and cerebrospinal fluid metabolism parameters are linked, and distribution positions of key response points in a treatment cycle are determined; the method comprises the following steps: establishing a causal path based on a sequence of metabolic mutation and neural restoration events, extracting an evolution stage dominated by a metabolic driving effect, marking a matching trend of a curative effect response and an intervention path on a time axis, identifying the number and positions of trend convergence, withdrawal and interruption areas, and realizing collaborative prediction of a restoration progress and a curative effect trend.

A method of interrupting sympathetic stimulation to the cardiovascular system of a patient in need thereof includes navigating a probe of a hand-held cryogenic therapy apparatus to a stellate ganglion or an autonomic tissue area peripheral to the stellate ganglion of the patient, the probe including a needle configured to produce a cooling zone for focused cryogenic therapy, aligning the needle with one or more desired nerves of the stellate ganglion or the autonomic tissue area peripheral to the stellate ganglion, and producing the cooling zone to provide cryogenic therapy to the desired nerves of the stellate ganglion or the autonomic tissue area peripheral to the stellate ganglion at a temperature sufficient to cause axonotmesis of the nerves.

The invention relates to the technical field of medical apparatus and instruments, and discloses a nerve repair fixing cannula, a flexible connection sheet can be bent into a tubular shape, so that two ends of the flexible connection sheet respectively wrap the peripheries of two nerve broken ends, the inner side of the flexible connection sheet is provided with a growth space, a tubular structure for nerve bridging is established, nerve axons grow directionally, and the two nerve broken ends are kept aligned; the multiple microneedles are arranged at the two ends of the flexible connecting piece at intervals, the connecting tension of the nerve bundle layer and the flexible connecting piece is dispersed, and the pain feeling of a patient is relieved; a needle head of the microneedle is used for being inserted into an adventitia so as to fix the bent flexible connecting piece to the nerve broken end, a needle rod penetrates through the flexible connecting piece, and the needle rod and the outer tangent plane of the bent flexible connecting piece at the needle threading position are obliquely arranged at a first preset angle, so that the mechanical stability of the nerve repair fixing sleeve installed on the nerve broken end is improved; the nerve broken end can be quickly and conveniently fixed, meanwhile, the damage to nerve tracts is reduced, and the situation that the nerve function recovery is affected by scars is avoided.

The present invention discloses a bioactive scaffold for slowly releasing Apt19S to recruit endogenous stem cells and guide the straight regeneration of central nervous system axons and its application. The bioactive scaffold is composed of acellular optic nerve (DON) and aptamer Apt19S, and its preparation method comprises the following steps: 1. preparing a DON scaffold. 2. activating the carboxyl group on the surface of the DON scaffold. 3. The DON scaffold is combined with amino-modified Apt19S through an amidation reaction to obtain the DON‑Apt19S scaffold (abbreviated as DON‑A). This bioactive scaffold can continuously release Apt19S, recruit ALPL-positive endogenous neural stem cells and mesenchymal cells, guide the straight regeneration of central nervous system axons, and provide a microenvironment for neurogenesis and angiogenesis for in situ spinal cord repair. The bioactive scaffold has important transformation prospects and application value in the repair of central nervous system injuries.

The invention provides application of Kcnh4 in preparation of a medicine for treating nerve axon injury related diseases, relates to the technical field of biomedicine, and is technically characterized in that the application provides application of Kcnh4 in preparation of a medicine for treating nerve axon injury related diseases. According to the application, the expression change of Kcnh4 in dorsal root ganglion (DRG) tissues at different time points after sciatic nerve injury of the SD rat is firstly detected; a verification experiment proves that in-vitro interference of Kcnh4 can significantly promote growth of DRG and cortical neuron axons, and shows that Kcnh4 is an important molecular target for nerve injury repair.

The invention provides an animal body electrode myelination electric control system and method, and the system comprises an electrode mechanism which comprises an electrode substrate, and a first electrode, a second electrode, a third electrode, a fourth electrode, a fifth electrode and a sixth electrode which are disposed on the electrode substrate. The electrode mechanism, the resistor and the waveform function generator are sequentially connected in series to form an electric control system. When the electrode mechanism is used, the electrode mechanism is implanted into a to-be-operated object and then fixed on soft tissue of an injured area through an absorbable suture, and the two ends of a series electric control circuit are connected with an external power supply to form an electric control system. The first electrode, the second electrode, the third electrode, the fourth electrode, the fifth electrode and the sixth electrode are externally connected with copper wires for electrode stimulation selection, multi-path and multi-direction electrical stimulation scheme adjustment is achieved, growth of nerve axons can be promoted, remyelination of the axons can be promoted, recovery of nerve functions is accelerated, and popularization and application are facilitated.

The present invention discloses a brain-computer interface based on nerve tentacles, comprising a plurality of nerve tentacles and an electronic interface; the first end of the nerve tentacles is used for two-way electrical signal interactive communication; the second end of the nerve tentacles is connected to the first end of the electronic interface, and the second end of the electronic interface is used to connect to an external device; the electronic interface comprises a plurality of axon electrodes, and the axon electrodes are correspondingly arranged at the second end of the nerve tentacles; nerve axons are induced to grow in the branches of the nerve tentacles; the axon electrodes comprise insulating microtubes, microelectrodes and wires; the tail end of the nerve axon passes through the insulating microtube and bends and extends toward the head end of the nerve axon; the microelectrode is arranged in the insulating microtube; one end of the wire is connected to the microelectrode, and the other end is used to connect to an external device; the present invention avoids intracranial immune rejection reaction and improves the service life of the interface by growing nerve axons in the branches of the nerve tentacles, and the nerve axons serve as the electrical recording or electrical stimulation of the brain.

The invention discloses a programmed controllable slow-release bionic spinal cord graft and a preparation method and application thereof, and belongs to the field of medical biomaterials.The preparation method comprises the steps that a micro-nano oriented fiber scaffold with a groove is synthesized on the basis of recombinant NT3, silk fibroin, bovine collagen and a neurotrophic factor NGF; the programmed controllable slow-release bionic spinal cord graft is jointly constructed by combining an acellular matrix and an exosome derived from human umbilical cord mesenchymal stem cells with a programmed slow-release system of GelMA drug-loaded microspheres containing cyclosporine a; multiple technologies such as bionic tissue engineering, a micro-fluidic technology, a drug-loaded microsphere controllable slow-release technology, an acellular matrix and exosome are combined, spinal cord injury repair is facilitated, good biocompatibility is achieved, nerve axons are promoted to grow in an oriented mode, immune regulation can be given in the early stage of spinal cord injury, the nerve regeneration microenvironment after spinal cord injury is improved, and the nerve regeneration effect is improved. The method is beneficial to nerve regeneration and functional reconstruction after spinal cord injury.

The invention belongs to the technical field of biology, and particularly relates to a corneal nerve bionic guiding support based on an electrospinning technology. The corneal nerve bionic guide stent provided by the invention is high in porosity and beneficial to cell attachment, migration and neurite extension, and during use, the stent structure can simulate the arrangement direction of corneal nerves, can effectively guide nerve axons to grow in a specific direction, and improves the nerve regeneration efficiency; according to the invention, the silk fibroin is compositely modified by adopting a plurality of growth factors such as NGF, NT-3, NT-4 / 5, NPY and VEGF, so that a multi-factor synergistic effect is realized, and related signal channels of nerve regeneration can be more comprehensively activated. After the polycaprolactone and the silk fibroin are compounded, complementary advantages are formed, the structural strength requirement of the cornea tissue engineering scaffold is met, good flexibility is kept, and the scaffold has good cell affinity and tissue integration capacity and is suitable for tissue engineering scaffolds.

The multi-electrode cell myelination electric control system comprises a multi-electrode plate, a resistor and a waveform function generator, the multi-electrode plate, the resistor and the waveform function generator are sequentially connected in series to form a series electric control circuit, and the two ends of the series electric control circuit are connected with an external power source to form the electric control system. The multi-electrode plate comprises a flexible substrate and electrodes, a first electrode area and a second electrode area are arranged on the flexible substrate, and six electrodes are arranged in each of the first electrode area and the second electrode area. The multi-electrode plate, the resistor and the waveform function generator are sequentially connected in series to form a series electric control circuit, the two ends of the series electric control circuit are connected with an external power supply to form an electric control system, multi-path and multi-direction electric stimulation scheme adjustment is achieved by controlling the electrodes on the two sides to discharge, growth of nerve axons can be promoted, and the nerve stimulation effect is improved. And remyelination of axons can be promoted, so that recovery of neurological functions is accelerated, and popularization and application are facilitated.

The present invention belongs to the technical field of preparation of anti-tumor drugs, and more specifically, relates to a drug-loaded bacterial outer membrane vesicle that targets and intervenes in the tumor neural microenvironment and a preparation method thereof. The drug-loaded bacterial outer membrane vesicle includes a bacterial outer membrane vesicle, and a small molecule drug with a neuroinhibitory effect wrapped by the bacterial outer membrane vesicle, and the surface of the bacterial outer membrane vesicle is also modified with a nerve cell targeting molecule. The drug-loaded bacterial outer membrane vesicle provided by the present invention can effectively target nerves in the tumor microenvironment, while effectively blocking the interaction between nerves and tumors, inhibiting nerve infiltration and nerve axon growth in the tumor microenvironment, and thus inhibiting tumor growth. The preparation method of the present invention is simple and highly operable, and the prepared drug-loaded bacterial outer membrane vesicle has good biocompatibility and good clinical application prospects.

The invention relates to the technical field of microbial medicines, and particularly discloses application of intestinal golden thread bacteria in preparation of a medicine for preventing or treating peripheral nerve injury. A mouse model proves that the intestinal golden thread bacteria can regulate the injury microenvironment in the peripheral nerve injury repair process, guide nerve axons to extend in the expected direction and reduce uncontrolled axon growth and wrong dominance, so that the structural accuracy of nerve regeneration is improved.

This invention discloses an anisotropic piezoelectric thin film, its preparation method, and its applications, belonging to the field of biomedical materials technology. The anisotropic piezoelectric thin film provided by this invention has a surface with parallel, periodically repeating wavy folds; the surface of the anisotropic piezoelectric thin film also has a porous structure, forming a network of interconnected pores. The anisotropic piezoelectric thin film provided by this invention has a regular structure with high order and anisotropy, simulating the parallel arrangement of nerve fiber bundles in vivo, exhibiting good biomimicry, and can effectively guide the directional regeneration of nerves. Using the anisotropic piezoelectric thin film provided by this invention for drug loading and nerve repair combines the effects of physical guidance, release of biochemical factors, and electrical stimulation, effectively promoting the directional growth of nerve axons and the regeneration and repair of nerve tissue.

The utility model provides a multi-electrode cell electrical stimulation device which comprises a multi-electrode plate, the multi-electrode plate comprises a flexible substrate and electrodes, the flexible substrate is provided with a first electrode area and a second electrode area, six electrodes are respectively arranged in the first electrode area and the second electrode area, one end of each electrode is provided with a wire connecting end, and the other end of each electrode is provided with a wire connecting end. The wire connecting end is connected with an external wire, the external wire is communicated with the wire connecting end to complete access operation of the electrode operation end, the multi-electrode plate, the resistor and the waveform function generator are sequentially connected in series to form a series electric control circuit, and the two ends of the series electric control circuit are connected with an external power source to form the electrical stimulation device. Two rows of twelve electrode arrays are arranged, multi-path and multi-direction electrical stimulation scheme adjustment is achieved by controlling the electrodes on the two sides to discharge, growth of nerve axons can be promoted, re-myelination of the axons can be promoted, recovery of nerve functions is accelerated, and popularization and application are facilitated.

The invention belongs to the technical field of medicines, and particularly discloses application of a secretory protein derived from Ackermann muciniphila in preparation of a medicine for treating and / or repairing spinal cord injury, so that the secretory protein (AmTARS) derived from Ackermann muciniphila can effectively repair and / or treat the spinal cord injury. The treatment medicine disclosed by the invention can effectively promote physiological and functional recovery after spinal cord injury; regeneration and germination of nerve axons are promoted, neurons are prevented from being reduced after spinal cord injury, and the integrity of nervous tissue structures is enhanced, so that the overall repair efficiency is improved; the therapeutic drug can be massively expressed and synthesized by using a biosynthesis method, is mature in preparation process, stable in quality, convenient for standardized production, easy to obtain, safe and economical, and has a good application prospect in the aspect of treating and / or repairing spinal cord injury.

The application discloses application of a compound INF195 in treatment of diseases related to nerve axon injury. 17 H 22 ClNO3.The application proves through research that the compound INF195 can significantly promote axon regeneration of DRG neurons at a concentration of 5 muM, and has no significant effect on neuron activity at the concentration.Further based on an axon injury model of Xona microfluidic chip, it is proved that the INF195 can promote regeneration and extension of axons after injury.Therefore, the compound INF195 can inhibit NLRP3 inflammasome activity, and is an important compound molecule for promoting axon regeneration and repair after nerve injury, and has a good application prospect in preparation of drugs for treating diseases related to nerve axon injury, including peripheral nerve injury and central nerve injury.

A double-layer fiber structure is constructed by adopting an electrostatic spinning technology, inner-layer nanofibers are orderly arranged in the axial direction of the catheter to induce directional growth of nerve axons, and outer-layer random fibers provide mechanical support. Collagen particles entrapping nerve growth factors (NGF) are deposited on the inner wall of the catheter through an electrostatic spraying technology, and initial rapid biological activity activation is achieved. Meanwhile, GelMA hydrogel with a cross-linking density gradient is prepared by using a gradient photomask technology, NGF and Schwann-like cells (dSCs) which are subjected to in-vitro induced differentiation are loaded, one-way gradient release and continuous supply of growth factors are realized through gradual degradation of the hydrogel, and a sequential process of in-vivo regeneration is simulated. Structural bionic design, space-time precise release, biological anti-oxidation protection and cell therapy are creatively integrated, an engineering strategy for systematically solving the PNI repair problem is provided, and remarkable innovativeness and wide clinical application potential are achieved.

The invention provides a preparation method of a nerve and tumor interaction model, the nerve and tumor interaction model and application, and relates to the technical field of bio-manufacturing, the preparation method of the nerve and tumor interaction model comprises the following steps: simulating extension nerve axons in ganglions in vivo according to the biological characteristics of nerve and tumor interaction, creating a three-dimensional structure corresponding to the 3D culture model of the neurons and the tumors according to the biological behaviors of the neurons and the tumor tissues and the spatial position relationship between the neurons and the tumor tissues; adding neuronal cells, tumor cells, tumor microenvironment cells and growth factors required by cell growth into the matrix to obtain the bio-ink; and according to the three-dimensional structure corresponding to the 3D culture model of the neurons and the tumors, simulating the spatial position relationship between the neurons and tumor tissues, and carrying out 3D model printing by using the bio-ink to obtain a nerve and tumor interaction model.

The application discloses a silk fibroin / hyaluronic acid interpenetrating network self-adaptive hydrogel support and a preparation method thereof, and belongs to the technical field of medical supplies. The SF / HA interpenetrating network biomimetic hydrogel is developed by using an ice crystal template method in combination with a freeze-drying technology, a microtubule array biomimetic microstructure and a compressive modulus distribution changing in a gradient manner along a radial direction are constructed, and nerve tissue extracellular matrix is fully simulated and nerve axon directional growth is induced. Through a 'biological marker precise response, dynamic coordination bond dissociation and penetration-diffusion mechanism', biological active substances are sequentially delivered, and a regenerative microenvironment is precisely and dynamically regulated. The intelligent biomimetic hydrogel with the optimal comprehensive performance is screened out, and is expected to be prepared in an engineering mode.

The invention discloses application of IL-34 in preparation of a medicine for improving secondary brain injury after cerebral hemorrhage. The exogenous supplement of IL-34 can effectively reverse the attenuation of CSF1R signals after ICH, and recover the steady state of microglial cells. The intervention measure can significantly reduce myelin sheath and nerve axon injury, and significantly improve post-ICH neurological impairment, including improvement of motor coordination ability and spatial memory ability. The IL-34 intervention provides a new therapeutic target for neuroprotection and functional recovery after ICH. Compared with the traditional intervention means, the method targets the IL-34 / CSF1R pathway to reconstruct the steady state of the neural immune microenvironment, and has important theoretical value and wide clinical application prospect. Especially, intranasal administration is used as a non-invasive delivery mode, and high feasibility is provided for clinical transformation of the therapy.

The invention provides an application of Dubosiella Newyorkensis in preparation of a medicine for promoting nerve regeneration after peripheral nerve injury. The Dubosiella Newyorkensis can be prepared into an oral liquid preparation. Experiments show that the bacterial strain can improve the barrier function of the intestinal tract after injury, significantly down-regulate the expression of proinflammatory factors, and activate regeneration promoting signal channels such as p-Akt / Akt and beta-catenin, thereby effectively promoting nerve axon regeneration and motor function recovery. The invention provides a brand new microbial therapy strategy for repairing the peripheral nerve injury.

The application discloses a method for regulating central nervous axon regeneration and motor function recovery by using Rab5 protein, and provides a new target of Rab5 protein for promoting central nervous axon regeneration and function recovery. When the Rab5 protein in the application is overexpressed in vivo, the axon regeneration and function recovery of the central nervous can be significantly promoted, and vice versa, the axon regeneration and function recovery of the central nervous can be inhibited.