PLGA three-dimensional nerve conduit and preparation method thereof

A technology of nerve conduit and PLGA, which is applied in the fields of pharmaceutical formulation, fiber treatment, coating, etc., can solve the problems of unfavorable cell penetration and three-dimensional growth, small pore size, and low porosity of nano-scale fibers, so as to promote cell adhesion and axis The effects of sudden growth, easy degradation, and excellent biomechanical properties

Active Publication Date: 2016-11-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nanoscale fibers prepared by traditional electrospinning technology have low porosity and small pore size, which is not conducive to cell penetration and three-dimensional growth.

Method used

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  • PLGA three-dimensional nerve conduit and preparation method thereof
  • PLGA three-dimensional nerve conduit and preparation method thereof
  • PLGA three-dimensional nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Weigh 1.5 g of PLGA with an electronic balance and dissolve it in 10 mL of hexafluoroisopropanol, stir overnight until completely dissolved to obtain a PLGA spinning solution; use a custom-made double-nozzle spinning equipment to prepare PLGA yarns with uniform diameters, spin The silk parameters are: the flow rate of the PLGA spinning solution of the double nozzles is 0.02mL / min and 0.032mL / min, the two ends are positive high voltage and negative high voltage +10kV and -10kV respectively, the distance between the two nozzles is 15cm, and the rotating horn The rotating speed 500rpm, the distance from the bottom end of the rotating horn to the yarn receiving device is 60cm, and the rotating speed of the yarn receiving device is 5rpm to obtain the PLGA yarn; the PLGA yarn is cut into a uniform length of 8cm, and a receiving rod with a diameter of 2mm is selected. Arrange 40 yarns axially on the receiving rod and fix them; use this as a rotating device to receive PLGA fibers...

Embodiment 2

[0038] Weigh 1.2 g of PLGA with an electronic balance and dissolve it in 10 mL of hexafluoroisopropanol, stir overnight until completely dissolved to obtain a PLGA spinning solution; use a custom-made double-nozzle spinning equipment to prepare PLGA yarns with uniform diameters, spin The silk parameters are: the flow rate of the PLGA spinning solution of the double nozzles is 0.02mL / min and 0.032mL / min, the two ends are positive high voltage and negative high voltage +10kV and -10kV respectively, the distance between the two nozzles is 15cm, and the rotating horn The rotating speed of 350rpm, the distance from the bottom end of the rotating horn to the yarn receiving device is 60cm, and the rotating speed of the yarn receiving device is 7rpm to obtain the PLGA yarn; the PLGA yarn is cut into a uniform length of 10cm, and a receiving rod with a diameter of 1mm is selected. Arrange 20 yarns axially on the receiving rod and fix them; use this as a rotating device to receive PLGA f...

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Abstract

The invention relates to a PLGA three-dimensional nerve conduit and a preparation method thereof. The PLGA three-dimensional nerve conduit comprises a laminin coating and an orientation yarn core layer. The preparation method comprises the steps that a polylactide acid-glycolic acid copolymer (PLGA) is dissolved into a solvent, and the materials are mixed to be uniform to obtain a spinning solution; single-spray-head electrostatic spinning is conducted to obtain the PLGA three-dimensional nerve conduit containing the orientation yarn core layer, the PLGA three-dimensional nerve conduit is bonded with laminin through covalent bonds, and then the PLGA three-dimensional nerve conduit which is provided with the laminin coating and contains the orientation yarn core layer is obtained. The three-dimensional nerve conduit which is provided with the laminin coating and filled with orientation yarn has the good mechanical property, biological activity and degradation property; protein in the coating can promote nerve cell adhesion and nerve axon regeneration, and meanwhile, the yarn in the core layer can induce nerve cells to grow in a crawling mode in the yarn orientation direction, so that regeneration and reconstruction of three-dimensional nerve tissue are promoted, and important application can be achieved in peripheral nerve tissue repair and regeneration.

Description

technical field [0001] The invention belongs to the field of nerve guide and its preparation, in particular to a PLGA three-dimensional nerve guide and its preparation method. Background technique [0002] Electrospinning technology has a very important application in the field of preparation of tubular tissue engineering scaffolds. A variety of synthetic polymer materials, natural polymer materials and their blends can be used in electrospinning technology to prepare fibers from nanometer to micrometer stand. The fiber scaffold prepared by electrospinning technology can simulate the natural extracellular matrix components from the selection of raw materials, and the fiber structure scaffold can also mimic the structure of the natural extracellular matrix, with a high specific surface area, which is conducive to cell adhesion and proliferation. However, nanoscale fibers prepared by traditional electrospinning technology have low porosity and small pore size, which is not c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/18A61L27/58A61L27/50D01D5/00
CPCA61L27/18A61L27/34A61L27/50A61L27/58A61L2400/18A61L2430/32D01D5/003D01D5/0069D01D5/0076D01D5/0092C08L89/00C08L67/04
Inventor 莫秀梅吴桐王元非李丹丹孙彬彬
Owner DONGHUA UNIV
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