39 results about "Nerve guide" patented technology

The present invention is directed to the compositions and methods of preparing hydrogel-grafted nerve guides for peripheral nerve regeneration. Particularly, the present invention describes the nerve guides and methods for preparation of hydrogel-grafted nerve guides with encapsulated neurotrophic factors and a nanofiber mesh lining the inner surface of the guide. The present invention also provides methods for peripheral nerve repair using these hydrogel-grafted nerve guides.

A biological nerve guide for implantation into a human body is made by providing a natural animal tissue membrane, crosslinking and fixing the membrane, minimizing the antigens from the membrane, tanning the membrane, coupling an active layer to an inner surface of the membrane, cutting the membrane into a desired shape and size, positioning the cut membrane onto a rod-shaped mold so that the cut membrane assumes a cylindrical configuration, and attaching a spiral support to the outer surface of the cut membrane.

The aim of the invention is to provide a nerve guide that allows the axons to develop fairly freely during regeneration. For this purpose, the nerve guide is produced based on a shaped body from a cross-linked, resorbable, gelatin-based material. The shaped body is a tubular hollow body having a wall with an exterior surface and an interior surface, which wall defines a lumen. The nerve guide comprises a semipermeable layer surrounding the lumen.

The invention provides for methods for delivering a nucleic acid into a peripheral neuron by identifying a target neuron in a dorsal root ganglion and intrathecally delivering a vector comprising the nucleic acid to the dorsal root ganglion neuron. The nucleic acid may encode a neurotrophic factor that may be used to treat a peripheral neuropathy or, in conjunction with a nerve guide conduit, to treat a transected peripheral nerve.

The invention discloses a nerve guide and a preparation method thereof. The nerve conduit comprises an inner layer, an outer layer and at least one micro-cavity for storing bioactive factor solution, the inner layer is a hydrophilic cell support layer, and the outer layer is a hydrophobic nerve guide support layer. The nerve guide may also comprise a transition layer between the inner and outer layers. The preparation method of the nerve guide is to firstly prepare the inner layer by electrospinning, then add the material for preparing the cavity, and then prepare the outer layer, and after taking out or dissolving the material for preparing the cavity, the nerve guide can be obtained. The nerve guide has a micro-cavity, which can be loaded with biologically active factors according to the need before the operation, such as injection, soaking, etc., which not only makes the production quality of the product easy to control, but also greatly improves the survival rate of the biologically active factors, and more efficiently promotes Nerve regeneration and enhanced nerve repair effect.

The invention relates to the field of biomedical engineering, and discloses an axial gradient protein-loaded silk nerve guide and its preparation and application. In the present invention, by grafting o-nitrobenzyl photosensitive molecules on silk fibroin, silk fibroin modified by o-nitrobenzyl photosensitive molecules is obtained, and then prepared into a photoactive silk nerve guide, and finally The silk nerve guide is soaked in the nerve growth factor solution, the front end, the middle part and the end are irradiated with blue light along the axial direction, after washing and freeze-drying, an axial gradient protein-loaded silk nerve guide is obtained. The invention gradually reduces the light irradiation time in the front, middle and end regions of the silk nerve guide, and realizes the precise and controllable gradient loading of the silk nerve guide. The biological activity of the silk nerve guide of the invention is greatly improved, can effectively promote the proliferation and migration of nerve cells, can induce the rapid growth of new nerve tissue, and achieve good nerve repair effect in clinical practice.