19 results about "Micro injection" patented technology

The invention provides a grouper microinjection method, and belongs to the technical field of aquaculture. According to the method, grouper eggs are injected by utilizing a grouper microinjection needle, the microinjection needle is prepared by calcining a glass capillary tube by utilizing a needle pulling instrument, and the parameters of the needle pulling instrument are set as P = 500mW, HET = 720ms, PULL = 60, VEL = 20mu m / s and TIME = 250ms; the outer diameter of the glass capillary tube is 1.0 mm, the inner diameter of the glass capillary tube is 0.75 mm, and the length of the glass capillary tube is 10 cm. The method for injecting the grouper eggs with the micro-injection needle is simple and easy to operate, exogenous gene transfer and endogenous gene accurate editing of the grouper can be realized by adopting the method disclosed by the invention, the target gene editing efficiency is high, and powerful support is provided for deeply researching gene functions of the grouper and cultivating excellent culture varieties.

The utility model discloses a trace liquid sample storage and taking container which comprises a sample storage unit for directly containing a liquid sample, a sample taking unit arranged at the input end of the sample storage unit, and an injection head protection unit arranged at the output end of the sample storage unit, the sample storage unit comprises a first columnar base body, a sample storage cavity and a micro injection pipe. According to the utility model, the principle of an injector is ingeniously applied, a split detachable connecting system is designed, and all units form a closed container after being assembled, so that the sample transportation is convenient; the technical defect of liquid residue in the traditional taking process is eliminated by adopting a sampling mode of pushing out a micro sample from a micro injection pipe below the sample storage cavity, so that the utilization rate of the sample is improved; in addition, a bucket-shaped input opening is formed in the upper portion of the sample storage cavity, so that sample injection is facilitated.

The invention discloses a method for multi-dimensional uniform deformation of a micro-nano structure, belongs to the technical field of advanced material processing and manufacturing, and solves the problems of product structure distortion and out-of-control precision of a nano-scale structure caused by low shrinkage rate and non-uniform sintering process densification in the prior art. The method comprises the following steps: uniformly mixing matrix powder, an activator and a morphology stabilizer to obtain a mixture, and carrying out micro-injection molding on the mixture to obtain a pre-amplified micro-nano structure; performing segmented degreasing on the pre-amplified micro-nano structure, performing gradient sintering, and performing hot isostatic pressing treatment to obtain a multi-dimensional uniformly-deformed micro-nano structure; wherein the matrix powder comprises metal powder and / or ceramic powder, the activating agent is eutectic alloy with the melting point smaller than or equal to 200 DEG C, and the morphology stabilizing agent is rare earth metal oxide and / or transition metal oxide. The invention has the advantages of high shrinkage rate, uniform shrinkage and high mechanical strength.

A micro injection assembly for the repair of floors that includes an adapter body operably configured to couple with a tube having adhesive and defining an internal channel, a hub selectively removably coupled to the adapter body and having a rigid needle member coupled thereto and recessed within the hub, wherein the rigid needle member projects outwardly from the second end of the hub in a linear orientation, has a blunt distal end, and defines a needle channel spanning the length of the needle member. The assembly also includes a cap selectively removably couplable to the adapter body in a watertight configuration and with the cap disposable in an overlapping configuration with the adapter body. The assembly is operable to effectuate emission of an adhesive material from a tube through the adapter body, hub, and needle member to spaces between a flooring material and subfloor.

The invention relates to the technical field of assisted reproduction, and discloses a microinjection device and an operation method, the microinjection device comprises a handle, a rotary vibration shaft, a piezoelectric vibrator and a micromanipulation needle, the piezoelectric vibrator is tangentially arranged between the handle and the rotary vibration shaft, and electric energy is converted into circumferential high-frequency micro vibration of the rotary vibration shaft. The circumferential vibration is transmitted to the micromanipulation needle connected with the front end, so that the unique fish-mouth-shaped end face of the micromanipulation needle penetrates through the cells in a high-frequency rotary cutting mode, and extrusion damage to the oocytes caused by traditional axial puncture is remarkably reduced. The invention further discloses a gallium-based alloy which is high in density and better in biocompatibility and serves as an operation medium, and the transmission efficiency of vibration energy is improved. The invention also provides a method for pre-filling the microinjection needle by utilizing the physical characteristics of low-temperature curing and normal-temperature liquefaction of the gallium-based alloy and adopting cold chain transportation, so that the product can be used immediately after being opened, the clinical operation steps are obviously simplified, the operation threshold is reduced, and the microinjection efficiency and success rate are improved.

The application discloses a memristor, a method for preparing the same and an artificial synapse device, and the memristor comprises a glass cone and a polyelectrolyte structure on the inner wall of the glass cone; the glass cone has a first end opening and a second end opening arranged oppositely, the diameter of the first end opening is smaller than that of the second end opening, and the polyelectrolyte structure is located on the inner wall of the first end opening. Thus, the memristor has low power consumption of a picojoule level, a low working potential and high efficiency. When a pulse potential stimulus is applied, the memristor can generate a double-pulse facilitation and a double-pulse inhibition effect similar to a neuron synapse, and due to the existence of an ion effect, the effective regulation of the plasticity of the system can be realized. Further, the signal transduction function based on ions can be realized by being combined with a micro-injection system. In addition, the memristor has excellent stability and designability and the like.

The micro injection molding structure without hot nozzle heating comprises a splitter plate, the splitter plate comprises a diffusion welding splitter plate main body, the diffusion welding splitter plate main body is provided with a diffusion welding runner used as a small-spacing runner, the diffusion welding runner is communicated with a pouring gate, and the pouring gate is matched with a nozzle core module for use; a heating wire is arranged on the diffusion welding splitter plate main body to form a self-heating splitter plate structure, and the splitter plate structure is matched with the nozzle core module to form a micro injection molding structure without a conventional hot nozzle heating structure.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

This invention discloses a micro-injection mold with an adjustable frequency amplitude combined variable angle vibration flow channel structure, including a sprue bushing disposed in a fixed mold plate; the sprue bushing includes two oppositely arranged arc-shaped blocks, and a mechanical vibration block and an ultrasonic vibration block oppositely arranged between the two arc-shaped blocks; the arc-shaped blocks are fixed to the fixed mold plate; the mechanical vibration block and the ultrasonic vibration block are multiple blocks, each stacked layered between the two arc-shaped blocks; the mechanical vibration block, the ultrasonic vibration block, and the two arc-shaped blocks enclose an internal region that is a through flow channel; the flow channel has a variable angle structure, and from the inlet of the sprue bushing to the cavity, the inclination angle of the inner surfaces of the stacked mechanical vibration block and ultrasonic vibration block gradually changes, causing the cross-sectional area of ​​the flow channel to gradually increase. The mechanical vibration block and the ultrasonic vibration block are respectively connected to a vibration source, applying increased vibration to the polymer melt, reducing the viscosity of the polymer melt during injection molding, and improving fluidity and filling capacity.

The utility model belongs to the technical field of microinjection, and particularly relates to a single cell microinjection device which comprises a rotating shaft, a mounting frame is mounted at one end of the rotating shaft, rotating shafts are rotatably mounted on two sides of the mounting frame in a penetrating manner, a locking screw is rotatably mounted in one end of a side plate, and a gear groove is mounted at the other end of the rotating shaft. A clamping block is assembled at the bottom end of the limiting rod, and an extension spring is fixedly connected between the limiting block and the supporting plate. A limiting block is lifted upwards, a limiting rod moves upwards, an extension spring is stretched, a clamping block is separated from a gear groove, a rotating shaft drives a mounting frame to rotate, the angle of the injector in the X-axis direction is changed, the angle of the injector in the Y-axis direction is adjusted, a locking screw releases fixation of a rotating shaft, and the injector is rotated through rotation of the rotating shaft; therefore, the angle of the injector can be adjusted at will, the injector can be aligned with target cells more flexibly in the micro-injection process, and the injection effect is improved.

The application provides an ultrasonic plasticizing micro injection molding equipment for producing a micro cantilever beam and a use method, relates to ultrasonic plasticizing molding, and comprises a movable mold fixing plate, a fixed mold fixing plate, a base plate, a protective sleeve, a mold assembly and an ultrasonic head. The application utilizes the base plate to form a centering part, the outer profile of the centering part and the inner profile of the protective sleeve are both inclined surfaces, the centering part and the protective sleeve are centered by utilizing the principle of inclined surface fitting, the blanking pipeline formed by the protective sleeve is fitted with the injection hole surface of the mold assembly to realize the centering of the protective sleeve and the mold assembly, the centering efficiency in the ultrasonic plasticizing micro injection process is improved, and the centering difficulty is reduced. Meanwhile, the mold core unit is also improved, the mold core base and the micro structure cavity are designed in a split mode, different stiffness micro structures are conveniently generated, and the forming length of the micro cantilever beam is increased by means of two side feeding.

The application relates to the technical field of microinjection, in particular to a microinjection system and an injection method. The microinjection device comprises a moving carrier, at least two mechanical arms and injectors arranged at the ends of the mechanical arms correspondingly. The moving carrier comprises a horizontal moving platform and a rotating platform. The rotating platform is arranged on the horizontal moving platform and is used for carrying culture dishes containing at least two different types of samples. The rotating axis of the rotating platform is arranged vertically. The horizontal moving platform is used for driving the rotating platform to move in the mutually perpendicular horizontal X direction and horizontal Y direction. The number of the injectors is the same as the number of the sample types. The at least two mechanical arms drive the injectors at the ends to perform injection operations on one type of sample. The application can perform injection on various types of samples in the same culture dish, thereby greatly improving the efficiency of microinjection operation.

The utility model discloses an auxiliary device for microinjection, which comprises an injection table serving as a mounting carrier, a cylinder bin is mounted in the center of the top of the injection table, six groups of screw rods are arranged in the injection table in a central symmetry manner, and inner threaded blocks are arranged on the outer sides of the screw rods in a threaded manner. An outer sleeve is mounted at the top of the internal thread block, and a fixing pipe is arranged in the outer sleeve. According to the device, the driving device is controlled to drive the six groups of screw rods to synchronously rotate, so that the internal thread blocks are forced to drive the outer sleeves to move towards the central position of the cylindrical bin, and a culture dish positioned in the cylindrical bin is jointly pushed to the central position in the cylindrical bin through one ends of the six groups of outer sleeves and is clamped and fixed; as the six groups of outer sleeves can freely and synchronously move, culture dishes with different sizes can be fixed and automatically pushed to the central position of the bottom in the cylindrical bin, so that the convenience of the microinjection equipment in the use process is improved.

The utility model belongs to medical storage technical field, concretely speaking is a kind of microinjection needle storage device, including lower box and upper box, the upper box one side is hinged on the one side of lower box, and is opened and closed by opening and closing piece to carry out opening and closing operation, the lower box both sides wall all are fixed with multiple sets of locating plate, the locating plate between adjacent is equipped with placing plate by inserting piece, the placing plate top end is equipped with oval slot, the oval slot in fixed attachment has foam pad, the utility model provides a kind of microinjection needle storage device, to the problem of existing microinjection needle needle body storage inconvenience.

The invention belongs to the technical field of cell biology, and particularly discloses a marine fish gene editing and mutant screening method which comprises the steps of sgRNA synthesis, Cas9-sgRNA RNP complex preparation, microinjection and mutant screening. The marine fish microinjection needle and the biological glue are used, so that the problem that microinjection of marine fish fertilized eggs is difficult is solved; on the basis, the mutants can be screened through PCR amplification, HRMA analysis and PAGE electrophoresis, so that the screening cost is greatly reduced; in addition, the mutant screening time is shortened from several weeks to several months to about four days, and an efficient and economical novel method is provided for marine fish gene editing and mutant screening.

The utility model relates to the technical field of embryo micro-injection experimental devices, and discloses a salmon and trout embryo micro-injection pretreatment device which comprises an incubator, the placing assembly comprises a plurality of placing pieces and a plurality of connecting pieces, the connecting pieces are fixedly connected to the incubator in the axial direction, the placing pieces are placed in the incubator in the circumferential direction and detachably connected with the connecting pieces, and the placing pieces are used for storing salmon and trout roes; the temperature control part is arranged on the incubator and used for controlling the temperature in the incubator; and the oxygen control piece is arranged on the incubator and is used for controlling the oxygen content in the incubator. The device meets the requirements of constant low temperature and high oxygen in the operation process, is beneficial to improving the survival rate of subsequent embryo injection, can meet the experimental pretreatment requirements of multiple experimental batches, can effectively ensure the activity of roes before injection, and improves the injection efficiency.

The invention discloses a ceramic microcrack repairing method, and belongs to the technical field of ceramic material repairing. The method comprises a detection process, a multi-mode imager combined infrared thermal imaging technology, industrial CT and ultrasonic phased array detection ceramic piece, microcrack feature extraction, a laser processing process, a micro-hole processing process by adopting a Q-switched fiber laser, a coating process, and a micro-injection technology filling brazing filler metal into the micro-hole. And a pulse pressure gauge is adopted to compact the brazing filler metal. According to the method, the three-dimensional space characteristics of the microcracks can be accurately extracted by arranging the multi-mode imager and combining the infrared thermal imaging technology, the industrial CT technology, the ultrasonic phased array technology and the focused ion beam imaging technology, the depth extraction error is small, the problems that in the prior art, microcrack parameter extraction is incomplete, and the error is large are solved, and based on the accurate data, the accuracy of microcrack parameter extraction is improved. Laser processing parameters can be dynamically matched with the sizes of the micro-cracks, and stress concentration can be effectively avoided through the processed arc-shaped bottom blunt holes.

This invention relates to a method and system for tracking rare sperm based on microscopic visual servoing and spatiotemporal feature fusion. The system includes a microscopic scanning and hardware closed-loop module, a spatiotemporal fusion recognition and multi-target tracking module, and a visual-control collaboration and system control module. In coarse scanning mode, the system performs a rapid, large-field-of-view search. When the recognition module accurately detects a suspected sperm in a complex, unstained background using spatiotemporal feature fusion, an interrupt is immediately triggered, seamlessly switching the system to a fine-scanning mode, and automatic focusing is performed based on image clarity. Subsequently, a closed-loop feedback control algorithm is used to dynamically adjust the motion platform for visual servo tracking, combining global motion compensation to construct a long-term motion trajectory in an absolute coordinate system, and calculating kinematic parameters. The system can trigger an alert after target capture and supports manual intervention to choose between continuous tracking or guiding a microinjection needle for sperm aspiration.

According to the hucho taimen roe micro-injection device and method, an injection pipe and a discharge pipe are arranged side by side and integrally manufactured, needle tips are arranged at the lower ends of the injection pipe and the discharge pipe, and a liquid storage tank is communicated with the discharge pipe through a connecting pipe. The method comprises the following steps: 1, semen taking; 2, healthy roes are taken, and a container containing the roes is placed on the ice-water mixture; 3, mixing 1000 + / -10 eggs with the seminal fluid, gently and uniformly stirring, and slowly adding water until the eggs are submerged; 4, standing for 2 minutes, washing the eggs with water at 10 DEG C, and removing redundant sperms; 5, injecting clean water again; 6, pouring 1.2% sepharose gel into a culture dish, and after the sepharose gel is coagulated, poking out a semicircular groove with the diameter of 4-5mm; seventhly, the fertilized eggs absorb water to expand, within 0-15 minutes after meeting water, the fertilized eggs are fixed in the semicircular grooves, and injection is conducted through a micro-injection device; 8, timely picking out the eggs which are damaged, severely leaked, not fertilized or have water mildew infection symptoms; and 9, incubating the fertilized eggs after injection. The microinjection device is used for microinjection of hucho taimen fertilized eggs.