This invention discloses an automated
cell manipulation method and
system guided by
artificial intelligence, comprising: identifying and locating the
biological target object to be manipulated based on real-time images acquired by a
microscopic imaging system to obtain target position information; calibrating and tracking the end-
effector position of a
micromanipulator based on the real-time images to obtain the real-time end-
effector position; planning and dynamically correcting the motion trajectory of the
micromanipulator based on the target position information and the real-time end-
effector position until the end-effector reaches the preset target operation position, and controlling the
micromanipulator to execute a preset interactive operation; verifying the operation result based on the real-time image after the operation to obtain the
verification result, and controlling subsequent processes based on the
verification result. This invention overcomes the inherent defects of traditional manual operation and semi-automated solutions in microscale
cell manipulation through an end-to-end intelligent process, achieving high-precision, high-
throughput, high-success-rate, and highly standardized
cell manipulation.