20results about How to "Accurate quantitative detection" patented technology

The invention provides a dry chemical reagent tablet as well as a preparation method and application thereof, and relates to the field of dry chemical reagent tablets. The dry chemical reagent piece sequentially comprises an upper outer frame, a diffusion layer, a reagent layer, a light-transmitting layer and a lower outer frame from top to bottom, the upper outer frame is provided with a sample adding hole, the lower outer frame is provided with a light-transmitting hole, the reagent layer comprises a reagent layer reagent or is formed by the reagent layer reagent, the diffusion layer comprises a diffusion layer reagent or is formed by the diffusion layer reagent, and the light-transmitting layer is formed by the diffusion layer reagent. The diffusion layer reagent comprises at least one of a hydrophilic high-molecular polymer and an adhesive, the diffusion layer reagent further comprises particulate matters, and the particulate matters comprise first particulate matters and second particulate matters; the number ratio of the first particulate matters to the second particulate matters is 1: (0.25-4); the ratio D2 / D1 of the particle size D2 of the second particulate matter to the particle size D1 of the first particulate matter is 0.2-0.8. The dry chemical reagent sheet provided by the invention is simple and convenient to operate, and can rapidly, accurately and quantitatively detect the contents of various substances in a liquid sample.

The invention provides a hybrid creatine kinase isoenzyme double-antibody sandwich ELISA kit and a preparation method thereof, and belongs to the technical field of CK-MB antibody and creatine kinase isoenzyme quantitative detection. The process comprises the following steps: respectively immunizing Japanese big-ear rabbits and mice by using CK-MB heterodimer protein as an immunogen, screening positive monoclonal cells by adopting a Beacon single-cell screening platform, and preparing a rabbit (mouse) anti-human CK-MB gene engineering antibody; the double-antibody sandwich ELISA detection kit is established by further screening optimal reaction conditions. The detection method disclosed by the invention has excellent performance in the aspects of intra-batch and inter-batch repeatability, sensitivity and specificity, and is suitable for CK-MB detection in clinical and scientific research.

The invention provides application of nano CDs as a fluorescent probe in fluorescence detection of sugammadex sodium and a fluorescence detection method of sugammadex sodium, and relates to the technical field of drug analysis and detection. According to the invention, carboxyl in sugammadex sodium and nitrogen atoms in nano CDs interact with each other, the fluorescence intensity of nano CDs is enhanced through intramolecular charge transfer, sugammadex sodium and nano CDs can be effectively combined through a static combination effect, and nano CDs have high sensitivity and selectivity to sugammadex sodium; the method is suitable for detecting sugammadex sodium. By adopting a fluorescence analysis method, the high-sensitivity, high-selectivity, simple, rapid and accurate quantitative detection of the sugammadex can be realized according to the fluorescence enhancement efficiency of the sugammadex relative to the nano CDs after the sugammadex is added and a standard curve of the concentration of the sugammadex and the fluorescence enhancement efficiency. The fluorescence detection method provided by the invention is low in detection cost.

The invention relates to a microfluidic electrochemical aptamer sensor and a microfluidic electrochemical analysis system. According to the invention, a series of important biomarkers such as adenosine, homocysteine and the like in a blood sample of an organism can be rapidly and accurately quantitatively detected, so that the health condition of the organism can be judged for subsequent treatment. In addition to instant and rapid detection, the device is moderate in size and weight, convenient to carry and suitable for detecting the body health condition in various environments such as hospitals, families and fields, and the obtained physiological parameters are used for giving an early warning to the health condition of people.

The present invention belongs to the field of analysis and detection, and specifically relates to a method for simultaneously detecting residual organic solvents of N,N-dimethylformamide and sulfolane in amifostine by gas chromatography, which includes sample pretreatment and gas chromatograph detection method, and the steps are as follows: take amifostine, first add water to completely dissolve it, then add an organic solvent to filter after amifostine is precipitated, and take the filtrate and directly enter the gas chromatograph according to the gas chromatography detection conditions. The sample pretreatment method of the present invention can not only completely dissolve the DMF and sulfolane wrapped in the amifostine sample, but also remove the high-boiling point amifostine and directly enter the gas chromatograph, avoiding the contamination of the gas chromatograph and the detection interference caused by the degradation impurities of amifostine. The gas chromatograph detection method provided by the present invention can simultaneously and accurately detect the content of residual organic solvents of N,N-dimethylformamide and sulfolane in amifostine.

The application discloses Au@PATP@Ag NRs nanomaterial and a preparation method and application thereof. The application also discloses a method for qualitatively and quantitatively detecting nicotine concentration based on surface enhanced Raman scattering technology and application thereof. The Au@PATP@Ag NRs nanoparticles are used as active base materials of SERS, and a target object is analyzed and detected by using a Raman spectrometer. According to a linear relation equation of a ratio of SERS signal intensity of different concentrations of nicotine solution to characteristic peaks of nicotine and an internal standard substance, the content of nicotine in a solution to be detected can be obtained, and the detection sensitivity of nicotine is greatly improved. The internal standard molecule is introduced into the core-shell structure, and the accuracy of quantitative detection of nicotine is improved. The application also has great application in sensitive detection of nicotine in cigarettes and environmental cigarette smoke. In addition, the application also has the advantages of simple principle, rapidness, simple pretreatment, high selectivity, high detection sensitivity and the like.

A device and method for detecting the pull-out force of planetary gear shafts in automatic transmissions belong to the technical field of automotive automatic transmissions. The device includes a base, a gantry bracket, replaceable testing support fixtures, a pressure detector, a testing platform, a pressure sensor, a hydraulic cylinder, replaceable pressure heads, guide columns, and a hydraulic mechanism. All components are rigidly connected. The fixtures and pressure heads are interchangeable to accommodate different specifications of planetary gears. The hydraulic mechanism supplies pressure to the hydraulic cylinders, and the pressure sensor collects force values. The testing method includes four steps: fixture adjustment, placement and positioning, pull-out detection, result judgment, and completion. Hydraulic force is applied to press the shaft, the sensor collects data, and the detector performs judgment, storage, and data export. This invention, through multi-system collaborative design, achieves accurate and quantitative detection of the pull-out force at the riveting joint of the planetary gear shaft. It combines versatility, convenience, and data traceability, effectively solving many shortcomings of existing testing methods and providing reliable assurance for riveting quality control.

The invention discloses a method for detecting pesticides, heavy metals and active ingredients of traditional Chinese medicinal materials, and relates to the technical field of traditional Chinese medicinal material detection. The method comprises the steps of pretreatment of the traditional Chinese medicinal materials, preparation of liquid medicine, detection of the liquid medicine and post-treatment of medicine residues of the liquid medicine. A self-made multifunctional solid-phase extraction column is combined for purification treatment, and the multifunctional solid-phase extraction column can effectively remove impurities in supernate through reasonable silica gel, Flory silica and activated carbon filling combination and filling amount setting, so that the enrichment and selective separation effects on target pesticide components are improved; meanwhile, accurate detection of various pesticide residues in the traditional Chinese medicinal materials can be achieved, especially the trace pesticide residues can also reach a low detection limit, the increasingly strict pesticide residue limit standard requirement is effectively met, and the problem that an existing detection method is insufficient in sensitivity is solved.

The invention provides a multiplex PCR (Polymerase Chain Reaction) micro-fluidic chip integrating electric cracking and nucleic acid purification detection and application thereof. The multiplex PCR micro-fluidic chip comprises a PDMS (Polydimethylsiloxane) layer and a glass substrate which are connected together in an ionic bonding manner, wherein the PDMS layer is internally provided with a cracking cavity, a first washing cavity, a second washing cavity and an amplification cavity which are sequentially communicated through a micro-channel at the bottom; a silver paste interdigital electrode is printed on the glass substrate through a screen printing technology, the silver paste interdigital electrode is aligned with the cracking cavity below the cracking cavity, and after a power supply is switched on, a strong electric field can be generated through current to realize electric cracking of bacteria. The chip is simple in structure, suitable for batch process manufacturing, low in cost and suitable for popularization and application. According to the present invention, the electric splitting method and the microfluidic technology are integrated, such that the splitting and the accurate quantitative detection of the bacteria are achieved, the great advantages in the detection sensitivity, the process automation, the integration level and the like are provided, and the wide application prospect in the molecular diagnosis aspect is provided.

The invention relates to human eye color vision detection, in particular to a human eye color vision defect quantitative detection method and a human eye color vision defect quantitative detection device, which are characterized in that a color resolution limit of a subject is gradually approached by using a time and space two-dimensional alternate change detection pattern in combination with a dichotomy chromaticity threshold interactive feedback test method, and a Mecanum color confusion ellipse of the subject is obtained; inputting the Mecanum color confusion ellipse of the subject into the trained color vision defect type identification model to obtain the color vision defect type of the subject; determining a changed LMS cone spectral response curve according to the color vision defect type of the subject, and obtaining an LMS cone spectral response curve of the subject in combination with the LMS cone spectral response curve of the standard observer; according to the technical scheme provided by the invention, the defect that the human eye color vision defect is difficult to accurately and quantitatively detect in the prior art can be effectively overcome.

This invention belongs to the field of fetoglobulin B detection technology, and discloses an immunochromatographic test strip for quantitative detection of fetoglobulin B using quantum dot fluorescent microspheres and its preparation method. The immunochromatographic test strip of this invention includes a sample pad, a release pad, and a nitrocellulose membrane; the release pad is coated with a fetoglobulin B monoclonal antibody labeled with quantum dot fluorescent microspheres and a mouse IgG antibody labeled with quantum dot fluorescent microspheres; the nitrocellulose membrane has a detection line and a control line, the detection line is coated with fetoglobulin B monoclonal antibody, and the control line is coated with goat anti-mouse IgG antibody. The immunochromatographic test strip of this invention reduces the detection time to 9-12 minutes, and the total detection time is only 20 minutes, achieving rapid and accurate quantitative detection of fetoglobulin B. It also features high detection sensitivity, a wide detection range, and simple and rapid operation, which is beneficial for the screening and monitoring of atherosclerotic coronary heart disease.

This invention discloses a verification sample and verification method for halftone mask HTM repair process, relating to the field of flat panel display mask manufacturing technology. The verification sample includes a substrate, on which are integrated dimensional accuracy testing structures, edge straightness testing structures, seam straightness testing structures, and ZAP repair straightness testing structures. The ZAP repair straightness testing structure comprises two opposing HTM patterns, forming an overlapping area. After being removed by ZAP laser scanning, a third seam is formed for evaluating the repair effect. The halftone layer of the verification sample has a light transmittance of 35%-45%. The verification method includes five steps: dimensional accuracy verification, edge straightness verification, seam straightness verification, ZAP repair effect verification, and transmittance consistency verification. This invention, through the integrated design of four types of testing structures, achieves systematic quantitative verification of the dimensional accuracy, multi-directional straightness, ZAP repair straightness, and optical transmittance of the HTM repair process.

The present invention discloses a POCT detection device and a detection method, wherein the POCT detection device comprises: a base; a magnetic part, arranged on the top of the base; a substrate, arranged above the base and covering the magnetic part. The POCT detection method comprises: S1, preparing detection reagents and the above-mentioned POCT detection device, wherein the detection reagents comprise magnetic microspheres-first monoclonal antibodies and fluorescent microspheres-second monoclonal antibodies; S2, mixing the detection reagents with the sample solution and dripping them onto the position of the substrate corresponding to the magnetic part; S3, measuring the fluorescence intensity on the surface of the substrate to obtain the target content in the sample solution. The POCT detection device of the present invention has the characteristics of simple structure, simple operation and low cost, and the POCT detection method of the present invention has the characteristics of high detection efficiency, high sensitivity and high precision.

The invention belongs to the technical field of heavy metal ion detection, and particularly discloses a preparation method of an ionic liquid modified nanogold cluster material and application of the ionic liquid modified nanogold cluster material in detection of heavy metal ion cadmium. According to the invention, bovine serum albumin is taken as a stabilizing agent and a reducing agent, a nanogold cluster emitting red fluorescence is synthesized, and the ionic liquid modified nanogold cluster composite material is synthesized by utilizing electrostatic interaction between imidazolium ionic liquid and BSA (Bovine Serum Albumin). Due to the unique coordination effect between the cadmium ions and the imidazolium ionic liquid, the cadmium ions can effectively enhance the fluorescence intensity of the ionic liquid modified nanogold cluster composite material, the ionic liquid modified nanogold cluster composite material can be applied to detection of the cadmium ions in an actual sample, and a new thought is provided for convenient detection of the cadmium ions in a complex matrix.

The present invention discloses a novel multifunctional MOF on MOF gel material and its preparation method and application, named FCZE, the gel material is a core-shell structure, with a bimetallic organic framework MOF as the core, a Uio-66 / Eu layer as the shell, the bimetallic is Fe and Cu, Uio-66 and Eu are coordinated and connected in the Uio-66 / Eu layer, and the bimetallic organic framework MOF is first prepared using copper salt, iron salt and trimesic acid; then pyromellitic acid, zirconium salt and europium salt are added to prepare the MOF on MOF gel material. The MOF on MOF gel material prepared by the present invention integrates different types of signal conversion mechanisms (fluorescence and colorimetry) through a unique composite structure design, and realizes rapid and sensitive detection of CTC, CIP and D-PA at the same time.

The invention belongs to the field of interdisciplinary application of lipid catabolism quantitative detection and cell image analysis processing, and particularly relates to quantitative detection of lipid droplet degradation rate in fat cells by means of living cell image quantitative analysis. According to the present invention, with the combination of the microscope imaging and the biophysical modeling method, the method is particularly suitable for the detection of the lipolytic enzyme activity degree and the lipid droplet degradation rate under the condition of the trace cell number (the cell number is less than 100), and is also suitable for the lipolytic detection of the primary mature fat cells. The method is based on general microscope bright field imaging, is simple, does not need chemical reagents or isotope labels required by the existing lipolysis measurement technology, shows the detection advantages of accuracy, quantification and high repeatability, can objectively and accurately provide quantitative analysis measurement of the lipolysis rate of analysis of the single lipid droplet level and the single cell level, and has a good application prospect. Wide application prospects are realized.

This invention belongs to the field of chloride detection technology, specifically relating to a perovskite-based nanomaterial, its preparation method, and its application. The perovskite-based nanomaterial includes upconversion nanomaterials and Ag₂S quantum dots, with the upconversion nanomaterials and Ag₂S quantum dots linked by chemical bonds. The upconversion nanomaterials have a core-shell structure, with Yb 3+ / Er 3+ The perovskite-based nanomaterials form the core, which is then coated with a Cs₂NaYF₆ shell. The perovskite-based nanomaterials are added to the sample, and the emission intensity of the sample is measured in the 300–750 nm range under 980 nm laser excitation. The chloride concentration is calculated based on a standard curve. The perovskite-based nanomaterials prepared in this invention exhibit high detection accuracy in chloride detection, enabling quantitative detection of chloride at the micromolar level. Furthermore, the preparation method is simple and inexpensive.

This invention discloses a method for synthesizing a standard sample for detecting tartrazine intermediates as shown in structural formula 1, characterized by the following reaction formula 1. This patent provides a method for preparing a standard sample for detecting the content of tartrazine intermediates, the structure of which is shown in structural formula 1, enabling rapid and accurate quantitative detection of 1-(4'-sulfonylphenyl)-3-carboxylic acid methyl ester-5-pyrazolone monosodium salt in tartrazine.

The invention provides a method for detecting p-aminophenol in compound paracetamol and chlorpheniramine maleate granules and application, and belongs to the technical field of chemical drug analysis methods. The invention discloses a method for detecting p-aminophenol in compound paracetamol and chlorpheniramine maleate granules, which is high performance liquid chromatography, and the high performance liquid chromatography conditions are as follows: a chromatographic column is an octadecyl silane bonded silica gel chromatographic column; the detection wavelength is 255 to 259 nm; the flow velocity is 0.9 to 1.1 ml / min; the column temperature is 28-32 DEG C; a 0.025 mol / L disodium hydrogen phosphate solution (the pH is adjusted to 6.6 by phosphoric acid) is used as a mobile phase A, methanol is used as a mobile phase B, and gradient elution is performed for separation; the solvent is a 15% methanol water (containing 0.1 mg / ml ascorbic acid) solution. According to the analysis method, p-aminophenol in the compound paracetamol and chlorpheniramine maleate granules can be accurately and quantitatively detected, the separation degree of a p-aminophenol peak and an adjacent impurity peak meets the requirement, and the method is high in specificity, good in durability and high in sensitivity.

The present application provides a kind of self-insulation concrete wall real stone paint flatness image detection method and system, specifically, composite structured light pattern is projected to the wall surface to be measured, the pattern is logarithmic frequency modulation sinusoidal stripe as high-resolution phase carrier, Gray code of two-dimensional absolute position information is superimposed and encoded in chroma channel, deformed pattern image is synchronously collected, frequency response confidence map is generated based on the image, global energy function containing confidence weight and smoothing constraint is constructed, high-precision absolute phase map is obtained by solving, and is converted into initial three-dimensional point cloud, high-fidelity three-dimensional point cloud is obtained by weighted nonlinear optimization, the three-dimensional point cloud is converted into depth height map, is decomposed to the scale corresponding to the detection standard by the two-dimensional discrete wavelet transform associated with physical scale, the L1 norm of key scale wavelet detail component is calculated, macro fluctuation and surface texture energy coefficient are separated, and the quantitative representation of wall surface macro flatness deviation is realized.