The invention discloses a preparation method and application of a dual-channel multifunctional multicolor fluorescent probe, and relates to the technical field of fluorescent sensing detection.The dual-channel multifunctional multicolor fluorescent probe is prepared through the following steps that
pyromellitic acid and ZrCl4 solids are dispersed in deionized water, Zr-MOF is prepared through a hydrothermal method, pamoic acid and
melamine are dispersed in deionized water, and the Zr-MOF is prepared through a hydrothermal method; the preparation method comprises the following steps: preparing green fluorescent carbon dots (CDs) by using a hydrothermal method, dispersing the CDs and Zr-MOF in deionized water according to a certain proportion, reacting, collecting a brown
solid, washing,
drying, dispersing the
solid in
ethanol, adjusting the pH to be alkaline, adding Eu (NO3) 3.6 H2O, reacting, filtering, and
drying in vacuum to obtain a target probe. The probe can accurately distinguish Cu < 2 + > ions of
tetracycline antibiotics (TCs) through two independent fluorescent channels, not only displays completely independent
spectral response, but also has the advantages of self-calibration, high sensitivity,
interference resistance, field
visual detection and the like. The multifunctional fluorescent probe has important significance for improving detection efficiency, reducing cost and
energy consumption, optimizing
resource utilization and promoting green low-carbon economy.