732 results about "Organophosphorus pesticides" patented technology

The invention discloses a Pseudomonas stutzeri JSD-008 and degradation for organophosphorus pesticide, which is preserved in the 'Chinese germ management committee center (CGMCC)' with preservation number at CGMCC No.1738, wherein the strain is separated from the soil at pollution discharge pore of pesticide processing plant polluted by organophosphorus pesticide; the thallus and ferment liquid with the thallus can be degradation agent of organophosphorus pesticide to degrade the chlorpyrifos into trichlopyridinenol and methyl parathion into nitrophenol; fitting for rapid in-situ rehabilitation polluted by field soil pesticide.

The invention provides a phosphorous removing agent for wastewater produced during producing organophosphorus pesticides and a preparation method thereof. The solid phosphorous removing agent which can effectively remove total phosphorus from the wastewater produced during producing the organophosphorus pesticides is prepared by mixing a mineral with a metal compound and supporting the metal compound with the mineral. The phosphorous removing agent and the preparation method have the following beneficial effects: the production process is simple; the production cost is low; no harmful gas or sewage is generated in the production process; and the phosphorous removing agent can be widely used for removing phosphorous from the wastewater produced during producing various organophosphorus pesticides and has an obvious phosphorous removal effect.

The invention relates to a method for rapidly detecting the residuals of organophosphorus pesticides in vegetables by utilizing an Au nano-particle colorimetric method, which belongs to the technical field of analytical chemistry. The method provided by the invention comprises the following detection steps: preparation of Au nano-particles (AuNps); establishment of a method for detecting organophosphorus pesticides, actual sample detection and the like. The method provided by the invention can simply, rapidly and sensitively detect methamidophos in the organophosphorus pesticides according to the color change of an Au nano-particle system, has high sensitivity and provides convenience for further research, production, supervision and the like.

The invention discloses molecular imprinting polymer which belongs to the technical field of pesticide molecular imprinting and preparation method thereof. The molecular imprinting polymer is prepared by the following steps of: mixing a polymerization reaction monomer, a cross-linking agent, a hole inducing agent, an initiating agent and an imprinting molecule diethyl(3-methyluren)(phenyl)methyl phosphonate, carrying out in-situ polymerization reaction under the condition of thermal initiation and/or photo-initiation to obtain a polymer containing diethyl(3-methyluren)(phenyl)methyl phosphonate; washing the polymer till an imprinting molecule is not detected; and then washing by an organic solvent to neutrality and drying in vacuum to obtain the molecular imprinting polymer. The invention also discloses applications of the molecular imprinting polymer in aspects of separating, purifying and detecting organophosphorus pesticide in a sample. The applications of the polymer prevent the influence of target analyte template leakage on a following adsorption experiment, can simultaneously detect various target molecules and widen the detecting range of the target molecules.

The invention discloses a fluorescence hyperspectral technology-based pesticide residue detection device and method, and relates to the technical field of agricultural product detection. The fluorescence hyperspectral technology-based pesticide residue detection device mainly comprises an excitation light source module, an image acquisition module, a PC, an object stage and the like; during detection, a database is established in advance, related information of different vegetables, different pesticides and different pesticide concentrations is acquired and input into the database, and the PC is used for processing fluorescence hyperspectral image data of a detected object, calling a model in the database for matching calculation, and finally outputting a detection result. Compared with a conventional detecting instrument, the device has the characteristics that the database for storing related information is provided and excitation light source bands are adjustable, can be used for on-site real-time detection of types and residue concentration of common organophosphorus pesticides on the surfaces of leafy vegetables, and is high in detection accuracy, high in data processing speed and non-destructive and environmentally-friendly.

A sensor system detects organophosphorus pesticide residue by inducing the aggregation of gold nanoparticles. A method comprises aggregating gold nanoparticles by a reaction between an organophosphorus pesticide and imidazole or a green fluorescent protein (GFP), and detecting the organophosphorus pesticide based on a absorption spectral change resulting from the aggregation. The system for detecting pesticide residue is useful as a biosensor for analyzing pesticide residue in situ, because the optical change of the reagent by the presence of an organophosphorus pesticide is distinct, the detection speed is fast, and the range of detection limits is broad.

The invention relates to preparation and application of a magnetic ferroferric oxide nanoparticle modified carbon nanotube composite material. The preparation method includes: mixing a divalent iron salt and a trivalent iron salt, adding NH3.H2O, conducting water bath and centrifugal separation, washing the precipitate, and carrying out drying and grinding to obtain magnetic Fe3O4 nanoparticles; adding concentrated nitric acid and concentrated sulfuric acid into pretreated carbon nanotubes, conducting heating refluxing, carrying out filtering, deionized water washing and vacuum drying, thus obtaining purified multiwalled carbon nanotubes; adding the purified multiwalled carbon nanotubes into a triethylene glycol solution, conducting ultrasonic treatment, adding magnetic Fe3O4 nanoparticles, performing stirring and heating, conducting heat preservation and cooling, separating the product, and conducting vacuum drying, thus obtaining the magnetic Fe3O4 nanoparticle modified carbon nanotube composite material. The composite material is used for detecting the residue amount of organophosphorus pesticides in food. The preparation method utilizes magnetic Fe3O4 nanoparticles to modify carbon nanotubes, and greatly improves the dispersibility and adsorption properties of carbon nanotubes in water. The prepared magnetic Fe3O4 nanoparticle modified carbon nanotube composite material hasgood stability, and the maximum recovery rate can reach 89.6%.

The invention relates to a method for detecting an organophosphorus multi-pesticide residue in tea based on matrix effect compensation, which belongs to the technical field of safety detection of foods. The method comprises the following steps of: (1) mixing a pesticide standard solution; (2) preparing a sample solution; (3) detecting a sample to be detected by a gas chromatogram and mixing the standard solution. The method is rapid, simple and economic, has high sensitivity, effectively reduces or eliminates the interference of a complex matrix derived from the tea on the detection of organophosphorus pesticide residues and provides a scientific basis for further improving an evaluation system of the quality safety of the tea.

The invention belongs to the field of soil improvement, and specifically relates to a method for restoring organophosphorus pesticide contaminated soil. The method comprises the two phases of conditioning and stacking-decomposing, and chemical oxidation, wherein the phase of conditioning and stacking-decomposing comprises the following steps: 1) adding a strong-base medicament; 2) adding surfactant aqueous solution into soil prepared in the step 1); and 3) hermetically stacking the soil in the step 2) for 2-7 days; and the phase of chemical oxidation comprises the following steps: 4) adding a strong oxidant into the soil obtained in the phase of conditioning and stacking-decomposing; 5) preparing a strong acid into aqueous solution and adding the aqueous solution into the soil in the step 4), and rapidly stirring simultaneously to enable the soil to be weak-acidic finally; and 6) hermetically stacking the soil subjected to the adequate stirring treatment in the step 5), keeping the humidity of the soil and maintaining for 1-3 days. The restoration process of combining conditioning and stacking-decomposing with chemical oxidation, which is disclosed by the invention, is more stable and thorough compared with the method of restoring organic contaminated soil through simply and directly adopting chemical oxidation.

The invention discloses a rudimental immune antibody which is used to detect organic phosphorus pesticide residue and its application, the character of the invention is using diethyl phosphonic acid acetic acid as hapten, produce artificial immunogen through coupling with bovine serum albumin(BSA), and produce several clone antibody through immune Zelanian giant blanc or produce mono clone antibody through chmice immune, cell amalgamation, filtration, clone planting and other steps, the several and single antibody can be used to indirect emulative ELISA, direct ELISA, or immune test paper to detect the rudimental phosphorus pesticide. The excellences of the invention including: the selected hapten has several organic phosphorus pesticides general structure, the structure reforming is not necessary, it can immune animal after coupling with protein, it can also be used to detect organic phosphorus pesticide in food and water source, this show the rapid and convenient of the immune detecting tehchnology.

The invention relates to a preparation method of trichoderma multifunctional soil modifying agents. The preparation method comprises the steps that: trichoderma harzianum SH2303 with the CGMCC (China General Microbiological Culture Collection Center) NO. 4963 is cultured on a potato dextrose agar (PDA) culture medium for 2 to 3 days, a bacterial cake is punched by a hole puncher with the diameter being 5mm, the bacterial cake is inoculated into a seed culture medium for 5 to 7 days at the bacterium inoculation quantity of three tablets per bottle and is then inoculated into a spore production culture medium, the fermentation is carried out for 5 to 7 days under the optimized culture condition in a 300 L fermentation tank, and the number of thick-wall spores is (6 to 7)*10<8>/mL; and fermenting liquid and carriers are mixed according to the weight ratio of 1:1, the secondary open type solid fermentation is carried out for 5 to 7 days at the normal temperature, and the number of trichoderma conidiospores and chlamydospores is 200 to 500 million/g. The soil modifying agents prepared by the method have the main purposes of improving the primary and secondary salinization of vegetable planting soil and degrading the organic phosphorus pesticide residue soil, and have the effects of treating soil-borne diseases, promoting the crop growth and improving the crop yield. Because only one trichoderma strain is utilized, the fermentation process is easy to optimize, the microbial inoculum preparation cost is low, the commercial large-scale popularization is easy to realize, and high field general use values are reached.

The invention relates to a detection method of organophosphorus pesticide residues in tea. By using the surface enhanced Raman spectroscopy (SERS) as the detection method and using nano-gold as an SERS substrate, the tea is pretreated, and organophosphorus pesticides on the tea are enriched and separated so as to obtain a surface enhanced Raman spectrogram spectrogram by using a portable Raman spectrometer. Experiments prove that the detection method of the organophosphorus pesticide residues in the tea is easy to operate and high in accuracy, and can quickly detect organophosphorus in the tea, and false positive is avoided.

The invention discloses a method for detecting organophosphorus pesticide residual quantity, comprising the following steps of: (1) measuring a specimen spectrogram of a known organophosphorus pesticide; (2) measuring a spectrogram of an organophosphorus pesticide to be measured; (3) obtaining the category and the concentration of the organophosphorus pesticide through contrasting the spectrogram of the organophosphorus pesticide to be measured and the specimen spectrogram of the known organophosphorus pesticide. Meanwhile, the invention also discloses a liquid porphyrin optical detection apparatus for realizing the method. The detection method and the detection device have convenient and simple operation, simple and rapid detection, low cost, energy consumption and resource saving, and can qualitatively and quantitatively detect the category and the concentration of the organophosphorus pesticide residual quantity.

The invention discloses a PCN (Porous Coordination Network) 222 metal organic framework material as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing porphyrin, zirconium chloride and benzoic acid with N,N-dimethylformamide under the ultrasonic condition to obtain a mixed solution; carrying out hydrothermal reaction on the mixed solution, and carrying out ethanol washing, centrifuging and drying to obtain the PCN 222 metal organic framework material. The microtopography of the material is of a rod-like structure, and a crystal spindle is a hexahedron; the PCN 222 metal organic framework material has good thermal stability and can be used for enhancing the sensitiveness of a pesticide residue detection enzymatic inhibition method; the method disclosed by the invention has the advantages of simplicity, quickness and low cost and provides a practical tool for determining organophosphorus pesticide in a complex sample matrix.

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

The invention discloses a reagent kit and an enzyme-linked immunochromatography for detecting various organophosphorus pesticide residues and particularly relates to the reagent kit and the enzyme-linked immunochromatography for detecting organophosphorus pesticide residues in the production of vegetative agricultural products. The enzyme-linked immunochromatography for detecting the organophosphorus pesticide residues uses the high-sensitivity characteristic of enzyme to carry out detection by adopting the immunochromatography; compared with a traditional enzyme-linked immunoserbent assay (ELISA) for detecting organophosphorus pesticides, the enzyme-linked immunochromatography has the advantages of simplicity and convenience in operation, and visual result, and no special instrument equipment is needed; and compared with a colloidal gold immunochromatography, the enzyme-linked immunochromatography has the advantages of high sensitivity, strong specificity, accuracy in quantification by combining with a readout instrument and the like.

The invention discloses a method for detecting the organophosphorus pesticide residue in the tobacco, comprising the following steps of preprocessing by adding water of 5-15 times the weight of the tobacco sample to the tobacco sample to sock the tobacco sample, adding 10-20 ml of extracting agent to each gram of the tobacco sample to extract the tobacco sample, centrifuging to obtain organic-phase supernatant, purifying the organic-phase supernatant, concentrating the organic-phase supernatant by blowing with nitrogen, adding acetone solution to the organic-phase supernatant, and filtering the organic-phase supernatant with a organic-phase filtering membrane of 0.2 Mum to obtain the sample solution, wherein the extracting agent is the mixed solution of acetonitrile and acetone which are at the volume ratio of 1-2:1; and detecting the organophosphorus pesticide residue in the sample solution with gas chromatography and mass spectroscopy by using the chlorazol phosphorus as the internal standard substance after finishing preprocessing. Because the extracting agent is used for extracting in the preprocessing step, the target substance in the tobacco sample can be effectively extracted into the organic phase, and the precision for detecting the organophosphorus pesticide residue in the tobacco can be greatly improved.

The invention provides a superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA and a preparing method and application of the superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA. According to the superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA and the preparing method and application of the superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA, silylanization is conducted on the surface of superparamagnetic nanoparticle Fe3O4, a silylating reagent with a PSA structure is bonded, and thus a PSA group is introduced. The superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA and the preparing method of the superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA can be applied to the magnetic solid-phase extraction (M-SPE) field, and the superparamagnetic nanoparticle Fe3O4@SiO2@PSA modified by the PSA can serve as an adsorbent which is frequently used for magnetic solid-phase extraction, be applied to various pesticides such as carbamic acid ester, the organophosphorus pesticide and the herbicide and be used for absorption and extraction of target objects such as sulfonamides, organic acid and saccharides; meanwhile, the functional group of the PSA is a good binary ligand, in this way, the PSA is a good chelation material, can be used for extraction of metal ions and has high practical value and broad application prospect in the aspect of analytical investigation.

This invention discloses one immune glue gold test bar and process method for organophosphorus in agriculture test technique field, wherein, the test bar comprises PVC underlay, adhesive agent layer, sample pad, combination pad, aqua fortis fiber film, weight band, test band, compare band, overlap band, water absorptive pad, limit line and arrow line. The process method comprises the following steps: processing the medicine BSA load protein coupler; processing the medicine special signal clone antigen; processing sheep, rabbit or mouse antigen IgG; processing glue gold; processing single clone antigen gold label.

The invention discloses a chemiluminescence method for detecting an organophosphorus pesticide, and belongs to the field of pesticide residue analysis and the technical field of chemiluminescence analysis. The method comprises the following steps: 1, selecting a chemiluminescence system; 2, selecting a sensitizer; 3, performing single factor and orthogonal tests; 4, performing interference tests; 5, drawing a standard curve; 6, analyzing a sample; 7, researching a chemiluminescence mechanism; and 8, researching a relationship between a structure of the pesticide and chemiluminescence. The method has the advantages of simple and quick operation, wide linear range and low cost; an inorganic salt NaCl serves as the sensitizer of the system for the first time so as to improve the sensitivity of the system; and the method is suitable for detecting residue of organophosphorus pesticides with similar structures.

The invention relates to a method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms. Mainly composed of the processes of strain domestication, carrier preparation, bacterium adsorption and wastewater treatment, the method is characterized by: adsorbing domesticated fungus Aspergillus niger to activated carbon with a diameter of 5-8mml, then putting the prepared activated carbon containing saturated Aspergillus niger into wastewater, with the putting amount of activated carbon accounting for 1% of the wastewater when the CODcr (chemical oxygen demand) of each liter of sewage reaches 200-10000, controlling the temperature at 530DEG C, and performing static immersion for 1-6 days. By using the method to treat the organophosphorus pesticide wastewater, the degradation rate can reach up to 98%.

The invention discloses an antibody preparation method through which various organic phosphorus pesticide residues can be detected and the application, which belongs to the technical filed of agriculture. The antibody preparation method adopts the steps that firstly, O,O-diethylthiophosphoryl chloride is used to react with 4-aminobutyric acid to synthesize into general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP which comprises various organic phosphorus pesticide common structures-O, O-diethyl thiosulfate phosphoryl; secondly, the general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP is respectively coupled with bovine serum albumin BSA and chicken ovalbumin OVA by adopting carbodiimide method, to prepare general immunogen DENP-BSA and general coatingen DENP-OVA; and thirdly, multi-clonal antibody is prepared through immunized New Zealand white rabbits, the obtained antibody has obvious specificity to various organic phosphorus pesticides, such as thimet, demeton, disulfoion, omethoate, phoxim and quinalphos and can be used for the immune detection of the organophosphorus pesticides.

The invention provides a strain of degrading bacterium for effectively degrading organic phosphoric pesticide, the bacterium has a name of Ochrobactrum sp. and a docket number of CGMCC No.1221. The bacterium can be used for degrading organic phosphoric pesticide and nitrogen-containing aromatic hydrocarbons substance, thus used for the biological purification of water body, soil or agricultural products contaminated by these substances.

The invention relates to bacillus capable of efficiently degrading an organophosphorus pesticide and separated from rape phyllospheres, and provides a method for preparing a microbial inoculum thereof, which belong to the field of biotechnology. The bacillus is identified as bacillus subtilis, which is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, wherein the preservation No. is CGMCC No. 3374; the 16S rDNA gene sequence of the bacillus strain is submitted to a GenBank database; and the accession number is GU086422. The method for producing the microbial inoculum comprises the following 5 steps of: the preparation of a culture medium, the activation of a bacterium strain, the preparation of a liquid seed, the liquid state fermentation and the preparation of the microbial inoculum, wherein the fermentation culture medium takes molasses as a main raw material. Because the organophosphorus pesticide degrading microbial inoculum prepared by the technical scheme of the invention can efficiently degrade the organophosphorus pesticide residue on plant leaves in situ, the microbial inoculum can guarantee the safety of foods and protect the ecological environment.

The invention provides an analyzing method for simultaneously determining residues of organophosphorus pesticides and pyrethriods pesticides in soil. The analyzing method is characterized by comprising the following steps: carrying out ultrasonic extraction on a soil test sample by using an acetonitrile solution containing 1% of acetic acid by volume percent; purifying by using a suitable amount of PSA and C18 mixed fillers; blowing liquid supernatant by nitrogen and concentrating until the liquid supernatant is nearly dried; respectively dissolving by acetone and n-hexane and enabling the volume to be constant; raising the temperature by using DB-1701 and HP-5 quartz capillary columns and separating; carrying out GC/FPD (Gas Chromatograph/Flame Photometric Detector) detection and GC/micro ECD (Electron Capture Detector) detection; and finally, quantifying by using a base material external standard method. A test confirms that the method has a wide linear range; the technical indexes including detection sensitivity, accuracy and precision meet the requirements of a residue analysis; the pre-treatment operation is simple, convenient and rapid and a reliable manner is provided for analytical researches for simultaneously determining the residues of ths pesticides and the pyrethriods pesticides in the soil.e organophosphoru.

The invention discloses a carbon nanotube-based magnetic organophosphorus molecularly-imprinted polymer and its preparation method and use, and relates to a molecularly-imprinted polymer and its preparation method and use. The carbon nanotube-based magnetic organophosphorus molecularly-imprinted polymer and the preparation method solve the problem that the existing molecularly-imprinted polymers have poor dispersibility, the existing molecularly-imprinted polymer preparation method has poor applicability, and in use of the existing molecularly-imprinted polymers as adsorbents in sample pre-treatment in organophosphorus pesticide residue analysis, operation time is long and organic solvent consumption is large and selectivity is poor. The carbon nanotube-based magnetic organophosphorus molecularly-imprinted polymer comprises carbon nanotubes, FeCl3.6H2O, a cross-linking agent and a functional monomer. The preparation method comprises the following steps of 1, activating the carbon nanotubes, 2, adding FeCl3.6H2O into the activated carbon nanotubes, carrying out treatment and carrying out magnetic substance collection, 3, carrying out dispersion and adding tetraethyl orthosilicate into the dispersed system, 4, carrying out dispersion and carrying out magnetic substance collection, 5, preparing a magnetic molecularly-imprinted polymer having a template molecule, and 6, treating the magnetic molecularly-imprinted polymer to obtain the carbon nanotube-based magnetic organophosphorus molecularly-imprinted polymer. The carbon nanotube-based magnetic organophosphorus molecularly-imprinted polymer as an adsorbent can be used for selective extraction separation of organophosphorus pesticide residues in a complex sample.

The invention discloses a pre-treatment and analysis method of semi-volatile organic materials (64 types) and organophosphorus pesticides (9 types) in water. The pre-treatment and analysis method comprises the following steps of: in the pre-treatment process, adjusting the pH value of a water sample to 2-3 by using hydrochloric acid and sodium hydroxide; and then, utilizing an ENVI-ChromP solid-phase extraction column to carry out solid-phase extraction; utilizing dichloromethane-ethyl acetate with the volume ratio of 2:1 to elute the organic materials enriched on the solid-phase extraction column, modifying a solid-phase extraction device and combining the steps of eluting and dehydrating, so as to simplify the procedure; and carrying out detection analysis by using a gas chromatograph-mass spectrometer or a gas chromatograph-flame photometric detector. According to the method provided by the invention, dozens of types of substances are pre-treated simultaneously, so that a subsequent qualitative and quantitative detection is convenient to carry out, in this way, the method is particularly applicable to standardized analysis. The analysis method established by the invention has the advantages of low detection limit, small use amount of organic solvent, better recycling rate, high analysis efficiency and the like, thereby solving the problem that the existing method does not include the treatment and the detection of phenol and aniline substances at the same time.

The invention discloses a surface imprinted polymer for catalyzing degradation of an organophosphorus pesticide and a preparation method thereof and relates to the field of surface imprinted polymers. The preparation method comprises the following steps of: preparing polystyrene seeds by a soap-free emulsion method; preparing polystyrene microspheres with particle diameter of 2 to 6 microns by a fractional swelling method; and bonding a chain transfer agent on the surfaces of the polystyrene microspheres. The preparation method comprises the main steps of: performing chloride acetylation on the microspheres with chloroacetic chloride, then aminating with ethydene diamine and then bonding the chain transfer agent with an amino; adding a template molecule under a proper solvent and performing graft polymerization on an amino acid derivative functional monomer and a cross-linking agent on the surfaces of the microspheres to form the needed polymer carrier surface template imprinted microspheres. The surface imprinted polymer with catalyzing, degrading and purifying functions on an organophosphorus compound is synthesized by taking an organophosphorus compound catalyzing and degradingreaction substrate as the template and organically combining the high-crosslinking degree insoluable porous polymeric microspheres with the template imprinting technology through a surface controllable graft hydrogel.