Preparation of graphene/enzyme electrochemical biosensor and detection on organophosphorus pesticide residue

A biosensor and organophosphorus pesticide technology, applied in the field of graphene/enzyme electrochemical biosensor and its preparation, can solve the problems of graphene oxide instability, and achieve the effects of excellent stability, simple operation and fast detection

Inactive Publication Date: 2015-12-09
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, graphene oxide is not stable in neutral and alkaline solutions suitable for immobilizing AChE

Method used

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  • Preparation of graphene/enzyme electrochemical biosensor and detection on organophosphorus pesticide residue
  • Preparation of graphene/enzyme electrochemical biosensor and detection on organophosphorus pesticide residue
  • Preparation of graphene/enzyme electrochemical biosensor and detection on organophosphorus pesticide residue

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Embodiment Construction

[0030] In order to make the purpose, technical solution and advantages of the present invention clearer, the specific implementation manners of the present invention will be described in detail below. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] 1. Materials and equipment

[0032] 1.1 Materials

[0033] Table 1 Experimental Reagents

[0034]

[0035]

[0036] 1.2 Instruments

[0037] Table 2 Experimental Instruments

[0038]

[0039]

[0040] 2 Experimental methods

[0041] 2.1 Preparation of graphene oxide

[0042] GO was prepared by the improved Hummers method, and the specific steps are as follows:

[0043] (1) Pre-oxidation of graphene: add 1gK in a 250mL round botto...

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Abstract

The invention provides a method including the steps of preparing graphene oxide through improved hummers method, modifying a graphene oxide (GO) / chitosan (CS) mixed solution onto surface of a glass carbon electrode (GCE), modifying acetylcholin esterase (AChE) onto the surface of the GCE having a GO-CS mixture film to form a graphene oxide-chitosan-acetylcholin esterase / glass carbon electrode (GO-CS-AChE / GCE), thereby constructing a graphene / enzyme electrochemical biosensor for detecting organophosphorus pesticides. The graphene / enzyme electrochemical biosensor is used for detecting carbaryl and trichlorphon, of which linear ranges are respectively 10-100 nmol / L and 10-60 nmol / L and correlation coefficients are respectively 0.993 and 0.994. Calculation proves that detection limits of the both pesticides are 3.3 nmol / L (wherein the detection limit is 1 / 3 of a minimum detection concentration). The graphene / enzyme electrochemical biosensor is high in sensitivity, is simple in preparation and is convenient to operate. The electrochemical biosensor can be applied in detection of organophosphorus pesticides and has potential in detection of the organophosphorus pesticides.

Description

technical field [0001] The invention relates to a graphene / enzyme electrochemical biosensor and a preparation method thereof. The electrochemical biosensor can be applied to the detection of organophosphorus pesticides and has the advantages of high sensitivity, simple preparation, convenient operation and the like. Background technique [0002] Organophosphorus pesticides (organophosphorus pesticides, OPs) are a class of high-efficiency, broad-spectrum insecticides and herbicides, and are widely used in agriculture because of their high insecticidal activity. However, the improper use of organophosphorus pesticides leads to a large amount of residues, which poses a huge threat to the environment and human health. Organophosphorus pesticide poisoning is mainly due to the fact that OPs can inhibit the activity of acetylcholinesterase (AChE), causing excessive accumulation of acetylcholine (ATCl), causing central nervous system regulation dysfunction, leading to respiratory sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 李承勇莫日坚聂芳红
Owner GUANGDONG OCEAN UNIVERSITY
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