176 results about "Methamidophos" patented technology

The invention relates to a method for rapidly detecting residual organophosphorus pesticide in vegetables by utilizing a double-signal method of CdTe quantum dots and gold nanoparticles (AuNPs), and belongs to the technical field of analytical chemistry. The technical scheme provided by the invention comprises the following detecting steps: synthesizing and purifying a CdTe quantum dot aqueous solution; preparing AuNPs; performing TEM (transmission electron microscope) characterization on AuNPs and a AuNPs-substrate-enzyme system respectively; studying a fluorescence spectrum and an absorption spectrum which are interactive between CdTe quantum dots and AuPNs; and utilizing the double-signal method of CdTe quantum dots and AuNPs to rapidly detect the organophosphorus pesticide. The method can detect methamidophos in the organophosphorus pesticide simply, rapidly and sensitively, has very high sensitivity and provides convenience for subsequent research, production, supervision and the like.

The present invention discloses a pesticide main agent for controlling poplar longicorn and preparation made up by using said pesticide main agent. Said main agent is made up by mixing 2.0-20.0% of acetamiprid and 10.0-40.0% of dichlorvos or trichlorfon or acephate.

The invention belongs to the technical field of pesticide residue determination, relates to a vegetable and fruit pesticide residue pre-treatment method and in particular relates to an extraction and purification method for determining organophosphorus pesticide residues in fruits and vegetables. The extraction and purification method comprises the following steps of: sampling, adding an acetonitrile extracting solution, purifying by virtue of a MycoSep 226 AflaZon+ small column, and then determining. According to the extraction and purification method, dosage of an organic reagent is reduced, and nitrogen blowing or rotary steaming equipment is not required; a step of detecting organic phosphorus in a conventional method is not required, manpower is reduced, and detection efficiency is improved; purification degree of an organic phosphorus extracting solution is obviously improved, the obtained sample injection solution is clear and transparent, no obvious pollution on a detection system is produced after sample injection is carried out for multiple times, sensitivities of instrument and equipment are consistent, and a detection limit is constant; and peak height is not reduced and peak shape is constant after the sample injection is carried out for multiple times even if residues of organophosphorus pesticides, such as methamidophos, acephate, moncrotophos and omethoate with strong polarity, are determined. The extraction and purification method for detecting organophosphorus pesticide residues in fruits and vegetables has a good application prospect in the technical field of pesticide residue determination.

The invention relates to a molecular imprinting fluorescent microsphere array detection card and application thereof. A fluorescent molecular imprinting microsphere array is adhered and fixed in a hard card to obtain the molecular imprinting fluorescent microsphere array detection card. The molecular imprinting fluorescent microsphere array detection card disclosed by the invention is high in recognition ability, high in adsorbing capacity, short in adsorption time, high in fluorescence intensity and stable, and the defects that the pretreatment is complicated and the sensitivity is low during the traditional acephate detection are overcome; ordered arrangement of the microsphere array is favorable for full contact between imprinting microspheres and targets, the defects that the microspheres are settled in a solution and the fluorescence intensity is influenced are overcome, an accurate, sensitive and rapid novel method is provided for detecting acephate pesticide residues, and a certain reference is provided for developing a detection technology for other types of pesticide residues.

The invention relates to a water emulsion used for controlling cnaphalocrocis medinalis, and pertains to the technical field of pesticides. The water emulsion is composed of the following components by weight percentage: 10 to 40 percent of chlorpyrifos, 0.2 to 5 percent of emamectin benzoate, 5 to 15 percent of emulsity agent, 1 to 10 percent of antifreezing agent, 10 to 30 percent of solvent and water for the rest supplement. Compared with the existing agents for controlling cnaphalocrocis medinalis, the water emulsion has the following advantages that: 1. as alkylbenzene sulfonate metal salt is taken as the emulsity agent, the decomposition of the emamectin benzoate in the water can be retarded and the pesticide application effect is improved; 2. the water emulsion has obvious synergy to the rice fulgorid; 3. the water emulsion can replace riskiest pesticides (acephatemet and the like), reduce the dosage and the usage amount of the organic solvent, and reduce the environment pollution and the farm product remain; 4. the water emulsion can retard the drug resistance of the cnaphalocrocis medinalis and improve the desinsection speed.

The invention discloses an aptamer Met-G02 specifically combined with methamidophos. The aptamer Met-G02 is screened by an SELEX technology. The aptamer is single stranded DNA and is prepared from 82nucleotides, a nucleotide sequence of the aptamer is shown as SEQ ID NO:1, and a secondary structure of the aptamer contains a protruded ring and a stem; furthermore, the aptamer has a G-quadruplex structure, and the Met-G02 gibbs free energy DG is equal to -15.21. A series of character assessments on aptamer specificity, affinity, flexibility and the like are performed based on enzyme linked oligonucleotide absorption measurement, and a dissociation constant Kd of the Met-G02 is shown to be equal to 11.77+ / -2.07 nM; furthermore, the aptamer can be specifically combined with the methamidophos;thus, the aptamer has the advantages of high specificity, high affinity and quick and flexible application to methamidophos detection.