15 results about "Methamidophos" patented technology

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

This invention belongs to the field of agricultural fungicides, and provides a fungicidal composition containing methamidophos and fluopyram and its application, wherein the mass ratio of methamidophos and fluopyram is 1:2-1:70; the fungicidal composition has excellent synergistic effects on crop diseases; it can effectively reduce the dosage of pesticides, slow down the development of pesticide resistance in diseases, reduce environmental pollution, and is safe for crops in field observations.

The present invention belongs to the technical field of pesticides, and specifically relates to an insecticidal composition containing 4-(trifluoromethyl)nicotinamide, and an application thereof. The insecticidal composition includes an active ingredient A and an active ingredient B, wherein the active ingredient A is 4-(trifluoromethyl)nicotinamide, and the active ingredient B is selected from one or more of the following compounds: afidopyropen, formula (I), dimpropyridaz, formula (II), isoflualanam, fipronil, imidacloprid, thiamethoxam, avermectin, acephate and beta-cypermethrin. The composition has features such as reasonable components, good insecticidal effect, low administration cost, reduced application amount, crop safety, and reduced residue.

The invention relates to a method for detecting pesticide based on a ferriporphyrin modified covalent organic framework nano-enzyme sensing array. According to the invention, ferriporphyrin molecules are loaded on a porous and ordered COFs skeleton, so that excellent peroxidase simulated catalytic activity is realized, H2O2 can be catalyzed to be decomposed to generate reactive oxygen free radicals, and TMB, OPD and ABTS chromogenic reactions are catalyzed. Based on the performance, a three-channel colorimetric sensing array is constructed to detect and distinguish various pesticides such as malathion, profenofos, chlorpyrifos, acephate and dimethoate, and the detection limit is as low as 0.341 nM. After different pesticides are adsorbed to active sites, catalytic reaction can be remarkably inhibited, and specific colorimetric fingerprint signals are generated. Colorimetric data is further combined with a machine learning algorithm, high-accuracy classification and recognition of single pesticides and mixtures thereof are achieved by means of a multilayer sensor, a support vector machine, a random forest and other models, the classification accuracy of an MLP model reaches 90%, ROC curve AUC exceeds 0.97, and the reliability of the detection method is proved.

This invention discloses Ti3C2T x This research focuses on the aerogel growth of gold nanomaterials using (MXene) / functionalized graphene quantum dots and their applications, belonging to the field of organophosphorus pesticide analysis and detection technology. Citric acid, serine, and arginine were dissolved in deionized water and heated to obtain citrate-arginine-serine quantum dots; Ti3A1C2(MAX) was then etched using lithium fluoride and hydrochloric acid to obtain Ti3C2T. x (MXene); To Ti3C2T x A quantum dot solution was added dropwise to generate a microgel; a gold nanoparticle growth solution was added dropwise to the microgel, followed by incubation with chloroauric acid and then a reducing agent. After the reaction was complete, the mixture was centrifuged, washed, and vacuum dried to obtain graphene quantum dot-functionalized gold nanoparticles. This invention utilizes the superior conductivity of Ti3C2T... x Gold nanoparticles were grown on functionalized graphene quantum dot microgels and used as electrocatalytic active materials. An electrochemical sensor was constructed using aptamers as recognition molecules to detect the organophosphorus pesticide methamidophos.

This invention belongs to the field of agricultural fungicides, and provides a fungicidal composition comprising methamidophos and fluthiazopyrone and its application. The active ingredients are composed of methamidophos and fluthiazopyrone, with a mass ratio of methamidophos to fluthiazopyrone of 1:1 to 1:20. The fungicidal composition has excellent synergistic effects against crop diseases, can effectively reduce the amount of pesticides used, slow down the development of pesticide resistance in diseases, reduce environmental pollution, and is safe for crops in field observations.

The present invention provides compositions comprising phosphoramidothioates that are resistant to degradation of active ingredient. The invention provides a process for preparing granular compositions comprising acephate that are resistant to degradation of active ingredient, wherein said process comprises use of a mixture of water and an electrolyte.

The present invention relates to a use of Pseudomonas knackmussii WHP-AP2. The Pseudomonas knackmussii WHP-AP2 has a deposit number of GDMCC NO: 64331. The Pseudomonas knackmussii WHP-AP2 of the present invention can quickly and efficiently degrade acephate at a degradation rate of 96% or more, can degrade acephate having a wide range of pH values, is resistant to high-concentration acephate, and has good application prospects in the control of environmental pollution in acephate soil, water sources, etc.

The invention relates to a preparation method and application of acephate hapten, complete antigen, fluorescent marker and antibody. The acephate hapten has a structure as shown in a formula I or a formula II, and a specific active connecting arm is introduced into an acephate molecule, so that after the acephate molecule is coupled with a carrier protein, a characteristic structure fragment of acephate can be fully exposed, and a complete antigen with good immunogenicity is constructed. The antigen can effectively stimulate the immune system of a host animal to generate an anti-acephate antibody with high specificity and high sensitivity. The antibody prepared by the invention has high affinity and specificity to acephate, and is suitable for high-throughput immunoassay detection of acephate residues in a complex matrix. An enzyme linked immunosorbent assay kit researched and developed based on the antibody is easy and convenient to operate and high in sensitivity, has good practicability and industrialization prospects, and can provide reliable technical support for agricultural product quality safety monitoring and environmental pollution control.

The present invention provides compositions comprising phosphoramidothioates that are resistant to degradation of the active ingredient. The invention provides a process for preparing granular compositions comprising acephate that are resistant to degradation of the active ingredient, wherein said process comprises the use of a mixture of water and an electrolyte.

The invention discloses a meta-dephosphorized hapten, a complete antigen, an antibody and a preparation method and application thereof. The meta-dephosphorized hapten has the following structural formula. The metathion hapten is designed for metathion and can be used for preparing the metathion antibody, and the metathion antibody prepared through animal immunization and experimental verification can specifically recognize metathion. The hapten prepared by the invention and an immunoassay method established on the basis of the hapten are wide in linear range and relatively high in sensitivity, and can meet the requirement of rapid screening.

The application discloses a mevinphos hapten, a complete antigen, an antibody and a preparation method and application thereof, and the mevinphos hapten has the following structural formula: The mevinphos hapten is designed for mevinphos and can be used for preparing a mevinphos antibody; the mevinphos antibody prepared through animal immunization and experiment verification can specifically recognize mevinphos. The prepared hapten and the immune analysis method established based on the hapten have a wide linear range and high sensitivity, and can meet the requirement of rapid screening.

The present invention provides compositions comprising phosphoramidothioates that are resistant to degradation of active ingredient. The invention provides a process for preparing granular compositions comprising acephate that are resistant to degradation of active ingredient, wherein said process comprises use of a mixture of water and an electrolyte.

The invention belongs to the field of organic synthesis and pesticide application, and particularly relates to application of a novel isoindolone substance, the structural formula is as follows: R1 is hydrogen, 3-methyl, 4-methyl, 4-methoxyl, 3-methoxyl or 2-methoxyl chloride; meanwhile, the N-methylbenzylamine can also be changed into N-methyl-N-(4-picolyl) amine (2) and N-methyl-2-thiophenemethylamine (3). Wherein R2 is selected from hydrogen, 3-methyl, 4-methyl, 5-methyl, 6-methyl, 3-methoxyl, 4-methoxyl, 5-methoxyl, 6-methoxyl, fluorine and chlorine; meanwhile, the 2-methoxy methyl benzoate can also be changed into the 2-methoxy methyl nicotinate pyridine (4). A biological activity determination result shows that the isoindolone compound containing the thiophene and methyl structure has medium to excellent inhibitory activity on phytophthora capsici, the activity of the compound A31 is the best, the inhibitory activity on the phytophthora capsici is 95 + / -3% and is equivalent to that of a control drug metalaxyl, and EC50 is equal to 1.3048 mu g / mL and is superior to that of the control drug metalaxyl.

The invention discloses a composition containing emamectin benzoate and spinetoram, a preparation and application of the composition and the preparation. The microemulsion comprises an active component, a conventional auxiliary agent and a key functional systemic accelerator. An organic silicon surfactant (such as Silwet L-77) and methylated vegetable oil (such as Pro-MSO) are compounded according to a specific proportion to serve as a functional uptake accelerant, the combination can generate a synergistic effect, and uptake conductivity of active ingredients in plants is remarkably improved. Indoor biological assay results show that the LC50 value of the preparation is reduced by more than 50% compared with that of a traditional formula; field pharmacodynamic tests prove that under the condition of the same dosage of the effective components, the control effect on the eggplant thrips reaches up to 95% or above, and the persistence is excellent. According to the invention, through innovative optimization of an auxiliary system, synchronous improvement of the pesticide utilization rate and the control effect is realized, and an effective scheme is provided for developing a novel pesticide preparation which is efficient, low in dosage and capable of delaying resistance.