A dual synergistic compound insecticide composition and application

Abstract

The present invention belongs to the field of pesticides and relates to a binary synergistic compound insecticide composition and application. The composition is composed of two active ingredients, compound A and compound B, and the component B is selected from any one or two of organophosphorus insecticides and acaricides; the organophosphorus insecticides include chlorpyrifos, triazophos, acephate, phoxim, diazinon, malathion, etc.; the compound I-72 has the following structure: the composition of the present invention has the advantages of obvious synergy and delayed resistance, and can be used to prevent and control various pests such as Lepidoptera, Thysanoptera, Diptera, Hemiptera, and has a good control effect on harmful mites and nematodes.

Description

Technical Field

[0001] The invention belongs to the field of pesticides and relates to a binary synergistic compound insecticide composition and application. Background Art

[0002] In the actual process of agricultural production, the application of chemical agents is one of the most effective means of preventing and controlling plant diseases and insect pests. However, the most common problem caused by chemical pest control is insect resistance. At the same time, the long-term continuous high-dose application of a single chemical agent is likely to cause a series of problems such as agent residues and environmental pollution. Reasonable chemical agent compounding or mixing has the positive characteristics of expanding the control spectrum, improving the control effect, extending the appropriate period of application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence and development of pest resistance and drug resistance. Chemical agent compounding or mixing is one of the most effective ways to solve the above problems.

[0003] Chemical pesticides are an important guarantee for stable and abundant crop yields, and they play an irreplaceable role in pest control. Organophosphorus insecticides are highly toxic to pests (including mites), and most varieties have high efficacy and are used at low concentrations. Generally, they are more effective when the temperature is high. Their insecticidal mechanism is to inhibit the activity of cholinesterase and poison the pests. Most organophosphorus insecticides have broad-spectrum insecticidal effects, killing mosquitoes, flies, ticks, mites, lice, nematodes, bedbugs, etc., and have different insecticidal effects such as contact killing, stomach poisoning and fumigation; they have high efficiency and rapid killing performance, and produce less resistance or cross-resistance.

[0004] Although organophosphorus pesticides have been used for a long time and their efficacy has decreased compared to the past, pests have developed resistance to them relatively slowly, and they are still widely used. At the same time, they are generally safe for crops and are not prone to causing pesticide damage.

[0005] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 is a new type of insecticide created by Shenyang Sinochem Pesticide Chemical Research and Development Co., Ltd., which has contact activity, has outstanding control effects on pests, and has a unique mechanism of action and no cross-resistance with existing insecticides. The structural formula of the compound is as follows:

[0006]

[0007] In order to extend the service life of new varieties, compounding two ingredients with different mechanisms of action may produce some unexpected technical effects, and is also one of the effective methods for preventing and controlling resistant pests and improving drug efficacy. However, there has been no report on the compound I-72 and organophosphorus insecticide combination composition so far. Summary of the invention

[0008] The purpose of the present invention is to provide a binary synergistic compound insecticide composition and application.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is:

[0010] A binary synergistic compound insecticide composition, the composition is composed of two active ingredients A and B, wherein the active component A is selected from a compound having the following structure (i.e., compound I-72), and the active component B is selected from an organophosphorus insecticide and acaricide; the weight ratio of the two active components is 100:1 to 1:100;

[0011] The compound has the following structure:

[0012]

[0013] The organophosphorus insecticide is selected from acephate, chlorpyrifos, chlorpyrifos-methyl, omethoate, diazinon, methamidophos, monocrotophos, parathion-methyl, parathion, terbufos, phoxim, methidathion, triazophos, azinphos-methyl, inphos-methyl), azinphos-ethyl, chlorethoxyfos, chlorfenvinphos, chlormephos, coumaphos, cyanophos, phosmet, dichlorvos, dicrotophos, dimethylvinphos, disulfoton, EPN, ethion, fenthion, heptene heptenophos, mecarbam, mevinphos, naled, oxydemeton-methyl, phenthoate, isofenphos, pirimiphos-methyl, tebupirimfos, propaphos, propetamphos, prothiofos, pyraclofos, pyridaphenthion, quinalphos quinalphos), sulfotep, temephos, tetrachlorvinphos, thiometon, trichlorfon, acethion, malathion, phosalone, vamidothion, naftalofos, isoxathion, pyrazophos, fenitrothion, sulprofos, nitrate,azamethiphos, diazinon, fonofos, bromophos-ethyl, bromfenvinfos, trithion, cyanophenphos, demeton methyl, dioxabenzofos, flupyrazofos, formothion, fosmethilan, lodofenphos, iprobenfos, methacrifos, phoemet, pyridathion, prothoate, tebupirimfos;

[0014] The active component A in the composition is selected from the compound with the above structure, and the active component B is selected from chlorpyrifos, triazophos, acephate, phoxim, diazinon and malathion; the weight ratio of the two active components is 50:1 to 1:50.

[0015] The active component A in the compound is selected from the compound with the above structure, and the active component B is selected from chlorpyrifos, triazophos, acephate, phoxim, diazinon and malathion; the weight ratio of the two active components is 20:1 to 1:20.

[0016] The active component A in the composition is selected from the compound with the above structure, and the active component B is selected from chlorpyrifos, triazophos, acephate, phoxim, diazinon and malathion; the weight ratio of the two active components is 10:1 to 1:10.

[0017] The binary insecticide composition can be used to control pests, mites and nematodes in agriculture, forestry, fruit, vegetable, tea and the like.

[0018] The insecticide composition of the present invention has a significant synergistic effect in controlling pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops or urban health pests. Indoor biological activity test results show that mixing compound I-72 with organophosphorus insecticides can improve the control effect, reduce the dosage, and expand the insecticide spectrum. For example, when compound I-72 is mixed with chlorpyrifos, triazophos, malathion, etc. in a ratio of 1:10 to 10:1, the activity against western flower thrips can be greatly improved; when compound I-72 is mixed with chlorpyrifos and acephate in a ratio of 10:1 to 1:10, it has a synergistic effect on the control of diamondback moth, corn borer, etc.; when compound I-72 is mixed with chlorpyrifos, triazophos, phoxim in a ratio of 10:1 to 1:10, it has a synergistic effect on the control of American white moth; when compound I-72 is mixed with chlorpyrifos, diazinon, acephate, etc. in a ratio of 10:1 to 1:10, it has a synergistic effect on the control of American white moth; When compound I-72 is mixed with chlorpyrifos, diazinon and phoxim in an appropriate ratio, it has a synergistic effect on the control of cotton aphids; when compound I-72 is mixed with chlorpyrifos and acephate in a ratio of 10:1 to 1:10, it has a synergistic effect on the control of whiteflies; when compound I-72 is mixed with chlorpyrifos, acephate, etc. in a ratio of 1:10 to 10:1, it shows a good synergistic effect on yellow flea beetles; through a large number of experiments, it was found that after compound I-72 was mixed with organophosphorus insecticides, there was a significant synergistic trend with the increase of the amount of the compound, which is conducive to the application of the compound in the field. Therefore, another technical solution of the present invention is the use of the two active component compositions of the present invention for preparing insecticides and acaricides.

[0019] The insecticide composition of the present invention can be used to control pests, mites, underground pests, nematodes or urban health pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, etc., and has a protective effect on various plant diseases; the pests include all insects of Lepidoptera, Thysanoptera, Coleoptera, Hemiptera, Diptera, Homoptera, Orthoptera, Isoptera, Hymenoptera, as well as mites, nematodes or parasitic pests. It can be particularly used to control Lepidoptera pests such as Chilo suppressalis, Corn borer, Diamondback moth, Armyworm, Leaf miner, Cotton bollworm, Beet armyworm, termite, urban health pests and piercing-sucking mouthparts pests such as planthoppers, whiteflies, aphids, thrips, leafhoppers, stink bugs, flea beetles on various crops.

[0020] In particular, the insecticidal and acaricidal composition of the present invention can be particularly used for controlling Lepidoptera. A non-exhaustive list of these pests includes, but is not limited to: Longwinged troller, Brownbanded troller, Clearwing moth, Spodoptera, Cotton leafworm, Amylois spp., Bean borer, Yellow troller, Argyresthia spp., Banded troller, Spodoptera, Cotton miner, Corn armyworm, Cabbage moth, Peach moth, Graminus spp., Leaf roller, Chrysote uchia topiaria, Gramineus moth, Leaf roller, Cloud troll, Leaf roller, Cosmophila, Pieris, Cosmophila flava, Grass moth, Cabbage moth, Apple troll, Boxwood moth, Troll, Boxwood moth, Pyralidae, Pyralidae, Cotton bollworm, Diamond borer, African stem borer, Powderyfly, Epinotia spp), Etiella zinckinella, Etiella spp, Etiella spp, Etiella spp, Etiella spp, Etiella spp, Feltia jaculiferia, Grapholita spp, Etiella ... gossypi-ela, coffee leafminer, armyworm, potato moth, cabbage butterfly, Pieris spp., diamondback moth, bud moth spp., leaf moth spp., mint armyworm, Richia albicosta, Scirpophaga spp., stem borer spp., palpalized moth spp., Spodoptera spp., cotton leaf folder, Xingpengwei spp., Heteropoda spp., leaf folder spp., cabbage looper, tomato leafminer, and nest moth spp.

[0021] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling Thysanoptera. A non-exhaustive list of these pests includes, but is not limited to: Frankliniella spp., Heliothrips spp., Hercinothrips spp., Parthenothrips spp., Scirtothrips aurantii, Sericothrips variabilis, Taeniothrips spp., Thrips spp.;

[0022] In particular, the insecticidal and acaricidal compositions of the present invention are particularly useful for controlling Hemiptera. A non-exhaustive list of these pests includes, but is not limited to, Acrosternum hilare, green stink bug, Blissus leucopterus, chinch bug, Calocoris norvegicus, Cimex hemipterus, tropical bed bug, Cimex lectularius, bed bug, Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus, cotton bug, Edessa meditabunda, Eurygaster maura, cereal bug, Euschistus heros, Euschistus servus), brown toon), Helopeltis antonii, Helopeltis theivora, tea horn stink bug, Lagynotomus spp., stink bugs, Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp., Lygus, Lygus hesperus, western grass stink bug, Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula, southern green stink bug, Phytocoris spp., Lygus, California plant stink bug californicus), Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus, Four-lined stink bug, Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp., bloodsucking conenose bugs / kissing bugs.

[0023] In particular, the insecticidal and acaricidal composition of the present invention can be particularly used for controlling Homoptera (aphids, scale insects, whiteflies, leafhoppers). A non-exhaustive list of these pests includes, but is not limited to: Acrythosiphon pisum, pea aphid, Adelges spp., aphid, Aleurodes proletella, cabbage whitefly, Aleurodicus disperses, Aleurothrixus floccosus, whitefly, Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp., leafhopper, Aonidiella aurantii, Aonidiella aurantii, Aphis spp., aphid, Aphis gossypii, cotton aphid, Aphis pomi, apple aphid, Aulacorthum solani, foxglove aphid, Bemisia spp.), whitefly, Bemisia argentifolii, Bemisia tabaci, sweet potato whitefly, Brachycolus noxius, Russian aphid, Brachycorynella asparagi, asparagus aphid, Brevennia rehi, Brevicoryne brassicae, cabbage aphid, Ceroplastes spp., scale insects, Ceroplastes rubens, red wax scale insects, Chionaspis spp., scale insects, Chrysomphalus spp., scale insects, Coccus spp., scale insects, Dysaphis plantaginea), pink apple aphid, Empoasca spp., leafhoppers, Eriosoma lanigerum, woolly apple aphid, Icerya purchasi, woolly scale, Idioscopus nitidulus, mango leafhopper, Laodelphax striatellus, brown planthopper, Lepidosaphes spp., Macrosiphum spp.), potato aphid (Macrosiphum euphorbiae), potato aphid, wheat aphid (Macrosiphum granarium), English wheat aphid, rose aphid (Macrosiphum rosae), rose aphid, aster leafhopper (Macrosteles quadrilineatus), aster leafhopper, raspberry foamhopper (Mahanarva frimbiolata), wheat aphid (Metopolophium dirhodum), rose wheat aphid, long-horned bug (Mictis longicornis), peach aphid (Myzus persicae), green peach aphid, black-tailed leafhopper species (Nephotettix spp.), leafhopper, black-tailed leafhopper (Nephotettix cinctipes), green leafhopper, green leafhopper, rice brown planthopper (Nilaparvata lugens), brown planthopper, bran shield scale (Parlatoria pergandii), bran scale, black scale scale (Parlatoria ziziphi), ebony scale, corn planthopper (Peregrinus maidis), corn flower-winged planthopper, Philaenus spp., Philaenus spp., Phylloxera vitifoliae, Phylloxera vitifoliae, Physokermes piceae, spruce round scale, Planococcus spp., mealybugs, mealybugs (Pseudococcus spp.), mealybugs, pineapple mealybugs (Pseudococcus brevipes), pineapple mealybugs, Quadraspidiotusperniciosus, St. Joseph's scale, Rhapalosiphum spp.), aphids, Rhapalosiphummaida, corn aphids, Rhapalosiphum padi, Grain aphids, Saissetia spp., scale insects, Saissetia oleae, black scale insects, Schizaphis graminum, green aphids, Sitobion avenae, English wheat aphids, Sogatella furcifera, white-backed planthoppers, Therioaphis spp., aphids, Toumeyella spp., scale insects, Toxoptera spp., aphids, Trialeurodes spp.), whitefly, Trialeurodes vaporariorum, greenhouse whitefly, Trialeurodes abutiloneus, winged whitefly, Unaspis spp., scale insects, Unaspis yanonensis, arrow scale insects, and Zulia entreriana.

[0024] Advantages of the present invention: The binary composition obtained by compounding compound I-72 with organophosphorus insecticides, acaricides and nematicides in an appropriate ratio has a good synergistic effect, thereby improving the control effect, reducing the dosage and expanding the insecticide spectrum. Specifically:

[0025] 1. The two active components A and B in the compound have different mechanisms of action, which can delay the development of resistance of pests and mites. 2. The insecticide spectrum has been expanded, and it can be used to control a variety of pests, mites, and nematodes on fruit trees, vegetables, ornamental plants, tea, cotton and other crops; 3. The amount of pesticides used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within an appropriate ratio range, which improves the control effect of pests, mites, and nematodes such as the striped stem borer, rice brown planthopper, whitefly, armyworm, peach aphid, thrips, etc. DETAILED DESCRIPTION

[0026] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products. The active components in the formula examples are metered and added after 100%, and each component is measured by weight percentage.

[0027] Indoor Activity Assay Example

[0028] Example 1: Indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against western flower thrips

[0029] Test target: Western flower thrips (Frankliniella occidentalis Pergande), a sensitive strain reared indoors.

[0030] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0031] Test method: First, select kidney bean leaves and immerse them in the prepared liquid medicine in the order from low dose to high dose according to the experimental design for 10 s. After natural air drying, place them in a petri dish with a diameter of 9 cm containing filter paper, and introduce neat and healthy test insects. There are about 20 insects in each treatment, and each treatment is repeated 3 times. In addition, a blank control is set up.

[0032] Place the treated test materials in the observation room, where the temperature, humidity, and light in the observation room can be adjusted as needed. After 48 hours, investigate the number of dead and live insects, calculate the corrected mortality rate using the Abbott formula, and perform statistical analysis using the DPS data processing software to obtain the virulence regression equations, LC 50 values and 95% confidence limits of each tested single agent and each different ratio mixture. Then, use the Sun Yunpei (Sun y-p) method to calculate the co-toxicity coefficient of each ratio and evaluate the mixing effect. The test results are shown in Table 1.

[0033] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0034]

[0035] In the formula: ATI - the actual virulence index of the mixture; S - the LC 50 of the standard agent; M - the LC 50 of the mixture.

[0036] TTI = TIA × PA + TIB × PB

[0037] In the formula: TTI - the theoretical virulence index of the mixture; TIA - the virulence index of agent A; PA - the percentage content of agent A in the mixture; TIB - the virulence index of agent B; PB - the percentage content of agent B in the mixture.

[0038]

[0039] In the formula: CTC - co-toxicity coefficient; ATI - the actual virulence index of the mixture; TTI - the theoretical virulence index of the mixture.

[0040] The co-toxicity coefficient (CTC) of the compound mixture: CTC ≥ 120 indicates a synergistic effect; 80 < CTC < 120 indicates an additive effect; CTC ≤ 80 indicates an antagonistic effect.

[0041] Table 1 Results of indoor joint toxicity determination of the mixture of compound I-72 and organophosphorus insecticides such as chlorpyrifos against Frankliniella occidentalis

[0042]

[0043]

[0044] Example 2: Indoor combined toxicity test of compound I-72 and organophosphorus insecticides such as chlorpyrifos against Chilo suppressalis

[0045] Test target: 3rd instar larvae of the Chilo suppressalis [Myzus persicae (Sulzer)], a susceptible strain reared indoors.

[0046] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0047] Experimental method: First, select fresh and tender rice seedlings. The experimental design is from low dose to high dose. Put them into the prepared solution and immerse them for 10 seconds. After natural drying in the shade, cut them into small sections of 5 cm and place them in a culture dish with a diameter of 9 cm and filter paper. Inoculate neatly packed healthy test insects. 10 heads are placed in each treatment, and each treatment is repeated 3 times. A blank control is also set up.

[0048] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 2.

[0049] Table 2 Results of indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against Chilo suppressalis

[0050]

[0051]

[0052] Example 3: Synergistic effect test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos on Plutella xylostella. Test target: Plutella xylostella, 3rd instar larvae.

[0053] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0054] Experimental method: First, fresh cabbage leaves grown in the greenhouse were selected, and circular leaflets with a diameter of 3 cm were punched out of the cabbage leaves. According to the experimental design, the leaves were placed in the prepared solution and immersed for 10 seconds in the order from low dose to high dose. After natural drying, they were placed in a culture dish with a diameter of 9 cm and filter paper, and neatly arranged healthy test insects were inoculated. Ten insects were treated for each treatment, and each treatment was repeated 3 times. A blank control was also set up.

[0055] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 3.

[0056] Table 3 Results of indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against Plutella xylostella

[0057]

[0058] Example 4: Indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against cotton aphids

[0059] Test target: cotton aphid (Aphis gossypii Glover) nymphs, a susceptible strain reared indoors.

[0060] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0061] Test method: First, select cotton leaves with neat and consistent cotton aphids, with each leaf carrying about 20 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0062] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 4.

[0063] Table 4 Results of indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against cotton aphids

[0064]

[0065]

[0066] Example 5: Synergistic effect test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos on Bemisia tabaci. Test target: nymphs of Bemisia tabaci Gennadius.

[0067] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0068] Experimental method: First, select cotton leaves with neat and consistent whitefly nymphs and make leaf discs. Each leaf disc carries about 20 insects. Then, spray them evenly in the order from low dose to high dose according to the experimental design, 1.5 mL per leaf, and repeat each treatment 3 times. Set up a blank control.

[0069] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 96 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei's method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 5.

[0070] Table 5 Results of indoor combined toxicity test of compound I-72 and organophosphorus insecticides such as chlorpyrifos against Bemisia tabaci

[0071]

[0072] Example 6: Indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against yellow-striped flea beetles

[0073] Test target: Adult yellow-striped flea beetles (Phyllotreta spp), a susceptible strain reared indoors.

[0074] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0075] Experimental method: First, select fresh cabbage seedlings grown in the greenhouse, and then spray them evenly in the order from low dose to high dose according to the experimental design. After natural drying, cover them with glass covers and inoculate neatly arranged healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0076] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 6.

[0077] Table 6 Results of indoor combined toxicity test of compound I-72 mixed with chlorpyrifos and three other organophosphorus insecticides against yellow striped flea beetle

[0078]

[0079] Example 7: Synergistic effect test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos on Hyphantria cunea Test target: 3rd instar larvae of Hyphantria cunea, sensitive strain reared indoors.

[0080] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0081] Experimental method: First, select fresh and tender poplar leaves and cut them into small sections of 5 cm. The experimental design is from low dose to high dose. Put them into the prepared solution and immerse them for 10 seconds. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate them with neatly arranged healthy test insects. Each treatment has 10 heads and each treatment is repeated 3 times. A blank control is also set up.

[0082] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei's method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 7.

[0083] Table 7 Results of indoor combined toxicity test of compound I-72 and three organophosphorus insecticides including chlorpyrifos against the nymphalidae

[0084]

[0085]

[0086] Example 8: Indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against corn borer

[0087] Test target: 3rd instar larvae of corn borer (Ostrinia furnacnlis Guenee), a susceptible strain reared indoors.

[0088] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0089] Experimental method: First, select fresh corn stalks grown in the greenhouse, cut them into small sections of 3 cm, and design the experiment in the order from low dose to high dose. Put them into the prepared solution and immerse them for 10 seconds. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate them with neatly arranged healthy test insects. Each treatment has 10 heads and 3 replicates. A blank control is also set up.

[0090] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 8.

[0091] Table 8 Results of indoor combined toxicity test of compound I-72 mixed with organophosphorus insecticides such as chlorpyrifos against corn borer

[0092]

[0093]

[0094] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified in several ways, and these improvements and modifications also fall within the protection scope of the claims of the present invention. It can be seen from the above technical scheme that the insecticide and acaricide composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing the amount of pesticides used, reducing the amount of pesticide residues on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and pests are not easy to develop drug resistance. The composition of the present invention can be applied to the prevention and control of various pests and mites of various crops, and can be widely used in the field of agriculture.

Claims

1. A binary synergistic compound insecticide composition, characterized in that: The composition is composed of two active ingredients A and B, wherein the active component A is selected from a compound having the following structure, and the active component B is selected from an organophosphorus insecticide or acaricide; the weight ratio of the two active components is 100:1 to 1:100; The compound has the following structure: The organophosphorus insecticide and acaricide are selected from acephate, chlorpyrifos, diazinon, phoxim, triazophos and malathion.

2. The insecticide composition according to claim 1, characterized in that: The weight ratio of the two active components is 50:1 to 1:

50.

3. The insecticide composition according to claim 2, characterized in that: The weight ratio of the two active components is 20:1 to 1:

20.

4. The insecticide composition according to claim 2, characterized in that: The weight ratio of the two active components is 10:1 to 1:

10.

5. The binary insecticide composition according to claim 1 can be used for controlling pests, mites and nematodes in agriculture, forestry, fruit, vegetable, tea and the like, wherein the pests, mites and nematodes are one or more of western flower thrips, striped stem borer, diamondback moth, aphid species, whitefly species, yellow flea beetle, gypsy moth and corn borer.

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