The invention discloses a freeze-
drying method of long
culture growth hormone, which comprises the following steps: adding a middle plug into a double-cavity clamping
bottle, filling the double-cavity clamping
bottle with the long
culture growth hormone to be freeze-dried, and placing and fixing the double-cavity clamping
bottle on a customized freeze-
drying bracket; the top plug is placed in the plug pressing
assembly so as to be fixed to the bottle opening of the double-cavity clamping type bottle; performing freezing, primary
drying and secondary drying on the to-be-freeze-dried long
culture growth hormone respectively; the top plug is fixed by the top cover and the bottle opening is sealed; the plug pressing
assembly comprises a pressing-in jig and a maintaining jig, the maintaining jig is attached to the outer wall of the bottle opening, and the top plug is placed in the maintaining jig so that the top plug can be fixed to the bottle opening; the press-in jig presses the top plug to be embedded into the bottle opening, and the plug pressing
assembly is removed to complete plug pressing. According to the method disclosed by the invention, high-quality freeze-drying parameters and freeze-drying operation can be provided, and the top plug and the top cover are utilized to complete plug pressing and cover sealing of the double-cavity clamping type bottle, so that the obtained
growth hormone freeze-drying preparation for the growth culture keeps better appearance and lower
moisture.