75 results about "Omethoate" patented technology

The invention discloses an antibody preparation method through which various organic phosphorus pesticide residues can be detected and the application, which belongs to the technical filed of agriculture. The antibody preparation method adopts the steps that firstly, O,O-diethylthiophosphoryl chloride is used to react with 4-aminobutyric acid to synthesize into general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP which comprises various organic phosphorus pesticide common structures-O, O-diethyl thiosulfate phosphoryl; secondly, the general hapten O, O-diethyl-N-(3-carboxy-propyl)-by-sulfur Phosphoramidate DENP is respectively coupled with bovine serum albumin BSA and chicken ovalbumin OVA by adopting carbodiimide method, to prepare general immunogen DENP-BSA and general coatingen DENP-OVA; and thirdly, multi-clonal antibody is prepared through immunized New Zealand white rabbits, the obtained antibody has obvious specificity to various organic phosphorus pesticides, such as thimet, demeton, disulfoion, omethoate, phoxim and quinalphos and can be used for the immune detection of the organophosphorus pesticides.

The invention relates to a method for detecting ten types of organophosphorus pesticides by using magnetic separation-gas chromatography. The method is used for quickly detecting ten types of organophosphorus pesticides (dipterex, methamidophos, acephate, omethoate, moncrotophos, dimethoate, phosphamidon, parathion-methyl, malathion and chlorpyrifos) based on combination of solid-phase extraction, magnetic separation and the gas chromatography. The method comprises the following steps: preparing a magnetic solid-phase extraction agent, establishing a combination system of the magnetic separation and the gas chromatography, and detecting the organophosphorus pesticides in objects to be detected. A method capable of quickly separating and detecting ten types of organophosphorus pesticides at high sensitivity simultaneously is established by taking prepared magnetic chitosan microspheres with high absorption performance on the ten types of organophosphorus pesticides such as dipterex and the like as adsorption functional materials and using combination of the solid-phase extraction, magnetic separation and the gas chromatography. The method is simple in experimental operation, high in detection sensitivity, low in cost and applicable to detection of residues of ten types of organophosphorus pesticides such as trace dipterex and the like in all products.

The invention provides a detection method for omethoate on the basis of a CsPbBr3 perovskite quantum dot-molecular imprinting fluorescence sensor. The detection method comprises the following steps: S1: preparing the CsPbBr3 perovskite quantum dot-molecular imprinting fluorescence sensor; S2, drawing a standard work curve of fluorescence quenching intensity-omethoate concentration, and working outa working equation; S3, measuring the content of omethoate in a sample. The detection method provided by the invention achieves high selectivity and high sensitivity detection to the omethoate by using an APTES (aminopropyltriethoxysilane) blocked CsPbBr3 quantum dot as a signal element, and then wrapping with the CsPbBr3 perovskite quantum dot-molecular imprinting fluorescence sensor with a sensitive omethoate recognition function on a molecularly imprinted polymer layer. The detection method provided by the invention has a linear detection range of 50-400 ng / mL, a linearly dependent coefficient of 0.997, a limit of detection of 18.8ng / mL and relative standard deviation of 1.7 percent (RSD).

The invention discloses a soil remediation device capable of removing omethoate components in farmland soil. The device comprises an original soil hopper, a blast sorting machine, a vibrating screening machine, a magnetic screening machine, a grinding machine, a three-stage microbe spraying and degrading reactor, an adjusting type spray tower, an electric heating blast dryer and a remedied soil storage silo. The device has the advantages that by creatively utilizing microbial degradation reaction of bacillus cereus, with participation of transphosphorylase, C-P bonds in the main components, O,O-dimethyl-S-[2-(methylamino)-2-oxo-ethyl] thiophosphate molecules, in omethoate, are broken and the main components are gradually degraded into harmless simple compounds, such as CO2, N2 and H2O, in a temperature range of 35-38 DEG C; the soil purified through microbial degradation can be repeatedly applied to agricultural production; meanwhile, no toxic chemical substances are used or generated in a method, thus basically putting an end to secondary harm to the environment in the soil remediation process.

The invention discloses a pesticide. The pesticide comprises, by weight, 20 to 30 parts of omethoate, 10 to 15 parts of esbiothrin, 30 to 40 parts of imidacloprid and 1000 parts of water. The invention has the following beneficial effects: since materials used in the invention are cheap, the pesticide has low production cost; and the pesticide has a good insecticidal effect and is suitable for large-scale popularization and application.

The invention provides a disease and pest prevention method for sasanqua, and relates to the technical field of sasanqua planting management. The disease and pest prevention method for the sasanqua is characterized in that the method includes the following steps that sasanqua anthracnose is prevented, from July to September, when the saplings planted for one to two years are in a peak period of diseases, the saplings are sprayed with 1% Bordeaux mixture plus 2% tea bran water three times to four times; leaf eating pests are prevented, phototaxis of adult pests is utilized to trap and kill the pests through lamp light, and 1000-1500-time liquid of dipterex and dichlorvos can be sprayed in the larval phase; branch damaging pests and fruit damaging pests are prevented, feign death of the pests is utilized, manual culling is conducted or 1000-time liquid of 90%-dipterex is sprayed to kill the pests, and 1000-time liquid of 40%-omethoate is sprayed to kill scale insects from March to April. The method is good in prevention effect and not prone to recurring, biological prevention is combined with chemical prevention and thus pollution is smaller.

The invention relates to the technical field of microbe application, particularly to an aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application of the aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity to dissolution of insoluble potassium, organophosphorus and inorganic phosphorous, decomposition of organophosphorus pesticides and preparation of microbial fertilizers. The aspergillus niger JXJ A01 is stored in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 13775. The aspergillus niger JXJ A01 is high in dissolving effects on insoluble phosphorous and potassium ore powder and can convert the insoluble phosphorous and the potassium ore fertilizer into soluble phosphorous and potassium absorbable for plants and degrade the organophosphorus pesticide of omethoate into non-toxic soluble phosphate radicals to reduce pesticide concentration and increase the content of soluble phosphorous in soil. The aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity can be applied to increasing the content of soluble effective potassium and phosphorus in soil and repair pollution caused by the organophosphorus pesticides to soil.

The invention discloses bacillus subtilis BLCC1-0388, which is collected in China Center for Type Culture Collection, and the collection number of the bacillus subtilis is CCTCC NO:M 2015503. The invention also discloses application of the bacillus subtilis in degrading organophosphorus pesticide and a product taking the bacillus subtilis as an effective component. The bacillus subtilis disclosed by the invention has a better degradation effect on the organophosphorus pesticide, especially has a remarkable degradation effect on omethoate, and has a broad application prospect in sustainable agricultural development; moreover, the strain of the bacillus subtilis disclosed by the invention can be planted and bred in soil under the condition that only the soil exists, and is capable of degrade the omethoate.

The present invention relates to pesticide technology, and is recombinant acetylcholinesterase and its preparation process and usage in detecting pesticide residue. Recombinant acetylcholinesterase may be dissolved in buffering sodium phosphate solution and after being maintained at 4 deg.c for 22 days, it has 75 % of its initial enzyme activity maintained. It has inhibiting constant on DDVP, omethoate, paraoxon, methyl paraoxon, aldicarb, carbaryl, carbofuran and aphox of 4.4E6, 2.2E4, 1.1E6, 4.2E5, 4.8E4, 5.9E5, 5.1E6 and 2.3E5 separately. It is prepared through selecting seed, shaking cultivation, centrifuging, filtering, depositing, leaching, stirring, dialyzing and freeze drying, and is maintained at -20 deg.c. The detection process includes preparing reagent, treating sample, detecting sample, blank testing, testing sample, and final calculating. The recombinant acetylcholinesterase has obvious sensitivity on organophosphorus pesticide and carbamate chemicals and may be used in detecting pesticide residue.

The invention discloses a comprehensive control method for walnut saturnidae and inchworm. The method comprises the following steps: (1) spraying a pesticide onto leaves in a larval phase, namely spraying a solution in an amount which is 3,000 times volume of 2.5 percent lambda-cyhalothrin missible oil, a solution in an amount which is 500 times volume of 25 percent chlorbenzuron, a solution in an amount which is 1,500 times volume of 80 percent dichlorvos missible oil or a solution in an amount which is 4,000 times volume of 2.5 percent Emamectin benzoate missible oil in the larval phase; and (2) applying a pesticide through trunk injection in a growth phase, namely injecting a systemic pesticide such as omethoate or a dimehypo liquid medicine into trunks, so that the liquid medicine is slowly injected into trees, active ingredients are absorbed into tissues through a systemic conduction function, and pests are poisoned after eating the liquid medicine. By a method of combining spraying and trunk injection, the walnut saturnidae and the inchworm are well controlled.

The invention discloses an aptamer wrapped gold nanoparticle probe-based reagent for the colorimetric detection of omethoate and application of the reagent. The reagent is prepared by the following steps of adding an aptamer solution at concentration of 2 to 5 mu M into a gold nanoparticle solution at concentration of 1 to 3nM, performing reaction for more than 1h, performing centrifugation at 8,000 to 12,000rpm, and performing resuspension to obtain the reagent by using a PBS solution at concentration of no higher than 0.1M, wherein a sequence of an aptamer is 5minute-AGCTTGCTGCAGCGATTCTTGATCGCCACAGAGCT-3minute. When the reagent is adopted for detecting omethoate, the lower limit of detection is lower than 0.2 mu M. Compared with a conventional omethoate detection reagent, the reagent is higher in sensitivity.

The invention discloses a whiteflower hogfennel root Chinese herbal medicinal yield-increasing fertilizer, which consists of dried duck droppings, dried cow droppings, cornmeal, sorghum flour, bran, soybean stem meal, cottonseed hulls, potash fertilizer, urea, a nitrogen fertilizer, glucose powder, omethoate and polyurethane. The yield-increasing fertilizer is prepared from the following steps of: totally combining all the powder and stirring in a stirrer for 90 minutes; taking out and grinding into 80-mesh granules in a grinder; stirring the granules in the stirrer for 180 minutes, taking all the granules out and pouring into a fermenting tank; and adding fecal water according to a ratio of 1:1.5, uniformly stirring up and down, and piling up; tamping, covering with plastic cloth tightly, fermenting for 30 hours, and turning piles for the first time; adding the fecal water again according to a ratio of 1:1.5, uniformly stirring up and down, piling up, tamping, covering with plastic cloth tightly, fermenting for 50 hours, and turning the piles for the second time; and piling up and fermenting for 72 hours so as to obtain the fertilizer. The fermented yield-increasing fertilizer is put into a closed die to form yield-increasing fertilizer blocks, namely the qualified yield-increasing fertilizer.

The invention provides a Lactobacillus plantarum P9 with pesticide degrading activity, and the deposited number is CGMCC No.16401. The Lactobacillus plantarum P9 has a pesticide degrading activity. Plant milk stem P9 can degrade organophosphorus pesticides such as omethoate, methamidophos and dimethoate, and has good fungal inhibition, especially against Phytophthora melonis. Therefore, it can beused as a vegetable and fruit detergent or agricultural biological agent and has the effect of degrading organophosphorus pesticides, removing organophosphorus pesticide residues and inhibiting mouldactivity. Because of its acid resistance, bile acid resistance, catalase and protease stability, it can survive in the gastrointestinal organs of animals, including humans, so it can be used as a foodor food additive, probiotic bacteria with probiotic activity.

The invention relates to a pesticide, and particularly relates to a pesticide for prevention of plant hopper and boring insect and other injurious insects. The pesticide is characterized by comprising the following components in parts by weight: 10 parts of salicylic acid, 10 parts of stearic acid, 15 parts of omethoate, 15 parts of toluene, 10 parts of triazophos, 10 parts of sodium alkyl benzene sulfonate, 10 parts of bentonite and 10 parts of castor oil polyester vinyl ether, and the pesticide is safe and efficient, strong in permeability, little in pollution and strong in wide spectrum property.

The invention relates to an insecticide. The insecticide comprises the following components in parts by weight: 20-30 parts of omethoate, 5-10 parts of methamidophos, 10-15 parts of esbiothrin, 30-40 parts of imidacloprid, 10-20 parts of sodium hydroxide and 3-8 parts of an inductive agent. The insecticide provided by the invention is effective to various kinds of insects and has the advantages of wide range, good drug properties and difficulty in generation of drug resistance.

The invention relates to a pesticide omethoate degrading microorganism compound bacterial agent and a preparation method thereof. The compound bacterial agent is prepared from Pseudomonas graminis ZZY-C13-1-8 or a mixture of the Pseudomonas graminis ZZY-C13-1-8 and Pseudomonas plecoglossicida, glycerin, activated carbon and kaoline with a mixing ratio being 1 to 1 to (1.5-2) to 3 to (2.5-3). A thallus involved by the invention is cultured by a special fluid nutrient medium, the obtained compound bacterial agent can degrade omethoate residual in agricultural soils in situ with the degrading ratio of the omethoate residual in the soils reaching 75 percent or above, and the pesticide omethoate degrading microorganism compound bacterial agent and the preparation method have the advantages of low treatment cost, simpleness in construction, no influence on production and operation, no secondary pollution, small influence on environment and the like.

The invention discloses a sunflower pesticide, which comprises the following components in parts by weight: 30 to 50 parts of bark of Chinese redbud, 10 to 14 parts of stellera chamaejasme, 30 to 50 parts of octadecanol polyoxyethylene ether, 30 to 40 parts of imidacloprid, 70 to 90 parts of gentiana scabra, 30 to 50 parts of radix rehmanniae, 70 to 80 parts of chlorhexidine, 80 to 90 parts of geranium wilfordii maxim, 50 to 70 parts of lithospermum, 20 to 40 parts of glycerol, 40 to 60 parts of omethoate, 20 to 40 parts of dahurian angelica root, and 1600 to 2000 parts of water. The provided pesticide is a plant-origin pesticide, is environment-friendly, and is harmless to people's health, and no drug resistance is generated.

The invention discloses a quantum dot-MXene fluorescence sensor as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: (1) preparing a Glu-His-GQDs product; (2) preparing an aptamer connector; (3) preparing an MXene solution; and (4) preparing the graphene quantum dot-MXene fluorescence sensor. The fluorescence sensor prepared by the invention has the characteristics of easiness in synthesis, low cost, high light stability and low toxicity, has high sensitivity and high specificity on omethoate, and has wide application prospects in environmental monitoring and food safety monitoring in the market.

The invention discloses a prevention method of plum leaf curl. The method comprises the concrete steps of (1) heating omethoate with the concentration being 40 percent to 80 to 85 DEG C, and keeping for 3 to 5min; (2) mixing carbendazim with the concentration being 50 percent and ethyl alcohol with the concentration being 60 percent according to the mass ratio of 3 to 1, and obtaining carbendazim-ethyl alcohol mixed liquor; (3) mixing the omethoate liquor and the carbendazim-ethyl alcohol mixed liquor according to the mass ratio of 1 to 2; (4) arranging an atomizing nozzle inside a crown of a plum tree, then spraying the mixed pesticide liquor through the atomizing nozzle, and applying the pesticide liquor to the back face of a plum leaf; (5) after 5 to 7d of spraying the pesticide liquor, spraying clean water once again. According to the prevention method of the plum leaf curl provided by the invention, innovative raw materials are adopted, and the pesticide liquor can accurately arrive to a concentrated area of pathogenic bacteria through a unique processing method and spraying method, so that an optimal sterilizing effect is achieved.

The invention discloses a special insecticide special for piercing-sucking mouthpart insects and a preparation method for the insecticide. The insecticide consists of the following components in parts by mass: 20 to 30 parts of dichlorvos, 18 to 21 parts of omethoate, 15 to 17 parts of fenitrothion, 10 to 13 parts of decis, 11 to 12 parts of supracide, 7 to 9 parts of dicofol, 8 to 10 parts of anaphicide, 5 to 7 parts of dithane, 3 to 5 parts of octyl mercaptan, 5 to 8 parts of imidacloprid, 3 to 5 parts of Lvling, 5 to 7 parts of pymetrozine, 1 to 3 parts of java paecilomyces, 2 to 4 parts of beta-cypermethrin and 20 to 30 parts of water. The special insecticide for the piercing-sucking mouthpart insects has good stomach poisoning and contact effects, superstrong uptake and translocation performance and stable, persistent and high efficacy.

The invention discloses a high-efficiency pesticide. The high-efficiency pesticide is prepared from the following raw materials, by weight, 15-18 parts of omethoate, 15-20 parts of imidacloprid, 5-7 parts of glycerol, 10-12 parts of methamidophos, 3-5 parts of ethylene diamine tetraacetic acid, 9-12 parts of alkyl sodium sulfonate and 8-9 parts of triphenyl phosphate. The insecticidal effects are good, the penetration capability is strong, tenacious pests in crops can be eliminated, residues on surfaces of crops are small after the pesticide is used, the environmental protection capability is strong, the pesticide has little harm to human body, and the cost is low.

The invention relates to an osier foliar fertilizer. The osier foliar fertilizer consists of the following components: urea, sodium molybdate, copper sulfate, manganese chloride, zinc sulfate, sodium borate, triadimefon, omethoate, magnesium chloride, humic acid, lauryl sodium sulfate and a chelating agent. The osier foliar fertilizer disclosed by the invention can be used for providing microelements for the growth of osiers, also preventing plant diseases and insect pests of the osiers and increasing the fertilizer absorptivity of leaf surfaces.

The invention discloses a method for rapidly identifying recessively added forbidden and limited pesticides in a fertilizer. The method comprises forbidden pesticide methamidophos and limited pesticide omethoate. The method comprises the following steps: weighing a solid fertilizer or a liquid fertilizer, dissolving with ultrapure water, adding acetonitrile and sodium chloride, violently mixing, standing for layering, and taking supernate; carrying out sample application on the supernate on a thin-layer plate by using a capillary tube, developing by using petroleum ether, dichloromethane and methanol, and developing by using palladium chloride; rapid identification can be carried out according to Rf values and color development of methamidophos and omethoate, the color development time of methamidophos is 2 min, the color shows deep yellow, and Rf is 0.36; the color development time of omethoate is 10 min, the color is light yellow green, and Rf is 0.41. The identification method disclosed by the invention is simple and convenient to operate, does not need large-scale pretreatment equipment, is used for on-site rapid detection of solid fertilizers or liquid fertilizers, and can rapidly identify two forbidden and limited pesticides, namely methamidophos and omethoate, which are recessively added into the fertilizers only by judging color development and Rf values with naked eyes.

The invention discloses a long-acting insecticide. The long-acting insecticide comprises the following constituents in parts by weight: 5 to 10 parts of aromatic hydrocarbon fluid, 3 to 7 parts of tetramethrin, 2 to 6 parts of stearic acid, 5 to 12 parts of toluene, 3 to 10 parts of omethoate, 9 to 20 parts of potassium chloride, 5 to 10 parts of menthol crystal, 3 to 10 parts of potassium sulfate, 5 to 12 parts of tincal, and 4 to 10 parts of triazophos. The long-acting insecticide is reasonable in formula, good in use effect, low in production cost and good in insecticidal efficacy, does not pollute the environment, is safe, efficient, good in permeability, rapid to exert effect, high in insecticidal efficacy, and harmless to a human body.

The present invention relates to an efficient and non-toxic pesticide for controlling red spiders on fruit trees. The pesticide comprises asarum, capsicum and zanthoxylum bungeanum. By using botanical drugs, the pesticide is environmentally-friendly, non-toxic, free of pesticide residues and quick in effect-taking; the synergism of three botanical drugs has good effect of controlling red spiders, the pesticide even has better effect of controlling red spiders than that of omethoate and does not generate drug resistance.

The invention discloses an environment-friendly insecticide composition, which is prepared from the following raw materials in parts by weight: 3 to 8 parts of Chinese herbal medicine extraction liquid, 0.3 to 0.7 part of trichloroisocyanuric acid, 2 to 6 parts of pyrethrum, 0.1 to 0.5 part of lysine, 0.1 to 0.4 part of fructus cnidii, 2 to 7 parts of pepper powder, 0.3 to 0.6 part of edetic acid,2 to 6 parts of abamectin, 3 to 7 parts of ligusticum oil, 0.3 to 0.8 part of edetic acid, 3 to 7 parts of edible plant oil, 4 to 8 parts of herba patriniae, 3 to 8 parts of herba celastri angulati,2 to 7 parts of garlic powder, 3 to 9 parts of aspirin, 2 to 7 parts of emulsifiers, 0.3 to 0.7 part of triphenyl phosphate, 0.2 to 0.6 part of cypermethrin, 0.3 to 0.8 part of crotin, 2 to 6 parts offolium eucalypti robustae, 0.2 to 0.5 part of acephate, 0.3 to 0.6 part of glycerol, 0.3 to 0.7 part of tobacco leftovers, 2 to 6 parts of omethoate, 0.3 to 0.7 part of agritol, 0.3 to 0.7 part of potassium chloride and 0.3 to 0.7 part of ethanol ethyl ester. The environment-friendly insecticide composition uses traditional Chinese medicine as major raw materials; the characteristics of no medicine damage, no residue and no environment pollution are realized; the advantages of high killing effect on pest, high safety and the like are realized.

The invention relates to the technical field of application of microorganisms, and in particular to bacillus capable of degrading slightly soluble phosphorus and an organophosphorous pesticide. The bacillus is characterized in that the bacillus is bacillus JXJ b05, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.14050. The bacterium (the bacillus) can take a relatively good dissolving activity on such slightly soluble phosphorus in the form of calcium phosphate, calcium phytate and the like, and the bacterium can degrade the organophosphorous pesticide, namely omethoate, so that the omethoate is converted into a soluble phosphate radical; therefore, the concentration of the pesticide is reduced; and the bacterium can be used for increasing soil soluble effective phosphorus fertilizer and repairing pollution of the organophosphorous pesticide on soil.

The invention belongs to the technical field of pesticides and relates to a composite pesticide for succulent plants, comprising effective constituents having a weight ratio: 1:10-10:1, namely omethoate and Epimedium extract. The Epimedium extract and the omethoate are composited, sensory nerves of pests are stimulated by taking advantage of the Epimedium extract, the pests are kept excited during spraying of the composite pesticide and died of inhaling enough omethoate, omethoate utilization is increased without increasing the omethoate, cost is lowered, environmental pollution is decreased; pest damage can be effectively prevented, and the pest preventive effect of the composite pesticide is up to above 95%; as the consumption of and spray times of the omethoate are decreased, a safety environment is provided for the growth of succulent plants.

The invention provides a multifunctional insecticide. The insecticide is prepared from, by weight, 1-3 parts of alum, 3-7 parts of bancoul nuts, 7-12 parts of russula emetica, 10-14 parts of methanol,5-9 parts of imidacloprid, 1-3 parts of omethoate, 2-6 parts of Chinese lobelia herb, 4-8 parts of roots of stellera chamaejasme, 3-6 parts of garlic, 4-9 parts of dispersant, 1-3 parts of acetamiprid and 7-11 parts of roots of tripterygium wilfordii. The insecticide has wide range of application, simple production process and high product stability, the application range is wide, and the insecticide has strong insecticidal effect and less environmental pollution.

The invention provides a method for measuring the pesticide resistance of macrosiphum avenae. The method comprises the following steps of manufacturing a diagnosis glass pipe under the diagnosis dosage of a pesticide, rapidly detecting the resistance level to the pesticide from macrosiphum avenae field population, wherein the pesticide is imidacloprid, pirimor, omethoate or beta-cypermethrin, and the diagnosis dosage of the pesticide comprises 4289ng / cm<2> of the imidacloprid, 103ng / cm<2> of the pirimor, 224ng / cm<2> of the omethoate, and the 4695ng / cm<2> of the beta-cypermethrin. According to the method provided by the invention, the use is simple and convenient, the result is intuitive, the method is suitable for rapidly detecting macrosiphum avenae resistance level in the field, the disadvantages that the number of samples in the traditional detecting method is large, the operation is tedious, the time is wasted and the like are overcome, and the method is used for rapidly detecting the macrosiphum avenae field resistance level.