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Bacillus capable of degrading slightly soluble phosphorus and organophosphorous pesticide

A technology of organophosphorus pesticides and bacillus, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of easy death of microorganisms, unstable quality, short survival period, etc., and achieve improvement and restoration of farmland soil , reduce the effect of pesticide residues

Active Publication Date: 2018-01-12
JIUJIANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At the same time, at present, microbial fertilizers generally have shortcomings such as short survival period, unstable quality, and easy death of microorganisms during fertilizer production and processing.

Method used

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  • Bacillus capable of degrading slightly soluble phosphorus and organophosphorous pesticide
  • Bacillus capable of degrading slightly soluble phosphorus and organophosphorous pesticide
  • Bacillus capable of degrading slightly soluble phosphorus and organophosphorous pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] 1 Screening of Bacillus phosphate

[0023] Solid plate screening: the soil collected from the peanut field in Wuning County, Jiangxi Province was placed in an electric drying oven at 100°C for heat treatment for 1 hour, and then gradiently diluted with sterile water, and an appropriate gradient dilution was taken in the bacterial culture medium (glucose 0.4% , malt extract powder 0.5%, yeast extract powder 0.4%, agar 1.8%, pH 7.4, water 1000mL), and placed in a 28 ° C incubator for 1-2 days, take a single colony for streaking Purify further, adopt the "cross" method to inoculate the purified bacterial strain on the phosphate-dissolving solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl0.3g, MgSO 4 ·7H 2 O 0.3g, KCl 0.3g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, calcium phosphate or calcium phytate 10g, agar 18g, distilled water 1000mL, pH 7.0), and placed in a 28°C incubator for 2-7d, observe the presence and size of the phosphorus-dissolving circle, and sel...

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Abstract

The invention relates to the technical field of application of microorganisms, and in particular to bacillus capable of degrading slightly soluble phosphorus and an organophosphorous pesticide. The bacillus is characterized in that the bacillus is bacillus JXJ b05, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.14050. The bacterium (the bacillus) can take a relatively good dissolving activity on such slightly soluble phosphorus in the form of calcium phosphate, calcium phytate and the like, and the bacterium can degrade the organophosphorous pesticide, namely omethoate, so that the omethoate is converted into a soluble phosphate radical; therefore, the concentration of the pesticide is reduced; and the bacterium can be used for increasing soil soluble effective phosphorus fertilizer and repairing pollution of the organophosphorous pesticide on soil.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a bacillus capable of degrading insoluble phosphorus and organophosphorus pesticides. Background technique [0002] Phosphorus is an indispensable element for the normal growth and development of plants. More than 95% of the phosphorus in the soil is insoluble phosphorus, which is difficult for plants to directly absorb and utilize. About 74% of the cultivated land in my country is deficient in phosphorus. The application of organic and chemical phosphorus fertilizers is the main source of soil phosphorus. However, soil minerals have a strong reactivity to phosphorus, and are often prone to form insoluble and ineffective phosphates, resulting in a seasonal utilization rate of only 5% to 25% of the applied phosphate fertilizer. Phosphate fertilizer mainly comes from phosphate rock powder, but phosphate rock powder is a non-renewable resource and will be consumed...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/26A62D101/45C12R1/07
Inventor 张炳火李汉全
Owner JIUJIANG UNIVERSITY
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