158 results about "Soil phosphorus" patented technology

The invention provides a penicilliums p. strain and application thereof. Eucalyptus endophyte is a penicilliums p. strain 1, and the collection number is CGMCC No.4297. The eucalyptus endophyte inoculated to eucalyptuses can improve the low phosphorus stress resistance of the eucalyptuses and promote phosphorus absorption of the eucalyptuses. Because the eucalyptus endophyte is used for promoting the phosphorus absorption of the eucalyptuses, and when the strains are inoculated to the eucalyptus plants, the activities of the plant phosphatase and the soil phosphatase can be improved, the pH value of the soil rhizosphere can be reduced, and the plant P utilization rate is greatly improved; therefore, the purpose of promoting normal growth of the plants in the low phosphorus soil is fulfilled, and the utilization rate of the soil phosphate fertilizer is greatly reduced.

The invention provides efficient phosphate solubilizing growth-promoting bacteria and a microbial agent prepared from the same. The active ingredients of the microbial agent are pantoea ananatis P21 and hirsutella minnesotensis. According to the invention, the widely-applicable efficient phosphate solubilizing bacteria pantoea ananatis P21 and fungi for efficiently preventing nematodiasis are organically combined; and by utilizing the composite agent provided by the invention, the soil phosphorus is efficiently used, the utilization rate of phosphate fertilizer is improved, the function of microorganism in preventing nematodiasis is sufficiently realized, and an effect of increasing yield by solubilizing phosphate and preventing diseases is realized, thereby having great significance to sustainably increasing grain yield of the country, saving phosphate fertilizer resource, reducing nematodiasis and protecting the ecological environment of farmland.

The invention discloses a soil phosphor activator and using method, which comprises the following parts: 30-50% complex builder H2, 30-40% humic acid HA and 10-40% filler, wherein the complex builder H2 is alkaline paper making waste-liquid drier.

The invention discloses a bacillus subtilis HL-1 and an application thereof in the respect of soil phosphate dissolving. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on Aug 24th, 2011, with a collection number of CGMCC No.5175. The 16SrDNA nucleotide sequence of the strain is represented by SEQ ID NO:1. With added diatomite and guava slag adsorbing agent materials, a fermentation broth of the bacillus subtilis HL-1 can be prepared into microbial manure. With the strain provided by the invention, phosphate availability and phosphate fertilizer utilization rate of the soil can be improved, plant growth can be promoted, and soil microbe diversity can be improved. The strain has important significance in reducing chemical fertilizer application, modifying soil micro-ecology, and promoting soil sustainable utilization. The strain has wide application prospect in production practice.

The invention provides efficient phosphorus-dissolution promotion bacteria and a microbial agent prepared from the same. The effective components of the microbial agent include Pantoea agglomerans P36 and Trichoderma harzianum. In the invention, the widely-adaptable efficient phosphorus-dissolution yield-increasing bacteria Pantoea agglomerans P36 and the efficient anti-crop disease Trichoderma harzianum are organically combined; by applying the composite bacterial agent provided by the invention, the effect of the microorganism in preventing soil-borne diseases is sufficiently exerted while efficiently utilizing the soil phosphorus and improving the utilization rate of phosphate fertilizer; an effect of increasing yield by dissolving phosphorus and resisting diseases is realized; and theinvention has tremendous application values in agriculture.

This invention discloses a soil phosphorus activation agent, which is composed of: aspergillus niger phosphorus-dissolving bacteria 35-45 wt.%, peat coal 30-35 wt.%, corn flour 8-12 wt.%, lees 6-8 wt.%, allantion 1-3 wt.%, and ammonium nitrohumate 7-9 wt.%. The soil phosphorus activation agent is produced by: preparing aspergillus Niger phosphorus-dissolving bacteria, preparing the adsorption agent, mixing, and piling. This invention provides a high-activity, high-efficiency and no pollution green agricultural product. The soil phosphorus activation agent can improve the phosphorus-supplying capacity of soil to crops, lower production cost, and increase crop productivity.

The invention provides an autonomously-screened efficient phosphate-dissolving bacterium and a produced bacterial agent thereof, and belongs to the technical field of biology. The bacterial strain of the phosphate-dissolving bacterium is named as 21-III, is identified as burkholderia sp., and is preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 7873. 16SrDNA sequence of the bacterial strain is submitted to GenBank, and the registration number is JX857813. The main biological characteristics of the bacterial strain comprise that Gram staining is negative, thallus is in the shape of a rod, catalase reaction is positive, oxidase reaction is positive and methyl red reaction is negative. The bacterial agent has obvious promotion effect on dissolution rate of phosphorus in soil, and is applicable to improve soil fertility of crops and beneficial for environment protection.

A production method of an environment-friendly slow-release fertilizer, comprising mixing slurry, granulating, drying, cooling, and screening, adding activated humic acid and polyaspartic acid into molten urine, and fully mixing , the obtained mixed slurry is used as the liquid phase of compound fertilizer granulation, and sent to the granulator for granulation. The slow-release fertilizer product produced by the present invention has a good effect. Active humic acid and polyaspartic acid are added to the molten urine, which improves the low nitrogen utilization rate and can play a nitrogen synergistic effect. The availability of phosphorus and potassium in the soil can stimulate crop growth, provide more comprehensive nutrition, and significantly increase production.

The invention discloses efficient disease-resistant pralidoxime bacterial manure for tobacco and a production method thereof, relates to bacterial manure, and simultaneously relates to a method for producing the bacterial manure. The efficient disease-resistant pralidoxime bacterial manure for the tobacco comprises adsorbent and bacterial suspension, wherein the bacterial suspension contains Pseudomonas choloraphis XQ902, and the adsorbent is grass carbon, active carbon, vermiculite or mixture thereof. In the efficient disease-resistant pralidoxime bacterial manure, 100 milliliters of bacterial suspension is added into each 400 to 600 grams of adsorbent. The method for producing the bacterial manure comprises the following steps: (1) preparing the bacterial suspension; (2) sterilizing the adsorbent; and (3) inoculating and culturing the adsorbent. The bacterial manure can increase high-grade tobacco ratio, coordinate the chemical components of the tobacco, reduce impurity gases and excitation, promote growth of tobacco roots, effectively prevent and control infestation of harmful tobacco bacteria, and improve utilization rate of soil phosphorus.

The invention discloses a saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof, and belongs to the technical field of microorganisms. The saline-alkaline tolerancephosphorus solublizing bacteria strain is named phosphorus solublizing bacteria strain RPB03, which is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No:15928, on June 11, 2018. The strain is high in adaptability to a high-salt and high-alkali environment, can increase the effective phosphorus content in calcium phosphate fermented liquid to 430 mg/L and grows well in a saline-alkaline soil environment, and indigenous microorganisms have little impact on the phosphorus solublizing effect of the saline-alkaline tolerance phosphorussolublizing bacteria strain; the effective utilization rate of phosphorus in saline-alkaline soil in a saline-alkaline farmland, a forest and the like can be effectively increased, and the plant biomass is increased. Due to the application of the strain, soil hardening caused by use of chemical fertilizer can be relieved; the utilization rate of soil phosphorus is increased; the environmental pollution is reduced.

The invention discloses a bacillus cereus for activating soil nutrient and application of the bacillus cereus. The bacillus cereus is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 21, 2012 with preservation number of CGMCC No. 6127, and the 16SrDNA nucleotide sequence is shown by SEQ ID No: 1. According to the bacillus cereus, the soil nutrient can be obviously activated, the effectiveness of phosphorus and nitrogen in soil can be improved, the composition of a soil microbial community can be improved, the diversity and the evenness of the soil microbial community can be improved, the metabolic activity of the soil microbe can be improved and the like, the soil fertility can be improved, the crop growth can be promoted, the bacillus cereus is good for the agricultural sustainable development and utilization, and the bacillus cereus is wide in application prospect in the production practice.

The invention relates to the leaching and the detection of a phosphorus component in the soil and particularly relates to a method for detecting a phosphorus component in soil by using BD-liquid 31PNMR (pulse nuclear magnetic resonance). The method comprises the following steps of: dissolving the soil to be detected by using NaOH-EDTA solution, centrifugally separating the dissolved solution to obtain soil phosphorus leach liquor, adding a sodium bicarbonate-hyposulphite buffer solution in the leach liquor, and carrying out freeze-drying treatment; and re-dissolving the powder obtained after the freezing treatment, and finally, detecting by using the 31PNMR to obtain the phosphorus component in the soil to be detected in quantitative and qualitative forms. The phosphorus in the soil is leached and detected by using a physical and chemical combined method, so that the resolution and the veracity of the 31PNMR are increased; and the method is suitable for identification and analysis of the phosphorus component in the soil.

The invention discloses a special fertilizer for orchids. The preparation method of the fertilizer comprises the following steps: when air humidity is 60-80% and temperature is 20-35 DEG C, evenly mixing 8-10 parts by weight of traditional Chinese medicine slags, 15-18 parts by weight of degenerative glucose, 3-5 parts by weight of degenerative beer liquid, 20-25 parts by weight of fallen leaf humus and 60-80 parts by weight of water; accumulating and shaping; covering with a plastic film; fermenting for 48-72 hours; then spraying 2-3 parts by weight of potassium permanganate solution for deodorization; then adding 6-8 parts by weight of soil phosphorus and potassium activator; evenly mixing; and carrying out subpackage after naturally drying. The special fertilizer provided by the invention has the advantages of abundant raw material, low cost and high fertilizer efficiency.

A phosphate-solubilizing bacterium strain, a compound bacterial agent including the same and a DEHP degrading bacterium and applications of the compound bacterial agent are disclosed. The strain is Pseudomonas sp.YLYP29 deposited in the China Center for Type Culture Collection in Wuhan University, Wuhan, China on June 28, 2017 with an accession number of CCTCC NO:M 2017391. The compound bacterialagent consists of the phosphate-solubilizing bacterium YLYP29 and the DEHP degrading bacterium XB, has good synergistic effects, high adaptive capacity, phosphate-solubilizing and DEHP degrading functions and a good application potential, and a culture method is simple and feasible. The compound bacterial agent can solve the problem of soil phosphorus deficiency, can remove DEHP pollutants in soil, improves soil nutrition states, improves soil ecological fertility and restores DEHP polluted soil, thus ensuring agricultural product quality and yield. The compound bacterial agent plays an important role in restoration of farmland soil along with production.

The invention discloses a method for determining the use quantity of a phosphate fertilizer based on available phosphorus in a land. The method comprises the following steps that step 1, according tothe soil phosphorus level, the land is divided into a phosphorus deficient area, a suitable area and a high phosphorus area, and then the return rate K of the phosphorus in the land in season is determined; step 2, the formula for obtaining the phosphorus requisite amount C and the available phosphorus soil fertility yield of different crops is YOlsen-P through long-term monitoring and research; step 3, according to different soil textures, a correction coefficient A is established; step 4, according to a wheat yield target Yt, a model IP equal to C*(Yt-YOlsen-P)*(1+K)*A is established. By establishing a phosphate fertilizer variable fertilization model and using a phosphorus big data platform, a phosphate fertilizer recommendation system based on the available phosphorus level is developed to achieve precise phosphorus application. The method has the advantages that variable phosphorus application can be carried out, the phosphorus application amount can be accurate to 0.5 kg/mu, thereby reducing the situation that the more phosphate fertilizer or less phosphate fertilizer is applied so that crops can be stably produced.

The invention discloses phosphorus-dissolving penicillium oxalicum with a broad spectrum acid production feature. The preservation number of the phosphorus-dissolving penicillium oxalicum is CCTCC No.M2019233. The phosphorus-dissolving penicillium oxalicum can grow well in a solid culture medium using calcium phosphate or iron phosphate as the only phosphorus source, the penicillium oxalicum canutilize insoluble phosphorus compounds as the growth substance after being cultured for 48 hours, and the penicillium oxalicum can grow a large number of colonies after being cultured for 3-7 days; ina liquid culture medium, the penicillium oxalicum can efficiently utilize calcium phosphate or iron phosphate with the maximum concentration being 50g/L to produce up to 2500mg/L of soluble phosphate. The penicillium oxalicum in a culture medium added with solid calcium phosphate can produce oxalic acid, formic acid, malic acid, acetic acid and lactic acid through metabolism, and the secretion quantity reaches up to 215.73mg/L. The microbial strain resource which is separated from a soil environment for the first time has a good application value in farmland soil phosphorus improving, soil fertility improving and soil heavy metal contamination comprehensive treatment promoting.

The invention belongs to the field of environmental microorganisms, and particularly relates to a production method of efficient phosphorus-dissolving epicoccum sorghinum with the characteristic of resisting heavy metals. Through classification, the epicoccum sorghinum is identified. The production method includes the steps that copper tailing soil and sterile water are cultured in a mixed mode, and a heavy metal sterile mixed solution with the concentration suitable for the growth of thalluses is screened; repeated culture is conducted for six times; fourth-time culture liquid, fifth-time culture liquid and sixth-time culture liquid are sucked and added into culture mediums containing organic phosphorus and inorganic phosphorus for plate spread culture, culture dishes with a large numberof bacterial colonies are selected, plate streaking culture is conducted in the culture mediums containing the organic phosphorus and the inorganic phosphorus, and the bacterial colonies with obviousphosphorus-dissolving rings are screened; and culture purification results are subjected to shake cultivation, and finally screening purification is conducted to obtain phosphorus-dissolving bacterialstrains resisting the heavy metals. Efficient phosphorus-dissolving funguses resisting the heavy metals are screened from contaminated land, the soil phosphorus supply and demand contradiction can beadjusted, reclamation land soil in a mining area is improved, vegetation growth is promoted, the utilization ratio of heavy metal contaminated plowland soil phosphorus by crops is increased, and useof fertilizer is reduced.

The invention relates to multi-functional arthrobacter Fp64 for controlling soil phosphorus loss and application thereof. The arthrobacter Fp64 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 26, 2018, and the preservation number is CGMCC No.15644. The arthrobacter Fp64 is used as a phosphorus regulating agent or the like in agricultural production, hasgood environmental adaptability and has the ability to resist pathogenic bacteria. When the arthrobacter Fp64 is added to soil, the loss of phosphorus in the soil can be effectively prevented.

The invention provides application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation, relates to the technical field of microbial pesticides, and particularly provides application of bacillus subtilis XF-1 in the soil phosphorus decomposition and the cellulose degradation. The bacillus subtilis XF-1 has remarkable phosphorus decomposing and cellulose degrading functions, can tolerate a certain degree of salinity and can survive in a 2.5% of sodium chloride solution, and accordingly a bacillus subtilis XF-1 fungicide can be used in salinity soil.

The invention relates to a soil phosphorus activator and a preparation method therefor. The soil phosphorus activator is prepared from urea phosphate, fulvic acid, sodium alginate, oxalic acid, citric acid, maleic acid and compound sodium nitrophenolate. The activator is prepared by the steps: crushing the raw materials by a crusher; screening the crushed materials by a 150-mesh sieve; and after uniformly mixing the powder in proportion, bagging and packaging the powder to obtain the activator. The soil phosphorus activator can be used for activating fixed phosphorus in soil, and has functions of promoting crops to absorb phosphorus, improving the yield of the crops and increasing the content of rapid available phosphorus, and has a relatively good effect on reduction of use of a phosphorus fertilizer; moreover, the growth of the crop root system can be effectively promoted to improve the root system activity so as to improve the absorption capacity of the root system on soil phosphorus, thereby indirectly improving the yield of the crops; the water solubleness of the activator is greater than 99.99% and the soil phosphorus activator can be applied as a trickle irrigation fertilizer.

The invention relates to a calcareous soil phosphorus activator and a preparation method and application thereof. The calcareous soil phosphorus activator comprises active ingredients including crop straws, coal ash, bentonite, humic acid, seaweed extract, organic acid, amino acid, phytase and the like. The calcareous soil phosphorus activator has the advantages that through physical and chemicalactions, phosphorus fixation of calcareous soil can be alleviated effectively, phosphorus fixed in the soil can be activated, unavailable phosphorus in the soil can be facilitated to be converted intoavailable phosphorus, and accordingly the calcareous soil phosphorus activator is widely applicable to the calcareous soil in different areas; in a process of activating a soil phosphorus nutrient after being applied into the soil, the calcareous soil phosphorus activator can also facilitate soil phosphorus nutrient absorbing of crops, can enhance fertilizer efficiency of a fertilizer when beingapplied together with the fertilizer, and can increase output and quality of the crops.

The invention relates to soil-phosphorus biological-activating fungi inoculant and a preparation method thereof; the preparation method comprises the following steps: taking a slant strain, cultivating the slant strain at 25 degrees centigrade for 1-2 days; eluting spores with 15mL of sterile water so as to prepare spore suspension, absorbing 10mL of spore suspension, inoculating the spore suspension into a conical flask containing 100mL of PD (potato dextrose) culture solution, carrying out shaking culture at 26 degrees centigrade for 3-5 days so as to obtain a liquid strain; inoculating the liquid strain in an amount of 15-20% into first-grade seed PD culture solution; carrying out cultivation at 27 degrees centigrade for 1-2 days; inoculating the first-grade seed PD culture solution in an amount of 15-20% into second-grade seed PD culture solution so as to obtain a second-grade liquid strain; and absorbing the second-liquid strain with sterile grass carbon according to a ratio of 1: 4, then, crushing so as to obtain spore powder.

The invention provides a pseudomonas strain, which is named P623-9 and has been currently preserved in Guangdong Microbial Culture Collection Center (GDMCC). The address is Floor 5, NO.59 Building, No.100 Yard, Xianlie Middle Road, Guangzhou, the postal code is 510070, the preservation number is GDMCC NO.60806, and the preservation date is October 14, 2019. The invention also discloses applicationof the pseudomonas in improving the phosphorus utilization rate of greenhouse vegetable soil and promoting growth. According to the pseudomonas, the phosphorus dissolving amount of P623-9 is as highas 496 mg/L; the pseudomonas has good phosphorus dissolving capacity, can convert insoluble phosphatephosphorus such as fixed-state phosphorus in soil into soluble phosphorus which is easily absorbedand utilized by vegetables, reduces the application amount of a phosphate fertilizer, promotes the growth of vegetables, increases the yield by 15% or above in the application process, saves the planting cost and relieves the pollution problem.

The invention relates to especially relates to an activator for soil phosphorus, belonging to the technical field of activation of phosphorus. The activator for soil phosphorus comprises, by weight, 15 to 25 parts of phosphorus-dissolving microbes, 70 to 80 parts of a carrier and 2 to 8 parts of a protective agent, wherein the protective agent is one or a mixture of more than one selected from a group consisting of bone meal, gelatin, citric acid and amino acid. The invention also provides a preparation method and application of the activator for soil phosphorus. The activator for soil phosphorus in the invention can activate slightly-soluble phosphorus in soil, improve the state of phosphorus in soil and help plants in synergistic absorption of nitrogen, phosphorus and potassium, so the application amount of fertilizer is reduced, the efficiency of fertilizer is improved, and the activator exerts good ecological effect on energy conservation and environmental protection.

The invention relates to a preparation method of an exogenous easily extractable glomalin soil conditioner. The method comprises the steps of: (1) preparation of a sodium citrate extraction agent: mixing a citric acid solution with a disodium hydrogen phosphate solution in a ratio of 13:87 evenly to obtain the sodium citrate extraction agent; (2) preparation of the exogenous easily extractable glomalin soil conditioner: adding air-dried soil into the sodium citrate extraction agent, then conducting sterilization and centrifugation, and collecting the supernate, thus obtaining an exogenous extracted glomalin solution; and (3) dilution of the solution prepared by step (2) with a sodium citrate buffer solution, thus obtaining the exogenous easily extractable glomalin soil conditioner. The exogenous easily extractable glomalin soil conditioner prepared by the method provided by the invention increases the plant rhizosphere endogenous soil glomalin content, promotes plant vegetative growth and formation of plant rhizosphere water-stable macro-aggregates, improves the soil structure, improves the activity of peroxidase, polyphenol oxidase and soil phosphatase in plant rhizosphere soil, and promotes soil nutrient circulation. The preparation method has the advantages of simple process, safety and high efficiency, green and no pollution, and significant ecological and economic benefits.