44 results about "Soil phosphorus" patented technology

The invention relates to the technical field of agricultural production, and discloses a salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 and application thereof in corn planting, the salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 is a purple blue bacterium, the preservation number of the strain is CGMCC No.42313, and the strain has multiple functions of resisting salt and alkali, dissolving insoluble phosphorus and degrading 2-methyl-4-chlorophenoxyacetic acid pesticide residues. The strain, a culture thereof or a microbial inoculum prepared from the strain are applied to agricultural production, and plant growth is promoted by exerting the composite biological function of the strain under saline-alkali stress. According to the invention, the synergistic effect of saline-alkaline tolerance, phosphate solubilization and pesticide degradation of the strain is utilized, so that the plant growth inhibition is relieved in the adverse situation, the soil phosphorus bioavailability is improved, the pesticide-polluted soil is repaired, and the plant seed germination, root development and plant growth are promoted. The strain has an important application prospect in the aspect of developing a microbial agent for saline-alkali soil improvement.

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention belongs to the field of microorganisms, and relates to a phosphate-solubilizing fungus with sandy soil adaptability, in particular to a high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain as well as a screening method and application of the high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain. The talaromyces sp. HZ29 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.42246, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on October 15, 2025. A new phosphorus-solubilizing fungus strain is obtained through screening for the first time, phosphorus which is difficult to be directly absorbed by crops in soil can be efficiently activated, the content of available phosphorus in the soil is increased, and the phosphorus-solubilizing fungus strain has an outstanding phosphorus-solubilizing effect; and the strain has siderophore secretion capability and disease bacterium antagonism capability, and has a growth promoting function on crops.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a composite solid phosphorus-solubilizing bacterial agent for repairing cadmium-polluted soil and improving soil quality, wherein the composite solid phosphorus-solubilizing bacterial agent comprises phosphorus-solubilizing bacteria, bone charcoal, culture medium and organic matrix; the phosphorus-solubilizing bacteria are Pantoea agglomerans CT-3 and Enterobacter hormaechei HS-6; the composite solid phosphorus-solubilizing bacterial agent is applied to Cd-polluted soil, and the biological availability of Cd in the soil is reduced by Pantoea agglomerans CT-3, Enterobacter hormaechei HS-6 and micron-nanometer multi-stage bone charcoal, and meanwhile, the soil quality is improved. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of effective soil phosphorus is increased by 98.19%; on the other hand, the application can fix soil heavy metal Cd, so that the content of effective Cd in the soil is reduced by about 52.50%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in repairing farmland Cd pollution, improving soil quality and environmental protection.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

The invention is applicable to the technical field of soil improvement, provides a method for improving soil by inputting organic materials and application, and aims to solve the degradation problems of soil hardening and nutrient imbalance of Yuluihu dry-hot valley vineyards in the method, cow dung, fungus bags and mixtures of the cow dung and the fungus bags are used as organic materials, and random block test design is adopted. The method comprises the following steps: dividing a degraded vineyard into plots and test plots with specific specifications, applying organic materials, and measuring the water retention capacity, aggregate composition and nutrient element content of soil through layered soil sampling to realize soil quality regulation and control. Adaptive organic materials can be selected according to soil problems and grape growth requirements, the cow dung can remarkably improve the phosphorus content of the soil, the cow dung and the fungus bags can optimize the soil aggregate structure, the water and fertilizer retention capacity, ventilation and water permeability of the soil are effectively improved, technical support is provided for sustainable development of the dry-hot valley grape industry, and the application prospect is wide.

The invention discloses a comprehensive nutrition regulation and control method for improving overwintering cold resistance of citrus fruit trees, and relates to the field of fruit tree cultivation, and the method comprises the following steps: S1, soil conditioning and mycorrhiza inoculation after fruit picking: determining the content of rapidly available phosphorus in orchard soil, when the content of rapidly available phosphorus is greater than 30mg / kg, firstly applying humic acid and calcium magnesium phosphate fertilizer for phosphorus reduction treatment, and after the content of rapidly available phosphorus is reduced to be less than or equal to 20mg / kg, then applying a nutrient solution; digging radial short ditches on the inner side of a water drip of a tree crown, mixing a pre-activated arbuscular mycorrhizal fungus inoculant with an organic carrier, filling the mixture into the ditches, and earthing and watering; according to the comprehensive nutrition regulation and control method for improving the overwintering cold resistance of the citrus fruit trees, through soil phosphorus pre-regulation and local enrichment inoculation methods, the mycorrhizal fungus colonization rate is increased to 50% or above from less than 20%, and the mycorrhizal inoculation effect and the input-output ratio are remarkably improved.

The invention discloses a method for preparing slow-release phosphate fertilizer soil from graphite tailing sand and coal gangue and application, and relates to the technical field of solid waste resource utilization and agricultural fertilizer. The slow-release phosphate fertilizer soil is prepared by carrying out gradient magnetic separation-acid leaching synergistic pretreatment on graphite tailing sand (the removal rate of magnetic impurities reaches 92%-95%), carrying out segmented roasting-microwave activation treatment on coal gangue (the specific surface area is increased to 28.6-32.4 m < 2 > / g), then mixing the pretreated raw materials with a composite exciting agent, and carrying out a low-temperature sintering-granulation molding process. The problem of low resource utilization rate of the graphite tailing sand and the coal gangue is effectively solved, the soil phosphorus slow release period of the prepared phosphate fertilizer reaches 120-150 days, the prepared phosphate fertilizer can meet the nutrient requirement of phosphorus-loving crops in the whole growth period, compared with a traditional phosphate fertilizer, the usage amount is reduced by 30-40%, the phosphorus utilization rate is increased to 65-72%, and remarkable economic, environmental and social benefits are achieved.

This invention belongs to the field of agricultural biotechnology, specifically relating to the application of a compound biological agent in agriculture. The compound biological agent comprises live engineered Bacillus subtilis bacteria and a 3% glucose solution; wherein the engineered Bacillus subtilis bacteria are obtained through genetic modification using Bacillus subtilis as the substrate bacteria, and the live bacteria concentration is ≥1×10¹. 0 The 3% glucose solution (CFU / mL) is a 3% (w / v) aqueous solution of glucose, and the two are mixed at a volume ratio of 1:(800-1200). This invention's compound biological agent combines the soil microecological regulation and root growth promotion capabilities of Bacillus subtilis engineered live bacteria with the sugar metabolism regulation effects of 3% glucose. The synergistic effect of these two agents significantly promotes beet root growth, increases the activity of beet sugar metabolism enzymes, and increases the accumulation of sucrose and soluble sugars in the tubers. Simultaneously, it improves the rhizosphere soil microecological environment of beet and enhances soil phosphorus activation capacity. This provides an environmentally friendly and efficient biological agent for high-quality and high-yield beet cultivation, and has promising agricultural application prospects.

The invention discloses a phosphate-solubilizing complex microbial inoculant suitable for medium and low-yield field soil and application thereof, and belongs to the technical field of microbial inoculants. The phosphate-solubilizing composite bacterial agent is prepared by mixing the following components according to a ratio of 1: 1: 1: 1: 1: (Stennotrophomonas sp. Strain nsu-56), Klebsiella pseudomonas sp. Strain KPNORMBLD13757, Sphingomonas sp. Strain B12 and Bacillus amyloliquefaciens strain LZ12-01, wherein the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, so that the phosphate-solubilizing composite bacterial agent can be used for preparing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent. The effective viable count of the prepared phosphate-solubilizing complex microbial inoculant is 1.4 * 10 < 9 >-1.6 * 10 < 9 > cfu / ml. The invention also provides a preparation method of the microbial inoculum, the microbial inoculum is subjected to expanding culture for 12-18 hours through a shaking table at 25-35 DEG C and 160-200r / min during preparation, and an embedding carrier material can be further adopted for embedding treatment to improve the stress resistance. The microbial agent is sprayed on the surface of soil, the dosage is 1 L / mu, soil phosphorus can be efficiently activated, crop growth is promoted, technical support is provided for soil improvement of medium and low-yield fields and crop yield increase, and the application prospect is wide.

The invention relates to the technical field of soil improvement effect evaluation, and discloses a rapid evaluation system and method for a microbial soil improvement effect, and the method comprises the following steps: preparing a test soil system containing a saline-alkali soil indigenous microbial community; setting a test group into which the microbial soil conditioner is introduced and a control group into which the conditioner is not introduced; applying a preset saline-alkali stress condition to trigger the reconstruction of the microbial function network; monitoring and comparing microbial function network metabolic flux of the test group and the control group during stress, wherein the microbial function network metabolic flux comprises a soil nitrogen conversion function network and a soil phosphorus activation function network; on the basis of the improvement degree of the metabolic flux of the test group relative to the control group, the ability of the modifier to reconstruct the microbial function network is characterized, and the ability is positively correlated with the field long-term improvement effect. The system and the method realize short evaluation period of the microbial soil improvement effect and accurate and reliable result, and are suitable for efficient screening, application and popularization of the microbial soil conditioner.

The invention discloses a preparation method and application of a calcium-phosphorus-loaded hydrothermal humic carbon-based soda saline-alkali soil slow-release conditioner, and belongs to the technical field of soda saline-alkali soil remediation and fertilizer. The invention aims to solve the problems that a modifier used in an existing soda saline-alkali soil improvement technology is short in validity period and inconvenient to transport and apply, possibly causes secondary environmental risks and cannot synergistically and continuously provide nutrients such as phosphorus for soil. The method comprises the following steps: 1, preparing phosphorus-rich hydrothermal humic carbon; 2, preparing sepiolite modified phosphorus-rich hydrothermal humic carbon; and 3, preparing a cross-linked protective layer. The invention breaks through the limitation of single function of the traditional saline-alkali soil remediation technology, develops the soda saline-alkali soil conditioner which is green, low in cost, easy to apply and high in stability and integrates multiple environment responses of high Na < + >, high pH and phosphorus starvation, and solves the problem of phosphorus starvation of the remediated soil while reducing the harm of soil salinization. And a green and sustainable solution is provided for treatment of soda saline-alkali soil.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The application belongs to the field of microorganisms, and relates to a phosphorus solubilizing fungus with sandy soil adaptability, in particular to a high-efficiency sandy soil phosphorus solubilizing Talaromyces HZ29 and a screening method and application thereof. Talaromyces pseudofuniculosus The preservation number of the Talaromyces HZ29 is CGMCC NO.42246, and the Talaromyces HZ29 is preserved in the China General Microbiological Culture Collection Center on October 15, 2025. The application first screens a new phosphorus solubilizing fungus strain, can efficiently activate the phosphorus in soil which is difficult to be directly absorbed by crops, improves the content of effective phosphorus in soil, has an outstanding phosphorus solubilizing effect, and has the abilities of iron carrier secretion and antagonism to plant disease bacteria and the function of promoting crop growth.

The invention relates to the technical field of microorganisms, and discloses pantoea H1 and a demonstration method and application thereof in agriculture assistance, the pantoea H1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36875. The invention further provides a microbial agent containing the bacterial strain, a preparation method and application of the bacterial strain in crop planting, the bacterial strain has efficient dual phosphorus solubilizing capacity, indissolvable inorganic phosphorus can be dissolved, organophosphorus can be mineralized, and the effectiveness of soil phosphorus is improved; meanwhile, the microbial agent has excellent saline-alkaline tolerance, can still keep high activity under adversity, and by applying the microbial agent in the seedling stage of crops, the rhizosphere microenvironment can be remarkably improved, root development and plant growth of the crops such as corn and mung beans can be promoted, the emergence rate and yield of the crops in soil such as saline-alkaline land can be increased, and the crop yield can be increased. And an excellent microbial germplasm resource is provided for activating soil nutrients, reducing weight and improving efficiency.

The application discloses a biodegradable coated urea added with zirconium-based MOF nanocrystals with phosphatase activity and a preparation method and application thereof, and belongs to the technical field of fertilizers. The zirconium-based MOF nanocrystals with phosphatase activity are prepared, a solution of the zirconium-based MOF nanocrystals is mixed with urea and dried, polypropylene carbonate is used as a coating material, and then the biodegradable coated urea added with the zirconium-based MOF nanocrystals with phosphatase activity is prepared through a fluidized bed solvent method. The coated urea prepared in the application can realize nitrogen control release, hydrolyze soil organic phosphorus through the phosphatase activity of the Zr-MOF nanocrystals, realize the dual functions of continuous supply of nitrogen and in-situ activation of soil phosphorus, simultaneously meet the demand of crops for two core nutrient elements of nitrogen and phosphorus, significantly relieve the low-phosphorus stress of crops, promote the growth of the root system and the aboveground part of crops, and improve the biomass of crops.

This application discloses a method and system for applying microbial fertilizer to promote phosphorus absorption in alfalfa. The method includes: determining the amount of microbial fertilizer to be applied to each alfalfa area based on the plant phosphorus content, total soil phosphorus content, and available soil phosphorus content; activating sensor equipment pre-installed in the alfalfa field to acquire alfalfa growth environment information and calculating the daily irrigation amount for the alfalfa field using the growth environment information; preparing a fertilizer solution for a drip irrigation fertilization system based on the microbial fertilizer application amount and the daily irrigation amount, and using the fertilizer solution to perform irrigation fertilization on the alfalfa field to complete the application of microbial fertilizer to promote phosphorus absorption in alfalfa. Through the solution of this application, the dissolution and release of phosphorus in the soil can be promoted, thereby improving the phosphorus absorption efficiency of alfalfa.

The application discloses a kind of algorithm based on quantification crop rhizosphere soil phosphorus biological activation turnover quantity determines the phosphorus fertilizer application amount;Including: determine the phosphorus requirement of crop target yield;Determine the activation turnover flux of crop rhizosphere microbial quantity phosphorus;Determine the phosphorus fertilizer availability loss rate of fertilization plot soil;Establish quantitative phosphorus application algorithm.The recommended amount of phosphorus fertilizer calculated by the quantitative phosphorus application algorithm provided by the application is reduced compared with the amount recommended by the constant monitoring technology based on soil available phosphorus level under the premise of ensuring crop yield, so that the fertilizer phosphorus input of farmland and the amount of crop taken away are basically balanced.

The invention discloses a soil remediation agent and application thereof, and belongs to the technical field of soil remediation. The soil remediation agent comprises micro-nano bone black and a penicillium fistulosum solid fungicide. Penicillium fimbriatum can convert insoluble phosphorus in soil into available phosphorus and increase phosphorus nutrition of the soil, micro-nano bone black can improve cation exchange capacity of the soil and enhance water and fertilizer retention capacity of the soil, and soil fertility can be comprehensively improved by combining the two. The phosphorus-solubilizing fungus and micro-nano bone black combined remediation technology is adopted, dissolution of insoluble phosphate can be effectively promoted, and the content of available phosphorus in soil is increased by 2.69 times; the soil heavy metal cadmium (Cd) can be fixed, the effective state Cd of the soil is reduced by about 25.91%, and the soil heavy metal cadmium (Cd) fixing agent has high Cd fixing capacity and has good application value in farmland Cd pollution remediation and environmental protection. The soil remediation agent provided by the invention not only can realize efficient remediation of cadmium-contaminated soil, but also is green and environment-friendly, and is suitable for large-scale popularization and application.

The invention discloses a polygonum cuspidatum root extract modified microbial agent and a preparation method thereof, and relates to the technical field of microbial preparations. According to the microbial inoculum, alpha-L-rhamnosidase and beta-glucosidase are cooperated, the hydrolysis degree of polydatin is accurately controlled to be 32%-38%, and 2%-3% of polygonum cuspidatum polysaccharide is synchronously added to stabilize the enzyme activity. The hydrolysate is matched with bacillus subtilis and trichoderma harzianum composite flora, so that the phosphate-solubilizing enzyme secretion amount of the bacillus subtilis and trichoderma harzianum composite flora is increased by 50%-65%, the pathogenic bacterium inhibition rate of the trichoderma harzianum composite flora is increased by 40%-52%, and the flora survival time is prolonged to 60 days. After application, the crop disease control rate reaches 78%, and the soil available phosphorus content is increased by 62%. The method solves the problems of antagonism of complex flora and easy inactivation of enzyme activity, and is simple in process, easy to industrialize and suitable for planting of vegetables and traditional Chinese medicinal materials.

The invention provides a preparation method and application of a black talc loaded hydrotalcite functional material, and belongs to the technical field of black talc utilization. Black talc is mechanically ground and sieved, black talc powder is added into a mixed solution of divalent metal ions and trivalent metal ions, alkali liquor is added, hydrotalcite is loaded on the black talc through a coprecipitation method, the black talc-hydrotalcite composite material is constructed, and phosphorus in water is efficiently removed. Meanwhile, the adsorbed material is used as a soil phosphate fertilizer, a silicon fertilizer and a heavy metal repairing agent.

The invention relates to the technical field of microbial agents. The invention provides a microbial agent. The microbial agent is prepared from the following components: 14 to 18 parts of bacillus megaterium, 5 to 10 parts of bacillus coagulans, 8 to 13 parts of penicillium baylii fungi, 6 to 11 parts of penicillium oxalicum fungi, 3 to 5 parts of arbuscular mycorrhizal fungi, 2 to 6 parts of bacillus mucilaginosus, 7 to 10 parts of aspergillus niger and 4 to 6 parts of paecilomyces lilacinus. According to the microbial agent disclosed by the invention, various bacteria are matched for use, so that phosphides which cannot be utilized by crops in soil can be effectively promoted to be converted into soluble phosphides which can be utilized, the content of available phosphorus in the soil is increased, the effectiveness of phosphorus is improved, absorption and utilization of the crops are ensured, and the growth and development of the crops are promoted.

The invention discloses a carbon-based bio-organic fertilizer with phosphorus immobilization and biological activation functions and application, and belongs to the technical field of agricultural environments and novel fertilizers. The organic fertilizer is prepared by compounding a high-performance phosphorus adsorption carbon-based material, a low-phosphorus leaching-loss organic fertilizer carrier, a composite functional microbial agent and fulvic acid, wherein the carbon-based material is fir sawdust carbon or rice hull carbon with the specific surface area being larger than or equal to 19 m < 2 > / g and the micropore proportion being larger than or equal to 60%, and the organic fertilizer carrier is decomposed sheep manure organic fertilizer; the microbial agent is prepared from trichoderma viride Tv41 and trichoderma longibrachiatum TB2; according to the organic fertilizer, a'carbon-fertilizer-bacterium 'ternary synergistic system is constructed, so that the bio-availability of the organic fertilizer is enhanced while soil phosphorus is effectively immobilized, the problem that immobilization and activation are difficult to cooperate in the traditional technology is solved, phosphorus loss is synchronously controlled and the absorption and utilization efficiency of crops on phosphorus is improved under the condition of reducing the dosage of a phosphate fertilizer, and the yield of the crops is increased. The method is suitable for application of crops such as cabbages and hot peppers in phosphorus-enriched vegetable fields.

The invention discloses preparation of benzyl dipeptide and application of the benzyl dipeptide in agriculture, and relates to the field of application of the benzyl dipeptide in agriculture, the preparation of the benzyl dipeptide and the application of the benzyl dipeptide in agriculture remarkably improve the utilization rate of phosphate fertilizer by preparing the benzyl dipeptide and applying the benzyl dipeptide in agriculture. Benzyl dipeptide is added into soil with different phosphorus levels, the phosphorus accumulation amount and the phosphorus utilization rate of crops are greatly increased, and particularly, the effect is the best when the phosphorus application amount is 30 mg / kg and 20 ng / mL of benzyl dipeptide is added. The method reduces fixation and loss of a phosphate fertilizer in soil, enables more phosphorus elements to be absorbed and utilized by crops, reduces waste of the phosphate fertilizer, relieves the pressure of excessive phosphate fertilizer on the environment, realizes green and sustainable development of agricultural production, screens out the optimal use concentration of the benzyl dipeptide through system tests, is applied to different crops, and has a wide application prospect. Growth indexes such as crop plant height and overground fresh weight are obviously improved.

The application discloses a kind of phytase-degrading phosphorus microbial compound microbial inoculant, preparation method and application, the microbial compound microbial inoculant includes: phytase-degrading phosphorus promoting growth bacteria Pseudomonas mandelii (GAU-00663), Pseudomonas baetica (GAU-00664) and Pseudomonas simiae (GAU-00690) strain culture solution.The microbial compound microbial inoculant provided in the application contains drought-tolerant nitrogen-fixing, phosphorus-degrading and plant hormone-secreting strains, and thus has the functions of phosphorus-degrading, drought-tolerant, growth-promoting and soil-improving, can improve soil phosphorus utilization efficiency, improve soil enzyme activity, improve soil fertility and promote plant growth, is convenient to operate, high in benefit, can be seed-dressing, drip irrigation, and can also be directly sprayed.

The invention belongs to the technical field of microorganisms, and particularly relates to drought-enduring phosphorus-solubilizing pantoea CYIJM06 and application thereof. The invention provides pantoea CYIJM06, the preservation number is CCTCC NO: M 20222103, the pantoea CYIJM06 is drought-resistant and strong in phosphate solubilizing capacity, and the pantoea CYIJM06 is prepared into a fungicide for application and can improve soil, activate soil phosphorus and increase the content of available phosphorus in the soil. The Pantoea sp. CYIJM06 provided by the invention is especially suitable for activation of phosphorus in soil in arid and / or semi-arid regions, the content of rapidly available phosphorus in the soil in the arid and / or semi-arid regions can be remarkably increased, and application of phosphate fertilizer is reduced. The phosphorus-solubilizing strain provided by the invention not only can release micromolecular activated fixed inorganic phosphorus such as organic acid in a culture medium, but also can secrete phosphatase in the culture medium to hydrolyze organic phosphorus, so that the phosphorus activation of soil is obviously promoted.

The present invention provides Paraburkholderia sp. LSOJM18 and use thereof as well as a microbial inoculum, belonging to the technical field of microorganisms. The Paraburkholderia sp. LSOJM18 was deposited to the China Center for Type Culture Collection on Dec. 29, 2022, with the depository number CCTCCNO: M20222102. With a strong phosphate solubilization capacity, the strain provided by the present invention is suitable for activating phosphorus in farmland soil. Using the phosphate-solubilizing bacteria can significantly increase the available phosphorus content of soil and reduce the application of chemical phosphate fertilizers. Acting on a wide range of substrates, the phosphate-solubilizing bacteria strain provided by the present invention can not only secrete phosphatase to hydrolyze organic phosphorus, but also release small molecules such as organic acids, etc., to activate the fixed inorganic phosphorus, and using the phosphate-solubilizing bacteria can significantly improve the phosphorus activation capacity of soil.