70 results about "Soil phosphorus" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention relates to the technical field of agricultural production, and discloses a salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 and application thereof in corn planting, the salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 is a purple blue bacterium, the preservation number of the strain is CGMCC No.42313, and the strain has multiple functions of resisting salt and alkali, dissolving insoluble phosphorus and degrading 2-methyl-4-chlorophenoxyacetic acid pesticide residues. The strain, a culture thereof or a microbial inoculum prepared from the strain are applied to agricultural production, and plant growth is promoted by exerting the composite biological function of the strain under saline-alkali stress. According to the invention, the synergistic effect of saline-alkaline tolerance, phosphate solubilization and pesticide degradation of the strain is utilized, so that the plant growth inhibition is relieved in the adverse situation, the soil phosphorus bioavailability is improved, the pesticide-polluted soil is repaired, and the plant seed germination, root development and plant growth are promoted. The strain has an important application prospect in the aspect of developing a microbial agent for saline-alkali soil improvement.

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention belongs to the field of microorganisms, and relates to a phosphate-solubilizing fungus with sandy soil adaptability, in particular to a high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain as well as a screening method and application of the high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain. The talaromyces sp. HZ29 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.42246, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on October 15, 2025. A new phosphorus-solubilizing fungus strain is obtained through screening for the first time, phosphorus which is difficult to be directly absorbed by crops in soil can be efficiently activated, the content of available phosphorus in the soil is increased, and the phosphorus-solubilizing fungus strain has an outstanding phosphorus-solubilizing effect; and the strain has siderophore secretion capability and disease bacterium antagonism capability, and has a growth promoting function on crops.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a composite solid phosphorus-solubilizing bacterial agent for repairing cadmium-polluted soil and improving soil quality, wherein the composite solid phosphorus-solubilizing bacterial agent comprises phosphorus-solubilizing bacteria, bone charcoal, culture medium and organic matrix; the phosphorus-solubilizing bacteria are Pantoea agglomerans CT-3 and Enterobacter hormaechei HS-6; the composite solid phosphorus-solubilizing bacterial agent is applied to Cd-polluted soil, and the biological availability of Cd in the soil is reduced by Pantoea agglomerans CT-3, Enterobacter hormaechei HS-6 and micron-nanometer multi-stage bone charcoal, and meanwhile, the soil quality is improved. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of effective soil phosphorus is increased by 98.19%; on the other hand, the application can fix soil heavy metal Cd, so that the content of effective Cd in the soil is reduced by about 52.50%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in repairing farmland Cd pollution, improving soil quality and environmental protection.

The invention discloses a high-organic-matter biological matrix and a preparation method thereof. The biological matrix is prepared from the following components in percentage by mass: 20% of imported peat, 30% of coco coir, 30% of a fermented material consisting of tobacco powder and livestock and poultry manure, and 20% of perlite. The preparation method comprises the following steps: uniformly mixing tobacco powder, livestock and poultry manure, imported peat and coco coir, composting and fermenting for not less than 45 days, controlling the temperature not to exceed 72 DEG C, crushing, screening, storing and cooling, adding microbial strains, and carrying out anaerobic fermentation in a packaging bag for 7 days; the organic matter content of the obtained biological matrix exceeds 45%, and the biological matrix can effectively loosen soil, promote crop root growth and release phosphorus and potassium elements in soil, can be used for field crop and vegetable seedling raising and the like, and has the advantages of being safe, environmentally friendly and remarkable in soil improvement effect.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

The invention is applicable to the technical field of soil improvement, provides a method for improving soil by inputting organic materials and application, and aims to solve the degradation problems of soil hardening and nutrient imbalance of Yuluihu dry-hot valley vineyards in the method, cow dung, fungus bags and mixtures of the cow dung and the fungus bags are used as organic materials, and random block test design is adopted. The method comprises the following steps: dividing a degraded vineyard into plots and test plots with specific specifications, applying organic materials, and measuring the water retention capacity, aggregate composition and nutrient element content of soil through layered soil sampling to realize soil quality regulation and control. Adaptive organic materials can be selected according to soil problems and grape growth requirements, the cow dung can remarkably improve the phosphorus content of the soil, the cow dung and the fungus bags can optimize the soil aggregate structure, the water and fertilizer retention capacity, ventilation and water permeability of the soil are effectively improved, technical support is provided for sustainable development of the dry-hot valley grape industry, and the application prospect is wide.

The invention discloses a comprehensive nutrition regulation and control method for improving overwintering cold resistance of citrus fruit trees, and relates to the field of fruit tree cultivation, and the method comprises the following steps: S1, soil conditioning and mycorrhiza inoculation after fruit picking: determining the content of rapidly available phosphorus in orchard soil, when the content of rapidly available phosphorus is greater than 30mg / kg, firstly applying humic acid and calcium magnesium phosphate fertilizer for phosphorus reduction treatment, and after the content of rapidly available phosphorus is reduced to be less than or equal to 20mg / kg, then applying a nutrient solution; digging radial short ditches on the inner side of a water drip of a tree crown, mixing a pre-activated arbuscular mycorrhizal fungus inoculant with an organic carrier, filling the mixture into the ditches, and earthing and watering; according to the comprehensive nutrition regulation and control method for improving the overwintering cold resistance of the citrus fruit trees, through soil phosphorus pre-regulation and local enrichment inoculation methods, the mycorrhizal fungus colonization rate is increased to 50% or above from less than 20%, and the mycorrhizal inoculation effect and the input-output ratio are remarkably improved.

The invention discloses a method for preparing slow-release phosphate fertilizer soil from graphite tailing sand and coal gangue and application, and relates to the technical field of solid waste resource utilization and agricultural fertilizer. The slow-release phosphate fertilizer soil is prepared by carrying out gradient magnetic separation-acid leaching synergistic pretreatment on graphite tailing sand (the removal rate of magnetic impurities reaches 92%-95%), carrying out segmented roasting-microwave activation treatment on coal gangue (the specific surface area is increased to 28.6-32.4 m < 2 > / g), then mixing the pretreated raw materials with a composite exciting agent, and carrying out a low-temperature sintering-granulation molding process. The problem of low resource utilization rate of the graphite tailing sand and the coal gangue is effectively solved, the soil phosphorus slow release period of the prepared phosphate fertilizer reaches 120-150 days, the prepared phosphate fertilizer can meet the nutrient requirement of phosphorus-loving crops in the whole growth period, compared with a traditional phosphate fertilizer, the usage amount is reduced by 30-40%, the phosphorus utilization rate is increased to 65-72%, and remarkable economic, environmental and social benefits are achieved.

The invention discloses subterranean intergeneric bacillus 52-6 with a phosphate solubilizing function and application thereof, and belongs to the technical field of microorganisms. The invention provides subterranean intergeneric bacillus, which is classified and named as subterranean intergeneric bacillus, and the preservation number of the subterranean intergeneric bacillus is CGMCC (China General Microbiological Culture Collection Center) No.32719. The subterranean intergeneric bacillus is named as subterranean intergeneric bacillus. The bacterial colony of the subterranean intergeneric bacillus 52-6 is round, light coral red, semitransparent, flat in surface and tidy in edge, the suitable growth temperature is 35-38 DEG C, the delay phase is short, the growth is rapid, and the culture is easy; the subterranean intergeneric bacillus 52-6 can efficiently dissolve phosphate insoluble in soil and promote absorption of plants to soil phosphorus elements, has the effect of promoting plant growth, effectively improves soil fertility and reduces use of chemical fertilizers, and has huge potential in application of promoting plant growth and improving soil fertility.

The application discloses application of combined inoculation of rhizosphere phosphorus solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms.The combined inoculation of acinetobacter and pantoea provided by the application can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, and promotes the utilization of phosphorus.The combined inoculation of acinetobacter and pantoea has significant soil phosphorus solubilizing capacity and plant growth promotion effect when the ratio of acinetobacter to pantoea is (4-6):(6-4), and can be applied to improving soil phosphorus utilization rate and promoting plant growth, and has important agricultural and environmental values.

This invention belongs to the field of agricultural biotechnology, specifically relating to the application of a compound biological agent in agriculture. The compound biological agent comprises live engineered Bacillus subtilis bacteria and a 3% glucose solution; wherein the engineered Bacillus subtilis bacteria are obtained through genetic modification using Bacillus subtilis as the substrate bacteria, and the live bacteria concentration is ≥1×10¹. 0 The 3% glucose solution (CFU / mL) is a 3% (w / v) aqueous solution of glucose, and the two are mixed at a volume ratio of 1:(800-1200). This invention's compound biological agent combines the soil microecological regulation and root growth promotion capabilities of Bacillus subtilis engineered live bacteria with the sugar metabolism regulation effects of 3% glucose. The synergistic effect of these two agents significantly promotes beet root growth, increases the activity of beet sugar metabolism enzymes, and increases the accumulation of sucrose and soluble sugars in the tubers. Simultaneously, it improves the rhizosphere soil microecological environment of beet and enhances soil phosphorus activation capacity. This provides an environmentally friendly and efficient biological agent for high-quality and high-yield beet cultivation, and has promising agricultural application prospects.

The invention discloses a phosphate-solubilizing complex microbial inoculant suitable for medium and low-yield field soil and application thereof, and belongs to the technical field of microbial inoculants. The phosphate-solubilizing composite bacterial agent is prepared by mixing the following components according to a ratio of 1: 1: 1: 1: 1: (Stennotrophomonas sp. Strain nsu-56), Klebsiella pseudomonas sp. Strain KPNORMBLD13757, Sphingomonas sp. Strain B12 and Bacillus amyloliquefaciens strain LZ12-01, wherein the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, so that the phosphate-solubilizing composite bacterial agent can be used for preparing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent. The effective viable count of the prepared phosphate-solubilizing complex microbial inoculant is 1.4 * 10 < 9 >-1.6 * 10 < 9 > cfu / ml. The invention also provides a preparation method of the microbial inoculum, the microbial inoculum is subjected to expanding culture for 12-18 hours through a shaking table at 25-35 DEG C and 160-200r / min during preparation, and an embedding carrier material can be further adopted for embedding treatment to improve the stress resistance. The microbial agent is sprayed on the surface of soil, the dosage is 1 L / mu, soil phosphorus can be efficiently activated, crop growth is promoted, technical support is provided for soil improvement of medium and low-yield fields and crop yield increase, and the application prospect is wide.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention relates to the technical field of soil improvement effect evaluation, and discloses a rapid evaluation system and method for a microbial soil improvement effect, and the method comprises the following steps: preparing a test soil system containing a saline-alkali soil indigenous microbial community; setting a test group into which the microbial soil conditioner is introduced and a control group into which the conditioner is not introduced; applying a preset saline-alkali stress condition to trigger the reconstruction of the microbial function network; monitoring and comparing microbial function network metabolic flux of the test group and the control group during stress, wherein the microbial function network metabolic flux comprises a soil nitrogen conversion function network and a soil phosphorus activation function network; on the basis of the improvement degree of the metabolic flux of the test group relative to the control group, the ability of the modifier to reconstruct the microbial function network is characterized, and the ability is positively correlated with the field long-term improvement effect. The system and the method realize short evaluation period of the microbial soil improvement effect and accurate and reliable result, and are suitable for efficient screening, application and popularization of the microbial soil conditioner.

The invention discloses a preparation method and application of a calcium-phosphorus-loaded hydrothermal humic carbon-based soda saline-alkali soil slow-release conditioner, and belongs to the technical field of soda saline-alkali soil remediation and fertilizer. The invention aims to solve the problems that a modifier used in an existing soda saline-alkali soil improvement technology is short in validity period and inconvenient to transport and apply, possibly causes secondary environmental risks and cannot synergistically and continuously provide nutrients such as phosphorus for soil. The method comprises the following steps: 1, preparing phosphorus-rich hydrothermal humic carbon; 2, preparing sepiolite modified phosphorus-rich hydrothermal humic carbon; and 3, preparing a cross-linked protective layer. The invention breaks through the limitation of single function of the traditional saline-alkali soil remediation technology, develops the soda saline-alkali soil conditioner which is green, low in cost, easy to apply and high in stability and integrates multiple environment responses of high Na < + >, high pH and phosphorus starvation, and solves the problem of phosphorus starvation of the remediated soil while reducing the harm of soil salinization. And a green and sustainable solution is provided for treatment of soda saline-alkali soil.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The invention discloses a fertilizer applicable to wheat and capable of increasing the phosphorus utilization rate and a preparation method of the fertilizer, and aims to solve the problem of low phosphorus utilization rate of wheat. A three-layer composite structure of an inner layer quick-acting phosphorus core (monoammonium phosphate and humic acid chelated zinc and iron), a middle layer microbial slow-release layer (calcium alginate wrapped ammonium polyphosphate and glomus mosseae spores) and an outer layer intelligent response layer (chitosan-carboxymethyl cellulose immobilized phytase) is adopted, and processes of low-temperature drying, ellipse forming and the like are combined for preparation. The fertilizer realizes quick-acting-slow-release-intelligent conversion synergy, the phosphorus utilization rate is increased by 20-25% compared with the traditional phosphorus utilization rate, the microorganism survival rate is greater than or equal to 90%, the enzyme stability is improved, the soil phosphorus loss is reduced, and the application is convenient and efficient.

The invention relates to the technical field of environmental science, and discloses a soil phosphorus adsorption capacity evaluation method based on machine learning, which comprises the following steps: S1, firstly obtaining adsorption model parameter estimation results of multiple groups of phosphorus in different types of soil media and main physicochemical property data of soil, S2, preprocessing the obtained data, S3, establishing multiple machine learning models, and S4, evaluating the phosphorus adsorption capacity of the soil according to the machine learning models. Based on main physicochemical properties of the soil, predicting main parameters of the soil phosphorus adsorption model, S4, selecting an optimal machine learning model; s5, the optimal machine learning model is used for evaluating the phosphorus adsorption capacity of the regional soil, a large amount of data can be rapidly processed by adopting the machine learning model, a prediction result is given, the evaluation efficiency is improved, a time-consuming phosphorus adsorption experiment does not need to be conducted on each new soil sample, and therefore the sample testing cost is reduced, and the method is suitable for popularization and application. The adsorption parameters can be quickly predicted through the trained model only by measuring key physicochemical properties which are relatively easy to obtain, so that the detection speed is greatly improved.

The application provides application of a molybdenum-regulated synergistic substance phlorizin in promoting crop growth and activating soil phosphorus, and systematically studies the influence of exogenous application of phlorizin on soybean growth and on the form and content of phosphorus in soil. Through comparison of the difference between the experimental group and the control group, the role of phlorizin in promoting soybean growth and promoting the transformation of soil phosphorus is revealed, and meanwhile, the application of phlorizin significantly promotes the growth of soybean, which is specifically embodied in the increase of soybean plant height, the increase of the dry matter weight of the aboveground part and the root part and the like. Therefore, the application not only provides a brand-new growth promotion strategy for soybean production, but also opens an effective way for improving the utilization rate of soil phosphorus and improving the overall quality of soil.

The invention discloses a construction method of a novel composite biocontrol flora for preventing and treating cotton verticillium wilt, and relates to the technical field of biological prevention and treatment of agricultural plant diseases. The bacillus polymyxa and the bacillus cereus with the optimal antagonistic activity are respectively screened out through plate confrontation and buckling tests, and the bacillus pseudomycoides is obtained through screening by taking the growth promoting capability of dissolving phosphorus and producing indoleacetic acid as indexes. Through pairwise cross culture verification, the three strains are completely compatible and have no mutual inhibition effect. The method comprises the following steps: respectively and independently activating the three strains, carrying out enlarged culture, preparing single bacterial liquid with the OD600 value of 1.0, and uniformly mixing according to a volume ratio, so as to obtain the novel composite biocontrol flora for preventing and treating the cotton verticillium wilt. According to the invention, antagonistic bacillus and growth-promoting bacillus with complementary functions are screened, single bacteria are independently cultured and then are compounded according to a ratio of 1: 1: 1 to construct a composite flora, so that the synergistic interaction of efficient prevention and control of cotton verticillium wilt, cotton growth promotion and soil phosphorus solubilization is realized.

The invention relates to the technical field of microorganisms, in particular to pantoea BG4-1 with a growth promoting effect and application of the pantoea BG4-1. The pantoea BG4-1 is preserved in the China General Microbiological Culture Collection Center on June 20, 2025, and the preservation number of the pantoea BG4-1 is CGMCC NO.34960. The pantoea BG4-1 has relatively strong capabilities of dissolving phosphorus, dissolving potassium and producing IAA (Indoleacetic Acid), and has a remarkable growth promoting effect on capsicum and arabidopsis thaliana. According to the invention, a high-quality strain resource is provided for developing a novel efficient biological phosphorus-potassium fertilizer, the method has important meanings for improving the utilization rate of phosphorus and potassium elements in soil, reducing the application of chemical phosphorus-potassium fertilizers and promoting the sustainable development of agriculture, meanwhile, a new way is provided for resource utilization of invasive plants, and the ecological management concept of'taking harm as benefit 'is favorably realized.

The application belongs to the field of microorganisms, and relates to a phosphorus solubilizing fungus with sandy soil adaptability, in particular to a high-efficiency sandy soil phosphorus solubilizing Talaromyces HZ29 and a screening method and application thereof. Talaromyces pseudofuniculosus The preservation number of the Talaromyces HZ29 is CGMCC NO.42246, and the Talaromyces HZ29 is preserved in the China General Microbiological Culture Collection Center on October 15, 2025. The application first screens a new phosphorus solubilizing fungus strain, can efficiently activate the phosphorus in soil which is difficult to be directly absorbed by crops, improves the content of effective phosphorus in soil, has an outstanding phosphorus solubilizing effect, and has the abilities of iron carrier secretion and antagonism to plant disease bacteria and the function of promoting crop growth.

The invention relates to the technical field of microorganisms, and discloses pantoea H1 and a demonstration method and application thereof in agriculture assistance, the pantoea H1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36875. The invention further provides a microbial agent containing the bacterial strain, a preparation method and application of the bacterial strain in crop planting, the bacterial strain has efficient dual phosphorus solubilizing capacity, indissolvable inorganic phosphorus can be dissolved, organophosphorus can be mineralized, and the effectiveness of soil phosphorus is improved; meanwhile, the microbial agent has excellent saline-alkaline tolerance, can still keep high activity under adversity, and by applying the microbial agent in the seedling stage of crops, the rhizosphere microenvironment can be remarkably improved, root development and plant growth of the crops such as corn and mung beans can be promoted, the emergence rate and yield of the crops in soil such as saline-alkaline land can be increased, and the crop yield can be increased. And an excellent microbial germplasm resource is provided for activating soil nutrients, reducing weight and improving efficiency.

The application discloses a biodegradable coated urea added with zirconium-based MOF nanocrystals with phosphatase activity and a preparation method and application thereof, and belongs to the technical field of fertilizers. The zirconium-based MOF nanocrystals with phosphatase activity are prepared, a solution of the zirconium-based MOF nanocrystals is mixed with urea and dried, polypropylene carbonate is used as a coating material, and then the biodegradable coated urea added with the zirconium-based MOF nanocrystals with phosphatase activity is prepared through a fluidized bed solvent method. The coated urea prepared in the application can realize nitrogen control release, hydrolyze soil organic phosphorus through the phosphatase activity of the Zr-MOF nanocrystals, realize the dual functions of continuous supply of nitrogen and in-situ activation of soil phosphorus, simultaneously meet the demand of crops for two core nutrient elements of nitrogen and phosphorus, significantly relieve the low-phosphorus stress of crops, promote the growth of the root system and the aboveground part of crops, and improve the biomass of crops.

The invention discloses a detection method of inositol hexaphosphate and application thereof in plant cultivation, the electrostatic and chelation effects of nano iron oxide and inositol hexaphosphate are utilized to induce aggregation of nano particles to cause color change or UV-Vis absorption peak shift (200-220 nm), the detection method is used for rapidly detecting the concentration of inositol hexaphosphate in soil or a nutrient solution, the detection limit is 0.1 mg / L, the detection time is short, and the detection result is accurate. Total time lt; the time is 10 minutes. The method comprises the steps of sample extraction, reaction and optical determination, and is suitable for field monitoring of phosphorus fixation and fertilizer quality control. The detection result can guide the application of the compound, the phosphorus utilization efficiency is improved by 20-30%, and the plant cultivation effect is enhanced (for example, the alfalfa yield is increased by 18%, and the growth period of rice is shortened by about 7 days). The method provided by the invention is suitable for soil phosphorus fixation monitoring.

This application discloses a method and system for applying microbial fertilizer to promote phosphorus absorption in alfalfa. The method includes: determining the amount of microbial fertilizer to be applied to each alfalfa area based on the plant phosphorus content, total soil phosphorus content, and available soil phosphorus content; activating sensor equipment pre-installed in the alfalfa field to acquire alfalfa growth environment information and calculating the daily irrigation amount for the alfalfa field using the growth environment information; preparing a fertilizer solution for a drip irrigation fertilization system based on the microbial fertilizer application amount and the daily irrigation amount, and using the fertilizer solution to perform irrigation fertilization on the alfalfa field to complete the application of microbial fertilizer to promote phosphorus absorption in alfalfa. Through the solution of this application, the dissolution and release of phosphorus in the soil can be promoted, thereby improving the phosphorus absorption efficiency of alfalfa.