97 results about "Soil phosphorus" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention discloses a method for immobilizing growth-promoting rhizobacteria by taking decarburized gangue-based biochar as a carrier, a solid waste-based microorganism immobilization ball prepared by the method and application of the solid waste-based microorganism immobilization ball, and relates to the technical field of solid waste utilization. The method comprises the following steps: preparing gangue-based zeolite by microwave activation and decarburization of coal gangue; preparing gangue-based biochar by utilizing phosphorus modification; and preparing the solid-waste-based microorganism solidification balls by utilizing an adsorption-embedding-crosslinking method. The decarburized gangue-based biochar is used as a carrier material for immobilizing the growth-promoting rhizosphere bacteria, and is used for restoring the sandy degraded soil in the northwest arid mining area. According to the technology, the survival time and functional activity of the growth-promoting rhizobacteria in the soil environment can be improved, and a new way is provided for consumption of bulk gangue solid waste. The method also has the technical advantages of improving the carbon source utilization rate of the gangue and increasing the phosphate fertilizer and the organic carbon library of the soil.

The invention discloses a preparation method of iron-modified biochar, which comprises the following steps: soaking biochar in an acid solution, taking out the biochar, recovering the biochar to be neutral, drying, mixing the dried biochar with an iron salt solution, fully reacting, drying and calcining to obtain the iron-modified biochar; the mass ratio of the dried biochar to iron in the iron salt solution is 0.28-0.84. The iron modified biochar has excellent phosphorus adsorption capacity in practical application, and has excellent phosphorus solid storage and continuous release capacity; the iron modified biochar can be used for soil phosphorus fixation and slow release, water pollution prevention and / or phosphate fertilizer saving, and can effectively promote plant growth.

The invention relates to bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.32593 as well as preparation and application of a microbial agent of the bacillus velezensis, and belongs to the technical field of microorganisms. The strain disclosed by the invention has the effects of dissolving phosphorus, dissolving potassium, fixing nitrogen and producing indoleacetic acid (IAA) at the same time. The method comprises the following steps: inoculating bacillus velezensis CGMCC No.32593 into an LB liquid culture medium, and fermenting by using a 5L fermentation tank to obtain the liquid microbial agent. The microbial agent provided by the invention is simple in preparation method and convenient for industrial production. By applying the liquid microbial agent, the plant height of tomatoes and the fresh weight of overground parts can be effectively increased, and the utilization rate of soil phosphorus in crop production is increased.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention discloses application of jointly inoculated rhizosphere phosphate solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms. The acinetobacter and pantoea combined inoculation provided by the invention can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, thereby promoting the utilization of phosphorus. The ratio of acinetobacter to pantoea combined inoculation is (4-6): (6-4), the soil phosphorus dissolving capacity and the plant growth promoting effect are remarkable, and the compound fertilizer can be applied to improving the soil phosphorus utilization rate and promoting plant growth and has important agricultural and environmental values.

The invention discloses a phosphorus-efficient choerospondias axillaris family breeding method, and relates to the technical field of agricultural breeding, and the method comprises the following steps: step S4, index calculation, and according to the above data, selecting the choerospondias axillaris family; calculating the root-crown ratio, the total phosphorus utilization efficiency, the low-phosphorus stress resistance coefficient of each index, the membership function value of the comprehensive index, the weight of each comprehensive index, the comprehensive value of the low-phosphorus resistance efficiency, and the comprehensive value of the phosphorus efficiency under different phosphorus supply conditions; and S5, screening low-phosphorus-resistant identification key indexes, performing stepwise regression analysis by taking the low-phosphorus-resistant efficiency comprehensive evaluation value as a dependent variable and the low-phosphorus-resistant stress coefficient of the measured index as an independent variable to obtain a multiple regression equation, and screening out the low-phosphorus-resistant identification index of the choerospondias axillaris. The problems of long time consumption, large workload, no breeding system and the like of the traditional breeding method are solved. According to the method, the choerospondias axillaris families which are resistant to low phosphorus and efficient in phosphorus and aim at the problem that phosphorus in soil is generally lacked are screened out preferentially, existing germplasm resources are sequenced according to screening targets, optimal germplasm resources are screened out, and the target range is narrowed.

The invention relates to the technical field of agricultural production, and discloses a salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 and application thereof in corn planting, the salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 is a purple blue bacterium, the preservation number of the strain is CGMCC No.42313, and the strain has multiple functions of resisting salt and alkali, dissolving insoluble phosphorus and degrading 2-methyl-4-chlorophenoxyacetic acid pesticide residues. The strain, a culture thereof or a microbial inoculum prepared from the strain are applied to agricultural production, and plant growth is promoted by exerting the composite biological function of the strain under saline-alkali stress. According to the invention, the synergistic effect of saline-alkaline tolerance, phosphate solubilization and pesticide degradation of the strain is utilized, so that the plant growth inhibition is relieved in the adverse situation, the soil phosphorus bioavailability is improved, the pesticide-polluted soil is repaired, and the plant seed germination, root development and plant growth are promoted. The strain has an important application prospect in the aspect of developing a microbial agent for saline-alkali soil improvement.

The invention relates to a composite phosphate solubilizing bacterium agent based on biochar carriers and a preparation method and application thereof.The composite phosphate solubilizing bacterium agent can effectively improve the phosphorus absorption and utilization rate of plants in the growth process, and by immobilizing inorganic phosphorus dissolving bacteria on different types of biochar carriers, the yield of the plants is improved more remarkably. The method has the advantages that the method can be used for improving the efficiency of phosphorus in barren soil, reducing dependence on chemical phosphate fertilizer and improving the efficiency of soil phosphorus and phosphorus absorption of crops, especially phosphorus absorption capacity and growth performance of oilseed rape, has a wide agricultural application prospect, and is a method for realizing more environment-friendly and sustainable agricultural production, and the method is particularly used for improving the efficiency of phosphorus in barren soil, reducing dependence on chemical phosphate fertilizer and improving the efficiency of soil phosphorus and phosphorus absorption of crops.

The invention discloses a bacillus preparation, a bacillus compound preparation and application of the bacillus preparation and the bacillus compound preparation in increasing ferrophosphorus in soil, and belongs to the field of plant nutrition and microbiology. The preparation method comprises the following steps: centrifuging a bacterial solution of bacillus 1603IPR-02, and collecting supernate to obtain the siderophore preparation. The siderophore preparation is applied to crop root systems, southern soil or compounded with phosphate tailings to form a bacillus compound preparation to be applied to crops, plant growth can be promoted, plant biomass can be increased, meanwhile, the siderophore preparation has the efficient Fe-P activation capacity, Fe-P insoluble in soil is converted into phosphorus and iron capable of being utilized by plants, and the utilization rate of the Fe-P is increased. The bio-availability of soil nutrients is improved, and the absorption of crops on phosphorus and iron in soil is promoted. When the bacillus preparation or the bacillus compound preparation is used for treating pakchoi, the dry weight, the phosphorus content and the iron content of the pakchoi as well as the quick-acting phosphorus content and the effective iron content of soil can be increased. When applied to crops, the preparation has the characteristics of no pollution, no residue and biological environmental protection.

The invention relates to sulfate reducing bacteria with phosphate solubilizing capacity and application of the sulfate reducing bacteria in soil ecological restoration, and belongs to the technical field of soil environment microorganisms. The sulfate reducing bacterium disclosed by the invention is Desulfosporosinus sp.QY25, and is preserved in the Guangdong Microbial Culture Collection Center on August 6, 2024, and the preservation number of the sulfate reducing bacterium is GDMCC No: 64963. The invention provides a sulfate reducing bacterium Desulfosporosinus sp.QY25 which is high in dissimilatory sulfate reducing capacity and can be used for converting insoluble phosphorus in soil into soluble phosphorus which can be absorbed by plants. The invention further provides a preparation method of the sulfate reducing bacterium Desulfosporosinus sp.QY25 and an application of the sulfate reducing bacterium Desulfosporosinus sp.QY25. The strain can restore soil ecology, neutralize pH, reduce the content of SO4 < 2->, reduce the bioavailability of heavy metals, improve the utilization efficiency of phosphorus in soil, improve the activity of acid phosphatase in tailings and enable plants to grow well.

The invention belongs to the field of microorganisms, and relates to a phosphate-solubilizing fungus with sandy soil adaptability, in particular to a high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain as well as a screening method and application of the high-efficiency sandy soil phosphate-solubilizing talaromyces HZ29 strain. The talaromyces sp. HZ29 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.42246, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on October 15, 2025. A new phosphorus-solubilizing fungus strain is obtained through screening for the first time, phosphorus which is difficult to be directly absorbed by crops in soil can be efficiently activated, the content of available phosphorus in the soil is increased, and the phosphorus-solubilizing fungus strain has an outstanding phosphorus-solubilizing effect; and the strain has siderophore secretion capability and disease bacterium antagonism capability, and has a growth promoting function on crops.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a composite solid phosphorus-solubilizing bacterial agent for repairing cadmium-polluted soil and improving soil quality, wherein the composite solid phosphorus-solubilizing bacterial agent comprises phosphorus-solubilizing bacteria, bone charcoal, culture medium and organic matrix; the phosphorus-solubilizing bacteria are Pantoea agglomerans CT-3 and Enterobacter hormaechei HS-6; the composite solid phosphorus-solubilizing bacterial agent is applied to Cd-polluted soil, and the biological availability of Cd in the soil is reduced by Pantoea agglomerans CT-3, Enterobacter hormaechei HS-6 and micron-nanometer multi-stage bone charcoal, and meanwhile, the soil quality is improved. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of effective soil phosphorus is increased by 98.19%; on the other hand, the application can fix soil heavy metal Cd, so that the content of effective Cd in the soil is reduced by about 52.50%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in repairing farmland Cd pollution, improving soil quality and environmental protection.

The invention discloses a high-organic-matter biological matrix and a preparation method thereof. The biological matrix is prepared from the following components in percentage by mass: 20% of imported peat, 30% of coco coir, 30% of a fermented material consisting of tobacco powder and livestock and poultry manure, and 20% of perlite. The preparation method comprises the following steps: uniformly mixing tobacco powder, livestock and poultry manure, imported peat and coco coir, composting and fermenting for not less than 45 days, controlling the temperature not to exceed 72 DEG C, crushing, screening, storing and cooling, adding microbial strains, and carrying out anaerobic fermentation in a packaging bag for 7 days; the organic matter content of the obtained biological matrix exceeds 45%, and the biological matrix can effectively loosen soil, promote crop root growth and release phosphorus and potassium elements in soil, can be used for field crop and vegetable seedling raising and the like, and has the advantages of being safe, environmentally friendly and remarkable in soil improvement effect.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

The invention is applicable to the technical field of soil improvement, provides a method for improving soil by inputting organic materials and application, and aims to solve the degradation problems of soil hardening and nutrient imbalance of Yuluihu dry-hot valley vineyards in the method, cow dung, fungus bags and mixtures of the cow dung and the fungus bags are used as organic materials, and random block test design is adopted. The method comprises the following steps: dividing a degraded vineyard into plots and test plots with specific specifications, applying organic materials, and measuring the water retention capacity, aggregate composition and nutrient element content of soil through layered soil sampling to realize soil quality regulation and control. Adaptive organic materials can be selected according to soil problems and grape growth requirements, the cow dung can remarkably improve the phosphorus content of the soil, the cow dung and the fungus bags can optimize the soil aggregate structure, the water and fertilizer retention capacity, ventilation and water permeability of the soil are effectively improved, technical support is provided for sustainable development of the dry-hot valley grape industry, and the application prospect is wide.

The invention discloses a comprehensive nutrition regulation and control method for improving overwintering cold resistance of citrus fruit trees, and relates to the field of fruit tree cultivation, and the method comprises the following steps: S1, soil conditioning and mycorrhiza inoculation after fruit picking: determining the content of rapidly available phosphorus in orchard soil, when the content of rapidly available phosphorus is greater than 30mg / kg, firstly applying humic acid and calcium magnesium phosphate fertilizer for phosphorus reduction treatment, and after the content of rapidly available phosphorus is reduced to be less than or equal to 20mg / kg, then applying a nutrient solution; digging radial short ditches on the inner side of a water drip of a tree crown, mixing a pre-activated arbuscular mycorrhizal fungus inoculant with an organic carrier, filling the mixture into the ditches, and earthing and watering; according to the comprehensive nutrition regulation and control method for improving the overwintering cold resistance of the citrus fruit trees, through soil phosphorus pre-regulation and local enrichment inoculation methods, the mycorrhizal fungus colonization rate is increased to 50% or above from less than 20%, and the mycorrhizal inoculation effect and the input-output ratio are remarkably improved.

The invention discloses a method for preparing slow-release phosphate fertilizer soil from graphite tailing sand and coal gangue and application, and relates to the technical field of solid waste resource utilization and agricultural fertilizer. The slow-release phosphate fertilizer soil is prepared by carrying out gradient magnetic separation-acid leaching synergistic pretreatment on graphite tailing sand (the removal rate of magnetic impurities reaches 92%-95%), carrying out segmented roasting-microwave activation treatment on coal gangue (the specific surface area is increased to 28.6-32.4 m < 2 > / g), then mixing the pretreated raw materials with a composite exciting agent, and carrying out a low-temperature sintering-granulation molding process. The problem of low resource utilization rate of the graphite tailing sand and the coal gangue is effectively solved, the soil phosphorus slow release period of the prepared phosphate fertilizer reaches 120-150 days, the prepared phosphate fertilizer can meet the nutrient requirement of phosphorus-loving crops in the whole growth period, compared with a traditional phosphate fertilizer, the usage amount is reduced by 30-40%, the phosphorus utilization rate is increased to 65-72%, and remarkable economic, environmental and social benefits are achieved.

The invention discloses subterranean intergeneric bacillus 52-6 with a phosphate solubilizing function and application thereof, and belongs to the technical field of microorganisms. The invention provides subterranean intergeneric bacillus, which is classified and named as subterranean intergeneric bacillus, and the preservation number of the subterranean intergeneric bacillus is CGMCC (China General Microbiological Culture Collection Center) No.32719. The subterranean intergeneric bacillus is named as subterranean intergeneric bacillus. The bacterial colony of the subterranean intergeneric bacillus 52-6 is round, light coral red, semitransparent, flat in surface and tidy in edge, the suitable growth temperature is 35-38 DEG C, the delay phase is short, the growth is rapid, and the culture is easy; the subterranean intergeneric bacillus 52-6 can efficiently dissolve phosphate insoluble in soil and promote absorption of plants to soil phosphorus elements, has the effect of promoting plant growth, effectively improves soil fertility and reduces use of chemical fertilizers, and has huge potential in application of promoting plant growth and improving soil fertility.

The application discloses application of combined inoculation of rhizosphere phosphorus solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms.The combined inoculation of acinetobacter and pantoea provided by the application can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, and promotes the utilization of phosphorus.The combined inoculation of acinetobacter and pantoea has significant soil phosphorus solubilizing capacity and plant growth promotion effect when the ratio of acinetobacter to pantoea is (4-6):(6-4), and can be applied to improving soil phosphorus utilization rate and promoting plant growth, and has important agricultural and environmental values.

This invention belongs to the field of agricultural biotechnology, specifically relating to the application of a compound biological agent in agriculture. The compound biological agent comprises live engineered Bacillus subtilis bacteria and a 3% glucose solution; wherein the engineered Bacillus subtilis bacteria are obtained through genetic modification using Bacillus subtilis as the substrate bacteria, and the live bacteria concentration is ≥1×10¹. 0 The 3% glucose solution (CFU / mL) is a 3% (w / v) aqueous solution of glucose, and the two are mixed at a volume ratio of 1:(800-1200). This invention's compound biological agent combines the soil microecological regulation and root growth promotion capabilities of Bacillus subtilis engineered live bacteria with the sugar metabolism regulation effects of 3% glucose. The synergistic effect of these two agents significantly promotes beet root growth, increases the activity of beet sugar metabolism enzymes, and increases the accumulation of sucrose and soluble sugars in the tubers. Simultaneously, it improves the rhizosphere soil microecological environment of beet and enhances soil phosphorus activation capacity. This provides an environmentally friendly and efficient biological agent for high-quality and high-yield beet cultivation, and has promising agricultural application prospects.

The invention discloses a phosphate-solubilizing complex microbial inoculant suitable for medium and low-yield field soil and application thereof, and belongs to the technical field of microbial inoculants. The phosphate-solubilizing composite bacterial agent is prepared by mixing the following components according to a ratio of 1: 1: 1: 1: 1: (Stennotrophomonas sp. Strain nsu-56), Klebsiella pseudomonas sp. Strain KPNORMBLD13757, Sphingomonas sp. Strain B12 and Bacillus amyloliquefaciens strain LZ12-01, wherein the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, and the concentration of each bacterial strain is gt, so that the phosphate-solubilizing composite bacterial agent can be used for preparing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent for producing the phosphate-solubilizing composite bacterial agent. The effective viable count of the prepared phosphate-solubilizing complex microbial inoculant is 1.4 * 10 < 9 >-1.6 * 10 < 9 > cfu / ml. The invention also provides a preparation method of the microbial inoculum, the microbial inoculum is subjected to expanding culture for 12-18 hours through a shaking table at 25-35 DEG C and 160-200r / min during preparation, and an embedding carrier material can be further adopted for embedding treatment to improve the stress resistance. The microbial agent is sprayed on the surface of soil, the dosage is 1 L / mu, soil phosphorus can be efficiently activated, crop growth is promoted, technical support is provided for soil improvement of medium and low-yield fields and crop yield increase, and the application prospect is wide.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention relates to the technical field of soil improvement effect evaluation, and discloses a rapid evaluation system and method for a microbial soil improvement effect, and the method comprises the following steps: preparing a test soil system containing a saline-alkali soil indigenous microbial community; setting a test group into which the microbial soil conditioner is introduced and a control group into which the conditioner is not introduced; applying a preset saline-alkali stress condition to trigger the reconstruction of the microbial function network; monitoring and comparing microbial function network metabolic flux of the test group and the control group during stress, wherein the microbial function network metabolic flux comprises a soil nitrogen conversion function network and a soil phosphorus activation function network; on the basis of the improvement degree of the metabolic flux of the test group relative to the control group, the ability of the modifier to reconstruct the microbial function network is characterized, and the ability is positively correlated with the field long-term improvement effect. The system and the method realize short evaluation period of the microbial soil improvement effect and accurate and reliable result, and are suitable for efficient screening, application and popularization of the microbial soil conditioner.

The invention discloses a preparation method and application of a calcium-phosphorus-loaded hydrothermal humic carbon-based soda saline-alkali soil slow-release conditioner, and belongs to the technical field of soda saline-alkali soil remediation and fertilizer. The invention aims to solve the problems that a modifier used in an existing soda saline-alkali soil improvement technology is short in validity period and inconvenient to transport and apply, possibly causes secondary environmental risks and cannot synergistically and continuously provide nutrients such as phosphorus for soil. The method comprises the following steps: 1, preparing phosphorus-rich hydrothermal humic carbon; 2, preparing sepiolite modified phosphorus-rich hydrothermal humic carbon; and 3, preparing a cross-linked protective layer. The invention breaks through the limitation of single function of the traditional saline-alkali soil remediation technology, develops the soda saline-alkali soil conditioner which is green, low in cost, easy to apply and high in stability and integrates multiple environment responses of high Na < + >, high pH and phosphorus starvation, and solves the problem of phosphorus starvation of the remediated soil while reducing the harm of soil salinization. And a green and sustainable solution is provided for treatment of soda saline-alkali soil.

The invention belongs to the technical field of microorganisms, and particularly relates to a phosphate solubilizing strain PSBS-2 and application thereof. The invention relates to a phosphorus-solubilizing strain PSBS-2, the taxonomic name of the phosphorus-solubilizing strain PSBS-2 is Niallia circulans, the phosphorus-solubilizing strain PSBS-2 is preserved in Guangdong Microbial Culture Collection Center on May 14, 2025, and the preservation number is GDMCC No: 66329. According to the invention, a phosphorus-solubilizing bacterial strain is screened to obtain a bacterial strain PSBS-2, and the bacterial strain PSBS-2 is determined to be Niallia circulans through morphological observation and molecular biological identification. Phosphorus-solubilizing ability tests on the strain PSBS-2 find that the strain PSBS-2 has relatively strong phosphate-solubilizing ability and can convert insoluble phosphate into an absorbable and usable form, which is of great significance in improving the utilization rate of soil phosphorus, maintaining soil health and realizing sustainable development of agricultural economy. Meanwhile, strain resource support can be provided for developing related microbial agents, biological fertilizers or soil conditioners.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The invention discloses an application of Pantoea cypripedii in soil phosphate solubilization and plant growth promotion, and belongs to the technical field of microorganisms. The Pantoea cypripedii provided by the invention can be used for effectively converting insoluble inorganic phosphorus into soluble inorganic phosphorus, so that the utilization of phosphorus is promoted. The strain can also adapt to the soil environment, can improve the utilization efficiency of phosphorus in soil and promote the growth of plants when being added into the soil, can also improve the nutrient condition of the soil, and has important agricultural and environmental values.