11 results about "Aesculin" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The application discloses a chitosan composite film and a preparation method and application thereof, and belongs to the technical field of food preservation materials. The chitosan composite film comprises the following raw materials: chitosan, zein, Span 80, a natural eutectic solvent and aesculin. The preparation method comprises the following steps: mixing chitosan solution, zein solution, Span 80, the natural eutectic solvent and aesculin, stirring and ultrasonic treatment, and preparing a film-forming solution; and the film-forming solution is dried through a flow casting method to prepare the chitosan composite film. That is, the chitosan is blended with the zein, the natural eutectic solvent is used for plasticizing, and the aesculin is used for functionalization, so that the mechanical properties, the antibacterial property and the antioxidant property of the film are synergistically improved, and the fish preservation period is significantly prolonged.

The present application relates to a preparation method and application of Cortex Fraxini extract with pine wood nematode killing activity, wherein dry powder of Cortex Fraxini is extracted by ethanol to obtain an extract solution, and the ethanol extract obtained after reduced pressure distillation is extracted by ethyl acetate-water liquid-liquid extraction, the ethyl acetate phase is reduced pressure distilled to obtain the ethyl acetate extract of Cortex Fraxini with strong pine wood nematode killing activity, and the main active ingredient of the extract, aesculin, is separated therefrom. The corrected mortality rate of the ethyl acetate extract of Cortex Fraxini to pine wood nematode is as high as 94.14% at a concentration of 1 mg / mL within 72 h; and the half lethal concentration of aesculin to pine wood nematode is 30.37 μg / mL. The present application develops a new use of Cortex Fraxini extract, and provides an effective plant-derived pine wood nematode killing active substance, the raw material source is convenient, the extraction method is simple, the cost is low, the Cortex Fraxini extract has development potential in controlling pine wood nematode disease, and is applied to the development of an environmentally friendly plant-derived pine wood nematode killing agent.

The application provides a separation and purification method of aesculin, aesculetin and aesculin in cortex fraxini, which comprises the following steps: firstly, heating extraction is performed on the crushed cortex fraxini raw material; secondly, plate and frame pressure filtration is performed on the obtained extract, and the filtrate is subjected to chromatographic separation on a macroporous resin column; then, 10-30% ethanol is used for resolving the macroporous resin column and purifying aesculin; subsequently, 50-80% ethanol is used for resolving the macroporous resin column, and the resolving liquid is subjected to enzymolysis and aesculetin purification; finally, the enzymolysis mother liquor is treated and aesculin is purified. The multi-layer separation and purification scheme can simultaneously and efficiently separate and purify the three high-value active components of aesculin, aesculetin and aesculin in cortex fraxini, greatly improves the comprehensive utilization rate of cortex fraxini resources, creates higher product value, reduces the use amount of organic solvents, reduces the production cost, reduces the pollution to the environment, and meets the green and environmental protection production concept.

The application provides a feruloyl-CoA 6'-hydroxylase pocket mutant and application. The feruloyl-CoA 6'-hydroxylase pocket mutant is obtained by amino acid mutation of the amino acid sequence shown in SEQ ID NO. 1, and includes at least one of the following amino acid mutations: L141N, L141G, A142N, Q144D, F156Y, V157T, N311G. The application also provides an engineering bacterium for synthesizing coumarin compounds by using the feruloyl-CoA 6'-hydroxylase mutant. The feruloyl-CoA 6'-hydroxylase mutant has good enzyme activity and stability, so that the engineering bacterium can not only realize efficient synthesis of coumarin compounds such as scopoletin, aesculin, aesculin glycoside and anisodamine, but also realize efficient de novo biosynthesis of the coumarin compounds, thereby providing a reference for microbial synthesis of the coumarin compounds.

The invention discloses a chitosan composite film and a preparation method and application thereof, and belongs to the technical field of food fresh-keeping materials, the chitosan composite film comprises the following raw materials: chitosan, zein, Span 80, a natural eutectic solvent and aesculin; the preparation method comprises the following steps: mixing a chitosan solution, a zein solution, span 80, a natural eutectic solvent and aesculin, and carrying out stirring and ultrasonic treatment to prepare a film-forming solution; and drying the film forming solution through a tape casting method to obtain the chitosan composite film. According to the invention, chitosan and zein are blended, and the natural eutectic solvent is used for plasticizing and aesculin is used for functionalization, so that the mechanical property, antibacterial property and oxidation resistance of the film are synergistically improved, and the fresh-keeping period of fish meat is remarkably prolonged.

The invention discloses application of akebia quinata phenylethanoid glycoside B in preparation of a product for preventing and treating influenza virus infection, and relates to the technical field of biological medicines. Through molecular docking screening, cytotoxicity experiments and antiviral activity verification, the application value of the akebia quinata phenylethanoid glycoside B in prevention and treatment of influenza virus infection is defined, and the technical effect is remarkable. In the aspect of safety, the akebia quinata phenylethanoid glycoside B has no obvious influence on the survival rate of MDCK cells under high concentration, is far higher than clinical potential medication concentration, has no obvious cytotoxicity and is good in biocompatibility. In the aspect of antiviral activity, the akebia quinata phenylethanoid glycoside B shows an efficient inhibition effect on influenza viruses, the IC50 value is as low as 28.5 mu M, and the inhibition effect is superior to that of part of natural components and oseltamivir. In an application scene, the akebia quinata phenylethanoid glycoside B can be prepared into various drug dosage forms and can also be used as an active ingredient of a disinfectant, and a novel safe, efficient and multifunctional influenza prevention and control scheme is provided for the field of biological medicine.

The present application relates to the field of medicine, and more particularly to the use of aesculin or verbascoside in the preparation of a medicament for treating and / or preventing IgAN. The present inventors have found that aesculin and / or verbascoside can significantly improve proteinuria caused by IgAN and reduce inflammatory reactions, thereby playing a role in protecting kidney function, and do not have the serious shortcomings of clinically commonly used drugs, indicating that aesculin and / or verbascoside have a strong and low-toxicity effect in treating IgAN. In addition, aesculin and / or verbascoside are a natural compound, low toxicity and easy to be accepted by the human body.

The application belongs to the technical field of biological medicine, and particularly relates to application of aesculin B in preparation of a medicine for preventing and / or treating gouty nephropathy. The aesculin B can reduce a kidney index, reduce contents of serum creatinine, urea nitrogen and uric acid, reduce kidney crystal precipitates and resist renal interstitial fibrosis, so that an effect of preventing and / or treating gouty nephropathy is achieved.

The invention discloses application of aesculin in improving the colonization rate of arbuscular mycorrhizal fungi in plants, and relates to the technical field of biology. The research finds that aesculin has a positive promoting effect on colonization of arbuscular mycorrhizal fungi in plants. Through exogenous application of aesculin to the fraxinus mandshurica seedlings, the colonization rate of the mycorrhizal fungi root interior root cyst is improved by 10.53%. The verification of the Arabidopis thaliana aesculin synthesized mutant (f6'h) shows that the infection rates of the wild type and the mutant are respectively increased by 6.49% and 25.80% compared with the control group under the conditions that the arbuscular mycorrhizal fungi are inoculated and aesculin is applied. The invention provides important technical support for promoting forest growth and optimizing a forest cultivation scheme by means of arbuscular mycorrhizal fungi and related secondary metabolites.