Flat-plate sieving method of aesculin for high-yield beta-glucosidase producing strain

A technology of glucosidase and escin, applied in biochemical equipment and methods, microbial determination/inspection, enzymes, etc., can solve the problems of expensive reagents, cumbersome methods, etc., and achieve reliable results, simple operation, and obvious price. The effect of advantage
CN101177699AInactive Publication Date: 2008-05-14SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2008-05-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a flat plate screening method for high yielding Beta-glucosidase production bacteria by using aesculin. The method comprises the steps of: 1. earth sample collection; 2. enrichment culture; 3. flat plate preparation of the aesculin; 4. flat plate primary screening aesculin; 5. separation and purification; 6. secondary screen by a shaking battle, etc. The invention can fast screen active strain possessing the Beta-glucosidase from samples which contain rich fibrin resources; compared with the method which is reported in published literature which screens Beta-glucosidase production bacteria by using a cellobiose flat plate method, the invention has the remarkable advantages at price; under the condition that obtains 100 strains by screening, the cost of the invention by using the aesculin is only one tenth of the cellobiose flat plate method; in addition, the invention has the advantages of simple operation, reliable result, etc.
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Description

technical field

[0001] The invention relates to plate screening of β-glucosidase-producing bacteria, in particular to a method for using aescin for plate screening of high-yielding β-glucosidase-producing bacteria. Background technique

[0002] In the process of hydrolyzing natural cellulose into glucose, it must rely on the synergistic action of three enzymes, endo-cellulase, exo-cellulase and β-glucosidase. Among them, β-glucosidase is the rate-limiting enzyme in this process, and the level of its enzyme activity directly affects the level of the overall enzyme activity of the cellulase system. Most of the most widely used cellulase producing strains are excellent mutant strains of Trichoderma reesei. Although these strains can produce endo- and exo-cellulase with high activity, due to the low activity of β-glucosidase, in the process of hydrolyzing cellulose with cellulase, the accumulation of cellobiose, cellobiose Sugar, in turn, forms a strong feedback inhibition of ...

Claims

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