453 results about "Alglucerase" patented technology

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses an enzyme immobilization method based on charge directed crosslinking, and belongs to the technical field of enzyme immobilization. The method sequentially comprises the following steps: reacting an enzyme with a modifier containing an anionic group to generate an electronegative modified enzyme; dissolving a protonated amino carrier in an acidic buffer solution to form a first solution, and dispersing a dissociation anion group-containing carrier and a modification enzyme in an alkaline buffer solution to form a second solution; mixing the two solutions, and constructing a gel network immobilized enzyme through electrostatic crosslinking; and finally, strengthening the gel network by using a multivalent metal ion solution. Directional anchoring of the enzyme in the gel is achieved through charge matching, the network stability and the enzyme microenvironment are synchronously optimized in combination with metal ion coordination, use of a covalent cross-linking agent is avoided in the whole process, conformation damage of an enzyme active center is avoided, the activity retention rate, the operation half-life period and the substrate mass transfer efficiency of the immobilized enzyme are remarkably improved, and the immobilized enzyme has good application prospects. The method is suitable for efficient immobilization of industrial enzymes such as beta-glucosidase, and has high biocompatibility and industrial potential.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention discloses a method for improving the quality of a Tieguanyin tea extracting solution through segmented composite enzymolysis extraction, and belongs to the technical field of tea drink preparation. The method comprises the following steps: S1, performing low-temperature aroma extraction on Tieguanyin dry tea, and performing crushing so as to obtain crushed tea; s2, adding water and beta-glucosidase into the broken tea to carry out first-stage enzymolysis extraction, and separating to obtain tea aroma extract liquid and aroma-free tea residues; s3, water and composite hydrolase are added into the aroma-free tea residues for second-stage enzymolysis extraction, separation and filtration are performed, and a hydrolysis extraction solution is obtained; and S4, mixing the tea fragrance extract liquid with the hydrolysis extract liquid, cooling, centrifuging and filtering to obtain the Tieguanyin tea extract liquid. According to the method disclosed by the invention, the original flavor of the Tieguanyin is maintained, meanwhile, the fragrance and the mouth feel are remarkably improved, and the problems of fragrance loss and bitter taste caused by traditional high-temperature long-time extraction and cooked soup taste generated by tannase enzymolysis are avoided.

The invention discloses an agaric polypeptide VR5 with alpha-glucosidase inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the auricularia auricula polypeptide VR5 is VGMLR, and the auricularia auricula polypeptide VR5 can be prepared by a solid-phase synthesis method and an enzymolysis method (flavourzyme). The agaric polypeptide VR5 has the beneficial effects that experiments prove that the agaric polypeptide VR5 has very high alpha-glucosidase inhibitory activity, IC50 is equal to 34.96 mu g / mL, the agaric polypeptide VR5 can be used as an alpha-glucosidase inhibitor for preventing or treating alpha-glucosidase mediated diseases, and the agaric polypeptide VR5 can be used for preventing or treating alpha-glucosidase mediated diseases. Or as a hypoglycemic active component, the composition is added into auxiliary hypoglycemic functional food for auxiliary regulation of the blood sugar level of hyperglycemia people.

The invention belongs to the technical field of microorganisms and biological medicines, and particularly relates to marine-source lactobacillus plantarum HYC-1 with cholesterol lowering and alpha-glucosidase inhibiting activity and application of the marine-source lactobacillus plantarum HYC-1. The novel lactobacillus plantarum HYC-1 is screened from intestinal tracts and contents of marine fishes, the strain has efficient cholesterol lowering activity and alpha-glucosidase inhibition activity at the same time, and experimental results show that the in-vitro cholesterol lowering rate of lactobacillus plantarum HYC-1 fermentation liquor can reach 89.4%, the alpha-glucosidase inhibition rate can reach 93.5%, and the lactobacillus plantarum HYC-1 fermentation liquor can be used for preparing the cholesterol lowering agent. One bacterium has double effects and unique functions; besides, the strain shows excellent tolerance and high safety, so that the strain can be widely applied to food, health care products and medicines, especially to preparation and research and development of medicines with the efficacy of regulating blood fat and blood sugar.

The invention relates to a multi-component ginseng extract containing ginseng polypeptide and a preparation method of the multi-component ginseng extract. The preparation method comprises the following steps: S1, preparing ginseng powder from a ginseng raw material; mixing the ginseng powder with an acidic buffer solution, and carrying out presoaking treatment and homogenate dissolution promoting treatment to obtain ginseng primary pulp; s2, mixing the wall-breaking enzyme system with the ginseng primary pulp, and performing acid wall-breaking treatment to obtain wall-broken primary pulp; mixing an invertase system with the wall-broken primary pulp, and carrying out enzymolysis synchronous glucoside conversion treatment to obtain a crude extract; s3, filtering and separating the crude extracting solution to obtain an extract containing the ginseng polypeptide; the pH values of the ginseng primary pulp, the wall-broken primary pulp and the crude extract are in a range of 4.0-6.0; the wall breaking enzyme system comprises pectinase and cellulase; the invertase system comprises beta-glucosidase and acid protease. The technical problems that in the prior art, a ginseng extract is complex in extraction process, multiple active ingredients are difficult to efficiently extract at the same time, the product quality is low, and the biological activity is poor are solved.

The technical scheme of the invention discloses black fungus protein capable of reducing blood glucose and resisting oxidation as well as a preparation method and application of the black fungus protein. The amino acid sequence of the black fungus protein is as shown in SEQ ID NO. 1. The protein which is separated and identified from the black fungus for the first time and has the effects of reducing blood glucose and resisting oxidation achieves the effect of reducing blood glucose through double inhibition of alpha-amylase and alpha-glucosidase, meanwhile, the protein has remarkable antioxidant activity, animal experiments prove that fasting blood glucose and body weight of a type II diabetes model mouse can be effectively reduced, and the protein has a good application prospect. The compound has high biological activity and safety, and can be used for developing functional products with hypoglycemic and antioxidant activity.

Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa; at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof; and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to a beta-glucosidase mutant with high catalytic activity and application of the beta-glucosidase mutant. The sequence of the beta-glucosidase mutant is obtained by carrying out any one of the following mutations on an amino acid sequence as shown in SEQ ID No.1: respectively mutating proline at the 95th site and arginine at the 178th site into cysteine; phenylalanine at the 98th site and arginine at the 178th site are respectively mutated into cysteine. The obtained mutants P95C / R178C and F98C / R178C have high enzyme activity, ginsenoside Rb1 can be converted into CK, the conversion efficiency is respectively improved by 83.52% and 105.79% compared with that of wild beta-glucosidase, and the industrial application of enzymatic conversion of rare ginsenoside CK is promoted.

The invention discloses Dubosiella Newyorkens TZF-001 capable of producing beta-glucosidase and application of the Dubosiella Newyorkens TZF-001 in preparation of ginsenoside through fermentation and conversion, and belongs to the field of probiotic fermentation. The Dubosiella Newyorkens TZF-001 is preserved in the China Center for Type Culture Collection on December 15, 2025, and the preservation number of the Dubosiella Newyorkens TZF-001 is CCTCC (China Center for Type Culture Collection) NO: M20252889. According to the invention, ginsenoside Rb1 is used as a raw material, and Dubosiella Newyorkens TZF-001 with high yield of beta-glucosidase is used for fermentation and conversion of ginsenoside so as to prepare ginsenoside Rd. The ginsenoside Rb1 can be completely converted into Rd after fermentation, the dependence of a traditional method on acid-base hydrolysis, high temperature and high pressure and steam treatment is avoided, the reaction condition is mild, and the method has the advantages of being environmentally friendly, low in cost, high in product purity and the like. The method opens up a brand new technical path for green and low-cost preparation of ginsenoside Rd, and has remarkable innovativeness and popularization value in the field of industrialization of active ingredients of natural medicines.

The invention discloses a food for controlling postprandial blood sugar based on compound fermentation of plant raw materials and a preparation method of the food, and relates to the technical field of fermentation processing of functional foods and natural products. The sugar-controlling food contains plant raw material composite ferment powder, and a preparation method of the ferment powder comprises the following steps: S1, mixing mulberry leaf powder, buckwheat powder and soybean powder in proportion, and adding water to prepare a mixture with the water content of 55-60%; s2, inoculating a sugar-inhibiting eurotium cristatum liquid into the mixture, and performing solid-state fermentation to obtain a first fermented material; s3, inoculating the first fermented material with a lactobacillus plantarum bacterial liquid for continuous fermentation to obtain a second fermented material; and S4, carrying out enzyme deactivation, cooling, homogenizing, drying and crushing on the second fermentation material to obtain the plant raw material composite fermentation powder. The fermentation method disclosed by the invention not only can improve the content of hypoglycemic active ingredients and the inhibition rate of alpha-glucosidase, but also can reduce the bitterness of the active ingredients and balance the mouth feel.

The invention belongs to the technical field of plant fermented beverages, and particularly relates to a synergistically fermented composite plant beverage and a preparation method thereof. The preparation method comprises the following steps: sieving platycodon grandiflorum and liquorice, and then carrying out ultrasonic pretreatment and cold extraction to obtain a platycodon grandiflorum and liquorice cold extraction solution; adding pectinase and beta-glucosidase into the pear juice, and carrying out enzymolysis composite treatment, so as to obtain pear juice after enzymolysis; mixing the platycodon grandiflorum and liquorice cold extraction liquid and the pear juice subjected to enzymolysis to obtain a cold extraction mixed liquid, and then inoculating a combined strain for fermentation to obtain a fermented mixed liquid; and performing molecular distillation and concentration on the fermentation mixed solution, and then performing high-pressure carbon dioxide sterilization and photodynamic sterilization in sequence to obtain the product. According to the preparation method disclosed by the invention, the preparation time is effectively shortened, the energy consumption is reduced, the preparation efficiency is improved, and the prepared composite plant beverage is high in content of active ingredients and nutrient elements such as GABA, platycodin and the like, long in storage time and high in storage stability.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

The invention discloses a thiochromanone derivative containing a thiosemicarbazone structure and application of the thiochromanone derivative, relates to the technical field of medicines, and particularly relates to the technical field that the compound has better oxidation resistance and alpha-glucosidase resistance activity, the IC50 value of the compound 1-(7-chloro-4, 4-dioxothiochromanone-3-N-propyl) thiosemicarbazone to ABTS clearance activity is 2.86 [mu] g / mL, the IC50 value of the compound 1-(7-chloro-4, 4-dioxothiochromanone-3-N-propyl) thiosemicarbazone to alpha-glucosidase inhibition activity is 9.51 [mu] g / mL, and the effect is the best and is superior to that of a control agent vitamin E and acarbose. The thiochromanone derivative containing the thiosemicarbazone structure has a general formula shown in the specification,

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention provides a fruit juice fermentation inoculant, fermented guava juice and a preparation method, and belongs to the technical field of fermented beverages. The invention provides a compound fermentation inoculant capable of fermenting fruit juice, the compound fermentation inoculant is composed of lactobacillus plantarum and lactobacillus fermentum, the fermentation inoculant is applied to fermentation of guava fruit juice, beta-glucosidase secreted in the fermentation process can hydrolyze combined polyphenol substances in guavas, and then the guava fruit juice is fermented. The antioxidant is converted into high-activity antioxidants such as quercetin and gallic acid in a free state, so that the content of free phenol is increased by about 2 times, and the scavenging rate of DPPH free radicals is increased by more than 40%. The fermentation is carried out in an oxygen-free environment, the retention rate of vitamin C can reach 80% or above, the low pH value at the fermentation end point and organic acid jointly inhibit pathogenic microorganisms, and the product stability is guaranteed. Therefore, the guava juice prepared from the compound fermentation inoculant maximally releases the natural nutritional potential of guavas.

The invention provides a pseudoxanthomonas wenibertii strain TY3-10 and application thereof in preparation of rare ginsenoside, and belongs to the technical field of biological medicine. The preservation number of the pseudoxanthomonas wenibertii strain TY3-10 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35238. The pseudoxanthomonas wenibertii The strain TY3-10 has the characteristic of producing beta-glucosidase and beta-xylosidase, and can be used for converting various ginsenosides and notoginsenoside into rare ginsenosides F2, Rd2, Fd, C-Mc1 and Rh1. The invention further provides a method for preparing the rare ginsenoside, and ginsenoside is subjected to a conversion reaction under the action of the pseudoxanthomonas wenibertii strain TY3-10 to obtain the rare ginsenoside. The invention provides a new process for preparing the rare ginsenoside, and has a wide application prospect.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention discloses a method for retaining flavor of plum-leaf crab ice wine. The method comprises the following steps: 1, selecting red-pulp malus spectabilis fruits and eight-edge malus spectabilis fruits, washing, and draining with a vibrating screen until the surface moisture is less than or equal to 5%; 2, denucleating, taking pulp, pulping and sieving to obtain plum-leaf crab normal juice; 3, beta-glucosidase is added; 4, adding modified pectin microspheres, and carrying out three-stage cooling to obtain frozen plum-leaf crab normal juice; 5, slowly unfreezing, inoculating low-temperature-resistant saccharomyces cerevisiae liquid, and carrying out low-temperature segmented fermentation; 6, ageing; and 7, adding potassium tartrate, continuing for 2-3 months, filtering, and clarifying until the turbidity is less than or equal to 2NTU. The modified pectin microspheres with the adsorption-slow release function are added, terpene and ester flavor precursors in the plum-leaf crab are adsorbed in the fermentation period, the volatilization and hydrolysis rate of flavor substances in the fermentation process is reduced, especially the retention rate of plum-leaf crab characteristic fruity substances such as ethyl caprylate and ethyl caprate can be increased, and after being degraded, the modified pectin microspheres can be used for degrading the plum-leaf crab characteristic fruity substances. And the components are released back into the wine body.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

The present application relates to a kind of chalcone amide alpha-glucosidase inhibitors and its preparation and application, the inhibitor structural formula is: preparation method specifically is (1) after 2-hydroxyacetophenone, sodium hydroxide and methyl p-formylbenzoate are reacted, intermediate in formula (a) is obtained after post-processing;(2) intermediate in formula (a) is dissolved in organic solvent and potassium hydroxide is reacted, after reaction, intermediate in formula (b) is obtained after post-processing;(3) intermediate in formula (b) and substituted aniline are dissolved in organic solvent, after reaction, the inhibitor shown in formula (c) is obtained after post-processing.Compared with prior art, the compound of the present application is novel, and experiments show that it has good alpha-glucosidase inhibitory activity, and can be used for preparing alpha-glucosidase activity inhibiting drug.

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

The application discloses a preparation method of ethanol extract of Drynaria Rhizome and application thereof. The method comprises the following steps: crushing and sieving Drynaria Rhizome, adding ethanol, reflux extraction, combining filtrates, vacuum concentration and drying, and thus the ethanol extract of Drynaria Rhizome is obtained. The in-vitro alpha-glucosidase enzyme inhibition experiment shows that the ethanol extract of Drynaria Rhizome has a significant inhibitory effect on alpha-glucosidase enzyme, and the IC 50 is about 142.68 μ g / mL. Therefore, the ethanol extract of Drynaria Rhizome can be used for preparing a hypoglycemic drug, health care product or functional food, and has a wide application prospect.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

This invention discloses a method and product for preparing Pien Tze Huang herbal wine fermented on Cordyceps militaris culture medium, belonging to the field of health wine brewing technology. This method uses Cordyceps militaris culture medium (SCM) and Pien Tze Huang herbal wine (GFE) as raw materials, and prepares a health wine with hypoglycemic and antioxidant effects through a complex fermentation process including raw material pretreatment, enzymatic hydrolysis, yeast activation, anaerobic fermentation, and post-treatment. It aims to solve the problems of low raw material utilization, low active ingredient content, and insufficient antioxidant activity in existing technologies. Pretreatment employs ultrasonic extraction. Fermentation conditions are anaerobic fermentation at 28℃ for 18 days, with 3% sugar supplementation every 2 days, controlling the alcohol content at 6%-10% vol, and preferably using an SCM:GFE ratio of 2:1. The resulting product has an alcohol content of 8% vol, is rich in total phenols and total flavonoids, has an α-glucosidase inhibition rate of 82.6%, a DPPH free radical scavenging rate of 78.3%, and a FRAP value of 82.6 mM Fe. 2+ Equivalents / mL, with a harmonious flavor and significant health benefits, provide an effective way for the comprehensive development of Cordyceps militaris and Pien Tze Huang.

The invention provides a whitening and spot-fading skin care composition and a preparation method thereof, and belongs to the technical field of whitening and skincare, and the composition comprises modified glabridin, a nicotinamide-tranexamic acid co-coating, an alhagi sparsifolia flower extract and an artemisia desertorum seed extract. The modified glabridin is obtained by modifying glabridin with tocopherol succinate, polyethylene glycol and artemisia desertorum seed gum. The nicotinamide-tranexamic acid co-coating is obtained by coating a mixture of nicotinamide and tranexamic acid with sodium alginate and chitosan hydrochloride. The alhagi sparsifolia extract is obtained by carrying out common enzymolysis on alhagi sparsifolia dry powder through compound enzyme and glucosidase and purifying through a mixed filler adsorption column, and the artemisia desertorum seed extract is obtained by fermenting artemisia desertorum seed powder through aspergillus oryzae, removing impurities through ADS-7 resin and purifying through a polyamide resin column. The effective components are prepared by adopting a special preparation and purification method and are matched according to a specific ratio for use, and the whitening effects of inhibiting tyrosinase, blocking melanin generation, scavenging free radicals and the like are achieved through multi-effect synergism.