92 results about "Alglucerase" patented technology

The application discloses a preparation method of ethanol extract of Drynaria Rhizome and application thereof. The method comprises the following steps: crushing and sieving Drynaria Rhizome, adding ethanol, reflux extraction, combining filtrates, vacuum concentration and drying, and thus the ethanol extract of Drynaria Rhizome is obtained. The in-vitro alpha-glucosidase enzyme inhibition experiment shows that the ethanol extract of Drynaria Rhizome has a significant inhibitory effect on alpha-glucosidase enzyme, and the IC 50 is about 142.68 μ g / mL. Therefore, the ethanol extract of Drynaria Rhizome can be used for preparing a hypoglycemic drug, health care product or functional food, and has a wide application prospect.

The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of natural product extraction. The method comprises the following steps: puffing treatment of soybean meal, wall breaking treatment in a high-voltage electric field; acid precipitation, collection of supernatant; after ultrasonic oscillation of the supernatant, a composite enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after filtration of the material liquid, the material liquid is loaded onto a macroporous resin column for adsorption, and after completion of the adsorption, effluent is collected; the effluent is purified and concentrated to obtain soybean oligosaccharides; an ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by ethanol and dried to obtain soybean saponins; the composite enzyme comprises amylase and beta-glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main solvent, the consumption of organic solvents and the pollution to the environment are reduced, and the unit process water consumption, unit energy consumption and unit cost are reduced.

This invention discloses a method and product for preparing Pien Tze Huang herbal wine fermented on Cordyceps militaris culture medium, belonging to the field of health wine brewing technology. This method uses Cordyceps militaris culture medium (SCM) and Pien Tze Huang herbal wine (GFE) as raw materials, and prepares a health wine with hypoglycemic and antioxidant effects through a complex fermentation process including raw material pretreatment, enzymatic hydrolysis, yeast activation, anaerobic fermentation, and post-treatment. It aims to solve the problems of low raw material utilization, low active ingredient content, and insufficient antioxidant activity in existing technologies. Pretreatment employs ultrasonic extraction. Fermentation conditions are anaerobic fermentation at 28℃ for 18 days, with 3% sugar supplementation every 2 days, controlling the alcohol content at 6%-10% vol, and preferably using an SCM:GFE ratio of 2:1. The resulting product has an alcohol content of 8% vol, is rich in total phenols and total flavonoids, has an α-glucosidase inhibition rate of 82.6%, a DPPH free radical scavenging rate of 78.3%, and a FRAP value of 82.6 mM Fe. 2+ Equivalents / mL, with a harmonious flavor and significant health benefits, provide an effective way for the comprehensive development of Cordyceps militaris and Pien Tze Huang.

The application provides a kind of complex hydrolytic enzyme and its preparation method, it is related to enzyme engineering technical field.The preparation method includes: aspergillus niger is inoculated in culture medium to carry out fermentation, and the fermentation liquor containing beta-glucosidase is collected;The fermentation liquor is subjected to solid-liquid separation to obtain beta-glucosidase preparation;Add alpha-L-arabinofuranosidase and cellobiohydrolase to obtain the complex hydrolytic enzyme.This technology uses specific content of sodium carboxymethyl cellulose and wheat bran to synergistically ferment, the activity and specificity of the main enzyme beta-glucosidase are greatly improved by nutritional induction and conformation simulation;Subsequently, alpha-L-arabinofuranosidase and cellobiohydrolase are compounded, the single enzyme limitation is broken and the feedback inhibition is removed, and the overall catalytic efficiency and conversion rate are significantly improved.

This invention discloses an anti-hair loss hair root mask, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss hair root mask comprises the following components by weight percentage: 4.0%~9.0% anti-hair loss composition; the anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase derived from Aspergillus niger, β-glucosidase derived from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract. The anti-hair loss hair root mask, its preparation method, and its application of this invention achieve a significant increase in raw material utilization and anti-hair loss efficacy through targeted enzymatic release of active ingredients; the innovative product form ensures stable enzyme activity and precise efficacy; it also has scalp oil control and soothing effects and is easily mass-produced industrially.

This invention discloses a yam component with α-glucosidase inhibitory activity, its application, and analytical identification methods, belonging to the field of medicinal chemistry. The yam component is (Z)-6-ethoxy-9,10-dihydroxyoctadecano-7-enoic acid, a novel compound extracted from yam, exhibiting excellent α-glucosidase inhibitory activity, thus showing promising application prospects and value in lowering blood sugar.

The application discloses a quinoa hexapeptide with a blood sugar lowering effect and application thereof, and belongs to the technical field of bioactive peptides. The amino acid sequence of the quinoa hexapeptide PGSPGR is Pro-Gly-Ser-Pro-Gly-Arg. The quinoa hexapeptide PGSPGR has alpha-glucosidase inhibitory activity and can effectively reduce blood sugar level, and therefore, can be applied to preparation of medicines or food for lowering blood sugar. The application further provides a polypeptide containing the peptide segment PGSPGR and a quinoa peptide rich in the polypeptide, and the polypeptide and the quinoa peptide are applied to preparation of medicines or food for lowering blood sugar, thereby providing a new scheme and theoretical basis for treatment of diabetes, and having good market prospect and application potential.

This invention relates to the field of organic synthesis technology, specifically to a polyhydroxypyrrolidone ether compound, its preparation method, and its application. The structure of the polyhydroxypyrrolidone ether compound is shown in formula (1), and each group in formula (1) has a specific selection; the stereoconfiguration of the carbon atoms at positions 2, 3, and 4 in formula (1) is "2R,3R,4R" or "2S,3S,4S". The polyhydroxypyrrolidone ether compound of this invention and its pharmaceutically acceptable salts exhibit excellent selectivity and strong inhibitory effects on β-glucosidase, and are suitable for the prevention or treatment of Gaucher's disease and Parkinson's syndrome. Formula (1), Formula (2).

This invention discloses an anti-hair loss composition, its preparation method, and its application, relating to the field of cosmetic technology. The anti-hair loss composition comprises the following raw materials: plant extracts, enzyme activators, and a reconstitution system; the enzyme activator is selected from at least one of the following: β-glucosidase from Aspergillus niger, β-glucosidase from almonds, cellulase complex enzyme, malt extract, and citrus fruit fermentation broth; the plant extract includes Platycladus orientalis leaf extract; the reconstitution system includes a moisturizing stabilizer, a chelating agent, a buffer system, and deionized water. The anti-hair loss composition, its preparation method, and its application of this invention, through targeted enzymatic hydrolysis technology, efficiently convert inactive glycoside precursors in Platycladus orientalis leaf extract into free flavonoids with targeted anti-hair loss activity, significantly improving raw material utilization and active ingredient yield, achieving a technological breakthrough from "passively adding raw materials" to "actively generating active substances."

This invention application provides compositions containing red yeast rice pigment and their applications, compositions with α-glucosidase inhibitory activity and their applications, and further, their application in α-glucosidase inhibitors, belonging to the technical field of natural active compounds inhibiting α-glucosidase. The compositions of this invention comprise one or both of red yeast rice pigment and red yeast rice pigment: wherein the molar ratio of acarbose to red yeast rice pigment is 400:400-600:300; the molar ratio of acarbose to red yeast rice pigment is 400:200-600:150; and the molar ratio of red yeast rice pigment to red yeast rice pigment is 150:400-200:300. The compositions involved in this invention have a significant synergistic inhibitory effect on α-glucosidase, superior to using the above compounds alone, and can achieve reduced drug dosage and reduced drug side effects.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The application discloses a composition containing burdock polysaccharide for reducing blood sugar of type 2 diabetes patients and a preparation method and application thereof, and belongs to the technical field of medicine and food. The composition contains burdock polysaccharide, chitooligosaccharide, chlorogenic acid, epicatechin, resveratrol, ginsenoside Rd, and vitamin D, vitamin B1, vitamin B6, vitamin B12, magnesium glycinate and thaumatin protein. The application takes burdock polysaccharide as a core long-acting component, takes chitooligosaccharide as a short-acting synergistic component, constructs a long-short-acting synergistic blood sugar reducing system, and realizes the organic combination of rapid blood sugar reduction and stable blood sugar control. Experiments show that the composition can significantly reduce the fasting blood sugar of type 2 diabetes model rats, improve insulin resistance and glucose tolerance, reduce blood lipid and inflammatory factor levels, and reduce oxidative stress damage. The alpha-glucosidase inhibitory activity and in-vivo comprehensive blood sugar reducing effect of the composition are better than those of single burdock polysaccharide, and the composition has good taste and is suitable for long-term use of diabetes patients.

This invention discloses a high-efficiency protein expression system, its construction method, and its applications. The system includes a heterozygous promoter activator and a heterozygous promoter. The heterozygous promoter activator consists of the DNA-binding protein TetR, a linker peptide, and the transcription activator Mit1-Δ60-96. The heterozygous promoter consists of the protein-binding sequence TetO, a spacer sequence, and a basal promoter. The linker peptide is (GGGGS)×2, the spacer sequence is TCGCGCGC, and the basal promoter is P. AOX1 The core promoter of this invention. In the absence of a heterozygous promoter activator, the target protein gene initiated by the heterozygous promoter is expressed very weakly; in the presence of a heterozygous promoter activator, the target protein gene is expressed efficiently. This invention's system has good applicability and scalability, and the expression intensity of various target proteins, including xylanase, phytase, and glucosidase, is significantly higher than that of commonly used P. AOX1 Promoter.

This invention provides a type of yeast called *Saccharomyces clavatum*, characterized in that the *Saccharomyces clavatum* is: *Saccharomyces clavatum* (… Saccharomycopsis fibuligera AMCC 32248, preservation number CCTCC NO: M 20251908. Enzyme activity phenotypic screening, carbon source utilization analysis, and quantitative enzyme activity detection showed that this strain possesses highly efficient β-glucosidase activity; further analysis of key genes related to β-glucosidase... BGL1 Specific functional sequences were extracted and identified. Enzyme production conditions were optimized using response surface methodology, resulting in a significant increase in enzyme activity compared to before optimization. In a simulated fermentation system, this strain significantly promoted the production of specific acids, esters, aldehydes, alcohols, and other volatile flavor compounds. Its enzyme production characteristics and flavor regulation capabilities provide a new technical solution for addressing issues such as low hydrolysis efficiency of glycosides and insufficient flavor release in fermentation systems, showing potential applications in the food, brewing, feed, and pharmaceutical industries.

This invention discloses a functional oligosaccharide syrup prepared using crystalline glucose mother liquor, belonging to the field of comprehensive utilization of crystalline glucose mother liquor. Using crystalline glucose mother liquor as a substrate, this invention prepares an oligosaccharide syrup mainly composed of gentiosaccharides and maltodextrins through a combination of glucosidase catalysis and yeast fermentation. In vitro fermentation experiments have demonstrated that it can significantly increase the content of short-chain fatty acids in the product and promote the proliferation of beneficial intestinal bacteria such as Bifidobacteria and Lactobacillus, showing potential as a novel prebiotic. The product of this invention has high added value, and the preparation process is simple, facilitating industrial application. It provides a green, low-carbon, and economically beneficial new method for the comprehensive utilization of crystalline glucose mother liquor.

The application provides a beta-glucosidase mutant and application thereof in production of ginsenoside Rg3, and belongs to the technical field of genetic engineering.The application provides a beta-glucosidase mutant, and the amino acid sequence is shown as SEQ ID NO:1.The application screens a mutant capable of catalyzing the directional conversion of ginsenoside Rb1 into ginsenoside 20(S)-Rg3 through site-directed mutagenesis.Compared with a wild type, the mutant has a mutation site K715G, and the yield of Rg3 produced by enzymolysis of Rb1 is 585.95 muM, while the yield of the wild type is only 441.22 muM, and the efficiency of the directional conversion of ginsenoside Rb1 into ginsenoside 20(S)-Rg3 is significantly improved.

The application discloses a strain of Lecythophora ramosa LicG6 and application of the strain in solid-state fermentation of Pu'er tea, and belongs to the field of industrial microbial resource development and tea deep processing technology. The Lecythophora ramosa LicG6 is preserved in the China General Microbiological Culture Collection Center on June 14, 2024, and the address is No. 1, Xibei Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 41370. The application inoculates the Lecythophora ramosa LicG6 strain with double-enzyme synchronous secretion capacity, namely, simultaneously high expression of beta-glucosidase and polyphenol oxidase, into a solid-state fermentation system of Pu'er tea, realizes flower fragrance development by continuously hydrolyzing and releasing glycoside aroma precursor driven by beta-glucosidase under the condition of temperature control in stages, realizes red soup color transformation by oxidizing and polymerizing catechin driven by polyphenol oxidase, and thus, the two quality targets of "flavor enhancement" and "color transformation" are achieved simultaneously in the same fermentation process.

The application belongs to the technical field of medical intermediates, and discloses application of a cholesterols derivative in preparation of a diabetes drug. It is found by the application that the cholesterols derivative has good alpha-glucosidase inhibiting activity by using an in-vitro pharmacological activity model for evaluation, and has potential to be developed into a new type of diabetes prevention or treatment drug.

The application discloses methyl phenolic compounds, a preparation method and application thereof. Six new methyl phenolic compounds are separated from the skin of eleocharis tuberosa, and the compounds all show good inhibitory activity on alpha-glucosidase, and are stronger than the clinical first-line drug acarbose. The inhibitory activity of compound 3 is the best, enzyme kinetics research shows that the compound 3 plays a role as a reversible mixed inhibitor, ADME predicts that the compound 3 has good drug properties, including high gastrointestinal absorption and no CYP450 enzyme inhibition. The cytotoxicity experiment shows that the six compounds have no obvious growth inhibition on human embryonic kidney cells HEK293 at a concentration of 100 muM, and have good safety. The compounds can be combined with a pharmaceutically acceptable carrier to form a pharmaceutical composition, and are used for preparing a health care product for reducing blood sugar or a drug for treating diabetes. The preparation method is simple, easy to operate, suitable for industrial production, and is helpful to the high-value reuse of the skin of eleocharis tuberosa.

The application discloses a compound cereal powder for controlling blood sugar and reducing blood sugar, a preparation method and application thereof. The compound cereal powder comprises, in percentage by weight, 10-30% of soybean dietary fiber, 10-25% of resistant dextrin, 5-20% of soybean protein isolate powder, 5-15% of oat powder, 5-15% of black bean powder, 3-12% of purple sweet potato powder, 3-12% of oatmeal, 0.5-5% of poria cocos powder, 0.5-5% of yam powder, 0.5-5% of honeysuckle powder, 1-8% of konjac powder, 0.5-5% of medlar powder, 0.5-5% of mulberry leaf powder, 0.5-5% of rhizoma polygonati powder, compound vitamins, calcium carbonate, zinc gluconate and sodium carboxymethyl cellulose. The preparation method comprises the following steps: low-temperature baking and curing cereal raw materials, uniformly mixing by a three-dimensional mixer, screening and sterilizing, and sterilely sub-packaging. The compound cereal powder controls blood sugar and reduces blood sugar through multiple mechanisms such as a dietary fiber physical barrier, alpha-glucosidase inhibition, improvement of insulin resistance and supplement of trace elements, is comprehensive in nutrition, good in taste, and is suitable for people with diabetes and pre-diabetes as a meal replacement or a functional food for assisting blood sugar reduction.

This invention provides *Pediococcus lactis* SHI03 and its application in alleviating insulin resistance and type 2 diabetes, belonging to the field of microbial technology. This invention provides *Pediococcus lactis* SHI03, with accession number CCTCC No: M 20242686, and confirms that this strain possesses in vitro α-glucosidase inhibitory activity, exhibiting excellent probiotic and hypoglycemic effects. This invention further provides microbial products containing *Pediococcus lactis* SHI03, including *Pediococcus lactis* SHI03 fermentation broth, *Pediococcus lactis* SHI03 fermentation supernatant, and *Pediococcus lactis* SHI03 cells, and their applications in alleviating high-fat diet-induced insulin resistance and reducing fasting blood glucose in type 2 diabetic mice.

This invention belongs to the field of genetic engineering and biotechnology, specifically relating to a β-glucosidase PO3 for the conversion of rare ginsenosides, its preparation method, and its applications. This invention obtains β-glucosidase PO3 by PCR amplification using genomic DNA of *Xanthomonas pseudoepiplo* TY3-10 as a template. The results of the examples show that this enzyme can not only convert total ginsenosides into rare ginsenosides F2, C-O, C-Mx1, and C-Mc1, but also convert ginsenoside Rb1 into F2, ginsenoside Rb2 into C-O, ginsenoside Rb3 into C-Mx1, and ginsenoside Rc into C-Mc1; it also exhibits excellent catalytic performance and high expression efficiency, possessing extremely high application value and commercial prospects.

The application belongs to the technical field of biology, and particularly relates to two polypeptides with alpha-glucosidase inhibitory activity and application thereof. The application discloses the following two polypeptides with alpha-glucosidase inhibitory activity: polypeptide EGEPKLP and polypeptide TPELKL. The two polypeptides provided by the application both have strong alpha-glucosidase inhibitory activity, and have the advantages of safety, no toxic side effect, easy absorption and the like, and can be used in blood glucose lowering products (including blood glucose lowering drugs).

This invention discloses a high-efficiency protein expression system, its construction method, and its applications. The system includes a heterozygous promoter activator and a heterozygous promoter. The heterozygous promoter activator consists of the DNA-binding protein TetR, a linker peptide, and the transcription activator Mit1-Δ60-96. The heterozygous promoter consists of the protein-binding sequence TetO, a spacer sequence, and a basal promoter. The linker peptide is (GGGGS)×2, the spacer sequence is TCGCGCGC, and the basal promoter is P. AOX1 The core promoter of this invention. In the absence of a heterozygous promoter activator, the target protein gene initiated by the heterozygous promoter is expressed very weakly; in the presence of a heterozygous promoter activator, the target protein gene is expressed efficiently. This invention's system has good applicability and scalability, and the expression intensity of various target proteins, including xylanase, phytase, and glucosidase, is significantly higher than that of commonly used P. AOX1 Promoter.

Provided is a simple and specific means for detecting Listeria monocytogenes and / or Listeria ivanovii. A culture medium for detecting Listeria monocytogenes and / or Listeria ivanovii contains the following components (A), (B), and (C): (A) a chromogenic substance that develops color by being decomposed with β-glucosidase or a fluorogenic substance that emits fluorescence; (B) a chromogenic substance that develops color by being decomposed with phosphatidylinositol-specific phospholipase C or a fluorogenic substance that emits fluorescence; and (C) a sugar.

This invention discloses a reagent kit and method for the joint detection of glucose, cellobiose, and xylose in cellulose hydrolysate, belonging to the field of biomass conversion and analytical detection technology. The method includes the following steps: setting up three types of detection channels: a glucose oxidase detection channel that specifically responds to the glucose component; a β-glucosidase-glucose oxidase combined detection channel that responds to the total signal of glucose and cellobiose; and a pyranose oxidase detection channel that simultaneously responds to the combined signals of glucose, cellobiose, and xylose. Enzymatic colorimetric reactions are performed on the cellulose hydrolysate sample and the multi-component gradient standard solution in the three types of detection channels, respectively. A ternary linear calibration model is constructed based on the absorbance data of the three types of channels of the multi-component gradient standard solution. This method provides a rapid, low-cost, and highly specific detection method for glucose, cellobiose, and xylose in cellulose biomass hydrolysate, and has practical application value.

Pompe disease is an autosomal recessive lysosomal storage disorder caused by mutations in the gene encoding acid alpha-glucosidase (GAA). This disclosure provides engineered acid alpha-glucosidase polypeptides, recombinant polynucleotides encoding engineered acid alpha-glucosidase polypeptides, and methods for using engineered acid alpha-glucosidase polypeptides and recombinant polynucleotides for therapeutic purposes. This disclosure provides acid alpha-glucosidase polypeptides engineered to have improved properties, in particular, compared to naturally occurring human acid alpha-glucosidase.

This invention discloses a method for the targeted conversion and synthesis of rare ginsenosides Rg3 and Rh2. Specifically, fresh Panax notoginseng leaves are microwave-dried for 1-3 minutes to inactivate their endogenous β-glucosidase. The dried Panax notoginseng leaves are extracted with anhydrous methanol, and the extract is resuspended in an acetate-sodium acetate buffer solution after drying. α-arabinofuranase, β-xylosidase, and β-glucosidase are added to the resuspended solution, and an enzymatic reaction is carried out at 35-40℃ to directionally convert the original ginsenosides into rare ginsenosides Rg3 and Rh2. This method can achieve the inactivation of endogenous enzymes and the stable retention of original saponins in Panax notoginseng leaves in a very short time. Compared with the natural drying combined with exogenous enzymes, the conversion ratios of Rg3 and Rh2 are increased by 3.14 and 2.4 times, respectively. Compared with fresh Panax notoginseng leaves, the contents of Rg3 and Rh2 are increased by 20.6 and 8.7 times, respectively. This method is suitable for the high-value utilization of Panax notoginseng leaf waste resources and the large-scale preparation of rare saponins.

This invention provides a microbial preparation for enhancing the aroma and quality of low-grade tobacco leaves and its preparation method, belonging to the interdisciplinary field of microbial engineering and tobacco processing technology. Bacillus subtilis is inoculated into a fermentation medium containing low-grade tobacco leaf powder, using the powder as the core inducer for directional fermentation. Combined with staged fermentation regulation, the metabolic flow of Bacillus subtilis is directionally trained to produce a complex enzyme system highly matched to the degradation needs of tobacco leaves. Compared with conventional fermentation, this invention significantly enhances the activities of protease, amylase, and β-glucosidase. This complex enzyme system can simultaneously degrade proteins and starches in low-grade tobacco leaves and hydrolyze glycosidic bonds to release latent aroma substances. This invention is low-cost, achieves internal circulation within the tobacco industry chain, and is environmentally friendly.

The present invention aims to provide a saccharide composition suitable for a cyclic-tetrasaccharide-containing starch syrup which has low viscosity, low water activity, low coloration property, and low calorie content, and which is unlikely to cause precipitation of crystals of saccharides during storage, and to provide a use of the composition and a production method for the composition. The object is achieved by providing a cyclic-tetrasaccharide-containing saccharide composition having the following characteristics (1) to (3): (1) the saccharide composition includes a branched cyclic tetrasaccharide in addition to the cyclic tetrasaccharide, wherein the content of the cyclic tetrasaccharide with respect to the total solid content of the saccharide composition obtained by allowing glucoamylase and α-glucosidase to act on the above saccharide composition is 38% by mass or higher, on a dry solid basis; (2) the ratio of α-1,4-linked glucose in the total glucose residues constituting the saccharide composition in methylation analysis is over 9% and 15% or lower, and (3) the ratio of α-1,4,6-linked glucose in the total glucose residues constituting the saccharide composition in methylation analysis is less than 6%; and providing a use of the composition and a production method for the composition.