646 results about "Alglucerase" patented technology

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The invention provides an ultrasonic-assisted red pomelo polyphenol extraction method and application. The polyphenol component in the red pomelo is extracted through the following steps: firstly, performing activation pretreatment on red pomelo dry powder by adopting a low-temperature plasma device, and destroying a cell wall structure so as to improve the accessibility of combined polyphenol; then cellulase, pectinase, hemicellulase and beta-glucosidase are added into a biodegradable deep eutectic solvent to synergistically hydrolyze the cell wall polysaccharide, and combined polyphenol is released; then applying pulse type ultrasonic wave to assist extraction under a low-temperature condition, so that polyphenol is fully dissolved out and enters the eutectic solvent; and finally, carrying out solid-liquid separation to obtain the red pomelo polyphenol extract. Compared with a conventional heat extraction method, the method has the advantages that the extraction rate of the polyphenol of the red pomelo is remarkably increased at a lower temperature, the degradation of the polyphenol is effectively avoided, and the content of the total polyphenol and the antioxidant activity are increased; the method is green and environment-friendly, and can be applied to the health industry as an antioxidant.

The invention discloses a camel blood polypeptide and application thereof in preparation of an alpha-glucosidase inhibitor, a series of polypeptide products are separated from camel blood, and it is found that the camel blood polypeptide with the amino acid sequence being YFKI, DPPTMEL, DKPFGPDL or SEPSGTAFG has the remarkable alpha-glucosidase inhibitory activity; the compound can be applied to preparation of drugs for treating diseases caused by abnormal activity of alpha-glucosidase.

The invention belongs to the technical field of biology, and relates to a combined method for improving the taste of cerasus humilis clear juice and increasing the content of available calcium and zinc, which comprises the following operation steps: S1, removing impurities from cerasus humilis fruits, cleaning, denucleating and crushing to obtain cerasus humilis pulp; s2, performing primary enzymolysis on the cerasus humilis fruit pulp to obtain primary enzymolysis cerasus humilis fruit pulp; s3, fermenting the cerasus humilis fruit pulp to obtain fermented cerasus humilis fruit pulp; s4, performing secondary enzymolysis on the fermented cerasus humilis fruit pulp to obtain secondary enzymolysis cerasus humilis fruit pulp; and S5, filtering, sterilizing and filling the secondary enzymolysis cerasus humilis pulp to obtain a cerasus humilis clear juice product. The combined method of high-speed dispersion machine release, pectinase enzymolysis, issatchenkia terricola fermentation and joint enzymolysis of cellulase, beta-glucosidase and alpha-rhamnosidase is utilized, the product taste is effectively improved while the yield of the cerasus humilis clear juice product is increased, and the cerasus humilis clear juice product is palatable in sourness and sweetness, clear, transparent and bright, full in flavor and suitable for large-scale production. And the contents of calcium and zinc are increased by more than 65% and 59%. The product has good calcium and zinc supplementing effects.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses an enzyme immobilization method based on charge directed crosslinking, and belongs to the technical field of enzyme immobilization. The method sequentially comprises the following steps: reacting an enzyme with a modifier containing an anionic group to generate an electronegative modified enzyme; dissolving a protonated amino carrier in an acidic buffer solution to form a first solution, and dispersing a dissociation anion group-containing carrier and a modification enzyme in an alkaline buffer solution to form a second solution; mixing the two solutions, and constructing a gel network immobilized enzyme through electrostatic crosslinking; and finally, strengthening the gel network by using a multivalent metal ion solution. Directional anchoring of the enzyme in the gel is achieved through charge matching, the network stability and the enzyme microenvironment are synchronously optimized in combination with metal ion coordination, use of a covalent cross-linking agent is avoided in the whole process, conformation damage of an enzyme active center is avoided, the activity retention rate, the operation half-life period and the substrate mass transfer efficiency of the immobilized enzyme are remarkably improved, and the immobilized enzyme has good application prospects. The method is suitable for efficient immobilization of industrial enzymes such as beta-glucosidase, and has high biocompatibility and industrial potential.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention discloses a composite double-protein hypoglycemic peptide as well as a preparation method and application thereof, and belongs to the technical field of animal and plant source double-protein active peptides. The method comprises the following steps: performing computer simulation enzyme digestion and virtual screening by utilizing bioinformatics to determine a protein raw material and protease, performing enzymolysis on animal and plant double proteins (soybean protein and casein) serving as raw materials by adopting neutral protease, and performing separation, purification and structural identification, thereby obtaining the protein. The protein peptide fragment with high alpha-glucosidase inhibitory activity is obtained by virtually screening molecular docking, and the amino acid sequences of the protein peptide fragment are shown as SEQ ID No: 6, SEQ ID No: 9 and SEQ ID No: 24. The compound double-protein peptide sequence provided by the invention has an inhibiting effect on the activity of alpha-glucosidase, can be used for preventing and treating diabetes mellitus, and can be used for long-term health care or treatment of people with impaired glucose tolerance or diabetics as a functional food ingredient.

The invention discloses a method for improving in-vivo and in-vitro antioxidant activity of pomegranate peel polyphenol extract through combined enzymolysis of tannase and beta-glucosidase, and relates to the technical field of food science and biology. The pomegranate peel polyphenol extract provided by the invention is obtained from pomegranate peel powder through solvent extraction. According to the present invention, tannase and beta-glucosidase are adopted to perform single or combined enzymolysis on the pomegranate peel polyphenol extract, and the determination on the in vitro antioxidant activity of the pomegranate peel polyphenol extract shows that the antioxidant activities of DPPH and ABTS after the combined enzymolysis are improved by 29.84% and 29.30% compared with the antioxidant activities of the untreated polyphenol extract; the UPLC-ESI-QTOF-MS is used for identifying and analyzing the components of the enzymatic hydrolysate, the content of macromolecular ellagic tannin is obviously reduced, and the content of micromolecular ellagic acid and gallic acid is increased. The enzymolysis polyphenol is applied to D-galactose induced oxidative stress mice, and the result shows that the oxidative damage relieving effect of the enzymolysis polyphenol on the mice is better than that of untreated polyphenol.

The invention relates to the technical field of ginsenoside, aims to solve the problem of low conversion rate of ginsenoside Rg3 prepared by adopting a microbial conversion method in the prior art, and particularly discloses beta-glucosidase and a preparation method thereof, and application of the beta-glucosidase in production of ginsenoside Rg3. The preparation method of the beta-glucosidase comprises the following steps: S1, inserting a beta-glucosidase gene into a pET vector to obtain a recombinant plasmid; s2, inoculating the recombinant plasmids into competent cells, culturing the competent cells in an antibiotic-free culture medium and a resistant culture medium in sequence to form monoclonal antibodies, performing IPTG (isopropyl-beta-d-thiogalactoside) induction culture to form monoclonal strains, and storing the monoclonal strains in glycerol to obtain glycerol bacteria; s3, glycerol bacteria are placed in an LB culture medium to be cultured and then transferred into a fermentation culture medium, the fermentation culture medium is placed in a fermentation tank to be fermented, then centrifugal separation, thallus collection and re-suspension are conducted, beta-glucosidase is obtained and used for decomposing ginseng total saponins to produce ginsenoside Rg3, and the conversion rate of main products can reach 98%.

The invention discloses a Coaerella coagulum-plant lactobacillus synergistic fermentation inoculant and a process for staged solid-state fermentation of American ginseng by using the same, and belongs to the technical field of biological fermentation. According to the invention, two strains, i.e., the plant lactobacillus and the hainerella coagulatum, are obtained by screening food-grade probiotic strains, and the ginsenoside conversion capability of the two strains is verified by utilizing culture. The invention also provides a probiotic fermentation process for improving the content of rare ginsenoside in American ginseng, namely, the staged synergistic fermentation of the Halidernia coagulum and the phytobacterium plantarum is utilized, so that the content of the rare ginsenoside in the American ginseng is improved; cellulase and hemicellulase secreted by the Haihdrikia coagulatum can decompose cellulose, lignin and the like in cell walls of the surface layer of the American ginseng raw material in the early stage of fermentation, so that ginsenoside in cells is fully released, and beta-glucosidase from different sources is utilized to synergistically convert prototype ginsenoside in the later stage of fermentation. The content of various rare ginsenosides in American ginseng can be remarkably increased through the solid-state fermentation process. Meanwhile, the solid-state fermentation process has the advantages of mild conditions, less wastewater treatment, environmental protection, low cost, process safety and the like, has important significance in improving the resource utilization of American ginseng and promoting the industrial development of American ginseng, and has relatively good practicability.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention discloses a method for improving the quality of a Tieguanyin tea extracting solution through segmented composite enzymolysis extraction, and belongs to the technical field of tea drink preparation. The method comprises the following steps: S1, performing low-temperature aroma extraction on Tieguanyin dry tea, and performing crushing so as to obtain crushed tea; s2, adding water and beta-glucosidase into the broken tea to carry out first-stage enzymolysis extraction, and separating to obtain tea aroma extract liquid and aroma-free tea residues; s3, water and composite hydrolase are added into the aroma-free tea residues for second-stage enzymolysis extraction, separation and filtration are performed, and a hydrolysis extraction solution is obtained; and S4, mixing the tea fragrance extract liquid with the hydrolysis extract liquid, cooling, centrifuging and filtering to obtain the Tieguanyin tea extract liquid. According to the method disclosed by the invention, the original flavor of the Tieguanyin is maintained, meanwhile, the fragrance and the mouth feel are remarkably improved, and the problems of fragrance loss and bitter taste caused by traditional high-temperature long-time extraction and cooked soup taste generated by tannase enzymolysis are avoided.

The invention discloses an agaric polypeptide VR5 with alpha-glucosidase inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the auricularia auricula polypeptide VR5 is VGMLR, and the auricularia auricula polypeptide VR5 can be prepared by a solid-phase synthesis method and an enzymolysis method (flavourzyme). The agaric polypeptide VR5 has the beneficial effects that experiments prove that the agaric polypeptide VR5 has very high alpha-glucosidase inhibitory activity, IC50 is equal to 34.96 mu g / mL, the agaric polypeptide VR5 can be used as an alpha-glucosidase inhibitor for preventing or treating alpha-glucosidase mediated diseases, and the agaric polypeptide VR5 can be used for preventing or treating alpha-glucosidase mediated diseases. Or as a hypoglycemic active component, the composition is added into auxiliary hypoglycemic functional food for auxiliary regulation of the blood sugar level of hyperglycemia people.

The invention discloses a marine-derived bioactive peptide with a blood sugar reducing effect, a preparation and application, and belongs to the technical field of bioactive peptides. The amino acid sequence of the bioactive peptide is at least one of FYP, FPF and YAPFPK. Three bioactive peptides with the blood sugar reducing effect are separated and purified from oysters through enzymolysis, ultrafiltration, gel chromatography, liquid chromatography-mass spectrometry and bioinformatics technologies. According to the present invention, chemical synthesis is performed according to the identified peptide fragment sequence, the hypoglycemic activity of the synthesized single peptide fragment and the compounded peptide fragment is detected, and the bioactive peptides have good alpha-glucosidase inhibition activity, such that the bioactive peptides have good application prospects in the preparation of the products with the hypoglycemic effect, and can be widely used in the preparation of the products with the hypoglycemic effect. And a foundation is laid for high value-added utilization of oysters and promotion of research, development and application of functional active peptides.

The invention relates to the technical field of antibacterial agents, in particular to a compound antibacterial agent of a plant polyphenol complex and a preparation method of the compound antibacterial agent. The compound antibacterial agent is prepared from the following raw materials in parts by weight: 20-30 parts of moutan bark extract, 20-30 parts of folium mori, 20-30 parts of a carrier, 10-20 parts of jerusalem artichoke extract, 10-20 parts of coptis chinensis extract, 2-4 parts of a tackifier and 1-3 parts of a microbial agent. According to the invention, paeonol in moutan bark, chlorogenic acid in mulberry leaves, berberine in coptis chinensis and inulin polyphenol in jerusalem artichoke can be combined with lipid of bacterial cell membranes, so that membrane permeability is increased, microbial metabolic enzymes are interfered, energy metabolism and biosynthesis are blocked, free radicals are removed, and antibacterial effects of other components are enhanced; folium mori is hydrolyzed through beta-glucosidase to release free polyphenol, gluconolactone adjusts the acid environment to promote polyphenol dissolution, and the antibacterial effect of the composite antibacterial agent is effectively improved.

The invention discloses a preparation method and application of a benzofuran component in eupatorium chinense. The benzofuran component is separated from an eupatorium chinense extract. The eupatorium chinense root extract is separated by using an extraction method, macroporous adsorption resin impurity removal, HW-40F gel column chromatography, high performance liquid chromatography and other methods to obtain the benzofuran compound. According to the present invention, the inhibition activity of the separated compound on the diabetes-related target alpha-glucosidase is tested, the experimental result shows that the compound has good inhibition activity on the alpha-glucosidase, and the molecular docking experiment shows that the compound has good binding energy with the target protein alpha-glucosidase. Therefore, the compound can be used for preparing drugs for preventing, delaying or treating alpha-glucosidase diseases, especially type II diabetes and obesity, or can be used as a lead compound of the drugs.

The invention belongs to the technical field of microorganisms and biological medicines, and particularly relates to marine-source lactobacillus plantarum HYC-1 with cholesterol lowering and alpha-glucosidase inhibiting activity and application of the marine-source lactobacillus plantarum HYC-1. The novel lactobacillus plantarum HYC-1 is screened from intestinal tracts and contents of marine fishes, the strain has efficient cholesterol lowering activity and alpha-glucosidase inhibition activity at the same time, and experimental results show that the in-vitro cholesterol lowering rate of lactobacillus plantarum HYC-1 fermentation liquor can reach 89.4%, the alpha-glucosidase inhibition rate can reach 93.5%, and the lactobacillus plantarum HYC-1 fermentation liquor can be used for preparing the cholesterol lowering agent. One bacterium has double effects and unique functions; besides, the strain shows excellent tolerance and high safety, so that the strain can be widely applied to food, health care products and medicines, especially to preparation and research and development of medicines with the efficacy of regulating blood fat and blood sugar.

The invention discloses preparation of immobilized alpha-glucosidase based on a magnetic metal organic framework (MOF) and application of the immobilized alpha-glucosidase in screening of natural enzyme inhibitors. According to the method, ZIF-90 is grafted on the surfaces of Fe3O4 magnetic nanoparticles modified by codeposition of polydopamine and polyethyleneimine, and alpha-glucosidase is synchronously encapsulated in situ in the growth process of the ZIF-90, so that the magnetic MOF immobilized alpha-glucosidase is successfully constructed. On the application level, an in-vitro enzyme inhibition activity evaluation system is constructed, multiple traditional Chinese medicine extracts are selected as research objects, and by means of the characteristic that the magnetic MOF immobilized alpha-glucosidase is easily separated from a reaction system, accurate screening of natural inhibitors or active small molecules is rapidly and efficiently achieved; and a novel screening method is provided for research and development of candidate drugs for diabetes mellitus. The method comprises the following steps: 1, preparing the magnetic MOF immobilized alpha-glucosidase and evaluating the performance of the magnetic MOF immobilized alpha-glucosidase; and 2, the prepared immobilized alpha-glucosidase is used for screening a natural enzyme inhibitor. Based on a magnetic MOF immobilized enzyme technology, the method provided by the invention has the characteristics of rapidness, high efficiency, accuracy, easiness in separation from a reaction system and reutilization of a target receptor alpha-glucosidase, is suitable for screening of alpha-glucosidase inhibitors in complex systems such as traditional Chinese medicines, and has a wide application prospect.

The invention relates to a multi-component ginseng extract containing ginseng polypeptide and a preparation method of the multi-component ginseng extract. The preparation method comprises the following steps: S1, preparing ginseng powder from a ginseng raw material; mixing the ginseng powder with an acidic buffer solution, and carrying out presoaking treatment and homogenate dissolution promoting treatment to obtain ginseng primary pulp; s2, mixing the wall-breaking enzyme system with the ginseng primary pulp, and performing acid wall-breaking treatment to obtain wall-broken primary pulp; mixing an invertase system with the wall-broken primary pulp, and carrying out enzymolysis synchronous glucoside conversion treatment to obtain a crude extract; s3, filtering and separating the crude extracting solution to obtain an extract containing the ginseng polypeptide; the pH values of the ginseng primary pulp, the wall-broken primary pulp and the crude extract are in a range of 4.0-6.0; the wall breaking enzyme system comprises pectinase and cellulase; the invertase system comprises beta-glucosidase and acid protease. The technical problems that in the prior art, a ginseng extract is complex in extraction process, multiple active ingredients are difficult to efficiently extract at the same time, the product quality is low, and the biological activity is poor are solved.

The technical scheme of the invention discloses black fungus protein capable of reducing blood glucose and resisting oxidation as well as a preparation method and application of the black fungus protein. The amino acid sequence of the black fungus protein is as shown in SEQ ID NO. 1. The protein which is separated and identified from the black fungus for the first time and has the effects of reducing blood glucose and resisting oxidation achieves the effect of reducing blood glucose through double inhibition of alpha-amylase and alpha-glucosidase, meanwhile, the protein has remarkable antioxidant activity, animal experiments prove that fasting blood glucose and body weight of a type II diabetes model mouse can be effectively reduced, and the protein has a good application prospect. The compound has high biological activity and safety, and can be used for developing functional products with hypoglycemic and antioxidant activity.

The invention relates to the technical field of functional food, and discloses a formula and a preparation technology of medicinal and edible walnut milk with a memory enhancing effect, and the formula comprises walnut kernels, medicinal and edible compound dry powder, purified water and a compound bacteria solution. The preparation process comprises the following steps: firstly, performing sequential enzymolysis on the medicinal and edible compound dry powder, which comprises two stages of cell wall disintegration and beta-glucosidase hydrolysis, so as to prepare a functional core pretreatment solution; secondly, when the walnut matrix is prepared, the homogenizing pressure is dynamically set and adjusted according to the real-time viscosity of the pretreatment liquid; and finally, mixing the two feed liquids, inoculating a compound bacteria liquid containing lactobacillus plantarum and bifidobacterium longum, and carrying out double-step temperature fermentation which comprises two stages which are sequentially carried out at different temperatures. Through organic combination of the processes, the dissolution level of functional components is effectively improved, and the physical stability and sensory flavor of the product are improved.

Provided are pharmaceutical formulations comprising a recombinant acid α-glucosidase, wherein the recombinant acid α-glucosidase is expressed in Chinese hamster ovary (CHO) cells and comprises an increased content of N-glycan units bearing one or two mannose-6-phosphate residues when compared to a content of N-glycan units bearing one or two mannose-6-phosphate residues of alglucosidase alfa; at least one buffer selected from the group consisting of a citrate, a phosphate and combinations thereof; and at least one excipient selected from the group consisting of mannitol, polysorbate 80, and combinations thereof, wherein the formulation has a pH of from about 5.0 to about 7.0. Also provided are methods of treating Pompe disease using these pharmaceutical formulations.

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to a beta-glucosidase mutant with high catalytic activity and application of the beta-glucosidase mutant. The sequence of the beta-glucosidase mutant is obtained by carrying out any one of the following mutations on an amino acid sequence as shown in SEQ ID No.1: respectively mutating proline at the 95th site and arginine at the 178th site into cysteine; phenylalanine at the 98th site and arginine at the 178th site are respectively mutated into cysteine. The obtained mutants P95C / R178C and F98C / R178C have high enzyme activity, ginsenoside Rb1 can be converted into CK, the conversion efficiency is respectively improved by 83.52% and 105.79% compared with that of wild beta-glucosidase, and the industrial application of enzymatic conversion of rare ginsenoside CK is promoted.

The invention relates to the technical field of nano biosensing, in particular to a cerium-based metal organic framework loaded gold nano-cluster composite material as well as a preparation method and application thereof. A fluorescent and colorimetric dual-channel sensing platform is formed by compounding AuNCs and Ce-MOF and is used for detecting alpha-glucosidase and screening an alpha-glucosidase inhibitor, the cerium-based metal organic framework loaded gold nano-cluster composite material catalyzes and oxidizes a colorless substrate ABTS to generate colored oxABTS, and the oxABTS has characteristic ultraviolet absorption at 410 nm, so that the detection of alpha-glucosidase and screening of an alpha-glucosidase inhibitor can be realized. And the fluorescence of the cerium-based metal organic framework loaded gold nano-cluster composite material can be efficiently quenched through an inner filtering effect. Alpha-glucosidase catalyzes hydrolysis of a substrate AAG to generate AA, the AA can inhibit oxidation of ABTS regulated and controlled by a cerium-based metal organic framework loaded gold nano-cluster composite material, and then reduction of oxABTS ultraviolet absorption and fluorescence recovery of ACM are caused, so that dual-channel detection of alpha-GLU is realized through dual-signal output of colorimetric and fluorescence signals.

The invention discloses an alpha-1, 4 glucosidic bond hydrolytic ability enhanced oligomerized 1, 6-glucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The oligomerized 1, 6-glucosidase mutant V219A / P227A disclosed by the invention also has the alpha-1, 4 glucosidic bond hydrolytic activity which is not possessed by a wild type enzyme on the basis of retaining the original alpha-1, 6 glucosidic bond hydrolytic activity, and the alpha-1, 4 glucosidic bond hydrolytic activity is improved by 267% compared with that of a single mutant V219A. The mutant V219A / P227A is applied to an enzymatic production process of glucose, maltodextrin is taken as a substrate, glucamylase and the mutant V219A / P227A are added for enzymatic conversion, and the final glucose yield reaches 98.0%; the mutant V219A / P227A is applied to regeneration of glucose from crystalline glucose mother liquor, and the final glucose yield reaches 95.9%, which is 9.35% and 5.15% higher than those of a wild type V219A and a single mutant V219A respectively. Therefore, the mutant is helpful for improving the substrate conversion rate and the product purity in the glucose production process, can be used for regenerating glucose from crystalline glucose mother liquor, and has higher industrial application value.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.