73 results about "Pancrelipase" patented technology

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention discloses a horse milk-derived small molecule peptide, the amino acid sequence of which is Met-Ser-Pro-Phe (MSPF), and the molecular weight of which is 480.571 Da. The small molecule peptide disclosed by the invention has the activity of inhibiting pancreatic lipase (PL), the inhibition rate on the pancreatic lipase is 61.44%, and the accumulation of the content of triglyceride (TG) in oleic acid-induced human liver cancer (HepG2) cells can be reduced, so that the small molecule peptide can be used for inhibiting the pancreatic lipase (PL). The small molecule peptide can be applied to preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, is simple to prepare, and is suitable for industrial production and market popularization and application.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention relates to the field of microbial fermentation food, and discloses application of lactobacillus plantarum, a prune fermentation product, a preparation method of the prune fermentation product and a prune fermentation strain. A strain of lactobacillus plantarum with better performance is obtained from the nature, the preservation number is CCTCC NO: M 20242280, and after the lactobacillus plantarum is used for fermenting the prune subjected to enzymolysis, compared with other fermentation strains and fermentation products without fermentation strains, the obtained fermentation product has the advantages that the fermentation yield is increased, and the yield is increased. The pancreatic lipase inhibition rate and the cholesterol micelle solubility inhibition rate are remarkably improved, and the compound shows extremely excellent activity of improving blood fat. The invention not only provides an effective, time-saving and energy-saving preparation process method of the prune fermented product, but also provides a wide foundation for further application of the prune.

The application relates to the technical field of microbial fermentation, and discloses a lily fermentation product for improving blood lipid, a preparation method and application thereof. Lily raw materials are taken as fermentation objects, and suitable lactobacillus plantarum fermentation strains are selected to ferment the lily raw materials to obtain a lily fermentation product. Compared with other fermentation products of lactobacillus plantarum, the lily fermentation product of the lactobacillus plantarum of CICC 25125 has more excellent pancreatic lipase inhibition capacity and has the effect of promoting and improving blood lipid levels, thereby providing a better basis for the application of the lily fermentation product in the food field.

The application discloses a small-molecule peptide from fermented mare milk, which has an amino acid sequence of His-Ala-Trp-Phe and a molecular weight of 559.67 Da. The small-molecule peptide has pancreatic lipase inhibitory activity, can be applied to the preparation of a medicine for reducing high blood fat, and is simple to prepare, suitable for industrialized production and market promotion and application.

The invention discloses a method for extracting tetrandrine in stephania tetrandra, which comprises the following steps: (1) mixing stephania tetrandra and ethanol, extracting, collecting filtrate, and removing ethanol to obtain stephania tetrandra extract; (2) mixing organic matters, inorganic salts and water to obtain an aqueous two-phase system; and (3) adding the stephania tetrandra extract into the aqueous two-phase system, and then carrying out ultrasonic treatment, shaking extraction and centrifugation to obtain an upper-phase system and a lower-phase system which contain tetrandrine. According to the method for extracting the tetrandrine in the stephania tetrandra, an aqueous two-phase extraction method and an ultrasonic technology are combined, the extraction rate of the tetrandrine in the stephania tetrandra is taken as a target, and an extraction and separation process of the tetrandrine in the stephania tetrandra extract is optimized by adopting the aqueous two-phase extraction method. The tetrandrine extracted by the ultrasonic-assisted aqueous two-phase method is high in yield and low in solvent residue. The extract obtained by the method has a good inhibition effect on pancreatic lipase.

The invention discloses a small molecule peptide which is derived from fermented horse milk, the amino acid sequence of the small molecule peptide is Phe-Ser-Pro-Phe, and the molecular weight of the small molecule peptide is 496.549 Da. The small molecule peptide disclosed by the invention has the activity of inhibiting pancreatic lipase (PL), the highest inhibition rate of the small molecule peptide to the pancreatic lipase is 58.58%, the accumulation of the content of triglyceride (TG) in oleic acid induced human liver cancer (HepG2) cells can be reduced, and the small molecule peptide can be used for preparing a medicine for treating liver cancer. The small molecule peptide disclosed by the invention can be applied to preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, and has the characteristics of simplicity in preparation and suitability for industrial production and market popularization and application.

The invention discloses a separation process of a pancreatic lipase inhibitor Lycrutheophylpropanoid J with a new structure in red Chinese wolfberry fruits and application of the pancreatic lipase inhibitor Lycrutheophylpropanoid J with the new structure in the red Chinese wolfberry fruits. The specific preparation process comprises the following seven steps: extraction, on-line pancreatic lipase inhibitor component recognition, hydrophilic water pressure preparation column coarse separation, reversed-phase high-pressure preparation column preparation, hydrophilic high-pressure preparation column preparation, gel filtration chromatography and in-vitro pancreatic lipase inhibition experiment. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by the invention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch material preparation is easy; in the preparation process, solvents and separation materials used by an extraction solvent, a hydrophilic preparative column, a reversed-phase preparative column, gel chromatography and a reversed-phase chromatographic column can be recycled.

The invention discloses a separation process of a pancreatic lipase inhibitor Lycrutheophylpropanoid H with a new structure in red Chinese wolfberry fruits and application of the pancreatic lipase inhibitor Lycrutheophylpropanoid H with the new structure in the red Chinese wolfberry fruits. The specific preparation process comprises the following seven steps: extraction, on-line pancreatic lipase inhibitor component recognition, hydrophilic water pressure preparation column coarse separation, reversed-phase high-pressure preparation column preparation, hydrophilic high-pressure preparation column preparation, gel filtration chromatography and in-vitro pancreatic lipase inhibition experiment. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by the invention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch material preparation is easy; in the preparation process, solvents and separation materials used by an extraction solvent, a hydrophilic preparative column, a reversed-phase preparative column, gel chromatography and a reversed-phase chromatographic column can be recycled.

The invention provides a fermentation product of seedless clausena lansium as well as a preparation method and application of the fermentation product, and the fermentation product is prepared by performing ultrasonic-assisted fermentation on seedless clausena lansium through fermentation lactobacillus mucus Q7 with the preservation number of GDMCC NO: 65149. The preparation method comprises the following steps: pretreating a seedless clausena lansium raw material, inoculating the seedless clausena lansium raw material with an activated bacterial solution of the strain, and fermenting under the optimal process conditions that the fermentation time is 52 hours, the inoculation amount is 7%, the fermentation temperature is 37 DEG C, the ultrasonic power is 100 W and the ultrasonic time is 63 minutes. According to the method, the active ingredients of the product are remarkably improved, the total phenol content reaches 4.70 mg GAE / g, and the pancreatic lipase inhibition rate reaches 96.30%. The fermentation product is rich in phenols, flavones and organic acids, has excellent antioxidant activity and lipid-lowering activity, and can be used for developing functional food, health care products or medicines.

The application discloses a mulberry leaf astringency removing treatment process and super-micro powder thereof. The process mainly uses a fermented coix as an astringency removing agent to remove the astringency of frost mulberry leaves in combination with three steaming and hot air drying methods. The application solves the problems of astringency and green smell of the mulberry leaves by using a relatively simple process, retains the unique taste and fragrance of the mulberry leaves, and adds unique alcohol and ester fragrance, so that the taste and smell of the mulberry leaves are rich in layers. The prepared astringency removed mulberry leaf super-micro powder has high retention rate of main nutritional components, has obvious inhibition effect on alpha-glucosidase and pancreatic lipase, and can be used as a food base to develop health food for reducing blood sugar and blood fat.

The invention relates to the technical field of functional food, in particular to a tartary buckwheat and roxburgh rose composition for regulating blood pressure and blood fat and a preparation method thereof. The tartary buckwheat and roxburgh rose composition comprises tartary buckwheat and roxburgh rose. The mass ratio of the tartary buckwheat to the roxburgh rose is 1: (1-3). In-vitro activity evaluation, cell experiments and animal experiments prove that flavonoid compounds of tartary buckwheat and active ingredients such as vitamin C of roxburgh rose in the composition generate a synergistic effect, so that the antioxidant capacity, the ACE inhibition rate, the cholesterol clearance rate and the pancreatic lipase inhibition rate can be remarkably improved, NO generation is effectively promoted, and the curative effect is good. The contents of TC, TG and LDL-L are reduced, the HDL-L level is increased, and the balance of vascular active substances is regulated. The raw materials are all medicinal and edible, are high in safety and free of side effects, provide a natural and efficient auxiliary conditioning scheme for chronic metabolic diseases such as hypertension and hyperlipidemia, and have a good industrialization prospect.

A novel polypeptide with double biological activities is obtained from sea cucumber intestines, and the sequence of the polypeptide is KADANNQWVDVK through high performance liquid chromatography purification and mass spectrometry analysis and identification. The in-vitro activity evaluation of a system shows that the polypeptide not only shows remarkable antioxidant capacity and can effectively remove ABTS free radicals, but also shows relatively strong pancreatic lipase inhibitory activity, and the IC50 value of the polypeptide reaches a remarkable level. The marine active peptide with double functions of oxidation resistance and metabolism regulation shows a good application prospect in the field of functional food and medicine development. The sea cucumber intestines are treated through an enzymolysis technology, low-molecular-weight polypeptides are separated through dialysis, de novo sequencing is carried out, and high-throughput polypeptides are synthesized. The polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is screened, and the polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is verified. According to the method, sea cucumber processing byproducts are fully utilized, the resource utilization rate is increased, a scientific basis is provided for high-value utilization of sea cucumber intestines, and meanwhile, a potential material basis is provided for developing natural antioxidants and peptides with lipid-lowering functions.

This invention discloses a method for screening pancreatic lipase inhibitory peptides based on machine learning, relating to the fields of bioinformatics and food science. The method includes the following steps: dataset construction and three-dimensional peptide structure characterization, statistical-based feature screening, outlier removal based on a dual-model consensus mechanism, feature recursive elimination and physicochemical significance optimization, data augmentation based on Gaussian noise injection, and final prediction model construction. This invention effectively solves the problems of insufficient feature representation, high noise interference, and weak model generalization ability in existing screening methods through multi-dimensional feature characterization, rigorous outlier cleaning, and data augmentation strategies for small samples. It can quickly and accurately screen highly active pancreatic lipase inhibitory peptides from massive peptide sequences, providing an efficient computational tool for the development of functional foods and lipid-lowering drugs.

The application belongs to the technical field of microorganisms, and discloses a bacillus amyloliquefaciens D189 with high yield of extracellular polysaccharide. The bacillus amyloliquefaciens D189 is preserved in China Center for Type Culture Collection on April 29, 2021, and the preservation number is CCTCC NO: M2021484. The bacillus amyloliquefaciens D189 provided in the application has high yield of extracellular polysaccharide, the yield of the crude extracellular polysaccharide EPS-OD is 131.31+ / -2.13 g / L after optimization, the extracellular polysaccharide EPS is obtained by purifying the crude EPS-OD through a DEAE ion exchange column, and the composition of the crude EPS-OD and the EPS is determined. The extracellular polysaccharide of the bacillus amyloliquefaciens D189 has good alpha-glucosidase inhibitory capacity and pancreatic lipase inhibitory capacity, and good total bile acid and cholesterol micelle binding capacity in vitro, so that the extracellular polysaccharide and the lipid-lowering and glucose-lowering product have good application prospects.

The invention relates to magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase based on amino acid ionic liquid modification as well as a preparation method and application of the magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase. The immobilized enzyme is prepared by taking Fe3O4 as a magnetic core, coating the magnetic core with dopamine, polymerizing to form a COF shell layer, modifying the surface of a carrier with amino acid ionic liquid (HisIL), and covalently immobilizing lipase (such as porcine pancreatic lipase), and the prepared immobilized enzyme is applied to catalytic synthesis of isoamyl acetate through esterification reaction.

This invention discloses a Chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity, its preparation method, and its applications. The amino acid sequence of this heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg. The preparation method includes: performing ultraviolet and electroporation combined mutagenesis on wild-type Chlorella proteoglycans to obtain a high-protein-content mutant algal strain; obtaining algal protein through fermentation and synergistic extraction and purification with alcohols; directionally digesting the protein with alkaline protease, followed by enriching the active peptide components through ultrafiltration and gel chromatography; and finally, screening the heptapeptide using liquid chromatography-mass spectrometry (LC-MS), computer-aided prediction, and molecular docking techniques. This heptapeptide exhibits potent inhibitory activity against pancreatic lipase and can dose-dependently reduce the accumulation of triglycerides and cholesterol in 3T3-L1 cells. Its mechanism involves multiple target pathways, including inhibition of MMP-9, activation of AMPK phosphorylation, and downregulation of PNLIP, and it can be used to develop lipid-lowering functional foods or health products.

The application belongs to the technical field of biological processing, and specifically discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide with lipid-lowering efficacy is composed of short peptides with a molecular weight less than 1000 Da; the short peptides are one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP. The application discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide has a significantly improved inhibition rate on cholesteryl esterase and pancreatic lipase, and has a stronger adsorption capacity on bile acid. The sea cucumber flower polypeptide does not contain the enzyme cutting sites of pepsin and trypsin, can resist secondary digestion and degradation after being taken into the human body, stably maintains structural integrity and biological activity, and solves the problem that traditional lipid-lowering peptides are easily destroyed in activity.

The invention relates to the technical field of plant extraction, in particular to a rose extract. The rose extract comprises at least one of monomer ellagic acid, urolithin M5 and apigenin. The preparation method comprises the following steps: uniformly mixing rose powder with the ternary synergistic solvent, carrying out ultrasonic treatment for 10-60 minutes under the ultrasonic power of 150-250 W, and then stirring for 1-5 hours at the temperature of 20-50 DEG C; centrifuging and collecting supernate to obtain the rose extract. The rose flower extract provided by the invention has relatively strong inhibition capability on human pancreatic lipase.

The invention belongs to the technical field of functional microorganism application, and particularly relates to a novel lipid-lowering peptide derived from a probiotic thallus and application thereof, the novel lipid-lowering peptide is a crushed substance derived from a probiotic lactobacillus plantarum thallus, and the molecular weight of the novel lipid-lowering peptide is less than 3kDa. And two key polypeptides APGFGDR (AP7) and TESGAFGR (TE8) are identified from the components, and can specifically inhibit the activities of pancreatic lipase and pancreatic cholesterol esterase. The extracted thallus peptide can be efficiently combined with cholate to inhibit the formation of cholesterol micelles, and has a remarkable inhibiting effect on pancreatic lipase and pancreatic cholesterol esterase. Animal experiments show that the mycopeptide can effectively improve lipid metabolism disorder induced by high fat diet, inhibit weight gain, reduce serum and liver lipid levels and relieve fatty degeneration and inflammation of the liver. The lipid-lowering peptide disclosed by the invention is high in safety and multi-target in action, and can be used for preparing functional products for preventing or treating metabolic diseases such as obesity, hyperlipidemia and the like.

The present application relates to the field of synthetic biology, and specifically discloses a preparation method for improving the biological activity of lentinan and application thereof. The method adopts white shiitake mushroom to perform solid-state fermentation on lentinan, and comprises the following steps: S1, grinding the lentinan into powder, then adding appropriate amount of water to stir uniformly, high-pressure sterilization, and cooling to obtain lentinan culture medium; S2, activating white shiitake mushroom strains and preparing white shiitake mushroom seed liquid, adding the white shiitake mushroom seed liquid into the lentinan culture medium according to a certain inoculation amount to perform solid-state fermentation; S3, taking the fermented lentinan culture medium, performing protease enzymolysis first, then adopting ethanol precipitation, centrifuging to take the precipitate, resolubilizing the precipitate, performing dialysis treatment through a dialysis bag, and freeze-drying the dialyzed solution to obtain lentinan. The preparation method significantly improves the yield and purity of lentinan, and at the same time, the antioxidant, anti-inflammatory and pancreatic lipase clearance abilities are significantly improved, and the method has the effect of lubricating intestines.

Described herein is a composition comprising at least one of a lotus leaf extract (LLE), a chrysanthemum extract (CME), and a Pu'er tea extract (PTE), the composition having an agonistic effect on the glucagon-like peptide-1 receptor (GLP-1R). In addition, described herein are compositions comprising a mixture of LLE, CME, and PTE that have a greater inhibitory effect on pancreatic lipase compared to the extract alone. Also described are methods of using the described compositions, e.g., for supporting weight loss efforts in a subject in need thereof.

The application discloses a milk-derived small molecular peptide, which has an amino acid sequence of Phe-Gly-Gly-Leu-Met (FGGLM) and a molecular weight of 523.64 Da. The small molecular peptide has the characteristics of inhibiting the activity of pancrelipases (PL) and reducing the accumulation of triglyceride (TG) content in oleic acid-induced human hepatoma (Hepg2) cells. The small molecular peptide can be applied to the preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, and has the characteristics of simple preparation, suitability for industrial production and market promotion and application.

The invention belongs to the technical field of microorganisms, and particularly provides a plant lactobacillus BUCM-1, the plant lactobacillus BUCM-1 is preserved in the China General Microbiological Culture Collection Center on June 16, 2025, the classification name of the plant lactobacillus BUCM-1 is plant lactobacillus, and the preservation number of the plant lactobacillus BUCM-1 is CGMCC NO. 34895. The plant lactobacillus BUCM-1 is named as plant lactobacillus. The lactobacillus plantarum BUCM-1 has relatively strong inhibitory ability to pancreatic lipase, relatively high total antioxidant ability and excellent cholesterol and triglyceride reducing ability, the survival rate is not greatly influenced after the lactobacillus plantarum BUCM-1 is cultured in artificial gastric juice for 3 hours, and the survival rate can be 65% after the lactobacillus plantarum BUCM-1 is continuously cultured in artificial intestinal juice for 8 hours. The invention further provides a preparation method of the plant lactobacillus fermented soybean juice, the process is simple, the quality is stable, and the prepared soybean juice has the effect of reducing blood fat.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

A method and device for determining pancreatic lipase in an animal sample is provided. In one aspect, the device includes a basic buffer layer containing one or more bile salts and calcium; a separator layer containing colipase; and an indicator layer containing a chromogenic substrate such as DGGR. The device may also include a system for optically determining the amount of color change that occurs. The method includes using the device to detect pancreatic lipase in an animal sample. In another aspect, the present disclosure provides a kit including the device described in various aspects and embodiments herein and instructions for use of the device.

A method of preparing a diterpenoid composition is provided. The method comprises the following steps: extracting a lysimachia capillipes material to obtain an extracted composition; and performing a series of separation steps to obtain at least one target extract of isolated diterpenoids of lysimachia capillipes. There is also provided a diterpenoid composition obtained by the method, an oral composition comprising the diterpenoid composition, a composition comprising at least one diterpenoid extract of lysimachia capillipes, the use of the composition in the preparation of a medicament for the treatment of hyperglycemia and / or hyperlipidemia in a subject, and the use of the composition in the treatment of hyperglycemia and / or hyperlipidemia in a subject. And methods of treating hyperglycemia and / or hyperlipidemia in a subject.