120 results about "Pancrelipase" patented technology

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to dendrobium officinale fermentation liquor with a pancreatic lipase inhibition effect and a preparation method of the dendrobium officinale fermentation liquor. The dendrobium officinale fermentation liquor is prepared by performing enzymolysis on dendrobium officinale and inoculating lactic acid bacteria for fermentation, wherein the dendrobium officinale fermentation liquor obtained by inoculating fermentation lactobacillus mucus DSM 13273 or leuconostoc mesenteroides subsp. Mesenteroides XD054 has a better pancreatic lipase activity inhibition effect.

The invention discloses a hemp seed protein source pancrelipase inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of bioactive peptides. The hemp seed protein source pancrelipase inhibitory peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO. 1 (YNLPILRF). The cannabis sativa L. protein source pancreatic lipase inhibitory peptide provided by the invention is separated from cannabis sativa L. protein for the first time, is a novel cannabis sativa L. protein source pancreatic lipase inhibitory peptide, has good pancreatic lipase inhibitory activity, can be used as a natural anti-obesity agent in food, health care products and weight control related drugs, and has broad application prospects. Good application prospects are realized.

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.

The invention discloses a horse milk-derived small molecule peptide, the amino acid sequence of which is Met-Ser-Pro-Phe (MSPF), and the molecular weight of which is 480.571 Da. The small molecule peptide disclosed by the invention has the activity of inhibiting pancreatic lipase (PL), the inhibition rate on the pancreatic lipase is 61.44%, and the accumulation of the content of triglyceride (TG) in oleic acid-induced human liver cancer (HepG2) cells can be reduced, so that the small molecule peptide can be used for inhibiting the pancreatic lipase (PL). The small molecule peptide can be applied to preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, is simple to prepare, and is suitable for industrial production and market popularization and application.

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention discloses a preparation method of a pilose antler mushroom extract and application of the pilose antler mushroom extract in products with nitrite adsorption and / or lipid lowering efficacy, SDF in pilose antler mushroom is extracted, it is found that the content of SDF in pilose antler mushroom is high, the pilose antler mushroom has good in-vitro lipid lowering activity, and the pilose antler mushroom is a high-quality edible mushroom raw material capable of being used for lipid lowering functional food development. A biological activity experiment shows that the pilose antler mushroom SDF extract can well remove DPPH and ABTS free radicals, adsorb nitrite and inhibit the activity of cholesterol esterase and pancreatic lipase, and shows good oxidation resistance, nitrite adsorption and lipid-lowering activity.

The invention relates to a lotus leaf polyphenol extract with a blood fat reducing effect as well as a preparation method and application of the lotus leaf polyphenol extract. The preparation method comprises the following steps that firstly, lotus leaf powder is pretreated through low-temperature plasmas, the treatment time is 400 W, the treatment power is 6 min, then a deep eutectic solvent serves as an extraction solvent, high-pressure auxiliary extraction is conducted on the pretreated lotus leaf powder, the ultrahigh pressure is 1.0 MPa, and the extraction time is 30 min; performing centrifugal filtration, concentration and drying to obtain a lotus leaf polyphenol extract; wherein the eutectic solvent is choline chloride-lactic acid-glycerol-water in a volume ratio of 1: 1: 1: 1. According to the method, the polyphenol compounds in the lotus leaves are extracted by combining the eutectic solvent with the plasma and high-pressure extraction technology, and compared with a traditional method, the method has the advantages of being simple, efficient, high in yield, free of organic reagent pollution, high in safety, easy to industrialize and the like. The lotus leaf polyphenol extract prepared by the invention has good pancreatic lipase inhibitory activity, which indicates that the lotus leaf polyphenol extract has the effect of reducing blood fat. The lotus leaf extract disclosed by the invention has great development value in the field of obesity, and also can promote high-value utilization of lotus leaf resources.

The invention discloses a method for screening lipid-lowering active substances from natural extracts by improving enzyme immobilization efficiency, compared with the prior art, a polypropylene hollow fiber membrane is used as an adsorption carrier, glutaraldehyde is used as a cross-linking agent, pancreatic lipase is covalently cross-linked to the polypropylene hollow fiber membrane, and the lipid-lowering active substances are screened from the natural extracts. The enzyme loading amount, the repeated utilization rate and the storage stability of the polypropylene hollow fiber membrane are obviously improved; the enzyme immobilization efficiency and the enzyme activity are improved; furthermore, liquid chromatography-quadrupole time-of-flight mass spectrometry and a molecular network analysis technology are combined to screen 31 active ligand structures from the wild chrysanthemum flower extract with numerous and jumbled components, so that the method can be popularized and used for efficiently screening active ligands in natural products with complex components; the material basis of the lipid-lowering effect of the natural-source active ingredients can be deeply illuminated, and the active ligand has certain lipid-lowering potential and can be used as a pilot chemical of lipid-lowering drugs.

The invention relates to the field of microbial fermentation food, and discloses application of lactobacillus plantarum, a prune fermentation product, a preparation method of the prune fermentation product and a prune fermentation strain. A strain of lactobacillus plantarum with better performance is obtained from the nature, the preservation number is CCTCC NO: M 20242280, and after the lactobacillus plantarum is used for fermenting the prune subjected to enzymolysis, compared with other fermentation strains and fermentation products without fermentation strains, the obtained fermentation product has the advantages that the fermentation yield is increased, and the yield is increased. The pancreatic lipase inhibition rate and the cholesterol micelle solubility inhibition rate are remarkably improved, and the compound shows extremely excellent activity of improving blood fat. The invention not only provides an effective, time-saving and energy-saving preparation process method of the prune fermented product, but also provides a wide foundation for further application of the prune.

The application relates to the technical field of microbial fermentation, and discloses a lily fermentation product for improving blood lipid, a preparation method and application thereof. Lily raw materials are taken as fermentation objects, and suitable lactobacillus plantarum fermentation strains are selected to ferment the lily raw materials to obtain a lily fermentation product. Compared with other fermentation products of lactobacillus plantarum, the lily fermentation product of the lactobacillus plantarum of CICC 25125 has more excellent pancreatic lipase inhibition capacity and has the effect of promoting and improving blood lipid levels, thereby providing a better basis for the application of the lily fermentation product in the food field.

The application discloses a small-molecule peptide from fermented mare milk, which has an amino acid sequence of His-Ala-Trp-Phe and a molecular weight of 559.67 Da. The small-molecule peptide has pancreatic lipase inhibitory activity, can be applied to the preparation of a medicine for reducing high blood fat, and is simple to prepare, suitable for industrialized production and market promotion and application.

The utility model relates to the technical field of lipase detection devices, and discloses a cat pancreas lipase detection device which comprises a kit, a placing frame is fixed in the kit, and three groups of first placing grooves and second placing grooves are sequentially formed in the top surface of the placing frame from front to back in the width direction; each group comprises a first placing groove and three second placing grooves, and the fixing assembly comprises a fixed clamping plate arranged in the first placing groove and a movable clamping plate arranged in the second placing groove. According to the kit, the three groups of detection reagents and detection tools are respectively clamped and fixed by adopting the three groups of fixed clamping plates and movable clamping plates, and when one group of detection reagents and detection tools is unfastened, the detection reagents and detection tools of other groups are still in a clamped and fixed state, so that the stability of the reagents during transportation of the kit is improved; meanwhile, the situation that when a group of cat pancreatic lipase blood samples are detected, a worker mistakenly takes reagents and detection tools of other test groups, and detection work is affected is prevented.

The invention discloses a method for extracting tetrandrine in stephania tetrandra, which comprises the following steps: (1) mixing stephania tetrandra and ethanol, extracting, collecting filtrate, and removing ethanol to obtain stephania tetrandra extract; (2) mixing organic matters, inorganic salts and water to obtain an aqueous two-phase system; and (3) adding the stephania tetrandra extract into the aqueous two-phase system, and then carrying out ultrasonic treatment, shaking extraction and centrifugation to obtain an upper-phase system and a lower-phase system which contain tetrandrine. According to the method for extracting the tetrandrine in the stephania tetrandra, an aqueous two-phase extraction method and an ultrasonic technology are combined, the extraction rate of the tetrandrine in the stephania tetrandra is taken as a target, and an extraction and separation process of the tetrandrine in the stephania tetrandra extract is optimized by adopting the aqueous two-phase extraction method. The tetrandrine extracted by the ultrasonic-assisted aqueous two-phase method is high in yield and low in solvent residue. The extract obtained by the method has a good inhibition effect on pancreatic lipase.

The invention relates to a calf bone marrow hydrolysate liposome freeze-dried powder and its application in hematopoiesis-promoting and anti-aging products, belonging to the field of biotechnology. The calf bone marrow hydrolysate liposome freeze-dried powder is a product obtained by using calf bone marrow as a raw material, and enzymolyzing the product step by step with pancreatic lipase, alkaline phosphatase, trypsin, elastase, and ficin to obtain an enzymolyzate below 3 kDa, and then coating the product with liposomes. The product is rich in multiple small and medium molecule nutritional active substances, growth factors, and small molecule peptide components. The combination of each component can enhance the efficacy, can more effectively promote hematopoiesis, and also has excellent antioxidant ability, removes free radicals in the body, reduces the damage of oxidation reactions to cells and tissues, and effectively delays the aging process.

The invention discloses a small molecule peptide which is derived from fermented horse milk, the amino acid sequence of the small molecule peptide is Phe-Ser-Pro-Phe, and the molecular weight of the small molecule peptide is 496.549 Da. The small molecule peptide disclosed by the invention has the activity of inhibiting pancreatic lipase (PL), the highest inhibition rate of the small molecule peptide to the pancreatic lipase is 58.58%, the accumulation of the content of triglyceride (TG) in oleic acid induced human liver cancer (HepG2) cells can be reduced, and the small molecule peptide can be used for preparing a medicine for treating liver cancer. The small molecule peptide disclosed by the invention can be applied to preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, and has the characteristics of simplicity in preparation and suitability for industrial production and market popularization and application.

The invention discloses a separation process of a pancreatic lipase inhibitor Lycrutheophylpropanoid J with a new structure in red Chinese wolfberry fruits and application of the pancreatic lipase inhibitor Lycrutheophylpropanoid J with the new structure in the red Chinese wolfberry fruits. The specific preparation process comprises the following seven steps: extraction, on-line pancreatic lipase inhibitor component recognition, hydrophilic water pressure preparation column coarse separation, reversed-phase high-pressure preparation column preparation, hydrophilic high-pressure preparation column preparation, gel filtration chromatography and in-vitro pancreatic lipase inhibition experiment. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by the invention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch material preparation is easy; in the preparation process, solvents and separation materials used by an extraction solvent, a hydrophilic preparative column, a reversed-phase preparative column, gel chromatography and a reversed-phase chromatographic column can be recycled.

The invention discloses a separation process of a pancreatic lipase inhibitor Lycrutheophylpropanoid H with a new structure in red Chinese wolfberry fruits and application of the pancreatic lipase inhibitor Lycrutheophylpropanoid H with the new structure in the red Chinese wolfberry fruits. The specific preparation process comprises the following seven steps: extraction, on-line pancreatic lipase inhibitor component recognition, hydrophilic water pressure preparation column coarse separation, reversed-phase high-pressure preparation column preparation, hydrophilic high-pressure preparation column preparation, gel filtration chromatography and in-vitro pancreatic lipase inhibition experiment. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by the invention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch material preparation is easy; in the preparation process, solvents and separation materials used by an extraction solvent, a hydrophilic preparative column, a reversed-phase preparative column, gel chromatography and a reversed-phase chromatographic column can be recycled.

The invention provides a fermentation product of seedless clausena lansium as well as a preparation method and application of the fermentation product, and the fermentation product is prepared by performing ultrasonic-assisted fermentation on seedless clausena lansium through fermentation lactobacillus mucus Q7 with the preservation number of GDMCC NO: 65149. The preparation method comprises the following steps: pretreating a seedless clausena lansium raw material, inoculating the seedless clausena lansium raw material with an activated bacterial solution of the strain, and fermenting under the optimal process conditions that the fermentation time is 52 hours, the inoculation amount is 7%, the fermentation temperature is 37 DEG C, the ultrasonic power is 100 W and the ultrasonic time is 63 minutes. According to the method, the active ingredients of the product are remarkably improved, the total phenol content reaches 4.70 mg GAE / g, and the pancreatic lipase inhibition rate reaches 96.30%. The fermentation product is rich in phenols, flavones and organic acids, has excellent antioxidant activity and lipid-lowering activity, and can be used for developing functional food, health care products or medicines.

The application discloses a mulberry leaf astringency removing treatment process and super-micro powder thereof. The process mainly uses a fermented coix as an astringency removing agent to remove the astringency of frost mulberry leaves in combination with three steaming and hot air drying methods. The application solves the problems of astringency and green smell of the mulberry leaves by using a relatively simple process, retains the unique taste and fragrance of the mulberry leaves, and adds unique alcohol and ester fragrance, so that the taste and smell of the mulberry leaves are rich in layers. The prepared astringency removed mulberry leaf super-micro powder has high retention rate of main nutritional components, has obvious inhibition effect on alpha-glucosidase and pancreatic lipase, and can be used as a food base to develop health food for reducing blood sugar and blood fat.

The invention relates to the technical field of functional food, in particular to a tartary buckwheat and roxburgh rose composition for regulating blood pressure and blood fat and a preparation method thereof. The tartary buckwheat and roxburgh rose composition comprises tartary buckwheat and roxburgh rose. The mass ratio of the tartary buckwheat to the roxburgh rose is 1: (1-3). In-vitro activity evaluation, cell experiments and animal experiments prove that flavonoid compounds of tartary buckwheat and active ingredients such as vitamin C of roxburgh rose in the composition generate a synergistic effect, so that the antioxidant capacity, the ACE inhibition rate, the cholesterol clearance rate and the pancreatic lipase inhibition rate can be remarkably improved, NO generation is effectively promoted, and the curative effect is good. The contents of TC, TG and LDL-L are reduced, the HDL-L level is increased, and the balance of vascular active substances is regulated. The raw materials are all medicinal and edible, are high in safety and free of side effects, provide a natural and efficient auxiliary conditioning scheme for chronic metabolic diseases such as hypertension and hyperlipidemia, and have a good industrialization prospect.

A novel polypeptide with double biological activities is obtained from sea cucumber intestines, and the sequence of the polypeptide is KADANNQWVDVK through high performance liquid chromatography purification and mass spectrometry analysis and identification. The in-vitro activity evaluation of a system shows that the polypeptide not only shows remarkable antioxidant capacity and can effectively remove ABTS free radicals, but also shows relatively strong pancreatic lipase inhibitory activity, and the IC50 value of the polypeptide reaches a remarkable level. The marine active peptide with double functions of oxidation resistance and metabolism regulation shows a good application prospect in the field of functional food and medicine development. The sea cucumber intestines are treated through an enzymolysis technology, low-molecular-weight polypeptides are separated through dialysis, de novo sequencing is carried out, and high-throughput polypeptides are synthesized. The polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is screened, and the polypeptide KADANNQWVDVK (lysine-alanine-aspartic acid-alanine-asparagine-asparagine-asparagine-glutamine-tryptophan-valine-asparagine-valine-lysine) with the activity of resisting oxidation and inhibiting pancreatic lipase is verified. According to the method, sea cucumber processing byproducts are fully utilized, the resource utilization rate is increased, a scientific basis is provided for high-value utilization of sea cucumber intestines, and meanwhile, a potential material basis is provided for developing natural antioxidants and peptides with lipid-lowering functions.

The invention discloses a small molecule peptide derived from fermented horse milk, the amino acid sequence of the small molecule peptide is His-Ala-Trp-Phe, and the molecular weight of the small molecule peptide is 559.67 Da. The small molecule peptide has pancreatic lipase inhibitory activity, can be applied to preparation of special medical foods and medicines for reducing hyperlipidemia, and is simple to prepare and suitable for industrial production and market popularization and application.

This invention discloses a method for screening pancreatic lipase inhibitory peptides based on machine learning, relating to the fields of bioinformatics and food science. The method includes the following steps: dataset construction and three-dimensional peptide structure characterization, statistical-based feature screening, outlier removal based on a dual-model consensus mechanism, feature recursive elimination and physicochemical significance optimization, data augmentation based on Gaussian noise injection, and final prediction model construction. This invention effectively solves the problems of insufficient feature representation, high noise interference, and weak model generalization ability in existing screening methods through multi-dimensional feature characterization, rigorous outlier cleaning, and data augmentation strategies for small samples. It can quickly and accurately screen highly active pancreatic lipase inhibitory peptides from massive peptide sequences, providing an efficient computational tool for the development of functional foods and lipid-lowering drugs.

The invention belongs to the technical field of biology, and relates to a preparation method and application of a shark nano antibody targeting pancreatic lipase. The amino acid sequence of the shark nano antibody VNAR-A4 is as shown in SEQ ID No. 10. The molecular weight of the shark nano antibody VNAR-A4 prepared by the invention is about 15 kDa, is smaller than that of a traditional monoclonal antibody, has a slender CDR3 structure, and can be better combined with hidden epitopes of antigens; the shark nano antibody VNAR-A4 is good in stability, can keep activity under an extreme pH condition, and has good affinity and specificity with PL; the shark nano antibody VNAR-A4 is expressed by using an escherichia coli prokaryotic expression system, is easier for genetic engineering modification, and can be prepared in a large scale. The shark nano antibody VNAR-A4 has the potential of inhibiting the activity of pancreatic lipase, and a novel fat hydrolysis inhibiting reagent is provided for treating obesity.

The application belongs to the technical field of microorganisms, and discloses a bacillus amyloliquefaciens D189 with high yield of extracellular polysaccharide. The bacillus amyloliquefaciens D189 is preserved in China Center for Type Culture Collection on April 29, 2021, and the preservation number is CCTCC NO: M2021484. The bacillus amyloliquefaciens D189 provided in the application has high yield of extracellular polysaccharide, the yield of the crude extracellular polysaccharide EPS-OD is 131.31+ / -2.13 g / L after optimization, the extracellular polysaccharide EPS is obtained by purifying the crude EPS-OD through a DEAE ion exchange column, and the composition of the crude EPS-OD and the EPS is determined. The extracellular polysaccharide of the bacillus amyloliquefaciens D189 has good alpha-glucosidase inhibitory capacity and pancreatic lipase inhibitory capacity, and good total bile acid and cholesterol micelle binding capacity in vitro, so that the extracellular polysaccharide and the lipid-lowering and glucose-lowering product have good application prospects.

The invention relates to magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase based on amino acid ionic liquid modification as well as a preparation method and application of the magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase. The immobilized enzyme is prepared by taking Fe3O4 as a magnetic core, coating the magnetic core with dopamine, polymerizing to form a COF shell layer, modifying the surface of a carrier with amino acid ionic liquid (HisIL), and covalently immobilizing lipase (such as porcine pancreatic lipase), and the prepared immobilized enzyme is applied to catalytic synthesis of isoamyl acetate through esterification reaction.