39 results about "Pancrelipase" patented technology

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The application relates to the technical field of microbial fermentation, and discloses a lily fermentation product for improving blood lipid, a preparation method and application thereof. Lily raw materials are taken as fermentation objects, and suitable lactobacillus plantarum fermentation strains are selected to ferment the lily raw materials to obtain a lily fermentation product. Compared with other fermentation products of lactobacillus plantarum, the lily fermentation product of the lactobacillus plantarum of CICC 25125 has more excellent pancreatic lipase inhibition capacity and has the effect of promoting and improving blood lipid levels, thereby providing a better basis for the application of the lily fermentation product in the food field.

The application discloses a mulberry leaf astringency removing treatment process and super-micro powder thereof. The process mainly uses a fermented coix as an astringency removing agent to remove the astringency of frost mulberry leaves in combination with three steaming and hot air drying methods. The application solves the problems of astringency and green smell of the mulberry leaves by using a relatively simple process, retains the unique taste and fragrance of the mulberry leaves, and adds unique alcohol and ester fragrance, so that the taste and smell of the mulberry leaves are rich in layers. The prepared astringency removed mulberry leaf super-micro powder has high retention rate of main nutritional components, has obvious inhibition effect on alpha-glucosidase and pancreatic lipase, and can be used as a food base to develop health food for reducing blood sugar and blood fat.

The invention relates to the technical field of functional food, in particular to a tartary buckwheat and roxburgh rose composition for regulating blood pressure and blood fat and a preparation method thereof. The tartary buckwheat and roxburgh rose composition comprises tartary buckwheat and roxburgh rose. The mass ratio of the tartary buckwheat to the roxburgh rose is 1: (1-3). In-vitro activity evaluation, cell experiments and animal experiments prove that flavonoid compounds of tartary buckwheat and active ingredients such as vitamin C of roxburgh rose in the composition generate a synergistic effect, so that the antioxidant capacity, the ACE inhibition rate, the cholesterol clearance rate and the pancreatic lipase inhibition rate can be remarkably improved, NO generation is effectively promoted, and the curative effect is good. The contents of TC, TG and LDL-L are reduced, the HDL-L level is increased, and the balance of vascular active substances is regulated. The raw materials are all medicinal and edible, are high in safety and free of side effects, provide a natural and efficient auxiliary conditioning scheme for chronic metabolic diseases such as hypertension and hyperlipidemia, and have a good industrialization prospect.

This invention discloses a method for screening pancreatic lipase inhibitory peptides based on machine learning, relating to the fields of bioinformatics and food science. The method includes the following steps: dataset construction and three-dimensional peptide structure characterization, statistical-based feature screening, outlier removal based on a dual-model consensus mechanism, feature recursive elimination and physicochemical significance optimization, data augmentation based on Gaussian noise injection, and final prediction model construction. This invention effectively solves the problems of insufficient feature representation, high noise interference, and weak model generalization ability in existing screening methods through multi-dimensional feature characterization, rigorous outlier cleaning, and data augmentation strategies for small samples. It can quickly and accurately screen highly active pancreatic lipase inhibitory peptides from massive peptide sequences, providing an efficient computational tool for the development of functional foods and lipid-lowering drugs.

The application belongs to the technical field of microorganisms, and discloses a bacillus amyloliquefaciens D189 with high yield of extracellular polysaccharide. The bacillus amyloliquefaciens D189 is preserved in China Center for Type Culture Collection on April 29, 2021, and the preservation number is CCTCC NO: M2021484. The bacillus amyloliquefaciens D189 provided in the application has high yield of extracellular polysaccharide, the yield of the crude extracellular polysaccharide EPS-OD is 131.31+ / -2.13 g / L after optimization, the extracellular polysaccharide EPS is obtained by purifying the crude EPS-OD through a DEAE ion exchange column, and the composition of the crude EPS-OD and the EPS is determined. The extracellular polysaccharide of the bacillus amyloliquefaciens D189 has good alpha-glucosidase inhibitory capacity and pancreatic lipase inhibitory capacity, and good total bile acid and cholesterol micelle binding capacity in vitro, so that the extracellular polysaccharide and the lipid-lowering and glucose-lowering product have good application prospects.

The invention relates to magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase based on amino acid ionic liquid modification as well as a preparation method and application of the magnetic covalent organic framework (COF) composite nanomaterial immobilized lipase. The immobilized enzyme is prepared by taking Fe3O4 as a magnetic core, coating the magnetic core with dopamine, polymerizing to form a COF shell layer, modifying the surface of a carrier with amino acid ionic liquid (HisIL), and covalently immobilizing lipase (such as porcine pancreatic lipase), and the prepared immobilized enzyme is applied to catalytic synthesis of isoamyl acetate through esterification reaction.

This invention discloses a Chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity, its preparation method, and its applications. The amino acid sequence of this heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg. The preparation method includes: performing ultraviolet and electroporation combined mutagenesis on wild-type Chlorella proteoglycans to obtain a high-protein-content mutant algal strain; obtaining algal protein through fermentation and synergistic extraction and purification with alcohols; directionally digesting the protein with alkaline protease, followed by enriching the active peptide components through ultrafiltration and gel chromatography; and finally, screening the heptapeptide using liquid chromatography-mass spectrometry (LC-MS), computer-aided prediction, and molecular docking techniques. This heptapeptide exhibits potent inhibitory activity against pancreatic lipase and can dose-dependently reduce the accumulation of triglycerides and cholesterol in 3T3-L1 cells. Its mechanism involves multiple target pathways, including inhibition of MMP-9, activation of AMPK phosphorylation, and downregulation of PNLIP, and it can be used to develop lipid-lowering functional foods or health products.

The application belongs to the technical field of biological processing, and specifically discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide with lipid-lowering efficacy is composed of short peptides with a molecular weight less than 1000 Da; the short peptides are one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP. The application discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide has a significantly improved inhibition rate on cholesteryl esterase and pancreatic lipase, and has a stronger adsorption capacity on bile acid. The sea cucumber flower polypeptide does not contain the enzyme cutting sites of pepsin and trypsin, can resist secondary digestion and degradation after being taken into the human body, stably maintains structural integrity and biological activity, and solves the problem that traditional lipid-lowering peptides are easily destroyed in activity.

The invention relates to the technical field of plant extraction, in particular to a rose extract. The rose extract comprises at least one of monomer ellagic acid, urolithin M5 and apigenin. The preparation method comprises the following steps: uniformly mixing rose powder with the ternary synergistic solvent, carrying out ultrasonic treatment for 10-60 minutes under the ultrasonic power of 150-250 W, and then stirring for 1-5 hours at the temperature of 20-50 DEG C; centrifuging and collecting supernate to obtain the rose extract. The rose flower extract provided by the invention has relatively strong inhibition capability on human pancreatic lipase.

The invention belongs to the technical field of functional microorganism application, and particularly relates to a novel lipid-lowering peptide derived from a probiotic thallus and application thereof, the novel lipid-lowering peptide is a crushed substance derived from a probiotic lactobacillus plantarum thallus, and the molecular weight of the novel lipid-lowering peptide is less than 3kDa. And two key polypeptides APGFGDR (AP7) and TESGAFGR (TE8) are identified from the components, and can specifically inhibit the activities of pancreatic lipase and pancreatic cholesterol esterase. The extracted thallus peptide can be efficiently combined with cholate to inhibit the formation of cholesterol micelles, and has a remarkable inhibiting effect on pancreatic lipase and pancreatic cholesterol esterase. Animal experiments show that the mycopeptide can effectively improve lipid metabolism disorder induced by high fat diet, inhibit weight gain, reduce serum and liver lipid levels and relieve fatty degeneration and inflammation of the liver. The lipid-lowering peptide disclosed by the invention is high in safety and multi-target in action, and can be used for preparing functional products for preventing or treating metabolic diseases such as obesity, hyperlipidemia and the like.

Described herein is a composition comprising at least one of a lotus leaf extract (LLE), a chrysanthemum extract (CME), and a Pu'er tea extract (PTE), the composition having an agonistic effect on the glucagon-like peptide-1 receptor (GLP-1R). In addition, described herein are compositions comprising a mixture of LLE, CME, and PTE that have a greater inhibitory effect on pancreatic lipase compared to the extract alone. Also described are methods of using the described compositions, e.g., for supporting weight loss efforts in a subject in need thereof.

The invention belongs to the technical field of microorganisms, and particularly provides a plant lactobacillus BUCM-1, the plant lactobacillus BUCM-1 is preserved in the China General Microbiological Culture Collection Center on June 16, 2025, the classification name of the plant lactobacillus BUCM-1 is plant lactobacillus, and the preservation number of the plant lactobacillus BUCM-1 is CGMCC NO. 34895. The plant lactobacillus BUCM-1 is named as plant lactobacillus. The lactobacillus plantarum BUCM-1 has relatively strong inhibitory ability to pancreatic lipase, relatively high total antioxidant ability and excellent cholesterol and triglyceride reducing ability, the survival rate is not greatly influenced after the lactobacillus plantarum BUCM-1 is cultured in artificial gastric juice for 3 hours, and the survival rate can be 65% after the lactobacillus plantarum BUCM-1 is continuously cultured in artificial intestinal juice for 8 hours. The invention further provides a preparation method of the plant lactobacillus fermented soybean juice, the process is simple, the quality is stable, and the prepared soybean juice has the effect of reducing blood fat.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

A method of preparing a diterpenoid composition is provided. The method comprises the following steps: extracting a lysimachia capillipes material to obtain an extracted composition; and performing a series of separation steps to obtain at least one target extract of isolated diterpenoids of lysimachia capillipes. There is also provided a diterpenoid composition obtained by the method, an oral composition comprising the diterpenoid composition, a composition comprising at least one diterpenoid extract of lysimachia capillipes, the use of the composition in the preparation of a medicament for the treatment of hyperglycemia and / or hyperlipidemia in a subject, and the use of the composition in the treatment of hyperglycemia and / or hyperlipidemia in a subject. And methods of treating hyperglycemia and / or hyperlipidemia in a subject.

The invention belongs to the technical field of pet food processing, and particularly relates to a staple food soup pot for kittens and a preparation method thereof.The staple food soup pot for kittens comprises a fiberized meat base and a soup base; the fiberized meat base is in a sheet shape or a block shape, and the fiberized meat base has a three-dimensional cross-linked network structure; the soup base contains nutritional supplements. Through synergistic pretreatment of pancreatic lipase and flavourzyme, macromolecular protein and fat are decomposed into small molecular peptide, amino acid and free fatty acid which are more easily absorbed by intestinal tracts of kittens, and the nutrition titer and bioavailability of the product are remarkably improved.

The invention belongs to the technical field of bioactive peptides, and provides a wheat peptide with lipid-lowering activity, application of the wheat peptide and a wheat oligopeptide noodle product, and the amino acid sequence of the wheat peptide is QLPPF, GPPFPQ, NGAGPPF or QPYPFPQ. The wheat peptide disclosed by the invention is prepared by performing enzymolysis on common foods by using a food enzyme, and meets the food raw material standard; the pancreatic lipase inhibitor has the characteristics of naturalness, no toxicity, no carcinogenicity, small molecular weight, no digestion by gastrointestinal tracts and high pancreatic lipase inhibition activity, is simple in preparation method and easy for industrial production, and can be prepared on a large scale and applied to foods such as noodles, health care products and medicines.

The application provides a method for detecting the content of Sn-2 DHA in DHA formulated oil, comprising: pretreating a sample to be detected; hydrolyzing and separating the pretreated sample to obtain DHA oil; and detecting the content of Sn-2 DHA in the DHA oil; wherein the hydrolyzing comprises: hydrolyzing the pretreated sample by using pancrelipase; the pancrelipase is used in an amount of at least 2000 mg per 1 g of the pretreated sample, and the hydrolysis is performed for at least 10 minutes. The application provides a method for detecting the content of Sn-2 DHA, which can effectively improve the accuracy of detecting the content of Sn-2 DHA in DHA oil, especially the accuracy of detecting the content of Sn-2 DHA in DHA formulated oil, by improving the hydrolysis degree of the hydrolysis process, which is of great significance for quality control of DHA oil products and fatty acid analysis.

This invention relates to the pharmaceutical field, specifically to an egg yolk-derived peptide for lowering blood lipids, its preparation method, pharmaceutical composition, and uses. This invention discovers an egg yolk-derived peptide obtained by enzymatic hydrolysis, digestion, separation, and purification of defatted egg yolk powder. Its amino acid sequence is shown in SEQ ID NO.1. This peptide possesses high potential activity for lowering blood lipids. In vitro pancreatic lipase inhibition experiments and hyperlipidemic cell model experiments demonstrate that the egg yolk-derived peptide of this invention can affect lipid absorption and metabolism, thereby alleviating lipid metabolism disorders and lipid accumulation, ultimately reducing TC and TG levels.

This invention belongs to the field of comprehensive utilization of food by-products and condiment processing technology, specifically relating to a method for preparing a seasoning base material of rice wine lees based on the directional control of the Maillard reaction through hot air drying and its application. Pretreated rice wine lees are hot-air dried at 100-140℃, with controlled temperature and time. Lees treated at 100℃ exhibit reduced bitterness and optimal umami, salty-savory balance, and overall acceptability, making them suitable as a high-quality auxiliary ingredient in compound seasonings such as chicken essence. Lees treated at 140℃ show increased ABTS free radical scavenging rate and inhibitory activity against pancreatic lipase and cholesterol esterase, possessing both antioxidant and lipid-lowering functions. This invention achieves directional control of the Maillard reaction solely through precise temperature control of hot air drying, eliminating the need for added exogenous amino acids or sugars. This transforms rice wine lees, a low-value by-product, into a seasoning base material with characteristic flavor and functional activity, significantly increasing added value.

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses application of a traditional Chinese medicine extract in preparation of a lipid-lowering product. The traditional Chinese medicine extract is prepared from broadleaf holly leaves, lotus leaves, licorice roots and Chinese dates. The preparation method comprises the following steps: mixing the broadleaf holly leaves, the lotus leaves, the liquorice and the Chinese dates, and extracting with water; after the extraction is finished, taking an extracting solution, concentrating and drying to obtain a water extract, and taking the water extract to obtain the traditional Chinese medicine extract. Researches show that the traditional Chinese medicine extract disclosed by the invention has an inhibition effect on cholesterol esterase and pancreatic lipase; therefore, when being used as an active part for preparing a lipid-lowering product for treating and / or preventing hyperlipidemia, the compound has an important application value.

The application belongs to the technical field of natural medicinal chemistry and enzyme inhibitor development, and particularly relates to a preparation method and application of an active ingredient of red bean. The active ingredient is (+) catechin 7-O-beta-D-glucopyranoside accurately separated from red bean. In vitro experiments prove that the active ingredient has strong inhibitory effect on alpha-glucosidase and pancreatic lipase. Cell and animal experiments further prove that the active ingredient can significantly inhibit the differentiation and lipid accumulation of 3T3-L1 preadipocytes, and can effectively reduce the body weight, body fat and blood lipid level of high-fat diet induced obese mice, and improve liver steatosis. The application plays a multi-target anti-obesity effect by synergistically inhibiting the digestion and absorption of sugar and fat, and has a wide application prospect in the preparation of medicines and functional foods for preventing and treating obesity, type II diabetes and other metabolic diseases.

The invention discloses a chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity as well as a preparation method and application of the chlorella heptapeptide TGPLGSR. The amino acid sequence of the heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg, and the amino acid sequence of the heptapeptide is as shown in the specification. The preparation method comprises the following steps: performing ultraviolet and electric shock compound mutagenesis on wild chlorella pyrenoidosa to obtain a mutagenic algal strain with high protein content; carrying out fermentation and alcoholase synergistic extraction and purification to obtain algae protein; after protein is subjected to directional enzymolysis by adopting alkaline protease, active peptide components are enriched through ultrafiltration and gel chromatography in sequence; finally, the heptapeptide is obtained through liquid chromatography-mass spectrometry identification, computer-aided prediction and molecular docking technology screening. The heptapeptide has strong inhibitory activity on pancreatic lipase and can reduce accumulation of triglyceride and cholesterol in 3T3-L1 cells in a dose-dependent manner, the mechanism of the heptapeptide relates to multi-target pathways such as MMP-9 inhibition, AMPK phosphorylation activation and PNLIP down-regulation, and the heptapeptide can be used for developing lipid-lowering functional foods or health-care products.

The invention relates to the technical field of microorganisms, in particular to lactobacillus acidophilus Deepsea100 with functions of reducing blood sugar, reducing fat and resisting aging and application of the lactobacillus acidophilus Deepsea100. The lactobacillus acidophilus Deepsea100 is separated from the intestinal tract of New Zealand longevity fish, and the preservation number is CGMCC No.36868. The scavenging rate of a fermented product to DPPH free radicals is larger than or equal to 96%, the scavenging rate to hydroxyl free radicals is larger than or equal to 83%, the inhibition rates to alpha-amylase, alpha-glucosidase and pancreatic lipase are larger than or equal to 71%, larger than or equal to 65% and larger than or equal to 66% respectively, and the lactobacillus acidophilus Deepsea100 has the effects of resisting oxidation, reducing blood sugar and fat and resisting aging, and can be used for preparing a feed additive. And the feed is strong in gastric acid intestinal juice resistance and intestinal colonization capacity, free of hemolytic reaction, sensitive to antibiotics and high in food safety. The invention also provides a microbial agent, a probiotic product, a fermentation product and the like containing the strain, can be applied to food, health care products, cosmetics or animal feeds, has the functions of prolonging the service life, enhancing the reproductive capacity and the like, and is remarkable in application value.

Disclosed in the present invention is a use of Lacticaseibacillus paracasei and a fermented product thereof for enhancing digestive enzyme activity. The Lacticaseibacillus paracasei is Lacticaseibacillus paracasei PC-01 with the preservation number of CGMCC No. 17537. In the present invention, the Lacticaseibacillus paracasei PC-01 can significantly enhance the activities of lactase, sucrase, and aminopeptidase in a small intestine, and the activity of trypsin in a pancreas; and the fermented product of PC-01 can further significantly enhance the activity of pancreatic lipase in the pancreas, and enhancement of the activities of lactase, aminopeptidase, and pancreatic lipase by the fermented product of PC-01 is significantly superior to that by PC-01.

The invention discloses a modification technology for soybean soluble dietary fibers based on UHP. The modification technology comprises the following steps: pretreating SDF raw materials; extracting soluble dietary fibers from the bean dregs; determining basic physicochemical properties, and determining monosaccharide composition; determining the WHC and the OHC of the SDF; analysis is carried out through a scanning electron microscope (SEM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD); soluble dietary fibers of bean dregs are modified by adopting a UHP technology, and a result shows that WHC, OHC and SC of the bean dreg dietary fibers, the antioxidant capacity, the glucose adsorption capacity, the alpha-amylase inhibition capacity and the alpha-glucosidase inhibition capacity (p < lt >; 0.05), cholesterol adsorption capacity, in-vitro bile salt binding capacity and pancreatic lipase inhibition capacity, which indicate that the soybean dreg soluble dietary fiber adsorption capacity, in-vitro blood sugar reducing activity and in-vitro blood fat reducing property are better.

This invention discloses a method and application for extracting soluble dietary fiber from soybean residue using a pulsed electric field assisted extraction. The method includes dissolving dried, pulverized, and sieved soybean residue powder in distilled water to obtain a soybean residue liquid; subjecting the soybean residue liquid to repeated freeze-thaw cycles followed by pulsed electric field treatment; adding a compound enzyme to the pulsed electric field-treated soybean residue liquid for enzymatic hydrolysis; after enzymatic hydrolysis, inactivating the enzyme, centrifuging to collect the supernatant, and then concentrating, precipitating with alcohol, and freeze-drying the supernatant to obtain soluble dietary fiber from soybean residue. This invention employs a synergistic extraction process of repeated freeze-thaw cycles, followed by a pulsed electric field, and then compound enzymatic hydrolysis, which can effectively improve the extraction rate of soluble dietary fiber from soybean residue. The obtained soluble dietary fiber from soybean residue exhibits excellent antioxidant activity and pancreatic lipase inhibitory activity, while its physicochemical properties are significantly improved, making it suitable for use in functional foods and food additives, with outstanding industrial application value.

The invention provides a method for biologically modifying kelp fucoidan by utilizing monascus fermentation, a product and application thereof. The method comprises the step of fermenting kelp by utilizing monascus. According to the method, monascus fermentation is adopted, so that the total sugar content of fucoidan can be remarkably increased, and the content of sulfate groups and uronic acid is reduced to a certain extent; compared with an unfermented kelp product, the cholesterol binding rate, the cholate adsorption capacity and the pancreatic lipase inhibition rate of the kelp fucoidan prepared by the method disclosed by the invention are improved, so that the kelp fucoidan prepared by the method disclosed by the invention has better blood fat reducing activity; in addition, compared with some existing hypolipidemic products, the kelp fucoidan prepared by the invention has higher cholesterol binding capacity.

The application discloses a small molecule peptide, which has an amino acid sequence of Ser-Pro-Cys-Pro-Met (SPCPM) and a molecular weight of 533.64 Da. The small molecule peptide has the advantages of inhibiting the activity of pancreatic lipase and the inhibition rate of pancreatic lipase is 63.42%. The novel small molecule peptide can reduce the accumulation of triglyceride (TG) content in oleic acid-induced human hepatoma cells (Hepg2) (human hepatoma) and reduce the content of lipid droplets in oleic acid-induced Hepg2 cells. The small molecule peptide can be applied to the preparation of a medicine for treating or assisting in treating obesity, and has the advantages of simple preparation, suitability for industrial production and market promotion and application.

The invention relates to preparation and application of honeysuckle fermentation liquor for assisting in controlling body weight, which comprises the following steps: enzymolysis: adding cellulase and pectinase into honeysuckle liquid, uniformly mixing and then carrying out enzymolysis; centrifuging: after the enzymolysis is completed, adding a culture medium for dissolving, and centrifuging by a centrifugal machine to obtain a clear honeysuckle flower leaching solution; performing primary sterilization; fermentation: inoculating a probiotic composition, and fermenting the honeysuckle lixivium; and performing secondary sterilization. The method has the advantages that the prepared honeysuckle flower fermentation liquor is high in alpha-glucosidase and pancreatic lipase inhibition capacity and high in chlorogenic acid content.