20 results about "Furanose" patented technology

The invention provides a lipidosome adjuvant system based on furanose type QS-21 saponin molecules and a preparation method. The lipidosome adjuvant system comprises lipidosome and the QS-21 saponin molecules, the lipidosome comprises a lipid bilayer formed by ionizable lipid, structural phospholipid and cholesterol, the QS-21 saponin molecule is in a furanose type and is loaded on the lipidosome, and the QS-21 saponin molecule is partially inserted into the lipid bilayer through hydrophobic triterpenoid aglycone of the QS-21 saponin molecule. The preparation method comprises the following steps: dissolving lipidosome components forming lipid bilayers in an organic solvent to form a lipid phase, dissolving furanose type QS-21 saponin molecules in an acidic aqueous phase buffer solution to form a QS-21 aqueous phase, mixing the lipid phase and the QS-21 aqueous phase, precipitating lipid, and self-assembling to form lipidosome nanoparticles loaded with furanose type QS-21 saponin molecules, and finally, purifying to remove the organic solvent and the unencapsulated QS-21 molecules. According to the invention, through well-designed lipid composition and a preparation process, furanose type QS21 molecules are stably integrated into the lipidosome.

This invention discloses an antitumor natural drug composition based on Norway maple and its preparation method, relating to the field of biomedical technology. The compound is selected from at least one of the following structures: 3-O-[β-D-glucopyranosyl-(1→4)-[β-D-galactopyranosyl-(1→2)]]-β-D-glucopyranoside-21-O-acetyl-22-O-angeloyl yrutile alcohol, with the molecular formula C2. 55 H 86 O 23 This invention isolates a class of triterpenoid saponins with a rutinol skeleton from the bark of Norwegian maple, enriching the chemical composition library of maple species. The compounds contain unique oligosaccharide chains and diacyl substitution patterns at C-21 and C-22. One compound has a rare branched acyl group (3-hydroxy-4-methylhexanoyl) at C-21, while the other compound contains an arabinofuranose unit in its C-3 trisaccharide chain. These structural features are rare in natural products and demonstrate excellent structural novelty.

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to an alpha-L-arabinofuranosidase mutant and application thereof. According to the invention, bacillus licheniformis BL-26 is mutated by NTG to obtain a mutated alpha-L-arabinofuranosidase gene, and then the mutated alpha-L-arabinofuranosidase gene is subjected to heterologous expression in pichia pastoris by means of genetic engineering to obtain a strain for high yield of alpha-L-arabinofuranosidase. The average fermentation enzyme activity in a 60 m < 3 > fermentation tank is 2741 U / mL, the optimal reaction pH is 4.5, and the optimal reaction temperature is 60 DEG C. The method is suitable for industrial production, the production cost of the alpha-L-arabinofuranosidase can be further reduced, and application and popularization of the alpha-L-arabinofuranosidase in the industrial fields of feed, food and the like are promoted.

This invention discloses a method for the targeted conversion and synthesis of rare ginsenosides Rg3 and Rh2. Specifically, fresh Panax notoginseng leaves are microwave-dried for 1-3 minutes to inactivate their endogenous β-glucosidase. The dried Panax notoginseng leaves are extracted with anhydrous methanol, and the extract is resuspended in an acetate-sodium acetate buffer solution after drying. α-arabinofuranase, β-xylosidase, and β-glucosidase are added to the resuspended solution, and an enzymatic reaction is carried out at 35-40℃ to directionally convert the original ginsenosides into rare ginsenosides Rg3 and Rh2. This method can achieve the inactivation of endogenous enzymes and the stable retention of original saponins in Panax notoginseng leaves in a very short time. Compared with the natural drying combined with exogenous enzymes, the conversion ratios of Rg3 and Rh2 are increased by 3.14 and 2.4 times, respectively. Compared with fresh Panax notoginseng leaves, the contents of Rg3 and Rh2 are increased by 20.6 and 8.7 times, respectively. This method is suitable for the high-value utilization of Panax notoginseng leaf waste resources and the large-scale preparation of rare saponins.

The present invention relates to a process for the synthesis of at least one C-glycoside comprising the following successive steps: (A) the introduction into a first mechanochemical reactor, separately or previously mixed, of at least one sugar in the form of pyranose and / or furanose and of the D and / or L series, said sugar having at least one hydroxyl function at the obligatorily free anomeric position, of a first reagent which is a β-diketone and of a base, in order to form a first initial mixture; (B1) at least a first grinding of said first initial mixture at a temperature greater than or equal to 20°C, in said first mechanochemical reactor, for a residence time less than or equal to 6 hours, so as to form a ketone C-glycoside; (C) the recovery at the outlet of the first mechanochemical reactor of a final mixture.The present invention also relates to the use of the mechanochemical reactor for synthesizing a C-glycoside or a C-glycoside derivative comprising said at least C-glycoside. Figure for the abstract: no figure.

The invention relates to the technical field of ionic liquid and biological medicine, in particular to multi-element biomass ionic liquid as well as a preparation method and a composition thereof. The name of the multi-element biomass ionic liquid is [N] XY, [N] represents a cation, X represents an anion cluster, and Y represents a hydrogen bond donor; the cation is derived from a compound containing a quaternary ammonium structure, the anion cluster is derived from amino acid and a derivative thereof, and the hydrogen bond donor is derived from pyranose and a derivative thereof or furanose and a derivative thereof. The compound can be used as a solvent and a stabilizer of indissolvable and unstable API (American Petroleum Institute), can completely replace a traditional volatile organic solvent, dissolves various indissolvable APIs, and reduces environmental and health damage caused by the traditional organic solvent.

The invention provides a synthesis method of 3-O-propargyl nucleoside, and belongs to the field of chemical preparation, 1, 2: 5, 6-bis-O-isopropylidene-alpha-D-isofuranose is used as a raw material, firstly propargyl is introduced through SN2 reaction under the action of strong base, and ether bonds are generated; secondly, removing acetonylidene in sequence through multi-step reaction, and generating ester groups at the same time; and finally, carrying out nucleophilic reaction on the basic group subjected to BSA silanization and carbohydrate ring iso-carbon under the action of a catalyst TMSOTf to form the nucleoside derivative. The synthesis method is simple in step, isomers are prevented from being generated, and the purity and quality of the product are improved.

The present application provides a method for one-pot biosynthesis of arabinonucleoside, and a composition. The method for one-pot biosynthesis of arabinonucleoside includes: mixing a substrate, uracil nucleoside phosphorylase or pyrimidine nucleoside phosphorylase, and purine nucleoside phosphorylase together, then performing biosynthesis, and directly obtaining arabinonucleoside through preparation by means of a one-pot method. The uracil nucleoside phosphorylase includes UP shown in SEQ ID NO: 1; the pyrimidine nucleoside phosphorylase includes PyNP shown in SEQ ID NO: 2; the purine nucleoside phosphorylase includes PNP shown in SEQ ID NO: 3; the substrate includes 1-beta-D-Arabinofuranosyluracil and a substrate base.

The invention relates to a method for improving the activity of arabinofuranosidase based on mutation modification and application. In particular to a method for improving the activity of arabinofuranosidase and a protein with the activity of arabinofuranosidase. The invention relates to a protein of arabinofuranosidase, a gene for coding the protein, a recombinant vector inserted with the gene, a transformant transformed with the gene, a method for preparing the arabinofuranosidase and application of the arabinofuranosidase in the field of feed additives. The two mutants prepared by the method disclosed by the invention still maintain relatively high residual enzyme activity at 70 DEG C and 80 DEG C, so that stable catalytic ability at high temperature is realized, and the two mutants have a relatively good application prospect in the field of preparation of feed additives.

The present application relates to a novel artificial nucleic acid, a method for producing the same, and a use thereof. The present application can be achieved by an artificial nucleic acid having a unit derived from a compound represented by the following formula (1) or a salt thereof: in the formula, Base is an optionally substituted aromatic heterocyclic group or an aromatic hydrocarbon ring group, A 1 is a single bond or an alkylene group, R 1 and R 2 are the same or different, and are a hydrogen atom, an optionally substituted alkyl group, an alkenyl group, a cycloalkyl group, a cycloalkenyl group, or an aryl group, a protecting group for a hydroxyl group, a phosphino group having a substituent, or an optionally substituted dihydroxyphosphinyl group or a hydroxylmercapto phosphinyl group, or R 1 and R 2 together with the adjacent 2 oxygen atoms and the carbon atoms at positions 3 to 5 of the furanose form an optionally substituted ring, R 3 is a hydrogen atom, an optionally substituted alkyl group, an alkenyl group, a cycloalkyl group, an aryl group, or an acyl group, a sulfonyl group having a substituent, or a silyl group, a functional molecular unit substituent, or a group represented by the formula: R 31 -X- (in the formula, R 31 is an optionally substituted amino group, X is an optionally substituted alkylene group, or at least one methylene group in the alkylene group is replaced with -N(R 32 ) - (in the formula, R 32 is a hydrogen atom or an alkyl group), -O-, or -S(=O) k - (in the formula, k is 0, 1, or 2), R 4 is a hydrogen atom, an optionally substituted alkyl group, or an aryl group, R 5 is a hydrogen atom or an optionally substituted alkyl group or an aryl group, R 4 and R 5 are not simultaneously a hydrogen atom, and n is 0 or 1.

The cellulose ester film contains one or more than one additive (A) and one or more than one additive (B), the additive (A) is a sugar ester compound containing at least one of 1-12 pyranose structures or furanose structures, and the additive (B) is a trimethylolpropane triphenyl ester compound containing condensation of tribenzoic acid and trimethylolpropane. According to the cellulose ester film disclosed by the invention, the mechanical strength and the dimensional stability of the film are improved by optimizing a formula and a two-way stretching design, and good structural integrity can still be kept in a high-temperature and high-humidity environment; the cellulose ester film disclosed by the invention has low moisture permeability, can effectively block moisture permeation, and can prevent a polarizer from being degraded in a high-temperature and high-humidity environment when being attached to the polarizer; the cellulose ester film provided by the invention has excellent dimensional stability, can maintain good dimensional stability under the conditions of large size and thinness, and effectively solves the problem that the cellulose ester film is easy to deform in a high-temperature and high-humidity environment.

This invention relates to a method for manufacturing an optical film, an optical film, a polarizer, and a liquid crystal display device. The objective is to provide a method for manufacturing a wide-width optical film with low retardation values ​​in both the in-plane and thickness directions, and to suppress display unevenness caused by environmental variations in the display device, using a plant-derived resin. The method for manufacturing the optical film of this invention is characterized by comprising: a step of forming a strip film from a cellulose ester resin containing a total acetyl substitution degree of 2.30 to 2.60 and a sugar ester having a furanose or pyranose structure; and a step of stretching said strip film, wherein the retardation value R... o The range is 0–10 nm, R t The process of stretching the long strip film, with a wavelength of -10 to 10 nm, includes the following steps: a first step to obtain a first stretched film; and a second step to further stretch the first stretched film to obtain a second stretched film, wherein the stretching temperature of the second step is in the range of 190 to 220°C.

The invention discloses alpha-L-arabinofuranosidase AraA02173 as well as a truncated body and application thereof, and belongs to the technical field of microorganisms. The invention provides amino acid sequences of AraA02173 and a truncated body thereof and a nucleotide sequence of a coding gene of the AraA02173. Characterization information is provided, and the characterization information includes that CBMs in the enzyme have a key maintaining effect on the temperature, pH stability and enzyme activity of an enzyme catalysis module of the CBMs. The purified enzymes of the AraA02173 and the truncation provided by the invention have obvious alpha-L-arabinofuranosidase activity, 4-nitrophenyl-alpha-L-arabinofuranoside can be degraded, the AraA02173 and the truncation also have beta-xylosidase activity, the specific enzyme activity of the AraA02173 is obviously superior to that of the truncation, and the AraA02173 and the truncation can be used for degrading 4-nitrophenyl-alpha-L-arabinofuranoside. Therefore, the alpha-L-arabinofuranosidase AraA02173 has great development potential, provides powerful theoretical support for preparing commercial enzyme preparations, and is of great significance in broadening related application of the alpha-L-arabinofuranosidase.

A composition comprising a flavor-providing substance and at least one norditerpenoid of Formula (I) is provided herein, (I) wherein R1, R2 and R3 are each independently selected from hydrogen, hydroxyl or oxo; wherein R4 is selected from methyl, hydroxymethyl, aldehyde, carboxylic acid or ester with C1-6 alkyl; wherein R5 is selected from hydrogen, C1-6 alkyl, a furanose moiety, a pyranose moiety or an oligosaccharide moiety having two to six saccharide moieties; and wherein R6 is selected from hydrogen, hydroxyl or C1-6 alkyl.

A composition comprising a flavor-providing substance and at least one norditerpenoid of Formula (I) is provided herein, (I) wherein R1, R2 and R3 are each independently selected from hydrogen, hydroxyl or oxo; wherein R4 is selected from methyl, hydroxymethyl, aldehyde, carboxylic acid or ester with C1-6 alkyl; wherein R5 is selected from hydrogen, C1-6 alkyl, a furanose moiety, a pyranose moiety or an oligosaccharide moiety having two to six saccharide moieties; and wherein R6 is selected from hydrogen, hydroxyl or C1-6 alkyl.

This invention relates to the field of directed biotransformation technology of fungal intracellular polysaccharides, disclosing a method for cultivating artificial Cordyceps militaris, a furan-type galactomannan, its preparation method, and its applications. The invention involves deep solid-state fermentation of Cordyceps militaris strains in a culture medium supplemented with an aqueous extract of a traditional Chinese medicine composed of Prunella vulgaris. During the fermentation process, alternating red, blue, and white light illumination is used to perform directed biotransformation of the component, ultimately obtaining artificially cultivated Cordyceps militaris fruiting bodies. Furan-type galactomannan is then purified to obtain the main chain. This furan-type galactomannan contains three residues: α-1,2-Manp-mannopyranose, 6-O-Me-α-1,3-Manf-mannofuranose, and α-1,2-Galf-galactofuranose. The O5 position of α-Manf and the O6 position of α-Galf on the side chains are replaced by T-β-D-Manf terminal mannofuranose, exhibiting significant immunomodulatory effects and can be used as an immunomodulator or for the preparation of immunomodulators.

The invention provides a hemicellulase recombinant aspergillus niger composition and application thereof, and belongs to the technical field of recombinant microorganisms. The problem of degrading natural fiber materials is solved. The recombinant aspergillus niger comprises a recombinant aspergillus niger composition, and the recombinant aspergillus niger composition takes aspergillus niger TH-2-D as a starting strain, aspergillus niger endogenous xylanase xynB is respectively connected with penicillium oxalicum xylanase xyn3, penicillium oxalicum xylanase xyn4 or penicillium oxalicum arabinofuranosidase arab2 to obtain xynB-xyn3, xynB-xyn4 and xynB-arab2 recombinant genes, and the xylanase xyn3, the xynB-xyn4 and the xynB-arab2 recombinant genes are used for preparing the recombinant aspergillus niger composition. And replacing the saccharifying enzyme gene glaA with the recombinant gene to obtain the recombinant aspergillus niger composition. The method is mainly used for degrading hemicellulose.

The invention relates to a method for determining α-arabinofuranosidase (ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive having α-arabinofuranosidase activity, said α-arabinofuranosidase activity to be determined being specific to said additive, said method comprising the following steps: a sample, referred to as the control sample (c), of said product free from said additive is available on the one hand, and said sample to be analyzed (p) is available on the other hand; for each of said control (c) and sample to be analyzed (p), respectively, an enzymatic fraction having α-arabinofuranosidase activity is extracted under the same conditions, then the α-arabinofuranosidase activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp – ABFc is calculated to obtain said ABF activity specific to said additive in said product;according to which method, α-arabinofuranosidase activity is measured by fluorescence using an enzymatic substrate, 4-methylumbelliferyl-α-L-arabinofuranoside (4-MU-ABF), which is hydrolyzable into fluorescent 4-methylumbelliferone (4-MU).

The application provides a flavone glycoside with antiviral activity and a preparation method and application thereof. The structural formula of the flavone compound phloretin 3'-C-(2-O-trans-p-coumaroyl-3-O-beta-D-glucosyl)-beta-D-fucosyl-6'-O-(2-O-beta-D-fucosyl)-alpha-L-arabinofuranoside of the application is shown as formula (I), and the compound is isolated from dried or fresh products of fruits, stems or leaves of Averrhoa carambola. In vitro pharmacological experiments prove that the compound phloretin 3'-C-(2-O-trans-p-coumaroyl-3-O-beta-D-glucosyl)-beta-D-fucosyl-6'-O-(2-O-beta-D-fucosyl)-alpha-L-arabinofuranoside has good anti-H1N1 virus ability, and the EC 50 value thereof is 8.916 muM, indicating that the compound has good antiviral activity. The flavone compound phloretin 3'-C-(2-O-trans-p-coumaroyl-3-O-beta-D-glucosyl)-beta-D-fucosyl-6'-O-(2-O-beta-D-fucosyl)-alpha-L-arabinofuranoside is expected to be used as a lead compound to develop a new antiviral drug.

The invention relates to a method for determining α-arabinofuranosidase (abbreviated ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive that exhibits ABF activity, the ABF activity to be determined being specific to the additive, the method comprising the following steps: on the one hand, a sample, referred to as the control sample (c), of the product which is free of the additive, and, on the other hand, the sample to be analyzed (p), are provided; for each of the control sample (c) and the sample to be analyzed (p), respectively, an enzyme fraction exhibiting ABF activity is extracted under the same conditions, then the ABF activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp - ABFc is calculated to obtain the specific ABF activity of the additive in the product; according to which method the ABF activity is measured by fluorescence by means of a substrate for the enzyme, 4-methylumbelliferyl-α-L-arabinofuranoside, which is hydrolyzable to fluorescent 4-methylumbelliferone.