168 results about "Glycoside hydrolase" patented technology

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.

The invention relates to biological complex enzyme toothpaste and a preparation method thereof. The toothpaste is prepared from the following components by weight percentage: 5-10% of sorbierite, 15-20% of hydrous silica, 10-20% of aluminum hydroxide, 10-20% of glycerin, 2-3% of sodium lauroyl sarcosine, 1-1.3% of flavor, 0.8-1.2% of cellulose gum, 0.3-0.6% of carrageenin, 0.3-0.5% of droxyethylcellulose, 0.5-1.2% of sodium phytate, 0.15-0.3% of saccharin sodium, 0.3-0.5% of strontium chloride, 0.1-0.3% of trisodium phosphate, 0.00003-0.00008% of staphylococcus lysozyme, 0.00005-0.00015% of muramidase, 0.00001-0.00004% of proteolytic enzyme, 0.00002-0.00005% of glycosidic hydrolase, 0.00002-0.00005% of peroxydase, 0.08-0.15% of borneol, 0.1-0.15% of borax, 0.08-0.1% of vermilion, 0.1-0.15% of natrii sulfas exsiccatus, 0.05-0.1% of triclosan, 0.1-0.4% of allantoin, and the balance of water. The total weight percentage of the components is 100%. The biological complex enzyme toothpaste has complete function.

The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

The invention relates to a method for preparing konjac glucomannan and oligo-glucomannan with different molecular weights. The method comprises the following steps of: rapidly adding0.1-30 parts of purified konjac glucomannan into a solution prepared by 0.001-0.01 part of glycoside hydrolase and 10-100 parts of distilled waterunder the stirring condition; reacting for 0.5-72 hours under the condition of the temperature of 30-60 DEG C and the pH of 2.0-10.0; and deactivating enzyme for 15 minutes in a boiling water bath to obtain konjac glucomannan and oligo-glucomannan aqueous solutions with different molecular weights; adding 1-30 parts of peptizing agent for stirring for 0.5-5 hours; depositing konjac glucomannan and oligo-glucomannan molecules with different molecular weights with 10-100 parts of anhydrous ethanol; after centrifugally separating sediments, adding 10-100 parts of anhydrous ethanol for strongly stirring for 0.5-5 hours; repeatedly depositing, centrifuging and stirring for 3-5 times, drying the sediment obtained by last centrifugation in a double-conic vacuum rotary drier; and grinding and screening the sediment to obtain the konjac glucomannan and the oligo-glucomannan. The method disclosed by the invention has the advantages of simplicity, low cost and wide product application.

The invention provides a production method, which can enable the content of Phenylethanoid Glycoside combination before and after the processing to keep stable through improving the processing treatment technology of a broomrape decoction piece, wherein, the Phenylethanoid Glycoside combination is the active ingredient abundant in broomrape. Through a high-frequency microwave electrical field, the invention leads a polar molecule in a succulent stem cell of a waterrich fresh broomrape to perform high-frequency swing, vibration, and intermolecular friction and extrusion to cause the inner and surface temperature of a broomrape cell to rise rapidly; the pyrometric effect for inactivating the enzyme activity, so as to effectively block and restrain the degradation loss of the Phenylethanoid Glycoside combination caused by the enzymatic reaction induced by enzyme systems such as hydrolytic ferment, glucoside hydrolase, etc., abundant in a fresh broomrape cell; at the same time, the broomrape decoction piece is dried through the technology for microwave drying or other drying methods. The analysis determination shows that the content of echinacoside and a verbascoside monomer compound which are the main index components for weighing the quality of the broomrape decoction piece in the acquired broomrape decoction piece can reach 13 percent to 24 percent (by dry matters), and the water content is 1.3 percent to 4.1 percent.

The invention relates to the field of tobacco additives, in particular to a preparation method and an application of a fruit flavor extract. The preparation method includes the steps: crushing fresh plant fruits to prepare into slurry; adding compound enzyme into the slurry to hydrolyze, and performing purification and concentration for hydrolysis products to obtain the fruit flavor extract. The compound enzyme comprises protease and glycoside hydrolase, and the weight ratio of the protease to the glycoside hydrolase is 1: (15-60). According to the preparation method, enzymolysis is performed for fresh fruits by the aid of specific biological enzyme, walls are effectively and rapidly broken, macromolecular substances are transformed, and impurities are removed. When the obtained fruit flavor extract is used for cigarettes, cigarette flavors can be richened, sweet feeling is increased, moisture retention of cigarette senses is achieved, comprehensive evaluation indexes are good, and the preparation method is simple in preparation process, wide in application range and applicable to large-scale popularization and application.

The invention relates to an alkalic xylanase gene and an engineering bacterium containing the same. The alkalic xylanase gene has the overall length of 687 bp, is called XynG1-3, encodes 228 amino acids and predicts that the molecular weight of the amino acids is 25444Da, wherein the first 27 amino acids are signal peptides of the alkalic xylanase gene, and 38th-221st amino acids are conserved sequences of a glycoside hydrolase GH11 family. Compared with a reported xylanase gene sequence, individual nucleotides of the obtained and expressed alkalic xylanase gene are different; the optimal operative temperature and the optimal pH of the recombinase are respectively 55 DEG C and 8.0, relative enzyme activity is more than 50 percent after preserving heat at the pH of 9.0 and 60 DEG C for 1 hour, and the relative enzyme activity is more than 70 percent after preserving the heat under the conditions of the pH of 6-9 and the temperature of 55 DEG C for 1 hour.

The invention provides a microbial strain aspergillus.aculeatus (CGMCCNo.3876) used for efficiently extracting polydatin and analogues thereof from plant materials such as polygonum cuspidatum, peanut shells, grape skins and the like, and a complex enzyme prepared by fermenting the microbial strain. The complex enzyme of the strain is used for extracting polydatin and analogues thereof by an enzyme method, and the extraction ratio is 2.3%. The extraction ratio of the complex enzyme is improved by 91.7% through treatment compared with the exoenzyme (control) to which the strain aspergillus.aculeatus is not added. The ratio of conversion from polydatin to resveratrol by using the complex enzyme of the strain is above 99.5%. The polygonum cuspidatum glycoside hydrolase purified from the complex enzyme of the strain is used for converting 98% of polydatin to resveratrol, and the conversion ratio is 99.7%. The purity of resveratrol is 98.5%. By the invention, a novel complex enzyme preparation is actually provided for production, the problems of low catalytic activity of enzymes, low conversion ratio, overlong time and high cost in current production upgrading are fundamentally solved, and the production practice application is promoted.

The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention relates to a glucoside hydrolase family 61 protein gene. The nucleotide sequence of the glucoside hydrolase family 61 protein gene is shown as SEQ ID No. 1. The invention relates to a protein of the glucoside hydrolase family 61 protein gene, and the amino acid sequence of the protein is shown as SEQ ID NO. 2. The glucoside hydrolase family 61 protein gene can further utilize pET26 plasmids to structure recombinant plasmids and converts the recombinants plasmids into Escherichia coli expression strains, thereby achieving soluble expression of expression products. The invention also provides preparation and purification methods of the protein. The preparation and purification methods can help obtain recombinant glucoside hydrolase family 61 proteins with a degree of purity upto higher than 95%; the prepared recombinant glucoside hydrolase family 61 proteins have enzymatic activity for hydrolyzing sodium carboxymethylcellulose to produce glucose, belong to glucoside hydrolase family 61 enzymes and can be matched with other types of cellulases during different types of application, preferably, be combined with one or more beta-glucosidases or endoglucanases.

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as Gux1. Gux1 has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified Gux1. Methods of making and using Gux1 polypeptides, including fusions, variants, and derivatives, are also disclosed.

The invention relates to the field of tobacco additives, in particular to a preparation method and an application of a fruit flavor extract. The preparation method includes the steps: crushing fruit raw materials, extracting in an ethanol refluxing manner, filtering mixture, and taking filter residues; adding compound enzyme to hydrolyze, and performing purification and concentration for hydrolysis products to obtain the fruit flavor extract. The compound enzyme comprises protease and glycoside hydrolase, and the weight ratio of the protease to the glycoside hydrolase is 1: (15-60). According to the preparation method, refluxing and extracting are firstly performed, enzymolysis is secondly performed, lipid materials releasing irritating odor are removed, walls are effectively and rapidly broken, macromolecular substances are transformed, and impurities are removed. When the obtained fruit flavor extract is used for cigarettes, cigarette flavors can be richened, sweet feeling is increased, moisture retention of cigarette senses is achieved, comprehensive evaluation indexes are good, and the preparation method is simple in preparation process, wide in application range and applicable to large-scale popularization and application.

The invention relates to a nucleotide sequence of encoded fucoidin glucoside hydrolase and an application thereof. The nucleotide sequence is SEQ ID NO.1, encoded protein contains 529 amino acids, the nucleotide sequence is SEQ ID NO.2, and the predicted molecular mass of protein is 58.8 kDa. According to the nucleotide sequence and the application thereof, the gene GenBank No:AJ877239 of the encoded fucoidin hydrolase is subjected to codon optimization, 1599bp basic groups exist after optimization, and are loaded into a cloning vector pET28a, genes of the optimized fucoidin hydrolase can be expressed in escherichia coli BL21, glucoside hydrolase capable of efficiently and exclusively hydrolyzing fucoidin is obtained, and a biological degrading enzyme is provided for fucoidin hydrolysis.

In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.