Method for extracting oxidized resveratrol

A technology for oxidizing resveratrol and an extraction method, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as no research reports, and achieve the effects of simple process, convenient operation and product safety

Inactive Publication Date: 2009-12-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Get the skin of fresh mulberry branches, dry it naturally, and crush it to 80-120 mesh;

[0014] (2) adding the mulberry powder to the ethanol solution with a concentration of 30% by mass percentage, then adding glycoside hydrolase, the mass volume ratio of the mulberry powder and the ethanol solution is: 1: 10, the mass of the mulberry powder and the glycoside hydrolase The ratio is: 100:1, stir evenly, seal and enzymatically extract at 47°C for 72 hours;

[0015] (3) Filter the reaction solution after enzymolysis, concentrate the filtrate and put it on a 100-mesh polyamide chromatography separation column, first elute with an ethanol solution with a mass percentage concentration of 70%, then elute with 100% ethanol, and collect 100% ethanol The eluent of ethanol was concentrated to obtain the crude product of oxidized resveratrol, its purity was 44%, and the recovery rate of ethanol was 78%;

[0016] (4) Dissolve the above-mentioned oxidized resveratrol crude pro...

Embodiment 2

[0018] (1) Get the skin of fresh mulberry branches, dry at 42°C, and pulverize to 80-120 mesh;

[0019] (2) Adding the mulberry powder into the ethanol solution with a mass percent concentration of 50%, then adding glycoside hydrolase, the mass-to-volume ratio of the mulberry powder and the ethanol solution is 1: 5, the mass of the mulberry powder and the glycoside hydrolase The ratio is: 10:1, stir evenly, seal and enzymatically extract at 45°C for 48 hours;

[0020] (3) Filter the reaction solution after enzymolysis, concentrate the filtrate and put it on a 60-mesh polyamide chromatography separation column, first elute with an ethanol solution with a mass percentage concentration of 60%, then elute with 100% ethanol, and collect 100% ethanol The eluent of ethanol is concentrated to obtain the crude product of oxidized resveratrol, its purity is 40%, and the recovery rate of ethanol is 60%;

[0021] (4) Dissolve the above-mentioned oxidized resveratrol crude product in hot ...

Embodiment 3

[0023] (1) Take the skin of the fresh mulberry root, dry it naturally, and crush it to 80-120 mesh;

[0024] (2) adding the mulberry powder to the ethanol solution with a concentration of 60% by mass percentage, then adding glycoside hydrolase, the mass-to-volume ratio of the mulberry powder and the ethanol solution is 1: 15, the mass of the mulberry powder and the glycoside hydrolase The ratio is: 80:1, stir evenly, seal and enzymatically extract at 46°C for 12 hours;

[0025] (3) Filter the reaction solution after enzymolysis, concentrate the filtrate and put it on a 300-mesh polyamide chromatography separation column, first elute with 90% ethanol solution with a mass percentage concentration, then elute with 100% ethanol, and collect 100% ethanol The eluent of ethanol is concentrated to obtain the crude product of oxidized resveratrol, its purity is 60%, and the recovery rate of ethanol is 75%;

[0026] (4) Dissolve the above-mentioned oxidized resveratrol crude product in...

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Abstract

The invention relates to a method for extracting oxidized resveratrol. The oxidized resveratrol is directly extracted from mulberry barks (mulberry branch barks or mulberry root barks) through enzymolysis. The method comprises the following steps: peeling off barks of fresh mulberry branches or mulberry roots first; drying the barks, and smashing the barks; performing enzymolysis on the smashed barks by using ethanol solution added with glucoside hydrolase; filtering and concentrating the mixture; putting the mixture on a polyamide chromatographic column to perform gradient elution with ethanol; and recovering the eluant to obtain a product. The method breaks through the conventional pure alcohol extraction or pure water extraction, combines enzymatic conversion with extraction and has simple process and convenient operation; the technical process has no solvent which are poisonous to human bodies; and the obtained product is safe and is suitable for industrialized production. The content of the product of the oxidized resveratrol extracted by the method is between 95 and 98 percent, and the recovery percent of the ethanol is between 60 and 80 percent.

Description

technical field [0001] The invention relates to the technical field of substance extraction, in particular to a safe, efficient and rapid extraction and purification method for oxidized resveratrol. Background technique [0002] Oxyresveratrol (Oxyresveratrol) is polyhydroxystibene (PHS), which belongs to stilbene compounds. In recent years, a large number of studies have found that, in addition to the long-known antibacterial effects, this type of active ingredient can expand capillaries to improve microcirculation, lower blood lipids, lower blood sugar, anti-tumor, inhibit allergic reactions, regulate the body's immune defense system, anti-thrombosis, Whitening and anti-oxidation effects. Most of the activities of stilbene compounds are based on anti-oxidation, and their contribution comes from the phenolic hydroxyl groups in the structure. Therefore, the number and substitution position of phenolic hydroxyl groups are very important. Oxidized resveratrol has four phenol...

Claims

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Application Information

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IPC IPC(8): C12P7/22C07C39/21C07C37/70
Inventor 徐立伍春刘峻池余茂德夏庆友
Owner SOUTHWEST UNIVERSITY
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