739 results about "Hydrolase" patented technology

The invention relates to the technical field of microorganisms, in particular to a culture medium and a method for culturing Ackermania muciniphila for activating Amuc-1260 by using lambda-carrageenan as a carbon source, according to the culture method, the lambda-carrageenan is used for replacing a traditional carbon source, thallus growth and proliferation can be remarkably promoted, the effect is optimal under the condition of 2.00 g / L, and the culture cost is low. Compared with the conventional carbon source (glucose), the carbon source is improved by 92.91%. Further research finds that the lambda-carrageenan can induce the Akk bacteria to up-regulate the expression of glycoside hydrolase such as Amuc-1260 and enhance the activity of pathways such as glycosaminoglycan degradation, amino acid metabolism and insulin signals, so that the metabolic adaptability and energy utilization efficiency of the bacteria are improved. Meanwhile, the Akk bacteria secrete more functional short-chain fatty acids such as acetic acid, propionic acid and the like under the condition, and have the effects of regulating intestinal health and maintaining metabolic homeostasis. The invention provides a new theoretical basis and technical support for large-scale culture of Akk bacteria and development of a metabiotic product.

This invention relates to the field of water treatment technology, specifically to a reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme, and its application. Its main purpose is to remove biofouling from reverse osmosis membranes and restore membrane flux. This patent discloses a novel reverse osmosis membrane cleaning agent formulation. Considering the different pH requirements of the cleaning agent in actual production, different types of bioactive hydrolytic enzymes are selected to disrupt the structure of bacterial cell walls and their extracellular polymers. A reducing agent and a non-oxidizing bactericide are combined to enhance the cleaning effect of the reverse osmosis membrane. For different cleaning purposes, non-oxidizing bactericides suitable for acidic and alkaline cleaning are formulated. Sodium benzoate and benzalkonium chloride show good application prospects in the removal of reverse osmosis membrane fouling.

The invention relates to a method for detecting activity of gamma-polyglutamic acid hydrolase and application. The method comprises the following steps: (1) preparing an enzymolysis reaction solution and a blank reaction solution; (2) preparing an NAC / OPA mixed solution; (3) adding an NAC / OPA mixed solution into the enzymatic hydrolysis reaction solution to obtain a reaction solution to be detected; (4) drawing a standard curve by taking L-glutamic acid as a standard substance; and (5) detecting the fluorescence intensity of the blank reaction solution and the reaction solution to be detected by using a microplate reader, and then calculating the activity of the gamma-polyglutamic acid hydrolase according to the fluorescence intensity and the standard curve. The method for detecting the activity of the gamma-polyglutamic acid hydrolase is high in detection speed, detection of a single sample can be completed within 25 min, and nearly real-time process monitoring is achieved. And the method has the advantages of reliable detection result and high accuracy, can be used for monitoring the enzyme activity of the gamma-polyglutamic acid hydrolase in the gamma-polyglutamic acid preparation process in real time and guiding the optimization of the fermentation process, and has extremely high industrial application value.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses keratinase Phyto-Keratinase and application of the keratinase Phyto-Keratinase. The keratinase Phyto-Keratinase comprises the following components: (1) protease which is coded by a nucleotide sequence as shown in SEQ ID NO.1; and / or (2) protease which is derived from bacillus subtilis, is coded by a nucleic acid sequence with more than 98% of identity with the SEQ ID NO.1 nucleotide sequence and has the characteristic of hydrolyzing keratin. According to the plant keratinase Phyto-Keratinase disclosed by the invention, the Phyto-Keratinase (plant keratinase) produced by utilizing microbial fermentation is a serine proteolytic enzyme, is also a biocompatible cutinase, and is obtained by fermenting saccharomyces cerevisiae. Phyto-Keratinase provides a nitrogen source for the growth of plants (beans), and contains various amino acids (functional peptides) and special proteolytic enzymes derived from the plants at the same time. The Phyto-keratinase can provide the effects of resisting inflammation, removing cutin and providing nutrition for skin regeneration at the same time, can relieve various inflammations by inhibiting PAR-2 receptors, and has potential value in the fields of cosmetics, beauty medicine and environment.

The present invention relates to novel carbamate hydrolases for the enzymatic decomposition of polyurethanes and to an enzymatic process for the complete decomposition of polyurethanes into defined monomers.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The present invention relates to a process for the preparation of substituted 4-(N′-hydroxy-carbamimidoyl)benzoic acids, which can be obtained by nitrilase catalyzed hydration of substituted terephthalonitriles of formula (II) in an aqueous medium to afford (ammonium) 4-cyanobenzoic acids (IIa). The hydration is followed by treatment of the aqueous reaction medium with hydroxylamine or a salt thereof to afford amidoximes (I).

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention discloses an SAM hydrolase mutant and application thereof, the specific activation effect of halogen anions (F <->, Cl <->, Br <-> and I <->) on SAM hydrolase (containing MJ1651 wild type and mutant) is found for the first time, and the active sequence of the SAM hydrolase is F <-> > I <-> > Br <-> = Cl <->. According to the method, 400 mM of halide salt is added into a reaction system, so that the catalytic efficiency is improved in a breakthrough manner; the yield of wild type enzyme adenosine is improved by 20-300% (optimally, an F-system); the yield of the mutant R89C is soaring up by nearly 6 times under the action of KF; the activity of the immobilized enzyme is still more than 90% after the immobilized enzyme is repeatedly used for 5 times. The method is easy and convenient to operate, green production is achieved with water as the solvent (three wastes are reduced by 90%), the comprehensive cost is reduced by 41%-80 / kg, and an efficient solution is provided for industrial biological manufacturing of antiviral drug intermediates and the like.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention discloses a method for improving the quality of a Tieguanyin tea extracting solution through segmented composite enzymolysis extraction, and belongs to the technical field of tea drink preparation. The method comprises the following steps: S1, performing low-temperature aroma extraction on Tieguanyin dry tea, and performing crushing so as to obtain crushed tea; s2, adding water and beta-glucosidase into the broken tea to carry out first-stage enzymolysis extraction, and separating to obtain tea aroma extract liquid and aroma-free tea residues; s3, water and composite hydrolase are added into the aroma-free tea residues for second-stage enzymolysis extraction, separation and filtration are performed, and a hydrolysis extraction solution is obtained; and S4, mixing the tea fragrance extract liquid with the hydrolysis extract liquid, cooling, centrifuging and filtering to obtain the Tieguanyin tea extract liquid. According to the method disclosed by the invention, the original flavor of the Tieguanyin is maintained, meanwhile, the fragrance and the mouth feel are remarkably improved, and the problems of fragrance loss and bitter taste caused by traditional high-temperature long-time extraction and cooked soup taste generated by tannase enzymolysis are avoided.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

This invention provides a nitrile hydrolase mutant and its application in the synthesis of p-cyanobenzoic acid. The invention provides a nitrile hydrolase mutant that enhances the catalytic activity for the synthesis of p-cyanobenzoic acid. The mutant protein is a non-natural protein, and it exhibits significantly enhanced activity in catalyzing the conversion of terephthalonitrile to p-cyanobenzoic acid. Furthermore, the mutant protein contains mutations in two or more core amino acids related to the enzyme's catalytic activity in the wild-type nitrile hydrolase. The nitrile hydrolase mutant of this invention can significantly improve the yield of the synthesized product catalyzed by the nitrile hydrolase.

The invention relates to the technical field of biology, and discloses an efficient extraction process of SOD in silphium perfoliatum, which comprises the following steps: mixing a silphium perfoliatum superoxide dismutase crude extract, polyethylene glycol and a first salt, and adjusting the pH value to form an aqueous two-phase system to enrich SOD; adding polysaccharide hydrolase and protease into the system to carry out in-situ enzymolysis so as to degrade macromolecular impurities; after the enzymolysis is finished, adding a second salt for competitive distribution, and selectively removing the added functional enzyme to a salt phase; and finally, separating and collecting a supernatant phase rich in superoxide dismutase. By integrating aqueous two-phase extraction, in-situ enzymolysis and competitive distribution into a single reaction system, a tedious intermediate separation unit in a traditional process is replaced, and by continuous and targeted removal of endogenous macromolecular impurities and exogenous functional enzymes, the purity and specific activity of a final product are remarkably improved, so that the method is suitable for industrial production. And the biological activity of SOD is effectively protected by the mild separation conditions in the whole process.

The invention discloses a nicotinic acid immobilized enzyme conversion method, which comprises the following steps: taking wet thalli containing nitrilase, preparing a flocculation clear liquid, passing the flocculation clear liquid through an ultrafiltration membrane, carrying out embedding immobilization to obtain embedded particles not greater than 2mM, and after the embedded particles are hardened, separating and purifying the obtained conversion liquid by adopting a membrane separation method to obtain the nicotinic acid immobilized enzyme. Sequentially carrying out microfiltration, activated carbon decoloration, ultrafiltration, nanofiltration decoloration, acidification crystallization and centrifugation to finally obtain a nicotinic acid finished product. The immobilized enzyme prepared by the method disclosed by the invention can stably exist in nitrile substances, can be repeatedly used for many times, and is high in stability and low in enzyme activity loss rate, and a separated and purified product is free of impurities and high in yield.

The invention relates to an antibody drug conjugate as well as a preparation method and application thereof, the preparation method of the antibody drug conjugate comprises the following steps: S10, obtaining a wild type antibody, and carrying out hydrolysis reaction on galactose at the tail end of a sugar chain of the wild type antibody to obtain an antibody of which the tail end is four GlcNAc sugar types; s20, obtaining B4GALT1 (Y286L), mixing the UDP-GalNAz, the B4GALT1 (Y286L) and the antibody, and carrying out an enzymatic reaction so as to transfer the azido galactose to the tail end of a carbohydrate chain of the antibody, so as to obtain a carbohydrate chain modified antibody; and S30, mixing a target drug with the carbohydrate chain modified antibody, and carrying out a one-step click chemical reaction to obtain the antibody drug conjugate. The galactose hydrolase can retain four GlcNAc sites, and after the galactose hydrolase is connected with GalNAz, four target drug molecules can be combined, the DAR value of the antibody drug conjugate is increased, and the drug activity of the antibody drug conjugate is improved.

The application discloses an animal bifidobacterium producing bile salt hydrolase and application thereof in neonatal jaundice, and belongs to the technical field of microorganisms. The animal bifidobacterium lactis WXM screened by the application has the effect of relieving neonatal jaundice, can significantly improve weight loss of a jaundice individual in an early life stage, improve abnormal increase of a bilirubin level in serum, improve liver damage, improve the level of an enzyme related to liver function, the expression level of a related inflammatory factor in the liver and the expression level of an enzyme UGT1A1 related to bilirubin metabolism, and has the effect of relieving neonatal jaundice. The strain of the application has high safety and has wide application value in the fields of food, functional food, health products, medicine and the like.

A peptidoglycan hydrolase LysPH1 having a broad-spectrum lytic activity, and a mutant and the use thereof, which belong to the technical field of biological formulations. An antibacterial peptide LysPH1-CP, a peptidoglycan hydrolase LysPH1 containing the antibacterial peptide LysPH1-CP and a peptidoglycan hydrolase mutant LysPH1-K containing the peptidoglycan hydrolase LysPH1 all have good thermal stability, and can broadly lyse both Gram-negative bacteria and Gram-positive bacteria. Moreover, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K can be solubly expressed in Escherichia coli, and have high enzymatic activity. Therefore, the antibacterial peptide LysPH1-CP, the peptidoglycan hydrolase LysPH1 and the peptidoglycan hydrolase mutant LysPH1-K have good application prospects in the preparation of an anti-infective drug and the research and development of feed additives.

The invention discloses an oxidoreductase mutant and application thereof, and belongs to the field of enzyme engineering. The invention provides a recombinant oxidoreductase HbKR mutant which is used for catalytically producing (R)-decalactone. The oxidoreductase HbKR mutant disclosed by the invention realizes soluble expression in Escherichia coli BL21 (DE3), and the oxidation activity of the oxidoreductase HbKR mutant is improved by 4.61 times compared with that of a parent enzyme. An oxidoreductase HbKR mutant and lactone hydrolase Rpa3624 derived from Rhodopseudomonas palustris are combined for use, reaction is performed for 0.5 h under the conditions that the temperature is 35 DEG C and the pH value is 10.5, compared with a wild type, the yield of (R)-delta-decalactone is increased to 48.2% from 42.3%, and the stereoselectivity is increased to 98.7% from 94.2%.

The present application belongs to the technical field of enzyme engineering and plastic degradation, and particularly relates to an esterase, a preparation method thereof and application thereof in plastic degradation. Genome sequencing analysis is performed on a screened polyester plastic degrading strain, and an esterase 8GL004290 is successfully screened, which belongs to the alpha / beta hydrolase family. It is verified through experiments that the polyester degrading enzyme provided by the present application can not only degrade polyester model small molecule substrates, but also exhibits good degradation capacity for different polyester plastics. The esterase 8GL004290 has the highest degradation activity on a polyurethane substrate, and the substrate types are widely covered. Therefore, the present application has extremely important application value for the biodegradation treatment of mixed plastic waste.

Use of a bitter melon exosome and an oral medicament prepared therefrom. The bitter melon exosome obtained from the bitter melon juice can be used for preparing a pharmaceutical formulation embedding a protein, a polypeptide, a small molecule peptide, a nucleic acid, or a small molecule compound. The compositions of the oral medicament of the present invention comprise the bitter melon exosome and an active pharmaceutical ingredient encapsulated in the bitter melon exosome. The bitter melon exosome is suitable for preparing a drug that is easily damaged by the gastrointestinal tract and is mostly administered by means of an injection route into an oral agent, and the oral agent can produce a similar therapeutic effect to that of the injection administration route by oral administration, and is convenient to use. The extraction method for the bitter melon exosome is simple and has a low cost. Moreover, the bitter melon exosome has no immunogenicity, has relatively high biocompatibility and biosafety, and has relatively strong tolerance to gastric acid and digestive tract proteolytic enzymes, and intestinal barrier permeability.

The present invention relates to a method for hydrolyzing carbohydrates present in raw materials containing carbohydrates, comprising the step a) of contacting the raw material containing carbohydrate with a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a medium in which bacteria capable of secreting carbohydrate-hydrolyzing enzymes have been fermented, to a method for simultaneously liquifying and saccharifying starch, a method for simultaneously liquifying, saccharifying and fermenting starch, and to a method for producing a biomass, the method comprising the step of cultivating bacteria secreting carbohydrate-hydrolyzing enzymes on a medium comprising complex carbohydrates as a major carbon source.

The invention discloses polyethylene glycol terephthalate hydrolase PmPETase and application thereof.The hydrolase PmPETase is protein with the amino acid sequence shown in SEQ ID NO.1. An expression system suitable for the PmPETase is constructed, recombinase protein with catalytic activity is obtained, and the polyethylene glycol terephthalate hydrolase PmPETase can be used for preparing the polyethylene glycol terephthalate hydrolase PmPETase. The substrate specificity, the catalytic property and the potential plastic degradation capacity of the PET degrading enzyme are comprehensively analyzed, and a new theoretical basis and application reference are provided for development and utilization of the PET degrading enzyme and a high polymer material green treatment technology.

A first aspect of the invention relates to a method of treating or preventing tissue damage, or treating or preventing tissue destruction, in a subject, said method comprising administering to the subject a therapeutically or prophylactically effective amount of a soluble epoxide hydrolase (sEH) inhibitor. A further aspect of the invention relates to a method of treating or preventing leishmaniasis in a subject, said method comprising administering to the subject a therapeutically or prophylactically effective amount of a soluble epoxide hydrolase inhibitor.

The invention provides a toxoplasma gondii gene deletion strain with an immune protection effect and application of the toxoplasma gondii gene deletion strain, and belongs to the technical field of veterinary parasitology, veterinary immunology and parasite molecular biology. According to the toxoplasma gondii gene deletion strain, a gamma-glutamyl hydrolase gene is deleted. The invention also provides a method for constructing the insect strain. The method comprises the following steps: constructing a circular plasmid pSAG1-Cas9-U6-sgGGH; constructing a repair template containing upstream and downstream homologous arms of the gamma-glutamyl hydrolase gene and a DHFR resistance gene; co-transfecting the annular plasmid and the repair template to a toxoplasma gondii ME49 strain; performing drug screening on pyrimethamine to obtain positive monoclone; and confirming that the GGH gene is completely knocked out through PCR (Polymerase Chain Reaction) detection, sequencing analysis and immunofluorescence detection. The toxoplasma gondii gene deletion strain can provide a remarkable protection effect on toxoplasma gondii infection and is high in safety.

This invention discloses a mutant of raw starch hydrolase with improved stability and its applications. Using α-amylase AmyZ2 as the starting enzyme, and through computer-aided design to clarify the mutation site, a mutant was heterologously expressed in Bacillus subtilis, resulting in an α-amylase with improved stability. When using raw corn starch as a substrate, the mutant exhibits 1.57 times the specific enzyme activity and 2 times the stability of the starting enzyme. While maintaining the specific enzyme activity, the mutant enzyme shows a significant improvement in thermostability. This mutant has potential application value in the production of starch sugars using raw corn starch as a substrate.

The application discloses a kind of PET hydrolytic enzyme of deep learning algorithm design and preparation method thereof, it is related to biotechnology field, preparation method includes selecting polyester hydrolytic enzyme PHL7, learns protein crystal structure with deep learning algorithm InstructPLM, utilizes Esmfold to carry out three-dimensional structure modeling, carries out molecular docking with candidate protein and PET, carries out molecular dynamics simulation, and good protein is screened out with substrate binding;Express, purify;Obtain hydrolytic enzyme HN and / or hydrolytic enzyme JW, amino acid sequence is as shown in SEQ ID NO:1 and / or SEQ ID NO:2;And application of novel PET hydrolytic enzyme in hydrolysis PET.The application can simply, quickly and efficiently screen out PET hydrolytic enzyme from large quantities of generated candidate sequences by screening process, and expands the source of PET hydrolytic enzyme.