40 results about "Cellobiohydrolase activity" patented technology

Polypeptides having the amino acid sequence represented by SEQ ID NO:1 or derived therefrom by at least one of deletion, addition, insertion or substitution of one or more amino acids in the above sequence and showing a cellobiohydrolase activity.

The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence, which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.

The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.

A process using a multicomponent enzyme preparation to treat screened once refined pulps and reduces the specific energy consumption and / or increasing production while maintaining or increasing handsheet physical properties. The enzyme preparation has a major endoglucanase activity, a significant mannanase activity and a relatively small cellobiohydrolase activity. This enzyme mixture is prepared from a genetically modified strain of Trichoderma reseii.

A thermostable cellobiohydrolase including a cellobiohydrolase catalytic domain, the cellobiohydrolase catalytic domain including:(A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1;(B) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid of the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;(C) a polypeptide including an amino acid sequence having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;(D) a polypeptide including an amino acid sequence represented by SEQ ID NO: 3;(E) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid (with a proviso that cysteine residues at positions 291 and 296 in the amino acid sequence are excluded) of the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5; or(F) a polypeptide including an amino acid sequence (with a proviso that cysteine residues are present at positions 291 and 296) having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5.

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention discloses a cellobiohydrolase mutant. The activity of the cellobiohydrolase mutant provides a degradation function in cellulose degradation, an enzyme is a protein shown in a formula (a)or (b), theronine at a 389th site of an amino acid sequence shown in SEQ ID NO:1 of (a) is substituted by other amino acid, the other amino acid is lysine, arginine, aspartic acid, glutamic acid or glutamine, and an amino acid sequence of (b) in (a) is a protein which is derived by (a), is substituted, deleted or added with one or more amino acids and has cellobiohydrolase activity. The inventionalso discloses a composition, the composition contains one or several enzymes, and the enzymes are cellobiohydrolase mutants. The invention also discloses application of the cellobiohydrolase mutantor composition in cellulose hydrolysis. The cellobiohydrolase mutant disclosed by the invention has cellulose hydrolysis activity, and the mutant with the activity improved is obtained, so that the cellulose hydrolysis effect is improved.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention relates to a method for measuring the activity of cellobiohydrolase I in the subtropical forest soil, and especially relates to a method utilizing a multifunctional enzyme-linked immunosorbent assay (ELISA) instrument to measure the activity of cellobiohydrolase I in the subtropical forest soil. According to the provided method, nitrobenzene cellobioside is taken as the substrate, then the substrate is mixed with a soil sample for reactions in a water bath, and after the reactions, a multifunctional ELISA instrument is used to measure the reaction products. The provided measuring method has the advantages of simpleness, efficiency, and accuracy.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention discloses a corynespora cassicola CcTLS1 protein, a coding gene and application thereof. Experiments in the invention prove that: after the corynespora cassicola CcTLS1 gene is knocked out, the scab area of the obtained corynespora cassicola knockout mutant on cucumber leaves is greatly reduced compared with that of a wild strain; the mycelial growth speed of the corynespora cassicola knockout mutant is obviously lower than that of wild corynespora cassicola; and the activity of cellobiose hydrolase of the corynespora cassicola knockout mutant is obviously lower than that of the wild corynespora cassicola. The result shows that the deletion of the corynespora cassicola knockout mutant CcTLS1 gene can cause the decrease of the infection ability of the corynespora cassicola to the cucumber. The CcTLS1 gene and application thereof provided by the invention have important significance in prevention and control of cucumber corynespora leaf spot.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules.

The present invention relates to polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules.

The subject invention relates to novel compositions of neutral and / or alkaline cellulase and methods for obtaining neutral and / or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and / or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and / or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and / or alkaline cellulases. The neutral and / or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams. The present invention also relates to the isolation and purification of cellulase enzymes, having glucanase and cellobiohydrolase activity, and useful for stonewashing applications.

The polypeptide having the amino acid sequence shown in SEQ ID NO: 1 or using at least one method of deletion, addition, insertion or substitution, and one or more amino acids are deleted, added, inserted or replaced to obtain cellobiohydrolase activity of polypeptides.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Disclosed herein are polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. Disclosed herein are nucleic acid constructs, vectors and host cells comprise the polynucleotides as well as their uses, and methods of producing the polypeptides, catalytic domains, carbohydrate binding modules.

A thermostable cellobiohydrolase including a cellobiohydrolase catalytic domain, the cellobiohydrolase catalytic domain including:(A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1;(B) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid of the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;(C) a polypeptide including an amino acid sequence having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;(D) a polypeptide including an amino acid sequence represented by SEQ ID NO: 3;(E) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid (with a proviso that cysteine residues at positions 291 and 296 in the amino acid sequence are excluded) of the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5; or(F) a polypeptide including an amino acid sequence (with a proviso that cysteine residues are present at positions 291 and 296) having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5.

The present invention discloses a cellobiohydrolase mutant, whose activity provides the degradation function in cellulose degradation, and the enzyme is the following (a) or (b) protein: (a) as shown in SEQ ID NO: 1 Threonine at position 393 of the amino acid sequence is replaced by other amino acids, the other amino acids are lysine, arginine or glutamic acid, (b) the amino acid sequence in (a) has been substituted, deleted or added or a protein derived from (a) of several amino acids and having cellobiohydrolase activity. The invention also discloses a composition containing one or several enzymes. The invention also discloses the application of cellobiohydrolase mutant or composition in cellulose hydrolysis. The cellobiohydrolase mutant of the present invention has the activity of hydrolyzing cellulose, and a mutant with improved activity is obtained, and the effect of hydrolyzing cellulose is improved.

The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.